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Tumor mutational burden (TMB) is gaining attention as a biomarker for responses to immune checkpoint inhibitors in cancer patients. In this study, we evaluated the status of TMB in primary and liver metastatic lesions in patients with colorectal cancer (CRC). In addition, the status of TMB in primary and liver metastatic lesions was inferred by radiogenomics on the basis of computed tomography (CT) images. The study population included 24 CRC patients with liver metastases. DNA was extracted from primary and liver metastatic lesions obtained from the patients and TMB values were evaluated by next‐generation sequencing. The TMB value was considered high when it equaled to or exceeded 10/100 Mb. Radiogenomic analysis of TMB was performed by machine learning using CT images and the construction of prediction models. In 7 out of 24 patients (29.2%), the TMB status differed between the primary and liver metastatic lesions. Radiogenomic analysis was performed to predict whether TMB status was high or low. The maximum values for the area under the receiver operating characteristic curve were 0.732 and 0.812 for primary CRC and CRC with liver metastasis, respectively. The sensitivity, specificity, and accuracy of the constructed models for TMB status discordance were 0.857, 0.600, and 0.682, respectively. Our results suggested that accurate inference of the TMB status is possible using radiogenomics. Therefore, radiogenomics could facilitate the diagnosis, treatment, and prognosis of patients with CRC in the clinical setting., This study evaluated the status of tumor mutation burden (TMB) in primary and liver metastatic lesions in patients with colorectal cancer using radiogenomics. In 7 out of 24 patients (29.2%), the TMB status differed between the primary and liver metastatic lesion. Radiogenomic analysis was used to accurately predict whether TMB status was high or low.

Since particle beam distribution is vulnerable to change in bowel gas because of its low density, we developed a deep neural network (DNN) for bowel gas segmentation on X-ray images. We used 6688 image datasets from 209 cases as training data and 102 image datasets from 51 cases as test data. For the training data, we prepared three types of digitally reconstructed radiographic (DRR) images (all-density, bone and gas) by projecting the treatment planning CT image data. However, the real X-ray images acquired in the treatment room showed low contrast that interfered with manual delineation of bowel gas. Therefore, we used synthetic X-ray images converted from DRR images in addition to real X-ray images. We evaluated DNN segmentation accuracy for the synthetic X-ray images using Intersection over Union (IoU), recall, precision, and the Dice coefficient, which measured 0.708 ± 0.208, 0.832 ± 0.170, 0.799 ± 0.191, and 0.807 ± 0.178, respectively. The evaluation metrics for the real X-images were less accurate than those for the synthetic X-ray images (0.408 ± 0237, 0.685 ± 0.326, 0.490 ± 0272, and 0.534 ± 0.271, respectively). Our DNN appears useful in increasing treatment accuracy in particle beam therapy.

To ensure the safety of railroad operations, it is important to monitor and forecast track geometry irregularities. A higher safety requires forecasting with higher spatiotemporal frequencies, which in turn requires capturing spatial correlations. Additionally, track geometry irregularities are influenced by multiple exogenous factors. In this study, a method is proposed to forecast one type of track geometry irregularity, vertical alignment, by incorporating spatial and exogenous factor calculations. The proposed method embeds exogenous factors and captures spatiotemporal correlations using a convolutional long short-term memory. The proposed method is also experimentally compared with other methods in terms of the forecasting performance. Additionally, an ablation study on exogenous factors is conducted to examine their individual contributions to the forecasting performance. The results reveal that spatial calculations and maintenance record data improve the forecasting of vertical alignment., 21 pages, 7 figures

The selection of genes that are important for obtaining gene expression data is challenging. Here, we developed a deep learning-based feature selection method suitable for gene selection. Our novel deep learning model includes an additional feature-selection layer. After model training, the units in this layer with high weights correspond to the genes that worked effectively in the processing of the networks. Cancer tissue samples and adjacent normal pancreatic tissue samples were collected from 13 patients with pancreatic ductal adenocarcinoma during surgery and subsequently frozen. After processing, gene expression data were extracted from the specimens using RNA sequencing. Task 1 for the model training was to discriminate between cancerous and normal pancreatic tissue in six patients. Task 2 was to discriminate between patients with pancreatic cancer (n = 13) who survived for more than one year after surgery. The most frequently selected genes were ACACB, ADAMTS6, NCAM1, and CADPS in Task 1, and CD1D, PLA2G16, DACH1, and SOWAHA in Task 2. According to The Cancer Genome Atlas dataset, these genes are all prognostic factors for pancreatic cancer. Thus, the feasibility of using our deep learning-based method for the selection of genes associated with pancreatic cancer development and prognosis was confirmed.

PURPOSE Computed tomography (CT)-based attenuation correction (CTAC) in single-photon emission computed tomography (SPECT) is highly accurate, but it requires hybrid SPECT/CT instruments and additional radiation exposure. To obtain attenuation correction (AC) without the need for additional CT images, a deep learning method was used to generate pseudo-CT images has previously been reported, but it is limited because of cross-modality transformation, resulting in misalignment and modality-specific artifacts. This study aimed to develop a deep learning-based approach using non-attenuation-corrected (NAC) images and CTAC-based images for training to yield AC images in brain-perfusion SPECT. This study also investigated whether the proposed approach is superior to conventional Chang's AC (ChangAC). METHODS In total, 236 patients who underwent brain-perfusion SPECT were randomly divided into two groups: the training group (189 patients; 80%) and the test group (47 patients; 20%). Two models were constructed using Autoencoder (AutoencoderAC) and U-Net (U-NetAC), respectively. ChangAC, AutoencoderAC, and U-NetAC approaches were compared with CTAC using qualitative analysis (visual evaluation) and quantitative analysis (normalized mean squared error [NMSE] and the percentage error in each brain region). Statistical analyses were performed using the Wilcoxon signed-rank sum test and Bland-Altman analysis. RESULTS U-NetAC had the highest visual evaluation score. The NMSE results for the U-NetAC were the lowest, followed by AutoencoderAC and ChangAC (P

Radiogenomics has attracted attention for predicting the molecular biological characteristics of tumors from clinical images, which are originally a collection of numerical values, such as computed tomography (CT) scans. A prediction model using genetic information is constructed using thousands of image features extracted and calculated from these numerical values. In the present study, RNA sequencing of pancreatic ductal adenocarcinoma (PDAC) tissues from 12 patients was performed to identify genes useful in evaluating clinical pathology, and 107 PDAC samples were immunostained to verify the obtained findings. In addition, radiogenomics analysis of gene expression was performed by machine learning using CT images and constructed prediction models. Bioinformatics analysis of RNA sequencing data identified integrin αV (ITGAV) as being important for clinicopathological factors, such as metastasis and prognosis, and the results of sequencing and immunostaining demonstrated a significant correlation (r=0.625, P=0.039). Notably, the ITGAV high‑expression group was associated with a significantly worse prognosis (P=0.005) and recurrence rate (P=0.003) compared with the low‑expression group. The ITGAV prediction model showed some detectability (AUC=0.697), and the predicted ITGAV high‑expression group was also associated with a worse prognosis (P=0.048). In conclusion, radiogenomics predicted the expression of ITGAV in pancreatic cancer, as well as the prognosis.

Background Radiogenomics is an emerging field that integrates “Radiomics” and “Genomics”. In the current study, we aimed to predict the genetic information of pancreatic tumours in a simple, inexpensive, and non-invasive manner, using cancer imaging analysis and radiogenomics. We focused on p53 mutations, which are highly implicated in pancreatic ductal adenocarcinoma (PDAC), and PD-L1, a biomarker for immune checkpoint inhibitor-based therapies. Methods Overall, 107 patients diagnosed with PDAC were retrospectively examined. The relationship between p53 mutations as well as PD-L1 abnormal expression and clinicopathological factors was investigated using immunohistochemistry. Imaging features (IFs) were extracted from CT scans and were used to create prediction models of p53 and PD-L1 status. Results We found that p53 and PD-L1 are significant independent prognostic factors (P = 0.008, 0.013, respectively). The area under the curve for p53 and PD-L1 predictive models was 0.795 and 0.683, respectively. Radiogenomics-predicted p53 mutations were significantly associated with poor prognosis (P = 0.015), whereas the predicted abnormal expression of PD-L1 was not significant (P = 0.096). Conclusions Radiogenomics could predict p53 mutations and in turn the prognosis of PDAC patients. Hence, prediction of genetic information using radiogenomic analysis may aid in the development of precision medicine.

Study design Retrospective analysis of magnetic resonance imaging (MRI). Objective The aim of this study was to evaluate the performance of our convolutional neural network (CNN) in differentiating between spinal schwannoma and meningioma on MRI. We compared the performance of the CNN and that of two expert radiologists. Summary of background data Preoperative discrimination between spinal schwannomas and meningiomas is crucial because different surgical procedures are required for their treatment. A deep-learning approach based on CNNs is gaining interest in the medical imaging field. Methods We retrospectively reviewed data from patients with spinal schwannoma and meningioma who had undergone MRI and tumor resection. There were 50 patients with schwannoma and 34 patients with meningioma. Sagittal T2-weighted magnetic resonance imaging (T2WI) and sagittal contrast-enhanced T1-weighted magnetic resonance imaging (T1WI) were used for the CNN training and validation. The deep learning framework Tensorflow was used to construct the CNN architecture. To evaluate the performance of the CNN, we plotted the receiver-operating characteristic (ROC) curve and calculated the area under the curve (AUC). We calculated and compared the sensitivity, specificity, and accuracy of the diagnosis by the CNN and two board-certified radiologists. Results . The AUC of ROC curves of the CNN based on T2WI and contrast-enhanced T1WI were 0.876 and 0.870, respectively. The sensitivity of the CNN based on T2WI was 78%; 100% for radiologist 1; and 95% for radiologist 2. The specificity was 82%, 26%, and 42%, respectively. The accuracy was 80%, 69%, and 73%, respectively. By contrast, the sensitivity of the CNN based on contrast-enhanced T1WI was 85%; 100% for radiologist 1; and 96% for radiologist 2. The specificity was 75%, 56, and 58%, respectively. The accuracy was 81%, 82%, and 81%, respectively. Conclusion We have successfully differentiated spinal schwannomas and meningiomas using the CNN with high diagnostic accuracy comparable to that of experienced radiologists. Level of evidence 4.

In massively multiplayer online role-playing games (MMORPGs), each race holds some attributes and skills. Each skill contains several abilities such as physical damage and hit rate. All those attributes and abilities are functions of the character's level, which are called Ability-Increasing Functions (AIFs). A well-balanced MMORPG is characterized by having a set of well-balanced AIFs. In this paper, we propose a coevolutionary design method, including integration with the modified probabilistic incremental program evolution (PIPE) and the cooperative coevolutionary algorithm (CCEA), to solve the balance problem of MMORPGs. Moreover, we construct a simplest turn-based game model and perform a series of experiments based on it. The results indicate that the proposed method is able to obtain a set of well-balanced AIFs more efficiently, compared with the simple genetic algorithm (SGA), the simulated annealing algorithm (SAA) and the hybrid discrete particle swarm optimization (HDPSO) algorithm. The results also show that the performance of PIPE has been significantly improved through the modification works.

Form of sea wave is greatly affected by not just flow and depth of sea water, but also wind blowing on the sea surface. Therefore, measurement and analysis of sea wave motion is of assistance for control and operational safety of boats and ships. Generally, oceanic information is gauged by acoustic and electric wave. But these methods have not provided enough spatial and temporal resolution, and are completely out of touch with the on-site needs. Thus, the LED liar for sea wave measurement has been developed. The dynamic analysis of sea wave image measured by the LED lidar was conducted and the relationship with wind speed was evaluated. In this report, we first present the specifications and measurement methodologies of the LED lidar. Then we describe the actual measurements of sea wave with shallow angle by using this lidar and the results of their analysis.

In the case of on-line action role-playing game, the combat strategies can be divided into three distinct classes, Strategy of Motion(SM), Strategy of Attacking Occasion (SAO) and Strategy of Using Skill (SUS). In this paper, we analyze such strategies of a basic game model in which the combat is modeled by the discrete competitive Markov decision process. By introducing the chase model and the combat assistant technology, we identify the optimal SM and the optimal SAO, successfully. Also, we propose an evolutionary framework, including integration with competitive coevolution and cooperative coevolution, to search the optimal SUS pair which is regarded as the Nash equilibrium point of the strategy space. Moreover, some experiments are made to demonstrate that the proposed framework has the ability to find the optimal SUS pair. Furthermore, from the results, it is shown that using cooperative coevolutionary algorithm is much more efficient than using simple evolutionary algorithm.

In on-line role-playing games (RPG), each race holds some attributes and skills. Each skill contains several abilities such as physical damage, hit rate, etc. Parts of the attributes and all the abilities are a function of the character’s level, which are called Ability-Increasing Functions (AIFs). A well-balanced on-line RPG is characterized by having a set of well-balanced AIFs. In this paper, we propose an evolutionary design method, including integration with an improved Probabilistic Incremental Program Evolution (PIPE) and a Cooperative Coevolutionary Algorithm (CCEA), for on-line RPGs to maintain the game balance. Moreover, we construct a simplest turn-based game model and perform a series of experiments based on it. The results indicate that the proposed method is able to obtain a set of well-balanced AIFs efficiently. They also show that in this case the CCEA outperforms the simple genetic algorithm, and that the capability of PIPE has been significantly improved through the improvement work.

A discrimination method is proposed in which a class region is taken as a set of quasi convex hulls. A subclass method approximates a class region by a set of hyper-rectangles, while the proposed method uses a set of quasi convex hulls. The experimental results show the effectiveness of the proposed method.

Cooperative Coevolutionary Algorithm (CCEA) has been widely applied to solve large scale and complex problems, which result in significant speedups over traditional genetic algorithm. However, CCEA do not search for the optimal solutions but the compromised solutions because of its nature of cooperation. So in the case that the object problem has infinite Nash equilibria, CCEA is expected to converge into suboptimal solution even employing the shared archive. In this paper, we propose a Nash equilibria preservation strategy which enables CCEA to jump out of the suboptimal solution and to keep searching without falling into the same suboptimal solutions found so far. The experiment results show that the capability of the archive-shared CCEA has been improved by using the proposed strategy, especially in dealing with problem having infinite Nash equilibria.

We address the balance problem of on-line Action Role-playing Games(ARPGs) by using an evolutionary architecture which includes intergration with an improved Probabilistic Incremental Program Evolution(PIPE) and Cooperative Coevolutionary Algorithm(CCEA). We construct a game model of the basic on-line ARPG and use it to obtain the training data for a statistical model named Linear Logistic Regression Model(LLRM) by monte carlo method, then we calculate fitness value of the individual based on the prediction of the LLRM. Moreover, some experiments are made to demonstrate the evolutionary architecture is able to find a set of well-balanced Abilityincreasing Functions(AIFs), which determine how to increase the ability value with level, for the target game more efficiently and logically.

Bengalese Finch (shape Lonchura striata var. domestica) sings more sophisticated songs than other birds and the grammar of their songs has been found to be described with probabilistic finite state automaton (PFA). In the present paper, we propose a multilayer neural network model that succeeds in reproducing the qualitatively similar symbol sequence by taking into account the memory process. The use of the present method is illustrated for the songs of Bengalese Finch with particular emphasis on issues of input delay that is necessary to obtain the correct grammar. It is found that the grammar obtained from the simulated symbol sequence using the PFA agrees well with the real one.

Supervised data structures in high dimensional feature spaces are displayed as graphs. The structure is analyzed by normal mixture distributions. The nodes of the graph correspond the mean vectors of the mixture distributions, and the location is carried out by Sammon’s nonlinear mapping. The thickness of the edges expresses the separability between the component distributions, which is determined by Kullback-Leibler divergence. From experimental results, it was confirmed that the proposed method can illustrate in which regions and to what extent it is difficult to classify samples correctly. Such visual information can be utilized for the improvement of the feature sets.