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With the development of industry and agriculture, a large amount of phosphorus is discharged into natural water bodies, leading to eutrophication in water bodies. Traditional chemical and biological methods cannot effectively remove low concentration of phosphate from water. Combining adsorbent with ultrafiltration membrane is expected to capture and adsorb low concentration of phosphate by cross-flow filtration. Dolomite modified biochar adsorbent(DO/BB) was loaded into polyvinylidene difluoride(PVDF) ultrafiltration membrane(PVDF-DO/BB). The microstructure, mechanical property and phosphate adsorption/separation performance of PVDF-DO/BB were investigated. The results showed that compared with pure PVDF membrane, the surface roughness of PVDF-DO/BB composite membrane increased and its hydrophilicity deteriorated. The tensile strength of PVDF-DO/BB was increased by about 23%, and the flexibility was also improved. When the initial phosphate concentration was 1.0 mg/L, it could be reduced to 0.16 mg/L after PVDF-DO/BB treatment, and the removal rate remained >75% after five adsorption-desorption cycles. Through the models fitting of adsorption kinetic and isotherm data, it was found that the adsorption process was mainly controlled by monolayer chemical reaction. The distribution of active sites was uniform. Combined with the XPS analysis of PVDF-DO/BB before and after phosphate adsorption, it was clarified that the adsorption mechanism mainly consisted of electrostatic attraction, inner sphere monodentate complex formation, inner sphere bidentate complex formation and hydroxyapatite precipitation. Moreover, PVDF-DO/BB could efficiently reject macromolecular pollutants, with the dual function of adsorption-ultrafiltration.

Abstract Currently, three carnivorous bat species, namely Ia io, Nyctalus lasiopterus, and Nyctalus aviator, are known to actively prey on seasonal migratory birds (hereinafter referred to as “avivorous bats”). However, the absence of reference genomes impedes a thorough comprehension of the molecular adaptations of avivorous bat species. Herein, we present the high-quality chromosome-scale reference genome of N. aviator based on PacBio subreads, DNBSEQ short-reads and Hi-C sequencing data. The genome assembly size of N. aviator is 1.77 Gb, with a scaffold N50 of 102 Mb, of which 99.8% assembly was anchored into 21 pseudo-chromosomes. After masking 635.1 Mb repetitive sequences, a total of 19,412 protein-coding genes were identified, of which 99.3% were functionally annotated. The genome assembly and gene prediction reached 96.1% and 96.1% completeness of Benchmarking Universal Single-Copy Orthologs (BUSCO), respectively. This chromosome-level reference genome of N. aviator fills a gap in the existing information on the genomes of carnivorous bats, especially avivorous ones, and will be valuable for mechanism of adaptations to dietary niche expansion in bat species.

Abstract Background Microglia is the major contributor of post-stroke neuroinflammation cascade and the crucial cellular target for the treatment of ischemic stroke. Currently, the endogenous mechanism underlying microglial activation following ischemic stroke remains elusive. Serglycin (SRGN) is a proteoglycan expressed in immune cells. Up to now, the role of SRGN on microglial activation and ischemic stroke is largely unexplored. Methods Srgn knockout (KO), Cd44-KO and wild-type (WT) mice were subjected to middle cerebral artery occlusion (MCAO) to mimic ischemic stroke. Exogenous SRGN supplementation was achieved by stereotactic injection of recombinant mouse SRGN (rSRGN). Cerebral infarction was measured by 2,3,5-triphenyltetrazolium chloride (TTC) staining. Neurological functions were evaluated by the modified neurological severity score (mNSS) and grip strength. Microglial activation was detected by Iba1 immunostaining, morphological analysis and cytokines’ production. Neuronal death was examined by MAP2 immunostaining and FJB staining. Results The expression of SRGN and its receptor CD44 was significantly elevated in the ischemic mouse brains, especially in microglia. In addition, lipopolysaccharide (LPS) induced SRGN upregulation in microglia in vitro. rSRGN worsened ischemic brain injury in mice and amplified post-stroke neuroinflammation, while gene knockout of Srgn exerted reverse impacts. rSRGN promoted microglial proinflammatory activation both in vivo and in vitro, whereas Srgn-deficiency alleviated microglia-mediated inflammatory response. Moreover, the genetic deletion of Cd44 partially rescued rSRGN-induced excessed neuroinflammation and ischemic brain injury in mice. Mechanistically, SRGN boosted the activation of NF-κB signal, and increased glycolysis in microglia. Conclusion SRGN acts as a novel therapeutic target in microglia-boosted proinflammatory response following ischemic stroke.

Summary: Background: Schizophrenia, a debilitating psychiatric disorder, displays considerable interindividual variation in clinical presentations. The ongoing debate revolves around whether this heterogeneity signifies a continuum of severity linked to a singular causative factor or a collection of distinct subtypes with unique origins. Within the realm of schizophrenia, the functional impairment of GluN2A, a subtype of the NMDA receptor, has been associated with an elevated risk. Despite GluN2A’s expression across various neuronal types throughout the brain, its specific contributions to schizophrenia and its involvement in particular cell types or brain regions remain unexplored. Methods: We generated age-specific, cell type-specific or brain region-specific conditional knockout mice targeting GluN2A and conducted a comprehensive analysis using tests measuring phenotypes relevant to schizophrenia. Findings: Through the induction of germline ablation of GluN2A, we observed the emergence of numerous schizophrenia-associated abnormalities in adult mice. Intriguingly, GluN2A knockout performed at different ages, in specific cell types and within distinct brain regions, we observed overlapping yet distinct schizophrenia-related phenotypes in mice. Interpretation: Our interpretation suggests that the dysfunction of GluN2A is sufficient to evoke heterogeneous manifestations associated with schizophrenia, indicating that GluN2A stands as a prominent risk factor and a potential therapeutic target for schizophrenia. Funding: This project received support from the Shanghai Municipal Science and Technology Major Project (Grant No. 2019SHZDZX02) awarded to Y.C. and the Natural Science Foundation of Shanghai (Grant No. 19ZR1468600 and 201409003800) awarded to G.Y.

Hoxb5 exhibits preferential expression in hematopoietic stem cells (HSCs) and uniquely marks the long-term HSCs (LT-HSCs). Previous studies have demonstrated the remarkable capability of Hoxb5 to alter cell fates when enforced expression in blood progenitors, such as B cell progenitors and multipotent progenitors. Additionally, Hoxb5 deficiency does not hinder the generation of LT-HSCs. However, the specific impact of Hoxb5 deletion on LT-HSCs has remained unexplored. To address this, we developed a conditional Hoxb5 knockout-reporter mouse model, wherein Hoxb5 was knock out by the Vav-cre recombinase, and the endogenous Hoxb5 promoter drove the expression of the blue fluorescent protein (BFP). Our findings revealed that the primary recipients, who transplanted with HSCs indicating Hoxb5 deficiency by the presence of BFP (BFP-positive HSCs), exhibited comparable levels of donor chimerism and lineage chimerism to recipients transplanted with HSCs that spontaneously did not express Hoxb5 and thus lacked BFP expression (BFP-negative HSCs). However, during the secondary transplantation, recipients receiving total bone marrow (BM) from the primary recipients with BFP-positive HSCs showed significantly higher levels of donor chimerism and more robust multi-lineage chimerism compared to those receiving total BM from the primary recipients with BFP-negative HSCs. Our results indicate that deleting Hoxb5 in LT-HSCs transiently influences their lineage differentiation bias without compromising their long-term self-renewal capacity. These findings highlight the primary role of Hoxb5 in regulating lineage commitment decisions in LT-HSCs, while emphasizing that its presence is not indispensable for the maintenance of long-term self-renewal capacity.

Abstract Background The niche breadth of an animal population comprises both within-individual and between-individual variation (individual specialization). Both components can be used to explain changes in population niche breadth, and this has been extensively investigated in dietary niche dimension studies. However, little is known about how changes in food resources or environmental factors across seasons affect changes in individual and population space use within the same population. Methods In this study, we used micro-GPS loggers to capture the space use of individuals and of a population of the great evening bat (Ia io) in summer and autumn. We used I. io as a model to investigate how individual spatial niche breadth and spatial individual specialization affect changes in population niche breadth (home range and core area sizes) across seasons. Additionally, we explored the drivers of individual spatial specialization. Results We found that the population home range and the core area of I. io did not increase in autumn when insect resources were reduced. Moreover, I. io showed different specialization strategies in the two seasons: higher spatial individual specialization in summer and lower individual specialization but broader individual niche breadth in autumn. This trade-off may maintain the dynamic stability of the population spatial niche breadth across seasons and facilitate the population response to changes in food resources and environmental factors. Conclusions Like diet, spatial niche breadth of a population also may be determined by a combination of individual niche breadth and individual specialization. Our work provides new insights into the evolution of niche breadth from the spatial dimension.

Summary: The nocturnal and cryptic nature of bats makes it difficult to determine their foraging behavior and predatory sensory cues. Here, we present a protocol for determining the behavioral strategies and sensory cues of bat predation on airborne prey. We describe steps for indoor foraging behavior simulation, dual-choice acoustic playback, and visual presentation experiments. This protocol was used to study the avivorous bat, Ia io, but it can be adapted for studying other aerial-hawking bats and even other taxa.For complete details on the use and execution of this protocol, please refer to Gong et al. (2022).1 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Compressed sensing magnetic resonance imaging (CS-MRI) has made great progress in speeding up MRI imaging. The existing non-local self-similarity (NSS) prior based CS-MRI models mainly take similar image patches as the processing objects, this patch-level non-local sparse representation method can not make full use of the self-similarity among pixels in the image, so it can not recover the weak edge information in the undersampled MRI image well and there will still be some artifacts. In this paper, a pixel-level non-local method based compressed sensing undersampled MRI image reconstruction method is introduced. First, zero filling is performed on the undersampled k-space data to obtain a full-size 2D signal, and IFFT is performed to obtain a preliminary reconstructed MRI image. Block-matching and row-matching are successively performed on the reconstructed image in turn to obtain similar pixel groups, so as to establish a better sparse representation under the non-local self-similarity (NSS) prior. The separable Haar transform is performed on similar pixel groups, and the hard threshold of the transform coefficients and Wiener filtering can effectively remove the artifacts introduced in the undersampled reconstructed MRI images. The proposed pixel-level non-local iterative thinning model based on compressed sensing theory can ensure the removal of artifacts and better restore the details in the image. The qualitative and quantitative results under different undersampling modes and undersampling rates prove the advantages of the proposed method in subjective visual quality and objective evaluation (peak signal to noise ratio and structure similarity index). The performance of this method is not only superior to the existing traditional CS-MRI methods, but also competitive with the existing deep neural network (DNN) based models. The code will be released at https://github.com/HaoHou-98/PNCS.

Abstract Background It is inevitable to change the function or expression of genes during the environmental adaption of species. Both the giant panda (Ailuropoda melanoleuca) and red panda (Ailurus fulgens) belong to Carnivora and have developed similar adaptations to the same dietary switch to bamboos at the morphological and genomic levels. However, the genetic adaptation at the gene expression level is unclear. Therefore, we aimed to examine the gene expression patterns of giant and red panda convergent specialized bamboo-diets. We examined differences in liver and pancreas transcriptomes between the two panda species and other non-herbivorous species. Results The clustering and PCA plots suggested that the specialized bamboo diet may drive similar expression shifts in these two species of pandas. Therefore, we focused on shared liver and pancreas DEGs (differentially expressed genes) in the giant and red panda relative to other non-herbivorous species. Genetic convergence occurred at multiple levels spanning carbohydrate metabolism, lipid metabolism, and lysine degradation. The shared adaptive convergence DEGs in both organs probably be an evolutionary response to the high carbohydrate, low lipid and lysine bamboo diet. Convergent expression of those nutrient metabolism-related genes in both pandas was an intricate process and subjected to multi-level regulation, including DNA methylation and transcription factor. A large number of lysine degradation and lipid metabolism related genes were hypermethylated in promoter regions in the red panda. Most genes related to carbohydrate metabolism had reduced DNA methylation with increased mRNA expression in giant pandas. Unlike the red panda, the core gene of the lysine degradation pathway (AASS) doesn’t exhibit hypermethylation modification in the giant panda, and dual-luciferase reporter assay showed that transcription factor, NR3C1, functions as a transcriptional activator in AASS transcription through the binding to AASS promoter region. Conclusions Our results revealed the adaptive expressions and regulations of the metabolism-related genes responding to the unique nutrients in bamboo food and provided data accumulation and research hints for the future revelation of complex mechanism of two pandas underlying convergent adaptation to a specialized bamboo diet.

It was necessary to improve the storage quality and commodity quality of Z. jujube ‘Jingcang1’. The effects of different concentrations of salicylic acid (10, 30, 50 mmol/L SA) combined with microporous fresh-keeping bag treatment on the preservation effect of Z. jujube ‘Jingcang1’ were investigated. The results showed that SA treatment could effectively delay the decline of storage quality of' Z. jujube ‘Jingcang1’. After 14 days of storage, the Z. jujube ‘Jingcang1’, which was not packed with microporous plastic bag, was obviously dehydrated and shriveled and its quality deteriorated seriously. The fresh-keeping effect of Z. jujube ‘Jingcang1’ packed with microporous plastic bag was not ideal. Packaging with microporous plastic bag combined with 30 mmol/L SA treatment had the best effect, which could prolong its storage period by about 40 days compared with that of the group without microporous plastic bag. Compared with the control group, microporous plastic bag combined with 30 mmol/L SA treatment can effectively delay the consumption of total soluble solid, titratable acid and other nutrients in the fruit of Z. jujube ‘Jingcang1’, delay the dehydration shrinkage, the decrease of brightness and fruit firmness of the fruit during storage, and maintain a relatively high content of soluble sugar, ascorbic acid, total polypheno, soluble protein and other nutrients and antioxidant activity after storage (P

Indoor environmental quality (IEQ) significantly affects human health and wellbeing. Therefore, continuous IEQ monitoring and feedback is of great concern in both the industrial and academic communities. However, most existing studies only focus on developing sensors that cost-effectively promote IEQ measurement while ignoring interactions between the human side and IEQ monitoring. In this study, an intelligent IEQ monitoring and feedback system—the Intelligent Built Enviroment (IBEM)—is developed. Firstly, the IBEM hardware instrument integrates air temperature, relative humidity, CO2, particulate matter with an aerodynamic diameter no greater than 2.5 μm (PM2.5), and illuminance sensors within a small device. The accuracy of this integrated device was tested through a co-location experiment with reference sensors; the device exhibited a strong correlation with the reference sensors, with a slight deviation (R2 > 0.97 and slopes between 1.01 and 1.05). Secondly, a wireless data transmission module, a cloud storage module, and graphical user interfaces (i.e., a web platform and mobile interface) were built to establish a pathway for dataflow and interactive feedback with the occupants of the indoor environments. Thus, the IEQ parameters can be continuously monitored with a high spatiotemporal resolution, interactive feedback can be induced, and synchronous data collection on occupant satisfaction and objective environmental parameters can be realized. IBEM has been widely applied in 131 buildings in 18 cities/areas in China, with 1188 sample locations. Among these applications, we report on the targeted IEQ diagnoses of two individual buildings and the exploration of relationships between subjective and objective IEQ data in detail here. This work demonstrates the great value of IBEM in both industrial and academic research.

Abstract Human pluripotent stem cell (hPSC)-induced NK (iNK) cells are a source of off-the-shelf cell products for universal immune therapy. Conventional methods for iNK cell regeneration from hPSCs include embryoid body (EB) formation and feeder-based expansion steps, which are time-consuming and cause instability and high costs of manufacturing. Here, we develop an EB-free, organoid aggregate method for NK cell regeneration from hPSCs. In a short time-window of 27-day induction, millions of hPSC input can output over billions of iNK cells without the necessity of NK cell expansion feeders. The iNK cells highly express classical toxic granule proteins, apoptosis-inducing ligands, as well as abundant activating and inhibitory receptors. Functionally, the iNK cells eradicate human tumor cells via mechanisms of direct cytotoxicity, apoptosis, and antibody-dependent cellular cytotoxicity. This study provides a reliable scale-up method for regenerating human NK cells from hPSCs, which promotes the universal availability of NK cell products for immune therapy.

Panzao (Ziziphus jujuba Mill. cv. ‘Jingcang1’) have a short shelf-life, which hampers their marketability. To investigate effects of combined pre- and postharvest treatments on quality and softening during storage, preharvest Guomantian foliar fertilizer (FF) spray and postharvest salicylic acid (SA) dip were applied to panzao. By day 63 of storage, fruit firmness responded to treatment in the order of FF+SA treatment > FF or SA treatments > Control. All treatments inhibited fruit reddening during storage and, compared with those of the control, alleviated the decline in total soluble solids, ascorbic acid, and phenol contents and decreased the oxidative aging process, which was consistent with the change in firmness. The FF+SA treatment reduced the activities of amylase, cellulase, polygalacturonase, and β-glucosidase, which decreased the degradation of macromolecules including starch, cellulose, and pectin. Principal component analysis showed that FF+SA exhibited a synergistic effect and was the most effective treatment for maintaining fruit quality during storage. FF increased accumulation of nutrients during growth and improved quality, while SA delayed the decline in fruit quality and reduced softening. These results provide the theoretical basis and technical knowledge for improving the shelf-life of panzao during storage and long-distance transportation.

Summary: Foraging on nocturnally migrating birds is one of the most challenging foraging tasks in the animal kingdom. Only three bat species (e.g., Ia io) known to date can prey on migratory birds. However, how these bats have exploited this challenging dietary niche remains unknown. Here, we demonstrate that I. io hunts at the altitude of migrating birds during the bird migration season. The foraging I. io exhibited high flight altitudes (up to 4945 m above sea level) and high flight speeds (up to 143.7 km h−1). I. io in flight can actively prey on birds in the night sky via echolocation cues. Genes associated with DNA damage repair, hypoxia adaptation, biting and mastication, and digestion and metabolism have evolved to adapt to this species’ avivorous habits. Our results suggest that the evolution of behavioral innovation and genomic novelty are associated with the exploitation of challenging dietary opportunities.

Red fluorescent proteins are useful as morphological markers in neurons, often complementing green fluorescent protein-based probes of neuronal activity. However, commonly used red fluorescent proteins show aggregation and toxicity in neurons or are dim. We report the engineering of a bright red fluorescent protein, Crimson, that enables long-term morphological labeling of neurons without aggregation or toxicity. Crimson is similar to mCherry and mKate2 in fluorescence spectra but is 100 and 28% greater in molecular brightness, respectively. We used a membrane-localized Crimson-CAAX to label thin neurites, dendritic spines and filopodia, enhancing detection of these small structures compared to cytosolic markers.

The intrinsic factors that determine the fundamental traits of engraftment ability and multi-lineage potential of hematopoietic stem cells (HSCs) remain elusive. The induction of bona fade HSCs from pluripotent stem cells (PSCs) in dishes is urgently demanded but remains a great challenge in translational medicine. Runx1, Hoxa9, Hlf, and Hoxa7 are developmentally co-expressed during endothelial-to-hematopoietic transition and adult haematopoiesis. However, the expression of these factors fails to be turned on during in vitro hematopoietic induction from PSCs. Here, we established an inducible gene over-expression embryonic stem cell (ESC) line in which exogenous Runx1, Hoxa9, Hlf, and Hoxa7 genes were tandemly knocked in. A population of induced hematopoietic progenitor cells (iHPCs) expressing Kit and Sca1 surface markers were successfully obtained in vitro from the gene edited-ESC line. Upon transplantation of the Runx1-Hoxa9-Hlf-Hoxa7 ESC-derived iHPCs into irradiated immunodeficient mice, they can dominantly contribute to B cells, low proportions of T cells and myeloid cells. However, Runx1-Hoxa9-Hlf ESC-derived iHPCs only produced B lineage cells with extremely low contributions. Our study unveils that the coordination of Runx1, Hoxa9, Hlf, and Hoxa7 led to generation of the hematopoietic progenitors with the capacity of multi-lineage hematopoietic reconstitution in the immunodeficient recipient mice.

ObjectiveThe COVID-19 lockdown extended premature rupture of membranes (PROM) expectant time among nulliparas and increased the risk of term neonatal complications. This study investigated the impact of term nulliparas with PROM delays at home on neonatal outcomes during the COVID-19 lockdown period, considering the clinical diagnostic application of maternal C-reactive protein (CRP).MethodsThis study collected 505 term nulliparous women who underwent PROM at home from five provinces in a non-designated hospital of China in 2020. We analyzed PROM maternal information at home and neonatal complications in the COVID-19 regional lockdown and compared related information in the national lockdown. Poisson regression models estimated the correlation of PROM management at home, maternal CRP, and neonatal morbidity. We constructed two diagnostic models: the CRP univariate model, and an assessed cut-off value of CRP in the combined model (CRP with PROM waiting time at home).ResultsIn the regional lockdown, PROM latency at home and the severity of neonatal complications were extended and increased lower than in the nationwide lockdown, but term neonatal morbidity was not reduced in the COVID-19 localized lockdown. Prolonged waiting time at home (≥8.17 h) was associated with increasing maternal CRP values and neonatal morbidity (adjusted risk ratio 2.53, 95% CI, 1.43 to 4.50, p for trend

Post-translational protein modifications can affect lifespan and aging but age-dependent ubiquitylation changes have not yet been systematically characterized. Here, the authors analyze age-related proteome and ubiquitylome dynamics in Drosophila and identify increasing H2A ubiquitylation as a conserved aging marker.

Various research works have been carried out on cement and lime stabilization of lateritic soil. The production of cement is too expensive and environmentally unfriendly due to the required huge embodied energy as well as the environmental pollution resulting in ozone layer depletion. In order to minimize these shortcomings, researchers are exploring a sustainable alternative to cement. There is limited scholarly work done on the use of a combination of agro-waste ashes for the stabilization of lateritic soil. This study is aimed at examining the performance of lateritic soil stabilized with a combination of bone ash (BA) and palm bunch ash (PBA) for sustainable building applications. Different cube sizes (50 mm3, 70 mm3, and 100 mm3) were used to produce lateritic bricks of different matrices which were used to obtain the compressive strength test results. Addition of a minimal percentage of BA (2%) and PBA (2%) improved the compressive strength of lateritic bricks by 129% compared to the natural lateritic soil. Brick specimen produced with the smallest mould size (2500 mm2) gave higher compressive strength compared to larger mould size. The findings of this study confirm the potential of using lateritic soil stabilized with a minimal percentage of BA (2%) and PBA (2%) for sustainable building applications.

Hematopoietic stem cells (HSC) are dominantly quiescent under homeostasis, which is a key mechanism of maintaining the HSC pool for life-long hematopoiesis. Dormant HSC are poised to be immediately activated in certain conditions and can return to quiescence after homeostasis has been regained. At present, the molecular networks of regulating the threshold of HSC dormancy, if existing, remain largely unknown. Here, we show that deletion of Nupr1, a gene preferentially expressed in HSC, activated quiescent HSC under homeostasis, which conferred a competitive engraftment advantage for these HSC without compromising their stemness or multi-lineage differentiation capacity in serial transplantation settings. Following an expansion protocol, the Nupr1-/- HSC proliferated more robustly than their wild-type counterparts in vitro. Nupr1 inhibits the expression of p53 and rescue of this inhibition offsets the engraftment advantage. Our data reveal a new role for Nupr1 as a regulator of HSC quiescence, which provides insights for accelerating the engraftment efficacy of HSC transplantation by targeting the HSC quiescence-controlling network.

OBJECTIVES: To investigate the effects of an intratympanic injection of dexamethasone combined with gentamicin on the expression level of serum P0 protein antibodies in patients with Meniere’s disease (MD). METHODS: A total of 136 patients with MD treated in our hospital were enrolled in this study. Among them, 68 patients were treated with an intratympanic injection of dexamethasone combined with gentamicin (observation group). Another 68 patients were treated with gentamicin alone (control group). RESULTS: After treatment, the expression levels of IgG and IgM in the two groups significantly decreased (p0.05). CONCLUSION: For patients with MD, dexamethasone combined with gentamicin can reduce the incidence of vertigo, tinnitus, and gait instability, but it has no effect on the efficacy or number of vertigo attacks 6 months after treatment. Therefore, the levels of myelin P0 protein antibodies after treatment can be used as predictors of vertigo at 6 months after treatment.

At present,the influence of the image sustained damage is often neglected in the objective method of the quality of user experience in video service.In view of this problem,the effect of the visible duration of visual impairment on the quality of the user experience was discussed.At the same time,the objective reason of continuous damage was analyzed,and a Bayesian network was constructed which included hierarchical objective index and subjective evaluation result.On this basis,a no-reference evaluation method for the quality of video service experience was proposed,and the accuracy of the method is verified by subjective test.

In the field of hematopoietic regeneration, deriving hematopoietic stem cells (HSCs) from pluripotent stem cells with engraftment potential is the central mission. Unstable hematopoietic differentiation protocol due to variation factors such as serums and feeder cells, remains a major technical issue impeding the screening of key factors for the derivation of HSCs. In combination with hematopoietic cytokines, UM171 has the capacity to facilitate the maintenance and expansion of human primary HSCs in vitro. Here, using a serum-free, feeder-free, and chemically defined induction protocol, we observed that UM171 enhanced hematopoietic derivation through the entire process of hematopoietic induction in vitro. UM171 facilitated generation of robust CD34+CD45+ derivatives that formed more and larger sized CFU-GM as well as larger sized CFU-Mix. In our protocol, the derived hematopoietic progenitors failed to engraft in NOG mice, indicating the absence of long-term HSC from these progenitors. In combination with other factors and protocols, UM171 might be broadly used for hematopoietic derivation from human pluripotent stem cells in vitro.

In order to protect data privacy whilst allowing efficient access to data in multi-nodes cloud environments, a parallel homomorphic encryption (PHE) scheme is proposed based on the additive homomorphism of the Paillier encryption algorithm. In this paper we propose a PHE algorithm, in which plaintext is divided into several blocks and blocks are encrypted with a parallel mode. Experiment results demonstrate that the encryption algorithm can reach a speed-up ratio at about 7.1 in the MapReduce environment with 16 cores and 4 nodes.

ObjectivesCoal workers’ pneumoconiosis is caused by exposure to respirable coal mine dust. The self-protection of underground coal miners (UCM) plays an irreplaceable role against this threat. The purpose of this study was to evaluate the predictors of self-protective behaviour (SPB) in Chinese UCM based on the health belief model (HBM).MethodsA cross-sectional study was conducted among 341 UCM in January 2016 in Pingdingshan City, Henan Province, People’s Republic of China. Data was collected using a self-reported questionnaire, which included questions on sociodemographic characteristics, HBM variables and SPB. An exploratory factor analysis of the principal components with varimax rotation was carried out on the HBM-related items. Pearson’s correlation and multiple linear regression analysis were used to assess the relationship between sociodemographic characteristics, HBM variables and SPB.ResultsMultiple regression analysis demonstrated the monthly income (B=0.403, p=0.001), the internal cues to action (B=0.380, p

Hyperhomocysteinemia, a risk factor for vascular disease, is associated with metabolic syndrome. Our study was aimed at exploring the effect of long-term hyperhomocysteinemia with metabolic disturbances on vascular remodeling. We also studied oxidative stress and expression of PPARγ in the coronary arteriole as a possible mechanism underlying vascular remodeling. Rats were treated with standard rodent chow (Control) or diet enriched in methionine (Met) for 48 weeks. Plasma homocysteine, blood glucose, serum lipids, malondialdehyde (MDA), superoxide dismutase (SOD), and nitric oxide (NO) levels were measured. Coronary arteriolar and carotid arterial remodeling was assessed by histomorphometric techniques and the expression of PPARγ in vessel wall was investigated. In Met group, an increase in the level of fasting blood glucose, serum triglyceride, total cholesterol, MDA, and NO, a decline in the serum SOD level, and increased collagen deposition in coronary and carotid arteries were found. Moreover, we detected decreased expression of PPARγ in the coronary arterioles in Met group. In summary, our study revealed metabolic disturbances in this model of long-term hyperhomocysteinemia together with vascular remodeling and suggested that impaired oxidative stress, endothelium dysfunction, and decreased PPARγ expression in the vessel wall could be underlying mechanisms.

Generating engraftable hematopoietic stem cells (HSCs) from pluripotent stem cells (PSCs) is an ideal approach for obtaining induced HSCs for cell therapy. However, the path from PSCs to robustly induced HSCs (iHSCs) in vitro remains elusive. We hypothesize that the modification of hematopoietic niche cells by transcription factors facilitates the derivation of induced HSCs from PSCs. The Lhx2 transcription factor is expressed in fetal liver stromal cells but not in fetal blood cells. Knocking out Lhx2 leads to a fetal hematopoietic defect in a cell non-autonomous role. In this study, we demonstrate that the ectopic expression of Lhx2 in OP9 cells (OP9-Lhx2) accelerates the hematopoietic differentiation of PSCs. OP9-Lhx2 significantly increased the yields of hematopoietic progenitor cells via co-culture with PSCs in vitro. Interestingly, the co-injection of OP9-Lhx2 and PSCs into immune deficient mice also increased the proportion of hematopoietic progenitors via the formation of teratomas. The transplantation of phenotypic HSCs from OP9-Lhx2 teratomas but not from the OP9 control supported a transient repopulating capability. The upregulation of Apln gene by Lhx2 is correlated to the hematopoietic commitment property of OP9-Lhx2. Furthermore, the enforced expression of Apln in OP9 cells significantly increased the hematopoietic differentiation of PSCs. These results indicate that OP9-Lhx2 is a good cell line for regeneration of hematopoietic progenitors both in vitro and in vivo.

The Caledonian Palaeohigh is an important gas exploration domain of Sinian and Lower Paleozoic in western Central Sichuan Basin where gas discoveries have been made successively in recent years. In order to sort out the relationship between the tectonic evolution of this Palaeohigh and hydrocarbon accumulation there, we carried out a new round of research based on previous study results. The evolution history of this Palaeohigh can be divided into seven episodes: the Late Sinian overall tilting and the youth form development of this Palaeohigh, the Cambrian-Ordovician syndepositional uplifting, the Silurian joint uplifting, the Devonian-Carboniferous uplifting and erosion, the Permian overall subsidence and deposition of regional caprock, the Triassic-Jurassic migration of structural high of the eastern segment of this Palaeohigh, and Cretaceous-Neogene strong deformation of the western segment of this Palaeohigh. The hydrocarbon accumulation in the Sinian-Lower Paleozoic experienced three evolution stages, namely the formation of ancient oil reservoirs in the Silurian-Triassic period, the development of the paleo-gas reservoirs in the Jurassic-Oligocene period, and differential evolution of gas reservoirs since the Miocene era. The Sinian-Lower Paleozoic paleo-reservoirs mainly occur in the Leshan-Ziyang-Gaoshiti-Longnǚsi zone and in the area to its north. The tectonic movement of the western segment of the Palaeohigh was strong, while its eastern segment was relatively stable since the later Himalayan epoch, which is favorable for gas accumulation and preservation. It is believed that the Gaoshiti-Moxi-Longnǚsi structural belt and its northern flank are not only favorable for the development of structural gas reservoirs in the Lower Paleozoic but also for gas reservoirs of karstic-lithologic type on the top of the Cambrian and Ordovician. The later type will be the major exploration target of this area in the future.

Natural hematopoietic stem cells (HSC) are susceptible and tend to lose stemness, differentiate, or die on culture condition in vitro, which adds technical challenge for maintaining bona fide HSC-like cells, if ever generated, in protocol screening from pluripotent stem cells. It remains largely unknown whether gene-editing of endogenous genes can genetically empower HSC to endure the culture stress and preserve stemness. In this study, we revealed that both NUP98-HOXA10HD fusion and endogenous Nras mutation modifications (NrasG12D) promoted the engraftment competitiveness of HSC. Furthermore, the synergy of these two genetic modifications endowed HSC with super competitiveness in vivo. Strikingly, single NAV-HSC successfully maintained its stemness and showed robust multi-lineage engraftments after undergoing the in vitro culture. Mechanistically, NUP98-HOXA10HD fusion and NrasG12D mutation distinctly altered multiple pathways involving the cell cycle, cell division, and DNA replication, and distinctly regulated stemness-related genes including Hoxa9, Prdm16, Hoxb4, Trim27, and Smarcc1 in the context of HSC. Thus, we develop a super-sensitive transgenic model reporting the existence of HSC at the single cell level on culture condition, which could be beneficial for protocol screening of bona fide HSC regeneration from pluripotent stem cells in vitro.