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Current treatments for neurodegenerative diseases and neural injuries face major challenges, primarily due to the diminished regenerative capacity of neurons in the mammalian CNS as they mature. Here, we investigated the role of Ezh2, a histone methyltransferase, in regulating mammalian axon regeneration. We found that Ezh2 declined in the mouse nervous system during maturation but was upregulated in adult dorsal root ganglion neurons following peripheral nerve injury to facilitate spontaneous axon regeneration. In addition, overexpression of Ezh2 in retinal ganglion cells in the CNS promoted optic nerve regeneration via both histone methylation-dependent and -independent mechanisms. Further investigation revealed that Ezh2 fostered axon regeneration by orchestrating the transcriptional silencing of genes governing synaptic function and those inhibiting axon regeneration, while concurrently activating various factors that support axon regeneration. Notably, we demonstrated that GABA transporter 2, encoded by Slc6a13, acted downstream of Ezh2 to control axon regeneration. Overall, our study underscores the potential of modulating chromatin accessibility as a promising strategy for promoting CNS axon regeneration., Introduction Axon degeneration and neuronal cell death are common consequences of neurodegenerative diseases and neural injuries. Unfortunately, current clinical therapeutics for neural injuries and neurodegenerative diseases still fall short of [...]

Objective: Based on data mining, Wang Xun's medication rule in the treatment of dysentery was discussed. Methods: the traditional Chinese medicine used in the prescriptions for dysentery in Wang Xun's "Tzu hang Ji Sanyuan Puji Fang" was counted, the names of the drugs were standardized, and their properties, taste, efficacy and meridian tropism were investigated respectively. The database was established with the help of Microsoft Excel 2016, SPSS statistic 24.0 and SPSS modeler 18.0 computer software, and the frequency analysis and high-frequency drug association rules were carried out Analysis, cluster analysis. Results: 44 prescriptions of Wang Xun's dysentery were sorted out and 64 traditional Chinese medicines were used, of which 22 were high-frequency drugs (drugs with frequency ≥ 5%). The top ten drugs were Cheqianzi, Danggui, liquorice, Muxiang, white peony, Fructus aurantii, areca, Poria cocos, radish and rhubarb. The correlation analysis produced "Raphanus seed→Plantago asiatica, Bitter orange→liquorice, tangerine peel→Fructus aurantii, Fructus aurantii→Cheqianzi, areca There are 15 associations of 2 kinds of drugs, such as hammer, Cheqianzi, Fructus aurantii, Muxiang, and so on. Cluster analysis shows that there are 4 cluster formulas: Angelica, Cheqianzi, Raphani, Fructus aurantii, white peony, liquorice, areca, red peony, tangerine peel, rhubarb, Fructus aurantii, Magnolia officinalis and Coptis. Conclusion: the method of data mining is to study the rule of Wang's prescription in the treatment of dysentery, so as to summarize the characteristics of Wang's prescription in the treatment of dysentery, which has guiding significance for clinical treatment of dysentery. [ABSTRACT FROM AUTHOR]

Buckyballs (C60) are linked to one end and two ends of linear poly(ethylene oxide) (PEO) chains through highly efficient click chemistry to obtain giant amphiphilic molecules C60-PEO and C60-PEO-C60, respectively. C60-PEO and C60-PEO-C60 molecules are spread on water surface and then transferred to solid substrates with Langmuir-Blodgett (LB) film deposition approach. C60-PEO and C60-PEO-C60 exhibit fractal growth behavior on the solid substrate under certain conditions owing to the crystallization ability of PEO segment. PEO chain length and the end capped mode both affect the fractal growth pattern. [ABSTRACT FROM AUTHOR]