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The article in BJU International discusses cystinuria, a hereditary disease characterized by increased cystine in urine leading to kidney stones. The study explores adeno-associated virus (AAV)-based gene therapy for cystinuria in mice with Slc7a9 mutation, showing promising results in reducing stone volume. The research suggests that AAV9 effectively transduces proximal tubules and may offer a potential treatment for cystinuria management. The study was supported by various funding sources and involved contributions from multiple authors. [Extracted from the article]

Background and Objective: We explore molecular and metabolic pathways involved in interstitial cystitis (IC) with integrating multi‐omics analysis for identifying potential diagnostic and therapeutic targets. Methods: Mouse models of IC/bladder pain syndrome (BPS) were established by intraperitoneal injection of cyclophosphamide and bladder tissue samples were collected for metabolomics and transcriptome analysis. Results: We found a total of 82 and 145 differential metabolites in positive ion modes and negative ion modes, respectively. Glycerophospholipid metabolism, choline metabolism in cancer, and nucleotide metabolism pathways were significantly enriched in the IC/BPS group. Transcriptome analysis demonstrated that 1069 upregulated genes and 1087 downregulated genes were detected. Importantly, the stronger enrichment for cell cycle pathway was observed in IC/BPS than that in normal bladder tissue, which may be involved in the process of bladder remodeling. Moreover, the inflammatory response and inflammatory factors related pathways were enriched in the IC/BPS group. Conclusions: Our findings provide critical directions for further exploration of the molecular pathology underlying IC/BPS. [ABSTRACT FROM AUTHOR]

Phosphatase and tensin homolog‐long (PTEN‐L) is a translational isoform of PTEN, which exists in both intracellular and extracellular locations. Previous studies demonstrated that PTEN‐L could inhibit oncogenesis due to its lipid phosphatase activity. However, recent studies found that PTEN‐L could promote the proliferation of some types of cancer cells. Moreover, as a protein phosphatase, PTEN‐L can suppress mitophagy by counteracting PTEN‐induced putative kinase protein 1 (PINK1)‐Parkin‐mediated ubiquitin phosphorylation, namely, PTEN‐L is critical for exploring the mitophagy progression and the treatment of mitochondrial diseases. Accounting for the critical functions of PTEN‐L, its antibody can be used for the treatment or prognosis of tumors and mitochondrial diseases. Currently, the commercial antibody of PTEN‐L is not available. In our study, the recombinant PTEN‐L protein was expressed in Escherichia coli BL21 and used as an antigen to immunize Japan's big‐eared white rabbit for the preparation of polyclonal antibody. The PTEN‐L protein can be captured by PTEN‐L antibody specifically and effectively. Taken together, a PTEN_L antibody is a valuable tool for further exploring the function of PTEN‐L in oncogenesis and mitochondrial diseases, and it would be a new choice for the prognosis or treatment of cancer and mitochondrial diseases. [ABSTRACT FROM AUTHOR]

Background: It is unclear whether the neurovascular bundle (NVB) sparing could improve post‐operative urinary continence and potency. Furthermore, concern remains regarding the impact of nerve‐sparing (NS) radical cystectomy (RC) on oncological outcomes. Objectives: The primary objective of this meta‐analysis was to evaluate whether in men undergoing NS RC could improve post‐operative urinary continence and potency. The secondary objective was to assess whether NS RC could compromise the oncological control. Materials and methods: A systematic search of the PubMed and Web of Science was performed in February 2020, yielding 1446 unique records. A total of 13 comparative cohort studies were included. Risk of bias in each study was assessed separately by two authors using the Newcastle‐Ottawa Scale (NOS). Results: Data from 921 participants in 12 studies were synthesized in the present meta‐analysis. Meta‐analysis revealed that NS compared with non–nerve sparing (NNS) results in improved post‐operative potency, daytime continence, and nocturnal continence. RRs were 9.35 (P <.00001) in potency, 1.11 (P =.045) in daytime continence, and 1.33 (P =.002) in nocturnal continence, respectively. Furthermore, no differences were found in the included studies reporting oncological outcomes. RRs were 0.88 (P =.61) in local and/or distant recurrence between two groups. A sensitivity analysis of prospective studies indicated consistent results. Discussion and conclusion: This meta‐analysis indicates that NS RC can improve post‐operative potency, and daytime and nocturnal urinary continence, without compromising oncological control, compared with NNS RC in men. [ABSTRACT FROM AUTHOR]

Background: The benefits of vitamin E (VE) for multiple health outcomes have been well evaluated in many recent studies., Objective: The purpose of this umbrella review was to conduct a systematic evaluation of the possible associations between VE intake and various health outcomes., Methods: We systematically searched various databases, such as PubMed, Embase, and the Web of Science, to identify related meta-analyses of observational studies and randomized trials. We estimated the effect size of each association by using the random or fixed effects models and the 95% confidence intervals. We used standard approaches to evaluate the quality of the articles (AMSTAR) and classified the evidence into different levels of quality (GRADE)., Results: A total of 1,974 review articles were searched, and 27 articles with 28 health outcomes were yielded according to our exclusion criteria. The intake of VE was inversely associated with the risk of breast cancer, lung cancer, esophageal cancer, gastric cancer, pancreatic cancer, kidney cancer, bladder cancer, cervical neoplasms, cardiovascular disease, Parkinson's disease, depression, age-related cataracts, metabolic syndrome, and fracture. Overall, most of the quality of the evidence was low or very low. Three outcomes (stroke, age-related cataracts, obesity) were identified as having a "moderate" level of quality. The AMSTAR scores for all health outcomes ranged from 5 to 10., Conclusion: Our study revealed that VE intake is beneficially related to multiple health outcomes. However, future studies on recommended doses and recommended populations of VE are also needed., Systematic Review Registration: http://www.crd.york.ac.uk/PROSPERO/, identifier: CRD42022339571., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Zhang, Yi, Li, Zheng, Xu, Liao and Ai.)