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Organoids have attracted extensive attention in the field of hair follicle (HF) tissue engineering and regeneration. However, HF organoid implantation faces a number of difficulties, such as low viability ratios, invasive trauma, and complicated procedures. To overcome these issues, a convenient and minimally invasive delivery approach with high efficiency and cell viability is required. Here, HF organoid‐loaded methacrylated gelatin‐cryomicroneedles (GelMA‐cryoMNs) that are capable of efficient transdermal delivery and can maintain the viability of the embedded organoids are developed. The GelMA‐cryoMNs are fabricated by a stepwise cryogenic molding process using a balanced ratio of cryopreservation agents and GelMA hydrogel. They can be inserted easily into porcine skin and melted without leaving residue after delivering the organoids. In vitro and in vivo, organoids delivered by the cryoMNs retain cell viability, self‐assembly, and differentiation ability. In mice, HF organoid‐loaded GelMA‐cryoMNs are delivered to the superficial dermis, resulting in subsequent HF development and the formation of an array of biomimetic hair tufts that broke through the skin surface. A promising strategy is provided here for biomimetic hair neogeneration that benefits from minimal invasion, low cost, and simple fabrication. The GelMA‐cryoMNs developed here could also have broader applications in tissue engineering and organ regeneration. [ABSTRACT FROM AUTHOR]

Androgenetic alopecia is the most common form of hair loss disorder. The features of this process are shortening of the anagen phase in hair cycling and progressive miniaturization of the hair follicle. However, the mechanisms in androgenetic alopecia are still unclear, and the treatment methods are also limited. Therefore, further study on the pathogenesis and new therapies for androgenetic alopecia are urgently needed. In this study, we found that endogenous autophagy was severely impaired, accompanied by increased apoptosis in early catagen‐like miniaturized hair follicles from the balding scalps of androgenetic alopecia patients. Moreover, inhibition of autophagy using 3‐methyladenine could induce apoptosis, premature hair follicle regression and slow down the hair growth in organ‐cultured hair follicles. Taken together, these results suggest that impairment of autophagy could be a potential mechanism in androgenetic alopecia. [ABSTRACT FROM AUTHOR]

Intracutaneous transplantation of trichogenic cells is a currently endorsed strategy to realize hair regeneration in vivo. However, skin is not the most advantageous transplant site due to the robust mechanical property and deficient physiological perfusion. Herein, a subcutaneous space made of prevascularized collagen fibers (PVCF) generated by controlling the duration of in situ foreign body reaction is reported. In contrast to skin, an optimally preprogrammed PVCF presents a larger tissue volume (171 mm3), low Young's modulus (1/2‐fold), high flexibility high mechanical energy dissipation rate (1.3‐fold), highly permeable surface structure, and plentiful vascular network. Remarkably, cells transplanted into PVCF suffer less apoptosis and necrosis, and generate more mature hairs (≈289 per site) than that in intracutaneous transplantation (≈177 per site). The RNA‐sequencing profiling indicates similar trends under molecular level. This work provides a promising strategy to improve graft site microenvironment physiologically and mechanically, with a low‐cost and simple fabrication process. [ABSTRACT FROM AUTHOR]

Purpose: To investigate the therapeutic efficiency of a novel drink termed "Ferment" in cases of ulcerative colitis (UC) and its influence on the gut microbiota., Method: In this study, we developed a complex of mixed fruit juice and lactic acid bacteria referred to as Ferment. Ferment was fed to mice for 35 days, before inducing UC with Dextran Sulfate Sodium Salt. We subsequently investigated the gut microbiome composition using 16S rRNA sequencing., Result: After Ferment treatment, mouse body weight increased, and animals displayed less diarrhea, reduced frequency of bloody stools, and reduced inflammation in the colon. Beneficial bacteria belonging to Ileibacterium , Akkermansia , and Prevotellacea were enriched in the gut after Ferment treatment, while detrimental organisms including Erysipelatoclostridium , Dubosiella , and Alistipes were reduced., Conclusion: These data place Ferment as a promising dietary candidate for enhancing immunity and protecting against UC., Competing Interests: SW and QB was employed by Junlebao Dairy Group Co., Ltd. GW was employed by Jiangsu Biodep Biotechnology Co., Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Chen, Ye, Shi, Wang, Deng, Yuan, Wang, Wang, Yang and Mou.)