130 results on '"Yeshitela, Biruk"'
Search Results
2. Incidence of typhoid fever in Burkina Faso, Democratic Republic of the Congo, Ethiopia, Ghana, Madagascar, and Nigeria (the Severe Typhoid in Africa programme): a population-based study
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Marks, Florian, Im, Justin, Park, Se Eun, Pak, Gi Deok, Jeon, Hyon Jin, Wandji Nana, Lady Rosny, Phoba, Marie-France, Mbuyi-Kalonji, Lisette, Mogeni, Ondari D, Yeshitela, Biruk, Panzner, Ursula, Cruz Espinoza, Ligia María, Beyene, Tigist, Owusu-Ansah, Michael, Twumasi-Ankrah, Sampson, Yeshambaw, Melese, Alemu, Ashenafi, Adewusi, Oluwafemi J, Adekanmbi, Olukemi, Higginson, Ellen, Adepoju, Akinlolu, Agbi, Sarah, Cakpo, Enoch G, Ogunleye, Veronica O, Tunda, Gaëlle Nkoji, Ikhimiukor, Odion O, Mbuyamba, Jules, Toy, Trevor, Agyapong, Francis Opoku, Osei, Isaac, Amuasi, John, Razafindrabe, Tsiriniaina Jean Luco, Raminosoa, Tiana Mirana, Nyirenda, Gabriel, Randriamampionona, Njariharinjakampionona, Seo, Hyeong Won, Seo, Hyejin, Siribie, Mohamadou, Carey, Megan E, Owusu, Michael, Meyer, Christian G, Rakotozandrindrainy, Ndrainaharimira, Sarpong, Nimarko, Razafindrakalia, Mathilde, Razafimanantsoa, Ravomialisoa, Ouedraogo, Moussa, Kim, Yeonseon J, Lee, Jooah, Zellweger, Raphaël M, Kang, Sophie S Y, Park, Ju Yeon, Crump, John A, Hardy, Liselotte, Jacobs, Jan, Garrett, Denise O, Andrews, Jason R, Poudyal, Nimesh, Kim, Deok Ryun, Clemens, John D, Baker, Stephen G, Kim, Jerome H, Dougan, Gordon, Sugimoto, Jonathan D, Van Puyvelde, Sandra, Kehinde, Aderemi, Popoola, Oluwafemi A, Mogasale, Vittal, Breiman, Robert F, MacWright, William R, Aseffa, Abraham, Tadesse, Birkneh Tilahun, Haselbeck, Andrea, Adu-Sarkodie, Yaw, Teferi, Mekonnen, Bassiahi, Abdramane Soura, Okeke, Iruka N, Lunguya-Metila, Octavie, Owusu-Dabo, Ellis, and Rakotozandrindrainy, Raphaël
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- 2024
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3. Magnitude and Molecular Characterization of Extended-Spectrum β-Lactamase Genes among Klebsiella pneumoniae Isolates in a Large Tertiary Hospital in Ethiopia
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Awoke, Tewachew, Teka, Brhanu, Aseffa, Abraham, Seman, Aminu, Sebre, Shemse, Yitayew, Berhanu, Yeshitela, Biruk, Abebe, Tamrat, Mihret, Adane, and Donelli, Gianfranco, editor
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- 2023
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4. Melioidosis in Africa: Time to uncover the true disease load
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Steinmetz, Ivo, Wagner, Gabriel E, Kanyala, Estelle, Sawadogo, Mamadou, Soumeya, Hema, Teferi, Mekonnen, Andargie, Emawayish, Yeshitela, Biruk, Atse-Achi, Louise Yaba, Sanogo, Moussa, Bonfoh, Bassirou, Rakotozandrindrainy, Raphael, Shongo, Celestin Pongombo, Pongombo, Mick Shongoya, Ilunga, Eric Kasamba, Lichtenegger, Sabine, Assig, Karoline, May, Jurgen, Bertherat, Eric, Owusu, Michael, Owusu-Dabo, Ellis, and Adu-Sarkodie, Yaw
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- 2018
5. The Magnitude of Carbapenemase and ESBL Producing Enterobacteriaceae Isolates from Patients with Urinary Tract Infections at Tikur Anbessa Specialized Teaching Hospital, Addis Ababa, Ethiopia
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Seman, Aminu, Sebre, Shemse, Awoke, Tewachew, Yeshitela, Biruk, Asseffa, Abraham, Asrat, Daniel, Abebe, Tamrat, Mihret, Adane, Crusio, Wim E., Series Editor, Dong, Haidong, Series Editor, Radeke, Heinfried H., Series Editor, Rezaei, Nima, Series Editor, Steinlein, Ortrud, Series Editor, Xiao, Junjie, Series Editor, and Donelli, Gianfranco, editor
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- 2022
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6. Occult hepatitis B virus infection among patients with chronic liver disease of unidentified cause, Addis Ababa Ethiopia
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Gissa, Selam Bogale, Minaye, Mengistu Erkie, Yeshitela, Biruk, Gemechu, Gizachew, Tesfaye, Abebech, Alemayehu, Dawit Hailu, Shewaye, Abel, Sultan, Amir, Mihret, Adane, and Mulu, Andargachew
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- 2022
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7. Detection of Salmonella Typhi nucleic acid by RT-PCR and anti-HlyE, -CdtB, -PilL, and -Vi IgM by ELISA at sites in Ghana, Madagascar and Ethiopia
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Panzner, Ursula, Mogeni, Ondari Daniel, Adu-Sarkodie, Yaw, Toy, Trevor, Jeon, Hyon Jin, Pak, Gi Deok, Park, Se Eun, Enuameh, Yeetey, Owusu-Dabo, Ellis, Van Tan, Trinh, Aseffa, Abraham, Teferi, Mekonnen, Yeshitela, Biruk, Baker, Stephen, Rakotozandrindrainy, Raphael, and Marks, Florian
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- 2022
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8. Magnitude and Molecular Characterization of Extended-Spectrum β-Lactamase Genes among Klebsiella pneumoniae Isolates in a Large Tertiary Hospital in Ethiopia
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Awoke, Tewachew, primary, Teka, Brhanu, additional, Aseffa, Abraham, additional, Seman, Aminu, additional, Sebre, Shemse, additional, Yitayew, Berhanu, additional, Yeshitela, Biruk, additional, Abebe, Tamrat, additional, and Mihret, Adane, additional
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- 2022
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9. Retrospective Analysis of Cholera/Acute Watery Diarrhea Outbreaks in Ethiopia From 2001 To 2023: Incidence, Case Fatality Rate, and Seasonal and Multiyear Epidemic Patterns.
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Demlie, Yeshambel Worku, Gedefaw, Abel, Jeon, Yeonji, Hailu, Dejene, Getahun, Tomas, Mogeni, Ondari D, Mukasa, David, Jang, Geun Hyeog, Pak, Gi Deok, Kim, Deok Ryun, Getachew, Edlawit Mesfin, Yeshitela, Biruk, Abebe, Samuyel Ayele, Edosa, Moti, Wossen, Mesfin, Teferi, Mekonnen, and Park, Se Eun
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PREVENTION of cholera ,DIARRHEA prevention ,DIARRHEA ,PEARSON correlation (Statistics) ,PUBLIC health surveillance ,COMMUNITY health services ,SEASONS ,HUMAN services programs ,DEATH ,RESEARCH funding ,CHOLERA ,LOGISTIC regression analysis ,MEDICAL case management ,RETROSPECTIVE studies ,DESCRIPTIVE statistics ,CHI-squared test ,PUBLIC health administration ,EPIDEMICS ,MEDICAL records ,ACQUISITION of data ,CONFIDENCE intervals ,MEDICAL needs assessment ,DEHYDRATION ,EMERGENCY management - Abstract
Background The Ethiopian government has developed the multisectoral cholera elimination plan (NCP) with an aim of reducing cholera incidence and case fatality rate (CFR). To better understand and monitor the progress of this plan, a comprehensive review of national cholera epidemiology is needed. Methods Reported data on cholera/acute watery diarrhea (AWD) cases in the past 20 years were extracted from the Ethiopian Public Health Institute and World Health Organization databases. Descriptive statistics, Pearson χ
2 , and logistic regression analyses were conducted. Results From January 2001 to November 2023, a total of 215 205 cholera/AWD cases, 2355 deaths with a cumulative CFR of 1.10% (95% confidence interval [CI], 1.092–1.095), and a mean annual incidence rate of 8.9/100 000 (95% CI, 6.5–11.3) were reported. Two major upsurges of cholera epidemics were found in the last two decades with mean attack rate (AR) of 20.57/100 000 in 2006–2010 and 14.83/100 000 in 2016–2020. Another resurgence of outbreaks occured in 2021–2023 (mean AR, 8.63/100 000). In 2015–2023, 54.0% (53 990/99 945) of cases were aged 15–44 years. National cholera CFR (3.13% [95% CI: 2.1–4.5]) was the highest in 2022. The 2015–2023 cumulative cholera CFR was different across regions: Benishangul Gumuz (6.07%), Gambela (1.89%), Sidama (1.42%), Southern Nation, Nationalities, and Peoples' (1.34%), Oromia (1.10%), and Amhara (1.09%). Cholera/AWD patients in older adults (≥45 years), severe dehydration, peak rainy season (June–August), and outpatients were associated with higher risk of death. Conclusions Cholera has been a public health problem in Ethiopia with case fatalities still above the global target. Case management needs to be improved particularly in outpatients and older populations. Outbreak preparedness should be rolled out well in advance of the typical rainy seasons. Significant investments are essential to advance the cholera surveillance system at healthcare setting and community level. Underlying factors of cholera deaths per areas should be further investigated to guide appropriate interventions to meet the NCP target by 2028. [ABSTRACT FROM AUTHOR]- Published
- 2024
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10. Coverage of Two-Dose Preemptive Cholera Mass Vaccination Campaign in High-Priority Hotspots in Shashemene, Oromia Region, Ethiopia.
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Park, Se Eun, Gedefaw, Abel, Hailu, Dejene, Jeon, Yeonji, Mogeni, Ondari D, Jang, Geun Hyeog, Mukasa, David, Mraidi, Ramzi, Kim, Deok Ryun, Getahun, Tomas, Getachew, Edlawit Mesfin, Yeshitela, Biruk, Abebe, Samuyel Ayele, Hussen, Mukemil, Demlie, Yeshambel Worku, and Teferi, Mekonnen
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PREVENTION of cholera ,MEDICAL protocols ,IMMUNIZATION ,DISEASE clusters ,PUBLIC health surveillance ,RESEARCH funding ,VACCINE effectiveness ,POPULATION geography ,DESCRIPTIVE statistics ,ORAL drug administration ,VACCINATION coverage ,CHOLERA vaccines ,SURVEYS ,CONFIDENCE intervals ,DRUG dosage ,DRUG administration - Abstract
Background Cholera is a public health priority in Ethiopia. The Ethiopian National Cholera Plan elaborates a multi-year scheme of oral cholera vaccine (OCV) use. Aligned with this, a preemptive OCV campaign was conducted under our Ethiopia Cholera Control and Prevention project. Here, we present the OCV vaccination outcomes. Method Cholera high-priority hotspots in the Oromia Region, Shashemene Town (ST) and Shashemene Woreda (SW), were selected. Four kebelles (Abosto, Alelu, Arada, and Awasho) in ST and 4 clusters (Faji Gole, Harabate, Toga, and Chabi) in SW were study sites with OCV areas nested within. A total of 40 000 and 60 000 people in ST and SW, respectively, were targeted for a 2-dose OCV (Euvichol-Plus) campaign in 11–15 May (first round [R1]) and 27–31 May (second round [R2]) 2022. Daily administrative OCV coverage and a coverage survey in 277 randomly selected households were conducted. Results The administrative OCV coverage was high: 102.0% for R1 and 100.5% for R2 in ST and 99.1% (R1) and 100.0% (R1) in SW. The coverage survey showed 78.0% (95% confidence interval [CI]: 73.1–82.9) of household members with 2-dose OCV and 16.8% (95% CI: 12.4–21.3) with no OCV in ST; and 83.1% (95% CI: 79.6–86.5) with 2-dose OCV and 11.8% (95% CI: 8.8–14.8) with no OCV in SW. The 2-dose coverages in 1–4-, 5–14-, and ≥15-year age groups were 88.3% (95% CI: 70.6–96.1), 88.9% (95% CI: 82.1–95.7), and 71.3% (95% CI: 64.2–78.3), respectively, in ST and 78.2% (95% CI: 68.8–87.7), 91.0% (95% CI: 86.6–95.3), and 78.7% (95% CI: 73.2–84.1) in SW. Conclusions High 2-dose OCV coverage was achieved. Cholera surveillance is needed to assess the vaccine impact and effectiveness. [ABSTRACT FROM AUTHOR]
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- 2024
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11. Healthcare Seeking Behavior and Disease Perception Toward Cholera and Acute Diarrhea Among Populations Living in Cholera High-Priority Hotspots in Shashemene, Ethiopia.
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Getahun, Tomas, Hailu, Dejene, Mogeni, Ondari D, Getachew, Edlawit Mesfin, Yeshitela, Biruk, Jeon, Yeonji, Gedefaw, Abel, Abebe, Samuyel Ayele, Hundito, Ermiyas, Mukasa, David, Jang, Geun Hyeog, Pak, Gi Deok, Kim, Deok Ryun, Demlie, Yeshambel Worku, Hussen, Mukemil, Teferi, Mekonnen, and Park, Se Eun
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DIARRHEA prevention ,PREVENTION of cholera ,DISEASE clusters ,CROSS-sectional method ,HEALTH services accessibility ,ATTITUDES toward illness ,HEALTH attitudes ,RESEARCH funding ,STATISTICAL sampling ,COST analysis ,HELP-seeking behavior ,DESCRIPTIVE statistics ,SURVEYS ,MEDICAL needs assessment ,DATA analysis software ,COMPARATIVE studies - Abstract
Background Healthcare seeking behavior (HSB) and community perception on cholera can influence its management. We conducted a cross-sectional survey to generate evidence on cholera associated HSB and disease perception in populations living in cholera hotspots in Ethiopia. Methods A total of 870 randomly selected households (HHs) in Shashemene Town (ST) and Shashemene Woreda (SW) participated in our survey in January 2022. Results Predominant HHs (91.0%; 792/870) responded "primary health center" as the nearest healthcare facility (HCF). Around 57.4% (247/430) of ST HHs traveled <30 minutes to the nearest HCF. In SW, 60.2% (265/440) of HHs travelled over 30 minutes and 25.9% (114/440) over 4 km. Two-thirds of all HHs paid
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- 2024
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12. Dissecting Water, Sanitation, and Hygiene (WaSH) to Assess Risk Factors for Cholera in Shashemene, Oromia Region, Ethiopia.
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Hailu, Dejene, Jeon, Yeonji, Gedefaw, Abel, Kim, Jong-Hoon, Mraidi, Ramzi, Getahun, Tomas, Mogeni, Ondari D, Getachew, Edlawit Mesfin, Jang, Geun Hyeog, Mukasa, David, Pak, Gi Deok, Kim, Deok Ryun, Abebe, Samuyel Ayele, Yeshitela, Biruk, Edosa, Moti, Demlie, Yeshambel Worku, Park, Se Eun, and Teferi, Mekonnen
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SANITATION ,CROSS-sectional method ,RESEARCH funding ,CHOLERA ,SOCIOECONOMIC factors ,RESIDENTIAL patterns ,HYGIENE ,RETROSPECTIVE studies ,DESCRIPTIVE statistics ,WATER supply ,SOCIAL status ,ODDS ratio ,MEDICAL records ,ACQUISITION of data ,WATER pollution ,METROPOLITAN areas ,SOCIODEMOGRAPHIC factors ,CONFIDENCE intervals ,EDUCATIONAL attainment ,DISEASE risk factors - Abstract
Background Cholera outbreaks have afflicted Ethiopia, with nearly 100 000 cases and 1030 deaths reported from 2015 to 2023, emphasizing the critical need to understand water, sanitation, and hygiene (WaSH) risk factors. Methods We conducted a cross-sectional household (HH) survey among 870 HHs in Shashemene Town and Shashemene Woreda, alongside extracting retrospective cholera case data from the Ethiopian Public Health Institute database. Relationships between WaSH and sociodemographic/economic-levels of HHs were examined. WaSH status and cholera attack rates (ARs) were described at kebele-level using geospatial mapping, and their association was statistically analyzed. Results Access to basic drinking water, sanitation, and hygiene facilities was limited, with 67.5% (95% confidence interval, 64.4–70.6), 73.4% (70.3–76.3), and 30.3% (27.3–33.3) of HHs having access, respectively. Better WaSH practices were associated with urban residence (adjusted odds ratio, 1.7, [95% confidence interval, 1.1–2.7]), higher educational levels (2.7 [1.2–5.8]), and wealth (2.5 [1.6–4.0]). The association between cholera ARs and at least basic WaSH status was not statistically significant (multiple R
2 = 0.13; P =.36), although localized effects were suggested for sanitation (Moran I = 0.22; P =.024). Conclusions Addressing gaps in WaSH access and hygiene practices is crucial for reducing cholera risk. Further analyses with meaningful covariates and increased sample sizes are necessary to understand the association between cholera AR and specific WaSH components. [ABSTRACT FROM AUTHOR]- Published
- 2024
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13. Comprehensive Review on the Use of Oral Cholera Vaccine (OCV) in Ethiopia: 2019 to 2023.
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Edosa, Moti, Jeon, Yeonji, Gedefaw, Abel, Hailu, Dejene, Getachew, Edlawit Mesfin, Mogeni, Ondari D, Jang, Geun Hyeog, Mukasa, David, Yeshitela, Biruk, Getahun, Tomas, Lynch, Julia, Bouhenia, Malika, Demlie, Yeshambel Worku, Hussen, Mukemil, Wossen, Mesfin, Teferi, Mekonnen, and Park, Se Eun
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PREVENTION of cholera ,PREVENTION of epidemics ,MEDICAL protocols ,IMMUNIZATION ,PUBLIC hospitals ,RESEARCH funding ,SEX distribution ,ORAL drug administration ,RETROSPECTIVE studies ,DESCRIPTIVE statistics ,POPULATION geography ,AGE distribution ,CHOLERA vaccines ,VACCINATION coverage ,SURVEYS ,MEDICAL records ,ACQUISITION of data ,DRUG dosage ,DRUG administration - Abstract
Background Cholera outbreaks in Ethiopia necessitate frequent mass oral cholera vaccine (OCV) campaigns. Despite this, there is a notable absence of a comprehensive summary of these campaigns. Understanding national OCV vaccination history is essential to design appropriate and effective cholera control strategies. Here, we aimed to retrospectively review all OCV vaccination campaigns conducted across Ethiopia between 2019 and 2023. Methods The OCV request records from 2019 to October 2023 and vaccination campaign reports for the period from 2019 to December 2023 were retrospectively accessed from the Ethiopia Public Health Institute (EPHI) database. Descriptive analysis was conducted using the retrospective data collected. Results From 2019 to October 2023, Ethiopian government requested 32 044 576 OCV doses (31 899 576 doses to global stockpile; 145 000 doses to outside of stockpile). Around 66.3% of requested doses were approved; of which 90.4% were received. Fifteen OCV campaigns (12 reactive and 3 pre-emptive) were conducted, including five two-dose campaigns with varying dose intervals and single-dose campaigns partially in 2019 and entirely in 2021, 2022 and 2023. Overall vaccine administrative coverage was high; except for Tigray region (41.8% in the 1st round; 2nd round didn't occur). The vaccine administrative coverage records were documented, but no OCV coverage survey data was available. Conclusions This study represents the first comprehensive review of OCV campaigns in Ethiopia spanning the last five years. Its findings offer valuable insights into informing future cholera control strategies, underscoring the importance of monitoring and evaluation despite resource constraints. Addressing the limitations in coverage survey data availability is crucial for enhancing the efficacy of future campaigns. [ABSTRACT FROM AUTHOR]
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- 2024
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14. The Magnitude of Carbapenemase and ESBL Producing Enterobacteriaceae Isolates from Patients with Urinary Tract Infections at Tikur Anbessa Specialized Teaching Hospital, Addis Ababa, Ethiopia
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Seman, Aminu, primary, Sebre, Shemse, additional, Awoke, Tewachew, additional, Yeshitela, Biruk, additional, Asseffa, Abraham, additional, Asrat, Daniel, additional, Abebe, Tamrat, additional, and Mihret, Adane, additional
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- 2021
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15. Characterization of Typhoid Intestinal Perforation in Africa: Results From the Severe Typhoid Fever Surveillance in Africa Program
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Birkhold, Megan, primary, Datta, Shrimati, additional, Pak, Gi Deok, additional, Im, Justin, additional, Ogundoyin, Olakayode O, additional, Olulana, Dare I, additional, Lawal, Taiwo A, additional, Afuwape, Oludolapo O, additional, Kehinde, Aderemi, additional, Phoba, Marie-France, additional, Nkoji, Gaëlle, additional, Aseffa, Abraham, additional, Teferi, Mekonnen, additional, Yeshitela, Biruk, additional, Popoola, Oluwafemi, additional, Owusu, Michael, additional, Nana, Lady Rosny Wandji, additional, Cakpo, Enoch G, additional, Ouedraogo, Moussa, additional, Ouangre, Edgar, additional, Ouedraogo, Isso, additional, Heroes, Anne-Sophie, additional, Jacobs, Jan, additional, Mogeni, Ondari D, additional, Haselbeck, Andrea, additional, Sukri, Leah, additional, Neuzil, Kathleen M, additional, Metila, Octavie Lunguya, additional, Owusu-Dabo, Ellis, additional, Adu-Sarkodie, Yaw, additional, Bassiahi, Abdramane Soura, additional, Rakotozandrindrainy, Raphaël, additional, Okeke, Iruka N, additional, Zellweger, Raphaël M, additional, and Marks, Florian, additional
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- 2023
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16. Incidence of invasive salmonella disease in sub-Saharan Africa: a multicentre population-based surveillance study
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Marks, Florian, von Kalckreuth, Vera, Aaby, Peter, Adu-Sarkodie, Yaw, El Tayeb, Muna Ahmed, Ali, Mohammad, Aseffa, Abraham, Baker, Stephen, Biggs, Holly M, Bjerregaard-Andersen, Morten, Breiman, Robert F, Campbell, James I, Cosmas, Leonard, Crump, John A, Espinoza, Ligia Maria Cruz, Deerin, Jessica Fung, Dekker, Denise Myriam, Fields, Barry S, Gasmelseed, Nagla, Hertz, Julian T, Van Minh Hoang, Nguyen, Im, Justin, Jaeger, Anna, Jeon, Hyon Jin, Kabore, Leon Parfait, Keddy, Karen H, Konings, Frank, Krumkamp, Ralf, Ley, Benedikt, Løfberg, Sandra Valborg, May, Jürgen, Meyer, Christian G, Mintz, Eric D, Montgomery, Joel M, Niang, Aissatou Ahmet, Nichols, Chelsea, Olack, Beatrice, Pak, Gi Deok, Panzner, Ursula, Park, Jin Kyung, Park, Se Eun, Rabezanahary, Henintsoa, Rakotozandrindrainy, Raphaël, Raminosoa, Tiana Mirana, Razafindrabe, Tsiriniaina Jean Luco, Sampo, Emmanuel, Schütt-Gerowitt, Heidi, Sow, Amy Gassama, Sarpong, Nimako, Seo, Hye Jin, Sooka, Arvinda, Soura, Abdramane Bassiahi, Tall, Adama, Teferi, Mekonnen, Thriemer, Kamala, Warren, Michelle R, Yeshitela, Biruk, Clemens, John D, and Wierzba, Thomas F
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- 2017
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17. Characterization of Typhoid Intestinal Perforation in Africa: Results From the Severe Typhoid Fever Surveillance in Africa Program
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Birkhold, Megan, Datta, Shrimati, Pak, Gi Deok, Im, Justin, Ogundoyin, Olakayode O, Olulana, Dare I, Lawal, Taiwo A, Afuwape, Oludolapo O, Kehinde, Aderemi, Phoba, Marie-France, Nkoji, Gaëlle, Aseffa, Abraham, Teferi, Mekonnen, Yeshitela, Biruk, Popoola, Oluwafemi, Owusu, Michael, Nana, Lady Rosny Wandji, Cakpo, Enoch G, Ouedraogo, Moussa, Ouangre, Edgar, Ouedraogo, Isso, Heroes, Anne-Sophie, Jacobs, Jan, Mogeni, Ondari D, Haselbeck, Andrea, Sukri, Leah, Neuzil, Kathleen M, Metila, Octavie Lunguya, Owusu-Dabo, Ellis, Adu-Sarkodie, Yaw, Bassiahi, Abdramane Soura, Rakotozandrindrainy, Raphaël, Okeke, Iruka N, Zellweger, Raphaël M, Marks, Florian, Birkhold, Megan [0000-0002-5746-1321], Im, Justin [0000-0002-3762-084X], Okeke, Iruka N [0000-0002-1694-7587], Marks, Florian [0000-0002-6043-7170], and Apollo - University of Cambridge Repository
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intestinal perforation ,Africa ,Salmonella Typhi ,severe typhoid ,typhoid fever - Abstract
BACKGROUND: Typhoid intestinal perforation (TIP) remains the most serious complication of typhoid fever. In many countries, the diagnosis of TIP relies on intraoperative identification, as blood culture and pathology capacity remain limited. As a result, many cases of TIP may not be reported as typhoid. This study demonstrates the burden of TIP in sites in Burkina Faso, Democratic Republic of Congo (DRC), Ethiopia, Ghana, Madagascar, and Nigeria. METHODS: Patients with clinical suspicion of nontraumatic intestinal perforation were enrolled and demographic details, clinical findings, surgical records, blood cultures, tissue biopsies, and peritoneal fluid were collected. Participants were then classified as having confirmed TIP, probable TIP, possible TIP, or clinical intestinal perforation based on surgical descriptions and cultures. RESULTS: A total of 608 participants were investigated for nontraumatic intestinal perforation; 214 (35%) participants had surgically-confirmed TIP and 33 participants (5%) had culture-confirmed typhoid. The overall proportion of blood or surgical site Salmonella enterica subspecies enterica serovar Typhi positivity in surgically verified TIP cases was 10.3%. TIP was high in children aged 5-14 years in DRC, Ghana, and Nigeria. We provide evidence for correlation between monthly case counts of S. Typhi and the occurrence of intestinal perforation. CONCLUSIONS: Low S. Typhi culture positivity rates, as well as a lack of blood and tissue culture capability in many regions where typhoid remains endemic, significantly underestimate the true burden of typhoid fever. The occurrence of TIP may indicate underlying typhoid burden, particularly in countries with limited culture capability.
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- 2023
18. The Relationship Between Invasive Nontyphoidal Salmonella Disease, Other Bacterial Bloodstream Infections, and Malaria in Sub-Saharan Africa
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Park, Se Eun, Pak, Gi Deok, Aaby, Peter, Adu-Sarkodie, Yaw, Ali, Mohammad, Aseffa, Abraham, Biggs, Holly M., Bjerregaard-Andersen, Morten, Breiman, Robert F., Crump, John A., Espinoza, Ligia Maria Cruz, Eltayeb, Muna Ahmed, Gasmelseed, Nagla, Hertz, Julian T., Im, Justin, Jaeger, Anna, Kabore, Leon Parfait, von Kalckreuth, Vera, Keddy, Karen H., Konings, Frank, Krumkamp, Ralf, MacLennan, Calman A., Meyer, Christian G., Montgomery, Joel M., Niang, Aissatou Ahmet, Nichols, Chelsea, Olack, Beatrice, Panzner, Ursula, Park, Jin Kyung, Rabezanahary, Henintsoa, Rakotozandrindrainy, Raphaël, Sampo, Emmanuel, Schütt-Gerowitt, Heidi, Sooka, Arvinda, Soura, Abdramane Bassiahi, Sow, Amy Gassama, Tall, Adama, Teferi, Mekonnen, Yeshitela, Biruk, May, Jürgen, Wierzba, Thomas F., Clemens, John D., Baker, Stephen, and Marks, Florian
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- 2016
19. Utilization of Healthcare in the Typhoid Fever Surveillance in Africa Program
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Panzner, Ursula, Pak, Gi Deok, Aaby, Peter, Adu-Sarkodie, Yaw, Ali, Mohammad, Aseffa, Abraham, Baker, Stephen, Bjerregaard-Andersen, Morten, Crump, John A., Deerin, Jessica, Espinoza, Ligia Maria Cruz, Gasmelseed, Nagla, Heriniaina, Jean Noël, Hertz, Julian T., Im, Justin, von Kalckreuth, Vera, Keddy, Karen H., Lankoande, Bruno, Løfberg, Sandra, Meyer, Christian G., Oresto, Michael Munishi, Park, Jin Kyung, Park, Se Eun, Rakotozandrindrainy, Raphaël, Sarpong, Nimako, Soura, Abdramane Bassiahi, Sow, Amy Gassama, Tall, Adama, Teferi, Mekonnen, Worku, Alemayehu, Yeshitela, Biruk, Wierzba, Thomas F., and Marks, Florian
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- 2016
20. Bloodstream Infections and Frequency of Pretreatment Associated With Age and Hospitalization Status in Sub-Saharan Africa
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Nichols, Chelsea, Espinoza, Ligia Maria Cruz, von Kalckreuth, Vera, Aaby, Peter, El Tayeb, Muna Ahmed, Ali, Mohammad, Aseffa, Abraham, Bjerregaard-Andersen, Morten, Breiman, Robert F., Cosmas, Leonard, Crump, John A., Dekker, Denise Myriam, Sow, Amy Gassama, Gasmelseed, Nagla, Hertz, Julian T., Im, Justin, Kabore, Leon Parfait, Keddy, Karen H., Konings, Frank, Løfberg, Sandra Valborg, Meyer, Christian G., Montgomery, Joel M., Niang, Aissatou, Njariharinjakamampionona, Andriamampionona, Olack, Beatrice, Pak, Gi Deok, Panzner, Ursula, Park, Jin Kyung, Park, Se Eun, Rabezanahary, Henintsoa, Rakotondrainiarivelo, Jean Philibert, Rakotozandrindrainy, Raphaël, Raminosoa, Tiana Mirana, Rubach, Matthew P., Teferi, Mekonnen, Seo, Hye Jin, Sooka, Arvinda, Soura, Abdramane, Tall, Adama, Toy, Trevor, Yeshitela, Biruk, Clemens, John D., Wierzba, Thomas F., Baker, Stephen, and Marks, Florian
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- 2015
21. Correction to: Prevalence and phenotypic characterization of Enterococcus species isolated from clinical samples of pediatric patients in Jimma University Specialized Hospital, south west Ethiopia
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Toru, Milkiyas, Beyene, Getnet, Kassa, Tesfaye, Gizachew, Zeleke, Howe, Rawleigh, and Yeshitela, Biruk
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- 2019
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22. The phylogeography and incidence of multi-drug resistant typhoid fever in sub-Saharan Africa
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Park, Se Eun, Pham, Duy Thanh, Boinett, Christine, Wong, Vanessa K., Pak, Gi Deok, Panzner, Ursula, Espinoza, Ligia Maria Cruz, von Kalckreuth, Vera, Im, Justin, Schütt-Gerowitt, Heidi, Crump, John A., Breiman, Robert F., Adu-Sarkodie, Yaw, Owusu-Dabo, Ellis, Rakotozandrindrainy, Raphaël, Soura, Abdramane Bassiahi, Aseffa, Abraham, Gasmelseed, Nagla, Keddy, Karen H., May, Jürgen, Sow, Amy Gassama, Aaby, Peter, Biggs, Holly M., Hertz, Julian T., Montgomery, Joel M., Cosmas, Leonard, Olack, Beatrice, Fields, Barry, Sarpong, Nimako, Razafindrabe, Tsiriniaina Jean Luco, Raminosoa, Tiana Mirana, Kabore, Leon Parfait, Sampo, Emmanuel, Teferi, Mekonnen, Yeshitela, Biruk, El Tayeb, Muna Ahmed, Sooka, Arvinda, Meyer, Christian G., Krumkamp, Ralf, Dekker, Denise Myriam, Jaeger, Anna, Poppert, Sven, Tall, Adama, Niang, Aissatou, Bjerregaard-Andersen, Morten, Løfberg, Sandra Valborg, Seo, Hye Jin, Jeon, Hyon Jin, Deerin, Jessica Fung, Park, Jinkyung, Konings, Frank, Ali, Mohammad, Clemens, John D., Hughes, Peter, Sendagala, Juliet Nsimire, Vudriko, Tobias, Downing, Robert, Ikumapayi, Usman N., Mackenzie, Grant A., Obaro, Stephen, Argimon, Silvia, Aanensen, David M., Page, Andrew, Keane, Jacqueline A., Duchene, Sebastian, Dyson, Zoe, Holt, Kathryn E., Dougan, Gordon, Marks, Florian, and Baker, Stephen
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- 2018
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23. Occurrences of self-administered antibiotics, culture diagnostic challenges and multi-drug resistant S. aureus among Ethiopian pyogenic/bacterial meningitis patients: 2020-2021
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Mihret, Wude, primary, Alemu, Ashenafi, additional, Hussein, Ekram, additional, Merid, Yared, additional, Asres, Mezgebu Silamsaw, additional, Ayele, Samuel, additional, Mola, Kasse, additional, Yeshitela, Biruk, additional, Beyene, Getachew Tesfaye, additional, Mekuria, Biruktawit Amdemariam, additional, Yimer, Marechign, additional, and Mihret, Adane, additional
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- 2022
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24. Infectious Disease Control and Management in Ethiopia: A Case Study of Cholera
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Park, Se Eun, primary, Jeon, Yeonji, additional, Kang, Sunjoo, additional, Gedefaw, Abel, additional, Hailu, Dejene, additional, Yeshitela, Biruk, additional, Edosa, Moti, additional, Getaneh, Mesfin Wossen, additional, and Teferi, Mekonnen, additional
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- 2022
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25. Prevalence and Molecular Characterization of Extended Spectrum β-Lactamase and Carbapenemase-Producing Enterobacteriaceae Isolates from Bloodstream Infection Suspected Patients in Addis Ababa, Ethiopia
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Seman,Aminu, Mihret,Adane, Sebre,Shemse, Awoke,Tewachew, Yeshitela,Biruk, Yitayew,Berhanu, Aseffa,Abraham, Asrat,Daniel, Abebe,Tamrat, Seman,Aminu, Mihret,Adane, Sebre,Shemse, Awoke,Tewachew, Yeshitela,Biruk, Yitayew,Berhanu, Aseffa,Abraham, Asrat,Daniel, and Abebe,Tamrat
- Abstract
Aminu Seman,1,2 Adane Mihret,1,2 Shemse Sebre,1,2 Tewachew Awoke,3 Biruk Yeshitela,2 Berhanu Yitayew,4 Abraham Aseffa,2 Daniel Asrat,1 Tamrat Abebe1 1Department of Microbiology, Immunology, and Parasitology, School of Medicine, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia; 2Bacterial and Viral Disease Research Directorate, Armauer Hansen Research Institute, Addis Ababa, Ethiopia; 3Department of Medical Laboratory Sciences, College of Medicine and Health Sciences, Bahir Dar University, Bahir Dar, Ethiopia; 4Department of Medical Laboratory Science, College of Medicine and Health Sciences, Debre Berhan University, Debre Berhan, EthiopiaCorrespondence: Aminu Seman, Department of Microbiology, Immunology, and Parasitology, School of Medicine, College of Health Sciences, Addis Ababa University, P.O. Box: 9086, Addis Ababa, Ethiopia, Tel +251 920 747 176, Email aminu.seman@aau.edu.et; aminumifta54@gmail.comBackground: Production of Extended spectrum beta-lactamase (ESBL) and Carbapenemase is the most common strategy for drug resistance in clinical isolates of Enterobacteriaceae. This study was conducted to determine the magnitude of ESBL and Carbapenemase production (CPE) among clinical isolates of Enterobacteriaceae causing bloodstream infections (BSI) in Ethiopia.Methods: A cross-sectional study was performed from September 2018 to January 2019 in Ethiopia. A total of 2397 BSI suspected patients were enrolled and blood culture was performed using a BacT/Alert instrument in combination with conventional methods for identification. After antimicrobial susceptibility test, phenotypic confirmation of ESBLs was done by combined disc-diffusion. Meanwhile carbapenemase production was done by modified carbapenem inactivation method. Multiplex PCR was conducted to detect the presence of blaCTX-M,blaSHV, blaTEM, blaKPC and blaNDM genes.Results: A total of 104 (4.3%) Enterobacteriaceae were isolated from 2397 BSI suspected patients. Klebsiella pneumoni
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- 2022
26. Detection of blaKPC and blaNDM carbapenemase genes among Klebsiella pneumoniae isolates in Addis Ababa, Ethiopia: Dominance of blaNDM
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Awoke, Tewachew, primary, Teka, Brhanu, additional, Aseffa, Abraham, additional, Sebre, Shemse, additional, Seman, Aminu, additional, Yeshitela, Biruk, additional, Abebe, Tamrat, additional, and Mihret, Adane, additional
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- 2022
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27. Prevalence and Molecular Characterization of Extended Spectrum β-Lactamase and Carbapenemase-Producing Enterobacteriaceae Isolates from Bloodstream Infection Suspected Patients in Addis Ababa, Ethiopia
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Seman, Aminu, primary, Mihret, Adane, additional, Sebre, Shemse, additional, Awoke, Tewachew, additional, Yeshitela, Biruk, additional, Yitayew, Berhanu, additional, Aseffa, Abraham, additional, Asrat, Daniel, additional, and Abebe, Tamrat, additional
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- 2022
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28. Additional file 1 of Detection of Salmonella Typhi nucleic acid by RT-PCR and anti-HlyE, -CdtB, -PilL, and -Vi IgM by ELISA at sites in Ghana, Madagascar and Ethiopia
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Panzner, Ursula, Mogeni, Ondari Daniel, Adu-Sarkodie, Yaw, Toy, Trevor, Jeon, Hyon Jin, Pak, Gi Deok, Park, Se Eun, Enuameh, Yeetey, Owusu-Dabo, Ellis, Van Tan, Trinh, Aseffa, Abraham, Teferi, Mekonnen, Yeshitela, Biruk, Baker, Stephen, Rakotozandrindrainy, Raphael, and Marks, Florian
- Abstract
Additional file 1. Location of sites in Ethiopia [1A], Ghana [1B] and Madagascar [1C]. Notes: The location of each site is indicated as a black dot and the site’s name in red font; Ethiopia (Figure 1A): nine regional states (black italic, capital letters) and two chartered cities (black italic, lower letters) are shown;SNNPR=Southern Nations, Nationalities and People’s Region; Ghana (Figure 1B): ten regions (black italic, capital letters) are shown; Madagascar (Figure 1C): six provinces (black italic, capital letters) are shown.
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- 2022
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29. Additional file 2 of Detection of Salmonella Typhi nucleic acid by RT-PCR and anti-HlyE, -CdtB, -PilL, and -Vi IgM by ELISA at sites in Ghana, Madagascar and Ethiopia
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Panzner, Ursula, Mogeni, Ondari Daniel, Adu-Sarkodie, Yaw, Toy, Trevor, Jeon, Hyon Jin, Pak, Gi Deok, Park, Se Eun, Enuameh, Yeetey, Owusu-Dabo, Ellis, Van Tan, Trinh, Aseffa, Abraham, Teferi, Mekonnen, Yeshitela, Biruk, Baker, Stephen, Rakotozandrindrainy, Raphael, and Marks, Florian
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Additional file 2. Distribution of ELISA OD values by antigen.
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- 2022
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30. Additional file 3 of Detection of Salmonella Typhi nucleic acid by RT-PCR and anti-HlyE, -CdtB, -PilL, and -Vi IgM by ELISA at sites in Ghana, Madagascar and Ethiopia
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Panzner, Ursula, Mogeni, Ondari Daniel, Adu-Sarkodie, Yaw, Toy, Trevor, Jeon, Hyon Jin, Pak, Gi Deok, Park, Se Eun, Enuameh, Yeetey, Owusu-Dabo, Ellis, Van Tan, Trinh, Aseffa, Abraham, Teferi, Mekonnen, Yeshitela, Biruk, Baker, Stephen, Rakotozandrindrainy, Raphael, and Marks, Florian
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Additional file 3. Mean ELISA ODs by antigen. Notes: OD=optical density; red dot=mean of original data; blue dot=mean of each 1,000 bootstrap random samples; light red dashed line=upper and lower 95% confidence interval (CI) of original data; dark red dashed line=upper and lower 99% confidence interval (CI) of original data
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- 2022
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31. The Severe Typhoid in Africa Program: Incidences Of Typhoid Fever in Burkina Faso, Democratic Republic of Congo, Ethiopia, Ghana, Madagascar, And Nigeria
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Marks, Florian, primary, Im, Justin, additional, Park, Se Eun, additional, Pak, Gideok, additional, Jeon, Hyonjin, additional, Wandji Nana, Lady Rosny, additional, Phoba, Marie-France, additional, Mbuyi-Kalonji, Lisette, additional, Mogeni, Ondari D., additional, Yeshitela, Biruk, additional, Panzner, Ursula, additional, Cruz Espinoza, Ligia Maria, additional, Beyene, Tigist, additional, Owusu Ansah, Michael, additional, Twumasi-Ankrah, Sampson, additional, Yeshambaw, Melese, additional, Alemu, Ashenafi, additional, Adewusi, Oluwafemi, additional, Adekanmbi, Olukemi, additional, Higginson, Ellen, additional, Adepoju, Akinlolu, additional, Agbi, Sarah, additional, Cakpo, Enoch G., additional, Ogunleye, Veronica O., additional, Nkoji, Gaëlle, additional, Ikhimiukor, Odion O., additional, Mbuyamba, Jules, additional, Toy, Trevor, additional, Opoku Agyapong, Francis, additional, Razafindrabe, Tsiriniaina Jean Luco, additional, Raminosoa, Tiana Mirana, additional, Nyrienda, Gabriel, additional, Seo, Hyeong Won, additional, Seo, Hyejin, additional, Siribie, Mohamadou, additional, Carey, Megan E., additional, Owusu, Michael, additional, Meyer, Christian G., additional, Rakotozandrindrainy, Ndrainaharimira, additional, Sarpong, Nimarko, additional, Razafindrakalia, Mathilde, additional, Razafimanantsoa, Ravomialisoa, additional, Ouedraogo, Moussa, additional, Kim, Yeonseon J., additional, Zellweger, Raphaël M., additional, Kang, Sophie SY, additional, Park, Juyeon, additional, Crump, John A., additional, Hardy, Liselotte, additional, Jacobs, Jan, additional, Garrett, Denise, additional, Andrews, Jason, additional, Poudyal, Nimesh, additional, Kim, Deok Ryun, additional, Clemens, John D., additional, Baker, Stephen G., additional, Kim, Jerome H., additional, Dougan, Gordon, additional, Sugimoto, Jonathan D., additional, Van Puyvelde, Sandra, additional, Kehinde, Aderemi, additional, Popoola, Oluwafemi A., additional, Mogasale, Vittal, additional, Breiman, Robert, additional, MacWright, William, additional, Aseffa, Abraham, additional, Tadesse, Birkneh Tilahun, additional, Haselbeck, Andrea, additional, Adu-Sarkodie, Yaw, additional, Teferi, Mekonnen, additional, Soura Bassiahi, Abdramane, additional, Okeke, Iruka, additional, Lunguya-Metila, Octavie, additional, Owusu-Dabo, Ellis, additional, and Rakotozandrindrainy, Raphaël, additional
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- 2022
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32. Detection of Salmonella Typhi nucleic acid by RT-PCR and anti-HlyE, -CdtB, -PilL, and -Vi IgM by ELISA at sites in Ghana, Madagascar and Ethiopia
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Panzner, Ursula, Mogeni, Ondari Daniel, Adu-Sarkodie, Yaw, Toy, Trevor, Jeon, Hyon Jin, Pak, Gi Deok, Park, Se Eun, Enuameh, Yeetey, Owusu-Dabo, Ellis, Van Tan, Trinh, Aseffa, Abraham, Teferi, Mekonnen, Yeshitela, Biruk, Baker, Stephen, Rakotozandrindrainy, Raphael, Marks, Florian, and Apollo - University of Cambridge Repository
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Corneal Dystrophies, Hereditary ,Male ,HlyE-/CdtB-/PilL-/Vi-antigen ,Surveillance ,Adolescent ,Fever ,Reverse Transcriptase Polymerase Chain Reaction ,Enzyme-Linked Immunosorbent Assay ,Febrile illness ,Salmonella typhi ,Ghana ,Infectious Diseases ,Immunoglobulin M ,Salmonella ,Nucleic Acids ,Madagascar ,Humans ,ELISA ,Female ,Ethiopia ,Typhoid Fever - Abstract
Background We aimed to assess the prevalence of Salmonella Typhi through DNA and IgM-antibody detection methods as a prelude to extended surveillance activities at sites in Ghana, Madagascar, and Ethiopia. Methods We performed species-specific real-time polymerase reaction (RT-PCR) to identify bacterial nucleic acid, and enzyme-linked immunosorbent assay (ELISA) for detecting HlyE/STY1498-, CdtB/STY1886-, pilL/STY4539- and Vi-antigens in blood and biopsy specimens of febrile and non-febrile subjects. We generated antigen-specific ELISA proxy cut-offs by change-point analyses, and utilized cumulative sum as detection method coupled with 1000 repetitive bootstrap analyses. We computed prevalence rates in addition to odds ratios to assess correlations between ELISA outcomes and participant characteristics. Results Definitive positive RT-PCR results were obtained from samples of febrile subjects originating from Adama Zuria/Ethiopia (1.9%, 2/104), Wolayita Sodo/Ethiopia (1.0%, 1/100), Diego/Madagascar (1.0%, 1/100), and Kintampo/Ghana (1.0%, 1/100), and from samples of non-febrile subjects from Wolayita Sodo/Ethiopia (1%, 2/201). While IgM antibodies against all antigens were identified across all sites, prevalence rates were highest at all Ethiopian sites, albeit in non-febrile populations. Significant correlations in febrile subjects aged p = 0.034) in Adama Zuria/Ethiopia, STY1498 (OR: 3.21, p = 0.008), STY1886 (OR: 2.31, p = 0.054) and STY4539 (OR: 2.82, p = 0.022) in Diego/Madagascar, and STY1498 (OR: 2.45, p = 0.034) in Kintampo/Ghana. We found statistical significance in non-febrile male versus female subjects for STY1498 (OR: 1.96, p = 0.020) in Adama Zuria/Ethiopia, Vi (OR: 2.84, p = 0.048) in Diego/Madagascar, and STY4539 (OR: 0.46, p = 0.009) in Kintampo/Ghana. Conclusions Findings indicate non-discriminatory stages of acute infections, though with site-specific differences. Immune responses among non-febrile, presumably healthy participants may mask recall and/or reporting bias leading to misclassification, or asymptomatic, subclinical infection signs induced by suppression of inflammatory responses. As most Ethiopian participants were ≥ 15 years of age and not at high-risk, the true S. Typhi burden was likely missed. Change-point analyses for generating ELISA proxy cut-offs appeared robust, though misclassification is possible. Our findings provided important information that may be useful to assess sites prior to implementing surveillance for febrile illness including Salmonella disease.
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- 2021
33. Prevalence of Cutaneous Leishmaniasis in Alert Center, Retrospective Analysis, Addis Ababa
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NEWAY, Sebsib, primary, YESHİTELA, Biruk, additional, MEBRAT, Binyam, additional, and AYALEW, Gizeyalew, additional
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- 2021
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34. High Prevalence of Multidrug-Resistant Klebsiella pneumoniae in a Tertiary Care Hospital in Ethiopia
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Awoke, Tewachew, primary, Teka, Brhanu, additional, Seman, Aminu, additional, Sebre, Shemse, additional, Yeshitela, Biruk, additional, Aseffa, Abraham, additional, Mihret, Adane, additional, and Abebe, Tamrat, additional
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- 2021
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35. The genomic epidemiology of multi-drug resistant invasive non-typhoidal Salmonella in selected sub-Saharan African countries
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Park, Se Eun, primary, Pham, Duy Thanh, additional, Pak, Gi Deok, additional, Panzner, Ursula, additional, Maria Cruz Espinoza, Ligia, additional, von Kalckreuth, Vera, additional, Im, Justin, additional, Mogeni, Ondari D., additional, Schütt-Gerowitt, Heidi, additional, Crump, John A, additional, Breiman, Robert F, additional, Adu-Sarkodie, Yaw, additional, Owusu-Dabo, Ellis, additional, Rakotozandrindrainy, Raphaël, additional, Bassiahi Soura, Abdramane, additional, Aseffa, Abraham, additional, Gasmelseed, Nagla, additional, Sooka, Arvinda, additional, Keddy, Karen H, additional, May, Jürgen, additional, Aaby, Peter, additional, Biggs, Holly M, additional, Hertz, Julian T, additional, Montgomery, Joel M, additional, Cosmas, Leonard, additional, Olack, Beatrice, additional, Fields, Barry, additional, Sarpong, Nimako, additional, Razafindrabe, Tsiriniaina Jean Luco, additional, Raminosoa, Tiana Mirana, additional, Kabore, Leon Parfait, additional, Sampo, Emmanuel, additional, Teferi, Mekonnen, additional, Yeshitela, Biruk, additional, El Tayeb, Muna Ahmed, additional, Krumkamp, Ralf, additional, Dekker, Denise Myriam, additional, Jaeger, Anna, additional, Tall, Adama, additional, Gassama, Amy, additional, Niang, Aissatou, additional, Bjerregaard-Andersen, Morten, additional, Løfberg, Sandra Valborg, additional, Deerin, Jessica Fung, additional, Park, Jin Kyung, additional, Konings, Frank, additional, Carey, Megan E, additional, Van Puyvelde, Sandra, additional, Ali, Mohammad, additional, Clemens, John, additional, Dougan, Gordon, additional, Baker, Stephen, additional, and Marks, Florian, additional
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- 2021
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36. The genomic epidemiology of multi-drug resistant invasive non-typhoidal Salmonella in selected sub-Saharan African countries
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Park, Se Eun, Pak, Gi Deok, Panzner, Ursula, Espinoza, Ligia Maria Cruz, von Kalckreuth, Vera, Im, Justin, Mogeni, Ondari D., Schutt-Gerowitt, Heidi, Crump, John A., Breiman, Robert F., Adu-Sarkodie, Yaw, Owusu-Dabo, Ellis, Rakotozandrindrainy, Raphael, Soura, Abdramane Bassiahi, Aseffa, Abraham, Gasmelseed, Nagla, Sooka, Arvinda, Keddy, Karen H., May, Jurgen, Aaby, Peter, Biggs, Holly M., Hertz, Julian T., Montgomery, Joel M., Cosmas, Leonard, Olack, Beatrice, Fields, Barry, Sarpong, Nimako, Razafindrabe, Tsiriniaina Jean Luco, Raminosoa, Tiana Mirana, Kabore, Leon Parfait, Sampo, Emmanuel, Teferi, Mekonnen, Yeshitela, Biruk, El Tayeb, Muna Ahmed, Krumkamp, Ralf, Dekker, Denise Myriam, Jaeger, Anna, Tall, Adama, Gassama, Amy, Niang, Aissatou, Bjerregaard-Andersen, Morten, Lofberg, Sandra Valborg, Deerin, Jessica Fung, Park, Jin Kyung, Konings, Frank, Carey, Megan E., Van Puyvelde, Sandra, Ali, Mohammad, Clemens, John, Dougan, Gordon, Baker, Stephen, Marks, Florian, Park, Se Eun, Pak, Gi Deok, Panzner, Ursula, Espinoza, Ligia Maria Cruz, von Kalckreuth, Vera, Im, Justin, Mogeni, Ondari D., Schutt-Gerowitt, Heidi, Crump, John A., Breiman, Robert F., Adu-Sarkodie, Yaw, Owusu-Dabo, Ellis, Rakotozandrindrainy, Raphael, Soura, Abdramane Bassiahi, Aseffa, Abraham, Gasmelseed, Nagla, Sooka, Arvinda, Keddy, Karen H., May, Jurgen, Aaby, Peter, Biggs, Holly M., Hertz, Julian T., Montgomery, Joel M., Cosmas, Leonard, Olack, Beatrice, Fields, Barry, Sarpong, Nimako, Razafindrabe, Tsiriniaina Jean Luco, Raminosoa, Tiana Mirana, Kabore, Leon Parfait, Sampo, Emmanuel, Teferi, Mekonnen, Yeshitela, Biruk, El Tayeb, Muna Ahmed, Krumkamp, Ralf, Dekker, Denise Myriam, Jaeger, Anna, Tall, Adama, Gassama, Amy, Niang, Aissatou, Bjerregaard-Andersen, Morten, Lofberg, Sandra Valborg, Deerin, Jessica Fung, Park, Jin Kyung, Konings, Frank, Carey, Megan E., Van Puyvelde, Sandra, Ali, Mohammad, Clemens, John, Dougan, Gordon, Baker, Stephen, and Marks, Florian
- Abstract
Background Invasive non-typhoidal Salmonella (iNTS) is one of the leading causes of bacteraemia in sub-Saharan Africa. We aimed to provide a better understanding of the genetic characteristics and transmission patterns associated with multi-drug resistant (MDR) iNTS serovars across the continent. Methods A total of 166 iNTS isolates collected from a multi-centre surveillance in 10 African countries (2010-2014) and a fever study in Ghana (2007-2009) were genome sequenced to investigate the geographical distribution, antimicrobial genetic determinants and population structure of iNTS serotypes-genotypes. Phylogenetic analyses were conducted in the context of the existing genomic frameworks for various iNTS serovars. Population-based incidence of MDR-iNTS disease was estimated in each study site. Results Salmonella Typhimurium sequence-type (ST) 313 and Salmonella Enteritidis ST11 were predominant, and both exhibited high frequencies of MDR; Salmonella Dublin ST10 was identified in West Africa only. Mutations in the gyrA gene (fluoroquinolone resistance) were identified in S. Enteritidis and S. Typhimurium in Ghana; an ST313 isolate carrying bla(CTX-M-15) was found in Kenya. International transmission of MDR ST313 (lineage II) and MDR ST11 (West African clade) was observed between Ghana and neighbouring West African countries. The incidence of MDR-iNTS disease exceeded 100/100 000 person-years-of-observation in children aged Conclusions We identified the circulation of multiple MDR iNTS serovar STs in the sampled sub-Saharan African countries. Investment in the development and deployment of iNTS vaccines coupled with intensified antimicrobial resistance surveillance are essential to limit the impact of these pathogens in Africa.
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- 2021
37. Therapeutic Efficacy of Artemether-Lumefantrine (Coartem®) for the Treatment of Uncomplicated Falciparum Malaria in Africa: A Systematic Review
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Derbie, Awoke, Mekonnen, Daniel, Adugna, Meseret, Yeshitela, Biruk, Woldeamanuel, Yimtubezinash, and Abebe, Tamrat
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Article Subject ,parasitic diseases - Abstract
Background. Africa still bears the largest burden of malaria as the majority of infections in the continent are caused by P. falciparum. Artemether-lumefantrine (AL, Coartem®) is the most widely used artemisinin-based combination therapy (ACT), for treating uncomplicated falciparum malaria globally. However, the development of resistance to antimalarial drugs is a major challenge for malaria control. In this review, the efficacy of AL for the treatment of uncomplicated falciparum malaria in Africa was evaluated. Methods. Articles published between January 2015 and July 2019 were systematically searched using comprehensive search strings from PubMed/Medline, SCOPUS, and grey literature from Google Scholar. Interventional studies that followed patients for at least 28 days were included. Two reviewers independently assessed study eligibility, extracted data, and assessed risk of bias. All the included articles were measured to be good quality. While computing the efficacy of AL, polymerase chain reaction (PCR)–corrected cure rate (adequate clinical and parasitological response, ACPR) at day 28 was considered as the main endpoint. Meta-analysis was computed using STATA v 15 to calculate the pooled ACPR. Results. In this review, 39 articles that reported the treatment outcome of 8,320 patients were included. After 28 days of follow-up, the pooled PCR uncorrected and corrected APCR was at 87% (95% CI: 85-90%) and 97.0% (95% CI: 96-98%), respectively. Moreover, the proportion of early treatment failure (ETF) was almost 0%, while most of the included articles reported
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- 2020
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38. The genomic epidemiology of multi-drug resistant nontyphoidal Salmonella causing invasive disease in sub-Saharan Africa
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Park, Se Eun, primary, Pham, Duy Thanh, additional, Pak, Gi Deok, additional, Panzner, Ursula, additional, Espinoza, Ligia Maria Cruz, additional, von Kalckreuth, Vera, additional, Im, Justin, additional, Mogeni, Ondari D., additional, Schütt-Gerowitt, Heidi, additional, Crump, John A., additional, Breiman, Robert F., additional, Adu-Sarkodie, Yaw, additional, Owusu-Dabo, Ellis, additional, Rakotozandrindrainy, Raphaёl, additional, Soura, Abdramane Bassiahi, additional, Aseffa, Abraham, additional, Gasmelseed, Nagla, additional, Sooka, Arvinda, additional, Keddy, Karen H., additional, May, Jürgen, additional, Aaby, Peter, additional, Biggs, Holly M., additional, Hertz, Julian T., additional, Montgomery, Joel M., additional, Cosmas, Leonard, additional, Olack, Beatrice, additional, Fields, Barry, additional, Sarpong, Nimako, additional, Razafindrabe, Tsiriniaina Jean Luco, additional, Raminosoa, Tiana Mirana, additional, Kabore, Leon Parfait, additional, Sampo, Emmanuel, additional, Teferi, Mekonnen, additional, Yeshitela, Biruk, additional, Tayeb, Muna Ahmed El, additional, Krumkamp, Ralf, additional, Dekker, Denise Myriam, additional, Jaeger, Anna, additional, Tall, Adama, additional, Niang, Aissatou, additional, Bjerregaard-Andersen, Morten, additional, Løfberg, Sandra Valborg, additional, Deerin, Jessica Fung, additional, Park, Jin Kyung, additional, Konings, Frank, additional, Van Puyvelde, Sandra, additional, Ali, Mohammad, additional, Clemens, John D., additional, Dougan, Gordon, additional, Baker, Stephen, additional, and Marks, Florian, additional
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- 2020
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39. Detection of blaKPC and blaNDM carbapenemase genes among Klebsiella pneumoniae isolates in Addis Ababa, Ethiopia: Dominance of blaNDM.
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Awoke, Tewachew, Teka, Brhanu, Aseffa, Abraham, Sebre, Shemse, Seman, Aminu, Yeshitela, Biruk, Abebe, Tamrat, and Mihret, Adane
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CARBAPENEMASE ,KLEBSIELLA pneumoniae ,ERTAPENEM ,MEROPENEM ,DOMINANCE (Genetics) - Abstract
Background: Infections caused by Klebsiella pneumoniae have been difficult to control because of the worldwide emergence of carbapenem-resistant isolates mainly due to carbapenemase production. Information regarding carbapenemase-producing K. pneumoniae is still scarce in Ethiopia. Therefore, the current study aimed to determine the prevalence of carbapenemase-producing K. pneumoniae and to assess the occurrence of bla
NDM and blaKPC carbapenemase genes. Methods: A cross-sectional study was conducted from September 2018 to February 2019 at Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia. A total of 132 non-duplicate K. pneumoniae isolates were studied. Phenotypic confirmation of carbapenemase production was done by modified Carbapenem Inactivation Method (mCIM). Multiplex PCR was performed for the detection of carbapenemase-encoding genes blaKPC , and blaNDM . Results: Out of the total 132 K. pneumoniae isolates, 39 (29.6%) were non-susceptible to one or more carbapenems. The prevalence of carbapenemase-producing isolates from the total was 28 (21.2%) with mCIM of which the most dominant gene was blaNDM 26 (92.9%) and one isolate carried blaKPC concomitantly. Carbapenemase-producing K. pneumoniae isolates were 100% non-susceptible to half of the antimicrobials used in the study, including meropenem and ertapenem. Previous use of carbapenems was associated with carbapenemase production (P = 0.004). Conclusions: The prevalence of carbapenemase-producing K. pneumoniae isolates was worrying in the study area. To our knowledge, the study described the emergence of blaNDM and blaKPC gene carrying K. pneumoniae in Ethiopia for the first time. Further large-scale molecular-based studies, including other carbapenemase genes and sequencing of K. pneumoniae, are warranted to have a clear awareness about the presence of antimicrobial resistance high-risk clones in Ethiopia. [ABSTRACT FROM AUTHOR]- Published
- 2022
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40. Multicountry Distribution and Characterization of Extended-spectrum beta-Lactamase-associated Gram-negative Bacteria From Bloodstream Infections in Sub-Saharan Africa
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Toy, Trevor, Pak, Gi Deok, Campbell, James I., El Tayeb, Muna Ahmed, Von Kalckreuth, Vera, Im, Justin, Panzner, Ursula, Espinoza, Ligia Maria Cruz, Eibach, Daniel, Dekker, Denise Myriam, Park, Se Eun, Jeon, Hyon Jin, Konings, Frank, Mogeni, Ondari D., Cosmas, Leonard, Bjerregaard-Andersen, Morten, Gasmelseed, Nagla, Hertz, Julian T., Jaeger, Anna, Krumkamp, Ralf, Ley, Benedikt, Thriemer, Kamala, Kabore, Leon Parfait, Niang, Aissatou, Raminosoa, Tiana Mirana, Sampo, Emmanuel, Sarpong, Nimako, Soura, Abdramane, Owusu-Dabo, Ellis, Teferi, Mekonnen, Yeshitela, Biruk, Poppert, Sven, May, Juergen, Kim, Jerome H., Chon, Yun, Park, Jin Kyung, Aseffa, Abroaham, Breiman, Robert F., Schuett-Gerowitt, Heidi, Aaby, Peter, Adu-Sarkodie, Yaw, Crump, John A., Rakotozandrindrainy, Raphael, Meyer, Christian G., Sow, Amy Gassama, Clemens, John D., Wierzba, Thomas F., Baker, Stephen, Marks, Florian, Toy, Trevor, Pak, Gi Deok, Campbell, James I., El Tayeb, Muna Ahmed, Von Kalckreuth, Vera, Im, Justin, Panzner, Ursula, Espinoza, Ligia Maria Cruz, Eibach, Daniel, Dekker, Denise Myriam, Park, Se Eun, Jeon, Hyon Jin, Konings, Frank, Mogeni, Ondari D., Cosmas, Leonard, Bjerregaard-Andersen, Morten, Gasmelseed, Nagla, Hertz, Julian T., Jaeger, Anna, Krumkamp, Ralf, Ley, Benedikt, Thriemer, Kamala, Kabore, Leon Parfait, Niang, Aissatou, Raminosoa, Tiana Mirana, Sampo, Emmanuel, Sarpong, Nimako, Soura, Abdramane, Owusu-Dabo, Ellis, Teferi, Mekonnen, Yeshitela, Biruk, Poppert, Sven, May, Juergen, Kim, Jerome H., Chon, Yun, Park, Jin Kyung, Aseffa, Abroaham, Breiman, Robert F., Schuett-Gerowitt, Heidi, Aaby, Peter, Adu-Sarkodie, Yaw, Crump, John A., Rakotozandrindrainy, Raphael, Meyer, Christian G., Sow, Amy Gassama, Clemens, John D., Wierzba, Thomas F., Baker, Stephen, and Marks, Florian
- Abstract
Background. Antimicrobial resistance (AMR) is a major global health concern, yet, there are noticeable gaps in AMR surveillance data in regions such as sub-Saharan Africa. We aimed to measure the prevalence of extended-spectrum beta-lactamase (ESBL) producing Gram-negative bacteria in bloodstream infections from 12 sentinel sites in sub-Saharan Africa. Methods. Data were generated during the Typhoid Fever Surveillance in Africa Program (TSAP), in which standardized blood cultures were performed on febrile patients attending 12 health facilities in 9 sub-Saharan African countries between 2010 and 2014. Pathogenic bloodstream isolates were identified at the sites and then subsequently confirmed at a central reference laboratory. Antimicrobial susceptibility testing, detection of ESBL production, and conventional multiplex polymerase chain reaction (PCR) testing for genes encoding for beta-lactamase were performed on all pathogens. Results. Five hundred and five pathogenic Gram-negative bloodstream isolates were isolated during the study period and available for further characterization. This included 423 Enterobacteriaceae. Phenotypically, 61 (12.1%) isolates exhibited ESBL activity, and genotypically, 47 (9.3%) yielded a PCR amplicon for at least one of the screened ESBL genes. Among specific Gram-negative isolates, 40 (45.5%) of 88 Klebsiella spp., 7 (5.7%) of 122 Escherichia coli, 6 (16.2%) of 37 Acinetobacter spp., and 2 (1.3%) of 159 of nontyphoidal Salmonella (NTS) showed phenotypic ESBL activity. Conclusions. Our findings confirm the presence of ESBL production among pathogens causing bloodstream infections in sub-Saharan Africa. With few alternatives for managing ESBL-producing pathogens in the African setting, measures to control the development and proliferation of AMR organisms are urgently needed.
- Published
- 2019
41. Multicountry Distribution and Characterization of Extended-spectrum β-Lactamase–associated Gram-negative Bacteria From Bloodstream Infections in Sub-Saharan Africa
- Author
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Toy, Trevor, primary, Pak, Gi Deok, additional, Duc, Trung Pham, additional, Campbell, James I, additional, El Tayeb, Muna Ahmed, additional, Von Kalckreuth, Vera, additional, Im, Justin, additional, Panzner, Ursula, additional, Cruz Espinoza, Ligia Maria, additional, Eibach, Daniel, additional, Dekker, Denise Myriam, additional, Park, Se Eun, additional, Jeon, Hyon Jin, additional, Konings, Frank, additional, Mogeni, Ondari D, additional, Cosmas, Leonard, additional, Bjerregaard-Andersen, Morten, additional, Gasmelseed, Nagla, additional, Hertz, Julian T, additional, Jaeger, Anna, additional, Krumkamp, Ralf, additional, Ley, Benedikt, additional, Thriemer, Kamala, additional, Kabore, Leon Parfait, additional, Niang, Aissatou, additional, Raminosoa, Tiana Mirana, additional, Sampo, Emmanuel, additional, Sarpong, Nimako, additional, Soura, Abdramane, additional, Owusu-Dabo, Ellis, additional, Teferi, Mekonnen, additional, Yeshitela, Biruk, additional, Poppert, Sven, additional, May, Jürgen, additional, Kim, Jerome H, additional, Chon, Yun, additional, Park, Jin Kyung, additional, Aseffa, Abroaham, additional, Breiman, Robert F, additional, Schütt-Gerowitt, Heidi, additional, Aaby, Peter, additional, Adu-Sarkodie, Yaw, additional, Crump, John A, additional, Rakotozandrindrainy, Raphaël, additional, Meyer, Christian G, additional, Sow, Amy Gassama, additional, Clemens, John D, additional, Wierzba, Thomas F, additional, Baker, Stephen, additional, and Marks, Florian, additional
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- 2019
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42. Acute Febrile Illness Among Children in Butajira, South–Central Ethiopia During the Typhoid Fever Surveillance in Africa Program
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Teferi, Mekonnen, primary, Desta, Mulualem, additional, Yeshitela, Biruk, additional, Beyene, Tigist, additional, Cruz Espinoza, Ligia Maria, additional, Im, Justin, additional, Jeon, Hyon Jin, additional, Kim, Jong-Hoon, additional, Konings, Frank, additional, Kwon, Soo Young, additional, Pak, Gi Deok, additional, Park, Jin Kyung, additional, Park, Se Eun, additional, Yedenekachew, Melaku, additional, Kim, Jerome, additional, Baker, Stephen, additional, Sir, Won Seok, additional, Marks, Florian, additional, Aseffa, Abraham, additional, and Panzner, Ursula, additional
- Published
- 2019
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43. Bacterial Profile, Antibacterial Resistance Pattern, and Associated Factors from Women Attending Postnatal Health Service at University of Gondar Teaching Hospital, Northwest Ethiopia
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Bitew Kifilie, Abebaw, primary, Dagnew, Mulat, additional, Tegenie, Birhanemeskel, additional, Yeshitela, Biruk, additional, Howe, Rawleigh, additional, and Abate, Ebba, additional
- Published
- 2018
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44. A Seroepidemiological Study of Serogroup A Meningococcal Infection in the African Meningitis Belt
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Manigart, Olivier, Trotter, Caroline, Findlow, Helen, Assefa, Abraham, Mihret, Wude, Moti Demisse, Tesfaye, Yeshitela, Biruk, Osei, Isaac, Hodgson, Abraham, Quaye, Stephen Laryea, Sow, Samba, Coulibaly, Mamadou, Diallo, Kanny, Traore, Awa, Collard, Jean-Marc, Moustapha Boukary, Rahamatou, Djermakoye, Oumarou, Mahamane, Ali Elhaji, Jusot, Jean-François, Sokhna, Cheikh, Alavo, Serge, Doucoure, Souleymane, Ba, El Hadj, Dieng, Mariétou, Diallo, Aldiouma, Daugla, Doumagoum Moto, Omotara, Babatunji, Chandramohan, Daniel, Hassan-King, Musa, Nascimento, Maria, Woukeu, Arouna, Borrow, Ray, Stuart, James M, Greenwood, Brian, London School of Hygiene and Tropical Medicine (LSHTM), Medical Research Council Unit The Gambia (MRC), University of Cambridge [UK] (CAM), Public Health England North West [Manchester] (PHE), Public Health England [London], Armauer Hansen Research Institute (AHRI), Navrongo Health Research Centre [Navrongo, Ghana] (NHRC), Centre pour les Vaccins en Développement [Bamako] (CVD), Centre de Recherche Médicale et Sanitaire (Niamey, Niger) (CERMES), Réseau International des Instituts Pasteur (RIIP), Institut de recherche pour le développement (IRD [Sénégal]), Centre de Support en Santé Internationale [N'Djamena, Tchad] (CSSI), University of Maiduguri, This work was supported by Wellcome Trust (grant no. 086546/Z/08/Z), www.wellcome.ac.uk, Bill & Melinda Gates Foundation (grant no. 51251), www.gatesfoundation.org., Trotter, Caroline [0000-0003-4000-2708], and Apollo - University of Cambridge Repository
- Subjects
Male ,Bacterial Diseases ,Physiology ,lcsh:Medicine ,Meningococcal Disease ,Neisseria meningitidis ,MESH: Africa ,Biochemistry ,Ghana ,MESH: Meningococcal Vaccines ,Geographical Locations ,Seroepidemiologic Studies ,Infectious Diseases of the Nervous System ,[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases ,Immune Physiology ,MESH: Child ,MESH: Antibodies, Bacterial ,Medicine and Health Sciences ,Public and Occupational Health ,Enzyme-Linked Immunoassays ,lcsh:Science ,Child ,MESH: Aged ,MESH: Immunoglobulin G ,Immune System Proteins ,MESH: Middle Aged ,Vaccination ,MESH: Meningitis, Meningococcal ,MESH: Enzyme-Linked Immunosorbent Assay ,Middle Aged ,Antibodies, Bacterial ,Vaccination and Immunization ,MESH: Infant ,Senegal ,Infectious Diseases ,Neurology ,Child, Preschool ,Carrier State ,MESH: Immunologic Memory ,Female ,MESH: Carrier State ,Research Article ,Adult ,Adolescent ,Inflammatory Diseases ,Immunology ,Enzyme-Linked Immunosorbent Assay ,Meningococcal Vaccines ,Meningitis, Meningococcal ,Serogroup ,Research and Analysis Methods ,Antibodies ,MESH: Neisseria meningitidis ,MESH: Cross-Sectional Studies ,Humans ,Meningitis ,Epidemics ,Immunoassays ,MESH: Epidemics ,Aged ,MESH: Adolescent ,MESH: Seroepidemiologic Studies ,MESH: Humans ,lcsh:R ,MESH: Child, Preschool ,Infant ,Biology and Life Sciences ,Proteins ,MESH: Adult ,MESH: Serogroup ,MESH: Vaccination ,[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology ,MESH: Male ,Cross-Sectional Studies ,Immunoglobulin G ,People and Places ,Africa ,Immunologic Techniques ,lcsh:Q ,[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie ,Ethiopia ,Preventive Medicine ,Immunologic Memory ,MESH: Female - Abstract
International audience; The pattern of epidemic meningococcal disease in the African meningitis belt may be influenced by the background level of population immunity but this has been measured infrequently. A standardised enzyme-linked immunosorbent assay (ELISA) for measuring meningococcal serogroup A IgG antibodies was established at five centres within the meningitis belt. Antibody concentrations were then measured in 3930 individuals stratified by age and residence from six countries. Seroprevalence by age was used in a catalytic model to determine the force of infection. Meningococcal serogroup A IgG antibody concentrations were high in each country but showed heterogeneity across the meningitis belt. The geometric mean concentration (GMC) was highest in Ghana (9.09 μg/mL [95% CI 8.29, 9.97]) and lowest in Ethiopia (1.43 μg/mL [95% CI 1.31, 1.57]) on the margins of the belt. The force of infection was lowest in Ethiopia (λ = 0.028). Variables associated with a concentration above the putative protective level of 2 μg/mL were age, urban residence and a history of recent vaccination with a meningococcal vaccine. Prior to vaccination with the serogroup A meningococcal conjugate vaccine, meningococcal serogroup A IgG antibody concentrations were high across the African meningitis belt and yet the region remained susceptible to epidemics.
- Published
- 2016
45. Bacterial etiologic agents causing neonatal sepsis and associated risk factors in Gondar, Northwest Ethiopia
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G/eyesus, Tsehaynesh, primary, Moges, Feleke, additional, Eshetie, Setegn, additional, Yeshitela, Biruk, additional, and Abate, Ebba, additional
- Published
- 2017
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46. The Relationship Between Invasive Nontyphoidal Disease, Other Bacterial Bloodstream Infections, and Malaria in Sub-Saharan Africa
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Park, Se Eun, Pak, Gi Deok, Aaby, Peter, Adu-Sarkodie, Yaw, Ali, Mohammad, Aseffa, Abraham, Biggs, Holly M., Bjerregaard-Andersen, Morten, Breiman, Robert F., Crump, John A., Cruz Espinoza, Ligia Maria, Eltayeb, Muna Ahmed, Gasmelseed, Nagla, Hertz, Julian T., Im, Justin, Jaeger, Anna, Parfait Kabore, Leon, von Kalckreuth, Vera, Keddy, Karen H., Konings, Frank, Krumkamp, Ralf, MacLennan, Calman A., Meyer, Christian G., Montgomery, Joel M., Ahmet Niang, Aissatou, Nichols, Chelsea, Olack, Beatrice, Panzner, Ursula, Park, Jin Kyung, Rabezanahary, Henintsoa, Rakotozandrindrainy, Raphael, Sampo, Emmanuel, Sarpong, Nimako, Schuett-Gerowitt, Heidi, Sooka, Arvinda, Soura, Abdramane Bassiahi, Sow, Amy Gassama, Tall, Adama, Teferi, Mekonnen, Yeshitela, Biruk, May, Juegen, Wierzba, Thomas F., Clemens, John D., Baker, Stephen, Marks, Florian, Park, Se Eun, Pak, Gi Deok, Aaby, Peter, Adu-Sarkodie, Yaw, Ali, Mohammad, Aseffa, Abraham, Biggs, Holly M., Bjerregaard-Andersen, Morten, Breiman, Robert F., Crump, John A., Cruz Espinoza, Ligia Maria, Eltayeb, Muna Ahmed, Gasmelseed, Nagla, Hertz, Julian T., Im, Justin, Jaeger, Anna, Parfait Kabore, Leon, von Kalckreuth, Vera, Keddy, Karen H., Konings, Frank, Krumkamp, Ralf, MacLennan, Calman A., Meyer, Christian G., Montgomery, Joel M., Ahmet Niang, Aissatou, Nichols, Chelsea, Olack, Beatrice, Panzner, Ursula, Park, Jin Kyung, Rabezanahary, Henintsoa, Rakotozandrindrainy, Raphael, Sampo, Emmanuel, Sarpong, Nimako, Schuett-Gerowitt, Heidi, Sooka, Arvinda, Soura, Abdramane Bassiahi, Sow, Amy Gassama, Tall, Adama, Teferi, Mekonnen, Yeshitela, Biruk, May, Juegen, Wierzba, Thomas F., Clemens, John D., Baker, Stephen, and Marks, Florian
- Abstract
Methods.aEuro integral Febrile patients received a blood culture and a malaria test. Isolated bacteria underwent antimicrobial susceptibility testing, and the association between iNTS disease and malaria was assessed. Results.aEuro integral A positive correlation between frequency proportions of malaria and iNTS was observed (P = .01; r = 0.70). Areas with higher burden of malaria exhibited higher odds of iNTS disease compared to other bacterial infections (odds ratio [OR], 4.89; 95% CI, 1.61-14.90; P = .005) than areas with lower malaria burden. Malaria parasite positivity was associated with iNTS disease (OR, 2.44; P = .031) and gram-positive bacteremias, particularly Staphylococcus aureus, exhibited a high proportion of coinfection with Plasmodium malaria. Salmonella Typhimurium and Salmonella Enteritidis were the predominant NTS serovars (53/73; 73%). Both moderate (OR, 6.05; P = .0001) and severe (OR, 14.62; P < .0001) anemia were associated with iNTS disease. Conclusions.aEuro integral A positive correlation between iNTS disease and malaria endemicity, and the association between Plasmodium parasite positivity and iNTS disease across sub-Saharan Africa, indicates the necessity to consider iNTS as a major cause of febrile illness in malaria-holoendemic areas. Prevention of iNTS disease through iNTS vaccines for areas of high malaria endemicity, targeting high-risk groups for Plasmodium parasitic infection, should be considered.
- Published
- 2016
47. Correction: A Seroepidemiological Study of Serogroup A Meningococcal Infection in the African Meningitis Belt
- Author
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Manigart, Olivier, primary, Trotter, Caroline, additional, Findlow, Helen, additional, Aseffa, Abraham, additional, Mihret, Wude, additional, Moti Demisse, Tesfaye, additional, Yeshitela, Biruk, additional, Osei, Isaac, additional, Hodgson, Abraham, additional, Quaye, Stephen Laryea, additional, Sow, Samba, additional, Coulibaly, Mamadou, additional, Diallo, Kanny, additional, Traore, Awa, additional, Collard, Jean-Marc, additional, Moustapha Boukary, Rahamatou, additional, Djermakoye, Oumarou, additional, Mahamane, Ali Elhaji, additional, Jusot, Jean-François, additional, Sokhna, Cheikh, additional, Alavo, Serge, additional, Doucoure, Souleymane, additional, Ba, El Hadj, additional, Dieng, Mariétou, additional, Diallo, Aldiouma, additional, Daugla, Doumagoum Moto, additional, Omotara, Babatunji, additional, Chandramohan, Daniel, additional, Hassan-King, Musa, additional, Nascimento, Maria, additional, Woukeu, Arouna, additional, Borrow, Ray, additional, Stuart, James M., additional, and Greenwood, Brian, additional
- Published
- 2016
- Full Text
- View/download PDF
48. The Relationship Between Invasive NontyphoidalSalmonellaDisease, Other Bacterial Bloodstream Infections, and Malaria in Sub-Saharan Africa
- Author
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Park, Se Eun, primary, Pak, Gi Deok, additional, Aaby, Peter, additional, Adu-Sarkodie, Yaw, additional, Ali, Mohammad, additional, Aseffa, Abraham, additional, Biggs, Holly M., additional, Bjerregaard-Andersen, Morten, additional, Breiman, Robert F., additional, Crump, John A., additional, Cruz Espinoza, Ligia Maria, additional, Eltayeb, Muna Ahmed, additional, Gasmelseed, Nagla, additional, Hertz, Julian T., additional, Im, Justin, additional, Jaeger, Anna, additional, Parfait Kabore, Leon, additional, von Kalckreuth, Vera, additional, Keddy, Karen H., additional, Konings, Frank, additional, Krumkamp, Ralf, additional, MacLennan, Calman A., additional, Meyer, Christian G., additional, Montgomery, Joel M., additional, Ahmet Niang, Aissatou, additional, Nichols, Chelsea, additional, Olack, Beatrice, additional, Panzner, Ursula, additional, Park, Jin Kyung, additional, Rabezanahary, Henintsoa, additional, Rakotozandrindrainy, Raphaël, additional, Sampo, Emmanuel, additional, Sarpong, Nimako, additional, Schütt-Gerowitt, Heidi, additional, Sooka, Arvinda, additional, Soura, Abdramane Bassiahi, additional, Sow, Amy Gassama, additional, Tall, Adama, additional, Teferi, Mekonnen, additional, Yeshitela, Biruk, additional, May, Jürgen, additional, Wierzba, Thomas F., additional, Clemens, John D., additional, Baker, Stephen, additional, and Marks, Florian, additional
- Published
- 2016
- Full Text
- View/download PDF
49. Detection of blaKPC and blaNDM carbapenemase genes among Klebsiella pneumoniae isolates in Addis Ababa, Ethiopia: Dominance of blaNDM.
- Author
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Awoke, Tewachew, Teka, Brhanu, Aseffa, Abraham, Sebre, Shemse, Seman, Aminu, Yeshitela, Biruk, Abebe, Tamrat, and Mihret, Adane
- Subjects
- *
CARBAPENEMASE , *KLEBSIELLA pneumoniae , *ERTAPENEM , *MEROPENEM , *DOMINANCE (Genetics) - Abstract
Background: Infections caused by Klebsiella pneumoniae have been difficult to control because of the worldwide emergence of carbapenem-resistant isolates mainly due to carbapenemase production. Information regarding carbapenemase-producing K. pneumoniae is still scarce in Ethiopia. Therefore, the current study aimed to determine the prevalence of carbapenemase-producing K. pneumoniae and to assess the occurrence of blaNDM and blaKPC carbapenemase genes. Methods: A cross-sectional study was conducted from September 2018 to February 2019 at Tikur Anbessa Specialized Hospital, Addis Ababa, Ethiopia. A total of 132 non-duplicate K. pneumoniae isolates were studied. Phenotypic confirmation of carbapenemase production was done by modified Carbapenem Inactivation Method (mCIM). Multiplex PCR was performed for the detection of carbapenemase-encoding genes blaKPC, and blaNDM. Results: Out of the total 132 K. pneumoniae isolates, 39 (29.6%) were non-susceptible to one or more carbapenems. The prevalence of carbapenemase-producing isolates from the total was 28 (21.2%) with mCIM of which the most dominant gene was blaNDM 26 (92.9%) and one isolate carried blaKPC concomitantly. Carbapenemase-producing K. pneumoniae isolates were 100% non-susceptible to half of the antimicrobials used in the study, including meropenem and ertapenem. Previous use of carbapenems was associated with carbapenemase production (P = 0.004). Conclusions: The prevalence of carbapenemase-producing K. pneumoniae isolates was worrying in the study area. To our knowledge, the study described the emergence of blaNDM and blaKPC gene carrying K. pneumoniae in Ethiopia for the first time. Further large-scale molecular-based studies, including other carbapenemase genes and sequencing of K. pneumoniae, are warranted to have a clear awareness about the presence of antimicrobial resistance high-risk clones in Ethiopia. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
50. High burden of nosocomial infections caused by multi-drug resistant pathogens in pediatric patients at Hawassa University comprehensive specialized hospital
- Author
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Alemayehu, Tsegaye, Tadesse, Endale, Ayalew, Sosina, Nigusse, Bezaye, Yeshitela, Biruk, Amsalu, Anteneh, and Aseffa, Abraham
- Subjects
drug resistance ,pediatrics ,Ethiopia ,cross infection ,patients - Abstract
Background: Pediatric patients are at increased risk of nosocomial infections with multi-drug resistant pathogens which are more prevalent in the hospital environment. The aim of this study was to determine the prevalence of nosocomial infections, antibiotic resistance pattern of bacterial isolates and associated factors in pediatric patients. Methods: This was a cross-sectional study conducted from March to August, 2016. Data included sociodemographic and clinical variables in patients clinically suspected of having nosocomial infections and collected using a structured questionnaire. Bacterial identification and antimicrobial susceptibility test was done with standard microbiological methods. Data were analyzed with SPSS version 20 and p value < 0.05 was considered to be statistically significant. Results: Out of 939 children admitted to the hospital, 384 patients (40.9%) were clinically suspected and had 462 nosocomial infections. Blood stream infection was the most common site of nosocomial infections. Culture confirmed nosocomial infections were reported in 82 patients (21.4%; 95% CI, 17.2-25.8%) with 88 isolates. Among the most frequently isolated bacteria, 21 (23.9%) were Klebsiella spp, and 16 (18.2%) were S. aureus, 62.5% of which were methicillin resistant. Among all bacterial pathogens, 88.9% were multi-drug resistant. Extremely high (97.9%) multi-drug resistance was associated with Gram negative bacteria. Among these, all isolates, except E. coli, were 100% multi-drug resistant. Long hospital stay and malnutrition were significantly associated with nosocomial infections. Conclusion: The high prevalence of nosocomial infections with multi-drug resistant bacteria suggests the need for proper implementation of the nosocomial infections prevention and control measures Refereed/Peer-reviewed
- Published
- 2019
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