17 results on '"Yeqi Fu"'
Search Results
2. Greybox Fuzzing Based on Ant Colony Algorithm.
- Author
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Bowen Sun, Bo Wang, Baojiang Cui, and Yeqi Fu
- Published
- 2020
- Full Text
- View/download PDF
3. Establishment of a Tm-shift Method for Detection of Cat-Derived Hookworms
- Author
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Xue Zhou, Guoqing Li, Xiu Li, Long He, Asmaa M.I. Abuzeid, Rongkun Ran, Jumei Liu, Yunqiu Liu, Yeqi Fu, Yue Huang, and Qi Zhao
- Subjects
Ancylostoma ,Coefficient of variation ,Melting temperature ,ITS1 ,Anoylostoma ceylanicum ,cat ,SNP ,Cat Diseases ,Real-Time Polymerase Chain Reaction ,Sensitivity and Specificity ,Melting curve analysis ,Ancylostomiasis ,Feces ,chemistry.chemical_compound ,DNA, Ribosomal Spacer ,Animals ,Transition Temperature ,Internal transcribed spacer ,Anoylostoma tubaeforme ,DNA Primers ,Ancylostoma ceylanicum ,Chromatography ,biology ,Dna concentration ,Reproducibility of Results ,DNA, Helminth ,biology.organism_classification ,Standard curve ,Infectious Diseases ,Molecular Diagnostic Techniques ,chemistry ,Cats ,SYBR Green I ,Original Article ,Parasitology ,Tm-shift - Abstract
Melting temperature shift (Tm-shift) is a new detection method that analyze the melting curve on real-time PCR thermocycler using SYBR Green I fluorescent dye. To establish a Tm-shift method for the detection of Ancylostoma ceylanicum and A. tubaeforme in cats, specific primers, with GC tail of unequal length attached to their 5 ́ end, were designed based on 2 SNP loci (ITS101 and ITS296) of the internal transcribed spacer 1 (ITS1) sequences. The standard curve of Tm-shift was established using the standard plasmids of A. ceylanicum (AceP) and A. tubaeforme (AtuP). The Tm-shift method stability, sensitivity, and accuracy were tested with reference to the standard curve, and clinical fecal samples were also examined. The results demonstrated that the 2 sets of primers based on the 2 SNPs could accurately distinguish between A. ceylanicum and A. tubaeforme. The coefficient of variation (CV) of Tm-values of AceP and AtuP was 0.07% and 0.06% in ITS101 and was 0.06% and 0.08% in ITS296, respectively. The minimum detectable DNA concentration was 5.22×10-6 and 5.28×10-6 ng/μl samples of AceP and AtuP, respectively. The accuracy of Tm-shift method reached 100% based on examination of 10 hookworm DNA samples with known species. In the clinical detection of hookworm in 69 stray cat fecal sample, the Tm-shift detection results were consistent with the microscopic examination and successfully differentiated between the 2-hookworm species. In conclusion, the developed method is a rapid, sensitive and accurate technique and can provide a promising tool for clinical detection and epidemiological investigation of cat-derived hookworms.
- Published
- 2019
4. Prevalence and genotypes of Giardia lamblia from stray dogs and cats in Guangdong, China
- Author
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Fang Yang, Xianli Shi, Pan Zhang, Weida Pan, Yeqi Fu, Auwalu Yusuf Abdullahi, Jianxiong Hang, Mingwei Wang, Guoqing Li, and Xinxin Yan
- Subjects
Giardiasis ,Male ,0301 basic medicine ,China ,Veterinary medicine ,Genotype ,030231 tropical medicine ,Protozoan Proteins ,Intestinal parasite ,Biology ,Cat Diseases ,medicine.disease_cause ,Polymerase Chain Reaction ,law.invention ,Feces ,03 medical and health sciences ,Dogs ,0302 clinical medicine ,law ,parasitic diseases ,Prevalence ,RNA, Ribosomal, 18S ,medicine ,Animals ,Giardia lamblia ,Dog Diseases ,Phylogeny ,Polymerase chain reaction ,CATS ,General Veterinary ,Phylogenetic tree ,Sequence Analysis, DNA ,DNA, Protozoan ,030108 mycology & parasitology ,Cytoskeletal Proteins ,Diarrhea ,Cats ,Female ,Parasitology ,medicine.symptom ,Triose-Phosphate Isomerase - Abstract
Giardia lamblia is a worldwide zoonotic intestinal parasite that infects humans and a wide range of mammals including dogs and cats, causing giardiasis with diarrhea. To investigate the infection and distribution of G. lamblia genotypes from stray dogs and cats in Guangdong, China according to different districts, gender and ages, fecal samples were collected and examined by microscopy, and all isolates were genotyped by PCR amplification using beta-giardin (bg) and triose phosphate isomerase (tpi) genes as molecular markers. The results showed that the prevalence of dogs and cats was 10.8% (57/527) and 5.8% (6/104), respectively. Sixty-one samples were detected by microscopy and 63 were amplified and successfully sequenced by the PCR. Based on the phylogenetic analysis, 25 canine isolates (24 assemblages AI and 1 assemblage D) were genotyped by tpi gene and 57 canine isolates (26 assemblages AI, 18 assemblages C and 13 assemblages D) genotyped by bg gene; 6 feline isolates were identified as assemblage AI by tpi gene, and as 3 assemblages AI and 3 assemblages F by bg gene. The dominant genotypes were assemblage AI in younger dogs (assemblage C in adult dogs) and assemblage C in male dogs (assemblage AI in female dogs). Mixed genotype infections were found in different age and gender groups. The results indicated that G. lamblia from stray dogs and cats in Guangdong province had a zoonotic potential with assemblage AI as the prevalent genotype. The different risk factors (age and sex) may have an impact on the infection of different genotypes.
- Published
- 2018
5. New record of Ascaridia nymphii (Secernentea: Ascaridiidae) from macaw parrot, Ara chloroptera, in China
- Author
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M W Wang, Xinxin Yan, Guoqing Li, Xianli Shi, Fang Yang, Pan Zhang, Kangxin Li, Yeqi Fu, and Jianxiong Hang
- Subjects
Male ,0301 basic medicine ,China ,040301 veterinary sciences ,Zoology ,Biology ,Ascaridia ,DNA, Ribosomal ,Secernentea ,0403 veterinary science ,Macaw ,Feces ,03 medical and health sciences ,Monophyly ,Parrots ,parasitic diseases ,Sucker ,Animals ,Ascaridiasis ,Clade ,Phylogeny ,Electron Transport Complex I ,Phylogenetic tree ,Bird Diseases ,04 agricultural and veterinary sciences ,biology.organism_classification ,Ara chloroptera ,030104 developmental biology ,Infectious Diseases ,DNA, Intergenic ,Female ,Parasitology - Abstract
Present study was performed to identify the species of ascarids from macaw parrot, Ara chloroptera, in China. Total 6 ascarids (3 males and 3 females) were collected in the feces of 3 macaws at Guangzhou Zoo in Guangdong Province, China. Their morphological characteristics with dimensions were observed under a light microscope, and their genetic characters were analyzed with the partial 18S rDNA, ITS rDNA and nad4 gene sequences, respectively. Results showed that all worms have no interlabia but male worms have two alate spicules, well-developed precloacal sucker and a tail with ventrolateral caudal alae and 11 pairs of papillae. The partial 18S rDNA, ITS rDNA and nad4 sequences were 831bp, 1015bp and 394bp in length, respectively. They showed the highest similarity of 99.8% (18S rDNA) with Ascaridia nymphii, 93.8% identities (ITS rDNA) with A. columbae and 98.5% to 99.5% identities (nad4) with Ascaridia sp. from infected parrot. All Ascaridia nematodes from the macaws were clustered into one clade and formed monophyletic group of Ascaridia with A. columbae and A. galli in two phylogenetic trees. It is observed that the combining morphological and sequencing data from three loci, the present Ascaridia species was identified as Ascaridia nymphii, which is the first record of A. nymphii from macaw parrot in China.
- Published
- 2018
6. Tm-Shift Detection of Dog-Derived Ancylostoma ceylanicum and A. caninum
- Author
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Pan Zhang, Yunqiu Liu, Auwalu Yusuf Abdullahi, Rongkun Ran, Guoqing Li, Mingwei Wang, Yeqi Fu, Jianxiong Hang, Yue Huang, Xinxin Yan, and Yongxiang Sun
- Subjects
0301 basic medicine ,General Immunology and Microbiology ,biology ,Coefficient of variation ,030231 tropical medicine ,Helminth genetics ,General Medicine ,biology.organism_classification ,Ancylostoma species ,Molecular biology ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,Intergenic region ,Helminths ,Internal transcribed spacer ,Feces ,Ancylostoma ceylanicum - Abstract
To develop a Tm-shift method for detection of dog-derived Ancylostoma ceylanicum and A. caninum, three sets of primers were designed based on three SNPs (ITS71, ITS197, and ITS296) of their internal transcribed spacer 1 (ITS1) sequences. The detection effect of the Tm-shift was assessed through the stability, sensitivity, accuracy test, and clinical detection. The results showed that these three sets of primers could distinguish accurately between A. ceylanicum and A. caninum. The coefficient of variation in their Tm values on the three SNPs was 0.09% and 0.15% (ITS71), 0.18% and 0.14% (ITS197), and 0.13% and 0.07% (ITS296), respectively. The lowest detectable concentration of standard plasmids for A. ceylanicum and A. caninum was 5.33 × 10−6 ng/μL and 5.03 × 10−6 ng/μL. The Tm-shift results of ten DNA samples from the dog-derived hookworms were consistent with their known species. In the clinical detection of 50 fecal samples from stray dogs, the positive rate of hookworm detected by Tm-shift (42%) was significantly higher than that by microscopic examination (34%), and the former can identify the Ancylostoma species. It is concluded that the Tm-shift method is rapid, specific, sensitive, and suitable for the clinical detection and zoonotic risk assessment of the dog-derived hookworm.
- Published
- 2018
7. The mitochondrial genome ofAncylostoma tubaeformefrom cats in China
- Author
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Xianli Shi, Xinxin Yan, M W Wang, Fang Yang, X.G. Yu, Weida Pan, Yeqi Fu, Jianxiong Hang, Pan Zhang, Guoqing Li, and Auwalu Yusuf Abdullahi
- Subjects
0301 basic medicine ,China ,Veterinary medicine ,Ancylostoma ,Helminth genetics ,Biology ,Cat Diseases ,DNA, Mitochondrial ,Genome ,Ancylostomiasis ,03 medical and health sciences ,Ancylostoma tubaeforme ,RNA, Transfer ,Animals ,Internal transcribed spacer ,Gene ,Phylogeny ,Whole genome sequencing ,Genetics ,General Medicine ,DNA, Helminth ,Ribosomal RNA ,biology.organism_classification ,030104 developmental biology ,RNA, Ribosomal ,GenBank ,Genome, Mitochondrial ,Cats ,Animal Science and Zoology ,Parasitology ,RNA, Helminth - Abstract
Ancylostoma tubaeformemay infect canids, felids and humans, and pose a potential risk to public health. Polymerase chain reaction (PCR) techniques were used to amplify the complete mitochondrial (mt) genome sequence ofA. tubaeformefrom cats and to analyse its sequence characteristics after molecular identification based on the internal transcribed spacer ITS1+ sequence. The results show that the complete mt genome sequence (GenBank accession number KY070315) ofA. tubaeformefrom cats was 13,730 bp in length, including 12 protein-coding genes, 22 transfer RNA (tRNA) genes, two ribosomal RNA (rRNA) genes, two non-coding regions and an AT-rich region. The nucleotide content of A and T was 77.93%, biased toward A and T. Twelve protein-coding genes used ATT, TTG and GTG as initiation codons, and TAA, TAG, TA and T as termination codons. The length of the 22 tRNA genes ranged from 52 to 62 bp, their predicted secondary structures were D loops and V loops. The lengths of the two rRNAs were 958 and 697 bp. Phylogenetic analyses showed thatA. tubaeformefrom cats was in the lineage ofAncylostoma, having a close phylogenetic relationship withA. caninum. This study reports for the first time the mt genome ofA. tubaeformefrom cats in China, which could enhance the mt genome database of Ancylostomatidae nematodes, and it offers the scientific basis for further studies in the genetic diversity of hookworms among different hosts.
- Published
- 2017
8. Development of T m -shift genotyping method for detection of cat-derived Giardia lamblia
- Author
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Yeqi Fu, Fang Yang, Pan Zhang, Xingang Yu, Jianxiong Hang, Weida Pan, Xinxin Yan, Mingwei Wang, Xianli Shi, Guoqing Li, and Auwalu Yusuf Abdullahi
- Subjects
0301 basic medicine ,General Veterinary ,Coefficient of variation ,Pcr cloning ,Single-nucleotide polymorphism ,General Medicine ,Biology ,medicine.disease_cause ,Molecular biology ,Melting curve analysis ,03 medical and health sciences ,030104 developmental biology ,Infectious Diseases ,Insect Science ,Genotype ,medicine ,Giardia lamblia ,Parasitology ,Detection rate ,Genotyping - Abstract
To develop T m -shift genotyping method for detection of cat-derived Giardia lamblia, two sets of primers with two GC-rich tails of unequal length attached to their 5′-end were designed according to two SNPs (BG434 and BG170) of β-giardin (bg) gene, and specific PCR products were identified by inspection of a melting curve on real-time PCR thermocycler. A series of experiments on the stability, sensitivity, and accuracy of T m -shift method was tested, and clinical samples were also detected. The results showed that two sets of primers based on SNP could distinguish accurately between assemblages A and F. Coefficient of variation of T m values of assemblage A and F was 0.14 and 0.07% in BG434 and 0.10 and 0.11% in BG170, respectively. The lowest detection concentration was 4.52 × 10−5 and 4.88 × 10−5 ng/μL samples of assemblage A and F standard plasmids. The T m -shift genotyping results of ten DNA samples from the cat-derived G. lamblia were consistent with their known genotypes. The detection rate of clinical samples by T m -shift was higher than that by microscopy, and their genotyping results were in complete accordance with sequencing results. It is concluded that the T m -shift genotyping method is rapid, specific, and sensitive and may provide a new technological mean for molecular detection and epidemiological investigation of the cat-derived G. lamblia.
- Published
- 2017
9. Sequence Analysis of Mitochondrial Genome of Toxascaris leonina from a South China Tiger
- Author
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M W Wang, Auwalu Yusuf Abdullahi, Meiran Song, Yeqi Fu, Guoqing Li, Xianli Shi, Wu Chen, Fang Yang, Fang Shan, Weida Pan, and Kangxin Li
- Subjects
0301 basic medicine ,Genetics ,education.field_of_study ,Toxascaris leonina ,biology ,030231 tropical medicine ,Population ,biology.organism_classification ,South China tiger ,Genome ,Stop codon ,03 medical and health sciences ,Paleontology ,030104 developmental biology ,0302 clinical medicine ,Infectious Diseases ,Intergenic region ,Start codon ,Parasitology ,education ,Gene - Abstract
Toxascaris leonina is a common parasitic nematode of wild mammals and has significant impacts on the protection of rare wild animals. To analyze population genetic characteristics of T. leonina from South China tiger, its mitochondrial (mt) genome was sequenced. Its complete circular mt genome was 14,277 bp in length, including 12 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 2 non-coding regions. The nucleotide composition was biased toward A and T. The most common start codon and stop codon were TTG and TAG, and 4 genes ended with an incomplete stop codon. There were 13 intergenic regions ranging 1 to 10 bp in size. Phylogenetically, T. leonina from a South China tiger was close to canine T. leonina. This study reports for the first time a complete mt genome sequence of T. leonina from the South China tiger, and provides a scientific basis for studying the genetic diversity of nematodes between different hosts.
- Published
- 2016
10. The mitochondrial genome of Dipetalonema gracile from a squirrel monkey in China
- Author
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Yeqi Fu, Xianli Shi, Y Huang, Jianxiong Hang, W Chen, Pan Zhang, Y X Sun, Guoqing Li, M W Wang, Xinxin Yan, Y Q Liu, R K Ran, and Auwalu Yusuf Abdullahi
- Subjects
Genetics ,0303 health sciences ,Mitochondrial DNA ,biology ,Sequence analysis ,030231 tropical medicine ,Squirrel monkey ,General Medicine ,Ribosomal RNA ,biology.organism_classification ,Dipetalonema ,Stop codon ,030308 mycology & parasitology ,03 medical and health sciences ,0302 clinical medicine ,Transfer RNA ,Animal Science and Zoology ,Parasitology ,Gene - Abstract
Dipetalonema gracile is a common parasite in squirrel monkeys (Saimiri sciureus), which can cause malnutrition and progressive wasting of the host, and lead to death in the case of massive infection. This study aimed to identify a suspected D. gracile worm from a dead squirrel monkey by means of molecular biology, and to amplify its complete mitochondrial genome by polymerase chain reaction (PCR) and sequence analysis. The results identified the worm as D. gracile, and the full length of its complete mitochondrial genome was 13,584 bp, which contained 22 tRNA genes, 12 protein-coding genes, two rRNA genes, one AT-rich region and one small non-coding region. The nucleotide composition included A (16.89%), G (20.19%), T (56.22%) and C (6.70%), among which A + T = 73.11%. The 12 protein-coding genes used TTG and ATT as start codons, and TAG and TAA as stop codons. Among the 22 tRNA genes, only trnS1AGN and trnS2UCN exhibited the TΨC-loop structure, while the other 20 tRNAs showed the TV-loop structure. The rrnL (986 bp) and rrnS (685 bp) genes were single-stranded and conserved in secondary structure. This study has enriched the mitochondrial gene database of Dipetalonema and laid a scientific basis for further study on classification, and genetic and evolutionary relationships of Dipetalonema nematodes.
- Published
- 2018
11. Development of multi-ARMS-qPCR method for detection of hookworms from cats and dogs
- Author
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Rongkun Ran, Yeqi Fu, Jianxiong Hang, Asmaa M.I. Abuzeid, Yunqiu Liu, Guoqing Li, Yue Huang, Pan Zhang, Chenyang Huo, Yongxiang Sun, Xinxin Yan, and Mingwei Wang
- Subjects
0301 basic medicine ,Ancylostoma ,030231 tropical medicine ,Cat Diseases ,Real-Time Polymerase Chain Reaction ,Sensitivity and Specificity ,Ancylostomiasis ,03 medical and health sciences ,Dogs ,0302 clinical medicine ,parasitic diseases ,Genotype ,medicine ,Animals ,Helminths ,Dog Diseases ,Genotyping ,Feces ,Ancylostoma ceylanicum ,CATS ,biology ,Reproducibility of Results ,030108 mycology & parasitology ,biology.organism_classification ,Virology ,Diarrhea ,Infectious Diseases ,Parasitology ,Mutation ,Cats ,medicine.symptom ,Nucleic Acid Amplification Techniques - Abstract
Hookworms are blood-sucking nematodes that infect dogs, cats, and humans, causing iron-deficiency anemia, abdominal pain, diarrhea, and skin inflammation. Amplification refractory mutation system (ARMS) is a modified technology based on allele-specific PCR, which is widely used in mutation detection and genotyping. However, no data about ARMS application in hookworm detection. This study aims to establish a multi-ARMS-qPCR method for the detection of three hookworm species from dogs and cats. A universal forward primer and three specific primers (ARMS-Cey, ARMS-Can, and ARMS-Tub) were designed based on the three ITS SNPs (ITS250, ITS78 and ITS153) of Ancylostoma ceylanicum, A. caninum, and A. tubaeforme, respectively. The results showed that the three designed ARMS primers generated specific melting curves for the three hookworms' standard plasmids. The melting temperature (Tm) values were 88.40 °C (A. ceylanicum), 83.15 °C (A. caninum), and 85.65 °C (A. tubaeforme), with good reproducibility of intra- and inter-assay. No amplification was observed with other intestinal parasites. The limit of detection using the established technique was 1, 2, and 104 egg per gram feces (EPG) for A. caninum, A. tubaeforme and A. ceylanicum, respectively. Using multi-ARMS-qPCR assay, 17 out of 50 fecal samples were positive for hookworms, including ten single and seven mixed infections, and single infections were quantified. In conclusion, the used multi-ARMS-qPCR method has the advantages of high efficiency, sensitivity, specificity, and quantitative analysis and can be used for the clinical detection, epidemiological investigation, and zoonotic risk assessment of canine and feline hookworms.
- Published
- 2019
12. Molecular differentiation of three canine and feline hookworms in South China through HRM analysis
- Author
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Fang Yang, Guoqing Li, Pan Zhang, Yeqi Fu, Weida Pan, Jianxiong Hang, M W Wang, Xianli Shi, and Xinxin Yan
- Subjects
0301 basic medicine ,Ancylostomatoidea ,China ,South china ,Melting temperature ,030231 tropical medicine ,Helminth genetics ,Biology ,Cat Diseases ,DNA, Ribosomal ,Polymerase Chain Reaction ,03 medical and health sciences ,Feces ,Hookworm Infections ,0302 clinical medicine ,Dogs ,Limit of Detection ,Prevalence ,Animals ,Transition Temperature ,Dog Diseases ,Internal transcribed spacer ,Phylogeny ,Ancylostoma ceylanicum ,DNA Primers ,Reproducibility of Results ,General Medicine ,030108 mycology & parasitology ,DNA, Helminth ,biology.organism_classification ,Virology ,Cats ,Animal Science and Zoology ,Parasitology ,Polymorphism, Restriction Fragment Length ,Mixed infection - Abstract
To investigate the prevalence of canine and feline hookworms in South China, and to assess the risk of zoonotic hookworms to humans, one pair of primers (HRM-F/HRM-R) was designed to establish a high-resolution melting (HRM) method based on internal transcribed spacer 1 (ITS-1) rDNA for the detection of Ancylostoma ceylanicum, A. caninum and A. tubaeforme infection. The results showed that the HRM for the three hookworms produced different melting-curve profiles, where melting temperature (Tm) values were 84.50°C for A. ceylanicum, 82.25°C for A. caninum and 81.73°C for A. tubaeforme, respectively. The reproducibility of intra- and inter-assay melting curves was almost perfect. The lowest concentration detected was about 5.69 ×10−4 g/μl. The HRM detection results from 18 canine and feline hookworm samples were in complete accordance with their sequencing results. The HRM method was more sensitive than the polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) technique in the detection of 98 clinical samples. It is concluded that the HRM method can differentiate between A. ceylanicum, A. caninum, A. tubaeforme and their mixed infections, which may provide important technical support for the zoonotic risk assessment and molecular epidemiological survey of canine and feline hookworms.
- Published
- 2018
13. Comparative analysis of Ancylostoma ceylanicum mitochondrial genome with other Ancylostoma species
- Author
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Auwalu Yusuf Abdullahi, Xinxin Yan, Yeqi Fu, Fang Yang, Jianxiong Hang, Pan Zhang, Mingwei Wang, Xianli Shi, Weida Pan, Xingang Yu, and Guoqing Li
- Subjects
0301 basic medicine ,Microbiology (medical) ,Mitochondrial DNA ,Ancylostoma ,Microbiology ,DNA, Mitochondrial ,Evolution, Molecular ,03 medical and health sciences ,Ancylostoma tubaeforme ,RNA, Transfer ,Species Specificity ,parasitic diseases ,Genetics ,Animals ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,Phylogeny ,Ancylostoma ceylanicum ,biology ,Phylogenetic tree ,Ancylostomatidae ,fungi ,DNA, Helminth ,biology.organism_classification ,Biological Evolution ,030104 developmental biology ,Infectious Diseases ,Ancylostoma duodenale ,Genome, Mitochondrial ,Ancylostoma caninum - Abstract
Ancylostoma ceylanicum may inhabit the small intestine of canids, felids and humans, can pose a potential risk to public health. This study is the first time to amplify complete mitochondrial genome sequence of A. ceylanicum from dog and to compare it with Ancylostoma tubaeforme, Ancylostoma duodenale and Ancylostoma caninum. The results showed that the complete mitochondrial genome of A. ceylanicum was 13,660 bp in length, including 12 protein-coding genes, 2 rRNA genes and 22 tRNA genes and 3 non-coding regions (AT-rich region, SNCR and LNCR). Its mtDNA was the shortest, biased toward A and T at base composition, and higher than other three Ancylostoma species at total AT content. Its nad5 and nad6 genes used TTG and ATT as initiation codons, while other three Ancylostoma species used ATT and GTG or ATG. The 22 tRNA genes were different in length among four Ancylostoma species, but their anticodons were the same. Among 12 protein-coding genes, the cox1 gene was the lowest at AT content and minimum at Ka/Ks while the nad2 gene was the opposite. The phylogenetic tree showed that in the lineage of Ancylostoma, A. ceylanicum occurred on a branch external to other three Ancylostoma species, and A. caninum and A. tubaeforme had closer phylogenetic relationship than A. duodenale. This study not only enhances the mitochondrial genome database of Ancylostomatidae nematodes, but also provides new data for further phylogenetic studies among Ancylostomatidae nematodes.
- Published
- 2017
14. Development of T
- Author
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Weida, Pan, Yeqi, Fu, Auwalu Yusuf, Abdullahi, Mingwei, Wang, Xianli, Shi, Fang, Yang, Xingang, Yu, Xinxin, Yan, Pan, Zhang, Jianxiong, Hang, and Guoqing, Li
- Subjects
Giardiasis ,Genotype ,Genotyping Techniques ,Protozoan Proteins ,DNA, Protozoan ,Cat Diseases ,Real-Time Polymerase Chain Reaction ,Polymorphism, Single Nucleotide ,Cytoskeletal Proteins ,Cats ,Animals ,Humans ,Giardia lamblia ,DNA Primers - Abstract
To develop T
- Published
- 2016
15. Prevalence and potential zoonotic risk of hookworms from stray dogs and cats in Guangdong, China
- Author
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Rongkun Ran, Yeqi Fu, Yue Huang, Jianxiong Hang, Yunqiu Liu, Pan Zhang, Yongxiang Sun, Xinxin Yan, Guoqing Li, Asmaa M.I. Abuzeid, and Mingwei Wang
- Subjects
Ancylostomatoidea ,Male ,Risk ,China ,Veterinary medicine ,South china ,Cat Diseases ,Polymerase Chain Reaction ,Sensitivity and Specificity ,Feces ,Hookworm Infections ,Dogs ,Zoonoses ,parasitic diseases ,Prevalence ,Animals ,Dog Diseases ,Hookworm infection ,Ancylostoma ceylanicum ,CATS ,General Veterinary ,biology ,Potential risk ,Age Factors ,DNA, Helminth ,biology.organism_classification ,Infection rate ,Stray cats ,Cats ,Female ,Parasitology ,Mixed infection - Abstract
Hookworm infection is globally prevalent among dogs and cats representing a major public health risk. Although previous studies have surveyed canine and feline hookworms in Guangzhou city, the status of these infection needs to be further explored in other regions of South China. To investigate the prevalence and zoonotic risk of canine and feline hookworms in eight cities (Guangzhou, Foshan, Shenzhen, Huizhou, Zhongshan, Shaoguan, Shantou and Chaozhou) of Guangdong province, China, we developed specific PCR methods based on ITS sequence for identifying three common hookworm species. The results showed that the prevalence of hookworms from stray dogs and cats was 20.23% (142/702) and 15.26% (47/308), respectively. The established PCR methods could identify Ancylostoma ceylanicum, A. caninum and A. tubaeforme. The mixed infections of A. caninum and A. ceylanicum were detected in stray dogs of Guangzhou and Shaoguan, with the rate of 8.3% and 21.2%, respectively. Among the stray dogs in Foshan, the infection rate of A. ceylanicum was higher than that of A. caninum. The stray cats in four of five investigated cities were infected with A. ceylanicum. The different region, age and rearing environments had an impact on the hookworm infection rates of stray dogs and cats. In conclusion, the reported higher infection rate of A. ceylanicum than other hookworm species in stray dogs and cats poses a potential risk to public health.
- Published
- 2019
16. Sequence Analysis of Mitochondrial Genome of
- Author
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Kangxin, Li, Fang, Yang, A Y, Abdullahi, Meiran, Song, Xianli, Shi, Minwei, Wang, Yeqi, Fu, Weida, Pan, Fang, Shan, Wu, Chen, and Guoqing, Li
- Subjects
Male ,Toxascariasis ,Base Composition ,China ,Sequence Homology ,Sequence Analysis, DNA ,Brief Communication ,phylogeny ,Toxascaris ,Genes, Mitochondrial ,mitochondrial genome ,Genome, Mitochondrial ,Animals ,Cluster Analysis ,DNA, Intergenic ,Toxascaris leonina ,Tigers ,South China tiger ,Genes, Helminth - Abstract
Toxascaris leonina is a common parasitic nematode of wild mammals and has significant impacts on the protection of rare wild animals. To analyze population genetic characteristics of T. leonina from South China tiger, its mitochondrial (mt) genome was sequenced. Its complete circular mt genome was 14,277 bp in length, including 12 protein-coding genes, 22 tRNA genes, 2 rRNA genes, and 2 non-coding regions. The nucleotide composition was biased toward A and T. The most common start codon and stop codon were TTG and TAG, and 4 genes ended with an incomplete stop codon. There were 13 intergenic regions ranging 1 to 10 bp in size. Phylogenetically, T. leonina from a South China tiger was close to canine T. leonina. This study reports for the first time a complete mt genome sequence of T. leonina from the South China tiger, and provides a scientific basis for studying the genetic diversity of nematodes between different hosts.
- Published
- 2016
17. Establishment of a Tm-shift Method for Detection of Cat-Derived Hookworms.
- Author
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Yeqi Fu, Yunqiu Liu, Abuzeid, Asmaa M. I., Yue Huang, Xue Zhou, Long He, Qi Zhao, Xiu Li, Jumei Liu, Rongkun Ran, and Guoqing Li
- Subjects
HOOKWORMS ,THERMOCYCLING ,POLYMERASE chain reaction ,ANCYLOSTOMA ,SINGLE nucleotide polymorphisms - Abstract
Melting temperature shift (Tm-shift) is a new detection method that analyze the melting curve on real-time PCR thermocycler using SYBR Green I fluorescent dye. To establish a Tm-shift method for the detection of Ancylostoma ceylanicum and A. tubaeforme in cats, specific primers, with GC tail of unequal length attached to their 5' end, were designed based on 2 SNP loci (ITS101 and ITS296) of the internal transcribed spacer 1 (ITS1) sequences. The standard curve of Tm-shift was established using the standard plasmids of A. ceylanicum (AceP) and A. tubaeforme (AtuP). The Tm-shift method stability, sensitivity, and accuracy were tested with reference to the standard curve, and clinical fecal samples were also examined. The results demonstrated that the 2 sets of primers based on the 2 SNPs could accurately distinguish between A. ceylanicum and A. tubaeforme. The coefficient of variation (CV) of Tm- values of AceP and AtuP was 0.07% and 0.06% in ITS101 and was 0.06% and 0.08% in ITS296, respectively. The minimum detectable DNA concentration was 5.22×10
-6 and 5.28×10-6 ng/µl samples of AceP and AtuP, respectively. The accuracy of Tm-shift method reached 100% based on examination of 10 hookworm DNA samples with known species. In the clinical detection of hookworm in 69 stray cat fecal sample, the Tm-shift detection results were consistent with the microscopic examination and successfully differentiated between the 2-hookworm species. In conclusion, the developed method is a rapid, sensitive and accurate technique and can provide a promising tool for clinical detection and epidemiological investigation of cat-derived hookworms. [ABSTRACT FROM AUTHOR]- Published
- 2019
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