11 results on '"Yari R"'
Search Results
2. Production and Simple Purification of Recombinant Human Granulocyte Colony-Stimulating Factor using the Inteintag in Escherichia Coli
- Author
-
Peymanfar P, Roghanian R, Ghaedi K, H, Sayed, and Yari R
- Subjects
Recombinant ,Granulocyte Colony Stimulating Factor ,Escherichia Coli ,Recombinant Proteins - Abstract
Background: Granulocyte colony-stimulating factor (G-CSF) is a cytokine which have many functions such as stimulating of hematopoiesis, proliferation and differentiation of granulocyte progenitor cells and production of bone marrow neutrophilic granulocyte colonies. Nowadays, the usage of human recombinant G-CSF (rh G-CSF) is for curing of chemotherapy- radiotherapy-induced neutropenia, and also in patients with bone marrow transplantation. The aim of this study was to produce a soluble type of G-CSF in E.coli and purification in new way. In this study, we achieved active G-CSF using the intein fusion system. Therefore, two copies of optimized Intein - GCSF genewas cloned into the pET22b vector to generate pET22b-G-CSF-intein2 (C-terminal fusion vector) and pET22b intein1-G-CSF (N-terminal fusion vector). The hG-CSF sequence with two different intein sequence from pTWIN1 vector are synthesized and then inserted into a pET22 expression vector under the control of T7 promoter and cloned in E. coli strain BL21 (DE3).The fused proteins formed misfolded proteins as inclusion bodies (IBs). The IBs were solved with urea solution and afterward proteins were saved for size-exclusion chromatography. Then , CBD-intein–GCSF was loaded onto chitin beads column equilibrated with 10mM Tris buffer, 500mM NaCl, pH 8.5, and was eluted from the column by incubation at 25°C under pH 6.5 for 16h based on intein C-terminal self-cleavage. SDS-Page, western blot, dot blot, size exclusion chromatography and in vivo assay of protein demonstrated that the bioactivity of recombinant GCSF was compared with standard GCSF. Results: After culturing and induction of recombinant E. coli with IPTG, we obtained a good expression of the hG-CSF, as determined by SDS-PAGE and confirmed by dot blotting and western blotting. Approximately 50% of the expressed G-CSF was soluble after the IPTG induction and temperature reduction. Up to 1.2 mg of G-CSF was achieved from a 1 L culture, and nearly75% of the GCSF-Intein2 was cleared by pH shift. Intein1-GCSF was not cleaved due to the inhibition of cleavage by the N-terminal amino acid of GCSF. The biological activity assay, in vivo, showed a higher biological effect than standard reference hG-CSF. Conclusion: The immunological and biological analyses showed that this protocol can be useful to develop bioproducts. In conclusion, the combination of different methods presented here permitted an simple and cost-effective protocol for rhG-CSF production.
- Published
- 2016
3. The Relation between Age, Gender and Apoptosis Regulator FasL Gene Expression in Multiple Sclerosis Patients
- Author
-
Faraz, A., primary, Yari, R., primary, Taheri, M., additional, Omrani, M., additional, Saberi, M., additional, Mazdeh, M., additional, and Sayad, A., additional
- Published
- 2016
- Full Text
- View/download PDF
4. Antibacterial activity of Tribulus terrestris methanol extract against clinical isolates of Escherichia coli
- Author
-
Batoei Sara, Mahboubi Mohaddese, and Yari Reza
- Subjects
tribulus terrestris ,methanol extract ,synergy ,gentamicin ,saponins ,Plant culture ,SB1-1110 - Abstract
Introduction:Tribulus terrestris L. is traditionally used for treatment of urinary tract infections. Escherichia coli, as the most prominent agent of urinary tract infections, can be sensitive to T. terrestris extract.
- Published
- 2016
- Full Text
- View/download PDF
5. Unforeseen plant phenotypic diversity in a dry and grazed world.
- Author
-
Gross N, Maestre FT, Liancourt P, Berdugo M, Martin R, Gozalo B, Ochoa V, Delgado-Baquerizo M, Maire V, Saiz H, Soliveres S, Valencia E, Eldridge DJ, Guirado E, Jabot F, Asensio S, Gaitán JJ, García-Gómez M, Martínez P, Martínez-Valderrama J, Mendoza BJ, Moreno-Jiménez E, Pescador DS, Plaza C, Pijuan IS, Abedi M, Ahumada RJ, Amghar F, Arroyo AI, Bahalkeh K, Bailey L, Ben Salem F, Blaum N, Boldgiv B, Bowker MA, Branquinho C, van den Brink L, Bu C, Canessa R, Castillo-Monroy ADP, Castro H, Castro P, Chibani R, Conceição AA, Darrouzet-Nardi A, Davila YC, Deák B, Donoso DA, Durán J, Espinosa C, Fajardo A, Farzam M, Ferrante D, Franzese J, Fraser L, Gonzalez S, Gusman-Montalvan E, Hernández-Hernández RM, Hölzel N, Huber-Sannwald E, Jadan O, Jeltsch F, Jentsch A, Ju M, Kaseke KF, Kindermann L, le Roux P, Linstädter A, Louw MA, Mabaso M, Maggs-Kölling G, Makhalanyane TP, Issa OM, Manzaneda AJ, Marais E, Margerie P, Hughes FM, Messeder JVS, Mora JP, Moreno G, Munson SM, Nunes A, Oliva G, Oñatibia GR, Peter G, Pueyo Y, Quiroga RE, Ramírez-Iglesias E, Reed SC, Rey PJ, Reyes Gómez VM, Rodríguez A, Rolo V, Rubalcaba JG, Ruppert JC, Sala O, Salah A, Sebei PJ, Stavi I, Stephens C, Teixido AL, Thomas AD, Throop HL, Tielbörger K, Travers S, Undrakhbold S, Val J, Valkó O, Velbert F, Wamiti W, Wang L, Wang D, Wardle GM, Wolff P, Yahdjian L, Yari R, Zaady E, Zeberio JM, Zhang Y, Zhou X, and Le Bagousse-Pinguet Y
- Subjects
- Animals, Climate Change, Geographic Mapping, Biodiversity, Desert Climate, Herbivory physiology, Livestock physiology, Phenotype, Plants chemistry, Plants classification
- Abstract
Earth harbours an extraordinary plant phenotypic diversity
1 that is at risk from ongoing global changes2,3 . However, it remains unknown how increasing aridity and livestock grazing pressure-two major drivers of global change4-6 -shape the trait covariation that underlies plant phenotypic diversity1,7 . Here we assessed how covariation among 20 chemical and morphological traits responds to aridity and grazing pressure within global drylands. Our analysis involved 133,769 trait measurements spanning 1,347 observations of 301 perennial plant species surveyed across 326 plots from 6 continents. Crossing an aridity threshold of approximately 0.7 (close to the transition between semi-arid and arid zones) led to an unexpected 88% increase in trait diversity. This threshold appeared in the presence of grazers, and moved toward lower aridity levels with increasing grazing pressure. Moreover, 57% of observed trait diversity occurred only in the most arid and grazed drylands, highlighting the phenotypic uniqueness of these extreme environments. Our work indicates that drylands act as a global reservoir of plant phenotypic diversity and challenge the pervasive view that harsh environmental conditions reduce plant trait diversity8-10 . They also highlight that many alternative strategies may enable plants to cope with increases in environmental stress induced by climate change and land-use intensification., (© 2024. The Author(s), under exclusive licence to Springer Nature Limited.)- Published
- 2024
- Full Text
- View/download PDF
6. Biosynthesized BiFe 2 O 4 @Ag nanoparticles mediated Scenedesmus obliquus induce apoptosis in AGS gastric cancer cell line.
- Author
-
Shamsi H, Yari R, and Salehzadeh A
- Subjects
- Humans, Cell Line, Tumor, Cell Survival drug effects, Antineoplastic Agents pharmacology, Antineoplastic Agents chemistry, HEK293 Cells, X-Ray Diffraction, Apoptosis drug effects, Stomach Neoplasms drug therapy, Stomach Neoplasms pathology, Stomach Neoplasms metabolism, Metal Nanoparticles chemistry, Scenedesmus drug effects, Silver chemistry, Silver pharmacology
- Abstract
The use of magnetic metal nanoparticles has been considered in cancer treatment studies. In this study, BiFe
2 O4 @Ag nanoparticles were synthesized biologically by Scenedesmus obliquus for the first time and their anticancer mechanism in a gastric cancer cell line was characterized. The physicochemical properties of the nanoparticles were evaluated by fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDS), scanning electron microscopy (SEM), transmission electron microscopy (TEM), dynamic Light Scattering (DLS), and zeta potential analyses. Cell viability and nuclear damage were investigated by the MTT and Hoechst staining assays, respectively. Flow cytometry analysis was performed to determine the frequency of the necrotic and apoptotic cells as well as cell cycle analysis of the nanoparticles-treated cells. Physicochemical characterization showed that the synthesized particles were spherical, without impurities, in a size range of 38-83 nm, with DLS size and zeta potential of 295.7 nm and -27.7 mV, respectively. BiFe2 O4 @Ag nanoparticles were considerably more toxic for the gastric cancer cells (AGS cell line) than HEK293 normal cells with IC50 of 67 and 117 µg/ml, respectively. Treatment of AGS cells with the nanoparticles led to a remarkable increase in the percentage of late apoptosis (38.5 folds) and cell necrosis (13.4 folds) and caused cell cycle arrest, mainly at the S phase. Also, nuclear fragmentation and apoptotic bodies were observed in the gastric cancer cells treated with the nanoparticles. This study represents BiFe2 O4 @Ag as a novel anticancer candidate against gastric cancer that can induce cell apoptosis through DNA damage and inhibition of cell cycle progression., (© 2024. The Author(s).)- Published
- 2024
- Full Text
- View/download PDF
7. Influence of Different Glucose Concentrations on the Expression of miR-29c-3p microRNA in Mesenchymal Stem Cells.
- Author
-
Mansournejad S, Mehrabi M, Yari R, and Saleh M
- Abstract
Background: miR-29c-3p manages a set of genes involved in regenerative medicine, and It seems that hyperglycemia in diabetic patients influences the power of stem cells to tissue regeneration the difficulties of diabetes by affecting the expression miR-29c-3p in mesenchymal stem cells. The study aims to analyze the effect of various glucose concentrations on the miR -29c-3p expression in mesenchymal stem cells. Materials and Methods: After receiving donated mesenchymal stem cells from Tarbiat Modares University, these cells were cultivated in a DMEM culture medium, including three different concentrations of glucose 250, 140, and 100 mg/dl. RNA was extracted from these cells after 72 hours, the Real-Time PCR technique assessed the expression of miR-29c-3p, and the results were analyzed by REST software. Results: miR-29c-3p expression in cells at concentrations of 140 and 250 mg/dL compared to typical situations (100 mg/dl) was significantly decreased (P˂0.05), which declined at a concentration of 250 mg/dl was more. Conclusion: Reduced miR-29c-3p expression in mesenchymal stem cells in chronic and mild diabetic situations demonstrated that diabetes might be one of the significant reasons for mesenchymal stem cells' reduced ability to repair tissue damage., Competing Interests: The authors declare that they have no competing interests., (Copyright © 2024 Tehran University of Medical Sciences.)
- Published
- 2024
- Full Text
- View/download PDF
8. Characterization and In Silico Analysis of The Structural Features of G-CSF Derived from Lysates of Escherichia coli.
- Author
-
Peymanfar SH, Roghanian R, Ghaedi K, Zarkesh-Esfahani SH, and Yari R
- Abstract
Objective: Granulocyte colony-stimulating factor (G-CSF) has a wide variety of functions including stimulation of hematopoiesis and proliferation of granulocyte progenitor cells. Recombinant human G-CSF (rh-G-CSF) is used for treatment of neutropenia in patients receiving chemotherapy. The mature bloodstream neutrophils express G-CSF receptor (G-CSFR), presenting a significant and specific mechanism for circulating G-CSF clearance. Computational studies are essential bioinformatics methods used for characterization of proteins with regard to their physicochemical properties and 3D configuration, as well as protein-ligand interactions for recombinant drugs. We formerly produced rh-G-CSF in E. coli and showed that the isolated protein had unacceptable biological activity in mice. In the present paper, we aimed to characterize the purified rh-G-CSF by analytical tests and developed an in vivo model by computational modelling of G-CSF., Materials and Methods: In this experimental study, we analyzed the purified G-CSF using the analytical experiments. Then, the crystalline structure was extracted from Protein Data Bank (PDB) and molecular dynamics (MD) simulation was performed using Gromacs 5.1 package under an Amber force field. The importance of amino acid contents of G-CSF, to bind the respective receptor was also detected; moreover, the effect of dithiothreitol (DTT) used in G-CSF purification was studied., Results: The results revealed that characteristics of the produced recombinant G-CSF were comparable with those of the standard G-CSF and the recombinant G-CSF with the residual amino acid was stable. Also, purification conditions (DTT and existence of extra cysteine) had a significant effect on the stability and functionality of the produced G-CSF., Conclusion: Experimental and in silico analyses provided good information regarding the function and characteristics of our recombinant G-CSF which could be useful for industrial researches., Competing Interests: There is no conflict of interest in this study., (Copyright© by Royan Institute. All rights reserved.)
- Published
- 2020
- Full Text
- View/download PDF
9. Synthesis of Nano-Paramagnetic Oleuropein to Induce KRAS Over-Expression: A New Mechanism to Inhibit AGS Cancer Cells.
- Author
-
Barzegar F, Zaefizadeh M, Yari R, and Salehzadeh A
- Subjects
- Cell Proliferation drug effects, Humans, Iridoid Glucosides, Iridoids pharmacology, Nanoparticles therapeutic use, Olea enzymology, Olea genetics, Proto-Oncogene Proteins p21(ras) blood, Spectrometry, X-Ray Emission methods, Adenocarcinoma prevention & control, Gene Expression drug effects, Iridoids therapeutic use, Proto-Oncogene Proteins p21(ras) biosynthesis, Stomach Neoplasms prevention & control
- Abstract
Background and objectives: Human gastric adenocarcinoma (AGS) is one of the most common malignant cancers worldwide. The present study aimed to transfer oleuropein into cancer cells using synthetic paramagnetic nanoparticles and study their effect on the AGS (ATCC
® CRL1739™) cell line. Materials and Methods: Paramagnetic nano-oleuropein was synthesized using four-stage co-precipitation by developing NH-connected bridges and was evaluated by EDS, SEM and FTIR methods. Different concentrations of magnetic oleuropein (0, 0.15, 0.45, 1.37, 4.12, 12.35, 37.04, 111.11, 333.33, 1000 µg/mL) were used to treat the AGS cell line in a completely randomized design using a statistical framework with three replicates. The relative expression rate of miR-200 and KRAS oncogenes was evaluated using real-time PCR. The inhibition rate of the AGS cells was assessed using the MTT test at 24, 48 and 72 h intervals. Results: The results showed that there was a significant difference between the inhibition rates of magnetic nano-oleuropein at IC50-24h (23.6 µg/mL), IC50-48h (15.2 µg/mL) and IC50-72h (9.2 µg/mL). Real-time PCR indicated that the relative expression of KRAS and miR-200 genes was highest at IC50 at these intervals. Conclusions: Magnetic nano-oleuropein can be subjected to objective testing and clinical evaluations as a natural antioxidant to prevent and treat gastric adenocarcinoma.- Published
- 2019
- Full Text
- View/download PDF
10. MicroRNAs and exosomes in depression: Potential diagnostic biomarkers.
- Author
-
Tavakolizadeh J, Roshanaei K, Salmaninejad A, Yari R, Nahand JS, Sarkarizi HK, Mousavi SM, Salarinia R, Rahmati M, Mousavi SF, Mokhtari R, and Mirzaei H
- Subjects
- Animals, Biomarkers metabolism, Depression therapy, Humans, Depression diagnosis, Depression metabolism, MicroRNAs metabolism
- Abstract
Depression is known as one of important psychiatric disorders which could be associated with disability among various populations. Diagnostic and statistical manual of mental disorders, 4th edition (DSM-IV) and international statistical classification of diseases and related health problems (ICD-10) could be used as subjective diagnostic schemes for identification of mental disorders such as depression. Utilization of subjective diagnostic schemes are associated with limitations. Hence, it seems that employing of new diagnosis platforms is required. Multiple lines of evidence indicated that measurement of several biomarkers could be useful for detection patients with depression. Among of various types of biomarkers, microRNAs (miRNAs) have been emerged as powerful tools for diagnosis patients with depression. MiRNAs are small non-coding RNAs which act as epigenetic regulators. It has been showed that these molecules have critical roles in pathogenesis of many diseases such as depression. These molecules exert their effects via targeting a variety of cellular and molecular pathways involved in initiation and progression of depression. Hence, miRNAs could be used as diagnostic and therapeutic biomarkers in patients with depression. Besides miRNAs, exosomes as nano- carriers could have been emerged as diagnostic biomarkers in several diseases such as depression. These vesicles are able to carry several cargos such as DNAs, proteins, mRNAs, and miRNAs to recipient cells. Here, we summarized several miRNAs involved in pathogenesis and response to treatment of depression which could be used as diagnostic biomarkers. Moreover, we highlighted exosomes as new diagnostic biomarkers for patients with depression., (© 2017 Wiley Periodicals, Inc.)
- Published
- 2018
- Full Text
- View/download PDF
11. Circulating microRNAs in Hepatocellular Carcinoma: Potential Diagnostic and Prognostic Biomarkers.
- Author
-
Mirzaei HR, Sahebkar A, Mohammadi M, Yari R, Salehi H, Jafari MH, Namdar A, Khabazian E, Jaafari MR, and Mirzaei H
- Subjects
- Animals, Carcinoma, Hepatocellular blood, Humans, Liver Neoplasms blood, Biomarkers, Tumor blood, Carcinoma, Hepatocellular diagnosis, Circulating MicroRNA blood, Liver Neoplasms diagnosis
- Abstract
Hepatocellular carcinoma (HCC) is one of the most common types of malignancies worldwide. There is little information on the mechanisms involved in the pathogenesis of this disease. Diagnosis of HCC at early stages would be crucial for increasing the survival of patients. Circulating miRNAs have emerged as one of the most attractive tools for an early diagnosis of cancers. Various studies have shown that there is an aberrant expression of miRNAs such as miR-25, miR-375, miR-206, miR-223, miR- 92a, miR-222, miR-1, let- 7f and miR-21 in HCC. Circulating and tissue miRNAs have also key roles in the pathogenesis of HCC by affecting several biologically important pathways such as p53, p21, PTEN, PI3K-AKT, c-Myc and STAT3. In this review, we summarize the current knowledge on the role of miRNAs in diagnosis, prognosis, and treatment of HCC.
- Published
- 2016
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.