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Porcine growth hormone (pGH) is most important hormone which is involved in the growth and development of pig. However, a series of studies have indicated that neonatal pig is insensitive to pGH; the reason for this phenomenon is still not fully understood. In this work, we try to investigate this issue from the angle of intracellular signaling induced by pGH. In the present study, porcine hepatocytes from neonatal pig were used as a model, and confocal laser scanning microscopy (CLSM), Western blot, co-immunoprecipitation and colocalization assay were used to study pGH’s signaling properties in hepatocytes of neonatal pig and explore the possible mechanism(s) for why intracellular signaling is insensitive to pGH. The results indicated that Janus kinase 2 and signal transducers and activators of transcription 5/3/1 (JAK2-STATs) signaling are not activated. We further investigated the possible mechanism(s) by which JAK2-STATs’ signaling is not activated by pGH and growth hormone receptor (GHR) and found that the negative regulatory molecules of JAK2-STATs signaling may be associated with this phenomenon in the hepatocytes of neonatal pig. In addition, we also explored pGH’s biology in hepatocytes from neonatal pig, it can be found that pGH/GHR could translocate into the cell nucleus, which implies that pGH/GHR may exhibit physiological roles based on their nuclear localization. We found that pGH could not trigger intracellular signaling in the hepatocytes of neonatal pigs, but not young pigs, which provides an important explanation for why the growth of neonatal pig is GH independent.

The fall of the ore-rock will have an impact-tamping effect on the materials stored in a vertical orepass and affect the flow characteristics of the storage materials and the stress state of the orepass wall, which may cause the blockage of the orepass and the deformation and damage of the shaft wall. The research adopts a numerical simulation method forming a cylindrical orepass wall and creating the storage materials in the orepass with randomly distributed spheres and irregular polyhedrons, analyzes the change law of the impact force on the shaft wall and the material in the orepass, and the change law of the voidage of the storage materials under the conditions of different initial ore-rock falling rates. The results indicate that (1) the impact of falling ore-rock on the shaft wall is concentrated in some range below the material surface, and the impact of the shaft wall around the material surface is the most obvious; (2) the materials' voidage decreases with the increase of the ore-rock falling rate, and its variation is most apparent in part close to the material surface, but the overall voidage changes slightly; (3) the internal force chain system is stable after the accumulation of materials in the orepass is formed, the impact process of the ore-rock falling just affects the force chain with a length of fewer than four blocks and has no noticeable effect on the skeleton structure composed of longer force chains at the lower part in the orepass. [ABSTRACT FROM AUTHOR]

An abstract of the article "A relationship between endometrial microvascular density and VEGF expression during estrous cycle of sheep," by Ge Chen-Xia and colleagues is presented.

The article presents a study which investigates the dynamic changes of vascular endothelial growth factor (VEGF) in ovine's ovary by combining the computer graphic analytical technique and immunohistology.

The article examines and evaluates the hybrid vigor differential expressed gene GHR of crossbreed and cloned muscovy ducks in Beijing, China.