187 results on '"Yijun Fan"'
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2. Machine learning predicts the serum PFOA and PFOS levels in pregnant women: Enhancement of fatty acid status on model performance
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Lin Tao, Weitian Tang, Zhicai Xia, Bing Wu, Heng Liu, Juanjuan Fu, Qiufang Lu, Liyan Guo, Chang Gao, Qiang Zhou, Yijun Fan, De-Xiang Xu, and Yichao Huang
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PFAS ,Machine learning ,Pregnant woman ,Dietary exposure ,Fatty acid ,Exposure prediction ,Environmental sciences ,GE1-350 - Abstract
Human exposure to per- and polyfluoroalkyl substances (PFASs) has received considerable attention, particularly in pregnant women because of their dramatic changes in physiological status and dietary patterns. Predicting internal PFAS exposure in pregnant women, based on external and relevant parameters, has not been investigated. Here, machine learning (ML) models were developed to predict the serum concentrations of PFOA and PFOS in a large population of 588 pregnant participants. Dietary exposure characteristics, demographic parameters, and in particular, serum fatty acid (FA) data were used for the model development. The fitting results showed that the inclusion of FAs as covariates significantly improved the performance of the ML models, with the random forest (RF) model having the best predictive performance for PFOA (R2 = 0.33, MAE = 1.51 ng/mL, and RMSE = 1.89 ng/mL) and PFOS (R2 = 0.12, MAE = 2.65 ng/mL, and RMSE = 3.37 ng/mL). The feature importance analysis revealed that serum FAs greatly affected PFOA concentration in the pregnant women, with saturated FAs being associated with decreased PFOA levels and unsaturated FAs with increased levels. Comparison with one-compartment pharmacokinetic model further demonstrated the advantage of the ML models in predicting PFAS exposure in pregnant women. Our models correlate for the first time blood chemical concentrations with human FA status using ML, introducing a novel perspective on predicting PFAS levels in pregnant women. This study provides valuable insights concerning internal exposure of PFASs generated from external exposure, and contributes to risk assessment and management in pregnant populations.
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- 2024
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3. Entrepreneurial mental health in the wake of COVID-19 in China with an emphasis on attention deficit hyperactivity disorder (ADHD) and dyslexia analysis
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Yijun Fan, Yuanzhe Li, Zhengyuan Dong, May Ong, and James Hope
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Entrepreneurship in China ,Mental health impact ,COVID-19 pandemic ,Attention deficit hyperactivity disorder (ADHD) ,Dyslexia ,Medicine ,Science - Abstract
Abstract The COVID-19 pandemic has precipitated a global mental health crisis, with a particularly pronounced impact on the entrepreneurial sector. This paper presents a comparative analysis of mental health challenges among entrepreneurs in China during the pandemic, with a specific focus on attention deficit hyperactivity disorder (ADHD) and Dyslexia. The study assesses the prevalence of ADHD and dyslexia symptoms among established and emerging entrepreneurs in China, finding notable occurrences within this group. The research also examines the self-care practices of these entrepreneurs, shedding light on their approaches during the pandemic period. The findings highlight a complex interplay between mental health issues and entrepreneurial activities, suggesting that certain ADHD and dyslexia traits may offer unexpected benefits in the entrepreneurial realm. These insights are critical for developing supportive frameworks that leverage the strengths of neurodiverse entrepreneurs while mitigating associated challenges, especially in a post-pandemic economic landscape. The study concludes with policy and practice recommendations to bolster the wellbeing and resilience of entrepreneurs facing the multifaceted impacts of the pandemic.
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- 2024
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4. Gestational PFAS exposure and newborn size: The modifying effect of cord blood fatty acids
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Chang Gao, Lin Luo, Yijun Fan, Liyan Guo, Lijuan Guo, Lin Tao, Fangbiao Tao, De-Xiang Xu, Robert A. Gibson, Maria Makrides, Hua Wang, and Yichao Huang
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Per- and polyfluoroalkyl substance ,Gestational exposure ,Fetal nutrition ,Fatty acid status ,Newborn size ,Environmental sciences ,GE1-350 ,Environmental technology. Sanitary engineering ,TD1-1066 - Abstract
Per- and polyfluoroalkyl substances (PFASs) can disrupt lipid metabolism, and changes in cord blood fatty acid composition have been observed in small newborns. Emerging evidence suggests that exposure to PFASs during pregnancy is linked to decreased newborn size, although the evidence is not consistent. The modifying effect of fatty acids on the associations of gestational PFAS exposure with newborn size is still unknown. Here we show that the nutritional status of the fetus, as indicated by the level of fatty acids in the cord blood, mitigates the adverse effects of gestational PFAS exposure on the size of the newborn. Our study confirms the adverse developmental effects of PFASs and identifies emerging short-chain PFASs as the primary drivers of reduced newborn size, despite their lower exposure burden compared to legacy PFASs. Additionally, we find the protective role of cord blood fatty acids, suggesting potential strategies for mitigating the detrimental effects of emerging environmental exposures on human health. Our findings provide new evidence of the potential toxicity of emerging PFASs and call for further toxicity evaluations of these pollutants for regulatory purposes. Future studies should consider the complex interaction between exposure and nutrition within the human body, particularly during the first thousand days of life, to promote lifelong health.
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- 2024
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5. Experience of naturally occurring peer support among people using psychiatric day-care in China: an interpretative phenomenology approach
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Yijun Fan, Xiao Liu, and Conghong Li
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peer support ,mental rehabilitation ,community mental health services ,interpretative phenomenological analysis ,China ,Psychiatry ,RC435-571 - Abstract
IntroductionChina presently does not have an established peer support system, and the efforts of peer support workers are not acknowledged in the context of the growing global trend of community-based mental rehabilitation. This study aims to examine the first-hand experiences of persons who participate in psychiatric day-care facilities in Shanghai, China, and receive support from their peers. The goal is to gain a better understanding of how these informal peer support programs function and provide valuable knowledge for the establishment of more structured peer support programs that align with Chinese social culture.MethodsA total of 14 participants attending psychiatric day-care centres were selected for face-to-face semi-structured in-depth interviews using snowball sampling. The interviews took place between July 2021 and February 2022. The text data of the interview were acquired through transcription and then augmented using the interview scripts and additional resources. The data were analysed using interpretive phenomenological analysis until the themes reached saturation.ResultsA total of three overarching themes and eight corresponding sub-themes were produced: 1. reconstructing a social network: an ordinary interpersonal connection, becoming and conducting oneself, proceeding the process of adaptation in the company of peers; 2. balance and multiple roles within the relationship: selective self-exposure, managing proximity and distance; 3. sense of meaning and sense of community: supporting others while empowering oneself, love as expanding consciousness, advocating for the notion of group identity.DiscussionThis study is the initial examination of the contact and naturally occurring peer support that takes place among individuals in psychiatric day-care centres in China. The study’s findings revealed that participants interact with others who have undergone similar conditions in the day-care setting, enabling them to rebuild an important social network. It is crucial to consider the possible benefits of peer support, assess the obstacles, and facilitate the personal recovery of individuals with mental disorders using the theory of recovery.
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- 2024
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6. Beyond play: a comparative study of multi-sensory and traditional toys in child education
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Yijun Fan, Daphne Khee Chong, and Yuanzhe Li
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multi-sensory theory ,educational toys ,engagement metrics ,learning outcomes ,child education ,Education (General) ,L7-991 - Abstract
As educational paradigms evolve, the integration of multi-sensory theory into the design of children’s educational toys presents a promising avenue for enhancing learning experiences. This paper explores the efficacy of multi-sensory toys in improving children’s attraction, interest, and learning efficiency through a systematic review and a pilot empirical study. The study specifically assesses the hypothesis that multi-sensory educational toys significantly increase children’s engagement and learning outcomes compared to traditional toys. Conducted with a diverse group of children aged 3–6 in Guangzhou and Shenzhen, China, the research employs both quantitative and qualitative methodologies, including engagement metrics and observational studies. The findings suggest that multi-sensory toys not only hold the potential to augment learning experiences but also require careful consideration of individual learning styles and preferences. The paper concludes with a discussion on the implications for future research and toy design, emphasizing the need for continued innovation and personalization in the development of educational toys to cater to the multifaceted needs of young learners.
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- 2024
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7. Vertically stratified methane, nitrogen and sulphur cycling and coupling mechanisms in mangrove sediment microbiomes
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Lu Qian, Xiaoli Yu, Hang Gu, Fei Liu, Yijun Fan, Cheng Wang, Qiang He, Yun Tian, Yisheng Peng, Longfei Shu, Shanquan Wang, Zhijian Huang, Qingyun Yan, Jianguo He, Guangli Liu, Qichao Tu, and Zhili He
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Vertical distribution ,Metagenome sequencing analysis ,Mangrove sediment ,Methane/nitrogen/sulphur cycling ,Metagenome-assembled genome ,Coupling mechanism ,Microbial ecology ,QR100-130 - Abstract
Abstract Background Mangrove ecosystems are considered as hot spots of biogeochemical cycling, yet the diversity, function and coupling mechanism of microbially driven biogeochemical cycling along the sediment depth of mangrove wetlands remain elusive. Here we investigated the vertical profile of methane (CH4), nitrogen (N) and sulphur (S) cycling genes/pathways and their potential coupling mechanisms using metagenome sequencing approaches. Results Our results showed that the metabolic pathways involved in CH4, N and S cycling were mainly shaped by pH and acid volatile sulphide (AVS) along a sediment depth, and AVS was a critical electron donor impacting mangrove sediment S oxidation and denitrification. Gene families involved in S oxidation and denitrification significantly (P < 0.05) decreased along the sediment depth and could be coupled by S-driven denitrifiers, such as Burkholderiaceae and Sulfurifustis in the surface sediment (0–15 cm). Interestingly, all S-driven denitrifier metagenome-assembled genomes (MAGs) appeared to be incomplete denitrifiers with nitrate/nitrite/nitric oxide reductases (Nar/Nir/Nor) but without nitrous oxide reductase (Nos), suggesting such sulphide-utilizing groups might be an important contributor to N2O production in the surface mangrove sediment. Gene families involved in methanogenesis and S reduction significantly (P < 0.05) increased along the sediment depth. Based on both network and MAG analyses, sulphate-reducing bacteria (SRB) might develop syntrophic relationships with anaerobic CH4 oxidizers (ANMEs) by direct electron transfer or zero-valent sulphur, which would pull forward the co-existence of methanogens and SRB in the middle and deep layer sediments. Conclusions In addition to offering a perspective on the vertical distribution of microbially driven CH4, N and S cycling genes/pathways, this study emphasizes the important role of S-driven denitrifiers on N2O emissions and various possible coupling mechanisms of ANMEs and SRB along the mangrove sediment depth. The exploration of potential coupling mechanisms provides novel insights into future synthetic microbial community construction and analysis. This study also has important implications for predicting ecosystem functions within the context of environmental and global change. Video Abstract
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- 2023
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8. Structural Characterization and Immunoenhancing Properties of Polysaccharide CPTM-P1 from Taxus media
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Jiangtao Fan, Xiong Huang, Mengke Dou, Shuqin Tang, Gang Wang, Yijun Fan, Aoxue Luo, and Yong Wang
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Taxus media ,polysaccharide ,structural characterization ,immunoenhancing properties ,Organic chemistry ,QD241-441 - Abstract
Polysaccharides extracted from Taxus media hrough an aqueous method were further refined by removing proteins via the Sevag technique and purified by dialysis. The separation of these polysaccharides was accomplished using a DEAE-cellulose chromatog-raphy column, yielding two distinct fractions, named CPTM-P1 and CPTM-P2. Notably, CPTM-P1 emerged as the primary polysaccharide component within Taxus media. Consequently, a comprehensive analysis focusing exclusively on CPTM-P1 was undertaken. The molecular weight of CPTM-P1 was established through gel permeation chromatography (GPC), and its monosaccharide composition was deciphered using HPLC-MS. The structure was further elucidated through nuclear magnetic resonance (NMR) spectroscopy. The molecular weight of CPTM-P1 was determined to be 968.7 kDa. The monosaccharide composition consisted of galactose (Gal), arabinose (Ara), galacturonic acid (Gal-UA), glucose (Glc), rhamnose (Rha), xylose (Xyl), mannose (Man), fucose (Fuc), glucuronic acid (Glc-UA), and ribose (Rib). The proportional distribution of these components was 30.53%, 22.00%, 5.63%, 11.67%, 11.93%, 1.69%, 8.50%, 1.23%, 5.63%, and 1.17%, respectively. This confirmed CPTM-P1 as an acidic heteropolysaccharide with a glycuronic acid backbone. Moreover, CPTM-P1 showed immunoenhancing properties, effectively augmenting the secretion of nitric oxide and cytokines (TNF-α, IL-1β, and IL-6). Additionally, it significantly enhances the phagocytic capacity of RAW264.7 cells. These findings underscore the potential application of these polysaccharides in functional foods and pharmaceuticals, providing a solid scientific basis for further exploration and utilization of Taxus media polysaccharides.
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- 2024
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9. Ultrasonic extraction, structural modification and gastric mucosal cells protective activity of a polysaccharide from Dendrobium denneanum
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Yijun Fan, Jie Ma, Gang Wang, Xuebing Li, Yuanyuan Liu, Erya Xu, and Aoxue Luo
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Ultrasonic extraction ,Dendrobium denneanum ,Polysaccharide ,Low-temperature plasma ,Structural characteristics ,GES-1 cells ,Chemistry ,QD1-999 - Abstract
Optimize the ultrasonic extraction process of polysaccharides from Dendrobium denneanum by Response surface design (RSM). The main polysaccharide (DP40) from was separated by anion exchange chromatography, and its structure was modified by low-temperature plasma. The results showed that the polysaccharide extraction rate of ultrasonic was 22.98 ± 0.97%, which was significantly higher than water extraction method(P
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- 2023
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10. Developmental toxicity of perfluorohexane sulfonate at human relevant dose during pregnancy via disruption in placental lipid homeostasis
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Wencong Yao, Jingjing Xu, Weitian Tang, Chang Gao, Lin Tao, Jie Yu, Jia Lv, Hua Wang, Yijun Fan, De-Xiang Xu, and Yichao Huang
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PFHxS ,Gestational exposure ,Developmental toxicology ,Placenta ,Lipid homeostasis ,Environmental sciences ,GE1-350 - Abstract
Perfluorohexyl sulfonate (PFHxS) is the third most abundant per- and polyfluoroalkyl substances and its developmental toxicity remains very poorly understood. Here, pregnant mice exposed to PFHxS at human relevant dose showed increased fetal death incidence in the high-dose PFHxS-H group (P
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- 2023
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11. Enhancement of Macrophage Immunity against Chlamydial Infection by Natural Killer T Cells
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Ying Peng, Sai Qiao, Hong Wang, Sudhanshu Shekhar, Shuhe Wang, Jie Yang, Yijun Fan, and Xi Yang
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Chlamydia ,macrophage ,iNKT cells ,M1 ,JAK/STAT signalling pathway ,Cytology ,QH573-671 - Abstract
Lung macrophage (LM) is vital in host defence against bacterial infections. However, the influence of other innate immune cells on its function, including the polarisation of different subpopulations, remains poorly understood. This study examined the polarisation of LM subpopulations (monocytes/undifferentiated macrophages (Mo/Mφ), interstitial macrophages (IM), and alveolar macrophages (AM)). We further assessed the effect of invariant natural killer T cells (iNKT) on LM polarisation in a protective function against Chlamydia muridarum, an obligate intracellular bacterium, and respiratory tract infection. We found a preferentially increased local Mo/Mφ and IMs with a significant shift to a type-1 macrophage (M1) phenotype and higher expression of iNOS and TNF-α. Interestingly, during the same infection, the alteration of macrophage subpopulations and the shift towards M1 was much less in iNKT KO mice. More importantly, functional testing by adoptively transferring LMs isolated from iNKT KO mice (iNKT KO-Mφ) conferred less protection than those isolated from wild-type mice (WT-Mφ). Further analyses showed significantly reduced gene expression of the JAK/STAT signalling pathway molecules in iNKT KO-Mφ. The data show an important role of iNKT in promoting LM polarisation to the M1 direction, which is functionally relevant to host defence against a human intracellular bacterial infection. The alteration of JAK/STAT signalling molecule gene expression in iNKT KO-Mφ suggests the modulating effect of iNKT is likely through the JAK/STAT pathway.
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- 2024
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12. Heat Stress Alleviation by Exogenous Calcium in the Orchid Dendrobium nobile Lindl: A Biochemical and Transcriptomic Analysis
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Yijun Fan, Jie Ma, Yuanyuan Liu, Xueyan Tan, Xuebing Li, Erya Xu, Linlong Xu, and Aoxue Luo
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Dendrobium nobile ,heat stress ,exogenous calcium ,transcriptome ,gene expression ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
The growth of Dendrobium nobile is sensitive to heat stress. To find an effective method for enhancing heat tolerance, this study investigated the relieving effect of exogenous calcium at different concentrations (0 mmol/L, 5 mmol/L, 10 mmol/L, 15 mmol/L, 20 mmol/L CaCl2) on heat stress in D. nobile. Principal component analysis was used to screen the optimal exogenous calcium concentration, and transcriptome analysis was used to reveal its possible heat tolerance mechanism. The results showed that compared with the T0, a 10 mmol/L calcium treatment: increased the average leaf length, leaf width, plant height, and fresh matter accumulation of D. nobile by 76%, 103.39%, 12.97%, and 12.24%, respectively (p < 0.05); significantly increased chlorophyll a (Chla), chlorophyll b (Chlb), carotenoids(Car), ascorbic acid (ASA), glutathione (GSH), and flavonoids by 15.72%, 8.54%, 11.88%, 52.17%, 31.54%, and 36.12%, respectively; and effectively enhanced the enzyme activity of the antioxidant system, increasing superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) by 1.38, 1.61, and 2.16 times, respectively (p < 0.05); At the same time, the treatment can effectively reduce the yellow leaf rate and defoliation rate of D. nobile under heat stress. The principal component analysis method and membership function were used to calculate the D value to rank the relief effects of each calcium treatment group, and the results also showed that 10 mmol/L CaCl2 had the best relief effect. Transcriptomics testing identified 7013 differentially expressed genes, of which 2719 were upregulated, and 294 were downregulated. Among them, genes such as HSPA1s, HSP90A, HSPBP1, ATG8, COMT, REF1, E1.11.1.7, along with transcription factors such as MYB, bHLH, WRKY, and NAC, formed the network of tolerance to heat stress in D. nobile. This study provides new insights for improving the cultivation techniques of D. nobile.
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- 2023
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13. Isolation, Structural Characteristics Analysis of a Vigna unguiculata Polysaccharide VUP80-3 and Its Protective Effect on GES-1 Cells In Vitro
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Yijun Fan, Erya Xu, Jie Ma, Xuebing Li, Yuanyuan Liu, Linlong Xu, and Aoxue Luo
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Vigna unguiculata ,polysaccharide ,structural characterization ,gastric mucosal cells ,Organic chemistry ,QD241-441 - Abstract
Cowpea (Vigna unguiculata) is one of the main edible legume vegetables in China, and it can improve spleen and stomach function. A polysaccharide component (VUP80-3) has been isolated from V. unguiculata in this study. The average molecular weight of VUP80-3 is 6.43 × 104 Da, and the main monosaccharide group is glucose. The mass ratio of monosaccharide groups in the polysaccharide was glucose:galactose:arabinose:rhamnose:xylose:mannose:fucose = 152.36:24.50:16.53:8.13:1.26:0.97:0.82. NMR analysis showed that VUP80-3 has →4)-α-D-Galp (1→ and →4)-α-D-Glcp(1→ main chain and →3,4)-β-D-Glcp(1→, →4,6)-α-D-Glcp(1→ branch chains, and the terminal sugar is α-D-Glcp(1→. Biological activity test results showed that VUP80-3 at 1000 μg·mL−1 significantly increased the activity of ethanol injured GES-1 cells (p < 0.01) and significantly reduced reactive oxygen species (ROS) in ethanol injured GES-1 cells and inflammatory factors (IL-8, IL-1β and TNF-α,) in GES-1 cells. This compound also reduced the apoptosis rate (p < 0.05), thereby significantly reducing the oxidative damage caused by ethanol in GES-1 cells. Therefore, VUP80-3 is a potential drug to protect the gastric mucosa from damage.
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- 2023
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14. Research on Product Yield Prediction and Benefit of Tuning Diesel Hydrogenation Conversion Device Based on Data-Driven System
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Qianqian Zheng, Yijun Fan, Zhi Zhou, Hongbo Jiang, and Xiaolong Zhou
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RLG process ,deep neural network ,yield prediction model ,genetic algorithm ,benefit optimization model ,Technology - Abstract
In the refining process, a large amount of data are generated in daily production, and how to make full use of these data to improve the accuracy of simulation is the key to improving the operation level of refineries. At the same time, with the increasing environmental regulations and the improvement of gasoline and diesel quality standards, the ratio of diesel to gasoline is also changing with people’s demand for fuel consumption. Catalytic cracking light cycle oil (LCO) hydrogenation conversion technology (react LCO into gasoline, RLG) can produce modified diesel with high-octane gasoline, a high cetane number, and a low sulfur content, which improves the added value of the product. In this article, based on the production and operation data of a 1 million tons/year RLG device, a device yield prediction model was established using a deep neural network (DNN) algorithm, and the model was further optimized using a genetic algorithm (GA) to maximize the economic benefits of the device. As a result, the gasoline production yield increased by more than 3%. The experimental results show that the established model has a good reference value for improving the economic benefits of the RLG device.
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- 2023
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15. Transcriptional and Physiological Analysis Reveal New Insights into the Regulation of Fertilization (N, P, K) on the Growth and Synthesis of Medicinal Components of Dendrobium denneanum
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Yijun Fan, Erya Xu, Gang Wang, Dingxin He, Jie Ma, Yuanyuan Liu, Xuebing Li, and Aoxue Luo
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Dendrobium denneanum ,transcriptome ,fertilization ,orthogonal experimental design ,medicinal compounds ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
Dendrobium denneanum is an important medicinal and ornamental plant. Its ornamental and medicinal values are affected by its vegetative growth conditions and chemical composition accumulation. This study adopted an orthogonal experimental design to treat D. denneanum with nine different levels of nitrogen (N), potassium (K), and phosphorus (P). The morphological indicators of the plant were positively correlated with the nitrogen concentration. The polysaccharide content was the highest at 1500 mg·L−1 nitrogen and 3000 mg·L−1 phosphorous and was 26.84% greater than the control. The flavonoid content increased by 36.2% at 500 mg·L−1 nitrogen, 2000 mg·L−1 phosphorous, and 300 mg·L−1 potassium. Principal component score analysis showed that nitrogen had the most significant impact on the various indicators of D. denneanum, followed by phosphorus and potassium. The comprehensive score showed that the T9 treatment (N: 1500 mg·L−1, P: 3000 mg·L−1, K: 500 mg·L−1) had the strongest effect on D. denneanum. Transcriptional analysis showed that compared with the control, the T9 treatment led to 2277 differentially expressed genes (1230 upregulated and 1047 downregulated). This includes fifteen genes enriched in the MAPK signaling pathway, five genes in phenylpropanoid biosynthesis, and two genes in flavonoid biosynthesis. These genes may be involved in regulating plant growth and the biosynthesis of polysaccharides and flavonoids. This study provides guidance for the optimal use of N, P, and K in the cultivation of D. denneanum.
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- 2023
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16. Assessment of the Bioaccessibility of Carotenoids in Goji Berry (Lycium barbarum L.) in Three Forms: In Vitro Digestion Model and Metabolomics Approach
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Ziying Hu, Yanan Ma, Jun Liu, Yijun Fan, Anran Zheng, Pengyan Gao, Liang Wang, and Dunhua Liu
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goji berry ,bioaccessibility ,carotenoids ,metabolomics ,zeaxanthin dipalmita ,Chemical technology ,TP1-1185 - Abstract
Goji berry (Lycium barbarum L., LBL) is a good source of carotenoids, while the bioaccessibility of various types of LBL carotenoids has not been explored. In the study, eight carotenoids, three carotenoid esters and two carotenoid glycosylated derivatives were identified by a non−targeted metabolomics approach. The dried LBL (DRI), LBL in water (WAT), and LBL in “Baijiu” (WIN) were used to recreate the three regularly chosen types of utilization, and the in vitro digestion model showed that the bioaccessibility of the carotenoids increased significantly from the oral to the gastric and intestinal phase (p < 0.05). The bioaccessibility of LBL carotenoids was the most elevated for DRI (at 28.2%), followed by WIN and WAT (at 24.9% and 20.3%, respectively). Among the three carotenoids, zeaxanthin dipalmitate showed the highest bioaccessibility (51.8–57.1%), followed by β−carotene (51.1–55.6%) and zeaxanthin (45.2–56.3%). However, the zeaxanthin from DRI exhibited significantly higher bioaccessibility (up to 58.3%) than WAT and WIN in both the gastric and intestinal phases (p < 0.05). Results of antioxidant activity tests based on DPPH, FRAP, and ABTS showed that the addition of lipids improved the bioaccessibility of the carotenoids. (p < 0.05).
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- 2022
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17. N6-Methyladenosine RNA Modification: An Emerging Immunotherapeutic Approach to Turning Up Cold Tumors
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Lei Zhan, Junhui Zhang, Suding Zhu, Xiaojing Liu, Jing Zhang, Wenyan Wang, Yijun Fan, Shiying Sun, Bing Wei, and Yunxia Cao
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N6-methyladenosine RNA modification ,tumor microenvironment ,cold tumors ,hot tumors ,biomarker ,prognosis ,Biology (General) ,QH301-705.5 - Abstract
Immunotherapy is a novel clinical approach that has shown clinical efficacy in multiple cancers. However, only a fraction of patients respond well to immunotherapy. Immuno-oncological studies have identified the type of tumors that are sensitive to immunotherapy, the so-called hot tumors, while unresponsive tumors, known as “cold tumors,” have the potential to turn into hot ones. Therefore, the mechanisms underlying cold tumor formation must be elucidated, and efforts should be made to turn cold tumors into hot tumors. N6-methyladenosine (m6A) RNA modification affects the maturation and function of immune cells by controlling mRNA immunogenicity and innate immune components in the tumor microenvironment (TME), suggesting its predominant role in the development of tumors and its potential use as a target to improve cancer immunotherapy. In this review, we first describe the TME, cold and hot tumors, and m6A RNA modification. Then, we focus on the role of m6A RNA modification in cold tumor formation and regulation. Finally, we discuss the potential clinical implications and immunotherapeutic approaches of m6A RNA modification in cancer patients. In conclusion, m6A RNA modification is involved in cold tumor formation by regulating immunity, tumor-cell-intrinsic pathways, soluble inhibitory mediators in the TME, increasing metabolic competition, and affecting the tumor mutational burden. Furthermore, m6A RNA modification regulators may potentially be used as diagnostic and prognostic biomarkers for different types of cancer. In addition, targeting m6A RNA modification may sensitize cancers to immunotherapy, making it a promising immunotherapeutic approach for turning cold tumors into hot ones.
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- 2021
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18. NLRC5 Inhibits Inflammation of Secretory Phase Ectopic Endometrial Stromal Cells by Up-Regulating Autophagy in Ovarian Endometriosis
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Runhua He, Xiaojing Liu, Jing Zhang, Zhongzheng Wang, Wenyan Wang, Liutao Fu, Yijun Fan, Shiying Sun, Yunxia Cao, Lei Zhan, and Lijun Shui
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NLRC5 ,ovarian endometriosis ,ectopic endometrial stromal cells ,autophagy ,inflammation ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Nod-like receptor (NLR) family caspase activation and recruitment domain containing 5 (NLRC5) is a newly identified sub-class of the NLR family. It regulates inflammation and has a key function in innate and adaptive immunologic reactions. Autophagy has been reported to be crucially linked to the pathogenesis of endometriosis. Our recent study identify there is a negative correlation between NLRC5 and autophagy in endometriosis, indicating that NLRC5 and autophagy together act as promising predictors in endometriosis patients. However, the mechanism associating NLRC5 and autophagy in endometriosis is still not completely understood. We hypothesize that autophagy could be involved in NLRC5-mediated inflammation in endometriosis. In order to validate the assumption, we evaluate the effects of NLRC5 and autophagy in the inflammation of ectopic endometrial stromal cells (EESCs) of ovarian endometriosis patients, to specifically determine whether autophagy is involved in NLRC5-mediated inflammation in EESCs. Our results show that over-expression of NLRC5 results in the up-regulation of autophagy in EESCs and inhibition of NLRC5 restricts the level of autophagy in EESCs. Furthermore, over-expression of NLRC5 and promotion of autophagy inhibit interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) expressions, whereas inhibition of NLRC5 and autophagy up-regulate IL-6 and TNF-α expressions in EESCs. Additionally, promotion of autophagy contributes to the NLRC5-mediated inhibition of IL-6 and TNF-α expressions in EESCs; inhibition of autophagy restricts NLRC5-mediated inhibition of IL-6 and TNF-α expressions in EESCs. Our results suggest that over-expression of NLRC5 promotes autophagy, thereby inhibiting inflammation in ovarian endometriosis.
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- 2020
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19. NLRC5 promotes cell migration and invasion by activating the PI3K/AKT signaling pathway in endometrial cancer
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Yijun Fan, Zhen Dong, Yuchuan Shi, Shiying Sun, Bing Wei, and Lei Zhan
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Medicine (General) ,R5-920 - Abstract
Objective NOD-like receptor family caspase recruitment domain family domain-containing 5 (NLRC5) is involved in the development of cancer. Our objective was to explore the role of NLRC5 in the progression of endometrial cancer (EC). Methods The roles of NLRC5 in migration and invasion of AN3CA EC cells were examined by cell wound-healing assay, Transwell migration, and invasion analysis. Overexpression of NLRC5 was achieved with NLRC5 plasmid, and knockdown of NLRC5 was achieved using small interfering (si)RNA-NLRC5 in AN3CA cells. The expression of NLRC5 was detected by immunohistochemical, western blot, and quantitative real-time PCR. LY294002 was used to inhibit the phosphatidylinositol 3-kinase (PI3K)/AKT signaling pathway. Results NLRC5 was downregulated in EC tissue compared with normal endometrium. Overexpression of NLRC5 led to upregulation of cell migration and invasion in AN3CA cells and expression of matrix metallopeptidase (MMP)-9. Inhibition of NLRC5 restricted migration and invasion of AN3CA cells and expression of MMP9. Overexpression of NLRC5 promoted the activation of PI3K/AKT signaling pathway. Inhibiting PI3K/AKT signaling pathway by using LY294002 blocked the positive role of NLRC5 in migration and invasion of AN3CA cells and expression of MMP9. Conclusions These results demonstrate that NLRC5 promotes EC progression by activating the PI3K/AKT signaling pathway.
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- 2020
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20. Characterization and Antihyperglycemic Activity of a Polysaccharide from Dioscorea opposita Thunb Roots
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Yijun Fan, Qinyi He, Aoshuang Luo, Miaoyu Wang, and Aoxue Luo
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Dioscorea opposita Thunb ,antihyperglycemic ,polysaccharide ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
A polysaccharide DOTP-80 from Dioscorea opposita Thunb was obtained by using the method of acid water-extraction and ethanol-precipitation. After being purified by chromatography, the structure characteristics of DOTP-80 were established. Based on the calibration curve obtained with standard dextrans, the molecular weight of the polysaccharide fraction DOTP-80 was calculated to be 123 kDa. The results of Infrared spectrum (FT-IR) indicated that the polysaccharide contained the α-configuration of sugar units. GC-MS analysis revealed that DOTP-80 was mainly composed of mannose and glucose. Alloxan-induced diabetic rats and mice models were developed to evaluate the in vivo hypoglycemic activity of the polysaccharide. The results indicated that a high dose DOTP-80 (400 mg/kg) had strong hypoglycemic activity. Moreover, DOTP-80 could increase the level of antioxidant enzymes (SOD) activity in alloxan-induced diabetic mice and stimulate an increase in glucose disposal in diabetic rats. Therefore, the polysaccharide DOTP-80 should be evaluated as a candidate for future studies on diabetes mellitus.
- Published
- 2015
- Full Text
- View/download PDF
21. Characterization and Antitumor Activity of a Polysaccharide from Sarcodia ceylonensis
- Author
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Yijun Fan, Mengchuan Lin, Aoshuang Luo, Ze Chun, and Aoxue Luo
- Subjects
Sarcodia ceylonensis ,polysaccharide ,antitumor ,purification ,Organic chemistry ,QD241-441 - Abstract
A water-soluble polysaccharide from Sarcodia ceylonensis was obtained by using the method of water-extraction and ethanol-precipitation. The polysaccharide was further purified by chromatography on AB-8 and ADS-7 columns, yielding a pure polysaccharide termed SCP-60. The molecular weight (Mw) of SCP-60 was calculated to be 50.0 kDa, based on the calibration curve obtained with a series of Dextran T standards. The results of FT-IR indicated that the polysaccharide contains the α-configuration of sugar units. GC-MS analysis revealed that SCP-60 was mainly composed of galactose and glucose. NMR spectroscopy revealed SCP-60 had the backbone consisting of →6)-α-Manp-(1→, α-d-Glcp-(1→, →6)-α-d-Glcp-(1→ and →6)-α-Galp-(1→. In order to evaluate the antitumor activity in vivo of the polysaccharide, a sarcoma 180 model was used. The results showed SCP-60 had strong antitumor ability, meanwhile, SCP-60 at a high dose (100 mg/kg) could significantly increase the thymic and splenic indices of S180 mice, and strongly promote the secretion of IL-2, TNF-α and IFN-γ, increase the SOD activities and reduce the concentrations of MDA in blood. Therefore the polysaccharide SCP-60 should be explored as a novel potential antitumor drug.
- Published
- 2014
- Full Text
- View/download PDF
22. Purification, Characterization and Antioxidant Activities in Vitro and in Vivo of the Polysaccharides from Boletus edulis Bull
- Author
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Yijun Fan, Jiandong Huang, Aoshuang Luo, and Aoxue Luo
- Subjects
Boletus edulis Bull ,polysaccharide ,purification ,antioxidant activity ,Organic chemistry ,QD241-441 - Abstract
A water-soluble polysaccharide (BEBP) was extracted from Boletus edulis Bull using hot water extraction followed by ethanol precipitation. The polysaccharide BEBP was further purified by chromatography on a DEAE-cellulose column, giving three major polysaccharide fractions termed BEBP-1, BEBP-2 and BEBP-3. In the next experiment, the average molecular weight (Mw), IR and monosaccharide compositional analysis of the three polysaccharide fractions were determined. The evaluation of antioxidant activities both in vitro and in vivo suggested that BEBP-3 had good potential antioxidant activity, and should be explored as a novel potential antioxidant.
- Published
- 2012
- Full Text
- View/download PDF
23. In vitro Antioxidant of a Water-Soluble Polysaccharide from Dendrobium fimhriatum Hook.var.oculatum Hook
- Author
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Yijun Fan and Aoxue Luo
- Subjects
Dendrobium fimhriatum Hook.var.oculatum Hook ,polysaccharide ,antioxidant activity ,in vitro ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
A water-soluble crude polysaccharide (DFHP) obtained from the aqueous extracts of the stem of Dendrobium fimhriatum Hook.var.oculatum Hook through hot water extraction followed by ethanol precipitation, was found to have an average molecular weight (Mw) of about 209.3 kDa. Monosaccharide analysis revealed that DFHP was composed of mannose, glucose and galactose in a content ratio of 37.52%; 43.16%; 19.32%. The investigation of antioxidant activity in vitro showed that DFHP is a potential antioxidant.
- Published
- 2011
- Full Text
- View/download PDF
24. In Vitro and In Vivo Antioxidant Activity of a Water-Soluble Polysaccharide from Dendrobium denneanum
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XingJin He, Yijun Fan, Aoshuang Luo, Aoxue Luo, Ze Chun, and Zhongfu Ge
- Subjects
Dendrobium denneanum ,polysaccharide ,antioxidant activity ,Organic chemistry ,QD241-441 - Abstract
The water-soluble crude polysaccharide (DDP) obtained from the aqueous extracts of the stem of Dendrobium denneanum through hot water extraction followed by ethanol precipitation, was found to have an average molecular weight (Mw) of about 484.7 kDa. Monosaccharide analysis revealed that DDP was composed of arabinose, xylose, mannose, glucose and galactose in a molar ratio of 1.00:2.66:8.92:34.20:10.16. The investigation of antioxidant activity both in vitro and in vivo showed that DDP is a potential antioxidant.
- Published
- 2011
- Full Text
- View/download PDF
25. Chlamydial lung infection induces transient IL-9 production which is redundant for host defense against primary infection.
- Author
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Ying Peng, Xiaoling Gao, Jie Yang, Sudhanshu Shekhar, Shuhe Wang, Yijun Fan, and Xi Yang
- Subjects
Medicine ,Science - Abstract
IL-9/Th9 responses are recently found to be important for innate and adaptive immunity particularly in parasitic infections. To date, the study on the role of IL-9 in bacterial infections is limited and the reported data are contradictory. One reported function of IL-9/Th9 is to modulate Th1/Th17 responses. Since our and others' previous work has shown a critical role of Th1 and Th17 cells in host defense against chlamydial lung infection, we here examined the role of IL-9 responses in Chlamydia muridarum (Cm) lung infection, particularly its effect on Th1 and Th17 responses and outcome infection. Our data showed quick but transient IL-9 production in the lung following infection, peaking at day 3 and back to baseline around day 7. CD4+ T cell was the major source of IL-9 production in the lung infection. Blockade of endogenous IL-9 using neutralizing antibody failed to change Interferon-γ (IFN-γ) and IL-17 production by cultured spleen mononuclear cells isolated from Cm infected mice. Similarly, in vivo neutralization of IL-9 failed to show significant effect on T cell (Th1 and Th17) and antibody responses (IgA, IgG1 and IgG2a). Consistently, the neutralization of IL-9 had no significant effect on disease process, including body weight change, bacterial burden and histopathological score. The data suggest that IL-9 production following chlamydial lung infection is redundant for host defense against the intracellular bacteria.
- Published
- 2015
- Full Text
- View/download PDF
26. X-Oscar: A Progressive Framework for High-quality Text-guided 3D Animatable Avatar Generation.
- Author
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Yiwei Ma, Zhekai Lin, Jiayi Ji, Yijun Fan, Xiaoshuai Sun, and Rongrong Ji
- Published
- 2024
27. CD8+ DC, but Not CD8(-)DC, isolated from BCG-infected mice reduces pathological reactions induced by mycobacterial challenge infection.
- Author
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Xiaoling Gao, Shuhe Wang, Yijun Fan, Hong Bai, Jie Yang, and Xi Yang
- Subjects
Medicine ,Science - Abstract
Tuberculosis is a mycobacterial infection causing worldwide public health problems but the available vaccine is far from ideal. Type-1 T cell immunity has been shown to be critical for host defence against tuberculosis infection, but the role of dendritic cell (DC) subsets in pathogenesis of mycobacterial infection remains unclear.We examined the effectiveness of dendritic cell (DC) subsets in BCG-infected mice in generating immune responses beneficial for pathogen clearance and reduction of pathological reactions in the tissues following challenge infection. Our data showed that only the adoptive transfer of the subset of CD8alpha+ DC isolated from infected mice (iCD8+ DC) generated significant protection, demonstrated by less mycobacterial growth and pathological changes in the lung and liver tissues in iCD8+ DC recipients than sham-treated control mice. The adoptive transfer of the CD8alpha(-)DC from the infected mice (iCD8(-) DC) not only failed to reduce bacterial growth, but enhanced inflammation characterized by diffuse heavy cellular infiltration. Notably, iCD8(-) DC produced significantly higher levels of IL-10 than iCD8+ DC and promoted more Th2 cytokine responses in in vitro DC-T cell co-culture and in vivo adoptive transfer experiments.The data indicate that in vivo BCG-primed CD8+ DC is the dominant DC subset in inducing protective immunity especially for reducing pathological reactions in infected tissues. The finding has implications for the rational improvement of the prophylactic and therapeutic approaches for controlling tuberculosis infection and related diseases.
- Published
- 2010
- Full Text
- View/download PDF
28. Dependence Analysis and Structured Construction for Batched Sparse Code.
- Author
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Jiaxin Qing, Xiaohong Cai, Yijun Fan, Mingyang Zhu, and Raymond W. Yeung
- Published
- 2024
- Full Text
- View/download PDF
29. Reliable Throughput of Generalized Collision Channel without Synchronization.
- Author
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Yijun Fan, Yanxiao Liu 0003, Yi Chen 0013, Shenghao Yang 0001, and Raymond W. Yeung
- Published
- 2023
- Full Text
- View/download PDF
30. Continuity of Link Scheduling Rate Region for Wireless Networks with Propagation Delays.
- Author
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Yijun Fan, Yanxiao Liu 0003, and Shenghao Yang 0001
- Published
- 2022
- Full Text
- View/download PDF
31. X-Dreamer: Creating High-quality 3D Content by Bridging the Domain Gap Between Text-to-2D and Text-to-3D Generation.
- Author
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Yiwei Ma, Yijun Fan, Jiayi Ji, Haowei Wang 0001, Xiaoshuai Sun, Guannan Jiang, Annan Shu, and Rongrong Ji
- Published
- 2023
- Full Text
- View/download PDF
32. PDF report automatic generation system based on JAVA.
- Author
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Yijun Fan, Guangyan Lin, and Huobin Tan
- Published
- 2018
- Full Text
- View/download PDF
33. Research on the Effectiveness of Integrating Ideological and Political Education into Higher Mathematics Teaching
- Author
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Linli Dou, Yijun Fan, and Shanshan Yu
- Published
- 2022
34. Transcriptome Analysis Revealed the Effects of Different Levels of Trace Elements(B, Mo, Zn) on the Physiology and Medicinal Ingredients of Dendrobium Nobile
- Author
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shengdi wu, Aoxue Luo, Gang Wang, Kaigang Yang, Erya Xu, Yuanyuan Liu, Xuebing Li, Jie Ma, and Yijun Fan
- Published
- 2023
35. Pharmacokinetics, safety and bioequivalence of two formulations of progesterone soft capsule in healthy Chinese postmenopausal females: Impacts of a high‐fat meal
- Author
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Wei Hu, Anding Liu, Huiling Qin, Yuanyuan Fang, Jing Chen, Fengjia Zhu, Yijun Fan, Yueyue Liu, Jinlian Wu, and Liang Zheng
- Subjects
media_common.quotation_subject ,Cmax ,Physiology ,Bioequivalence ,Toxicology ,Cohort Studies ,Food-Drug Interactions ,Asian People ,Pharmacokinetics ,Humans ,Medicine ,Adverse effect ,Ovulation ,Progesterone ,media_common ,Pharmacology ,Meal ,business.industry ,Area under the curve ,Fasting ,General Medicine ,Middle Aged ,Dietary Fats ,Postmenopause ,Therapeutic Equivalency ,Area Under Curve ,Cohort ,Female ,Progestins ,business ,Half-Life - Abstract
Progesterone is an important natural hormone regulating ovulation and menstruation. The present study aimed to investigate the pharmacokinetics and safety of two formulations of progesterone in Chinese postmenopausal females under fasting and fed conditions. The study adopted a single-dose, open-label, randomized, three-period bioequivalence design. A total of 96 subjects were enrolled and randomly assigned to the fasting cohort or fed cohort. A high-fat meal (890 kcal) was used in the fed study. The reference-scaled average bioequivalence method was used for bioequivalence evaluation. A high-fat meal led to a 22-fold higher peak concentration (Cmax ) and a 7-fold higher area under the curve (AUC) while time to reach Cmax and half-life was not significantly affected. The concentration-time curve displayed double peaks suggesting the existence of enterohepatic circulation. The test/reference geometric mean ratios for Cmax and AUC under fasting and fed conditions are all within the range of 80% to 125%. All adverse events (AEs) that occurred during the trial were mild and did not cause drop-out, though these AEs occurred more frequently under fed state. In conclusion, the two formulations of progesterone are bioequivalent in Chinese subjects under fasting and fed conditions. Drug label modification regarding food effects needs further discussion.
- Published
- 2021
36. Extraction, characterization and antioxidant activities of an acidic polysaccharide from Dendrobium devonianum
- Author
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Aoxue Luo, Mengchuan Lin, and Yijun Fan
- Subjects
chemistry.chemical_classification ,Antioxidant ,ABTS ,biology ,General Chemical Engineering ,medicine.medical_treatment ,Radical ,Polysaccharide ,biology.organism_classification ,Industrial and Manufacturing Engineering ,chemistry.chemical_compound ,chemistry ,Dendrobium devonianum ,medicine ,Monosaccharide ,Hydroxyl radical ,Food science ,Safety, Risk, Reliability and Quality ,Sugar ,Food Science - Abstract
Dendrobium devonianum Paxt (D. devonianum) is widely used as a healthy food and traditional Chinese medicine. It is rich in bioactive polysaccharides, but the acidic polysaccharide have not been reported. In this research, the enzyme-assisted extraction and structure characterizations of an acid polysaccharide (DDPp2) and its antioxidant activities were studied. The results showed the optimum extraction conditions were at enzyme concentration of 0.94% at 51.06 °C and pH 4.94. The molecular weight of DDPp2 was 306 kDa and composed of four monosaccharides in the molar ratio of mannose:glucose:galactose:glucuronic acid = 6.37:4.99:1.71:2.39 with α-configuration sugar. SEM and TEM analysis showed that the surface of the polysaccharide was smooth and holes in the sheet and multiple molecular chains were intertwined with each other. Free radical scavenging tests in vitro showed DDPp2 had very strong scavenging abilities for hydroxyl radical, ABTS radical and superoxide anion free radicals. Cell experiment showed DDPp2 had no cytotoxicity and could significantly increase the activities of CAT, SOD and GPX, reduce the content of MDA in RAW264.7 cells. The investigation showed that DDPp2 is a potential antioxidant.
- Published
- 2021
37. Intuitionistic fuzzy random variables.
- Author
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Chao Wang 0034, Minghu Ha 0001, Yijun Fan, and Jiqiang Chen
- Published
- 2011
- Full Text
- View/download PDF
38. Cerium improves the physiology and medicinal components of Dendrobium nobile Lindl. under copper stress
- Author
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Xuebing Li, Yijun Fan, Jie Ma, Xiaoya Gao, Gang Wang, Shengdi Wu, Yuanyuan Liu, Kaigang Yang, Erya Xu, Shangrao Pu, and Aoxue Luo
- Subjects
Physiology ,Plant Science ,Agronomy and Crop Science - Abstract
Heavy metal stress affects the quality of medicinal plants, and rare earth elements can effectively alleviate heavy metal stress. In this paper, we investigated the effects of rare earth element cerium (0, 5, 10, 20, 40, 80, and 160 mg/L) on the physiological and medicinal components of Dendrobium nobile Lindl. under copper (200 mg/L) stress. The results revealed that cerium (Ce) had a good alleviating effect on copper (Cu) stress, low concentrations of Ce (10-20 mg/L) significantly improved the resistance and medicinal qualities of the plant such as polysaccharide, polyphenol and flavonoid, it also increased the content of photosynthetic pigment, proline, soluble sugar and soluble protein of D. nobile Lindl., effectively balance the osmotic pressure and the generation and removal of reactive oxygen species in the plant, thereby the toxic effect of copper on D. nobile Lindl. is alleviated. From the point of view of the treatment time when the optimal relieving concentration appeared, the optimal concentration for relieving antioxidant enzyme activity all appeared at the treatment time of 10 d, the optimum concentrations of other indicators all appeared at the treatment time of 15 d. Overall, this study suggests that the optimum level of Ce (10-20 mg/L) might be promising for alleviating the adverse impacts of copper stress and promoting the accumulation of medicinal components in D. nobile Lindl.
- Published
- 2022
39. Androgen Receptor Is Expressed in the Majority of Breast Cancer Brain Metastases and Is Subtype-Dependent
- Author
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Kevin Yijun Fan, Rania Chehade, Maleeha Qazi, Veronika Moravan, Sharon Nofech-Mozes, and Katarzyna J. Jerzak
- Subjects
androgen receptor ,breast cancer ,brain metastasis ,immunohistochemistry ,Cancer Research ,Oncology - Abstract
We aimed to evaluate the expression of the “targetable” androgen receptor (AR) in breast cancer brain metastases (BrM). An established, retrospective 57-patient cohort with metastatic breast cancer who underwent surgery for BrM at the Sunnybrook Odette Cancer Centre between 1999–2013 was studied. AR expression in BrM samples was assessed in triplicate using immunohistochemistry (IHC). AR positive status was defined as nuclear AR expression ≥ 10% by IHC using the SP107 antibody. The median age of patients was 52 years (range 32–85 years). 28 (49%) of BrM were HER2+, 17 (30%) were hormone receptor positive (HR+)/HER2−, and 12 (21%) were triple negative breast cancers (TNBCs). 56% (n = 32/57) of BrM were AR positive, and median AR expression was 20% (CI 1.6–38.3%). AR expression was different across breast cancer subtypes; AR was most frequently expressed in HER2+ (n = 21/28), followed by HR+/HER2− (n = 9/17), and lowest in TNBC (n = 2/12) BrM (p = 0.003). Patients with AR positive versus AR negative BrM had similar overall survival (12.5 vs. 7.9 months, p = 0.6), brain-specific progression-free survival (8.0 vs. 5.1 months, p = 0.95), and time from breast cancer diagnosis to BrM diagnosis (51 vs. 29 months, p = 0.16). AR is expressed in the majority of breast cancer BrM and represents a potential therapeutic target.
- Published
- 2023
40. Thevenin Theorem and Norton's Theorem
- Author
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Yijun Fan, Yunpeng Hu, and Jihong Li
- Published
- 2022
41. A Development of Inclusion-degree-based Rough Fuzzy Random Sets.
- Author
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Minghu Ha 0001, Witold Pedrycz, Aiquan Zhang, and Yijun Fan
- Published
- 2008
42. Analysis of platinum resistance temperature sensor circuit based on CDIO
- Author
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Yunpeng Hu, Yijun Fan, and Miao Zhang
- Published
- 2021
43. Research on Transistor Amplifier Circuit Based on Project
- Author
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Jihong Li, Miao Zhang, and Yijun Fan
- Published
- 2021
44. LC3 and NLRC5 interaction inhibits NLRC5-mediated MHC class I antigen presentation pathway in endometrial cancer
- Author
-
Lei Zhan, Junhui Zhang, Jing Zhang, Xiaojing Liu, Suding Zhu, Yuchuan Shi, Yu He, Wenyan Wang, Yijun Fan, Zhenhai Tang, Guo Chen, Bing Wei, and Yunxia Cao
- Subjects
Cancer Research ,Antigen Presentation ,Histocompatibility Antigens Class I ,Intracellular Signaling Peptides and Proteins ,Middle Aged ,Models, Biological ,Xenograft Model Antitumor Assays ,Autophagic Punctum ,Endometrial Neoplasms ,Immunophenotyping ,Gene Expression Regulation, Neoplastic ,Disease Models, Animal ,Mice ,Oncology ,Cell Line, Tumor ,Animals ,Humans ,Female ,Neoplasm Grading ,Microtubule-Associated Proteins ,Aged ,Neoplasm Staging ,Signal Transduction - Abstract
Background: The major histocompatibility complex class I (MHC- I) transactivator, nucleotide binding oligomerization domain-like receptor family caspase recruitment domain containing 5 (NLRC5), serves as a target for immune evasion in many cancers, including endometrial cancer (EC). An inhibition of autophagy can contribute to immunotherapy by assisting the MHC-I-mediated antigen presentation in cancer. However, the underlying mechanism for autophagy-regulated MHC-I in EC remains unclear. Our study aimed to investigate the effect of autophagy on NLRC5 and MHC-I-mediated antigen presentation, and to identify the potential mechanisms underlying this process in EC.Methods: We examined the levels of autophagy and MHC-I genes by performing transmission electron microscopy (TEM), RNA-seq sequencing, western blotting, and qRT-PCR. The t-test, F-test, Kaplan-Meier analysis, and Pearson’s correlation analysis were used for statistical evaluations of tissue microarrays. Immunofluorescence staining, co-immunoprecipitation (CO-IP), and glutathione S-transferase (GST) pull-down assay were performed. HEC-1A, AN3CA, and Ishikawa EC cells were transfected designed, and the role of LC3 and NLRC5 in MHC-I-mediated antigen presentation in EC was further evaluated in a xenotransplantation model of HEC-1A cell line. Results: Autophagy was upregulated in EC endometrium as compared to that in normal endometrium. MHC I and NLRC5 expressions were lower in EC endometrium than in normal endometrium. Autophagy played a negative role in the MHC-I genes expression in vitro. Furthermore, a negative correlation was found between LC3 and NLRC5 levels, and LC3 interacted with NLRC5 to inhibit NLRC5-mediated MHC-I antigen presentation pathway in vitro and in vivo. Conclusion: An upregulation of LC3 in EC patients may contribute to tumor immune escape by restricting the NLRC5-mediated MHC-I antigen presentation pathway, suggesting that inhibiting LC3 and promoting NLRC5 may be a promising immunotherapy strategy in the management of EC.
- Published
- 2021
45. Population Pharmacokinetic and Exposure-Response Model Simulations: Predicted Exposure and Efficacy for Maintenance Doses of Intravenous Golimumab Every 6 or 8 Weeks in Patients With Moderately to Severely Active Rheumatoid Arthritis
- Author
-
Jong Bong Lee, Aaron Broadwell, Yijun Fan, Chuanpu Hu, Omoniyi J. Adedokun, Soumya D. Chakravarty, Honghui Zhou, Zhenhua Xu, and Jocelyn H. Leu
- Subjects
Pharmacology ,Arthritis, Rheumatoid ,Methotrexate ,Treatment Outcome ,Antirheumatic Agents ,Antibodies, Monoclonal ,Humans ,Pharmacology (medical) ,Drug Therapy, Combination - Abstract
Golimumab is approved to treat moderate-to-severe active rheumatoid arthritis when given intravenously at weeks 0 and 4, then every 8 weeks (Q8W) with concomitant methotrexate. These analyses assessed whether a shorter dosing interval could ameliorate diminished efficacy experienced by a small proportion of patients toward the end of the dosing interval.Population pharmacokinetic and exposure-response modeling simulations were performed for intravenous golimumab 2 mg/kg at weeks 0 and 4, then Q8W or every 6 weeks (Q6W) through 1 year. A 2-compartment pharmacokinetic model with linear clearance developed based on GO-FURTHER (A Multicenter, Randomized, Double-blind, Placebo-controlled Trial of Golimumab, an Anti-TNFα Monoclonal Antibody, Administered Intravenously, in Patients With Active Rheumatoid Arthritis Despite Methotrexate Therapy) study data was used for pharmacokinetic simulations. A latent-variable indirect exposure-response model developed based on GO-FURTHER American College of Rheumatology (ACR) 20%/50%/70% improvement (ACR20, ACR50, and ACR70, respectively) data was used to predict clinical endpoints of ACR20/ACR50/ACR70 response rates.For Q6W and Q8W dosing, respectively, predicted median golimumab steady-state trough (CThese simulations suggest that intravenous golimumab Q6W dosing increases golimumab Cgov identifier: NCT00973479. Clinicaltrialsregister.eu: EudraCT 2008-006064-11.
- Published
- 2021
46. Efficacy and Safety of Bone-Targeting Radioisotopes in Patients with Bone Metastases: A Meta-Analysis
- Author
-
Yijun Fan, Guoping Shen, and Wanzhong Ye
- Subjects
Oncology ,medicine.medical_specialty ,Bone targeting ,business.industry ,Meta-analysis ,Internal medicine ,Medicine ,In patient ,business - Abstract
Background: Radionuclides such as Strontium-89, Samarium-153, and Radium-223 are commonly used for the treatment of bone metastases (BM) and they have shown efficacy in previous studies. The aim of this meta-analysis is to compare the curative performance of different types of radionuclides in patients with BM, and to provide evidence for further future research and clinical practice.Methods: The electronic databases of PubMed, Web of Science, Embase, Cochrane Library, Wanfang Data, and China National Knowledge Infrastructure (CNKI) were searched. Studies using radionuclides therapy to cure patients were included. Pooled overall response rate, occurrence rates of common adverse events, hazard ratio (HR), and their respective 95% confidence interval (CIs) were calculated.Results: A total of 28 articles with 33 studies patients were identified for inclusion in this meta-analysis. The types of radionuclides used in the studies contained 89Sr, 153Sm, 223Ra, 186Re, and 188Re. The overall response rate was 72% [66%, 77%]. The respective overall response rates of 89Sr, 153Sm, and 223Ra were 72% [64%, 79%], 80% [75%, 84%], and 54% [32%, 75%]. In the survival analysis, the pooled HR was 0.85 [0.67, 1.08]. The pooled occurrence rate of leucocyte hematological toxicity of any grade was 5% [2%, 8%]. The pooled occurrence rate of thrombocytopenia of any grade was 7% [5%, 8%]. With regard to anemia, the pooled occurrence rate of any grade was 15% [11%, 19%].Conclusion: The results of this meta-analysis indicate that radionuclides therapy is efficacious in patients, type of radionuclides and history of patients should be considered to prevent potential adverse events.
- Published
- 2021
47. Simulation and Application of Single Tube Amplifier Circuit
- Author
-
Yijun Fan, Miao Zhang, and Yunpeng Hu
- Subjects
Physics ,Operating point ,business.industry ,Amplifier ,Transistor ,Electrical engineering ,Hardware_PERFORMANCEANDRELIABILITY ,law.invention ,Single tube ,Computer Science::Hardware Architecture ,Computer Science::Emerging Technologies ,Hardware_GENERAL ,law ,Nonlinear distortion ,Distortion ,Hardware_INTEGRATEDCIRCUITS ,business ,Energy (signal processing) ,Hardware_LOGICDESIGN ,Voltage - Abstract
The transistor is the core component of the amplifying circuit. It can control the conversion of energy and amplify any small changes in the input without distortion. In the Multisim simulation environment, this article verifies the static operating point, voltage amplification and other performance parameters of the single-tube amplifier circuit according to the given circuit, and observes the nonlinear distortion phenomenon of the amplifier circuit.
- Published
- 2021
48. Simulation of Low Voltage Electrical System of Tram
- Author
-
Jihong Li, Yijun Fan, and Yunpeng Hu
- Subjects
Generator (circuit theory) ,Battery (electricity) ,Electric power system ,Hardware_GENERAL ,business.industry ,Computer science ,Electrical equipment ,Circuit design ,Electrical engineering ,Idle speed ,business ,Low voltage ,Power (physics) - Abstract
Automotive electrical equipment can be summarized into three major components: power supply system, electrical equipment, and intermediate devices. For any electrical equipment and electronic control device to obtain power supply, the connection of intermediate devices is essential. The generator is the main power source of the car, and its function is to supply power to all electrical equipment (except the starter) when the engine is running normally (above idle speed), and to charge the battery at the same time. In this paper, by modeling the circuit of low-voltage electrical system of trams and designing and realizing the circuit model of low-voltage electrical system, this article masters the basic knowledge of circuit analysis, is familiar with the general process of circuit design, and can solve the general circuit faults in debugging.
- Published
- 2021
49. Transcriptome analysis reveals the effects of red and blue light on the physiological and medicinal components of Dendrobium denneanum
- Author
-
Yijun Fan, Kaigang Yang, Rongsu Miao, Gang Wang, Ze Chun, Shengdi Wu, Shangrao Pu, and Aoxue Luo
- Subjects
Agronomy and Crop Science - Published
- 2022
50. Zinc affects the physiology and medicinal components of Dendrobium nobile Lindl
- Author
-
Gang Wang, Jiang Ting, Tan Xueyan, Zhao Juan, Aoxue Luo, Shangrao Pu, Ze Chun, Yijun Fan, and Yang Kaigang
- Subjects
0106 biological sciences ,0301 basic medicine ,Stomatal conductance ,Physiology ,chemistry.chemical_element ,Plant Science ,Zinc ,Polysaccharide ,Photosynthesis ,01 natural sciences ,Dendrobium nobile ,Superoxide dismutase ,03 medical and health sciences ,Polysaccharides ,Genetics ,Food science ,chemistry.chemical_classification ,biology ,biology.organism_classification ,Ascorbic acid ,Plant Leaves ,030104 developmental biology ,chemistry ,Polyphenol ,biology.protein ,Dendrobium ,010606 plant biology & botany - Abstract
The growth of Dendrobium nobile is often influenced by zinc. Here, D. nobile was regularly sprayed with different concentrations (0, 50, 100, 200, 400, 800, 1000, 2000 μmol L−1) of zinc to study its effect on the growth and biosynthesis of medicinal components. Samples were taken at 0, 7, 14, and 21 days to detect physiological and medicinal components. The results showed that the net photosynthetic rate, transpiration rate, stomatal conductance, and Chl A and Chl B levels of leaves first increased and then decreased as the concentration of zinc increased. At 400 μmol L−1 concentration, these parameters reached their maximum values. Thus, a certain dose of zinc could promote the photosynthesis of D. nobile. There was an obvious increase in the synthesis of superoxide dismutase (SOD), while the content of ascorbate peroxidase and ascorbic acid (AsA) were the highest after treatment with 400 μmol L−1 zinc. Maximum levels of polysaccharides and polyphenols were observed on day 7 and 14, respectively, at a zinc concentration of 400 μmol L−1. These results suggest that exogenous zinc may promote the accumulation of medicinal components in D. nobile. It was also found that polysaccharides could combine well with zinc to form a polysaccharide-zinc chelate and transform inorganic zinc into organic form, which is stored in the form of polysaccharide-Zn and is known to reduce the damage induced by Zn stress.
- Published
- 2020
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