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Antennas are important components in optical phased arrays. However, their far-field performance deteriorates when random phase noise is introduced because of fabricating errors. For the first time, we use a finite-difference time-domain solution to quantitatively analyze the far-field characteristics of Si and Si3N4 antennas considering process errors. Under rough surface conditions based on a fishbone structure, we find that the quality of the main lobe of the Si antenna deteriorates badly, with −0.87 dB and −0.51 dB decreases in the sidelobe level and 5.78% and 3.74% deteriorations in the main peak power in the φ (phase-controlled) and θ (wavelength-controlled) directions, respectively. However, the Si3N4 antenna is only slightly impacted, with mere 0.39% and 0.71% deteriorations in the main peak power in the φ and θ directions, respectively, which is statistically about 1/15 of the Si antenna in the φ direction and 1/5 in the θ direction. The decreases in the sidelobe level are also slight, at about −0.08 dB and −0.01 dB, respectively. Furthermore, the advantages of the Si3N4 antenna become more remarkable with the introduction of random errors into the waveguide width and thickness. This work is of great significance for the design and optimization of OPA chips.

A numerical simulation on the heat transport augmentation and flow drag behavior of spirally corrugated pipes was performed. The simulation was conducted on the basis of the experimental results documented in the published literature. The influence of the thread height and pitch on the hydraulic–thermal performance as well as the mechanism of the convection heat transport development inside the spirally corrugated pipe were explored. It was discovered that the convection heat transport performance elevates in the Reynolds number region of 4000~13,000 as the thread height rises or the Reynolds number enlarges, but it declines when the thread pitch extends. The convection heat transport performance marked by the Nusselt number of the spirally corrugated pipe could reach 2.77 times that of the plain pipe, while the flow resistance coefficients of spirally corrugated pipes are 89~324% above that of the plain pipe. It enlarges with the rise in thread height but declines with the extension of the thread pitch. It also reduces when the Reynolds number enlarges. The factors of overall heat transmission performance for all the spirally corrugated pipes are above 1.00, and they increase in the Reynolds number region of 4000~7000 and then decrease in the Reynolds number region of 7000 to 13,000. The secondary flow at the cross-sections and the vortex between two adjacent corrugated grooves are the basic causes of the promotion of convection heat transport inside the spirally corrugated pipes. The secondary flow near the pipe wall both disrupts the border layer and boosts the radial interfusion of the fluid. In addition, the existence of vortexes makes the secondary flow act on the convection heat transmission continuously and positively in the region close to the pipe wall.

Sorghum faces significant production challenges due to drought stress. Melatonin has been demonstrated to play a crucial role in coping with stresses in plants. This study investigated the effect of exogenous melatonin on the sorghum seedling growth, photosynthetic capacity, and antioxidant system under drought stress. The results indicated that drought stress inhibited the growth of sorghum seedlings by a marked reduction in leaf relative water content, along with a significant increase in both malondialdehyde and hydrogen peroxide content. The drought stress also led to a significant diminution in chlorophyll contents, thereby curtailing the capacity for light energy capture. Furthermore, the efficiency of the photosynthetic electron transport chain was adversely impacted. However, the application of exogenous melatonin notably mitigated the adverse effects on sorghum seedlings under the drought stress. Additionally, it stimulated an elevation in the photosynthetic rate and a decrease in non-photochemical quenching. The exogenous melatonin also facilitated the preservation of the chloroplast ultra-structure and boosted the activity of antioxidant enzymes and the content of non-enzymatic antioxidants. Cluster heat maps and principal component analysis further revealed significant correlations among various parameters under different treatment conditions. These results highlight melatonin’s role in improving sorghum’s drought tolerance, which is beneficial for agricultural management.

Lassa virus (LASV), a risk-group 4 pathogen, must be handled in biosafety level-4 (BSL-4) conditions, thereby limiting its research and antiviral development. Here, we developed a novel LASV reverse genetics system which, to our knowledge, is the first to study the complete LASV life cycle under BSL-2 conditions. Viral particles can be produced efficiently when LASV minigenomic RNA harbouring minimal viral cis-elements and reporter genes is transfected into a helper cell line stably expressing viral NP, GP, Z and L proteins. The resulting defective virions, named LASVmg, can propagate only in the helper cell line, providing a BSL-2 model to study the complete LASV life cycle. Using this model, we found that a previously reported cellular receptor α-dystroglycan is dispensable for LASVmg infection. Furthermore, we showed that ribavirin can inhibit LASVmg infection by inducing viral mutations. This new BSL-2 system should facilitate studying the LASV life cycle and screening antivirals.

Tactile sensing has become indispensable for contact-rich dynamic robotic manipulation tasks. It provides robots with a better understanding of the physical environment, which is a vital supplement to robotic vision perception. Compared with other existing tactile sensors, vision-based tactile sensors (VBTSs) stand out for augmenting the tactile perception capabilities of robotic systems, owing to superior spatial resolution and cost-effectiveness. Despite their advantages, VBTS production faces challenges due to the lack of standardised manufacturing techniques and heavy reliance on manual labour. This limitation impedes scalability and widespread adoption. This paper introduces a rapid monolithic manufacturing technique and evaluates its performance quantitatively. We further develop and assess C-Sight, a novel VBTS sensor manufactured using this technique, focusing on its tactile reconstruction capabilities. Experimental results demonstrate that the monolithic manufacturing technique enhances VBTS production efficiency significantly. Also, the fabricated C-Sight sensor exhibits its reliable tactile perception and reconstruction capabilities, proofing the validity and feasibility of the monolithic manufacturing method.

[This corrects the article DOI: 10.1371/journal.pone.0044899.].

Abstract Background Panax quinquefolius saponin (PQS) is the main active component of Panax quinquefolius. Emerging evidence suggests that PQS exerts beneficial effects against cardiovascular diseases. However, the role and mechanism of PQS in vascular calcification are not unclear. The present study investigated the effects of PQS on the calcification of vascular smooth muscle cell (VSMCs). Methods The present study used calcification medium containing 3 mM inorganic phosphate (Pi) to induce rat VSMCs calcification. We investigated the effects of PQS on VSMCs calcification using alizarin red staining and alkaline phosphatase (ALP) activity assays. The intracellular reactive oxygen species (ROS) levels and the transcriptional activity of nuclear factor-erythroid 2-related factor 2 (Nrf2) were determined. The mRNA and protein expression levels of Nrf2, the antioxidant gene heme oxygenase-1 (HO-1), osteogenic markers, including runt-related transcription factor 2 (Runx2) and bone morphogenetic protein 2 (BMP2), and Kelch-like ECH-associated protein 1 (Keap1) were also measured. Results Treatment with Pi significantly increased intracellular calcium deposition and ALP activity, which were suppressed by PQS in a concentration-dependent manner. During VSMCs calcification, PQS inhibited the mRNA and protein expression of Runx2 and BMP2. PQS treatment reduced intracellular ROS production and significantly upregulated Nrf2 transcriptional activity and the expression of Nrf2 and its target antioxidant gene HO-1. PQS suppressed the Pi-induced protein expression of Keap1, which is an endogenous inhibitor of Nrf2. Keap1 siRNA treatment induced Nrf2 expression and downregulated Runx2 expression in the presence of Pi and PQS. Conclusion Taken together, these findings suggest that PQS could effectively inhibit VSMCs calcification by ameliorating oxidative stress and regulating osteogenic genes via the promotion of Nrf2 expression.

To improve the crystallinity and phase composition of the Hf0.5Zr0.5O2 films, the effects of annealing temperature on metal-ferroelectric-metal devices are studied by electrical measurements, nanobeam electron diffraction (NBD), and TEM. Forming gas annealing (FGA) at 400°C partially crystallizes the films, and more crystallization can be achieved by FGA at the increasing temperature of 500°C and 800°C. After FGA at 400°C and 500°C, metastable ferroelectric orthorhombic phase can be obtained as shown by electrical measurements and NBD. By the density functional theory (DFT) simulation, we propose that o-phase formation happens during both the annealing step as well as the cooling step. During the FGA at 800°C, the metastable orthorhombic phase overcomes the activation barrier and transforms into stable monoclinic phase. In this work, the systematic experimental study of phase transformation, free energy simulation for different phases, and schematic diagrams of free energy for phase transition at different temperatures are reported.

Ulcerative colitis (UC) is an incurable and highly complex digestive disease affecting millions of people worldwide. Compared to the current therapeutic drugs, bioactive peptides are more promising and safe substances as functional foods or drugs for the prevention and treatment of UC. The alcohol-soluble components from fermentation broth by fresh wheat germ and apple (AC-WGAF) were found to be effective in UC prevention in dextran sulfate sodium-induced mice in vivo. Herein, 4 novel peptides are identified from AC-WGAF by membrane ultrafiltration, recycling preparative high-performance liquid chromatography, and matrix-assisted laser desorption–ionization time-of-flight/time-of-flight mass spectrometry, possessing anti-colitis activity via using an in vitro model. One of those peptides named T24 (PVLGPVRGPFPLL) exhibited the most remarkable anti-colitis activity by preventing tight junction protein loss, maintaining epithelial barrier integrity, and promoting cell proliferation during in vitro and in vivo studies by regulating mitogen-activated protein kinase signaling pathways. Thus, T24 is a promising peptide as a functional food or novel drug for UC prevention and treatment.

High doses of silver compounds in water have been found to be extremely toxic to the cells of plants and animals. Removal then reutilization of silver ions as antibacterial materials in one step from wastewater is an urgent but challenging issue from the views of environment and economy. In this research, we reported a planar benzothiazole Schiff base derivative BTS1 that can selectively precipitate silver ions from wastewater over other metal ions through chemical precipitation. The precipitation reaction showed promising activity in the pH range of 6 to11, realizing a high silver ions precipitation ratio of 95.4%, and low LOD of 0.059 mg/L. More importantly, the filtrated precipitate BTS1-Ag can be directly used as an antibacterial material. The test papers coated with BTS1-Ag exhibited antibacterial activity against both gram-positive and gram-negative bacteria. This research provides a promising strategy for simultaneous removal and conversion of silver ions from wastewater into functional materials.

Cryogenic transition from metastable tetragonal phase (t-phase) to orthorhombic phase (o-phase) is crucial in achieving the desired ferroelectric characteristics. Observing the reversible transition from anti-ferroelectricity (AFE) to ferroelectricity (FE) in electrical characteristics, the cryogenic phase transition is experimentally analyzed in Hf0.5Zr0.5O2 alloys. Furthermore, the stabilized o-phase formation is more favorable when applying cryogenics to superlattice Hf0.5Zr0.5O2, manifesting a 23% increase in remanent polarization (Pr) at 77K. To theoretically clarify the emergence of phase transition with decreasing temperatures, Landau–Ginzburg–Devonshire theory and first-principle study are combined in this work. Based on the detailed calculation, the increasing relative free energy of the t-phase contributes to lowering the energy barrier when decreasing the temperature, making the convenient transitional pathway from metastable t- to o-phase. This work exhibits the cryogenic phase transition model involving t- and o-phases in Hf0.5Zr0.5O2 and presents a method to boost ferroelectricity for emerging HfO2-based cryo-device.

This paper is devoted to a nonautonomous SVIR epidemic model with relapse, that is, the recurrence rate is considered in the model. The permanent of the system is proved, and the result on the existence and uniqueness of globally attractive almost periodic solution of this system is obtained by constructing a suitable Lyapunov function. Some analysis for the necessity of considering the recurrence rate in the model is also presented. Moreover, some examples and numerical simulations are given to show the feasibility of our main results. Through numerical simulation, we have obtained the influence of vaccination rate and recurrence rate on the spread of the disease. The conclusion is that in order to control the epidemic of infectious diseases, we should increase the vaccination rate while reducing the recurrence rate of the disease.

Abstract Structured illumination microscopy (SIM) has become a widely used tool for insight into biomedical challenges due to its rapid, long-term, and super-resolution (SR) imaging. However, artifacts that often appear in SIM images have long brought into question its fidelity, and might cause misinterpretation of biological structures. We present HiFi-SIM, a high-fidelity SIM reconstruction algorithm, by engineering the effective point spread function (PSF) into an ideal form. HiFi-SIM can effectively reduce commonly seen artifacts without loss of fine structures and improve the axial sectioning for samples with strong background. In particular, HiFi-SIM is not sensitive to the commonly used PSF and reconstruction parameters; hence, it lowers the requirements for dedicated PSF calibration and complicated parameter adjustment, thus promoting SIM as a daily imaging tool.

Ulcerative colitis (UC) is a chronic systematic inflammation disorder with increasing incidence, unknown pathogenesis, limited drug treatment, and abundant medical expenses. Dietary intake, as a daily indispensable environment factor, is closely related to UC pathogenesis and prevention. The underlying interactions between dietary intake and UC progression are implicated with the modulation of gut microbiome as well as microbial metabolites, suggesting the complex and systematic characteristics of UC. However, the triangular relationships with dietary intake, gut microbiota homeostasis, and UC have not been well summarized so far. Here we review the recent studies of dietary intake on the regulation of gut microbiome homeostasis as well as modulation of UC progression. These findings suggest that varieties in dietary patterns result in the production of diverse microbial fermentation metabolites, which contribute to gut microbiome homeostasis through multiple manipulations including immune modulation, inflammation restriction as well as epithelial barrier maintenance, thus finally determine the fate of UC progression and give implications for functional food development for prevention and treatment of UC patients.

Utilizing the relativistically corrected Krammers-Henneberger (KH) potential in the high frequency Floquet theory (HFFT), the ground state of the positronium (Ps) atom in a linearly polarized high frequency super-intense laser field is investigated numerically. It is reported that the relativistic effect can prevent Ps from annihilating into γ rays, thus enhancing its stability. The underlying mechanism lies in the reconstruction of the electron wavefunction into a figure-of-eight shape, which leads to an effective reduction of the electron-positron overlapping probability.

HCV cell-culture system uses hepatoma-derived cell lines for efficient virus propagation. Tumor cells cultured in glucose undergo active aerobic glycolysis, but switch to oxidative phosphorylation for energy production when cultured in galactose. Here, we investigated whether modulation of glycolysis in hepatocytes affects HCV infection. We showed HCV release, but not entry, genome replication or virion assembly, is significantly blocked when cells are cultured in galactose, leading to accumulation of intracellular infectious virions within multivesicular body (MVB). Blockade of the MVB-lysosome fusion or treatment with pro-inflammatory cytokines promotes HCV release in galactose. Furthermore, we found this glycometabolic regulation of HCV release is mediated by MAPK-p38 phosphorylation. Finally, we showed HCV cell-to-cell transmission is not affected by glycometabolism, suggesting that HCV cell-to-supernatant release and cell-to-cell transmission are two mechanistically distinct pathways. In summary, we demonstrated glycometabolism regulates the efficiency and route of HCV release. We proposed HCV may exploit the metabolic state in hepatocytes to favor its spread through the cell-to-cell transmission in vivo to evade immune response.

To meet the need for micro-volume devices for high-precision measurement of temperature, Cu-Constantan (CuNi45) thin films with a novel array structure of thermo-electrodes were designed and fabricated. The thermo-electrodes on the probe-type substrate were deposited by magnetron sputtering technology and the profiling mask was prepared by 3D printing technology. The comprehensive performance of the temperature sensor was improved by systematic optimization of the heat treatment process and accuracy correction algorithm. Results showed that the sensor can measure with an accuracy of up to ±0.19%FS from −60 °C to 200 °C. The three-dimensional probe array temperature sensor shows great advantages in sensitivity, reliability resolution, stability, and measurement accuracy.

Sorghum [Sorghum bicolor (L.) Moench] seed germination is sensitive to salinity, and seed priming is an effective method for alleviating the negative effects of salt stress on seed germination. However, few studies have compared the effects of different priming agents on sorghum germination under salt stress. In this study, we quantified the effects of priming with distilled water (HP), sodium chloride (NaCl), potassium chloride (KCl), calcium chloride (CaCl2), and polyethylene glycol (PEG) on sorghum seed germination under 150 mM NaCl stress. The germination potential, germination rate, germination index, vigor index, root length, shoot length, root fresh weight, shoot fresh weight, root dry weight, and shoot dry weight were significantly reduced by salt stress. Different priming treatments alleviated the germination inhibition caused by salt stress to varying degrees, and 50 mM CaCl2 was the most effective treatment. In addition, the mitigation effect of priming was stronger on root traits than on shoot traits. Mitigation efficacy was closely related to both the type of agent and the concentration of the solution. Principal component analysis showed that all concentrations of CaCl2 had higher scores and were clearly distinguished from other treatments based on their positive effects on all germination traits. The effects of the other agents varied with concentration. The priming treatments were divided into three categories based on their priming efficacy, and the 50, 100, and 150 mM CaCl2 treatments were placed in the first category. The 150 mM KCl, 10% PEG, HP, 150 mM NaCl, 30% PEG, and 50 mM KCl treatments were placed in the second category, and the 100 mM NaCl, 100 mM KCl, 20% PEG, and 50 mM NaCl treatments were least effective and were placed in the third category. Choosing appropriate priming agents and methods for future research and applications can ensure that crop seeds germinate healthily under saline conditions.

A reaction-diffusion (R-D) heroin epidemic model with relapse and permanent immunization is formulated. We use the basic reproduction number R0 to determine the global dynamics of the models. For both the ordinary differential equation (ODE) model and the R-D model, it is shown that the drug-free equilibrium is globally asymptotically stable if R0≤1, and if R0>1, the drug-addiction equilibrium is globally asymptotically stable. Some numerical simulations are also carried out to illustrate our analytical results.

Abstract The Poisson type inequalities, which were improved by Shu, Chen, and Vargas-De-Teón (J. Inequal. Appl. 2017:114, 2017), are generalized by using Poisson identities involving modified Poisson kernel functions with respect to a cone. New generalizations of improved Poisson–Sch type inequalities are obtained by using the generalized Montgomery identity associated with the Schrödinger operator. As applications in quantum calculus, we estimate the size of weighted Schrödingerean harmonic Bergman functions in the upper half space.

Abstract The aim of this paper is to investigate the existence of weak solutions for a two-dimensional Schrödinger equation with a singular potential in C+ $\mathbb{C}_{+}$. Under appropriate assumptions on the nonlinearity, we introduce a new type of quantum calculus via the Morse theory and variational methods. By applying Schrödinger type inequalities and the well-known Banach fixed point theorem in conjunction with the technique of measures of weak noncompactness, the new and more accurate estimations for boundary behaviors of them are also deduced.

The synthesis of anthocyanin pigments in plants is known to be regulated by multiple mechanisms, including epigenetic regulation; however, the contribution of the RNA-directed DNA methylation (RdDM) pathway is not well understood. Here, we used bisulfite sequencing and Real Time (RT)-quantitative (q) PCR to analyze the methylation level of the promoter of constitutively photomorphogenic 1 (McCOP1) from Malus cv. spp, a gene involved in regulating anthocyanin biosynthesis. The CHH methylation level of the McCOP1 promoter was negatively correlated with McCOP1 RNA expression, and inhibiting DNA methylation caused decreased methylation of the McCOP1 promoter and asymmetric cytosine CHH methylation. We observed that the McCOP1 promoter was a direct target of the RdDM pathway argonaute RISC component 4 (McAGO4) protein, which bound to a McCOP1 promoter GGTTCGG site. Bimolecular fluorescence complementation (BIFC) analysis showed that RNA-directed DNA methylation (McRDM1) interacted with McAGO4 and another RdDM protein, domains rearranged methyltransferase 2 (McDRM2), to regulate the CHH methylation of the McCOP1 promoter. Detection of CHH methylation and COP1 gene expression in the Arabidopsis thalianaatago4, atdrm2 and atrdm1 mutants showed that RDM1 is the effector of the RdDM pathway. This was confirmed by silencing McRDM1 in crabapple leaves or apple fruit, which resulted in a decrease in McCOP1 CHH methylation and an increase in McCOP1 transcript levels, as well as in anthocyanin accumulation. In conclusion, these results show that the RdDM pathway is involved in regulating anthocyanin accumulation through CHH methylation of the McCOP1 promoter.

Waterlogging stress is one of the most important abiotic stresses limiting sorghum growth and development. Consequently, the responses of sorghum to waterlogging must be monitored and studied. This study investigated changes in the leaf water status, xylem exudation rate, leaf anatomical structure, leaf temperature and photosynthetic performance. Waterlogging-tolerant (Jinuoliang 01, abbreviated JN01) and waterlogging-sensitive (Jinza 31, abbreviated JZ31) sorghum cultivars were planted in pots. The experiment was carried out using a split block design with three replications. Waterlogging stress was imposed at the sorghum five-leaf stage. The leaf free water content (FWC) and relative water content (RWC) decreased under the waterlogged condition. The leaf thickness was thinner under the waterlogged condition, and the main changes occurred in the upper epidermal and mesophyll cells. Gas exchange parameters and the xylem exudation rate were also restrained by waterlogging; however, greater responses of these parameters were observed in JZ31. JZ31 had a higher leaf-air temperature difference (ΔT) than JN01. We found that changes in ΔT were always consistent with changes in the RWC and the gas exchange parameters. ΔT was significantly associated with the leaf RWC, photosynthetic rate (Pn) and transpiration rate (Tr). The results suggest that ΔT may be an indicator reflecting the water status in leaves and can be used to evaluate the tolerance of sorghum to waterlogging.

Virus infection has drawn extensive attention since it causes serious or even deadly diseases, consequently inducing a series of social and public health problems. Caveolin-1 is the most important structural protein of caveolae, a membrane invagination widely known for its role in endocytosis and subsequent cytoplasmic transportation. Caveolae/caveolin-1 is tightly associated with a wide range of biological processes, including cholesterol homeostasis, cell mechano-sensing, tumorigenesis, and signal transduction. Intriguingly, the versatile roles of caveolae/caveolin-1 in virus infections have increasingly been appreciated. Over the past few decades, more and more viruses have been identified to invade host cells via caveolae-mediated endocytosis, although other known pathways have been explored. The subsequent post-entry events, including trafficking, replication, assembly, and egress of a large number of viruses, are caveolae/caveolin-1-dependent. Deprivation of caveolae/caveolin-1 by drug application or gene editing leads to abnormalities in viral uptake, viral protein expression, or virion release, whereas the underlying mechanisms remain elusive and must be explored holistically to provide potential novel antiviral targets and strategies. This review recapitulates our current knowledge on how caveolae/caveolin-1 functions in every step of the viral infection cycle and various relevant signaling pathways, hoping to provide a new perspective for future viral cell biology research.

A new scheme is proposed to improve the quality of proton beams via ultra-intense laser pulse interacting with double plasma targets, which consist of a pre-target with relatively low density and a main target with high density. Both one- and two-dimensional Particle-in-Cell simulations show that, the using of an appropriate pre-target can help to obtain a much stronger longitudinal charge separation field in contrast to using only the main target. And proton beam with lower momentum divergence, better monochromaticity and collimation, as well as higher current density is generated. Moreover, due to the strengthened coupling between the laser pulse and targets, the energy conversion from laser pulse to protons is also increased.

This paper proposes a new quantum control method which controls the Shannon entropy of quantum systems. For both discrete and continuous entropies, controller design methods are proposed based on probability density function control, which can drive the quantum state to any target state. To drive the entropy to any target at any prespecified time, another discretization method is proposed for the discrete entropy case, and the conditions under which the entropy can be increased or decreased are discussed. Simulations are done on both two- and three-dimensional quantum systems, where division and prediction are used to achieve more accurate tracking.

Nuclear factor E2-related factor 2 (Nrf2) appears to be an attractive therapeutic target for the treatment of cardiac disease. We investigated whether a synthetic triterpenoid derivative of dihydro-CDDO-trifluoroethylamide (dh404), a novel Nrf2 activator, protects against pathological cardiac responses to hemodynamic stress in mice.Cardiac maladaptive remodeling and dysfunction were established by transverse aortic constriction (TAC) in mice. Hypertrophic growth of rat neonatal cardiomyocytes was induced by angiotensin II (Ang II). Cell death of rat neonatal cardiomyocytes was induced with hydrogen peroxide (H₂O₂). Cellular proliferation of rat neonatal cardiac fibroblasts was induced by Ang II, norepinephrine (NE) and phenylephrine (PE). Protein expression was assessed by immunochemical staining and Western blots. Gene expression was determined by real time reverse transcription-polymerase chain reaction (Q-PCR).TAC suppressed myocardial Nrf2 expression, increased myocardial 4-hydroxy-2-nonenal and 8-hydroxydeoxyguanosine levels, and induced cardiac hypertrophy, fibrosis and apoptosis, and overt heart failure and death in mice. Administration of dh404 inhibited the pathological cardiac remodeling and dysfunction, and reduced the mortality. Moreover, dhd404 elevated myocardial levels of Nrf2 and Nrf2 nuclear translocation with a dramatic suppression of the oxidative stress in the heart. Dh404 inhibited hypertrophic growth and death in primary culture of rat neonatal cardiomyocytes and suppressed proliferation in primary culture of rat neonatal cardiac fibroblasts. However, these effects of dh404 were blunted by knocking down of Nrf2.These findings demonstrate that dh404 prevents pathological cardiac remodeling and dysfunction by activating Nrf2, indicating a therapeutic potential of dh404 for cardiac disease.