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The Gullele Botanic Garden was established to preserve and safeguard indigenous, rare, endemic, endangered, and economically important plant species. The objective of this study was to identify and map the exotic, invasive, and potentially invasive plant species that are present in the garden’s various land use types, such as natural vegetation, plantations, roadsides and garden edges. The research involved laying plots at different distances in each land use type and collecting vegetation data with geo-location information. Sorensen’s similarity index was used to measure the floristic similarity between the sampled land use types. Data on species density and abundance were analyzed using the corresponding formula. The Shannon–Wiener diversity index and evenness were used to compute the diversity of the species in each land use type using R packages. ArcGIS version 10.5 was used to track the geographical distribution and map the exotic, invasive, and potentially invasive species that exist in all land use types of the garden. A total of 80 plant species belonging to 70 genera in 44 families were recorded in the garden. Asteraceae, Myrtaceae, and Fabaceae comprised the highest number of species. Acacia decurrens, Acacia melanoxylon, Cuscuta campestris, Galinsoga parviflora, Nerium oleander, and Cyathula uncinulata were the most prevalent invasive and potentially invasive species. The study found that the roadside and garden edge land use types had the most diverse exotic and invasive plants. The total density of exotic species was 2.36 plants/m2. The potential possibility of these plants in displacing the native plant species is quite high unless the introduction of exotic plant species is inspected and appropriate management strategies for invasive species are put in place.