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Simple Summary: Muscle development plays a pivotal role in the growth process of chickens. Clarifying the mechanisms of muscle development holds significant scientific and economic value. In this study, transcriptome sequencing was conducted on muscle tissues from two broilers with differing growth rates, identifying Insulin–like growth factor 2 mRNA binding protein 1 (IGF2BP1) as a potential participant in chicken muscle development via bioinformatics analysis. The identified genes were further validated using overexpression and knockdown approaches. It was found that IGF2BP1 is highly expressed in chicken muscle tissues, and the experimental results indicated that IGF2BP1 expression promotes the proliferation and differentiation of chicken primary myoblasts (CPMs). These findings offer new insights into the regulation of muscle development. Muscle development is a multifaceted process influenced by numerous genes and regulatory networks. Currently, the regulatory network of chicken muscle development remains incompletely elucidated, and its molecular genetic mechanisms require further investigation. The Longsheng-Feng chicken, one of the elite local breeds in Guangxi, serves as an excellent resource for the selection and breeding of high-quality broiler chickens. In this study, we conducted transcriptome sequencing of the pectoral muscles of Longsheng-Feng chickens and AA broiler chickens with different growth rates. Through comprehensive bioinformatics analysis, we identified differentially expressed genes that affect muscle growth and showed that IGF2BP1 is a key participant in chicken muscle development. Subsequently, we employed QRT-PCR, EdU staining, and flow cytometry to further investigate the role of IGF2BP1 in the proliferation and differentiation of chicken myogenic cells. We identified 1143 differentially expressed genes, among which IGF2BP1 is intimately related to the muscle development process and is highly expressed in muscle tissues. Overexpression of IGF2BP1 significantly promotes the proliferation and differentiation of chicken primary myoblasts, while knockdown of IGF2BP1 significantly inhibits these processes. In summary, these results provide valuable preliminary insights into the regulatory roles of IGF2BP1 in chicken growth and development. [ABSTRACT FROM AUTHOR]

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Copyright of Acta Edulis Fungi is the property of Acta Edulis Fungi and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Article impact statement: Conservation mycology is a newly introduced discipline relevant to conservation biologists, mycologists, ecologists, and conservation policy. [ABSTRACT FROM AUTHOR]