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3 results on '"Yi-jie Wen"'

1. Transcription level differences in Taxus wallichiana var. mairei elicited by Ce3+, Ce4+ and methyl jasmonate

Author

Na Han, Wen-ji Geng, Jian Li, Shu-ting Liu, Jie Zhang, Yi-jie Wen, Huai-hua Xu, Meng-yuan Li, Yan-ru Li, and Pei-pei Han

Subjects
T. mairei, transcriptome, taxol biosynthesis, cerium, MJ, Plant culture, SB1-1110
Abstract

Taxol is a precious and effective anticancer drug. Cerium and methyl jasmonate (MJ) have been shown to increase the yield of taxol in taxus cells. However, the mechanisms of cerium-mediated and MJ-mediated taxol biosynthesis remain unknown. RNA-Seq was applied to study the overall regulation mechanism of cerium and MJ on taxol biosynthesis and analyze the differences among T. mairei cells elicited by Ce3+, Ce4+ and MJ on transcriptional level . Using sequence homology, 179 unigenes were identified as taxol synthesis genes. Under the condition of 100 μM MJ, taxol synthesis genes were up-regulated. Notably, taxol synthesis genes were down-regulated expression at 1 mM Ce3+ and 1 mM Ce4+. Differential expression genes involved in some related functions were analyzed, such as MAPK signaling pathway and plant-pathogen interaction. Sequence alignment and phylogenetic analysis of nine differentially expressed WRKYs in our data were carried out.

Published
2022
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2. Transcription level differences in Taxus wallichiana var. mairei elicited by Ce3+, Ce4+ and methyl jasmonate.

Author

Na Han, Wen-ji Geng, Jian Li, Shu-ting Liu, Jie Zhang, Yi-jie Wen, Huai-hua Xu, Meng-yuan Li, Yan-ru Li, and Pei-pei Han

Subjects
JASMONATE, PACLITAXEL, SEQUENCE alignment, ANTINEOPLASTIC agents, CERIUM
Abstract

Taxol is a precious and effective anticancer drug. Cerium and methyl jasmonate (MJ) have been shown to increase the yield of taxol in taxus cells. However, the mechanisms of cerium-mediated and MJ-mediated taxol biosynthesis remain unknown. RNA-Seq was applied to study the overall regulation mechanism of cerium and MJ on taxol biosynthesis and analyze the differences among T. mairei cells elicited by Ce3+, Ce4+ and MJ on transcriptional level. Using sequence homology, 179 unigenes were identified as taxol synthesis genes. Under the condition of 100 mM MJ, taxol synthesis genes were up-regulated. Notably, taxol synthesis genes were down-regulated expression at 1 mM Ce3+ and 1 mM Ce4+. Differential expression genes involved in some related functions were analyzed, such as MAPK signaling pathway and plantpathogen interaction. Sequence alignment and phylogenetic analysis of nine differentially expressed WRKYs in our data were carried out. [ABSTRACT FROM AUTHOR]

Published
2022
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3. [Effect of naringin on oxidative stress and endoplasmic reticulum stress in diabetic cardiomyopathy]

Author

Yu-Fei, Zhang, Na-Na, Meng, Hou-Zhong, Li, Yi-Jie, Wen, Jie-Ting, Liu, Chun-Lei, Zhang, Xiao-Huan, Yuan, and Xiu-Dong, Jin

Subjects
Male, Cardiotonic Agents, Diabetic Cardiomyopathies, Superoxide Dismutase, Apoptosis, Endoplasmic Reticulum Stress, Diabetes Mellitus, Experimental, Rats, Rats, Sprague-Dawley, Oxidative Stress, Malondialdehyde, Flavanones, Animals, Caspase 12, Heat-Shock Proteins, Transcription Factor CHOP
Abstract

To explore the protective effect of naringin(Nar) on the injury of myocardium tissues induced by streptozotocin(STZ) in diabetic rats and the relationship with oxidative stress and endoplasmic reticulum stress(ERS)， the male SD rats were intraperitoneally injected with streptozotocin(STZ， 60 mg·kg⁻¹) to establish the diabetic rat model and then randomly divided into the type 1 diabetic rat group(T1DR)， the low-dose Nar group(Nar25)， the middle-dose Nar group(Nar50) and the high-dose Nar group(Nar100). The normal rats were designed as control group(Con). Nar25， Nar50， Nar100 groups were orally administered with Nar at the doses of 25.0， 50.0， 100.0 mg·kg⁻¹ per day， respectively， while the normal group and the T1DR group were orally administered with saline. At the 8th week after treatment， fasting plasma glucose and heart mass index were measured. The pathological changes in myocardial tissues were observed by microscope. The cardiac malondialdehyde(MDA) level and superoxide dismutase(SOD) activities were measured. The gene and protein expressions of glucose-regulated protein 78(GRP78)， C/EBP homologous protein(CHOP)， cysteinyl aspartate-specific proteinase 12(caspase 12) were detected by qRT-PCR and Western blot. According to the results， compared with control group， the myocardial structure was damaged， the content of MDA was increased， while the activities of SOD were decreased(

Published
2017

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