Search

Your search keyword '"Yi-yun Wei"' showing total 3 results
Start Over Author "Yi-yun Wei"
3 results on '"Yi-yun Wei"'

1. Identification and tissue distribution of odorant binding protein genes in Harmonia axyridis (Coleoptera: Coccinellidae)

Author

Cheng QU, Ran WANG, Wu-nan CHE, Feng-qi LI, Hai-peng ZHAO, Yi-yun WEI, Chen LUO, and Ming XUE

Subjects
odorant-binding proteins, Harmonia axyridis, expression profile, phylogenetic tree, Agriculture (General), S1-972
Abstract

The olfactory system of insects is crucial in modulating behaviors such as host seeking, mating, and oviposition. Odorant-binding proteins (OBPs) are involved in semiochemical recognition. OBPs recognize and bind odorants and transport them to odorant receptors located in olfactory neurons. Harmonia axyridis (Coleoptera: Coccinellidae) is a widely used predacious biological control agent for many agricultural and forestry pests. This study identified 19 OBPs in H. axyridis based on the antennal and whole-body transcriptomes of adults and obtained all the full-length open reading frames, including 11 ‘Classic’ OBPs, 7 ‘Minus-C’ OBPs and 1 ‘Plus-C’ OBP. They encoded 125 to 241 amino acid proteins with molecular weights ranging from 13.75 to 27.75 kDa and isoelectric points ranging from 4.15 to 8.80. Phylogenetic analyses were used to study the relationships between H. axyridis OBPs and OBPs from other species of Coleoptera. Quantitative real-time PCR (qPCR) analysis showed that HaxyOBP2, 3, 5, 8, 10, 12, 13, 14, and 15 were highly expressed in antennae of both adult females and males. Moreover, HaxyOBP2, 3, 5, 12, and 15 were more abundantly expressed in antennae than other body parts, while HaxyOBP13 and HaxyOBP14 were expressed predominantly, and at similar levels, in the head and antennae. The other OBP genes were highly expressed in non-olfactory tissues including the thorax, abdomen, legs, and wings. These results provide valuable information for further study of H. axyridis olfaction, which may ultimately enhance its use as a biocontrol agent.

Published
2021
Full Text
View/download PDF

2. Amplicon sequencing detects mutations associated with pyrethroid resistance in <scp> Bemisia tabaci </scp> (Hemiptera: Aleyrodidae)

Author

Ran Wang, Yi-yun Wei, Cheng Qu, Chen Luo, and Fang Guan

Subjects
0106 biological sciences, China, Insecticides, Population, medicine.disease_cause, 01 natural sciences, Hemiptera, Insecticide Resistance, chemistry.chemical_compound, symbols.namesake, Pyrethrins, medicine, Pyrethroid resistance, Animals, Allele, education, Genetics, Sanger sequencing, Mutation, education.field_of_study, Pyrethroid, biology, General Medicine, biology.organism_classification, 010602 entomology, chemistry, Insect Science, Amplicon sequencing, symbols, Agronomy and Crop Science, 010606 plant biology & botany
Abstract

Background Bemisia tabaci (Gennadius) is a major damaging agricultural pest that exhibits high resistance to pyrethroid insecticides. L925I (TTA to ATA) and T929V (ACT to GTT) mutations in the para-type voltage-gated sodium channel (VGSC) are associated with resistance of B. tabaci to pyrethroids. Amplicon sequencing is a reliable and highly efficient method to detect the frequency of mutations linked with insecticide resistance. Results Similar frequencies of L925I and T929V mutations were obtained by amplicon sequencing and Sanger sequencing (L925I: 0.3548 vs 0.3619; T929V: 0.6140 vs 0.6381) with overlap of 95% confidence interval in the SX population of B. tabaci. In five populations of B. tabaci from China, the maximum and minimum frequencies of the two mutations were found in the LN (L925I: 0.1126; T929V: 0.8834) and JS (L925I: 0.8776; T929V: 0.1166) populations by amplicon sequencing. However, there was no significant difference in frequencies between the L925I and T929V mutations. The sum frequency of L925I and T929V exceeded 0.9688 in all populations. In addition, a combining mutation, L925 + T929V (L925I and T929V located in same allele), was found in five populations by amplicon sequencing even though its highest frequency was only 0.0157. Conclusion We established an efficient approach for detecting frequency of mutation by amplicon sequencing. The frequencies of L925I and T929V in VGSC associated with pyrethroid resistance were detected in this study, which could provide foundational data for resistance management of B. tabaci. © 2021 Society of Chemical Industry.

Published
2021
Full Text
View/download PDF

3. [Chinese version of SF-36 in the quality of life assessment among community-dwelling elders]

Author

Yi-yun, Wei, Yan, Yan, Duo-lao, Wang, Yan-fang, Guo, Lei, Zhang, and Zhen-qiu, Sun

Subjects
Aged, 80 and over, Male, Surveys and Questionnaires, Quality of Life, Humans, Reproducibility of Results, Female, Community Health Services, Middle Aged, Geriatric Assessment, Sampling Studies, Aged
Abstract

To obtain scores in a community-dwelling population over 60 using the SF-36, to assess the reliability and validity of this general health questionnaire, and to analyze the difference in dimension scores among the elderly Chinese in Changsha.We randomly selected 602 elders, aged 60 to 91 years, in multi-phases. All the subjects had resided in Changsha for at least one year. The reliability of the SF-36 was assessed by split-half reliability and Cronbach's alpha coefficient and the validity through factor analysis and correlation analysis, etc. The dimension scores of different people were obtained by analysis of variance and independent-samples t-test.The split-half reliability was 0.72 and the Cronbach's alpha coefficients of all the 8 dimensions were more than 0.8; the Pearson correlate coefficients of all the items to their dimensions were more than 0. 59. SF-36 contained 8 domains and 2 summary scales in the factor analysis. Health-related quality was different in different elders.The SF-36 is practical in studying the quality of life among community-dwelling elders.

Published
2006

Catalog

Books, media, physical & digital resources
See catalog results