Search

The optimal conditions for the preparation of superparamagnetic chitosan plasmid (pReceiver‐M29‐VEGF165/DH5a) gelatin microspheres (SPCPGMs) were determined. Then, the performance of the SPCPGMs during neovascularization was evaluated in vivo. The SPCPGMs were prepared through a cross‐linking curing method and then filled into the hollow scaffold of an artificial bone. Neovascularization at the bone defect position was histologically examined in samples collected 2, 4, 6, and 8 weeks after the operation. The cellular magnetofection rate of superparamagnetic chitosan nanoparticles/plasmid (pReceiver‐M29‐VEGF165/DH5a) complexes reached 1–3% under static magnetic field (SMF). Meanwhile, the optimal conditions for SPCPGM fabrication were 20% Fe3O4 (w/v), 4 mg of plasmid, 5.3 mg of glutaraldehyde, and 500 rpm of emulsification rotate speed. Under oscillating magnetic fields (OMFs), 4–6 μg of plasmids was released daily for 21 days. Under the combined application of SMF and OMF, evident neovascularization occurred at the bone defect position 6 weeks after the operation. This result is expected to provide a new type of angiogenesis strategy for the research of bone tissue engineering.

New rare-earth half-metallic ferromagnets X 0.75 Eu 0.25 O ( X =Ca, Sr and Ba) with the NaCl structure are predicted based on the first-principles calculations. These materials only have up-spin subbands at the Fermi level and their net magnetic moments are all 7.00 µ B per unit cell, which exhibits the evident half-metallic characteristics. Their energy gaps and half-metallic gaps are both wide so that they all have high Curie temperatures and stable half-metallicity, holding great potential applications in spintronics. From the calculated formation energies, it is possible to prepare Sr 0.75 Eu 0.25 O and Ba 0.75 Eu 0.25 O experimentally. However, it may be difficult to prepare Ca 0.75 Eu 0.25 O in experiments. The magnetic moments and the half-metallicity of X 0.75 Eu 0.25 O mainly originate from the spin-polarization of Eu 4f-orbitals, which are caused by the strong octahedral crystal field in the ligand compound ML 6 consisting of the Eu-ion and six O-ions around it.

205 surface pollen samples from different communities in Northern China were analyzed to understand the quantitative relationship between pollen and its original vegetation. Pollen analysis and vegetation investigation show that the pollen assemblages differ a lot in different vegetation regions. Arboreal pollen account for more than 30% in temperate broad-deciduous forests region. In temperate steppe regions, herb pollen percentages are more than 90%, where Artemisia and Chenopodiaceae are dominant pollen types with Artemisia percentages more than 30%. In temperate desert, Chenopodiaceae pollen percentages are more than Artemisia, where ferns are rare. Cyperaceae pollen percentages are more than 20% in sub-alpine or cold meadows. The relations between pollen percentages and vegetation cover indicate that most arboreal pollen shows a close relationship with parent plant covers, most shrubby pollen types have more or less correlations, but most herbs do not show clear correlations. For arboreal pollen types, Picea pollen shows the closest correlation with spruce trees coverage, then is Quercus and Carpinus. Betula, Larix and Juglans have also high correlation coefficients with their plants coverage, but Betula pollen is of overrepresented pollen type and more than 40% in birch forest, while Larix and Juglans pollen is underrepresented and pollen percentages are more than 10% in Larix or Juglans pure forests. Pinus is of overrepresented pollen type, and pollen percentages have some relations with plants cover. Pine forest might present when Pinus pollen percentages are more than 30%. The relations between Ulmus and Populus pollen percentages and vegetation cover are not close, where they are mixed with other arbors, they cannot be recorded easily, but if their pollen percentages are more than 1%, Ulmus or Populus trees should exist. For shrubby pollen types, the correlation between Vitex pollen percentages and vegetation cover is the highest, then is Corylus, Tamariaceae and Nitraria, and their pollen percentages are less than 1% where original plant are absent. Caragana and Spiraea pollen percentages have some relations with vegetation cover. The relations between pollen percentages and vegetation cover are not clear for Rosaceae and Saxifragaceae. For herb pollen types, Cyperaceae pollen has the closest correlation with vegetation cover, where pollen percentages are more than 20% when Cyperaceae are constructive or dominant species in vegetation, and pollen percentages are less than 5% where Cyperaceae are not constructive or dominant species (cover less than 30%). Artemisia and Chenopodiaceae pollen percentages mainly have close relations with ecological regions. The relations between pollen percentages and cover are not clear for Gramineae, Leguminosae and Compositae.