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12. Identification and analysis of main viral species and mixed infection types of tobacco in Anshun tobacco growing area of Guizhou Province.

Author

PAN Shouhui, XUE Yuan, ZHANG Hai, HE Haiyong, GONG Shuangjun, YANG Fuhan, ZHAO Dailin, LI Jiye, and TAN Qingqun

Abstract

To clarify the main species and infection types of tobacco viral diseases in Anshun tobacco growing areas of Guizhou Province, 306 diseased samples with symptoms such as mosaic, necrotic veins, abnormal shapes, lightning spots, and yellow spots were collected from Anshun tobacco growing areas in 2023. RT-PCR was performed using specific primers for 5 major viral diseases of tobacco mosaic virus (TMV), cucumber mosaic virus (CMV), potato Y virus (PVY), tobacco vein banding mosaic virus (TVBMV) and chilli veinal mottle virus (ChiVMV), and the detection rates of the viruses were calculated and the mixed infection types were analyzed. The results showed that the total detection rate of the viral diseases was 89.87%, and among all the diseases, TMV (81.70%)ranked the highest, followed by ChiVMV (67.97%), and CMV ranked the lowest (16.34%). The main type of infection was mixed infection with a detection rate of 68.30% . Among them, ChiVMV + TMV was the main type with a detection rate of 22.88%, followed by ChiVMV+TMV+TVBMV, ChiVMV+TMV+PVY, and ChiVMV+ TMV+CMV with detection rates of 11.11%, 10.46%, and 9.15%, respectively. TMV and ChiVMV were the dominant viral species of the tobacco viral diseases from the area, mixed infection occurred frequently and ChiVMV+TMV was the main mixed type. [ABSTRACT FROM AUTHOR]

Published
2024
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19. A combination of annual and nonannual forces drive respiratory disease in the tropics

Author

Yang, Fuhan, primary, Servadio, Joseph L, additional, Thanh, Nguyen Thi Le, additional, Lam, Ha Minh, additional, Choisy, Marc, additional, Thai, Pham Quang, additional, Thao, Tran Thi Nhu, additional, Vy, Nguyen Ha Thao, additional, Phuong, Huynh Thi, additional, Nguyen, Tran Dang, additional, Tam, Dong Thi Hoai, additional, Hanks, Ephraim M, additional, Vinh, Ha, additional, Bjornstad, Ottar N, additional, Chau, Nguyen Van Vinh, additional, and Boni, Maciej F, additional

Published
2023
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22. The value of contrast-enhanced ultrasound and magnetic resonance imaging in the diagnosis of bladder cancer

Author

Li, Cheng, Gu, Zhuoran, Ni, Peiqian, Zhang, Wentao, Yang, Fuhan, Li, Wei, Yao, Xudong, and Chen, Yifan

Subjects
Cancer -- Diagnosis, Bladder cancer -- Diagnosis, Magnetic resonance imaging -- Usage, Diagnosis, Ultrasonic -- Usage, Health
Abstract

Byline: Cheng. Li, Zhuoran. Gu, Peiqian. Ni, Wentao. Zhang, Fuhan. Yang, Wei. Li, Xudong. Yao, Yifan. Chen Objective: Imaging examination, tumor marker detection, bladder biopsy, and other methods are the [...]

Published
2021

28. SARS-CoV-2 Attack Rate and Population Immunity in Southern New England, March 2020 to May 2021

Author

Tran, Thu Nguyen-Anh, primary, Wikle, Nathan B., additional, Yang, Fuhan, additional, Inam, Haider, additional, Leighow, Scott, additional, Gentilesco, Bethany, additional, Chan, Philip, additional, Albert, Emmy, additional, Strong, Emily R., additional, Pritchard, Justin R., additional, Hanage, William P., additional, Hanks, Ephraim M., additional, Crawford, Forrest W., additional, and Boni, Maciej F., additional

Published
2022
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30. Recent Trends in the Incidence of Clear Cell Adenocarcinoma and Survival Outcomes: A SEER Analysis

Author

Guo, Yadong, primary, Shrestha, Anil, additional, Maskey, Niraj, additional, Dong, Xiaohui, additional, Zheng, Zongtai, additional, Yang, Fuhan, additional, Wang, Ruiliang, additional, Ma, Wenchao, additional, Liu, Ji, additional, Li, Cheng, additional, Zhang, Wentao, additional, Mao, Shiyu, additional, Zhang, Aihong, additional, Liu, Shenghua, additional, and Yao, Xudong, additional

Published
2022
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31. sj-docx-1-tam-10.1177_17588359221108690 – Supplemental material for Gender dimorphism in survival of patients with lymph node metastasis of bladder cancer

Author

Guo, Yadong, Zheng, Zongtai, Zhang, Wentao, Mao, Shiyu, Yang, Fuhan, Li, Wei, Yan, Yang, and Yao, Xudong

Subjects
110203 Respiratory Diseases, FOS: Clinical medicine, 111702 Aged Health Care, FOS: Health sciences, human activities, humanities, 111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified, 111299 Oncology and Carcinogenesis not elsewhere classified
Abstract

Supplemental material, sj-docx-1-tam-10.1177_17588359221108690 for Gender dimorphism in survival of patients with lymph node metastasis of bladder cancer by Yadong Guo, Zongtai Zheng, Wentao Zhang, Shiyu Mao, Fuhan Yang, Wei Li, Yang Yan and Xudong Yao in Therapeutic Advances in Medical Oncology

Published
2022
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33. SARS-CoV-2 epidemic after social and economic reopening in three U.S. states reveals shifts in age structure and clinical characteristics

Author

Wikle, Nathan B., primary, Tran, Thu Nguyen-Anh, additional, Gentilesco, Bethany, additional, Leighow, Scott M., additional, Albert, Emmy, additional, Strong, Emily R., additional, Brinda, Karel, additional, Inam, Haider, additional, Yang, Fuhan, additional, Hossain, Sajid, additional, Chan, Philip, additional, Hanage, William P., additional, Messick, Maria, additional, Pritchard, Justin R., additional, Hanks, Ephraim M., additional, and Boni, Maciej F., additional

Published
2022
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37. Inhibition of TGF-β repairs spinal cord injury by attenuating EphrinB2 expressing through inducing miR-484 from fibroblast

Author

Pan, Dayu, Yang, Fuhan, Zhu, Shibo, Li, Yongjin, Ning, Guangzhi, and Feng, Shiqing

Subjects
QH573-671, Neoplasms. Tumors. Oncology. Including cancer and carcinogens, Diseases of the nervous system, Spinal cord injury, Cytology, RC254-282, Article
Abstract

Spinal cord injury (SCI) can lead to severe loss of motor and sensory function with high disability and mortality. The effective treatment of SCI remains unknown. Here we find systemic injection of TGF-β neutralizing antibody induces the protection of axon growth, survival of neurons, and functional recovery, whereas erythropoietin-producing hepatoma interactor B2 (EphrinB2) expression and fibroblasts distribution are attenuated. Knockout of TGF-β type II receptor in fibroblasts can also decrease EphrinB2 expression and improve spinal cord injury recovery. Moreover, miR-488 was confirmed to be the most upregulated gene related to EphrinB2 releasing in fibroblasts after SCI and miR-488 initiates EphrinB2 expression and physical barrier building through MAPK signaling after SCI. Our study points toward elevated levels of active TGF-β as inducer and promoters of fibroblasts distribution, fibrotic scar formation, and EphrinB2 expression, and deletion of global TGF-β or the receptor of TGF-β in Col1α2 lineage fibroblasts significantly improve functional recovery after SCI, which suggest that TGF-β might be a therapeutic target in SCI.

Published
2021

38. SARS-CoV-2 attack rate and population immunity in southern New England, March 2020 - May 2021

Author

Tran, Thu Nguyen-Anh, primary, Wikle, Nathan B, additional, Yang, Fuhan, additional, Inam, Haider, additional, Leighow, Scott M, additional, Gentilesco, Bethany, additional, Chan, Philip, additional, Albert, Emmy, additional, Strong, Emily R, additional, Pritchard, Justin R, additional, Hanage, William P, additional, Hanks, Ephraim M, additional, Crawford, Forrest W., additional, and Boni, Maciej F, additional

Published
2021
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42. Sulfatase 2 Affects Polarization of M2 Macrophages through the IL-8/JAK2/STAT3 Pathway in Bladder Cancer.

Author

Zhang, Wentao, Yang, Fuhan, Zheng, Zongtai, Li, Cheng, Mao, Shiyu, Wu, Yuan, Wang, Ruiliang, Zhang, Junfeng, Zhang, Yue, Wang, Hong, Li, Wei, Huang, Jianhua, and Yao, Xudong

Subjects
*INTERLEUKINS, *STAT proteins, *DISEASE progression, *IN vitro studies, *BIOLOGICAL models, *FLOW cytometry, *REVERSE transcriptase polymerase chain reaction, *IN vivo studies, *SEQUENCE analysis, *ANIMAL experimentation, *WESTERN immunoblotting, *COLONY-forming units assay, *IMMUNOHISTOCHEMISTRY, *LOG-rank test, *ONE-way analysis of variance, *MACROPHAGES, *MANN Whitney U Test, *CELLULAR signal transduction, *JANUS kinases, *CANCER patients, *CELL proliferation, *ENZYME-linked immunosorbent assay, *DESCRIPTIVE statistics, *SURVIVAL analysis (Biometry), *KAPLAN-Meier estimator, *RESEARCH funding, *TUMOR markers, *NEUROTRANSMITTER uptake inhibitors, *CELL lines, *DATA analysis software, *MICE, *OVERALL survival, BLADDER tumors
Abstract

Simple Summary: At present, the immunotherapy and immune microenvironment of bladder cancer have attracted more and more attention from clinicians and researchers. In this study, the high expression of SULF2 by RNA sequencing of tissues from 90 bladder cancer patients was associated with poor prognosis in patients. We further found that a high expression of SULF2 can promote the polarization of macrophages to M2. In mechanistic studies, we found that SULF2 can promote the secretion of IL-8 through Wnt/β-catenin. IL-8 promotes the transcription of CD163 and CD206 in the microenvironment, ultimately leading to the polarization of macrophages to M2 macrophages. This study elucidates the effect of SULF2 on the polarization of macrophages in the tumor microenvironment. Sulfatase 2 (SULF2) affects the occurrence and development of cancer by regulating HSPG-binding factors. However, the mechanism of SULF2 in bladder cancer (BCa) is unknown. To determine this, we analyzed the RNA sequencing of 90 patients with BCa. The results showed that the expression of SULF2 was closely related to the prognosis of BCa. Moreover, in vivo and in vitro experiments revealed that SULF2 promotes tumor proliferation and invasion. Furthermore, using a mouse orthotopic BCa model and flow cytometric analysis, we identified that SULF2 affects the polarization of macrophages. Mechanism studies clarified that SULF2 promoted the release of HSPG-binding factors, such as IL-8, in the microenvironment through β-catenin. Meanwhile, IL-8 activated the JAK2/STAT3 pathway of macrophages to promote the expression of CD163 and CD206, thereby regulating the polarization of macrophages to the M2-type. Conclusively, these results indicate that SULF2 plays an important role in regulating the microenvironment of BCa and promotes the polarization of macrophages to the M2-type by secreting IL-8, which further deepens the malignant progression of BCa. [ABSTRACT FROM AUTHOR]

Published
2023
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47. Additional file 1 of Hsa_circ_0004296 inhibits metastasis of prostate cancer by interacting with EIF4A3 to prevent nuclear export of ETS1 mRNA

Author

Mao, Shiyu, Zhang, Wentao, Yang, Fuhan, Guo, Yadong, Wang, Hong, Wu, Yuan, Wang, Ruiliang, Maskey, Niraj, Zheng, Zongtai, Li, Cheng, Ma, Wenchao, Zhang, Junfeng, Yan, Yang, and Yao, Xudong

Subjects
genetic processes, information science, natural sciences, eye diseases
Abstract

Additional file 1: File S1. The Sanger sequencing of the linear circ_0004296 RNA sequence carried on the plasmid.

Published
2021
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48. Additional file 6 of Hsa_circ_0004296 inhibits metastasis of prostate cancer by interacting with EIF4A3 to prevent nuclear export of ETS1 mRNA

Author

Mao, Shiyu, Zhang, Wentao, Yang, Fuhan, Guo, Yadong, Wang, Hong, Wu, Yuan, Wang, Ruiliang, Maskey, Niraj, Zheng, Zongtai, Li, Cheng, Ma, Wenchao, Zhang, Junfeng, Yan, Yang, and Yao, Xudong

Subjects
urologic and male genital diseases
Abstract

Additional file 6: Figure S3. A. Knockdown of EIF4A3 had no effect on circ_0004296 expression. B. The efficiency of ETS1 knockdown at protein level. C. The efficiency of ETS1 overexpression at protein level. D. Colony formation assays were used to measure viability of PCa cells after ETS1 knockdown. E. The migration abilities of PCa cells were analyzed by Transwell assays for migration after ETS1 knockdown. Scale bars =100um. F. Colony formation assays were used to measure viability of PCa cells after ETS1 overexpression. G. The migration abilities of PCa cells were analyzed by Transwell assays for migration after ETS1 overexpression. Scale bars =100um. H. Kaplan-Meier analysis for different ETS1 protein expression in PCa patients. I, J. Luciferase-labeled stably DU145-overexpressing circ_0004296 and luciferase-labeled DU145-vector cells were constructed. Scale bars =200um. *P

Published
2021
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49. Additional file 5 of Hsa_circ_0004296 inhibits metastasis of prostate cancer by interacting with EIF4A3 to prevent nuclear export of ETS1 mRNA

Author

Mao, Shiyu, Zhang, Wentao, Yang, Fuhan, Guo, Yadong, Wang, Hong, Wu, Yuan, Wang, Ruiliang, Maskey, Niraj, Zheng, Zongtai, Li, Cheng, Ma, Wenchao, Zhang, Junfeng, Yan, Yang, and Yao, Xudong

Subjects
urologic and male genital diseases
Abstract

Additional file 5: Figure S2. The circ_0004296 knockdown promoted the proliferation, migration, invasion, and EMT induction of PCa cells. A. Schematic illustration of designed siRNAs (siRNA#1, siRNA#2, and siRNA#3) targeting the back-splice junction of circ_0004296. B. The expression of circ_0004296 and ETS1 was detected by qRT-PCR in PC3 and DU145 cells transfected with siRNA-circ_0004296 or si-NC. C, D. CCK8 and colony formation assays were performed to measure the viability of PCa cells after circ_0004296 knockdown. E-H. The migration and invasion abilities of PCa cells were analyzed by wound healing and Transwell assays for migration and invasion after circ_0004296 knockdown. Scale bars =100um. I. Western blotting assay was performed to detected the expression of EMT-related proteins, namely E-cadherin, N-cadherin, Vimentin, and Snail, after circ_0004296 knockdown. J. Protein profiling was used to screen specific proteins bound by circ_0004296 in PCa cells. *P

Published
2021
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