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44 results on '"Yin, Bohan"'

6. An Aggregation-Induced Emission-Based Dual Emitting Nanoprobe for Detecting Intracellular pH and Unravelling Metabolic Variations in Differentiating Lymphocytes

Author

Huang, Yingying, primary, Zhang, Qin, additional, Lam, Ching Ying Katherine, additional, Li, Chuanqi, additional, Yang, Chen, additional, Zhong, Zhiming, additional, Zhang, Ruolin, additional, Yan, Jiaxiang, additional, Chen, Jiareng, additional, Yin, Bohan, additional, Wong, Siu Hong Dexter, additional, and Yang, Mo, additional

Published
2024
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8. Nucleic Acid Armor: Fortifying RNA Therapeutics through Delivery and Targeting Innovations for Immunotherapy.

Author

Jiang, Yi, Jiang, Bolong, Wang, Zhenru, Li, Yuxi, Cheung, James Chung Wai, Yin, Bohan, and Wong, Siu Hong Dexter

Subjects
CYTOCHEMISTRY, GENETIC vectors, CELL receptors, T cells, CLICK chemistry
Abstract

RNA is a promising nucleic acid-based biomolecule for various treatments because of its high efficacy, low toxicity, and the tremendous availability of targeting sequences. Nevertheless, RNA shows instability and has a short half-life in physiological environments such as the bloodstream in the presence of RNAase. Therefore, developing reliable delivery strategies is important for targeting disease sites and maximizing the therapeutic effect of RNA drugs, particularly in the field of immunotherapy. In this mini-review, we highlight two major approaches: (1) delivery vehicles and (2) chemical modifications. Recent advances in delivery vehicles employ nanotechnologies such as lipid-based nanoparticles, viral vectors, and inorganic nanocarriers to precisely target specific cell types to facilitate RNA cellular entry. On the other hand, chemical modification utilizes the alteration of RNA structures via the addition of covalent bonds such as N-acetylgalactosamine or antibodies (antibody–oligonucleotide conjugates) to target specific receptors of cells. The pros and cons of these technologies are enlisted in this review. We aim to review nucleic acid drugs, their delivery systems, targeting strategies, and related chemical modifications. Finally, we express our perspective on the potential combination of RNA-based click chemistry with adoptive cell therapy (e.g., B cells or T cells) to address the issues of short duration and short half-life associated with antibody–oligonucleotide conjugate drugs. [ABSTRACT FROM AUTHOR]

Published
2024
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10. Magnetic-responsive upconversion luminescence resonance energy transfer (LRET) biosensor for ultrasensitive detection of SARS-CoV-2 spike protein

Author

Chen, Jiareng, primary, Ho, Willis Kwun Hei, additional, Yin, Bohan, additional, Zhang, Qin, additional, Li, Chuanqi, additional, Yan, Jiaxiang, additional, Huang, Yingying, additional, Hao, Jianhua, additional, Yi, Changqing, additional, Zhang, Yu, additional, Wong, Siu Hong Dexter, additional, and Yang, Mo, additional

Published
2023
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15. A Multilayered Mesoporous Gold Nanoarchitecture for Ultraeffective Near‐Infrared Light‐Controlled Chemo/Photothermal Therapy for Cancer Guided by SERS Imaging (Small 6/2023)

Author

Yin, Bohan, primary, Ho, Willis Kwun Hei, additional, Xia, Xinyue, additional, Chan, Cecilia Ka Wing, additional, Zhang, Qin, additional, Ng, Yip Ming, additional, Lam, Ching Ying Katherine, additional, Cheung, James Chung Wai, additional, Wang, Jianfang, additional, Yang, Mo, additional, and Wong, Siu Hong Dexter, additional

Published
2023
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18. An AIEgen/graphene oxide nanocomposite (AIEgen@GO)‐based two‐stage “turn‐on” nucleic acid biosensor for rapid detection of SARS‐CoV‐2 viral sequence

Author

Zhang, Qin, primary, Yin, Bohan, additional, Hao, Jianhua, additional, Ma, Linjie, additional, Huang, Yingying, additional, Shao, Xueying, additional, Li, Chuanqi, additional, Chu, Zhiqin, additional, Yi, Changqing, additional, Wong, Siu Hong Dexter, additional, and Yang, Mo, additional

Published
2022
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23. A CRISPR-Cas12a integrated SERS nanoplatform with chimeric DNA/RNA hairpin guide for ultrasensitive nucleic acid detection

Author

Yin, Bohan, primary, Zhang, Qin, additional, Xia, Xinyue, additional, Li, Chuanqi, additional, Ho, Willis Kwun Hei, additional, Yan, Jiaxiang, additional, Huang, Yingying, additional, Wu, Honglian, additional, Wang, Pui, additional, Yi, Changqing, additional, Hao, Jianhua, additional, Wang, Jianfang, additional, Chen, Honglin, additional, Wong, Siu Hong Dexter, additional, and Yang, Mo, additional

Published
2022
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24. Error analysis driven network modification for surgical tools detection in laparoscopic frames.

Author

Yin, Bohan, Wang, Shengsheng, Lu, Shuzhen, Wang, Guangyao, and Dong, Liyan

Subjects
*ADVERSE health care events, *MINIMALLY invasive procedures, *LAPAROSCOPIC surgery, *SURGICAL robots, *JUDGMENT (Psychology)
Abstract

In minimally invasive laparoscopic surgery, accurate detection of the location and specific category of surgical tools assists the surgeon in making a correct objective judgment of the current surgical outcome and alerts to these possible adverse medical events. In this paper, an analyzing error method is proposed for revealing the specific sources of error in previous work. Then we confirm the main bottlenecks of the current methods with a high level of missing error and localization error. To reduce these errors, we render the backbone with a three‐dimensional attention mechanism and adopt a double‐headed detection head design to replace the single detection head. Moreover, we propose an enhanced multilayer perceptron called Mconv to enhance the localization branch of the double‐headed detection head. Our experiments evaluate our improved approach by our proposed error analysis method on a pubic dataset m2cai16‐tool‐locations, showing that our method yield remarkable detection accuracy than others. [ABSTRACT FROM AUTHOR]

Published
2023
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29. Intrapulmonary Cellular-Level Distribution of Inhaled Nanoparticles with Defined Functional Groups and Its Correlations with Protein Corona and Inflammatory Response

Author

Yin, Bohan, primary, Chan, Cecilia Ka Wing, additional, Liu, Shaorui, additional, Hong, Huiling, additional, Wong, Siu Hong Dexter, additional, Lee, Leo Kit Cheung, additional, Ho, Lok Wai Cola, additional, Zhang, Lei, additional, Leung, Ken Cham-Fai, additional, Choi, Paul Cheung-Lung, additional, Bian, Liming, additional, Tian, Xiao Yu, additional, Chan, Man Nin, additional, and Choi, Chung Hang Jonathan, additional

Published
2019
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30. Anisotropic Nanoscale Presentation of Cell Adhesion Ligand Enhances the Recruitment of Diverse Integrins in Adhesion Structures and Mechanosensing‐Dependent Differentiation of Stem Cells

Author

Wong, Siu Hong Dexter, primary, Yin, Bohan, additional, Yang, Boguang, additional, Lin, Sien, additional, Li, Rui, additional, Feng, Qian, additional, Yang, Hongrong, additional, Zhang, Lei, additional, Yang, Zhengmeng, additional, Li, Gang, additional, Choi, Chung Hang Jonathan, additional, and Bian, Liming, additional

Published
2019
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31. Promoting the Delivery of Nanoparticles to Atherosclerotic Plaques by DNA Coating

Author

Zhang, Lei, primary, Tian, Xiao Yu, additional, Chan, Cecilia K. W., additional, Bai, Qianqian, additional, Cheng, Chak Kwong, additional, Chen, Francis M., additional, Cheung, Maggie S. H., additional, Yin, Bohan, additional, Yang, Hongrong, additional, Yung, Wing-Yin, additional, Chen, Zhong, additional, Ding, Fei, additional, Leung, Ken Cham-Fai, additional, Zhang, Chuan, additional, Huang, Yu, additional, Lau, James Y. W., additional, and Choi, Chung Hang Jonathan, additional

Published
2018
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35. Effect of Surface Modification with Hydrocarbyl Groups on the Exocytosis of Nanoparticles

Author

Ho, Lok Wai Cola, Yin, Bohan, Dai, Gaole, and Choi, Chung Hang Jonathan

Abstract

Designing nanoparticles (NPs) with desirable cell type-specific exocytosis properties, say promoting their exocytosis from scavenging cell types (e.g., macrophages and endothelial cells) or suppressing their exocytosis from target disease cell types (e.g., cancer cells), improves the application of nanomedicines. However, the design parameters available for tuning the exocytosis of NPs remain scarce in the “nano-cell” literature. Here, we demonstrate that surface modification of NPs with hydrocarbyl functional groups, commonly found in biomolecules and NP-based drug carriers, is a critical parameter for tuning the exocytosis of NPs from RAW264.7 macrophages, C166 endothelial cells, and HeLa epithelial cancer cells. To exclude the effect of hydrophobicity, we prepare a collection of hydrophilic NPs that bear a gold NP (AuNP) core, a dense polyethylene glycol (PEG) shell, and different types of hydrocarbyl groups (X) that are attached to the distal end of the PEG strands (termed “Au@PEG-X NPs”). For all three cell types tested, modification of NPs with straight-chain dodecane leads to a >10-fold increase in the level of cellular uptake, drastically higher than those of all other types of X tested. However, the probability of exocytosis of NPs significantly depends on the types of cell and X. Notably, NPs modified with cyclododecanes are most likely to be exocytosed by RAW264.7 and C166 cells (but not HeLa cells), accompanied by the release of intralumenal vesicles to the extracellular milieu. These data suggest a reductionist approach for rationally assembling bionanomaterials for nanomedicine applications by using hydrocarbyl functional groups as building blocks.

Published
2021
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37. A Biomimetic Upconversion Nanoreactors for Near-infrared Driven H2Release to Inhibit Tauopathy in Alzheimer's Disease Therapy

Author

Zhang, Qin, Li, Chuanqi, Yin, Bohan, Yan, Jiaxiang, Gu, Yutian, Huang, Yingying, Chen, Jiareng, Lao, Xinyue, Hao, Jianhua, Yi, Changqing, Zhou, Yi, Wai Cheung, James Chung, Dexter Wong, Siu Hong, and Yang, Mo

Abstract

Abnormal hyperphosphorylation of tau protein is a principal pathological hallmark in the onset of neurodegenerative disorders, such as Alzheimer's disease (AD), which can be induced by an excess of reactive oxygen species (ROS). As an antioxidant, hydrogen gas (H2) has the potential to mitigate AD by scavenging highly harmful ROS such as •OH. However, conventional administration methods of H2face significant challenges in controlling H2release on demand and fail to achieve effective accumulation at lesion sites. Herein, we report artificial nanoreactors that mimic natural photosynthesis to realize near-infrared (NIR) light-driven photocatalytic H2evolution in situ. The nanoreactors are constructed by biocompatible crosslinked vesicles (CVs) encapsulating ascorbic acid and two photosensitizers, chlorophyll a(Chla) and indoline dye (Ind). In addition, platinum nanoparticles (Pt NPs) serve as photocatalysts and upconversion nanoparticles (UCNP) act as light-harvesting antennas in the nanoreacting system, and both attach to the surface of CVs. Under NIR irradiation, the nanoreactors release H2in situ to scavenge local excess ROS and attenuate tau hyperphosphorylation in the AD mice model. Such NIR-triggered nanoreactors provide a proof-of-concept design for the great potential of hydrogen therapy against AD.

Published
2024
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41. Toward Understanding in VivoSequestration of Nanoparticles at the Molecular Level

Author

Yin, Bohan, Li, Kin Hei Kelvin, Ho, Lok Wai Cola, Chan, Cecilia Ka Wing, and Choi, Chung Hang Jonathan

Abstract

A longstanding and widely accepted bottleneck in the targeted delivery of intravenously injected nanoparticles lies in their clearance by macrophages in the liver and spleen. In this Perspective, we call for deeper understanding of the critical role of endothelial cells in the sequestration of nanoparticles in vivo. In this issue of ACS Nano, Campbell et al. used a combination of real-time imaging and genome-editing methods to demonstrate that stabilin-2 is an important receptor for removing anionic liposomes from blood circulation in a zebrafish model. Such mechanistic insights at the molecular level will provide a more holistic picture of the in vivosequestration of administered nanoparticles beyond the cellular level and pose valuable design considerations for redistributing nanoparticles in vivo.

Published
2018
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43. Optofluidic chip with directly printed polymer optical waveguide Mach-Zehnder interferometer sensors for label-free biodetection.

Author

Wang H, Chen Z, Li T, Xie H, Yin B, Wong SHD, Shi Y, and Zhang AP

Abstract

Optofluidic devices hold great promise in biomedical diagnostics and testing because of their advantages of miniaturization, high sensitivity, high throughput, and high scalability. However, conventional silicon-based photonic chips suffer from complicated fabrication processes and less flexibility in functionalization, thus hindering their development of cost-effective biomedical diagnostic devices for daily tests and massive applications in responding to public health crises. In this paper, we present an optofluidic chip based on directly printed polymer optical waveguide Mach-Zehnder interferometer (MZI) sensors for label-free biomarker detection. With digital ultraviolet lithography technology, high-sensitivity asymmetric MZI microsensors based on a width-tailored optical waveguide are directly printed and vertically integrated with a microfluidic layer to make an optofluidic chip. Experimental results show that the sensitivity of the directly printed polymer optical waveguide MZI sensor is about 1695.95 nm/RIU. After being modified with capture molecules, i.e., goat anti-human immunoglobulin G (IgG), the polymer optical waveguide MZI sensors can on-chip detect human IgG at the concentration level of 1.78 pM. Such a polymer optical waveguide-based optofluidic chip has the advantages of miniaturization, cost-effectiveness, high sensitivity, and ease in functionalization and thus has great potential in the development of daily available point-of-care diagnostic and testing devices., Competing Interests: There are no conflicts to declare., (© 2024 Optica Publishing Group.)

Published
2024
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44. The Interplay Between Epigenetic Regulation and CD8 + T Cell Differentiation/Exhaustion for T Cell Immunotherapy.

Author

Wong WK, Yin B, Lam CYK, Huang Y, Yan J, Tan Z, and Wong SHD

Abstract

Effective immunotherapy treats cancers by eradicating tumourigenic cells by activated tumour antigen-specific and bystander CD8 + T-cells. However, T-cells can gradually lose cytotoxicity in the tumour microenvironment, known as exhaustion. Recently, DNA methylation, histone modification, and chromatin architecture have provided novel insights into epigenetic regulations of T-cell differentiation/exhaustion, thereby controlling the translational potential of the T-cells. Thus, developing strategies to govern epigenetic switches of T-cells dynamically is critical to maintaining the effector function of antigen-specific T-cells. In this mini-review, we 1) describe the correlation between epigenetic states and T cell phenotypes; 2) discuss the enzymatic factors and intracellular/extracellular microRNA imprinting T-cell epigenomes that drive T-cell exhaustion; 3) highlight recent advances in epigenetic interventions to rescue CD8 + T-cell functions from exhaustion. Finally, we express our perspective that regulating the interplay between epigenetic changes and transcriptional programs provides translational implications of current immunotherapy for cancer treatments., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Wong, Yin, Lam, Huang, Yan, Tan and Wong.)

Published
2022
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