1. Antibiotic-Fe3O4 nanoparticles with highly efficient catalytic activity for enhanced chemiluminescence detection of tetracyclines residues in foods
- Author
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Fei Yu, Binghua Fan, Yilin Chai, Yue Liu, Jiaxiang Wang, Yueqi Liao, Songcheng Yu, Jia Wang, Yongjun Wu, and Yilin Wang
- Subjects
Tetracyclines detection ,Fe3O4 nanoparticles ,Nanozyme ,Enhanced chemiluminescence analysis ,Nutrition. Foods and food supply ,TX341-641 ,Food processing and manufacture ,TP368-456 - Abstract
Tetracyclines (TCs) are the most commonly antimicrobial agents that used in livestock production worldwide. It is important to supervise tetracyclines residues in food for environmental monitoring and food safety. In this study, a novel, label-free chemiluminescence (CL) assay without antibody was established. Fe3O4 NPs could facilitate the CL interaction between luminol and H2O2. Interestingly, TCs could enhance the catalytic ability of Fe3O4 NPs and result in a further amplification of the CL intensity. The CL intensity varied linearly with the concentration of tetracycline (TC), oxytetracycline (OTC), chlortetracycline (CTC), and ranging from 10–2400, 10–2800, and 5–2100 nmol/L, respectively; The limits of detection were 4 nmol/L for TC, 6 nmol/L for OTC, and 2 nmol/L for CTC. This CL strategy was applied successfully in testing three TCs residues in milk, eggs and honey samples with more sensitive results, which provided an alternative strategy for monitoring the correct use of TCs.
- Published
- 2024
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