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446 results on '"Yazawa, S."'

1. Multifaceted Analysis of Interstitial Pneumonias With Autoimmune Features: A Prospective Multicenter Cohort Study

Author

Enomoto, N., primary, Yazawa, S., additional, Toyoshima, M., additional, Kono, M., additional, Hashimoto, D., additional, Imokawa, S., additional, Fujii, M., additional, Akamatsu, T., additional, Koshimizu, N., additional, Yokomura, K., additional, Matsuda, H., additional, Mori, K., additional, Masuda, M., additional, Tabata, K., additional, Sumikawa, H., additional, and Suda, T., additional

Published
2023
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2. Development and characterization of a novel anti-fucosylated antigen monoclonal antibody YB-2 and its usefulness in the immunohistochemical diagnosis of colorectal cancer.

Author

Yazawa, S, Akamatsu, S, Tachikawa, T, Naito, H, Nakamura, J, Asao, T, Nagamachi, Y, Nakajima, T, Shin, S, and Chia, D

Subjects
Humans, Colorectal Neoplasms, Antibodies, Monoclonal, Antigens, Neoplasm, Immunoenzyme Techniques, Enzyme-Linked Immunosorbent Assay, Survival Analysis, Antibody Specificity, ANTI-FUCOSYLATED ANTIGEN ANTIBODY, TUMOR-ASSOCIATED ANTIGEN, COLORECTAL CANCER, IMMUNOHISTOCHEMICAL DIAGNOSIS, Antibodies, Monoclonal, Antigens, Neoplasm, Oncology & Carcinogenesis
Abstract

A novel monoclonal antibody, YB-2 was obtained after immunization of mice with fucosylated antigens isolated from human saliva. The antibody was demonstrated to react with Y (Fuc alpha 1-->2Gal-beta 1-->4[Fuc alpha 1-->3]GlcNAc beta),Leb (Fuc alpha 1-->2Gal beta 1-->3[Fuc alpha 1-->4]GlcNAc beta) and H type 2 (Fuc alpha 1-->2Gal-beta 1-->4GlcNAc beta) antigens, but not with H type 1 (Fuc alpha 1-->2Gal beta 1-->3GlcNAc beta), Lea (Gal beta 1-->3[Fuc alpha 1-->4]GlcNAc beta), X (Gal beta 1-->4[Fuc alpha 1-->3]GlcNAc beta) or with non-fucosylated antigens. Inhibition assays of YB-2 antibody with such reactive antigens showed that YB-2 antibody preferentially reacted with Y antigen. Formalin-fixed and paraffin-embedded sections prepared from normal and malignant colorectal tissues were examined immunohistochemically with YB-2. The positive rates of staining with YB-2 antibody were 88.6% in malignant and 12.0% in normal tissues. The expression of fucosylated antigens detected by YB-2 antibody seemed to be correlated with survival among patients with primary colorectal cancer. Therefore, YB-2 antibody could be useful as an immunochemical tool for diagnosis and evaluation of the prognosis of colorectal cancer.

Published
1993

14. Experimental Dual Infection of Specific Pathogen‐Free Pigs with Porcine Reproductive and Respiratory Syndrome Virus and Pseudorabies Virus

Author

Shibata, I., Yazawa, S., Ono, M., and Okuda, Y.

Subjects
Pseudorabies, Swine, viruses, animal diseases, Porcine Reproductive and Respiratory Syndrome, virus diseases, Original Articles, biochemical phenomena, metabolism, and nutrition, respiratory system, Antibodies, Viral, Herpesvirus 1, Suid, Specific Pathogen-Free Organisms, Animals, Porcine respiratory and reproductive syndrome virus, Lung, Administration, Intranasal
Abstract

Summary Twenty 6‐week‐old specific pathogen‐free pigs were divided into four groups. On day 0 of the experiment, PRRSV–PRV (n = 6) and PRRSV (n = 4) groups were intranasally inoculated with porcine reproductive and respiratory syndrome virus (PRRSV) (105.6 TCID50). On day 7, the PRRSV–PRV and PRV (n = 6) groups were intranasally inoculated with pseudorabies virus (PRV) (103.6 TCID50). Control pigs (n = 4) were kept as uninoculated negative controls. Half of the pigs in each group were euthanized and necropsied on day 14 or 21. Clinical signs such as depression and anorexia were observed in the PRRSV–PRV and PRV groups after inoculation with PRV. Although febrile response was observed after virus inoculations, the duration of that response was prolonged in the PRRSV–PRV group compared with the other groups. The lungs in the PRRSV–PRV group failed to collapse and were mottled or diffusely tan and red, whereas the lungs of the pigs in the other groups were grossly normal. Histopathologically, interstitial pneumonia was present in all PRRSV‐inoculated pigs, but the pneumonic lesions were more severe in the PRRSV–PRV group. Mean PRRSV titres of tonsil and lung in the PRRSV–PRV group were significantly (P

Published
2003

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