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4 results on '"Yebrail Gómez, Sergio"'

1. Apoptosis por el Virus de la Fiebre Amarilla investigada en células de mamífero crecidas directamente sobre láminas de vidrio

Author

Yebrail Gómez, Sergio, Valdivieso Q., Wilfredo, Yebrail Gómez, Sergio, and Valdivieso Q., Wilfredo

Abstract

Introduction: Programmed cell death referred to as apoptosis is a frequent event in animal cells infected by viruses. There are many procedures to investigate apoptosis in vitro, experiments requiring subculture cells growing in flask of 25 – 75 cm2 wich to result in prolonged time to get results. Objective: In this study was investigated apoptosis in Vero cells infected with yellow fever virus directly growing on glass slices used in immunofluorescent tests. Results: After 72 hours of virus infection, 93,2% of infected cells (in contrast with 2,6% of not infected cells) showed condensation of chromatin, using stain Hoescht 33342. In the same cells, loss of selective permeability was evidenced by stained with propidium iodide. Conclusion: This results show that, when we use a rapid culture cell system, it is possible to detect apoptosis during yellow fever virus replication in vitro., Introducción: La muerte celular programada o apoptosis es un evento frecuente en células infectadas por virus. Existen varios procedimientos para investigar apoptosis en experimentos in vitro, para los cuales se requiere subcultivar células que crecen en frascos de 25 o 75 cm2, lo que resulta en prolongación del tiempo para obtener el resultado. Objetivo: En este estudio se investigó apoptosis en células Vero infectadas con el virus de la fiebre amarilla y crecidas directamente sobre láminas de vidrio usadas en pruebas de inmunofluorescencia. Resultados: Se detectó condensación de cromatina usando el colorante Hoescht 33342 en 93,2% de células infectadas versus 2,6% de no infectadas, en las 72 horas siguientes a la infección (P< 0.01). En las células infectadas se evidenció pérdida de la permeabilidad selectiva de la membrana por coloración con yoduro de propidio. Conclusiones: Estos resultados indican que usando un sistema rápido de cultivo de células puede evaluarse apoptosis durante la replicación in vitro del virus de la fiebre amarilla.

Published
2008

2. Identificación de marcadores para riesgo cardiovascular en menores de 15 años de Floridablanca, Santander.

Author

Trejos, Juanita, Yebrail Gómez, Sergio, Angarita, Andrea Milena, Andrea Calderón, Jena, Alejandra Romero, Mayra, and Valdivieso, Wilfredo

Abstract

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2017

3. Potencial inhibitorio de moléculas aisladas de la planta Calea prunifolia sobre microorganismos de interés en salud humana.

Author

Yebrail Gómez, Sergio, Trejos, Juanita, Carlos Castaño, Jhon, and Gómez, Milton

Abstract

Copyright of Biomédica: Revista del Instituto Nacional de Salud is the property of Instituto Nacional de Salud of Colombia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2017

4. [Evaluation of PANBIO rapid immunochromatographic cassette for dengue diagnosis in a Colombian endemic area].

Author

Martínez-Vega RA, Díaz-Quijano FA, Coronel-Ruiz C, Yebrail Gómez S, and Villar-Centeno LA

Subjects
Acute Disease, Adolescent, Adult, Antibodies, Immobilized immunology, Antigens, Viral immunology, Child, Colombia epidemiology, Convalescence, Cross-Sectional Studies, Dengue blood, Dengue epidemiology, Early Diagnosis, Female, Fever blood, Fever diagnosis, Humans, Immunoglobulin G blood, Immunoglobulin M blood, Male, Middle Aged, Reproducibility of Results, Sensitivity and Specificity, Young Adult, Antibodies, Viral blood, Dengue diagnosis, Dengue Virus immunology, Endemic Diseases, Reagent Kits, Diagnostic, Serologic Tests
Abstract

Introduction: Traditional tests for dengue diagnosis are not useful during the first days of disease onset, because they require time and complex techniques or because they have low sensitivity. Objective. The reliability and precision was determined for the rapid test in cassette (PANBIO) for dengue diagnosis in acute and convalescent serum samples., Materials and Methods: The immunochromatographic test was evaluated in samples from patients older than 5 years with acute febrile syndrome. The patients came from the metropolitan area of Bucaramanga during April and August of 2003. Acute and convalescent serum came from 67 dengue cases, diagnosed by viral isolation or IgM paired tests (modified MAC-ELISA), and from 33 patients with acute febrile syndrome (not dengue) that served as controls., Results: Reliability was good (kappa=0.84). In the convalescent serum, the test showed sensitivity of 76.1%, specificity of 75.8%, positive predictive value of 86.4% and negative predictive value of 61.0%, positive likelihood ratio 3.14 and negative likelihood ratio 0.32. In the acute phase, the values were 52.2%, 84.8%, 87.5%, 46.7%, 3.45 and 0.56, respectively., Conclusions: Rapid immunochromatographic test for dengue diagnosis shows good reproducibility and specificity, with an acceptable sensitivity. Therefore, this rapid test is recommended as diagnostic tool in endemic areas.

Published
2009

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