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A series of nano-photocatalysts metal-organic frameworks (MOFs)/graphitic carbon nitride (CN) (named MOFCN- x) with high activity have been synthesized by in-situ growth method. Under visible light irradiation, MOFCN- x hybrids show enhanced photocatalytic activity for the debromination of polybromodiphenyl ethers (PBDEs) compared with CN. Among all the hybrids, MOFCN-2 shows the highest reaction rate, which is 3.3 times as high as that in CN. MOFCN- x photocatalysts own stable visible light activity after recycled experiment. It indicates that a moderate amount of MOFs in MOFCN- x can largely enhance the photocatalytic ability by improved visible light absorption, larger specific surface area and better photo-generated charge carriers separation and transfer capabilities. More interestingly, the debromination pathway of PBDEs by MOFCN- x shows obvious selectivity compared with pure CN that bromines at meta -positions are much more susceptible than those at the para - and ortho -positions. The possible photoreductive mechanism has been proposed. This study shows that nanocomposite MOFCN can be an excellent candidate for dealing with halogen pollutants by solar-driven. [Display omitted] [ABSTRACT FROM AUTHOR]

In this study, biogas and biogas slurry were simultaneously treated using two symbiotic systems: Chlorella vulgaris‐Ganoderma lucidum‐S395‐2 (endophytic bacteria) and Scenedesmus obliquus‐G. lucidum‐S395‐2. The influence of different mixed illumination (red and blue) intensity ratios on the algal symbionts' extracellular carbonic anhydrase activities was investigated, as well as the rates of microalgal growth and photosynthesis. The treatment performance was simultaneously assessed in terms of the efficiency of organic matter or nutrient removal and the level of CO2 absorption. The results indicated that red‐blue light combinations with an intensity ratio of 5:5 were optimal. When comparing the performance of the two symbiotic systems, the C. vulgaris‐G. lucidum‐S395‐2 symbiont co‐culture system achieved significantly improved photosynthetic rates, biomass growth, and treatment effects. Under the optimal treatment conditions, the organic matter and nutrient removal rates were 81.06% ± 7.06% for chemical oxygen demand, 82.32% ± 7.18% for total nitrogen, and 82.98% ± 7.26% for total phosphorus. In addition, the rate of CO2 removal from biogas was 63.38% ± 5.35%. Practitioner Points: The red and blue light intensity ratio of 5:5 showed the best removal performance.C. vulgaris‐G. lucidum‐S395‐2 system obtained the best photosynthetic performance.The carbonic anhydrase activity had positive effects on CO2 removal performance. [ABSTRACT FROM AUTHOR]

Summary: In this paper, the adaptive fuzzy controller design problem is investigated for a class of switched nonlinear systems in nonstrict feedback form, in which the unknown functions are considered and are approximated. Moreover, the system states are constrained in corresponding compact. By using Barrier Lypunov function method and backstepping technique, the adaptive fuzzy controller is designed such that all the signals in the closed‐loop system are bounded, the system output can track the desired signal to small compact, and all the system states satisfy the constraint conditions. Finally, the simulation results show the effectiveness of the proposed method. [ABSTRACT FROM AUTHOR]

Cardiac ischemia and reperfusion (IR) injury induces excessive emission of deleterious reactive O 2 and N 2 species (ROS/RNS), including the non-radical oxidant peroxynitrite (ONOO−) that can cause mitochondria dysfunction and cell death. In this study, we explored whether IR injury in isolated hearts induces tyrosine nitration of adenine nucleotide translocase (ANT) and alters its interaction with the voltage-dependent anion channel 1 (VDAC1). We found that IR injury induced tyrosine nitration of ANT and that exposure of isolated cardiac mitochondria to ONOO− induced ANT tyrosine, Y81, nitration. The exposure of isolated cardiac mitochondria to ONOO− also led ANT to form high molecular weight proteins and dissociation of ANT from VDAC1. We found that IR injury in isolated hearts, hypoxic injury in H9c2 cells, and ONOO− treatment of H9c2 cells and isolated mitochondria, each decreased mitochondrial bound-hexokinase II (HK II), which suggests that ONOO− caused HK II to dissociate from mitochondria. Moreover, we found that mitochondria exposed to ONOO− induced VDAC1 oligomerization which may decrease its binding with HK II. We have reported that ONOO− produced during cardiac IR injury induced tyrosine nitration of VDAC1, which resulted in conformational changes of the protein and increased channel conductance associated with compromised cardiac function on reperfusion. Thus, our results imply that ONOO− produced during IR injury and hypoxic stress impeded HK II association with VDAC1. ONOO− exposure nitrated mitochondrial proteins and also led to cytochrome c (cyt c) release from mitochondria. In addition, in isolated mitochondria exposed to ONOO− or obtained after IR, there was significant compromise in mitochondrial respiration and delayed repolarization of membrane potential during oxidative (ADP) phosphorylation. Taken together, ONOO− produced during cardiac IR injury can nitrate tyrosine residues of two key mitochondrial membrane proteins involved in bioenergetics and energy transfer to contribute to mitochondrial and cellular dysfunction. • Peroxynitrite (ONOO−) induces tyrosine nitration of ANT and VDAC1. • ONOO− cause dissociation of ANT from VDAC1, and HK II from VDAC1 • Protein dissociation enhances cytochrome c release from mitochondria. • ONOO− impairs mitochondrial bioenergetics, and compromises cardiac function after IR. [ABSTRACT FROM AUTHOR]

This paper applies uncertainty theory to the asset allocation problem for a defined contribution pension fund under a multi-period mean-variance framework. Different from most studies in the literature, both the security return and the salary are considered and assumed to be uncertain variables in this paper. The optimal portfolio adjustments are determined by minimizing the risk within the constraints of controlling the expected total incremental wealth with a minimum guarantee over the investment horizon. The transaction cost is also taken into account to simulate the real capital market. Finally, some special cases are discussed, and a numerical example is presented to illustrate the effectiveness of the model we proposed. [ABSTRACT FROM AUTHOR]

We provide evidence for location and function of a small conductance, Ca 2+ -activated K + (SK Ca ) channel isoform 3 (SK3) in mitochondria (m) of guinea pig, rat and human ventricular myocytes. SK Ca agonists protected isolated hearts and mitochondria against ischemia/reperfusion (IR) injury; SK Ca antagonists worsened IR injury. Intravenous infusion of a SK Ca channel agonist/antagonist, respectively, in intact rats was effective in reducing/enhancing regional infarct size induced by coronary artery occlusion. Localization of SK3 in mitochondria was evidenced by Western blot of inner mitochondrial membrane, immunocytochemical staining of cardiomyocytes, and immunogold labeling of isolated mitochondria. We identified a SK3 splice variant in guinea pig (SK3.1, aka SK3a) and human ventricular cells (SK3.2) by amplifying mRNA, and show mitochondrial expression in mouse atrial tumor cells (HL-1) by transfection with full length and truncated SK3.1 protein. We found that the N-terminus is not required for mitochondrial trafficking but the C-terminus beyond the Ca 2+ calmodulin binding domain is required for Ca 2+ sensing to induce mK + influx and/or promote mitochondrial localization. In isolated guinea pig mitochondria and in SK3 overexpressed HL-1 cells, mK + influx was driven by adding CaCl 2 . Moreover, there was a greater fall in membrane potential (ΔΨ m ), and enhanced cell death with simulated cell injury after silencing SK3.1 with siRNA. Although SK Ca channel opening protects the heart and mitochondria against IR injury, the mechanism for favorable bioenergetics effects resulting from SK Ca channel opening remains unclear. SK Ca channels could play an essential role in restraining cardiac mitochondria from inducing oxidative stress-induced injury resulting from mCa 2+ overload. [ABSTRACT FROM AUTHOR]

Aim: Excess mitochondrial reactive oxygen species (mROS) play a vital role in cardiac ischemia reperfusion (IR) injury. P66Shc, a splice variant of the ShcA adaptor protein family, enhances mROS production by oxidizing reduced cytochrome c to yield H2O2. Ablation of p66Shc protects against IR injury, but it is unknown if and when p66Shc is activated during cardiac ischemia and/or reperfusion and if attenuating complex I electron transfer or deactivating PKCβ alters p66Shc activation during IR is associated with cardioprotection. Methods: Isolated guinea pig hearts were perfused and subjected to increasing periods of ischemia and reperfusion with or without amobarbital, a complex I blocker, or hispidin, a PKCβ inhibitor. Phosphorylation of p66Shc at serine 36 and levels of p66Shc in mitochondria and cytosol were measured. Cardiac functional variables and redox states were monitored online before, during and after ischemia. Infarct size was assessed in some hearts after 120 min reperfusion. Results: Phosphorylation of p66Shc and its translocation into mitochondria increased during reperfusion after 20 and 30 min ischemia, but not during ischemia only, or during 5 or 10 min ischemia followed by 20 min reperfusion. Correspondingly, cytosolic p66Shc levels decreased during these ischemia and reperfusion periods. Amobarbital or hispidin reduced phosphorylation of p66Shc and its mitochondrial translocation induced by 30 min ischemia and 20 min reperfusion. Decreased phosphorylation of p66Shc by amobarbital or hispidin led to better functional recovery and less infarction during reperfusion. Conclusion: Our results show that IR activates p66Shc and that reversible blockade of electron transfer from complex I, or inhibition of PKCβ activation, decreases p66Shc activation and translocation and reduces IR damage. These observations support a novel potential therapeutic intervention against cardiac IR injury. [ABSTRACT FROM AUTHOR]

[Display omitted] • C-Br bond is activated on oxygen vacancies enriched Bi 2 WO 6. • Effective debromination is achieved on Bi 2 WO 6 under visible light irradiation. • Debromination on Bi 2 WO 6 follows a position-selective multi-electrons reduction mechanism. C Br bond activation is a critical step in the degradation of organic halogenated pollutants. Herein, by introducing oxygen vacancies with different concentration gradients into Bi 2 WO 6 , the CBr bond in decabromodiphenyl ether (BDE209) is activated, so that BDE209 is successfully dehalogenated under visible light. The study shows that the degradation rate of BDE209 on Bi 2 WO 6 is determined by both interface defects and crystallinity. Good crystallinity is conducive to the generation and separation of photogenerated electrons and holes, and an appropriate defect concentration can promote the C-Br activation of BDE209. In addition, through the tracking analysis of the degradation products of BDE209, the content of the meta -debromination products of BDE209 on Bi 2 WO 6 is significantly higher than that of the ortho- and para- debromination products, indicating that the reduction reaction is a position selective multi-electron mechanism. The study shows that oxygen vacancy assisted debromination is an excellent strategy for photocatalytic treatment of halogenated flame retardant pollutants. [ABSTRACT FROM AUTHOR]

Abstract: Hepatitis C virus (HCV) has a high rate of replication and lacks RNA-proofreading capabilities, thereby leading to variant or mutant viruses circulating within the host as a quasispecies. Previous work in our laboratory identified viral variants that emerged in a class-II immunodominant epitope NS3358-375 of the non-structural-3 (NS3) protein region of HCV, the sequence of which is based on genotype 1A, the most prevalent genotype in the United States. Further work suggested that positive immune selection pressure was driving viral variation. Paradoxically, viral variants account for only a small percentage of the circulating virus in human beings and in chimpanzees, suggesting that passive evasion is not the only means of escape by HCV. This observation suggests a unique pathogenesis for HCV as it persists in the host. In the current study, we hypothesize that viral variants are acting as altered peptide ligands (APLs). To test this hypothesis, we used cloned T cells specific for NS3358-375 peptide, which demonstrated attenuated T-cell and interferon-γ (IFN-γ) responses to individual variant peptides, when compared with the NS3358-375 stimulated T-cell clones. Furthermore, such variants could act as APLs, based on their ability to antagonize the IFN-γ proliferative responses of clones specific for NS3358-375. In addition, major histocompatibility complex (MHC) class II tetramer staining demonstrated that variant peptide–MHC complexes were able to specifically bind to NS3358-375 T-cell clones and that both the variant and NS3358-375 tetramers were able to bind to the same CD4+ T cells. Taken together, the results suggest that viral variants may act as APL to effectively blunt the T-cell response to an important HCV epitope. [Copyright &y& Elsevier]

Abstract: The mechanisms of action of gallium nitrate, an antineoplastic drug, are only partly understood. Using a DNA microarray to examine genes induced by gallium nitrate in CCRF-CEM cells, we found that gallium increased metallothionein-2A (MT2A) and heme oxygenase-1 (HO-1) gene expression and altered the levels of other stress-related genes. MT2A and HO-1 were increased after 6 and 16 h of incubation with gallium nitrate. An increase in oxidative stress, evidenced by a decrease in cellular GSH and GSH/GSSG ratio, and an increase in dichlorodihydrofluorescein (DCF) fluorescence, was seen after 1–4 h of incubation of cells with gallium nitrate. DCF fluorescence was blocked by the mitochondria-targeted antioxidant mitoquinone. N-Acetyl-L-cysteine blocked gallium-induced MT2A and HO-1 expression and increased gallium''s cytotoxicity. Studies with a zinc-specific fluoroprobe suggested that gallium produced an expansion of an intracellular labile zinc pool, suggesting an action of gallium on zinc homeostasis. Gallium nitrate increased the phosphorylation of p38 mitogen-activated protein kinase and activated Nrf-2, a regulator of HO-1 gene transcription. Gallium-induced Nrf-2 activation and HO-1 expression were diminished by a p38 MAP kinase inhibitor. We conclude that gallium nitrate induces cellular oxidative stress as an early event which then triggers the expression of HO-1 and MT2A through different pathways. [Copyright &y& Elsevier]

Bacterium strain PJ3, isolated from wastewater and identified as Arthrobacter sp. bacterium based on its 16S rDNA gene, could use carbazole as the sole carbon, nitrogen and energy source. The genomic library of strain PJ3 was constructed and a positive clone JM109 (pUCW402) was screened out for the expression of dioxygenase by the ability to form yellow ring-fission product. A 2,3-dihydroxybiphenyl dioxygenase (23DHBD) gene of 933 bp was found in the 3360 bp exogenous fragment of pUCW402 by GenSCAN software and BLAST analysis. The phylogenetic analysis showed that 23DHBD from strain PJ3 formed a deep branch separate from a cluster containing most known 23DHBD in GenBank. Southern hybridization confirmed for the first time that the 23DHBD gene was from the genomic DNA of Arthrobacter sp. PJ3. In order to test the gene function, recombinant bacterium BL21 (pETW-8) was constructed to express 23DHBD. The expression level in BL21 (pETW-8) was highest compared with the recombinant bacteria JM1O9 (pUCW402) and strain PJ3. We observed that 23DHBD was not absolute specific. The enzyme activity was higher with 2,3-dihydroxybiphenyl as a substrate than with catechol. The substrate specificity assay suggested that 23DHBD was essential for cleavage of bicyclic aromatic compounds during the course of aromatic compound biodegradation in Arthrobactersp. strain PJ3. [ABSTRACT FROM AUTHOR]

Abstract: The premise of this work is that within a given hepatitis C virus (HCV) protein there exists an array of Th1 and Th2 epitopes, each of which can provide synergistic (positive or negative) effects upon other epitopes by intramolecular, cytokine-mediated immunoregulation of helper T-cell responses. To address this question, we constructed minigene plasmids pHCVTh1, pHCVTh1X3 and pHCVThR, and HCV NS3 full-length plasmid pHCVNS3. 293T cells were transfected with these plasmids and cell lysates from the transfected cells were used to stimulate PBMC from a patient with chronic HCV infection. IL-2 and IFN-γ in the supernatant of the cultured PBMC were tested and proliferation of the PBMC was measured. The results demonstrate that interactions exist among helper T-cell epitopes; the synergistic effects of suppressive Th2 epitopes upon Th1 epitopes will inhibit the responses induced by Th1 epitopes, which may contribute to chronic infection by HCV; synergistic effects among Th1 epitopes induce higher levels of IFN-γ, which may suggest a new strategy for HCV vaccine development. Further, stimulation of an HCV NS3 specific clone with cell lysates from 293T cells transfected with different constructs shows that the HCV NS3 clone could respond to all suggesting that the epitope-specific suppression may be due to an imbalance of Type 1 and Type 2 cytokines or regulatory T-cells. [Copyright &y& Elsevier]

Abstract: Hepatitis C virus (HCV) causes non-A, non-B hepatitis and infects an estimated 170 million people worldwide. The treatment for HCV infection is often unsuccessful with high costs and many side-effects. There is a great need for alternative therapies including preventive and therapeutic vaccination for HCV infection. The experiments in this study were carried out to elucidate whether endogenously expressed antigen can be presented to helper T-cells restricted by class II molecules and to determine whether responses to plasmid-derived antigen resemble those that we have reported for recombinant antigens or synthetic peptides. To address these issues, a multi-epitope minigene was expressed in 293T-cells and Epstein-Barr virus (EBV)-transformed B-lymphoblastoid cells (BLCL). The transfected BLCLs were employed as APCs to stimulate epitope-specific T-cell hybridomas (THC). The results demonstrated that the endogenously expressed minigene antigens could be processed and presented to T-cell hybridomas by HLA matched BLCL. Five out of seven incorporated epitopes were recognized. Blockade of HLA DR could abolish the release of IL-2, which demonstrated that the endogenously expressed minigene antigens were presented by MHC class II molecules. The presentation of endogenously expressed antigens was much more efficient than that of exogenous antigens, at least in the present study. The findings obtained here have important significance for the development of an HCV DNA vaccine. [Copyright &y& Elsevier]

• C-Br activation by inducing oxygen vacancies on facet-dependent BiOCl. • The interface adsorption configuration determines the degradation path of BDE209. • The photocatalytic activity to BDE209 is completely different from RhB degradation. As typical persistent organic pollutants, polybrominated diphenyl ethers (PBDEs) have aroused high environmental concern due to their toxicity and recalcitrant degradation. Herein, the photocatalytic decabromodiphenyl ether (BDE209) removal performance was evaluated firstly on facet-dependent BiOCl with oxygen vacancies under irradiation (>360 nm). The results revealed that BDE209 was rapidly debrominated on {0 0 1} BiOCl with a reaction rate 4.4 times greater than that on {0 1 0} BiOCl under oxygen-free environment. Such a degradation order is completely opposite to that of Rhodamine B (RhB) degradation. This enhanced reduction is mainly attributed to the abundant oxygen vacancies on {0 0 1} facet of BiOCl, which act as adsorption sites for BDE209 to achieve C-Br bond activation efficiently. Meanwhile, debrominated products via meta- position have the highest proportion than that via ortho- and para- position in the first step. Therefore, a position-selective multi-electrons reduction mechanism is hypothesized. This study provides a new sight on C-X bond activation of halogenated aromatics by inducing oxygen vacancies on specific facets. [ABSTRACT FROM AUTHOR]

Investigates the genes related to the transformation of immortalized human fetal fibroblast cell by means of differential display messenger RNA (mRNA). Formation of clone on agar after irradiation; Number of DNA fragments; Analysis of mRNA expression.

We hypothesized that NO• is generated in isolated cardiac mitochondria as the source for ONOO− production during oxidative stress. We monitored generation of ONOO− from guinea pig isolated cardiac mitochondria subjected to excess Ca2+ uptake before adding succinate and determined if ONOO− production was dependent on a nitric oxide synthase (NOS) located in cardiac mitochondria (mtNOS). Mitochondria were suspended in experimental buffer at pH 7.15, and treated with CaCl 2 and then the complex II substrate Na-succinate, followed by menadione, a quinone redox cycler, to generate O 2 •−. L-tyrosine was added to the mitochondrial suspension where it is oxidized by ONOO− to form dityrosine (diTyr) in proportion to the ONOO− present. We found that exposing mitochondria to excess CaCl 2 before succinate resulted in an increase in diTyr and amplex red fluorescence (H 2 O 2) signals, indicating that mitochondrial oxidant stress, induced by elevated mtCa2+ and succinate, increased mitochondrial ONOO− production via NO• and O 2 •−. Changes in mitochondrial ONOO− production dependent on NOS were evidenced by using NOS inhibitors L-NAME/L-NNA, TEMPOL, a superoxide dismutase (SOD) mimetic, and PTIO, a potent global NO• scavenger. L-NAME and L-NNA decreased succinate and menadione-mediated ONOO− production, PTIO decreased production of ONOO−, and TEMPOL decreased ONOO− levels by converting more O 2 •− to H 2 O 2. Electron microscopy showed immuno-gold labeled iNOS and nNOS in mitochondria isolated from cardiomyocytes and heart tissue. Western blots demonstrated iNOS and nNOS bands in total heart tissue, bands for both iNOS and nNOS in β-tubulin-free non-purified (crude) mitochondrial preparations, and a prominent iNOS band, but no nNOS band, in purified (Golgi and ER-free) mitochondria. Prior treatment of guinea pigs with lipopolysacharride (LPS) enhanced expression of iNOS in liver mitochondria but not in heart mitochondria. Our results indicate that release of ONOO− into the buffer is dependent both on O 2 •− released from mitochondria and NO• derived from a mtCa2+-inducible nNOS isoform, possibly attached to mitochondria, and a mtNOS isoform like iNOS that is non-inducible. Unlabelled Image • Cardiac isolated mitochondria produce and release ONOO−, a product of O 2 •− and NO•. • O 2 •− is generated by complex I during oxidative stress induced by excess CaCl 2 and succinate. • NO• is generated within or near isolated mitochondria. • During oxidative stress mitochondrial ONOO− levels are modulated by O 2 •− and NO•. • Mitochondrial NOS is likely a variant of a non-inducible iNOS, but a nNOS may also contribute. [ABSTRACT FROM AUTHOR]

BACKGROUND: Dry cultivation of rice is a water‐saving, emission reduction and labor‐saving rice farming method. However, the development of rice under dry cultivation is hampered by the limitations of dry cultivation on rice yield and rice quality. We hypothesized that additional silicon (Si) would be a measure to address these limitations or challenges. RESULTS: In the present study, we set up field trials with three treatments: flooded cultivation (W), dry cultivation (D) and dry cultivation plus Si. Yield and quality were reduced under D treatment compared to W treatment. The addition of Si promoted root development, increased plant height and leaf area, increased photosynthetic enzyme activity, net photosynthetic rate and SPAD values, and increased biomass under dry crop conditions. Under the drought conditions, silica up‐regulated the expression of AGPSI, SBEI, SBEIIb, SSI and SSII‐1 genes and the activities of ADP‐glucose pyrophosphorylase (AGPase), soluble starch synthetase (SSS) and starch branching enzyme (SBE) enzymes, which reduced protein, amylose, chalkiness percentage and chalkiness degree, increased brown rice rate, milled rice rate and head milled rice rate, and also improved rice quality. In addition, the increase of AGPase, SSS and SBE enzyme activities promoted the filling rate and the number of spikes was guaranteed, whereas the yield was improved by promoting the seed setting rate and 1000‐grain weight. CONCLUSION: The results of the present study indicate that adding appropriate amounts of Si fertilizer can improve the yield and quality of rice under dry cultivation by regulating source supply capacity and grain starch synthesis. © 2023 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Glutathione transferases (GSTs) are a superfamily of dimeric proteins associated with the detoxification of various reactive electrophiles and responsive to a multitude of stressors. We individually substituted Lys64 and Glu78 with Ala using site-directed mutagenesis to understand the role of subunit interactions in the structure and enzymatic properties of a rice GST (OsGSTU17). The wild-type OsGSTU17 lost the conserved hydrogen bond between subunits in tau class GSTs due to conserved Tyr92 replaced with Phe92, but still exhibited high substrate activities, and thermal stability remained in its dimeric structure. The significant decrease in thermal stability and obvious changes in the structure of mutant K64A implied that conserved Lys64 might play an essential role in the structural stability of tau class GSTs. The mutant E78A, supposed to be deprived of hydrogen and salt bonds between subunits, appeared in the soluble form of dimers, even though its tertiary structure altered and stability declined dramatically. These results suggest that the hydrogen and ionic bonds provided by conserved residues are not as important for OsGSTU17 dimerization and enzymatic properties. These results further supplement our understanding of the relationship between the structure and function of GSTs and provide a theoretical basis for improving crop resistance through targeted modification of GSTs. [ABSTRACT FROM AUTHOR]

The conflict between rice production and water scarcity is becoming more pronounced. Therefore, the advancement of water‐saving rice cultivation is crucial in guaranteeing both food and ecological security. This study provides a summary of the development and characteristics of various water‐saving cultivation techniques for rice, including alternate wetting and drying irrigation, wet irrigation, controlled irrigation, the system of rice intensification, direct seeding rice, and dry cultivation of rice (DCR). We also introduce the varietal characteristics of upland rice, aerobic rice, water‐saving and drought‐resistant rice, and DCR. We summarize the impact of different water‐saving cultivation models on yield and quality and regulatory measures, and propose challenges and strategies for the development of water‐saving cultivation of rice in the future. We hope to provide a reference for promoting the development of rice water‐saving cultivation and dry farming agriculture. Core Ideas: This review summarizes the development of water‐saving cultivation techniques for rice.This review presents the challenges and strategies for developing water‐saving rice cultivation.This review provides technical and theoretical support for the development of rice water‐saving cultivation. [ABSTRACT FROM AUTHOR]

In view of the importance of inorganic phosphate to plant growth and development, the role of phosphate transporters responsible for absorption and transportation in crops has attracted increasing attention. In this study, bioinformatics analysis and subcellular localisation experiment showed that GmPHT4;10 is a member of PHT4 subfamily of phosphate transporters and located in chloroplasts. The gene was induced by phosphate deficiency and drought, and was the highest in leaves. After GmPHT4;10 gene was replenished to AtPHT4;5 gene deletion mutant lines (atpht4;5), the phenotype of the transgenic lines was basically recovered to the level of wild-type, but there were significant differences in phosphate content and photosynthetic indicators between wild-type and revertant lines. Meanwhile, the difference of proline content and catalase activity between the two lines also indicated that GmPHT4;10 gene and its orthologous gene AtPHT4;5 were different in drought resistance and drought resistance mechanism. After overexpression of GmPHT4;10 gene in Arabidopsis thaliana , more phosphate and proline were accumulated in chloroplasts and catalase activity was increased, thus improving photosynthesis and drought resistance of plants. The results further supplement the cognition of PHT4 subfamily function, and provides new ideas and ways to improve photosynthesis by revealing the function of chloroplast phosphate transporter. GmPHT4;10 gene is a member of PHT4 subfamily of soybean (Glycine max) phosphate transporter gene family. GmPHT4;10 gene expression was induced by drought and low phosphorus stress. GmPHT4;10 gene has the physiological function of regulating chloroplast phosphorus content and improving plant drought resistance, but there are differences between GmPHT4;10 gene and its orthologous gene AtPHT4;5 in drought resistance mechanism. [ABSTRACT FROM AUTHOR]

Phosphorus is an essential macronutrient for plant growth and development, but phosphate resources are limited and rapidly depleting due to massive global agricultural demand. This study identified two genes in the phosphate transporter 2 (PHT2) family of soybean by bioinformatics. The expression patterns of two genes by qRT-PCR at leaves and all were induced by low-phosphate stress. After low-phosphate stress, GmPHT2;2 expression was significantly higher than GmPHT2;1, and the same trend was observed throughout the reproductive period. The result of heterologous expression of GmPHT2 in Arabidopsis knockout mutants of atpht2;1 shows that chloroplasts and whole-plant phosphorus content were significantly higher in plants complementation of GmPHT2;2 than in plants complementation of GmPHT2;1. This suggests that GmPHT2;2 may play a more important role in plant phosphorus metabolic homeostasis during low-phosphate stress than GmPHT2;1. In the yeast backfill assay, both genes were able to backfill the ability of the defective yeast to utilize phosphorus. GmPHT2 expression was up-regulated by a low-temperature treatment at 4 °C, implying that GmPHT2;1 may play a role in soybean response to low-temperature stress, in addition to being involved in phosphorus transport processes. GmPHT2;1 and GmPHT2;2 exhibit a cyclic pattern of circadian variation in response to light, with the same pattern of gene expression changes under red, blue, and white light conditions. GmPHT2 protein was found in the chloroplast, according to subcellular localization analysis. We conclude that GmPHT2 is a typical phosphate transporter gene that can improve plant acquisition efficiency. [ABSTRACT FROM AUTHOR]

High grain yields of rice (Oryza sativa) under dry cultivation are primarily obtained through high levels of nitrogen (N) input. However, excessive inputs of N increase the risk of lodging. This study aimed to clarify the effect of N application rates on crop morphology, mechanical mechanisms of the stem, and chemical components in the basal stems of rice and their underlying mechanism in association with lodging resistance under dry cultivation. In this study, field experiments on rice were conducted under dry cultivation in early May to early October 2019 and 2020. Six rates of N applied at 0, 70, 140, 210, 280 and 350 kg ha−1 were set at a sowing rate of 150 kg ha−1 with Suijing 18 as the test material. The increased risk of lodging represented by lodging index (LI) and lodging rate with increasing N application was observed under both growing seasons. The plant height, basal internode length, particularly the second internode, and center of gravity height, which positively correlated with the LI, increased significantly with the increase in N application rates. In contrast, internode fullness and carbohydrate content of the basal second (S2) internode, which negatively correlated with LI, decreased significantly with increasing N application rates. A quadratic regression model between N application rates and grain yield showed that better grain yield could be achieved under an N application rate ranging from 210 to 228 kg ha−1. Therefore, the N application rate of (i.e., 210–228 kg N ha−1) could be recommended for the Suijing18 variety under dry cultivation in central Jilin Province for achieving high grain yield and great lodging resistance. [ABSTRACT FROM AUTHOR]

The family of phosphate transporters (PHTs) mediates the uptake and translocation of Pi inside the plants. However, little is known about transporters in soybean. Therefore, Searched the Genome Database for Soybean, 57 GmPHTs family members were identified in soybean, Phylogenetic analysis suggested that members of the PHTs gene family can be divided into six clades. Collinearity analysis revealed that most of the GmPHT genes shared syntenic relationships with PHTs members in Arabidopsis thaliana and that large segment duplication played a major driving force for GmPHTs evolution in addition to tandem duplication. Further analysis of the promoter revealed that light-responsive elements and abiotic stress-responsive elements were widely distributed within the promoter regions of GmPHT genes. Based on RNA-seq data, GmPHTs showed different expression patterns in roots and leaves of soybean treated with long-term low phosphorus and short-term low phosphorus, in addition, the expression levels of GmPHT genes can be regulated by drought stresses, it was implied that the induced expression of GmPHTs could promote phosphorus uptake and transport in soybean and thus adapt to low phosphorus and drought stress, which is the first step dissection of Pi transport system and probably refers to new roles of PHTs genes in soybean. [ABSTRACT FROM AUTHOR]

In order to explore the distribution of cyanobacteria in paddy soil under different ecological conditions, the composition, diversity, and environmental drivers of soil cyanobacteria communities in rice fields from six regions of Jilin Province (China) were investigated. The results showed that the 16S amplicon high-throughput sequence detected the existence of cyanobacteria of 16 phyla, 33 orders, 60 families, and 113 genera in the soil of rice fields in Jilin Province. The dominant cyanobacteria populations in Jilin Province paddy soils comprised Cyanobium_PCC-6307, Synechocystis_PCC-6803, Planktothrix_NIVA-CYA_15, and Nodosilinea_PCC-7104. Each soil sample included a significant proportion of nitrogen-fixing filamentous cyanobacteria Anabaena and Nostoc according to microscopic analysis. The structural properties and diversity of cyanobacteria communities differed by geography, with soil pH and SOC being the main environmental drivers of cyanobacteria community structure. The alkaline soils S1, S2, and S5 displayed greater diversity than the acidic soils S3, S4, and S6, with S5 displaying the greatest cyanobacteria diversity. This research has crucial implications for developing and utilizing local cyanobacteria resources. [ABSTRACT FROM AUTHOR]

This study was aimed to clarify the effects of the interaction between water and nitrogen on grain yield and greenhouse gases (GHGs) emissions under straw incorporation (SI) in a rice paddy field in northeast China. Under standard local rice paddy fields conditions, a split-plot design was used, with continuous flooding (CF) irrigation and alternate wetting and drying (AWD) irrigation as primary plot treatments, and traditional nitrogen application management (TNAM, basal fertilizer:tiller fertilizer:panicle fertilizer:spikelet fertilizer, 6:3:1:0), postponed nitrogen topdressing management 1 (PNTM1, basal fertilizer:tiller fertilizer:panicle fertilizer:spikelet fertilizer, 5:2:2:1), and postponed nitrogen topdressing management 2 (PNTM2, basal fertilizer:tiller fertilizer:panicle fertilizer:spikelet fertilizer, 4:3:2:1) as secondary plot treatments. The cumulative GHGs emissions, global warming potential (GWP), and grain yield at each reproductive stage under interactions between water and nitrogen were examined. Compared with CF, the grain yield of AWD in 2018 and 2019 increased significantly by 8.84% and 9.93%, respectively. There was no significant difference in grain yield between different nitrogen management practices under the same irrigation pattern. Compared with CF, AWD reduced CO2 and CH4 emissions by 27.82% and 47.96%, increased N2O emissions by 18.78%, and reduced the GWP and greenhouse gas intensity (GHGI) by 38.27% and 44.18%. Under AWD, there were no significant differences in CH4 emissions and GHGI between different nitrogen management treatments, and PNTM1 and PNTM2 significantly reduced CO2 emissions by 36.55% and 22.99%, increased N2O emissions by 70.59% and 76.47%, and significantly reduced GWP by 29.51% and 19.73%, respectively. Compared to TNAM, with PNTM1 achieving better emission reduction than PNTM2. Thus, AWD with PNTM1 under SI had the most significant abatement effect with stable grain yields. Thus, emission reductions can be achieved through utilizing the interaction between water and nitrogen under SI to ensure sustainable rice production in agricultural areas like those of central Jilin province. [ABSTRACT FROM AUTHOR]

In the present study, 27 bacterial strains were isolated from environmental samples and screened for higher lignocellulose-degrading efficiency. The best degrader was combined in pairs with 14 strains with high β-glucosidase activity to formulate a consortium. Microbial consortium 625 showed high lignocellulose degradation efficiency. ZJW-6 with low β-glucosidase activity and the best lignocellulose decomposer was identified as a member of Cellulomonas. Consortium 625 composed of ZJW-6 and DA-25, an Acinetobacter, showed the highest degradation rate (57.62%) under optimized conditions. The DA-25 filtrate promoted ZJW-6 growth, upregulating the activity of key lignocellulose-degrading enzymes, including β-glucosidase, endoglucanase, xylanase, laccase, and lignin peroxidase. ZJW-6 and DA-25 worked in a subordination manner when co-cultivated. ZJW-6 acted as the major decomposer whose growth and enzymatic activities were promoted by DA-25. This study proposes a novel microbial consortium with improved lignocellulose degradation efficiency and reduce the C:N ratio of lignocellulose materials, which can enhance bioenergy production. [ABSTRACT FROM AUTHOR]

Complex formation between hexokinase-II (HKII) and the mitochondrial VDAC1 is crucial to cell growth and survival. We hypothesize that HKII first inserts into the outer membrane of mitochondria (OMM) and then interacts with VDAC1 on the cytosolic leaflet of OMM to form a binary complex. To systematically investigate this process, we devised a hybrid approach. First, we describe membrane binding of HKII with molecular dynamics (MD) simulations employing a membrane mimetic model with enhanced lipid diffusion capturing membrane insertion of its H-anchor. The insertion depth of the H-anchor was then used to derive positional restraints in subsequent millisecond-scale Brownian dynamics (BD) simulations to preserve the membrane-bound pose of HKII during the formation of the HKII/VDAC1 binary complex. Multiple BD-derived structural models for the complex were further refined and their structural stability probed with additional MD simulations, resulting in one stable complex. A major feature in the complex is the partial (not complete) blockade of VDAC1's permeation pathway, a result supported by our comparative electrophysiological measurements of the channel in the presence and absence of HKII. We also show how VDAC1 phosphorylation disrupts HKII binding, a feature that is verified by our electrophysiology recordings and has implications in mitochondria-mediated cell death. Haloi et al. investigate the role of the hydrophobic N-terminus of hexokinase II (HKII) in their binding to mitochondrial VDAC1. They use a hybrid approach combining molecular and Brownian dynamics to show that HKII first inserts into the outer membrane of mitochondria (OMM) and then interacts with VDAC1 on the cytosolic surface of OMM to form a binary complex. [ABSTRACT FROM AUTHOR]

Lung cancer is the most common cancer type worldwide and the leading cause of cancer-related mortality. Diabetes is closely associated with the occurrence, development and prognosis of lung cancer. Therefore, the present study aimed to investigate whether SNCG could affect the proliferation of lung cancer cells induced by high glucose. Lung cancer cells induced by high glucose simulated the pathologies of patients with lung cancer with diabetes in vitro. The proliferation of HBE cells and lung cancer cells after transfection and treatment of glucose was detected using Cell Counting Kit-8 assay. The mRNA expression levels of synuclein γ (SNCG), insulin-like growth factor 1 (IGF-1) and IGF-1 receptor (IGF-1R) in HBE cells and lung cancer cells alone, or cells induced by high glucose were analyzed via reverse transcription-quantitative (RT-q)PCR analysis. Moreover RT-qPCR analysis was used to determine the transfection efficiencies. The clone formation ability, migration and inflammation of lung cancer cells after high glucose induction and transfection were detected using clone formation, wound healing and ELISA assays. The protein expression levels of SNCG, IGF-1, IGF-1R, ERK 1/2, phosphorylated (p)-ERK1/2 and JNK in lung cancer cells after high glucose induction and transfection were determined using western blot analysis. The results suggested that high glucose significantly promoted the proliferation of A549, NCI-H1975 and SK-MES-1 cells at 24 and 48 h, as well as upregulated the expression levels of SNCG, IGF-1 and IGF-1R. Knockdown of SNCG suppressed the proliferation, clone formation ability and migration, but alleviated inflammation in A549 cells induced by high glucose. Knockdown of SNCG suppressed the expression levels of SNCG, IGF-1, IGF-1R, ERK1/2 and p-ERK1/2, while it promoted JNK expression in A549 cells induced by high glucose. The effect of AXL1717 (an IGF-1R inhibitor) treatment on cells was consistent with that of SNCG knockdown. In conclusion, inhibition of SNCG suppresses proliferation of lung cancer cells induced by high glucose. [ABSTRACT FROM AUTHOR]

• Amylase activity were lower after drought treatment than during flood cultivation. • The expression levels of related amylase genes in dry cultivation were different during grain filling. • Key period for quality formation of dry cultivation was from 8DAF to 15DAF. • At 8–15DAF, accumulation of metabolites in dry cultivation rice was higher than controls. • At 40DAF, metabolite consumption of dry cultivation rice was faster than control. The differences between dry- and flood-cultivated rice and the reason behind low-quality dry-cultivated rice were clarified. The physiological traits, starch synthase activity, and grain metabolomics of 'Longdao 18′ were measured and analyzed at four growth stages. The brown, milled, and whole-milled rice rates and AGPase , SSS , and SBE activity were lower after drought treatment than during flood cultivation, while the chalkiness, chalky grain rate, amylose (16.57–20.999%), protein (7.99–12.09%), and GBSS activity were higher. Related enzymatic gene expression showed significant differences. Metabolic results showed pyruvate, glycine, and methionine upregulation at 8DAF and higher citric, pyruvic, and α-ketoglutaric acid content at 15DAF. Therefore, 8DAF-15DAF represented the crucial quality formation period for dry-cultivated rice. At 8DAF, the respiratory pathways used amino acids as signaling molecules and alternative substrates to adapt to energy shortages, arid environments and rapid protein accumulation and synthesis. Excessive amylose synthesis at 15DAF accelerated reproductive growth, promoting rapid premature aging. [ABSTRACT FROM AUTHOR]

CAZymes play a very important role in the biotransformation of corn stalk biomass, which is an important resource for sustainable development. Pleurotus djamor can produce CAZymes related to the decomposition of corn stalk lignocellulose biomass in sole corn stalk substrate; however, little is known about their encoding genes. In order to identify CAZymes encoding genes, RNA high-throughput sequencing of P. djamor was performed in this study. The results showed that a core set of 70 upregulated genes encoding putative CAZymes were revealed. They encode 19 kinds of CAZymes in total, of which there are 4 EGLs, 8 CBHs, 5 BGLs, and 12 LPMOs related to cellulose degradation, 8 XYNs, 1 XYL, 2 AGUs, 3 ABFs, 2 AGLs, and 2 AXEs related to hemicellulose degradation, and 5 LACCs, 2 MnPs, 5 VPs, 3 CDHs, 1 AAO, 1 GOX, 1 AOX, 2 GAOXs, and 3 GLOXs related to lignin degradation. This variety suggests that CAZymes may play a very important role in decomposing the lignocellulose biomass of corn stalk. This is the first study to report the de novo transcriptome sequencing of P. djamor , which will produce a dataset of genes encoding CAZymes, thereby laying the foundation to elucidate the degradation mechanism of corn stalk biomass and boost the biotransformation of corn stalk biomass resources. [ABSTRACT FROM AUTHOR]

Contradictory reports on the effects of diabetes and hyperglycemia on myocardial infarction range from cytotoxicity to cytoprotection. The study was designed to investigate acute effects of high glucose-driven changes in mitochondrial metabolism and osmolarity on adaptive mechanisms and resistance to oxidative stress of isolated rat cardiomyocytes. We examined the effects of high glucose on several parameters of mitochondrial bioenergetics, including changes in oxygen consumption, mitochondrial membrane potential, and NAD(P)H fluorometry. Effects of high glucose on the endogenous cytoprotective mechanisms elicited by anesthetic preconditioning (APC) and the mediators of cell injury were also tested. These experiments included real-time measurements of reactive oxygen species (ROS) production and mitochondrial permeability transition pore (mPTP) opening in single cells by laser scanning fluorescence confocal microscopy, and cell survival assay. High glucose rapidly enhanced mitochondrial energy metabolism, observed by increase in NAD(P)H fluorescence intensity, oxygen consumption, and mitochondrial membrane potential. This substantially elevated production of ROS, accelerated opening of the mPTP, and decreased survival of cells exposed to oxidative stress. Abrogation of high glucose-induced mitochondrial hyperpolarization with 2,4 dinitrophenol (DNP) significantly, but not completely, attenuated ROS production to a level similar to hyperosmotic mannitol control. DNP treatment reversed high glucose-induced cytotoxicity to cytoprotection. Hyperosmotic mannitol treatment also induced cytoprotection. High glucose abrogated APC-induced mitochondrial depolarization, delay in mPTP opening and cytoprotection. In conclusion, high glucose-induced mitochondrial hyperpolarization abolishes APC and augments cell injury. Attenuation of high glucose-induced ROS production by eliminating mitochondrial hyperpolarization protects cardiomyocytes. J. Cell. Physiol. 232: 216-224, 2017. © 2016 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]

[Objectives]In order to clear and define the species of phosphate-solubilizing bacteria(PSB)strains from the black soil of soybean rhizosphere,the characteristics of dissolving inorganic phosphorus of different PSB and maintain the sustainable utilization ability of the black soil by using PSB. [Methods]Strain bacterium wj6 was isolated from rhizosphere soil of soybean by NBRIP medium. The Vitek 2 automatic analysis system and 16S rDNA sequences technology were used to identify the bacterium;on the basis of NBRIP medium,the orthogonal design L25(53)was employed in decision medium optimization from the carbon source,nitrogen source and pH value for strain wj6 phosphorus solubilization;the dissolving characteristics of Ca3(PO4)2,FePO4,AlPO4 and phosphatase activity and glucose dehydrogenase activity in optimized NBRIP medium were determined. [Results]The results indicated that the effective PSB wj6 was screened and identified as Klebsiella sp.. The highest capacity of solubilizing phosphate appeared when strain wj6 was supplied with glucose and peptone as carbon source and nitrogen source and the medium pH value was 8. The strain wj6 exhibited higher solubilizing abilities in Ca3(PO4)2,FePO4,AlPO4 as sole source of phosphorus,and maximum amount of phosphorus solubilization were 409.28,60.59,and 32.90 μg.mL-1,respectively. Furthermore,the solubilizing phosphorus ability of strain wj6 was strongly influenced by environmental pH value when FePO4 and AlPO4 were the phosphorus source. The strain wj6 could dissolve FePO4 and AlPO4 when it was inoculated to soluble phosphorus medium(pH 8). However,no phosphorus solubilizing was found in the medium(pH 3). With the use of Ca3(PO4)2 as the sole source of phosphorus,the activity of acid phosphatase,alkaline phosphatase,and glucose dehydrogenase showed the reduction after the first increase in the process of solubilizing phosphorus. Glucose dehydrogenase activity was larger in 36 h than that of other times and showed the significant differences. [Conclusions]Klebsiella sp.wj6 isolated from soybean rhizosphere showed the significant difference in solubilizing Ca3(PO4)2,FePO4 and AlPO4,and phosphorus solubilization was influenced by environmental pH value. Phosphatase and glucose dehydrogenase played an important role in the process of solubilizing phosphorus. [ABSTRACT FROM AUTHOR]

Leymus chinensis (Trin.) Tzvel. is a perennial rhizome grass of the Poaceae (also called Gramineae) family, which adapts well to drought, saline and alkaline conditions. However, little is known about the stress tolerance of L. chinensis at the molecular level. microRNAs (miRNAs) are known to play critical roles in nutrient homeostasis, developmental processes, pathogen responses, and abiotic stress in plants. In this study, we used Solexa sequencing technology to generate high-quality small RNA data from three L. chinensis groups: a control group, a saline-alkaline stress group (100 mM NaCl and 200 mM NaHCO3), and a drought stress group (20% polyethylene glycol 2000). From these data we identified 132 known miRNAs and 16 novel miRNAs candidates. For these miRNAs we also identified target genes that encode a broad range of proteins that may be correlated with abiotic stress regulation. This is the first study to demonstrate differentially expressed miRNAs in L. chinensis under saline-alkali and drought stress. These findings may help explain the saline-alkaline and drought stress responses in L. chinensis. [ABSTRACT FROM AUTHOR]

Abstract: Ranolazine, an anti-anginal drug, is a late Na+ channel current blocker that is also believed to attenuate fatty acid oxidation and mitochondrial respiratory complex I activity, especially during ischemia. In this study, we investigated if ranolazine''s protective effect against cardiac ischemia/reperfusion (IR) injury is mediated at the mitochondrial level and specifically if respiratory complex I (NADH Ubiquinone oxidoreductase) function is protected. We treated isolated and perfused guinea pig hearts with ranolazine just before 30min ischemia and then isolated cardiac mitochondria at the end of 30min ischemia and/or 30min ischemia followed by 10min reperfusion. We utilized spectrophotometric and histochemical techniques to assay complex I activity, Western blot analysis for complex I subunit NDUFA9, electron paramagnetic resonance for activity of complex I Fe–S clusters, enzyme linked immuno sorbent assay (ELISA) for determination of protein acetylation, native gel histochemical staining for respiratory supercomplex assemblies, and high pressure liquid chromatography for cardiolipin integrity; cardiac function was measured during IR. Ranolazine treated hearts showed higher complex I activity and greater detectable complex I protein levels compared to untreated IR hearts. Ranolazine treatment also led to more normalized electron transfer via Fe–S centers, supercomplex assembly and cardiolipin integrity. These improvements in complex I structure and function with ranolazine were associated with improved cardiac function after IR. However, these protective effects of ranolazine are not mediated by a direct action on mitochondria, but rather indirectly via cytosolic mechanisms that lead to less oxidation and better structural integrity of complex I. [Copyright &y& Elsevier]

Abstract: Ranolazine is a clinically approved drug for treating cardiac ventricular dysrhythmias and angina. Its mechanism(s) of protection is not clearly understood but evidence points to blocking the late Na+ current that arises during ischemia, blocking mitochondrial complex I activity, or modulating mitochondrial metabolism. Here we tested the effect of ranolazine treatment before ischemia at the mitochondrial level in intact isolated hearts and in mitochondria isolated from hearts at different times of reperfusion. Left ventricular (LV) pressure (LVP), coronary flow (CF), and O2 metabolism were measured in guinea pig isolated hearts perfused with Krebs-Ringer''s solution; mitochondrial (m) superoxide (O2 −), Ca2+, NADH/FAD (redox state), and cytosolic (c) Ca2+ were assessed on-line in the LV free wall by fluorescence spectrophotometry. Ranolazine (5μM), infused for 1min just before 30min of global ischemia, itself did not change O2 −, cCa2+, mCa2+ or redox state. During late ischemia and reperfusion (IR) O2 − emission and m[Ca2+] increased less in the ranolazine group vs. the control group. Ranolazine decreased c[Ca2+] only during ischemia while NADH and FAD were not different during IR in the ranolazine vs. control groups. Throughout reperfusion LVP and CF were higher, and ventricular fibrillation was less frequent. Infarct size was smaller in the ranolazine group than in the control group. Mitochondria isolated from ranolazine-treated hearts had mild resistance to permeability transition pore (mPTP) opening and less cytochrome c release than control hearts. Ranolazine may provide functional protection of the heart during IR injury by reducing cCa2+ and mCa2+ loading secondary to its effect to block the late Na+ current. Subsequently it indirectly reduces O2 − emission, preserves bioenergetics, delays mPTP opening, and restricts loss of cytochrome c, thereby reducing necrosis and apoptosis. [Copyright &y& Elsevier]

We used a rat model of whole thorax x-ray irradiation to profile the microRNA (miRNA) in lung and blood up to 4 weeks after radiation. MiRNA from normal and irradiated Wistar rat lungs and whole blood were analyzed by next-generation sequencing and the changes by radiation were identified by differential deRNA-seq 1, 2, 3 and 4 weeks after irradiation. The average total reads/library was 2,703,137 with a mean of 88% mapping to the rat genome. Detailed profiles of 100 of the most abundant miRNA in rat blood and lung are described. We identified upregulation of 4 miRNA, miR-144-5p, miR-144-3p, miR-142-5p and miR-19a-3p in rat blood 2 weeks after radiation that have not previously been shown to be altered after radiation to the lung. Ingenuity Pathway Analysis identified signaling of inflammatory response pathways. These findings will support development of early detection methods, as well as mechanism(s) of injury and mitigation in patients after radiotherapy or radiological accidents. [ABSTRACT FROM AUTHOR]