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1. Contents, Vol. 3, 1980

Author

Jacques Chanard, Anton Szymanowicz, Kazuaki Yamada, Sarwan S. Kang, Eileen F. Smith, Gerard M. Turino, J. Rakotoarivony, Kenji Iesato, P.L. Oe, E. Maxa, Nishio Honda, J.M. Foidart, Jose R. Manaligod, G. D’Amico, A. Bellini, L.A.M. Stolte, P. Bardos, J.A. Velosa, Hans Jørgen G. Gundersen, Barbara A. McKenna, Farhad Khalil-Manesh, Wesley Fox, L. van Delden, M. Sternberg, G.J. Fleuren, W.A. Day, A.R. McGiven, L.H. Noel, L.O. Simpson, Tito Cavallo, Philippe Birembaut, Jean-Pierre Brunois, Dick Heinegård, Friedrich C. Luft, Thomas W. Huang, Paul D. Benya, Billy G. Hudson, S.-L. Ou, Ruth Østerby, Cristina Kenney, Eric Sanders, R.J. Winand, Raymond C. Duhamel, Arnold Pollak, J.H. Veerkamp, Kunio Okuda, K. Hempel, Edward C. Carlson, J. Yudkin, J.M. Suc, Rytter Nørgaard, J.R. Rüttner, Wilhelm Kriz, Sarah A. Taylor, Michael F. Bryson, Jared J. Grantham, Harro Buss, H.E. Abboud, J. Goldman, T. Heck, R.G. Spiro, G. Sperk, Peter Schneider, Godfrey Heathcote, J.C. Orfila, O.T. Uttendorfsky, Gareth J. Thomas, James L. Borke, Harold C. Slavkin, S.V. Shah, I. Molenaar, R. Habib, Paul Jacques Borel, W. Schurer, C.F. Lange, M. Levy, Rajinder P. Nayyar, Kelvin T. Hughes, D. Droz, E.C.M. Ooms, L.A.H. Monnens, G. Rauscher, Jörgen Wieslander, C. Dubois, N.W. Levin, H.U. Lange, G. Goffinet, Teruo Mori, Kjartan Seyer-Hansen, Michael E. Grant, K.H. Winterhalter, F. Dumler, Will W. Minuth, Earl P. Benditt, B.F. Odermatt, Masafumi Wakashin, Frederick I. Volini, Günter Hollweg, Richard D. Spall, P.R. Macdonald, Olivier Toupance, C. Dechenne, A.P. Evan, J.P.M. Langeveld, Eiich Matsuo, G. Lubec, Cecil A. Krakower, Barry S. Oemar, H. Takamiya, Rufino C. Pabico, Elias Meezan, S. Batsford, J. Leibowitch, Gerald A. Coles, P. Graaff, G. Simbruner, Gert Lubec, W. Romen, C. Naizot, Yasumasa Takaya, A. Pollak, Bonnie Anderson Bray, Shiro Ueda, G. Colasanti, A.P. Sahu, Ines Mandl, F.C. Luft, Malcolm Davies, Bernard J. Partner, P. Mahieu, A. Vogt, T.P. Dousa, Yoko Wakashin, J. Moran, Andrew P. Evan, P. Cortes, P.J. Hoedemaeker, Tadashi Ofuji, M. Spiess, J.B. Foidart, Sadia Muhammed, Per Gygren, Yoshio Mori, K.K. Venkatachalam, Mistumasa Nagase, Zensuke Ota, Izumi Takei, Y.S. Pirard, Ole Gøtzsche, B. Nabarra, J.P. Muh, E. Ratzenhofer, H. Coradello, Anne E. Jackson, Anna G. Brownell, Hirofumi Makino, O. Förster, P. Freychet, J.S. Hunt, M.C. Gubler, P.R. Mahieu, B.H. Spargo, Ralph J. Butkowski, E. Meezan, B. Trüeb, Klaus Brendel, T. Oite, and Robert G. Price

Subjects
Nephrology, General Medicine, Cardiology and Cardiovascular Medicine
Published
1980

2. Contents, Vol. 4, 1981

Author

Tadashi Ofuji, Alan Terlinsky, Guido O. Perez, Harry G. Preuss, Kurt H. Albertine, Klaus Thomsen, Akira Miyoshi, Patricia J. O'Morchoe, Yasumasa Takaya, Murray Epstein, Charles Horton, Ole Vendelin Olesen, Hirofumi Makino, Raul Rodelas, Barbara Rietberg, Zensuke Ota, Yohkazu Matsushima, H. Jarosz, Munekazu Gemba, Charles C.C. O’ Morchoe, and Michael Gelfand

Subjects
Nephrology, General Medicine, Cardiology and Cardiovascular Medicine
Published
1981

3. Molecular sieve in rat tubular basement membrane as revealed by negative staining

Author

Yasumasa Takaya, Hirofumi Makino, Akira Miyoshi, Tadashi Ofuji, and Zensuke Ota

Subjects
Kidney Glomerulus, Ultrafiltration, In Vitro Techniques, urologic and male genital diseases, Molecular sieve, Basement Membrane, law.invention, Sonication, law, Albumins, medicine, Methods, Animals, Tubular basement membrane, Renal tubular basement membrane, Basement membrane, Staining and Labeling, Chemistry, General Medicine, Anatomy, Negative stain, Rats, Molecular Weight, Microscopy, Electron, Membrane, medicine.anatomical_structure, Kidney Tubules, Nephrology, Ultrastructure, Biophysics, Electron microscope, Cardiology and Cardiovascular Medicine
Abstract

Rat tubuli were isolated both by the method of Krisko and by a modified method of Cook and Pickering; renal tubular basement membrane (TBM) was isolated by sonic disruption and by the method of Carlson. Using electron microscopy after negative staining, TBM of rat kidney isolated under these different conditions was shown to be a fine meshwork. Strands of the meshwork were interwoven, apparently enclosing pores to make up whole basement membrane. These findings are compared with our previous observation that glomerular and alveolar basement membranes were made up of a similarly fine meshwork.

Published
1981

4. Molecular sieve in renal glomerular and tubular basement membranes as revealed by electron microscopy

Author

Yasumasa Takaya, Hirofumi Makino, Zensuke Ota, and Tadashi Ofuji

Subjects
Kidney Glomerulus, urologic and male genital diseases, Molecular sieve, Basement Membrane, law.invention, Sonication, law, medicine, Animals, Chromatography, Staining and Labeling, Chemistry, Glomerular basement membrane, Histological Techniques, Rats, Inbred Strains, General Medicine, Rats, Microscopy, Electron, medicine.anatomical_structure, Basement (geology), Membrane, Kidney Tubules, Nephrology, Biophysics, Cattle, Electron microscope, Cardiology and Cardiovascular Medicine
Abstract

Bovine glomerular basement membrane (GBM) was isolated and purified according to a modification of Spiro’s method. Rat and bovine tubular basement membranes (TBM) were isolated and purified by sonic disruption or by the method of Carlson et al. Electron microscopic studies on the ultrastructure of GBM and TBM were performed after negative staining with 1% phosphotungstic acid solution, pH 7.3. When negatively stained, GBM and TBM were seen as fragments varying in size. The surface of the membranes showed a characteristic felt-like or spongy appearance. At higher magnification, GBM and TBM showed a fine meshwork composed of strands and pores which three-dimensionally resembled a crystal lattice. Pores were fairly uniform in size and shape. They were round, oval or polygonal in shape. Some of the pores were elongated to form short straight or bent channels. Strands were also uniform in diameter and surrounded a pore or channel. For an average of 50 pores, the long dimension was 3.1 ± 0.6 nm and the short dimension 2.5 ± 0.3 nm in bovine GBM, 3.8 ± 1.2 and 2.5 + 0.7 nm in bovine TBM, and 4.9 ± 1.5 and 2.8 ± 0.6 nm in rat TBM, respectively. The strand was 1.8 ± 0.3 nm in diameter in bovine GBM, 2.5 ± 0.6 nm in bovine TBM and 3.7 ± 0.7 nm in rat TBM for an average of 50 strands. The diameters of the pores were less than or close to the short axis of an albumin molecule. It was concluded that renal GBM and TBM were molecular sieves composed of pores and strands.

Published
1980

5. Subject Index, Vol. 4, 1981

Author

Alan Terlinsky, Akira Miyoshi, Harry G. Preuss, Barbara Rietberg, Zensuke Ota, Charles Horton, Charles C.C. O’ Morchoe, Michael Gelfand, Yasumasa Takaya, Kurt H. Albertine, Tadashi Ofuji, Guido O. Perez, Hirofumi Makino, Raul Rodelas, Ole Vendelin Olesen, Munekazu Gemba, Klaus Thomsen, Patricia J. O'Morchoe, Yohkazu Matsushima, H. Jarosz, and Murray Epstein

Subjects
Index (economics), Nephrology, Statistics, Subject (documents), General Medicine, Cardiology and Cardiovascular Medicine, Mathematics
Published
1981

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