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Abstract Background As calcium silicate-based cements (CSCs) have found success in various vital pulp therapy applications, several new CSC products have emerged. This study aimed to assess the genotoxicity, cytotoxicity, and bioactivity of four CSCs by comparing the newly introduced materials Bio MTA+ and MTA Cem with previously studied materials, Biodentine and NeoMTA. Methods Genotoxicity was evaluated using the micronucleus (MN) assay in human peripheral blood lymphocyte cells, measuring MN frequency and nuclear division index (NDI). Cytotoxicity was assessed in human dental pulp stem cells through the Water-Soluble Tetrazolium Salt-1 (WST-1) colorimetric assay. Bioactivity was determined by ELISA, measuring the levels of angiogenic and odontogenic markers (BMP-2, FGF-2, VEGF, and ALP). Statistical analyses included ANOVA, Dunnet and Sidak tests, and Wald chi-square test. (p

Abstract Background Nance–Horan syndrome (NHS; MIM 302,350) is an extremely rare X-linked dominant disease characterized by ocular and dental anomalies, intellectual disability, and facial dysmorphic features. Case presentation We report on five affected males and three carrier females from three unrelated NHS families. In Family 1, index (P1) showing bilateral cataracts, iris heterochromia, microcornea, mild intellectual disability, and dental findings including Hutchinson incisors, supernumerary teeth, bud-shaped molars received clinical diagnosis of NHS and targeted NHS gene sequencing revealed a novel pathogenic variant, c.2416 C > T; p.(Gln806*). In Family 2, index (P2) presenting with global developmental delay, microphthalmia, cataracts, and ventricular septal defect underwent SNP array testing and a novel deletion encompassing 22 genes including the NHS gene was detected. In Family 3, two half-brothers (P3 and P4) and maternal uncle (P5) had congenital cataracts and mild to moderate intellectual deficiency. P3 also had autistic and psychobehavioral features. Dental findings included notched incisors, bud-shaped permanent molars, and supernumerary molars. Duo-WES analysis on half-brothers showed a hemizygous novel deletion, c.1867delC; p.(Gln623ArgfsTer26). Conclusions Dental professionals can be the first-line specialists involved in the diagnosis of NHS due to its distinct dental findings. Our findings broaden the spectrum of genetic etiopathogenesis associated with NHS and aim to raise awareness among dental professionals.

Context: Knowledge of the microbial composition of abscessed primary tooth is limited. Aim: The aim was to investigate the presence of 10 oral bacterial species in samples from abscessed primary tooth root canals using microarray technology and to determine their association with clinical findings. Subjects and Methods: The samples were collected from root canals of 20 primary molars with acute primer infection. The bacterial composition of the samples was semi-quantitatively defined using a microarray system (ParoCheck®). Clinical parameters included the presence of spontaneous pain, mobility, percussion sensitivity and swelling. Statistical Analysis: Data were statistically analyzed by Student' t-test, Fisher's exact Chi-square test, Freeman–Halton–Fisher's exact test, and Spearman's rho correlation analysis. Results: All the tested species were detected in the samples. Fusobacterium nucleatum was the most frequent bacterium (100%), followed by Parvimonas micra (65%), Provetella intermedia (45%), and Treponema denticola (45%). According to paired bacterial combinations, F. nucleatum was significantly positively correlated with P. intermedia and P. micra (P < 0.05). T. denticola was significantly positively correlated with Porphyromonas gingivalis, Tannerella forsythia, Campylobacter rectus, and P. micra, while it was negatively correlated with Eikenella corrodens (P < 0.05). No statistically significant relationships were found between the presence of any bacteria and clinical findings. Conclusion: Microarray technology used in this study has demonstrated the presence of various bacteria with varying proportions in the root canals of abscessed primary teeth. The results regarding the high rate of certain bacterial combinations suggest the enhanced pathogenicity due to additive or synergistic effects of these microbial combinations.

Background: Talon cusp is a rare dental anomaly characterized by a cusp-like projection, often including the palatal surface of the affected tooth. The aim of the present study was to investigate the prevalence and characteristics of talon cusps in a group of Turkish children. Materials and Methods: The study population consisted of 14,400 subjects who attended the clinics of the Department of Pediatric Dentistry at the Istanbul University, Istanbul, Turkey . Subjects ranged in age from 1 to 14 years with a mean age of 10.5 ± 2.55 years. Talon cusps were mainly categorized by visual examination according to the classification of Hattab et al. The distribution and frequency of talon cusps were calculated with respect to dentition type, tooth type, talon type, the affected surface, associated dental anomalies, and clinical complications. Statistical analysis included descriptive statistics, frequencies, and crosstabs with Chi-square analysis. Results: Talon cusps were detected in 49 subjects (26 males and 23 females) of 14,400 (0.34%). A total of 108 teeth showed talon cusps. Distribution of talon cusps according to gender showed no statistically significant differences. The incidence of talon cusps was found to be greater in maxillary lateral incisors (53.7%) than central incisors (29.62%). Regarding the type of talon cusp, 47.22% of teeth showed a Type III talon cusp, whereas 30.55% of teeth demonstrated a Type II talon and 22.22% of teeth demonstrated a Type I talon cusp. Nine patients (18.36%) with talon cups also exhibited other developmental dental anomalies. Clinical complications associated with talon cusps were detected as caries formation and occlusal interference. Conclusion: This is the most comprehensive study of the prevalence of talon cusps in Turkish population using the largest sample size to date. Also, taurodontism associated with a talon cusp has been reported here for the 1 st time. Clinical complications associated with talon cusps need more investigations.

Introduction: Er:YAG laser is one of the most preferred laser types used in preparation of dental hard tissues. Since lased dentin surfaces have significantly different characteristics when compared to bur-prepared surfaces, adhesion performance of adhesive systems may differ, too. Microleakage is an important determinant in assessing the success of restorative materials and cavity preparation methods. Aim: The aim of this study was to assess the microleakage of three different adhesive systems in Er:YAG laser and bur prepared cavities. Materials and Methods: Cavities prepared either with Er:YAG laser or diamond bur were randomly assigned to eight groups (n=10): Group 1: Laser preparation+Clearfil Tri-S (C3S) Bond; Group 2: Laser preparation+Adper SE Plus (SE) Bond; Group 3: Laser preparation+ laser etching+ Adper Single Bond 2 (SB2); Group 4: Laser preparation+ acid etching+ SB2; Group 5: Laser preparation+SB2 (no etching); Group 6: Bur+acid etching+SB2; Group 7: Bur+C3S; Group 8: Bur+SE. The groups prepared conventionally (Groups 6-8) served as control groups. The teeth/ restoration interfaces were assessed for dye penetration by a stereomicroscope and an image analysis program. The data was analysed by Kruskal Wallis and Mann-Whitney U tests. Results: The highest microleakage was observed in the gingival interface of Group 5 and the lowest microleakage was seen in the occlusal interface of Group 3 and Group 6. When the effects of laser and bur preparation methods on occlusal and gingival microleakage levels were compared, the statistically significant difference was found between the SE Bond-applied groups (Groups 2 and 8). SE Bond has demonstrated significantly less microleakage in Er:YAG laser prepared cavities than bur prepared cavities. Conclusion: Interaction pattern of the adhesive systems with the lased substrate can differ from those with the conventional ones and particular characteristics of the adhesive systems have a strong influence on the success of the resin–dentin bond.

The principles of management of mandibular fractures differ in children when compared to adults and depend on the specific age-related status of the growing mandible and the developing dentition. This paper presents a case report with a complex facial trauma affecting the mandibular body and condyle region and dentoalveolar complex. Clinical examination revealed soft tissue injuries, limited mouth opening, lateral deviation of the mandible, an avulsed incisor, a subluxated incisor, and a fractured crown. CBCT examination revealed a nondisplaced fracture and an oblique greenstick fracture of the mandibular body and unilateral fracture of the condyle. Closed reduction technique was chosen to manage fractures of the mandible. Favorable healing outcomes on multiple fractures of the mandible throughout the 6-year follow-up period proved the success of the conservative treatment. This case report is important since it presents a variety of pathological sequelae to trauma within one case.

Aim:Turner syndrome (TS) is a genetic disorder caused by a numerical or structural aberration of the X chromosome, which is associated with a female phenotype. Concerning oral status, several studies have revealed that girls with TS have dental anomalies and periodontal problems. The aim of this study was to evaluate the effects of oral bacteria on caries prevalence and periodontal status in pediatric patients with TS.Materials and Methods:Twenty TS patients and 17 healthy girls were examined for cariological and periodontal status. The levels of mutans streptococci (MS), lactobacilli (LB), yeast and 10 different periodontal bacteria were determined by using culture and microarray techniques in children’s stimulated saliva samples.Results:There was no difference in salivary flow rate and buffering capacity, decayed-missing-filled teeth, MS, LB, or yeast levels between the groups. Plaque index and gingival index levels were significantly higher in the Turner group and dft was significantly higher in the control group (p

DOI: 10.26650/eor.2018.45227Purpose The aim of this study was to investigate the prevalence and characteristics of ectopically erupting first permanent molars (FPMs) in children attending the clinics of the Pediatric Dentistry Department at İstanbul University. Materials and methods This retrospective study was performed using panoramic radiographs of 7,649 patients (3,506 females and 4,143 males) aged from 5 to 11 years. The age and gender of the subjects, the number and location of the ectopic molars, bilateral versus unilateral occurrence, the degree of resorption of the roots of the primary molars, and other associated dental anomalies were assessed. Ectopic eruption was categorized according to a grading system based on the resorption rates of the primary molars. Results Of the 7,649 reviewed subjects, 203 (118 males and 85 females) were diagnosed with ectopic eruption of the FPMs, resulting in a frequency of 2.65%. The mean age of the subjects with ectopic FPMs was 6.82±1.25 (range: 5–11) years. Of the 273 ectopic FPMs, 157 (57.5%) were detected in the maxilla and 116 (42.5%) in the mandible. Severe and very severe degrees of ectopic eruption were found to be more common in the maxilla than in the mandible, whereas a moderate degree of ectopic eruption was more prevalent in the mandible (p=0.251). Conclusion To our knowledge, this is the first study in a Turkish population reporting the prevalence of ectopic eruption of FPMs. Although the difference between the right and the left sides was not significant, the severity of ectopic eruption was different between the maxilla and the mandible.

Purpose: The aim of the present study was to investigate the prevalence of dental anomalies in a Turkish population according to the gender and age. Materials and Methods: A retrospective study was performed using panoramic radiographs of 2025 patients (885 males and 1140 females) ranging in age from 9 to 35 (mean age 25.61±10.04) years attending Department of Oral Radiology, University of Istanbul, Faculty of Dentistry. These patients were examined to determine the presence of developmental dental anomalies involving hypodontia, hyperdontia, microdontia, taurodontism and other root anomalies. The incidence of these anomalies were assessed according to the gender and age. Results: Among the 2025 subjects, a total of 96 individuals (42 males and 54 females) showed at least one of the selected dental anomalies (4.74%). Tooth agenesis was the most common dental abnormality (1.77%) followed by taurodontism (1.18%), hyperdontia (0.79%), microdontia (0.54%) and root anomalies (0.44%), respectively. Conclusion: Tooth agenesis is the most common developmental dental anomaly in the studied Turkish population followed by taurodontism.