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4 results on '"Yaojun Luo"'

2. mRNA m

Author

Youhua, Liu, Ying, Yang, Ruifan, Wu, Chun-Chun, Gao, Xing, Liao, Xiao, Han, Botao, Zeng, Chaoqun, Huang, Yaojun, Luo, Yuxi, Liu, Yushi, Chen, Wei, Chen, Jiaqi, Liu, Qin, Jiang, Yuanling, Zhao, Zhen, Bi, Guanqun, Guo, Yongxi, Yao, Yun, Xiang, Xiaojun, Zhang, Teresa G, Valencak, Yizhen, Wang, and Xinxia, Wang

Subjects
Adipogenesis, Swine, Animals, RNA-Binding Proteins, RNA, Messenger, Muscle Development, RNA Transport
Abstract

Although 5-methylcytosine (m

Published
2022

3. mRNA m5C controls adipogenesis by promoting CDKN1A mRNA export and translation

Author

Chaoqun Huang, Wei Chen, Botao Zeng, Guanqun Guo, Yaojun Luo, Yizhen Wang, Xinxia Wang, Yuxi Liu, Yushi Chen, Yuanling Zhao, Ruifan Wu, Youhua Liu, and Xing Liao

Subjects
Cyclin-Dependent Kinase Inhibitor p21, Messenger RNA, Adipogenesis, RNA-Binding Proteins, Translation (biology), Methyltransferases, Cell Biology, Biology, Methylation, environment and public health, RNA Transport, Cell biology, Mice, Gene Expression Regulation, 3T3-L1 Cells, Protein Biosynthesis, RNA modification, 5-Methylcytosine, Animals, RNA, Messenger, Epigenetics, human activities, Molecular Biology, Research Paper
Abstract

5-Methylcytosine (m5C) is a type of RNA modification that exists in tRNAs and rRNAs and was recently found in mRNA. Although mRNA m5C modification has been reported to regulate diverse biological process, its function in adipogenesis remains unknown. Here, we demonstrated that knockdown of NOL1/NOP2/Sun domain family member 2 (NSUN2), a m5C methyltransferase, increased lipid accumulation of 3T3-L1 preadipocytes through accelerating cell cycle progression during mitotic clonal expansion (MCE) at the early stage of adipogenesis. Mechanistically, we proved that NSUN2 directly targeted cyclin-dependent kinase inhibitor 1A (CDKN1A) mRNA, a key inhibitory regulator of cell cycle progression, and upregulated its protein expression in an m5C-dependent manner. Further study identified that CDKN1A was the target of Aly/REF export factor (ALYREF), a reader of m5C modified mRNA. Upon NSUN2 deficiency, the recognition of CDKN1A mRNA by ALYREF was suppressed, resulting in the decrease of CDKN1A mRNA shuttling from nucleus to cytoplasm. Thereby, the translation of CDKN1A was reduced, leading to the acceleration of cell cycle and the promotion of adipogenesis. Together, these findings unveiled an important function and mechanism of the m5C modification on adipogenesis by controlling cell cycle progression, providing a potential therapeutic target to prevent obesity.

Published
2021
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4. Extraction and HPLC Characterization of Chlorogenic Acid from Tobacco Residuals.

Author

Yuru Chen, Qiming Jimmy Yu, Xuemei Li, Yaojun Luo, and Hui Liu

Subjects
EXTRACTION (Chemistry), CHLOROGENIC acid, POLYPHENOLS, HIGH performance liquid chromatography, TOBACCO, PHYSICAL & theoretical chemistry
Abstract

Chlorogenic acid is a highly valuable natural polyphenol compound used in medicine and industries. Its current commercial sources are from plant extracts of Lonicera japonica Thunb and Eucommia ulmoides Oliver. These sources are limited and expensive. On the other hand, tobacco residuals contain chlorogenic acid and other natural polyphenol compounds. Large quantities of tobacco residuals are produced each year as waste materials from tobacco manufacturing, potentially providing an alternative commercial source of chlorogenic acid and other valuable compounds. In this paper, microwave and ultrasound extractions of chlorogenic acid with mixed solvent were studied. Total polyphenol concentrations in extract solutions obtained with different extraction methods were analyzed with the method of ferrous tartrate and UV-Vis spectrophotometry and compared. The extraction solutions were also characterized for polyphenol compositions with the method of HPLC. Experimental results indicated that high extract concentrations of chlorogenic acid were obtained with a mixed solvent of acetone and water (1:2 v/v). A total polyphenol concentration of up to 4.87 mg/ml and a chlorogenic acid concentration of up to 2.12 mg/ml were achieved. The application of microwave and ultrasound significantly increased the extract concentrations. The extraction time needed was also much reduced. HPLC analysis indicated that acetone water mixed solvent extraction achieved much higher relative concentrations of chlorogenic acid to other compounds in the extract solutions. These results indicted that fast and effective extraction of chlorogenic acid from tobacco residuals were achieved. [ABSTRACT FROM AUTHOR]

Published
2007
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