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Your search keyword '"Yi, Jae-Seon"' showing total 31 results
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31 results on '"Yi, Jae-Seon"'

28. Antiinflammatory effect of the ethanol extract of Berberis koreana in a gerbil model of cerebral ischemia/reperfusion.

Author

Yoo, Ki-Yeon, Hwang, In Koo, Kim, Jong Dai, Kang, Il-Jun, Park, Jinseu, Yi, Jae-Seon, Kim, Jin-Kyu, Bae, Young-Soo, and Won, Moo-Ho

Abstract

Berberis koreana extract (BE) has a strong neuroprotective effect after ischemic stroke in gerbils, which is associated with the inhibition of the N-methyl-d-aspartate receptor. The present study examined the antiinflammatory mechanism of BE after ischemic damage in vitro and in vivo. The BE used contained on average 7.39 ± 0.78 mg/g of berberine. In PC12 cells with inflammation, prostaglandin E2 (PGE2) production was significantly reduced by BE. About 75% of pyramidal cells in the hippocampal CA1 region of gerbils exposed to 5 min of transient ischemia were protected from ischemic damage by BE. Cyclooxygenase-2 (COX-2) immunoreactivity and its protein level in the CA1 region of vehicle-treated animals exposed to an ischemic insult increased with time post-ischemia, whereas no such changes were observed in BE-treated animals exposed to ischemia. PGE2 production in BE-treated ischemic animals was significantly lower than that observed in vehicle-treated ischemic animals. Summarizing, the potent neuroprotective effect of BE was found to be due to the inhibitions of COX-2 expression and PGE2 production and its antiinflammatory activity. Copyright © 2008 John Wiley & Sons, Ltd. [ABSTRACT FROM AUTHOR]

Published
2008
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29. Induction of adventitious roots and analysis of ginsenoside content and the genes involved in triterpene biosynthesis in Panax ginseng.

Author

Han, Jung-Yeon, Jung, Su-Jin, Kim, Sang-Woo, Kwon, Yong-Soo, Yi, Myong-Jong, Yi, Jae-Seon, and Choi, Yong-Eui

Abstract

Adventitious roots were produced directly from root segments of Panax ginseng seedlings when cultured on an MS solid medium containing 3.0 mg L-1 IBA. Omitting NH4NO3 from this medium greatly enhanced both the frequency of adventitious root formation and the number of roots per expiants. This frequency declined markedly with the age of the root, but could be increased through repeated sub-culturing events. A two-step procedure that included NH4NO3 free media for the first two weeks of culture, followed by transfer onto media containing NH4NO3 for another four weeks, greatly improved total fresh weights of these adventitious roots compared with a method of continuous culture over six weeks in media that always contained NH4NO3. Expression of the genes involved in triterpene biosynthesis was analyzed by RT-PCR. Ginsenoside contents were enhanced by the omission of NH4NO3 and were also greatly increased by treatment with methyl jasmonate. [ABSTRACT FROM AUTHOR]

Published
2006
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30. Identification of Populations of Korean Mistletoe (Viscum AlbumL. Var. Coloratum) from Gangwon-Do in Korea by AFLP

Author

Yi, Jae-Seon, Kim, Chul-Woo, Lee, Ju-Kyong, and Meng, Fan-Juan

Abstract

ABSTRACTAmplified fragment length polymorphism (AFLP) was used to estimate genetic diversity among seven populations of Korean mistletoes (Viscum album L. var. coloratum) from Gangwon-Do in Korea. Out of the 64 primers, 5 produced steady, distinct and polymorphic fragments. Using 5 primers, 57 fragments were generated and 18 of these fragments (31.6%) were polymophic. The percentage of polymorphic fragments (PPB) for a single primer pair ranged from 14.3% to 57.1% with an average of 29.16%. In this study, the analysis showed that the average genetic diversity by Shannon's information index ranged from 0 to 1.00 within all populations. The genetic variation within populations (Havg/Ht) was 0.82, and the genetic variation between populations [(Ht—Havg)/Ht] was 0.18. The main factor responsible for this low level of differentiation between populations may be attributed to the limited geographic origin of the collection or the dispersal of plant material outside their original range, or high levels of gene flow between populations. Concerning the management of Korean mistletoes, the low genetic differentiation of populations indicates that we need to conserve more populations of Korean mistletoe.

Published
2013
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31. Micropropagation of eleutherococcus senticosus through axillary bud culture

Author

You, Xiang-Ling, Choi, Yong-Eui, and Yi, Jae-Seon

Abstract

Siberian ginseng (Eleutherococcus senticosus) is endangered mainly because of overexploitation. A multiplication system was established via in vitro nodal cutting of the stem. When cotyledonary seedling nodes were cultured on Murashige and Skoog (MS) medium with different concentrations of GA3 (1.0-30.0 mg/L) for 3 weeks, shoots with nodes were formed from the cotyledonary nodes. Thereafter, nodal segments (including an axillary bud) excised from these shoots were incubated on MS, Lloyd and McCown (WPM) and Schenk and Hildebrandt (SH) media with various concentrations of auxin, cytokinin and GA3. The most effective medium and hormonal combination for the shoot formation from axillary buds of nodal segments was MS medium containing BA (2.0 mg/L), IBA (0.5 mg/L) and GA3 (0.5 mg/L). In this environment, 99.93 % of axillary buds produced shoots, with an average shoot length of 16.95 mm and 2.75 nodes were formed per shoot. Rooting of axillary shoots was most effectively achieved on NH4NO3-free, 1/2 MS medium with 0.5 mg/L NAA, and the rooting rate was 58.5% after 30 days of culture. Plantlets with shoots and roots were transferred to a mixture of perlite and peatmoss (1:5, v/v), and the survival rate of plants grown on medium with either NAA or IBA after 3 months was 90.3% or 79.2%, respectively.

Published
2005
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