1. [Enzymatic Synthesis of Acidic β-Lactams (Review)].
- Author
-
Sklyarenko AV, EI'darov MA, Kurochkina VB, and Yarotskii SV
- Subjects
- Acids, Anti-Bacterial Agents biosynthesis, Bacterial Proteins genetics, Biocatalysis, Cephalosporins biosynthesis, Enzymes, Immobilized chemistry, Enzymes, Immobilized genetics, Escherichia coli enzymology, Escherichia coli genetics, Gene Expression, Hydrogen-Ion Concentration, Multienzyme Complexes genetics, Penicillin Amidase genetics, Penicillins biosynthesis, Recombinant Proteins chemistry, Recombinant Proteins genetics, Solvents chemistry, Temperature, Anti-Bacterial Agents chemical synthesis, Bacterial Proteins chemistry, Cephalosporins chemical synthesis, Multienzyme Complexes chemistry, Penicillin Amidase chemistry, Penicillins chemical synthesis
- Abstract
The currently known methods of enzymatic β-lactam synthesis, as well as the enzymes and heterogeneous biocatalysts used for this purpose, are presented, and the published reports on advances in the field of enzymatic synthesis of selected antibiotics belonging to the groups of acidic penicillins and acidic cephalosporins are summarized in the present review. The key conditions and parameters of biocatalytic processes, such as the biocatalyst form, concentration of the precursor compounds, solvent type, pH, temperature, etc. are analyzed and compared, and guidelines for further optimization of β-lactam synthesis are given. The present review may be of use for a wide range of readers, as well as to enzymology and biotechnology experts.
- Published
- 2015