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Objectives: Since December 2019, acute respiratory disease due to 2019 novel coronavirus emerged in Wuhan city and rapidly spread throughout China. Real-time RT-PCR is widely deployed in diagnostic virology. However, the positive detection rates of RT-PCR are only 30% to 50%. Therefore, we propose a simple strategy for rapidly and sensitively detecting the IgM/IgG antibody against 2019-nCoV using a colloidal gold-based immunochromatographic strip test.Methods A total of 41 clinically 2019-nCoV suspected cases (23 males and 18 females) were enrolled. The sensitivity of colloidal gold-based immunochromatographic strip test and of RT-PCR were compared and evaluated. McNemar’s test was used to compare the detection rate of both assays (PResults: The Antibody was detected in 63.4% (26/41) of blood specimens using the assay. In contrast, the 2019-nCoV was detected in 46.3% (19/41) of nasal and pharyngeal swab specimens using the RT-PCR assays. The detection rate obtained by this assay was markedly higher than that obtained by the RT-PCR assays (P=0.039)Conclusion: This detection assay exhibits a higher detection sensitivity than RT-PCR. More important, the assay shows the benefits of easy operation and setup. We believe that the sensitive and time-saving approach may be used as an auxiliary diagnostic tool for 2019-nCoV detection and virus screening and confirmation.

Objectives The blaKPC gene is rarely reported in Citrobacter koseri. Here we report the first draft genome sequence of a blaKPC-2-carrying C. koseri isolate from a patient with diarrhoea. Methods Transferability of the blaKPC-2-bearing plasmid was determined by the filter mating method. The whole genome sequence of C. koseri L168 was determined using an Illumina HiSeq platform. The genome was de novo assembled using Velvet 1.2.10. Acquired antimicrobial resistance genes and plasmid replicons were identified using ResFinder 2.1 and PlasmidFinder 1.3, respectively. Results Antimicrobial susceptibility testing (AST) showed that C. koseri L168 was resistant to multiple antibiotics but was susceptible to ciprofloxacin, gentamicin, tobramycin, amikacin, tigecycline and colistin. A KPC-2-harbouring plasmid was conjugative and the transconjugants conferred increased resistance to carbapenems confirmed by conjugation experiments and AST. In silico analysis revealed the presence of the β-lactam resistance genes blaKPC-2 and blaMAL-1. Additionally, plasmids of incompatibility groups IncFII and IncX4 were identified in the genome by PlasmidFinder. BLAST analysis revealed that blaKPC-2 was located on a Tn3 transposon element in C. koseri L168 with the conserved linear structure ISKpn27–blaKPC-2–ΔISKpn6–korC–klcA. Conclusions To our knowledge, this is only the second report of C. koseri producing KPC-2, and we report the first draft genome sequence of a blaKPC-2-carrying C. koseri isolate from a patient with diarrhoea in China. This work may facilitate our understanding of the pathogenesis, multidrug resistance mechanisms and genomic features of this species. Further monitoring of bacteria carrying carbapenemase genes in patients’ gut microbiota is warranted.

Staphylococcus microti DSM 22147 was isolated from viscera of common voles ( Microtus arvalis Pallas) with generalized Brucella microti infection in the Czech Republic. To the best of our knowledge, the genome sequence of the species S. microti has not been previously studied. The complete genome sequence of strain DSM 22147 includes a genome of 2,381,859 bp (38.0% GC content) without any plasmids.