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122 results on '"Yasuda, Masahiko"'

2. Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping

Author

Gee, Peter, Lung, Mandy S. Y., Okuzaki, Yuya, Sasakawa, Noriko, Iguchi, Takahiro, Makita, Yukimasa, Hozumi, Hiroyuki, Miura, Yasutomo, Yang, Lucy F., Iwasaki, Mio, Wang, Xiou H., Waller, Matthew A., Shirai, Nanako, Abe, Yasuko O., Fujita, Yoko, Watanabe, Kei, Kagita, Akihiro, Iwabuchi, Kumiko A., Yasuda, Masahiko, Xu, Huaigeng, Noda, Takeshi, Komano, Jun, Sakurai, Hidetoshi, Inukai, Naoto, and Hotta, Akitsu

Published
2020
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5. SGX523 causes renal toxicity through aldehyde oxidase-mediated less-soluble metabolite formation in chimeric mice with humanized livers.

Author

Uehara, Shotaro, Yasuda, Masahiko, Higuchi, Yuichiro, Yoneda, Nao, Kawai, Kenji, Suzuki, Masami, Yamazaki, Hiroshi, and Suemizu, Hiroshi

Subjects
*NEPHROTOXICOLOGY, *ORAL drug administration, *BLOOD urea nitrogen, *KIDNEY tubules, *PROTEIN-tyrosine kinase inhibitors, *LIVER, *PROXIMAL kidney tubules
Abstract

SGX523 is a c-Met tyrosine kinase inhibitor that failed in clinical trials because of renal toxicity caused by crystal deposits in renal tubules. SGX523 is metabolized by aldehyde oxidase (AOX) in a species-dependent manner to the considerably less soluble 2-quinolinone-SGX523, which is likely involved in the clinically observed obstructive nephropathy. This study investigated the metabolism and renal toxicity of SGX523 in chimeric mice with humanized livers (humanized-liver mice). The 2-quinolinone-SGX523 formation activity was higher in humanized-liver mouse and human hepatocytes than in mouse hepatocytes. Additionally, this activity in the liver cytosolic fraction from humanized-liver mice was inhibited by the AOX inhibitors raloxifene and hydralazine. After oral SGX523 administration, higher maximum concentrations, larger areas under the plasma concentration versus time curves, and higher urinary concentrations of 2-quinolinone-SGX523 were observed in humanized-liver mice than in non-humanized mice. Serum creatinine and blood urea nitrogen levels were elevated in humanized-liver mice following repeated oral SGX523 administration. The accumulation of amorphous material in the tubules and infiltration of inflammatory cells around tubules were observed in the kidneys of humanized-liver mice after repeated oral SGX523 administration. These findings demonstrate that humanized-liver mice are useful for understanding the metabolism and toxicity of SGX523. [Display omitted] • Human AOX1 metabolizes SGX523 into 2-quinolinone SGX523 in humanized-liver mice. • The less soluble 2-quinolinone SGX523 may be involved in SGX523-induced nephropathy. • Humanized-liver mice can help predict metabolism-dependent drug toxicities. [ABSTRACT FROM AUTHOR]

Published
2023
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7. Characterization of hiPSC-Derived Muscle Progenitors Reveals Distinctive Markers for Myogenic Cell Purification Toward Cell Therapy

Author

20447965, 50578002, 80528745, Nalbandian, Minas, Zhao, Mingming, Sasaki-Honda, Mitsuru, Jonouchi, Tatsuya, Lucena-Cacace, Antonio, Mizusawa, Takuma, Yasuda, Masahiko, Yoshida, Yoshinori, Hotta, Akitsu, Sakurai, Hidetoshi, 20447965, 50578002, 80528745, Nalbandian, Minas, Zhao, Mingming, Sasaki-Honda, Mitsuru, Jonouchi, Tatsuya, Lucena-Cacace, Antonio, Mizusawa, Takuma, Yasuda, Masahiko, Yoshida, Yoshinori, Hotta, Akitsu, and Sakurai, Hidetoshi

Abstract

The transplantation of muscle progenitor cells (MuPCs) differentiated from human induced pluripotent stem cells (hiPSCs) is a promising approach for treating skeletal muscle diseases such as Duchenne muscular dystrophy (DMD). However, proper purification of the MuPCs before transplantation is essential for clinical application. Here, by using MYF5 hiPSC reporter lines, we identified two markers for myogenic cell purification: CDH13, which purified most of the myogenic cells, and FGFR4, which purified a subset of MuPCs. Cells purified with each of the markers showed high efficiency for regeneration after transplantation and contributed to the restoration of dystrophin expression in DMD-immunodeficient model mice. Moreover, we found that MYF5 regulates CDH13 expression by binding to the promoter regions. These findings suggest that FGFR4 and CDH13 are strong candidates for the purification of hiPSC-derived MuPCs for therapeutical application.

Published
2021

9. Pathogenesis of murine astrovirus in experimentally infected mice

Author

Morita, Hanako, primary, Yasuda, Masahiko, additional, Yamamoto, Masafumi, additional, Tomiyama, Yurina, additional, Uchida, Ritsuki, additional, Ka, Yuyo, additional, Ogura, Tomoyuki, additional, Kawai, Kenji, additional, Suemizu, Hiroshi, additional, and Hayashimoto, Nobuhito, additional

Published
2021
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11. Extracellular nanovesicles for packaging of CRISPR-Cas9 protein and sgRNA to induce therapeutic exon skipping

Author

10722811, 00422410, 80528745, 50578002, Gee, Peter, Lung, Mandy S. Y., Okuzaki, Yuya, Sasakawa, Noriko, Iguchi, Takahiro, Makita, Yukimasa, Hozumi, Hiroyuki, Miura, Yasutomo, Yang, Lucy F., Iwasaki, Mio, Wang, Xiou H., Waller, Matthew A., Shirai, Nanako, Abe, Yasuko O., Fujita, Yoko, Watanabe, Kei, Kagita, Akihiro, Iwabuchi, Kumiko A., Yasuda, Masahiko, Xu, Huaigeng, Noda, Takeshi, Komano, Jun, Sakurai, Hidetoshi, Inukai, Naoto, Hotta, Akitsu, 10722811, 00422410, 80528745, 50578002, Gee, Peter, Lung, Mandy S. Y., Okuzaki, Yuya, Sasakawa, Noriko, Iguchi, Takahiro, Makita, Yukimasa, Hozumi, Hiroyuki, Miura, Yasutomo, Yang, Lucy F., Iwasaki, Mio, Wang, Xiou H., Waller, Matthew A., Shirai, Nanako, Abe, Yasuko O., Fujita, Yoko, Watanabe, Kei, Kagita, Akihiro, Iwabuchi, Kumiko A., Yasuda, Masahiko, Xu, Huaigeng, Noda, Takeshi, Komano, Jun, Sakurai, Hidetoshi, Inukai, Naoto, and Hotta, Akitsu

Abstract

Prolonged expression of the CRISPR-Cas9 nuclease and gRNA from viral vectors may cause off-target mutagenesis and immunogenicity. Thus, a transient delivery system is needed for therapeutic genome editing applications. Here, we develop an extracellular nanovesicle-based ribonucleoprotein delivery system named NanoMEDIC by utilizing two distinct homing mechanisms. Chemical induced dimerization recruits Cas9 protein into extracellular nanovesicles, and then a viral RNA packaging signal and two self-cleaving riboswitches tether and release sgRNA into nanovesicles. We demonstrate efficient genome editing in various hard-to-transfect cell types, including human induced pluripotent stem (iPS) cells, neurons, and myoblasts. NanoMEDIC also achieves over 90% exon skipping efficiencies in skeletal muscle cells derived from Duchenne muscular dystrophy (DMD) patient iPS cells. Finally, single intramuscular injection of NanoMEDIC induces permanent genomic exon skipping in a luciferase reporter mouse and in mdx mice, indicating its utility for in vivo genome editing therapy of DMD and beyond.

Published
2020

14. Pentatrichomonas hominis in laboratory-bred common marmosets

Author

Inoue, Takashi, Hayashimoto, Nobuhito, Yasuda, Masahiko, Sasaki, Erika, and Itoh, Toshio

Subjects
Male, marmoset, Base Sequence, Original, Genes, Protozoan, Monkey Diseases, Callithrix, Genes, rRNA, Pentatrichomonas hominis, Intestines, Trichomonadida, protozoa, Feces, Japan, Animals, Laboratory, parasitic diseases, Trichomonas, Animals, Female, Protozoan Infections, Animal
Abstract

Trichomonadid protozoa have been found in the intestinal tracts of common marmosets (Callithrix jacchus). However, there is little information available on species identification and the pathogenicity of these trichomonads. In this study, we conducted a fecal survey of a common marmoset colony maintained as laboratory animals in Japan and identified the trichomonad species. Screening using a fecal smear examination revealed that 66% (58/88) of the marmosets had trichomonadid trophozoites in their feces. The trichomonads were found in both normal feces (31/49, 63%) and diarrhea (27/39, 69%), with no significant difference in frequency. The protozoa were identified as Pentatrichomonas hominis using morphological characters and the 100% identity of the nucleotide sequence of the partial 18S rRNA gene (297 bp). The intraspecific genetic variability between P. hominis from the marmosets in this study and P. hominis from other reported mammal hosts was ≤1% in the nucleotide sequence, including the internal transcribed spacer (ITS)-1, 5.8S rRNA gene, and ITS-2 (293 bp). P. hominis inhabits the large intestine of various mammalian hosts, including primates, and is considered nonpathogenic. These results suggest that P. hominis is transmitted among marmosets and other mammals but is not a primary cause of bowel disease in marmosets.

Published
2015

16. Microbiological survey of mice (Mus musculus) purchased from commercial pet shops in Kanagawa and Tokyo, Japan

Author

HAYASHIMOTO, Nobuhito, MORITA, Hanako, ISHIDA, Tomoko, UCHIDA, Ritsuki, TANAKA, Mai, OZAWA, Midori, YASUDA, Masahiko, and ITOH, Toshio

Subjects
Infectious disease, Risk Management, Original, Pets, Communicable Diseases, Pet shop, Rodent Diseases, Mice, Zoonosis, Japan, Animals, Laboratory, Zoonoses, Communicable Disease Control, Animals, Tokyo
Abstract

Information regarding the prevalence of infectious agents in mice in pet shops in Japan is scarce. This information is particularly useful for minimizing the risk of potential transmission of infections to laboratory mice. Therefore, we surveyed infectious agents in mice from pet shops in Kanagawa and Tokyo, Japan. The survey was conducted in 28 mice from 5 pet shops to screen for 47 items (17 viruses, 22 bacteria and fungi, 10 parasites) using culture tests, serology, PCR, and microscopy. The most common viral agent detected was murine norovirus (17 mice; 60.7%), followed by Theiler's murine encephalomyelitis virus (13 mice; 46.4%), and mouse hepatitis virus (12 mice; 42.8%). The most common agent amongst the bacteria and fungi was Pasteurella pneumotropica (10 mice; 35.7%), followed by Helicobacter ganmani and Pneumocystis murina (8 mice; 28.5%, for both). Tritrichomonas muris was the most common parasite (19 mice; 67.8%), followed by Spironucleus muris (13 mice; 46.4%), Aspiculuris tetraptera, and Syphacia obvelata (8 mice each; 28.5%). Remarkably, a zoonotic agent, Hymenolepis nana, was found in 7 mice (25%). Given these results, we suggest that the workers in laboratory animal facilities should recognize again the potential risks of mice outside of the laboratory animal facilities as an infectious source, and avoid keeping mice as pets or as feed for carnivorous reptiles as much as possible for risk management.

Published
2014

18. Background data on NOD/Shi-scid IL-2Rγnull mice (NOG mice)

Author

Kasahara, Kenichiro, primary, Fukunaga, Yachiyo, additional, Igura, Saori, additional, Andoh, Rie, additional, Saito, Tsubasa, additional, Suzuki, Isamu, additional, Kanemitsu, Hiroyuki, additional, Suzuki, Daisuke, additional, Goto, Ken, additional, Nakamura, Daichi, additional, Mochizuki, Masahiro, additional, Yasuda, Masahiko, additional, Inoue, Ryo, additional, Tamura, Kazutoshi, additional, and Nagatani, Mariko, additional

Published
2017
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