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Background: Doxorubicin (DOX) is an effective anticancer drug, but it has a problem of cardiotoxicity that cannot be ignored. Ca2+/calmodulin‐dependent protein kinase II (CaMKII) is tightly associated with the pathological progression of DOX‐induced cardiotoxicity. Ubiquitin‐specific protease 10 (USP10) plays an important role in many biological processes and cancers. However, its association with DOX‐induced cardiotoxicity and CaMKII remains unclear. Methods: H9C2 cells, HL‐1 cells and C57BL/6 mice were used to establish the DOX‐induced cardiotoxicity model, and the CaMKII‐specific inhibitor KN‐93 and USP10 specific inhibitor Spautin‐1 were used to observe the CaMKII and USP10 effect. In cell experiments, CCK‐8 method was used to assess cell viability, LDH kit was used to assess lactate dehydrogenase expression, DCFH‐DA staining was used to observe changes in active oxygen content, TUNEL staining was used to observe cell apoptosis, and Western blotting method was used to detect relevant protein markers. The expression of p‐CaMKII and USP10 was assessed by immunofluorescence staining. In animal experiments, mouse echocardiograph was used were used to evaluate cardiac function, and HE staining and Masson staining were used to evaluate myocardial injury. Cardiomyocyte apoptosis was detected by TUNEL staining. Western blotting method was used to detect relevant protein markers. Results: Our results demonstrated that activation of CaMKII and inhibition of USP10 pathway related to DOX‐induced cardiotoxicity. Inhibition of CaMKII with KN‐93 ameliorated DOX‐induced cardiac dysfunction and cytotoxicity. In addition, CaMKII inhibition prevented DOX‐induced apoptosis and ubiquitination. Furthermore, CaMKII inhibition increased USP10 expression in DOX‐treated mouse hearts, H9C2 cells and HL‐1 cells. At last, the USP10 inhibitor, Spautin‐1, blocked the regulatory effect of CaMKII inhibition on apoptosis and ubiquitination in DOX‐induced cardiotoxicity. Conclusion: Our findings revealed that DOX‐induced myocardial apoptosis and activated CaMKII through cellular and animal levels, while providing a novel probe into the mechanism of CaMKII action: promoting ubiquitination by inhibiting USP10 aggravated apoptosis. [ABSTRACT FROM AUTHOR]

Social commerce blurs the boundary between online social interaction and online shopping. The emergence of video streams introduces novel marketing modalities to social commerce. However, there is a paucity of comprehensive studies investigating the impact of emerging marketing techniques such as short videos and live streaming on consumer purchase intention. This study employs Bourdieu's conceptual framework to construct a Field Theory-based model, investigating the impact of atmospheric and capital characteristics of social commerce platforms on consumer purchase intention through affective and rational pathways, respectively. A survey involving 515 Chinese social commerce consumers demonstrates that atmospheric characteristics (emotion and social presence) and capital characteristics (information quality and quantity) in video streams enhance similarity and power. Both similarity and power are associated with an increase in consumer purchase intention. This study validates the dual-path influence of social commerce characteristics and discusses theoretical and managerial implications. [ABSTRACT FROM AUTHOR]

Rhamnolipids, simply divided into mono‐rhamnolipids and di‐rhamnolipids, their physicochemical properties are structure‐dependent. This study explored to increase the proportion of di‐rhamnolipids biosynthesized from Pseudomonas aeruginosa and evaluate the relationship between activity and di‐rhamnolipids proportions. P. aeruginosa SGrhlC was constructed by increasing the rhlC gene in P. aeruginosa SG. HPLC‐MS results indicated that SGrhlC produced more di‐rhamnolipids (62.32%) than that of the wild‐type strain SG (45.24%). Both the species and proportion of di‐rhamnolipids were increased, mainly Rha‐Rha‐C8‐C10 and Rha‐Rha‐C10‐C10. The rhamnolipids produced by SGrhlC was thermostable and salt‐tolerant. The SGrhlC rhamnolipids decreased surface tension of water to 27.0 mN/m with a critical micelle concentration (CMC) of 50 mg/L and emulsified crude oil with an emulsification index of 66.9 ± 1.5%. The SGrhlC rhamnolipids exhibited antimicrobial activity against Staphylococcus aureus and Cladosporium sp. with IC50 (half maximal inhibitory concentration) values less than 15 mg/L, and washed 73.02% ± 1.77% of oil from petroleum‐contaminated soil. Physicochemical activities of three bacterial rhamnolipids varied with their di‐rhamnolipids proportions. Results indicated that the higher proportions of di‐rhamnolipids were, the lower CMC, better surface activity and higher washing oil rate were, while the weaker emulsifying activity and lower antimicrobial activity were. The SGrhlC rhamnolipids showed better surface activity and a lower critical micelle concentration, which was superior for wetting, foaming, desorbing and dispersing. This study revealed that P. aeruginosa can be genetically regulated to biosynthesize rhamnolipids with specific structure. Perspectives of the customized biosynthesis and applications of rhamnolipids were also discussed. [ABSTRACT FROM AUTHOR]