135 results on '"Yichen Tian"'
Search Results
2. Research on Forage–Livestock Balance in the Three-River-Source Region Based on Improved CASA Model
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Chenlu Hu, Yichen Tian, Kai Yin, Huiping Huang, Liping Li, and Qiang Chen
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Three–River–Source Region ,forage–livestock balance ,CASA model ,alpine grassland ,livestock carrying capacity ,Science - Abstract
As an important ecological barrier and a crucial base for animal husbandry in China, the forage–livestock balance in the Three-River-Source Region (TRSR) directly impacts both the degradation and recovery of grassland. This study examines the forage–livestock balance in the TRSR over the past 13 years (2010–2022) by calculating both the theoretical and actual livestock carrying capacity, thereby providing a scientific basis for regional animal husbandry policies. Firstly, the Carnegie–Ames–Stanford Approach (CASA) model was improved to fit the specific characteristics of alpine grassland ecosystem in the TRSR. This enhanced model was subsequently used to calculate the net primary productivity (NPP) of the grassland, from which the regional grassland yield and theoretical livestock carrying capacity were derived. Secondly, the actual livestock carrying capacity was calculated and spatialized based on the number of regional year-end livestock. Finally, the livestock carrying pressure index was determined using both the theoretical and actual livestock carrying capacity. The results revealed several key findings: (1) The average grassland NPP in the TRSR was 145.44 gC/m2, the average grassland yield was 922.7 kg/hm2, and the average theoretical livestock carrying capacity was 0.55 SU/hm2 from 2010 to 2022. Notably, all three metrics showed an increasing trend over the past 13 years, which indicates the rise in grassland vegetation activities. (2) The average actual livestock carrying capacity over the 13-year period was 0.46 SU/hm2, showing a decreasing trend on the whole. The spatial distribution displayed a pattern of higher capacity in the east and lower in the west. (3) Throughout the 13 years, the TRSR generally maintained a forage–livestock balance, with an average livestock carrying pressure index of 0.96 (insufficient). However, the trend of livestock carrying pressure is on the rise, with serious overloading observed in the western part of Qumalai County and the northern part of Tongde County. Slight overloading was also noted in Zhiduo, Maduo, and Zeku Counties. Notably, Tanggulashan Town, Zhiduo, Qumalai, and Maduo Counties showed significant increases in livestock carrying pressure, while Zaduo County and the eastern regions experienced significant decreases. In conclusion, this study not only provides feasible technical methods for assessing and managing the forage–livestock balance in the TRSR but also contributes significantly to the sustainable development of the region’s grassland ecosystem and animal husbandry industry.
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- 2024
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3. Recombinant-attenuated Salmonella enterica serovar Choleraesuis vector expressing the PlpE protein of Pasteurella multocida protects mice from lethal challenge
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Guodong Zhou, Jiashuo Tian, Yichen Tian, Qifeng Ma, Quan Li, Shifeng Wang, and Huoying Shi
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Recombinant attenuated Salmonella vaccine ,P. multocida ,PlpE ,Vaccine candidate ,Immune responses ,Veterinary medicine ,SF600-1100 - Abstract
Abstract Background Bacterial surface proteins play key roles in pathogenicity and often contribute to microbial adhesion and invasion. Pasteurella lipoprotein E (PlpE), a Pasteurella multocida (P. multocida) surface protein, has recently been identified as a potential vaccine candidate. Live attenuated Salmonella strains have a number of potential advantages as vaccine vectors, including immunization with live vector can mimic natural infections by organisms, lead to the induction of mucosal, humoral, and cellular immune responses. In this study, a previously constructed recombinant attenuated Salmonella Choleraesuis (S. Choleraesuis) vector rSC0016 was used to synthesize and secrete the surface protein PlpE of P. multocida to form the vaccine candidate rSC0016(pS-PlpE). Subsequently, the immunogenicity of S. Choleraesuis rSC0016(pS-PlpE) as an oral vaccine to induce protective immunity against P. multocida in mice was evaluated. Results After immunization, the recombinant attenuated S. Choleraesuis vector can efficiently delivered P. multocida PlpE protein in vivo and induced a specific immune response against this heterologous antigen in mice. In addition, compared with the inactivated vaccine, empty vector (rSC0016(pYA3493)) and PBS immunized groups, the rSC0016(pS-PlpE) vaccine candidate group induced higher antigen-specific mucosal, humoral and mixed Th1/Th2 cellular immune responses. After intraperitoneal challenge, the rSC0016(pS-PlpE) immunized group had a markedly enhanced survival rate (80%), a better protection efficiency than 60% of the inactivated vaccine group, and significantly reduced tissue damage. Conclusions In conclusion, our study found that the rSC0016(pS-PlpE) vaccine candidate provided good protection against challenge with wild-type P. multocida serotype A in a mouse infection model, and may potentially be considered for use as a universal vaccine against multiple serotypes of P. multocida in livestock, including pigs.
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- 2023
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4. Targeted macrophage phagocytosis by Irg1/itaconate axis improves the prognosis of intracerebral hemorrhagic stroke and peritonitisResearch in context
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Zhaoli Luo, Ziyang Sheng, Liye Hu, Lei Shi, Yichen Tian, Xiaochu Zhao, Wei Yang, Zhongnan Xiao, Danmin Shen, Weihua Wu, Ting Lan, Boqian Zhao, Xiaogang Wang, Nan Zhuang, Jian-Nan Zhang, Yamei Wang, Yabin Lu, Liyong Wang, Chenguang Zhang, Peipei Wang, Jing An, Fei Yang, and Qian Li
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Irg1 ,Itaconate ,Macrophage ,Phagocytosis ,Intracerebral hemorrhagic stroke ,Peritonitis ,Medicine ,Medicine (General) ,R5-920 - Abstract
Summary: Background: Macrophages are innate immune cells whose phagocytosis function is critical to the prognosis of stroke and peritonitis. cis-aconitic decarboxylase immune-responsive gene 1 (Irg1) and its metabolic product itaconate inhibit bacterial infection, intracellular viral replication, and inflammation in macrophages. Here we explore whether itaconate regulates phagocytosis. Methods: Phagocytosis of macrophages was investigated by time-lapse video recording, flow cytometry, and immunofluorescence staining in macrophage/microglia cultures isolated from mouse tissue. Unbiased RNA-sequencing and ChIP-sequencing assays were used to explore the underlying mechanisms. The effects of Irg1/itaconate axis on the prognosis of intracerebral hemorrhagic stroke (ICH) and peritonitis was observed in transgenic (Irg1flox/flox; Cx3cr1creERT/+, cKO) mice or control mice in vivo. Findings: In a mouse model of ICH, depletion of Irg1 in macrophage/microglia decreased its phagocytosis of erythrocytes, thereby exacerbating outcomes (n = 10 animals/group, p
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- 2024
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5. Efficacy comparison of immune combination therapies in subgroups for advanced hepatocellular carcinoma patients: Systematic review and network meta-analysis.
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Yani Wang, Wanyee Lau, Yafei Li, Yichen Tian, Yongrong Lei, Feng Xia, and Jianhua Wang
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Medicine ,Science - Abstract
BackgroundThere is a lack of precision in the immunotherapy strategy tailored for patients exhibiting diverse clinical characteristics. This study aims to employ a rigorous network meta-analysis (NMA) approach to systematically evaluate the effectiveness of immune-combination therapies among patients with advanced hepatocellular carcinoma, taking into account their varying clinico-characteristics.MethodsStudies were retrieved from PubMed, Embase, Cochrane Library, and Web of Science databases. The included first-line phase III studies were categorized into three types: immunotherapy combined with anti-angiogenetic agents, immunotherapy combined with tyrosine kinase inhibitors, and dual immunotherapy, with sorafenib serving as the control group. The primary endpoint used to assess efficacy was overall survival (OS), facilitating a comparative analysis among the three treatment modalities. Furthermore, subgroup analyses were conducted to evaluate the varying effectiveness for patients with diverse clinico-characteristics. Secondary outcome measures included progression-free survival, objective response rate, and toxicity assessment.ResultsA total of 6 studies were included in the NMA, encompassing a cohort of 3840 patients. The results revealed that immunotherapy combined with anti-angiogenetic agents exhibited a significantly enhanced therapeutic effect in terms of improving OS compared to sorafenib (HR = 0.61, 95% CrI, 0.42-0.90). Furthermore, based on various clinicopathological features, this combination therapy demonstrated superior OS responses in specific patient subgroups: BCLC C (HR = 0.63, 95% CrI, 0.42-0.93), ECOG 1 (HR = 0.57, 95% CrI, 0.36-0.91), with extrahepatic spread (EHS) (HR = 0.59, 95% CrI, 0.37-0.92), alpha fetoprotein (AFP)ConclusionsImmunotherapy combined with anti-angiogenetic agents could represent the most effective first-line intervention for achieving improved OS, particularly in patients with viral hepatitis positivity.
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- 2024
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6. Comparison of conventional and unconventional obesity indices associated with new-onset hypertension in different sex and age populations
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Xueyao Zhang, Guangxiao Li, Chuning Shi, Yichen Tian, Linlin Zhang, Hongyu Zhang, and Yingxian Sun
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Medicine ,Science - Abstract
Abstract We aimed to compare the relationship between hypertension and obesity-related anthropometric indices (waist circumference [WC], waist-height ratio, waist-hip ratio [WHR], and body mass index; unconventional: new body shape index [ABSI] and body roundness index [BRI]) to identify best predictors of new-onset hypertension. The study included 4123 adult participants (2377 women). Hazard ratios (HRs) and 95% confidence intervals (CIs) were determined using a Cox regression model to estimate the risk of new-onset hypertension with respect to each obesity index. In addition, we assessed the predictive value of each obesity index for new-onset hypertension using area under the receiver operating characteristic curve (AUC) after adjusting for common risk factors. During the median follow-up of 2.59 years, 818 (19.8%) new hypertension cases were diagnosed. The non-traditional obesity indices BRI and ABSI had predictive value for new-onset hypertension; however, they were not better than the traditional indexes. WHR was the best predictor of new-onset hypertension in women aged ≤ 60 and > 60 years, with HRs of 2.38 and 2.51 and AUCs of 0.793 and 0.716. However, WHR (HR 2.28, AUC = 0.759) and WC (HR 3.24, AUC = 0.788) were the best indexes for predicting new-onset hypertension in men aged ≤ 60 and > 60 years, respectively.
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- 2023
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7. Identification of Ecological Sources Using Ecosystem Service Value and Vegetation Productivity Indicators: A Case Study of the Three-River Headwaters Region, Qinghai–Tibetan Plateau, China
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Xinyi Feng, Huiping Huang, Yingqi Wang, Yichen Tian, and Liping Li
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ecological sources ,ecosystem services value ,vegetation productivity ,Three-River Headwaters Region ,Science - Abstract
As a crucial component of the ecological security pattern, ecological source (ES) plays a vital role in providing ecosystem service value (ESV) and conserving biodiversity. Previous studies have mostly considered ES only from either landscape change pattern or ecological function perspectives, and have ignored their integration and spatio-temporal evolutionary modeling. In this study, we proposed a multi-perspective framework for the spatio-temporal characteristics of ES by ESV incorporating landscape aesthetics, carbon sink characteristics, ecological quality, and kernel NDVI (kNDVI). By integrating the revised ESV and the kernel normalized difference vegetation index as a foundation, we employed the spatial priority model to identify ES. This improvement aims to yield a more practical and specific ESV result. Applying this framework to the Three-River Headwaters Region (TRHR), a significant spatio-temporal change in ecological sources has been observed from 2000 to 2020. This performance provided a reference for ecological conservation in the TRHR. The results indicate that this ecological source identification framework has reliable accuracy and efficiency compared with the existing NRs in the TRHR. This method could reveal more precise spatio-temporal distributions of ES, enhancing ecosystem integrity and providing technical modeling support for developing cross-scale spatial planning and management strategies for nature reserve boundaries. The framework proposed in our research could serve as a reference for building ecological networks in other ecologically fragile areas.
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- 2024
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8. Genomic profiling, prognosis, and potential interventional targets in young and old patients with cholangiocarcinoma
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Junhua Wang, Yaoting Shi, Jianbo Chen, Juying Liu, Xiaotian Zhao, Jiaohui Pang, Ximin Sun, Yichen Tian, Qiuxiang Ou, Feng Xia, and Yunjie Chen
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genomic profiling ,prognosis ,patient age ,cholangiocarcinoma ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Molecular mechanisms behind potentially inferior prognosis of old cholangiocarcinoma (CCA) patients are unclear. Prevalence of interventional targets and the difference between young and old CCA patients are valuable for promising precision medicine. A total of 188 CCA patients with baseline tumor tissue samples were subgrouped into the young (≤45 years) and old (>45 years) sub-cohorts. Somatic and germline mutation profiles, differentially enriched genetic alterations, and actionable genetic alterations were compared. An external dataset was used for the validation of molecular features and the comparison of overall survival (OS). Compared to young patients, KRAS alterations were more common in old patients (P = .04), while FGFR2 fusions were less frequent (P = .05). TERT promoter mutations were exclusively detected in old patients. The external dataset (N = 392) revealed no significant difference in OS between young and old patients; however, old patient-enriched KRAS (hazard ratio [HR]: 1.96, 95% confidence interval [CI]: 1.37–2.80) and TERT alterations (HR: 2.03, 95% CI: 1.22–3.38) were associated with inferior OS. Approximately 38.3% of patients were identified of actionable oncogenic mutations indicative of a potential response to targeted therapy or immunotherapy. Actionable FGFR2 fusions (P = .01) and BRAFV600E (P = .04) mutations were more frequent in young females than old patients. The enrichment of KRAS/TERT alterations in CCA patients over 45 years resulted in inferior OS. Approximately one-third of CCA patients were eligible for targeted therapy or immunotherapy given the actionable mutations carried, especially young females.
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- 2023
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9. Investigating the crosstalk between chronic stress and immune cells: implications for enhanced cancer therapy
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YongRong Lei, Fenghui Liao, YiChen Tian, YaNi Wang, Feng Xia, and JianHua Wang
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chronic stress ,tumor microenvironment ,immunotherapy ,anticancer treatments ,sympathetic nervous system ,neural-immune interactions ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
Chronic stress has a substantial influence on the tumor microenvironment (TME), leading to compromised effectiveness of anti-cancer therapies through diverse mechanisms. It disrupts vital functions of immune cells that play a critical role in anti-tumor immunity, such as the inhibition of dendritic cells (DCs) and lymphocytes, while simultaneously enhancing the activity of immune cells that support tumor growth, such as myeloid-derived suppressor cells and tumor-associated macrophages. Furthermore, chronic stress exerts a significant impact on crucial mechanisms within the TME, including angiogenesis, DNA repair, hypoxia, extracellular matrix deposition, and tumor metabolism. These alterations in the TME, induced by stress, result from the activation of the hypothalamic–pituitary–adrenal axis and sympathetic nervous system, in conjunction with epigenetic modifications. In conclusion, chronic stress significantly influences the TME and impedes the efficacy of anti-cancer treatments, underscoring the importance of targeting stress pathways to improve therapeutic results.
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- 2023
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10. Effect of various hepatectomy procedures on circulating tumor cells in postoperative patients: a case-matched comparative study
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YongRong Lei, XiShu Wang, YiChen Tian, Rong Xu, Jun Pei, YuNa Fu, Heng Sun, YaNi Wang, Ping Zheng, Feng Xia, and JianHua Wang
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circulating tumor cells ,laparoscopic liver resection ,open hepatectomy ,recurrence ,EMT ,liquid biopsy ,Medicine (General) ,R5-920 - Abstract
BackgroundThe objective of this study is to elucidate the prevalence of systemic circulating tumor cells (CTCs) prior to and following resection of hepatocellular carcinoma (HCC), and to compare the disparities in postoperative CTCs in terms of quantity and classifications between the open liver resection (OPEN) and laparoscopic liver resection (LAP) cohorts.Patients, materials, and methodsFrom September 2015 to May 2022, 32 consecutive HCC patients who underwent laparoscopic liver resection at Southwest Hospital were retrospectively enrolled in this study. The clinicopathological data were retrieved from a prospectively collected computer database. Patients in the OPEN group matched at a 1:1 ratio with patients who underwent open liver resection during the study period on age, gender, tumor size, number of tumors, tumor location, hepatitis B surface antigen (HBsAg) positivity, alpha-fetoprotein (AFP) level, TNM and Child-Pugh staging from the database of patients to form the control group. The Can-Patrol CTC enrichment technique was used to enrich and classify CTCS based on epithelial-mesenchymal transformation phenotypes. The endpoint was disease-free survival (DFS), and the Kaplan–Meier method and multiple Cox proportional risk model were used to analyze the influence of clinicopathological factors such as total CTCs and CTC phenotype on prognosis.ResultsThe mean age of the 64 patients with primary liver cancer was 52.92 years (23–71), and 89.1% were male. The postoperative CTC clearance rate was more significant in the OPEN group. The total residual CTC and phenotypic CTC of the LAP group were significantly higher than those of the OPEN group (p = 0.017, 0.012, 0.049, and 0.030, respectively), which may increase the possibility of metastasis (p = 0.042). In Kaplan–Meier analysis, DFS was associated with several clinicopathological risk factors, including Barcelona Clinical Liver Cancer (BCLC) stage, tumor size, and vascular invasion. Of these analyses, BCLC Stage [p = 0.043, HR (95% CI) =2.03(1.022–4.034)], AFP [p = 0.007, HR (95% CI) =1.947 (1.238–3.062)], the number of positive CTCs [p = 0.004, HR (95% CI) =9.607 (2.085–44.269)] and vascular invasion [p = 0.046, HR (95% CI) =0.475 (0.22–1.023)] were significantly associated with DFS.ConclusionIn comparison to conventional OPEN technology, LAP technology has the capacity to augment the quantity of epithelial, mixed, and mesenchymal circulating tumor cells (CTCs). Following the surgical procedure, there was a notable increase in the total CTCs, epithelial CTCs, and mixed CTCs within the LAP group, indicating a potential drawback of LAP in facilitating the release of CTCs.
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- 2023
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11. Vegetation and Evapotranspiration Responses to Increased Atmospheric Vapor Pressure Deficit across the Global Forest
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Rihong Wen, Meiou Qin, Peng Jiang, Feiyun Yang, Bin Liu, Mengyuan Zhu, Yuan Fang, Yichen Tian, and Bo Shang
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vapor pressure deficit ,LAI ,evapotranspiration ,water availability ,global forests ,Meteorology. Climatology ,QC851-999 - Abstract
A forest is vulnerable to drought and plays important roles in the regulation of carbon and water cycling in a terrestrial ecosystem. Atmospheric vapor pressure deficit (VPD) has been identified as an increasingly major factor in plant functioning and has been established as a main contributor to recent drought-induced plant mortality, independent of other drivers associated with climate change. However, most previous studies have focused on the effects of climate warming and CO2 enrichment on vegetation growth, without considering the effects of an increased VPD on vegetation growth and evapotranspiration (ET) in forest ecosystems. This could lead to a large uncertainty in estimating the variability in forest carbon sinks. Based on the long-term satellite data, we investigated the response of the leaf area index (LAI) and ET to the VPD via a partial correlation analysis in this study. We also examined the temporal variability in the partial coefficients within a ten-year moving window. The results showed that over 50% of the region displayed a negative partial correlation between the LAI, ET, and VPD, and those pixels were mainly concentrated in North America and the plains of Eastern Europe. Regions with a negative trend of partial correlation in both the LAI and ET are mostly located in the plains of Eastern Europe and the Siberian Plain of western Russia, while the positive trend is mainly in South America. The plains of Eastern Europe are becoming drier, which was proved by the interannual trend of the Standardized Precipitation Evapotranspiration Index (SPEI) and soil water content (SWC). Additionally, the LAI and ET in those areas exhibited a significant positive correlation with the SWC based on the moving window average. This study suggests that the role of the VPD on vegetation will become increasingly prominent in the context of future climate change for the forest ecosystem.
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- 2024
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12. A New High-Resolution Rural Built-Up Land Extraction Method Based on Artificial Surface Index with Short-Wave Infrared Downscaling
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Wenlu Zhu, Chao Yuan, Yichen Tian, Yingqi Wang, Liping Li, and Chenlu Hu
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rural built-up land extraction ,Artificial Surface Index ,SWIR downscaling ,high-resolution remote sensing image ,Science - Abstract
The complexity of surface characteristics in rural areas poses challenges for accurate extraction of built-up areas from remote sensing images. The Artificial Surface Index (ASI) emerged as a novel and accurate built-up land index. However, the absence of short-wave infrared (SWIR) bands in most high-resolution (HR) images restricts the application of index-based methods in rural built-up land extraction. This paper presents a rapid extraction method for high-resolution built-up land in rural areas based on ASI. Through the downscaling techniques of random forest (RF) regression, high-resolution SWIR bands were generated. They were then combined with visible and near-infrared (VNIR) bands to compute ASI on GaoFen-2 (GF-2) images (called ASIGF). Furthermore, a red roof index (RRI) was designed to reduce the probability of misclassifying built-up land with bare soil. The results demonstrated that SWIR downscaling effectively compensates for multispectral information absence in HR imagery and expands the applicability of index-based methods to HR remote sensing data. Compared with five other indices (UI, BFLEI, NDBI, BCI, and PISI), the combination of ASI and RRI achieved the optimal performance in built-up land enhancement and bare land suppression, particularly showcasing superior performance in rural built-up land extraction.
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- 2024
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13. Outer membrane protein A (OmpA) may be used as a novel target to enrich and detect Escherichia coli in milk samples
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Yichen Tian, Kaiqing Yang, Yaoqiang Shi, Jinyang Zhang, Qinqin Han, Xueshan Xia, and Yuzhu Song
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Escherichia coli ,OmpA ,milk ,immunomagnetic bead ,sensitivity ,Dairy processing. Dairy products ,SF250.5-275 ,Dairying ,SF221-250 - Abstract
ABSTRACT: In recent years, food safety incidents caused by Escherichia coli have occurred and have endangered human health. Due to the complex matrix of milk samples and the long pretreatment time, the existing methods cannot quickly detect E. coli in milk samples. It is necessary to enrich the E. coli in the complex matrix to improve the detection sensitivity. The E. coli outer membrane protein A (OmpA) is widely present on the cell membrane of E. coli and may be used as a new target to enrich E. coli. In this study, the purified recombinant OmpA protein was used to immunize BALB/c mice to produce polyclonal antibody. Immunomagnetic beads were combined with the polyclonal antibody to enrich the E. coli in the artificially contaminated milk samples. The products of immunoprecipitation were further used for PCR assay. The bacteria in the PCR sample can be pre-enriched, and the limit of detection is 10 × 100 cfu/mL, which is about 100 times more sensitive than samples not processed by this method. Then, the artificially contaminated milk, coffee, juice, and soybean milk samples were tested separately, and it was found that the E. coli gene could be amplified. The whole analysis time was about 120 min, including the enrichment of bacteria and the detection of eluate. We found that OmpA combined with immunomagnetic beads was more efficient, fast, and convenient than the conventional method. Bacteria can be enriched more efficiently without extracting genomic DNA and culturing bacteria. Therefore, this method has potential value for improving the detection sensitivity and shortening the detection time of E. coli in food samples.
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- 2022
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14. The role of TolA, TolB, and TolR in cell morphology, OMVs production, and virulence of Salmonella Choleraesuis
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Quan Li, Zheng Li, Xia Fei, Yichen Tian, Guodong Zhou, Yuhan Hu, Shifeng Wang, and Huoying Shi
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Tol–Pal system ,tolA ,tolB ,tolR ,Salmonella Choleraesuis ,Outer membrane vesicles ,Biotechnology ,TP248.13-248.65 ,Microbiology ,QR1-502 - Abstract
Abstract The Tol–Pal system of Gram-negative bacteria is necessary for maintaining outer membrane integrity. It is a multiprotein complex of five envelope proteins, TolQ, TolR, TolA, TolB, and Pal. These proteins were first investigated in E. coli, and subsequently been identified in many other bacterial genera. However, the function of the Tol–Pal system in Salmonella Choleraesuis pathogenesis is still unclear. Here, we reported the role of three of these proteins in the phenotype and biology of S. Choleraesuis. We found that mutations in tolA, tolB, and tolR caused severe damage to the cell wall, which was supported by observing the microstructure of spherical forms, long chains, flagella defects, and membrane blebbing. We confirmed that all the mutants significantly decreased S. Choleraesuis survival when exposed to sodium deoxycholate and exhibited a high sensitivity to vancomycin, which may be explained by the disruption of envelope integrity. In addition, tolA, tolB, and tolR mutants displayed attenuated virulence in a mouse infection model. This could be interpreted as a series of defective phenotypes in the mutants, such as severe defects in envelope integrity, growth, and motility. Further investigation showed that all the genes participate in outer membrane vesicles (OMVs) biogenesis. Interestingly, immunization with OMVs from ΔtolB efficiently enhanced murine viability in contrast to OMVs from the wild-type S. Choleraesuis, suggesting its potential use in vaccination strategies. Collectively, this study provides an insight into the biological role of the S. Choleraesuis Tol–Pal system.
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- 2022
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15. Oral Immunization with Attenuated Salmonella Choleraesuis Expressing the P42 and P97 Antigens Protects Mice against Mycoplasma hyopneumoniae Challenge
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Guodong Zhou, Yichen Tian, Jiashuo Tian, Qifeng Ma, Shan Huang, Quan Li, Shifeng Wang, and Huoying Shi
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Salmonella Choleraesuis ,Mycoplasma hyopneumoniae ,P42 ,P97 ,recombinant vaccine ,Microbiology ,QR1-502 - Abstract
ABSTRACT Mycoplasma hyopneumoniae (M. hyopneumoniae, Mhp) is the etiological agent of swine enzootic pneumonia (EP), which has been associated with considerable economic losses due to reduced daily weight gain and feed efficiency. Adhesion to the cilia is important for Mhp to colonize the respiratory epithelium. Therefore, a successful vaccine must induce broad Mhp-specific immune responses at the mucosal surface. Recombinant attenuated Salmonella strains are believed to act as powerful live vaccine vectors that are able to elicit mucosal immune responses against various pathogens. To develop efficacious and inexpensive vaccines against Mhp, the immune responses and protection induced by recombinant attenuated Salmonella vaccines based on the P42 and P97 antigens of Mhp were evaluated. In general, the oral inoculation of recombinant rSC0016(pS-P42) or rSC0016(pS-P97) resulted in strong mucosal immunity, cell-mediated immunity, and humoral immunity, which was a mixed Th1/Th2-type response. In addition, the levels of specific IL-4 and IFN-γ in the immunized mice were increased, and the proliferation of lymphocytes was also enhanced, confirming the production of a good cellular immune response. Finally, both vaccine candidate strains were able to improve the weight loss of mice after a challenge and reduce clinical symptoms, lung pathological damage, and the inflammatory cell infiltration. These results suggest that the delivery of protective antigens with recombinant attenuated Salmonella vectors may be an effective means by which to combat Mhp infection. IMPORTANCE Mhp is the main pathogen of porcine enzootic pneumonia, a highly infectious and economically significant respiratory disease that affects pigs of all ages. As the target tissue of Mhp infections are the mucosal sites of the respiratory tract, the induction of protective immunity at the mucosal tissues is the most efficient strategy by which to block disease transmission. Because the stimulation of mucosal immune responses is efficient, Salmonella-vector oral vaccines are expected to be especially useful against mucosal-invading pathogens. In this study, we expressed the immunogenic proteins of P42 and P97 with the attenuated Salmonella Choleraesuis vector rSC0016, thereby generating a low-cost and more effective vaccine candidate against Mhp by inducing significant mucosal, humoral and cellular immunity. Furthermore, rSC0016(pS-P42) effectively prevents Mhp-induced weight loss and the pulmonary inflammation of mice. Because of the effectiveness of rSC0016(pS-P42) against Mhp infection in mice, this novel vaccine candidate strain shows great potential for its use in the pig breeding industry.
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- 2022
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16. Inhibition of the LRRC8A channel promotes microglia/macrophage phagocytosis and improves outcomes after intracerebral hemorrhagic stroke
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Jing Liu, Danmin Shen, Chao Wei, Weihua Wu, Zhaoli Luo, Liye Hu, Zhongnan Xiao, Tingting Hu, Qingyu Sun, Xiaotong Wang, Yumeng Ding, Meng Liu, Miaoyi Pang, Kaiyuan Gai, Yiran Ma, Yichen Tian, Yan Yu, Peipei Wang, Yun Guan, Meng Xu, Fei Yang, and Qian Li
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Immunology and neurology ,Science - Abstract
Summary: Promoting microglial/macrophage (M/Mφ) phagocytosis accelerates hematoma clearance and improves the prognosis of intracerebral hemorrhagic stroke (ICH). Cation channels such as Piezo1 modulate bacterial clearance by regulating M/Mφ. Whether LRRC8A, an anion channel, affects M/Mφ erythrophagocytosis and functional recovery after ICH was investigated here. We found that LRRC8A is highly expressed on M/Mφ in the perihematomal region of ICH mice. Conditional knockout of Lrrc8a in M/Mφ or treatment with an LRRC8A channel blocker accelerated hematoma clearance, reduced neuronal death, and improved functional recovery after ICH. Mechanistically, the LRRC8A channel inhibition promoted M/Mφ phagocytosis by activating AMP-activated protein kinase (AMPK), thereby inducing nuclear translocation of nuclear factor-erythroid 2 related factor 2 (Nrf2) and increasing Cd36 transcription. Our findings illuminate the regulation of M/Mφ phagocytosis by the LRRC8A channel via the AMPK-Nrf2-CD36 pathway after ICH, suggesting that LRRC8A is a potential target for hematoma clearance in ICH treatment.
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- 2022
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17. MDESNet: Multitask Difference-Enhanced Siamese Network for Building Change Detection in High-Resolution Remote Sensing Images
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Jiaxiang Zheng, Yichen Tian, Chao Yuan, Kai Yin, Feifei Zhang, Fangmiao Chen, and Qiang Chen
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remote sensing ,building change detection ,Siamese network ,difference-enhanced ,multitask ,Science - Abstract
Building change detection is a prominent topic in remote sensing applications. Scholars have proposed a variety of fully-convolutional-network-based change detection methods for high-resolution remote sensing images, achieving impressive results on several building datasets. However, existing methods cannot solve the problem of pseudo-changes caused by factors such as “same object with different spectrums” and “different objects with same spectrums” in high-resolution remote sensing images because their networks are constructed using simple similarity measures. To increase the ability of the model to resist pseudo-changes and improve detection accuracy, we propose an improved method based on fully convolutional network, called multitask difference-enhanced Siamese network (MDESNet) for building change detection in high-resolution remote sensing images. We improved its feature extraction ability by adding semantic constraints and effectively utilized features while improving its recognition performance. Furthermore, we proposed a similarity measure combining concatenation and difference, called the feature difference enhancement (FDE) module, and designed comparative experiments to demonstrate its effectiveness in resisting pseudo-changes. Using the building change detection dataset (BCDD), we demonstrate that our method outperforms other state-of-the-art change detection methods, achieving the highest F1-score (0.9124) and OA (0.9874), indicating its advantages for high-resolution remote sensing image building change detection tasks.
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- 2022
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18. Association of Preoperative NANOG-Positive Circulating Tumor Cell Levels With Recurrence of Hepatocellular Carcinoma
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Yongrong Lei, Xishu Wang, Heng Sun, Yuna Fu, Yichen Tian, Ludi Yang, Jianhua Wang, and Feng Xia
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recurrence ,cancer stem cells ,hepatocellular carcinoma ,circulating tumor cells ,epithelial-mesenchymal ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
BackgroundCancer stem cells (CSCs) and Circulating tumor cells (CTCs) have been proposed as fundamental causes for the recurrence of hepatocellular carcinoma (HCC). CTCs isolated from patients with HCC illustrate a unique Nanog expression profile analysis. The aim of this study was to enhance the prediction of recurrence and prognosis of the CTC phenotype in patients with HCC by combining Nanog expression into a combined forecasting model.Subjects, Materials, and MethodsWe collected 320 blood samples from 160 patients with HCC cancer before surgery and used CanPatrol™ CTC enrichment technology and in situ hybridization (ISH) to enrich and detect CTCs and CSCs. Nanog expression in all CTCs was also determined. In addition, RT-PCR and immunohistochemistry were used to study the expression of Nanog, E-Cadherin, and N-Cadherin in liver cancer tissues and to conduct clinical correlation studies.ResultsThe numbers of EpCAM mRNA+ CTCs and Nanog mRNA+ CTCs were strongly correlated with postoperative HCC recurrence (CTC number (P = 0.03), the total number of mixed CTCS (P = 0.02), and Nanog> 6.7 (P = 0.001), with Nanog > 6.7 (P = 0.0003, HR = 2.33) being the most crucial marker. There are significant differences in the expression of Nanog on different types of CTC: most Epithelial CTCs do not express Nanog, while most of Mixed CTC and Mesenchymal CTC express Nanog, and their positive rates are 38.7%, 66.7%, and 88.7%, respectively, (P=0.0001). Moreover, both CTC (≤/> 13.3) and Nanog (≤/>6.7) expression were significantly correlated with BCLC stage, vascular invasion, tumor size, and Hbv-DNA (all P
- Published
- 2021
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19. Gender-specific contribution of cardiometabolic index and lipid accumulation product to left ventricular geometry change in general population of rural China
- Author
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Haoyu Wang, Yingxian Sun, Zhao Li, Xiaofan Guo, Shuang Chen, Ning Ye, Yichen Tian, and Lijun Zhang
- Subjects
Left ventricular geometry ,Left ventricular remodeling ,Cardiometabolic index ,Lipid accumulation product ,Obesity ,Adipose tissue ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Abstract Background Despite current interest in the unfavorable impact of cardiometabolic index (CMI) and lipid accumulation product (LAP) on diabetes and cardiovascular risk, information regarding the relation of CMI and LAP to left ventricular (LV) geometry has not been specifically addressed. We aimed to examine the hypothesis: (1) CMI and LAP represent an independent determinant of LV remodeling in general population of rural China; (2) there are gender differences in obesity-related alterations in terms of LV morphology. Methods The sample for this cross-sectional analysis included 11,258 participants (mean age 53.9 years; 54.0% females) who underwent assessment of basic metabolic and anthropometric parameters in rural areas of northeast China. Comprehensive echocardiography-defined LV geometric pattern was determined according to left ventricular mass index and relative wall thickness. Results The prevalence rate of eccentric and concentric LV hypertrophy (LVH) presented a proportional increase with elevated quartiles of CMI and LAP in a dose-response manner (all P
- Published
- 2018
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20. Landscape Pattern Theoretical Optimization of Urban Green Space Based on Ecosystem Service Supply and Demand
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Qinqin Liu, Yichen Tian, Kai Yin, Feifei Zhang, Huiping Huang, and Fangmiao Chen
- Subjects
urban green space ,ecosystem service ,supply and demand balance ,landscape pattern optimization ,Geography (General) ,G1-922 - Abstract
Assessing the supply and demand of urban green space (UGS) ecosystem services (ESs) can provide relevant insights for urban planning. This study presents an analysis method for the spatial distribution of UGS ES supply and demand at administrative unit and 1-m grid scales and directly compares the matches of ES supply and demand in spatially explicit maps at two scales. Based on the analysis results at administrative unit scale, administrative units with an unbalanced UGS ES supply and demand were divided into three types: (Ⅰ) lack of green space; (Ⅱ) unreasonable green space structure; (Ⅲ) comprehensive, and different optimization schemes were put forward. According to the analysis results at 1-m scale, the regions with an unbalanced ES supply and demand of an administrative unit were divided into the following: (1) severe ES shortage area; (2) moderate ES shortage area; (3) mild ES shortage area, and the severe ES shortage area was taken as the UGS optimization area. We take the UGS within the 5th Ring Road of Beijing as an example and propose suggestions for optimizing the UGS pattern based on the evaluation of the supply and demand of UGS carbon sequestration services and purification services for particulate matter with an aerodynamic diameter 2.5). This study provides an easy-to-use evaluation method for the spatial distribution of UGS ES supply and demand and proposes different optimization suggestions for the unbalanced area, thus playing a role in UGS construction activities and green space structure optimization.
- Published
- 2021
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21. Examining Land Use and Land Cover Spatiotemporal Change and Driving Forces in Beijing from 1978 to 2010
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Yichen Tian, Kai Yin, Dengsheng Lu, Lizhong Hua, Qianjun Zhao, and Meiping Wen
- Subjects
land use and land cover change ,object-oriented classification ,anthropogenic driving force ,Landsat ,Beijing ,Science - Abstract
Land use and land cover (LULC) datasets for Beijing in 1978, 1987, 1992, 2000 and 2010 were developed from Landsat images using the object-oriented classification approach. The relationships between social-economic, demographic and political factors and time-series LULC data were examined for the periods between 1978 and 2010. The results showed the effectiveness of using the object-oriented decision tree classification method for LULC classification with time series of Landsat images. Combined with anthropogenic driving forces, our research can effectively explain the detailed LULC change trajectories corresponding to different stages and give new insights for Beijing LULC change patterns. The results show a significant increase in forest and built-up areas, but a decrease in arable lands, due to urbanization and reforestation. Large ecological projects result in an increase of forest areas and population, and economic conditions result in urban expansion. The anthropogenic driving forces analysis results further prove that both population increase and economic development played important roles in the expansion of built-up areas. Both the qualitative and quantitative anthropogenic driving forces analysis methods were helpful for better understanding the mechanisms of LULC change.
- Published
- 2014
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22. Improved UAV Opium Poppy Detection Using an Updated YOLOv3 Model
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Jun Zhou, Yichen Tian, Chao Yuan, Kai Yin, Guang Yang, and Meiping Wen
- Subjects
uav ,opium poppy ,object detection ,yolov3 model ,deep learning ,cnn ,spatial pyramid pooling ,giou ,Chemical technology ,TP1-1185 - Abstract
Rapid detection of illicit opium poppy plants using UAV (unmanned aerial vehicle) imagery has become an important means to prevent and combat crimes related to drug cultivation. However, current methods rely on time-consuming visual image interpretation. Here, the You Only Look Once version 3 (YOLOv3) network structure was used to assess the influence that different backbone networks have on the average precision and detection speed of an UAV-derived dataset of poppy imagery, with MobileNetv2 (MN) selected as the most suitable backbone network. A Spatial Pyramid Pooling (SPP) unit was introduced and Generalized Intersection over Union (GIoU) was used to calculate the coordinate loss. The resulting SPP-GIoU-YOLOv3-MN model improved the average precision by 1.62% (from 94.75% to 96.37%) without decreasing speed and achieved an average precision of 96.37%, with a detection speed of 29 FPS using an RTX 2080Ti platform. The sliding window method was used for detection in complete UAV images, which took approximately 2.2 sec/image, approximately 10× faster than visual interpretation. The proposed technique significantly improved the efficiency of poppy detection in UAV images while also maintaining a high detection accuracy. The proposed method is thus suitable for the rapid detection of illicit opium poppy cultivation in residential areas and farmland where UAVs with ordinary visible light cameras can be operated at low altitudes (relative height < 200 m).
- Published
- 2019
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23. Opium Poppy Detection Using Deep Learning
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Xiangyu Liu, Yichen Tian, Chao Yuan, Feifei Zhang, and Guang Yang
- Subjects
remote sensing ,object detection ,opium poppy ,deep learning ,single shot multibox detector (SSD) ,Lao PDR ,Science - Abstract
Opium poppies are a major source of traditional drugs, which are not only harmful to physical and mental health, but also threaten the economy and society. Monitoring poppy cultivation in key regions through remote sensing is therefore a crucial task; the location coordinates of poppy parcels represent particularly important information for their eradication by local governments. We propose a new methodology based on deep learning target detection to identify the location of poppy parcels and map their spatial distribution. We first make six training datasets with different band combinations and slide window sizes using two ZiYuan3 (ZY3) remote sensing images and separately train the single shot multibox detector (SSD) model. Then, we choose the best model and test its performance using 225 km2 verification images from Lao People’s Democratic Republic (Lao PDR), which exhibits a precision of 95% for a recall of 85%. The speed of our method is 4.5 km2/s on 1080TI Graphics Processing Unit (GPU). This study is the first attempt to monitor opium poppies with the deep learning method and achieve a high recognition rate. Our method does not require manual feature extraction and provides an alternative way to rapidly obtain the exact location coordinates of opium poppy cultivation patches.
- Published
- 2018
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24. Device-Free Human Tracking and Gait Recognition Based on the Smart Speaker.
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Yichen Tian, Yunliang Wang, Yufan Wang, Xinyu Tong, Xiulong Liu, and Wenyu Qu
- Published
- 2024
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- View/download PDF
25. NNE-Tracking: A Neural Network Enhanced Framework for Device-Free Wi-Fi Tracking.
- Author
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Xinyu Tong, Weiping Ge, Yichen Tian, Zijuan Liu, Xiulong Liu, and Wenyu Qu
- Published
- 2024
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26. WSTrack: A Wi-Fi and Sound Fusion System for Device-free Human Tracking.
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Yichen Tian, Yunliang Wang, Ruikai Zheng, Xiulong Liu, Xinyu Tong, and Keqiu Li
- Published
- 2023
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27. CrossTrack: Device-Free Cross-Link Tracking With Commodity Wi-Fi.
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Weiping Ge, Yichen Tian, Xiulong Liu, Xinyu Tong, Wenyu Qu, Zhenzhe Zhong, and Haojie Chen
- Published
- 2023
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28. A Magnetic Gradient Tensor Based Method for UXO Detection on Movable Platform.
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Xiaoping Zheng, Yichen Tian, and Bin Wang
- Published
- 2023
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29. Association of Preoperative NANOG-Positive Circulating Tumor Cell Levels With Recurrence of Hepatocellular Carcinoma.
- Author
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Yongrong Lei, Xishu Wang, Heng Sun, Yuna Fu, Yichen Tian, Ludi Yang, Jianhua Wang, and Feng Xia
- Subjects
CANCER stem cells ,IN situ hybridization ,LIVER cancer ,RADIOTHERAPY ,ONCOLOGIC surgery ,CANCER invasiveness ,HEPATOCELLULAR carcinoma - Abstract
Background: Cancer stem cells (CSCs) and Circulating tumor cells (CTCs) have been proposed as fundamental causes for the recurrence of hepatocellular carcinoma (HCC). CTCs isolated from patients with HCC illustrate a unique Nanog expression profile analysis. The aim of this study was to enhance the prediction of recurrence and prognosis of the CTC phenotype in patients with HCC by combining Nanog expression into a combined forecasting model. Subjects, Materials, and Methods: We collected 320 blood samples from 160 patients with HCC cancer before surgery and used CanPatrol™ CTC enrichment technology and in situ hybridization (ISH) to enrich and detect CTCs and CSCs. Nanog expression in all CTCs was also determined. In addition, RT-PCR and immunohistochemistry were used to study the expression of Nanog, E-Cadherin, and N-Cadherin in liver cancer tissues and to conduct clinical correlation studies. Results: The numbers of
EpCAM mRNA+ CTCs andNanog mRNA+ CTCs were strongly correlated with postoperative HCC recurrence (CTC number (P = 0.03), the total number ofmixed CTCS (P = 0.02), and Nanog> 6.7 (P = 0.001), with Nanog > 6.7 (P = 0.0003, HR = 2.33) being the most crucial marker. There are significant differences in the expression of Nanog on different types of CTC:most Epithelial CTCs do not express Nanog, whilemost of Mixed CTC and Mesenchymal CTC express Nanog, and their positive rates are 38.7%, 66.7%, and 88.7%, respectively, (P=0.0001). Moreover, both CTC (≤/> 13.3) and Nanog (≤/>6.7) expression were significantly correlated with BCLC stage, vascular invasion, tumor size, and Hbv-DNA (all P < 0.05). In the young group and the old group, patients with higher Nanog expression had a higher recurrence rate. (P < 0.001). Conclusions: The number of Nanog-positive cells showed positive correlation with the poor prognosis of HCC patients. The detection and analysis of CTC markers (EpCAM and CK8, 18, CD45 Vimentin, Twist and 19) and CSCs markers (NANOG) are of great value in the evaluation of tumor progression. [ABSTRACT FROM AUTHOR]- Published
- 2024
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- View/download PDF
30. A machine learning method for improving liver cancer staging.
- Author
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Zhengyun Zhao, Yichen Tian, Zheng Yuan 0002, Peng Zhao, Feng Xia 0010, and Sheng Yu 0002
- Published
- 2023
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31. Detecting fake news by exploring the consistency of multimodal data.
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Junxiao Xue, Yabo Wang, Yichen Tian, Yafei Li, Lei Shi 0001, and Lin Wei
- Published
- 2021
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32. Clinico-characteristics of patients which correlated with preferable treatment outcomes in immunotherapy for advanced hepatocellular carcinoma: a systematic review and meta-analysis.
- Author
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Yani Wang, Wanyee Lau, Yafei Li, Yichen Tian, Yongrong Lei, Jianhua Wang, and Feng Xia
- Abstract
Background and aims: Hepatocellular carcinoma (HCC) is the third-most lethal malignant tumor worldwide. The rapid development of immunotherapy utilizing immune checkpoint inhibitors for advanced HCC patients has been witnessed in recent years, along with numerous randomized clinical trials demonstrating the survival benefits for these individuals. This systematic review and meta-analysis aimed to identify specific clinico-pathological characteristics of advanced HCC patients that may lead to preferable responses to immunotherapy in terms of overall survival (OS), progression-free survival (PFS), and objective response rate (ORR). Methods: The included clinical trials were retrieved from PubMed, Embase, the Cochrane library, and the Web of Science databases published in English between 1 January 2002 and 20 October 2022. A systematic review and meta-analysis for first-line and second-line phase II/III studies were conducted on immunotherapy for patients with advanced HCC by using OS as the primary outcome measure, and PFS and ORR as the secondary outcome measures to obtain clinico-pathological characteristics of patients which might be preferable responses to programmed death-1 (PD-1) and programmed cell death-Ligand 1 (PD-L1) inhibitors. Toxicity and specific treatment-related adverse events (TRAEs) were also determined. Results: After screening 1392 relevant studies, 12 studies were included in this systematic review and meta-analysis to include 5948 patients. Based on the analysis of interaction, the difference in OS after first-line immunotherapy between the subgroups of viral hepatitis [hazard ratio (HR) =0.73 vs 0.87, P for interaction= 0.02] and macrovascular invasion and/or extrahepatic spread (HR=0.73 vs 0.89, P for interaction= 0.02) were significant. The difference in PFS between the subgroups of viral hepatitis was highly significant (pooled HR= 0.55 vs 0.81, P for interaction= 0.007). After second-line immunotherapy, the difference in ORR between the subgroups of Barcelona Clinic Liver Cancer was significant (pooled ES= 0.12 vs 0.23, P for interaction= 0.04). Compared with PD-L1 inhibitors, PD-1 inhibitors may have a higher probability to cause TRAEs. Diarrhea, increased aspartate aminotransferase, and hypertension were the top three TRAEs. Conclusions: This systematic review and meta-analysis represents the first pilot study aimed at identifying crucial clinicopathological characteristics of patients with advanced HCC that may predict favorable treatment outcomes in terms of OS, PFS, and ORR to immunotherapy. Findings suggest that patients with viral hepatitis positivity (especially hepatitis B virus) and macrovascular invasion and/or extrahepatic spread may benefit more in OS when treated with PD-1/PD-L1 immune checkpoint inhibitors. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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33. Engineered Bacterial Outer Membrane Vesicles with Lipidated Heterologous Antigen as an Adjuvant-Free Vaccine Platform for Streptococcus suis
- Author
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Quan Li, Guodong Zhou, Xia Fei, Yichen Tian, Shifeng Wang, and Huoying Shi
- Subjects
Ecology ,Applied Microbiology and Biotechnology ,Food Science ,Biotechnology - Abstract
Bacterial outer membrane vesicles (OMVs) have become a promising vaccine platform due to their excellent built-in adjuvanticity properties. However, the location and amount of the expression of the heterologous antigen in the OMVs delivered by the genetic engineering strategies should be optimized.
- Published
- 2023
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34. Reduced graphene oxide/graphitic carbon nitride composite for piezocatalysis and piezocatalytic activation of persulfate to degrade sulfamethoxazole
- Author
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Yichen Tian, Minghao Sui, Yanning Lv, and Xinyuan Lv
- Subjects
Process Chemistry and Technology ,Safety, Risk, Reliability and Quality ,Waste Management and Disposal ,Biotechnology - Published
- 2023
- Full Text
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35. Crop Growth Monitoring Based on the MODIS Data.
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Wenbo Xu 0004, Yong Zhang 0052, Yichen Tian, Jianxi Huang, and Binbin He
- Published
- 2006
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36. Comparison of land cover product in Sichuan province of China.
- Author
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Wenbo Xu 0004, Yichen Tian, Jianxi Huang, Yong Zhang 0052, and Yuancheng Sun
- Published
- 2005
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37. A method of estimating crop acreage in large-scale by unmixing of MODIS data.
- Author
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Wenbo Xu 0004, Jianxi Huang, Yichen Tian, Yong Zhang 0052, and Yuancheng Sun
- Published
- 2005
- Full Text
- View/download PDF
38. Synergy of multitemporal Radarsat SAR and Landsat ETM data for extracting agricultural crops structure.
- Author
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Wenbo Xu 0004, Bingfang Wu, Yichen Tian, Jianxi Huang, and Yong Zhang 0052
- Published
- 2004
- Full Text
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39. Crop drought monitoring using serial NDVI & NDWI in Northern China.
- Author
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Chenglin Liu, Bingfang Wu, Yichen Tian, Wenbo Xu 0004, and Jianxi Huang
- Published
- 2004
- Full Text
- View/download PDF
40. Spectral Discrimination of Opium Poppy Using Field Spectrometry.
- Author
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Kun Jia, Bingfang Wu, Yichen Tian, Qiangzi Li, and Xin Du 0004
- Published
- 2011
- Full Text
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41. WWP2 regulates SIRT1‐STAT3 acetylation and phosphorylation involved in hypertensive angiopathy
- Author
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Saien Lu, Yichen Tian, Naijin Zhang, Yingxian Sun, Shilong You, Ying Zhang, and Liu Cao
- Subjects
Male ,STAT3 Transcription Factor ,0301 basic medicine ,Vascular smooth muscle ,Ubiquitin-Protein Ligases ,Myocytes, Smooth Muscle ,post‐translational modification ,WWP2 ,Muscle, Smooth, Vascular ,Angiopathy ,STAT3 ,Mice ,03 medical and health sciences ,SIRT1 ,0302 clinical medicine ,Sirtuin 1 ,Cell Movement ,Renin–angiotensin system ,medicine ,Animals ,Phosphorylation ,Cell Proliferation ,Mice, Knockout ,biology ,Chemistry ,Angiotensin II ,hypertensive angiopathy ,Acetylation ,Original Articles ,Cell Biology ,medicine.disease ,Ubiquitin ligase ,Cell biology ,030104 developmental biology ,030220 oncology & carcinogenesis ,Hypertension ,Knockout mouse ,biology.protein ,Molecular Medicine ,Original Article - Abstract
WWP2 is a HECT‐type E3 ubiquitin ligase that regulates various physiological and pathological activities by binding to different substrates, but its function and regulatory mechanism in vascular smooth muscle cells (VSMCs) are still unknown. Here, we clarified the role of WWP2 in the regulation of SIRT1‐STAT3 and the impact of this regulatory process in VSMCs. We demonstrated that WWP2 expression was significantly increased in angiotensin II‐induced VSMCs model. Knockdown of WWP2 significantly inhibited angiotensin II‐induced VSMCs proliferation, migration and phenotypic transformation, whereas overexpression of WWP2 had opposite effects. In vivo experiments showed that vascular smooth muscle‐specific WWP2 knockout mice significantly relieved angiotensin II‐induced hypertensive angiopathy. Mechanistically, mass spectrometry and co‐immunoprecipitation assays identified that WWP2 is a novel interacting protein of SIRT1 and STAT3. Moreover, WWP2 formed a complex with SIRT1‐STAT3, inhibiting the interaction between SIRT1 and STAT3, then reducing the inhibitory effect of SIRT1 on STAT3, ensuing promoting STAT3‐K685 acetylation and STAT3‐Y705 phosphorylation in angiotensin II‐induced VSMCs and mice. In conclusion, WWP2 modulates hypertensive angiopathy by regulating SIRT1‐STAT3 and WWP2 suppression in VSMCs can alleviate hypertensive angiopathy vitro and vivo. These findings provide new insights into the treatment of hypertensive vascular diseases.
- Published
- 2020
- Full Text
- View/download PDF
42. Septin4 Prevents PDGF-BB-induced HAVSMC Phenotypic Transformation, Proliferation and Migration by Promoting SIRT1-STAT3 Deacetylation and Dephosphorylation
- Author
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Naijin Zhang, Ying Zhang, Yichen Tian, Yingxian Sun, Shilong You, Liu Cao, and Saien Lu
- Subjects
Male ,STAT3 Transcription Factor ,Vascular smooth muscle ,Cell Survival ,Blotting, Western ,Becaplermin ,Applied Microbiology and Biotechnology ,Cell Line ,Dephosphorylation ,STAT3 ,03 medical and health sciences ,Mice ,SIRT1 ,Sirtuin 1 ,Cell Movement ,Animals ,Immunoprecipitation ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,030304 developmental biology ,Cell Proliferation ,0303 health sciences ,Gene knockdown ,Septin4 ,biology ,Chemistry ,Cell Biology ,Atherosclerosis ,Phenotype ,Mice, Mutant Strains ,Cell biology ,Transformation (genetics) ,Acetylation ,biology.protein ,Platelet-derived growth factor receptor ,Septins ,Developmental Biology ,Research Paper ,Signal Transduction - Abstract
SIRT1 and STAT3 are key to human aortic vascular smooth muscle cells (HAVSMCs) proliferation, migration and phenotypic transformation, but the regulatory mechanism of SIRT1-STAT3 in this process is still unclear. Septin4 is a cytoskeleton-related protein that regulates oxidative stress-vascular endothelial injury. However, the role and underlying mechanism of Septin4 in atherosclerosis remains unknown. Here, we revealed the role and mechanism of Septin4 in regulating SIRT1-STAT3 in atherosclerosis. We determined that the expression of Septin4 were markedly increased in Apoe-/- atherosclerosis mice and PDGF-BB-induced HAVSMCs. Knockdown of Septin4 significantly increased PDGF-BB-induced HAVSMCs proliferation, migration and phenotypic transformation, while overexpression of Septin4 had the opposite effects. Mechanically, co-immunoprecipitation results demonstrated that Septin4 was a novel interacting protein of STAT3 and SIRT1. Septin4 formed a complex with SIRT1-STAT3, enhancing the interaction between SIRT1 and STAT3, ensuing promoting SIRT1-regulated STAT3-K685 deacetylation and STAT3-Y705 dephosphorylation, which inhibited PDGF-BB-induced HAVSMCs proliferation, migration and phenotype transformation. Therefore, our findings provide novel insights into the prevention and treatment of atherosclerosis.
- Published
- 2020
43. Seismic interpretation and geological evaluation of hydrocarbon source rocks in volcanic-rich continental lacustrine rift basins: A case study of the Lower Cretaceous Yingcheng Formation from the Changling Fault Depression in the Songliao Basin, NE China
- Author
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Zihan Zhang, Hongyu Wang, Xiongbing Yang, Xinyan Su, Yichen Tian, Jing Wang, and Nuo Shi
- Published
- 2023
- Full Text
- View/download PDF
44. Phytate-based transparent and waterproof intumescent flame-retardant coating for protection of wood
- Author
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Yichen Tian, Chuyi Wang, Yuanfang Ai, Longcheng Tang, and Kun Cao
- Subjects
General Materials Science ,Condensed Matter Physics - Published
- 2023
- Full Text
- View/download PDF
45. Field survey framework strategy of soil and water conservation practices based on geoinformatic technology.
- Author
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Weifeng Zhou, Bingfang Wu, Wenbo Huang, and Yichen Tian
- Published
- 2005
- Full Text
- View/download PDF
46. A segmentation and classification approach of land cover mapping using Quick Bird image.
- Author
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Wenbo Xu 0004, Bingfang Wu, Jianxi Huang, Yong Zhang 0052, and Yichen Tian
- Published
- 2004
- Full Text
- View/download PDF
47. A ruled-based approach to evaluate soil loss at catchments level in Miyun hilly region.
- Author
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Bingfang Wu, Yichen Tian, Wenting Xu, Jianxi Huang, and Wenbo Xu 0004
- Published
- 2004
- Full Text
- View/download PDF
48. An effective field method of crop proportion survey in China based on GVG integrated system.
- Author
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Yichen Tian, Bingfang Wu, Wenbo Xu 0004, Jianxi Huang, and Wenting Xu
- Published
- 2004
- Full Text
- View/download PDF
49. Mapping plant diversity of broad-leaved forest ecosystem using Landsat TM data.
- Author
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Wenting Xu, Bingfang Wu, Yichen Tian, and Yuan Zeng
- Published
- 2004
- Full Text
- View/download PDF
50. Spatial pattern of soil and water loss and its affecting factors analysis in the upper basin of Miyun reservoir.
- Author
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Bingfang Wu, Yuemin Zhou, Jianxi Huang, Yichen Tian, and Wenbo Huang
- Published
- 2004
- Full Text
- View/download PDF
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