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An EMS (ethy methanesulfonate)-induced lethal etiolated (le) mutant obtained from the rice variety Zhongjian 100 was characterized by lethal etiolated phenotypes, with significantly reduced levels of chlorophyll a, chlorophyll b, total chlorophyll, and carotenoids. Additionally, the mutant displayed a significantly decreased number of chloroplast grana, along with irregular and less-stacked grana lamellae. The le mutant showed markedly diminished root length, root surface area, and root volume compared with the wild type. It also exhibited significantly lower catalase activity and total protein content, while peroxidase activity was significantly higher. Using the map-based cloning method, we successfully mapped the LE gene to a 48-kb interval between markers RM16107 and RM16110 on rice chromosome 3. A mutation (from T to C) was identified at nucleotide position 692 bp of LOC_Os03g59640 (ChlD), resulting in a change from leucine to proline. By crossing HM133 (a pale green mutant with a single-base substitution of A for G in exon 10 of ChlD subunit) with a heterozygous line of le (LEle), we obtained two plant lines heterozygous at both the LE and HM133 loci. Among 15 transgenic plants, 3 complementation lines displayed normal leaf color with significantly higher total chlorophyll, chlorophyll a, and chlorophyll b contents. The mutation in le led to a lethal etiolated phenotype, which has not been observed in other ChlD mutants. The mutation in the AAA+ domain of ChlD disrupted the interaction of ChlDle with ChlI as demonstrated by a yeast two-hybrid assay, leading to the loss of ChlD function and hindering chlorophyll synthesis and chloroplast development. Consequently, this disruption is responsible for the lethal etiolated phenotype in the mutant.

Identification of immunity-associated leucine-rich repeat receptor-like protein kinases (LRR-RLK) is critical to elucidate the LRR-RLK mediated mechanism of plant immunity. Here, we reported the map-based cloning of a novel rice SPOTTED-LEAF 41 (OsSPL41) encoding a putative LRR-RLK protein (OsLRR-RLK41/OsSPL41) that regulated disease responses to the bacterial blight pathogen Xanthomonas oryzae pv. oryzae (Xoo). An 8-bp insertion at position 865 bp in a mutant spotted-leaf 41 (spl41) allele led to the formation of purple-brown lesions on leaves. Functional complementation by the wild type allele (OsSPL41) can rescue the mutant phenotype, and the complementary lines showed similar performance to wild type in a number of agronomic, physiological and molecular indices. OsSPL41 was constitutively expressed in all tissues tested, and OsSPL41 contains a typical transmembrane domain critical for its localization to the cell membrane. The mutant exhibited an enhanced level of resistance to Xoo in companion of markedly up-regulated expression of pathogenesis-related genes such as OsPR10a, OsPAL1 and OsNPR1, while the level of salicylic acid was significantly increased in spl41. In contrast, the over-expression lines exhibited a reduced level of H2O2 and were much susceptible to Xoo with down-regulated expression of pathogenesis-related genes. These results suggested that OsSPL41 might negatively regulate plant immunity through the salicylic acid signaling pathway in rice.

Our study investigates the genetic mechanisms underlying the spotted leaf phenotype in rice, focusing on the spl43 mutant. This mutant is characterized by persistent reddish-brown leaf spots from the seedling stage to maturity, leading to extensive leaf necrosis. Using map-based cloning, we localized the responsible locus to a 330 Kb region on chromosome 2. We identified LOC_Os02g56000, named OsRPT5A, as the causative gene. A point mutation in OsRPT5A, substituting valine for glutamic acid, was identified as the critical factor for the phenotype. Functional complementation and the generation of CRISPR/Cas9-mediated knockout lines in the IR64 background confirmed the central role of OsRPT5A in controlling this trait. The qPCR results from different parts of the rice plant revealed that OsRPT5A is constitutively expressed across various tissues, with its subcellular localization unaffected by the mutation. Notably, we observed an abnormal accumulation of reactive oxygen species (ROS) in spl43 mutants by examining the physiological indexes of leaves, suggesting a disruption in the ROS system. Complementation studies indicated OsRPT5A's involvement in ROS homeostasis and catalase activity regulation. Moreover, the spl43 mutant exhibited enhanced resistance to Xanthomonas oryzae pv. oryzae (Xoo), highlighting OsRPT5A's role in rice pathogen resistance mechanisms. Overall, our results suggest that OsRPT5A plays a critical role in regulating ROS homeostasis and enhancing pathogen resistance in rice. [ABSTRACT FROM AUTHOR]

This paper is a summary which explores the effectiveness and evaluation of my development as a reflective practitioner. It focuses on development with two useful theories about learning and teaching. They are about deep learning & surface learning and levels of teaching. Actually, when I put these theories to practice reflectively, the result shows positive outcomes, it can improve students' motivation and raise students' interest in studying English and help me developing in my profession.

This study investigates the application of foam asphalt (FA) to enhance the compaction effectiveness of a hot in-place recycling asphalt mixture (HIR-AM) during the HIR process of old road surfaces. Initially, the process parameters for FA preparation were determined through expansion-rate and half-life tests. Subsequently, the study focused on evaluating the impact of FA on the compaction quality of HIR-AM. Performance assessments were conducted through rutting tests, low-temperature bending tests, Hamburg wheel tracking tests, dynamic modulus analyses, and various other experiments to evaluate the road performance of HIR-FAM. Finally, the research findings were validated through practical engineering applications, and the construction process for HIR-FAM was summarized. The research results reveal that the optimal foaming temperature for SBS asphalt is 170 °C, with an ideal water content of 1.7%. Under the same compaction temperature, HIR-FAM demonstrated a significant reduction in void content, ranging from 3.8% to 21.2% compared to HIR-AM. Moreover, a higher proportion of FA usage resulted in a more substantial decrease in void content. Compared to HIR-AM, HIR-FAM exhibited notable improvements, including an 11.6% increase in dynamic stability, a 13.4% enhancement in bending strength, a 13.3% increase in maximum bending strain, an 8.1% improvement in residual stability, and an 8.5% boost in freeze–thaw splitting strength. Furthermore, HIR-FAM demonstrated superior water-thermal stability and resistance to low-frequency loads. Paving a test road verified that the adoption of foam asphalt in thermal recycling led to a compaction density increase of over 0.79% compared to traditional in situ thermal recycling sections, with improved compaction uniformity. [ABSTRACT FROM AUTHOR]

For inconvenient emergency stop control with low reliability of existing electro-hydraulic control equipment of hydraulic support, an emergency stop control scheme for electro-hydraulic control system of hydraulic support was proposed which was based on C8051F020 single chip microcomputer. A transmission mode taking special RS485 bus as emergency stop signal channel was drawn up as well as concrete implementation scheme of interlocking of emergency stop and reset. Meanwhile, functions of fault location, fault diagnosis and power-off memory for emergency stop state of support controller were achieved by use of software. An electro-hydraulic control system of hydraulic support based on the emergency stop control scheme has run in a coal mine with good effect.

In view of problem of low automation degree of current electro-hydraulic control system for hydraulic supports, a centralized control system for hydraulic supports on fully-mechanized coal mining face was designed, designs of hardware and software of the system were introduced, and realizing process of centralized control for hydraulic supports was also expounded. The test results show that the system can realize centralized control of hydraulic supports, and has performances of stable field communication and high real-time, which can meet actual requirement of coal mine production.

For problem that hydraulic support controller was operated manually in most coal mines at present, which led to low working efficiency and poor operation safety, a hardware design scheme of hydraulic support controller for automatic working face was introduced, and realization schemes of centralized control function and self-diagnosis function of the controller were expounded. The controller, which adopts dual controlling cores and communication mode of dual RS485 buses, can monitor, process and upload information real-timely including roof pressure of hydraulic support, pushing displacement and position of shearer, and realize automatic shearer tracking, support pulling and support pushing according to technical command from terminal controller. The controller also realizes self-diagnosis function by use of communication mode of I2C bus, which can real-timely monitor and correct fault action signals of hydraulic support. The results of industrial test on the ground and fault simulation experiment verify correctness of design scheme of the controller.

To solve problem of lacking remote monitoring function of running status in existing electro-hydraulic control system for hydraulic support, a running status monitoring system for hydraulic support based on dual RS485 buses was developed. In the system, an enhanced RS485 serial communication mode is used with data transmission speed of 10 Mbit/s. Dual RS485 buses communication mode is used for collateral execution of online monitoring and remote control. Some anti-interference measures are used in hardware circuit design including opto-coupler isolation circuit, high voltage intrusion prevention circuit and fault protection circuit, which has passed surge immunity test according to GB/T 17626.5-1998. The test results in laboratory and field show that the system realizes remote and online monitoring of running status of hydraulic support with strong real-time performance and anti-interference performance.

The compaction and re-crushing characteristics of crushed gangue are important factors which affect the quality and effectiveness of the filling of the quarry. To study the compaction and re-crushing characteristics of the gangue particles, continuous grading and intermittent grading of two different structures were designed to study the bulk gangue particle size distribution. By conducting a side-limited uniaxial compression test on the crushed gangue, the compaction deformation parameters and particle re-crushing parameters of the samples under different axial pressure and grading conditions were calculated, the interaction between compaction and re-crushing was determined, and a compaction-re-crushing model of the crushed gangue was established. The following conclusions were obtained, (1) the axial displacement increment and fractal dimension of the graded crushed gangue are closely related to the graded structure of the skeletal particles; (2) the compaction stage of the graded crushed gangue can be divided into a fast compaction stage, a slow compaction stage, and a stable compaction stage—the fast compaction stage is significantly elastic, the slow compaction stage is more plastic, and the stable stage behavior approaches that of the original rock body; (3) the degree of crushing of the graded crushed gangue increases with an increase in axial stress, and the re-crushing of the specimen mainly occurs in the slow compaction stage; (4) the compaction-crushing-fractal evolution of the graded crushed gangue in the lateral limit compression process is established as the line of questioning. The physical significance of the parameters in the equation is discussed in this paper. The study can provide theoretical support and engineering guidance for the precise filling of a quarry and the prevention of later collapse. [ABSTRACT FROM AUTHOR]