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Many Nanoparticles (NPs) were reported to significantly enhance the production of biogas from the anaerobic digestion of organic wastes. Herein, two types of NPs (Ni-Ferrite and Ni-Co-Ferrite) were synthesized using the co-precipitation method and characterized using X-ray diffraction (XRD), and high-resolution transmission electron microscopy (HRTEM) with energy dispersive X-ray spectroscopy (EDS) techniques. Three different concentrations (20, 70 and 130 mg/l) of each type were added to separate biogas reactors to study their effect on the biogas production compared to blank reactor. A pressure-based biogas reactor was designed and manufactured specifically to test the effect of the NPs on the anaerobic digestion process. The pressure of the daily produced biogas was monitored using a pressure transducer and the volume of the biogas was calculated at standard conditions of pressure (1.013 bar) and temperature (15 °C). The results showed an increase in the biogas production of 6.6%, 5.9% and 32.9% upon the use of Ni-Co-Ferrite NPS at concentrations of 20, 70, and 130 mg/l compared to blank reactor, respectively. In addition, the Ni-Ferrite NPs achieved biogas enhancements of 30.8%, 28.5%, and 17.9% at concentrations of 20, 70 and 130 mg/l, respectively.