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Traffic shockwaves demonstrate the formation and spreading of traffic fluctuation on roads. Existing methods mainly detect the shockwaves and their propagation by estimating traffic density and flow, which presents weaknesses in applications when traffic data is only partially or locally collected. This paper proposed a four-step data-driven approach that integrates machine learning with the traffic features to detect shockwaves and estimate their propagation speeds only using partial vehicle trajectory data. Specifically, we first denoise the speed data derived from trajectory data by the Fast Fourier Transform (FFT) to mitigate the effect of spontaneous random speed fluctuation. Next, we identify trajectory curves' turning points where a vehicle runs into a shockwave and its speed presents a high standard deviation within a short interval. Furthermore, the Density-based Spatial Clustering of Applications with Noise algorithm (DBSCAN) combined with traffic flow features is adopted to split the turning points into different clusters, each corresponding to a shockwave with constant speed. Last, the one-norm distance regression method is used to estimate the propagation speed of detected shockwaves. The proposed framework was applied to the field data collected from the I-80 and US-101 freeway by the Next Generation Simulation (NGSIM) program. The results show that this four-step data-driven method could efficiently detect the shockwaves and their propagation speeds without estimating the traffic densities and flows nearby. It performs well for both homogenous and nonhomogeneous road segments with trajectory data collected from total or partial traffic flow. [ABSTRACT FROM AUTHOR]

Rhododendron, a globally popular ornamental flower, is nevertheless limited in our understanding of the mechanisms underlying its fragrance formation. Notably, terpenoids are the most prevalent volatile metabolite produced by plants. In this study, gas chromatography–mass spectrometry (GC–MS), liquid chromatography–mass spectrometry (LC–MS) and transcriptomics sequencing were conducted to analyze the synthesis mechanisms of terpenoid fragrance compounds of petals in fragrant R. fortunei Lindl. (YJ) and non-fragrant R. "Nova Zembla" (NW). The results identified that (-)-myrtenol, linalool, pinene, myrtenyl acetate, and terpineol were key floral aroma substances in YJ. Furthermore, an analysis of KEGG enrichment and differentially expressed genes (DEGs) revealed that the bud and decay stages exhibited the highest number of enriched DEGs among different aroma types, indicating these as critical stages for the synthesis of terpenoid floral compounds. In this study, a structural gene, denoted as RfFDPS, was identified as a negative regulatory gene for monoterpene accumulation and a positive regulatory gene for sesquiterpene accumulation in YJ. Utilizing subcellular localization technology, we determined that RfFDPS proteins are located in the cytoplasm. A functional analysis through transient expression and gene silencing of RfFDPS demonstrated its ability to regulate the accumulation of monoterpenes and sesquiterpenes. The overexpression of RfFDPS led to an increase in the expression of structural genes related to terpenoid synthesis, resulting in a decrease in monoterpenes and an increase in sesquiterpenes. Conversely, gene silencing had the opposite effect. In conclusion, RfFDPS plays a pivotal role in the synthesis and release of terpenoid volatile compounds in YJ petals, laying a solid theoretical foundation for the cultivation and enhancement of aromatic R. species. [ABSTRACT FROM AUTHOR]

Trametes lactinea polysaccharides have a high medicinal value; however, we still know little about the structure and bioactivity of intracellular and extracellular polysaccharides in the mycelial liquid fermentation of T. lactinea. This study analyzed the structures of intracellular (IP-1, IP-2, and IP-3) and extracellular (EP-1 and EP-2) polysaccharide components isolated from T. lactinea liquid fermentation, as well as investigated their antioxidant, antibacterial, and immunomodulatory properties. The results showed that IP-3 was the only component with a triple-helix structure, while the other four components did not possess this structure. IP3 has a higher molecular weight, flavonoid, and total phenolic content compared to other components. Both intracellular and extracellular polysaccharide components exhibited strong scavenging abilities against ABTS and DPPH radicals. The components showed limited antibacterial effects against four types of bacteria (Staphylococcus aureus, Bacillus subtilis, Erwinia carotovora, and Escherichia coli), and were found to be non-toxic to RAW264.7 cells, even promoting cell proliferation. Furthermore, within a specific concentration range, all components enhanced the phagocytic activity of RAW264.7 cells, increased the secretion of NO, TNF-α, and IL-6, and demonstrated concentration-dependent effects, with IP-3 displaying the most potent immunomodulatory activity. This study shows a high potential for the development and utilization of polysaccharides derived from the liquid fermentation of T. lactinea mycelium. [ABSTRACT FROM AUTHOR]

Building a 15-minute radius livelihood service circle from the needs of residents is a topdown process of optimizing urban layout and promoting high-quality development implemented by the government. In September 2022, Xicheng District of Beijing served as a national pilot of the 15-minute radius livelihood service circle. Based on the data of POI, urban walking network and building outline, this paper studies the coverage of commercial service facilities in the 15-minute radius livelihood service circle of Chunshu Street by using kernel density analysis and urban network analysis tools. The research shows that the commercial facilities are concentrated in Zhuangsheng Square and Dazhalan commercial district. There are large gaps in housekeeping and couriers logistics facilities, which need to be further improved. [ABSTRACT FROM AUTHOR]

Summary: Haploidentical transplantation strategies for patients with transfusion‐dependent thalassaemia (TD‐TM) remain to be investigated. In this study, 54 paediatric patients with TD‐TM were treated with a novel approach using post‐transplant cyclophosphamide (PTCy) and low‐dose methotrexate (LD‐MTX), following a myeloablative regimen. The incidence of neutrophil and platelet engraftment was 96.3% ± 2.6% and 94.4% ± 3.1% respectively. The cumulative incidence of grades II–III acute graft‐versus‐host disease (GVHD) was 13.8% ± 4.8% at 100 days. At three years, the cumulative incidence of chronic GVHD was 28.5% ± 8.5%. With a median follow‐up of 520 days (132–1325 days), the overall survival (OS) and event‐free survival (EFS) were 98.1% ± 1.8% and 90.7% ± 3.9% respectively. Compared with the low‐dose cyclophosphamide (CTX) conditioning regimen (120 mg/kg), the high‐CTX regimen (200 mg/kg) achieved a higher incidence of stable engraftment (100% vs 66.7% ± 15.7%, p = 0.003), a comparable incidence of grades II–III acute GVHD, a lower incidence of chronic GVHD (20.2% ± 8.3% vs 66.6% ± 19.2%, p = 0.011), and better overall survival (100% vs 88.9% ± 10.5%, p = 0.025) as well as EFS (95.6% ± 3.1% vs 66.7% ± 15.7%, p = 0.008). Our results using unmanipulated haploidentical grafts and PTCy with LD‐MTX in TD‐TM are encouraging. (chictr.org.cn ChiCTR1800017969) [ABSTRACT FROM AUTHOR]

Viburnumfocesides A - D, four undescribed 1- O -isovaleroylated iridoid 11- O -allosides modified with (Z / E)- p -coumaric acid, were isolated from the aqueous EtOH extract of the twigs of Viburnum foetidum var. ceanothoides , together with seven known natural products. Their structures were identified on the basis of the spectroscopic data interpretation and chemical derivation studies. Cell-based estrogen biosynthesis assays indicated that viburnumfoceside D (4), (2 S ,3 R)-2,3-dihydro-3-hydroxymethyl-7-methoxy-2-(4-hydroxy-3-methoxyphenyl)-5-benzofuranpropanol-3a- O - α -L-rhamnopyranoside (8), and (-)-eriodictyol (11) inhibit estrogen biosynthesis with IC 50 values of 5.8, 1.5, and 1.1 μM, respectively, in human ovarian granulosa-like KGN cells via decreasing the expression level of aromatase. Unlabelled Image [ABSTRACT FROM AUTHOR]

Introduction: Bougainvillea glabra " Elizabeth Angus " is a thorny woody vine or shrub. However, the hard thorns are considered a deficiency in its ornamental value. Methods: To find the genes and pathways related to the hardening process of the thorns on the stems of B. glabra , the eukaryotic unreferenced transcriptome sequencing analysis was conducted to explore the 3 stages of the thorn-hardening process. Total RNA was extracted from thorns and stems, and transcriptome libraries were constructed and sequenced using unreferenced Illumina sequencing. Results: Gene function annotation was performed using various databases, resulting in 8937 co-annotated genes. The density distribution of Fragments Per Kilobase of transcript per Million mapped reads (FPKM) depicted the overall gene expression patterns. The study found that stage 2 as the period of highest gene expression activity during the thorns hardening process in B. glabra . Differential expression analysis revealed that during thorn-hardening, 1045 genes up-regulated and 391 genes down-regulated significantly in thorns at stage 2 compared to stage 1 (early stage of thorns formation). Meanwhile, 938 genes up-regulated and 784 genes down-regulated significantly in stems. At stage 3, as thorns became harder, 63 genes exhibited notable expression increase and 98 genes' expression decreased obviously within thorns, and 46 genes up-regulated and 29 genes down-regulated in stems, compared to stage 2. Phenylpropanoid biosynthesis was the key step in the hardening process of the thorns of B. glabra . The formation and hardening of thorns on the stem of B. glabra was a process in which lignin gradually accumulated in the thorns, and several genes were involved in this process. They include PAL (EC:4.3.1.24), CYP73A (EC:1.14.14.91), 4CL (EC:6.2.1.12), CCR (EC:1.2.1.44), CAD (EC:1.1.1.195) and POX (EC:1.11.1.7). Discussion: This transcriptome analysis offers insights into the molecular mechanisms underlying thorns development in this plant species., Competing Interests: Author QC was employed by Nanning GoldTech Biotechnology Ltd. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Sun, Wang, Li, Gong, Yang, Yang, Chen, Li, Lu, Chen and Lin.)

The aim of this paper is to study the methods of 3D visualization and the 3D interactive clipping of CT/MRI image sequence in arbitrary orientation based on the Visualization Toolkit (VTK). A new method for 3D CT/MRI reconstructed image clipping is presented, which can clip 3D object and 3D space of medical image sequence to observe the inner structure using 3D widget for manipulating an infinite plane. Experiment results show that the proposed method can implement 3D interactive clipping of medical image effectively and get satisfied results with good quality in short time.