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In this study, leaf essential oil effects on glutamate toxicity model formed in cortex neurons and LN405 cell cultures were investigated. Antidiabetic activity was evaluated by α-amylase and α-glucosidase inhibitions. MIC was used for antimicrobial activity. Seven groups were examined with MTT. Glutamate 10-5 mM in cortex showed 62% viability whereas oil viability did not increase in a dose-dependent manner and the highest viability rate was observed. There are four types of glandular trichomes in leaf anatomy of walnut. The oil exhibited half as much α-glucosidase inhibitory activity with an IC50 value of 8105 μg/mL, compared to positive control acarbose (IC50 = 4762 μg/mL). MIC of oil was determined to be 625 μg/mL against E. coli, C. albicans, and C. parapsilosis, whereas S. aureus exhibited a MIC of 1250 μg/mL. Major compounds of oil were found as β-pinene (17.6%), α-pinene (11.3%), β-eudesmol (8.6%), and caryophyllene oxide (6.2%).

Amaç: Parkinson hastalığı, en sık görülen ikinci nörodejeneratif hastalıktır. Bu çalışmada, önemli biyoaktif bileşenlere sahip olduğu bilinen Capparis spinosa L. meyvelerinden elde edilen metanol ekstraktının in vitro deneysel Parkinson hastalığı modeline etkisinin araştırılması amaçlanmıştır.Gereç ve Yöntem: Alanya/Antalya’dan Capparis spinosa L. meyveleri toplandıktan sonra kurutulup öğütülerek metanol özütü hazırlandı. Flasklarda geliştirilen SH-SY5Y hücreleri, 96 oyuklu plakalara aktarıldı ve %80 hücre yoğunluğuna ulaşılana kadar inkübe edilmiştir. Parkinson hastalığı modeli oluşturulmadan 30 dakika önce hücrelere farklı dozlarda metanol özütü uygulanmıştır. Parkinson hastalığı modeli için SH-SY5Y hücreleri, 24 saat boyunca 200 uM 6-OHDA’ya maruz bırakılmıştır. 24 saatlik sürenin sonunda SH-SY5Y hücrelerinin canlılığını değerlendirmek için MTT analizi yapılmıştır. Hücre ortamındaki TOS, TAC ve IL-17A seviyeleri ELISA yöntemi kullanılarak belirlenmiştir. TNFα ve α-sinüklein ekspresyonu, immünohistokimyasal yöntem kullanılarak tespit edilmiştir.Bulgular: Hücre canlılığının tüm tedavi gruplarında 6-OHDA grubuna göre daha yüksek olduğu bulundu. 1500 µg/ml metanol özütü grubunda orta düzeyde TNFα ve a-sinüklein pozitifliği gözlendi. TOS ve TAC düzeylerinin doza bağlı olarak değiştiği belirlendi. Düşük dozlarda IL-17A seviyesinin düştüğü bulundu. Gruplar arasında istatistiksel anlamlılık saptandı.Sonuç: Bulgular incelendiğinde Capparis spinosa L. meyvelerinden elde edilen metanol ekstraktının düşük dozlarda oksidatif stres ve IL-17A düzeylerini azalttığı ve antioksidan kapasiteyi artırarak nöroprotektif etki sağladığı belirlendi., Aim: Parkinson’s disease (PD) is the second most widespread neurodegenerative disease. This study, it was aimed to investigate the effect of methanol extract obtained from Capparis spinosa L. fruits, which are known to have important bioactive components, on in-vitro experimental PD model.Material and Method: After collecting Capparis spinosa L. fruits from Alanya/Antalya, methanol extract was prepared by drying and grinding. SH-SY5Y cells grown in flasks were transferred to 96 well plates and were incubated until 80% cell density was reached. Different doses of methanol extract were applied to the cells 30 minutes before the PD model was formed. For the PD model, SH-SY5Y cells were exposed to 200 µM 6-OHDA for 24 hours. MTT analysis was performed to assess the viability of SH-SY5Y cells at the end of the 24-hour period. TOS, TAC, and IL-17A levels in the cell medium were determined using the ELISA method. Expression of TNFα and α-synuclein was defined using the immunohistochemical method.Results: Cell viability was found to be higher in all treatment groups than in the 6-OHDA group. Moderate levels of TNFα and α-synuclein positivity were observed in the 1500 µg/ml methanol extract group. It was determined that TOS and TAC levels change depending on the dose. It has been determined that the level of IL-17A decreases at low doses. Statistical significance was found between the groups.Conclusion: When the findings were examined, it was determined that the methanol extract obtained from Capparis spinosa L. fruits reduced oxidative stress and IL-17A levels at low doses and provided a neuroprotective effect by increasing the antioxidant capacity.

WOS: 000437492100018, PubMed: 30002894, In the title compound, C18H19NO3S, the morpholine ring adopts a chair conformation. The thiophene ring forms dihedral angles of 26.04 (9) and 74.07 (10)degrees with the benzene ring and the mean plane of the morpholine ring, respectively. The molecular conformation is stabilized by an O-H center dot center dot center dot N hydrogen bond. In the crystal, molecules are connected through C-H center dot center dot center dot O hydrogen bonds, forming wave-like layers parallel to the ab plane, which are further linked into a three-dimensional network by C-H center dot center dot center dot pi interactions involving the benzene rings and the methylene H atoms of the morpholine rings., Aksaray University, Science and Technology Application and Research Center, Aksaray, Turkey (State of Planning Organization) [2010K120480], The authors acknowledge the Aksaray University, Science and Technology Application and Research Center, Aksaray, Turkey, for the use of the Bruker SMART BREEZE CCD diffractometer (purchased under grant No. 2010K120480 of the State of Planning Organization)