205 results on '"Yan-Ling Chen"'
Search Results
2. Latent profile analysis and influencing factors of quality of life in pregnant women with gestational diabetes mellitus
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Xin-yi Zhou, Yan-feng Wang, Jie-mei Yang, Li-yuan Yang, Wei-jia Zhao, Yan-ling Chen, and Qiao-hong Yang
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Gestational Diabetes Mellitus ,Pregnant woman ,Quality of life ,Latent profile analysis ,Gynecology and obstetrics ,RG1-991 - Abstract
Abstract Purpose Gestational diabetes mellitus (GDM) negatively affects the quality of life of pregnant women and is influenced by several factors. Research to date treats pregnant women with gestational diabetes as a homogeneous group based on their quality of life. We attempted to identify subgroups based on self-reported quality of life and explored variables associated with subgroups. Methods From September 1, 2020 to November 29, 2020, pregnant women with GDM from two hospitals in Guangdong Province were selected as subjects by convenience sampling method. Medical records provided sociodemographic data, duration of GDM, pregnancy status, and family history of diabetes. Participants completed validated questionnaires for quality of life, anxiety and depression. Latent profile analysis was used to identify profiles of quality of life in pregnant women with GDM, and then a mixed regression method was used to analyze the influencing factors of different profiles. Results A total of 279 valid questionnaires were collected. The results of the latent profile analysis showed that the quality of life of pregnant women with GDM could be divided into two profiles: C1 “high worry-high support” group (75.6%) and C2 “low worry-low support” group (24.4%). Daily exercise duration and depression degree are negative influencing factors, making it easier to enter the C1 group (p
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- 2023
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3. IL-8 is a novel prometastatic chemokine in intrahepatic cholangiocarcinoma that induces CXCR2-PI3K/AKT signaling upon CD97 activation
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Ze-Wu Meng, Lei Zhang, Xin-Ran Cai, Xing Wang, Fei-Fei She, and Yan-Ling Chen
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Medicine ,Science - Abstract
Abstract Intrahepatic cholangiocarcinoma (ICC) is a rare but highly aggressive malignant tumor arising within the liver, with a 5-year survival rate of only 20–40% after surgery. The role of interleukin-8 (IL-8) in ICC progression remains elusive. A transcriptomic approach based on IL-8 stimulation first revealed significant upregulation of the prometastatic gene CD97 and key epithelial–mesenchymal transition (EMT) factors E-cadherin and vimentin. Immunohistochemistry of 125 ICC tissues confirmed the positive correlation between IL-8 and CD97. Multivariable Cox regression indicated that they are both independent predictors of ICC prognosis. Mechanistically, IL-8 treatment induced CD97 expression at 50 and 100 ng/ml in QBC-939 and QBE cells, respectively. Moreover, the induction of cell migration and invasion upon IL-8 treatment was attenuated by CD97 RNA interference, and the expression of EMT-associated genes was dramatically inhibited. To determine whether CXCR1 or CXCR2 are downstream effectors of IL-8, siCXCR2 was applied and shown to significantly attenuate the oncogenic effects of IL-8 by inhibiting the phosphorylation of PI3K/AKT. Finally, the induction of CD97 expression by the PI3K pathway was verified by treatment with the inhibitor LY294002. In vivo, the significant tumor growth and lung metastasis effects induced by intraperitoneal injection of IL-8 were greatly inhibited by silencing CD97 in nude mice. Collectively, the study presents a novel mechanism of the IL-8-CXCR2-PI3K/AKT axis in regulating CD97 expression, which leads to ICC metastasis mainly through EMT. The study may provide alternatives for targeting the tumor microenvironment in metastatic ICC.
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- 2023
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4. Non-alcoholic fatty liver disease causally affects the brain cortical structure: a Mendelian randomization study
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Yu-Kai Lin, Xin-Ran Cai, Jiang-Zhi Chen, Hai-Jie Hong, Kai Tu, Yan-Ling Chen, and Qiang Du
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Mendelian randomization ,non-alcoholic fatty liver disease ,brain cortical structure ,liver–brain axis ,genome-wide association study ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
BackgroundReduced brain volume, impaired cognition, and possibly a range of psychoneurological disorders have been reported in patients with non-alcoholic fatty liver disease (NAFLD); however, no underlying cause has been specified. Here, Mendelian randomization (MR) was employed to determine the causative NAFLD effects on cortical structure.MethodsWe used pooled-level data from FinnGen’s published genome-wide association study (GWAS) of NAFLD (1908 cases and 340,591 healthy controls), as well as published GWAS with NAFLD activity score (NAS) and fibrosis stage-associated SNPs as genetic tools, in addition to the Enigma Consortium data from 51,665 patients, were used to assess genetic susceptibility in relation to changes with cortical thickness (TH) and surface area (SA). A main estimate was made by means of inverse variance weighted (IVW), while heterogeneity and pleiotropy were detected using MR-Egger, weighted median, and MR Pleiotropy RESidual Sum and Outlier to perform a two-sample MR analysis.ResultsAt the global level, NAFLD reduced SA (beta = −586.72 mm2, se = 217.73, p = 0.007) and several changes in the cortical structure of the cerebral gyrus were found, with no detectable pleiotropy.ConclusionNAFLD causally affects cortical structures, which supports the presence of an intricate liver–brain axis.
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- 2024
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5. Short term outcomes of extremely low birth weight infants from a multicenter cohort study in Guangdong of China
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Chun-Hong Jia, Zhou-Shan Feng, Xiao-Jun Lin, Qi-Liang Cui, Sha-Sha Han, Ya Jin, Guo-Sheng Liu, Chuan-Zhong Yang, Xiao-Tong Ye, Yi-Heng Dai, Wei-Yi Liang, Xiu-Zhen Ye, Jing Mo, Lu Ding, Ben-Qing Wu, Hong-Xiang Chen, Chi-Wang Li, Zhe Zhang, Xiao Rong, Wei-Min Huang, Wei Shen, Bing-Yan Yang, Jun-Feng Lv, Le-Ying Huo, Hui-Wen Huang, Hong-Ping Rao, Wen-Kang Yan, Yong Yang, Xue-Jun Ren, Dong Liu, Fang-Fang Wang, Shi-Guang Diao, Xiao-Yan Liu, Chu-Ming You, Qiong Meng, Bin Wang, Li-Juan Zhang, Yu-Ge Huang, Dang Ao, Wei-Zhong Li, Jie-Ling Chen, Yan-Ling Chen, Wei Li, Zhi-Feng Chen, Yue-Qin Ding, Xiao-Yu Li, Yue-Fang Huang, Ni-Yang Lin, Yang-Fan Cai, Zhong-He Wan, Yi Ban, Bo Bai, Guang-Hong Li, Yue-Xiu Yan, and Fan Wu
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Medicine ,Science - Abstract
Abstract With the increase in extremely low birth weight (ELBW) infants, their outcome attracted worldwide attention. However, in China, the related studies are rare. The hospitalized records of ELBW infants discharged from twenty-six neonatal intensive care units in Guangdong Province of China during 2008–2017 were analyzed. A total of 2575 ELBW infants were enrolled and the overall survival rate was 55.11%. From 2008 to 2017, the number of ELBW infants increased rapidly from 91 to 466, and the survival rate improved steadily from 41.76% to 62.02%. Increased survival is closely related to birth weight (BW), regional economic development, and specialized hospital. The incidence of complications was neonatal respiratory distress syndrome (85.2%), oxygen dependency at 28 days (63.7%), retinopathy of prematurity (39.3%), intraventricular hemorrhage (29.4%), necrotizing enterocolitis (12.0%), and periventricular leukomalacia (8.0%). Among the 1156 nonsurvivors, 90.0% of infants died during the neonatal period (≤ 28 days). A total of 768 ELBW infants died after treatment withdrawal, for reasons of economic and/or poor outcome. The number of ELBW infants is increasing in Guangdong Province of China, and the overall survival rate is improving steadily.
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- 2022
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6. Super-enhancer profiling identifies novel critical and targetable cancer survival gene LYL1 in pediatric acute myeloid leukemia
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Fang Fang, Jun Lu, Xu Sang, Yan-Fang Tao, Jian-Wei Wang, Zi-Mu Zhang, Yong-Ping Zhang, Xiao-Lu Li, Yi Xie, Shui-Yan Wu, Xin-Ran Chu, Gen Li, Di Wu, Yan-Ling Chen, Juan-Juan Yu, Si-qi Jia, Chen-xi Feng, Yuan-Yuan Tian, Zhi-Heng Li, Jing Ling, Shao-Yan Hu, and Jian Pan
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Acute myeloid leukemia ,Super-enhancer ,LYL1 ,GNE-987 ,ChIP-Seq analysis ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Acute myeloid leukemia (AML) is a myeloid neoplasm makes up 7.6% of hematopoietic malignancies. Super-enhancers (SEs) represent a special group of enhancers, which have been reported in multiple cell types. In this study, we explored super-enhancer profiling through ChIP-Seq analysis of AML samples and AML cell lines, followed by functional analysis. Methods ChIP-seq analysis for H3K27ac was performed in 11 AML samples, 7 T-ALL samples, 8 B-ALL samples, and in NB4 cell line. Genes and pathways affected by GNE-987 treatment were identified by gene expression analysis using RNA-seq. One of the genes associated with super-enhancer and affected by GNE-987 treatment was LYL1 basic helix-loop-helix family member (LYL1). shRNA mediated gene interference was used to down-regulate the expression of LYL1 in AML cell lines, and knockdown efficiency was detected by RT-qPCR and western blotting. The effect of knockdown on the growth of AML cell lines was evaluated by CCK-8. Western blotting was used to detect PARP cleavage, and flow cytometry were used to determine the effect of knockdown on apoptosis of AML cells. Results We identified a total of 200 genes which were commonly associated with super-enhancers in ≧10 AML samples, and were found enriched in regulation of transcription. Using the BRD4 inhibitor GNE-987, we assessed the dependence of AML cells on transcriptional activation for growth and found GNE-987 treatment predominantly inhibits cell growth in AML cells. Moreover, 20 candidate genes were selected by super-enhancer profile and gene expression profile and among which LYL1 was observed to promote cell growth and survival in human AML cells. Conclusions In summary, we identified 200 common super-enhancer-associated genes in AML samples, and a series of those genes are cancer genes. We also found GNE-987 treatment downregulates the expression of super-enhancer-associated genes in AML cells, including the expression of LYL1. Further functional analysis indicated that LYL1 is required for AML cell growth and survival. These findings promote understanding of AML pathophysiology and elucidated an important role of LYL1 in AML progression.
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- 2022
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7. Infestation and distribution of chigger mites on Chevrieri's field mouse (Apodemus chevrieri) in Southwest China
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Yan-Ling Chen, Xian-Guo Guo, Tian-Guang Ren, Lei Zhang, Rong Fan, Cheng-Fu Zhao, Zhi-Wei Zhang, Ke-Yu Mao, Xiao-Bin Huang, and Ti-Jun Qian
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Acari ,Chigger mite ,Ectoparasite ,Rodent ,Apodemus chevrieri ,Southwest China ,Zoology ,QL1-991 - Abstract
Based on a long-term field investigation on chigger mites in southwest China from 2001 to 2019, the present study analyzed the infestation and distribution of chigger mites on the Chevrieri's field mouse (Apodemus chevrieri) in the region. A total of 12,516 individuals of chigger mites were collected from 1981 A. chevrieri mice, and 12,281 chiggers were identified as 107 species, 11 genera and 3 subfamilies in 2 families, which revealed a high species diversity of the mites on A. chevrieri mice. Of 1981 A. chevrieri mice, 633 ones were infested with chiggers with a relatively high overall prevalence (PM = 31.95%), mean abundance (MA = 6.32) and mean intensity (MI = 19.77). Of the 107 chigger species identified from A. chevrieri mice, three ones were the most dominant and they were Leptrombidium scutellare, L. densipunctatum and L. cricethrionis, which showed aggregated distribution among different individuals of the mice. A slightly positive association existed between every two dominant chigger species, which implied that the dominant chigger species tend to co-exist on A. chevrieri. The infestations of A. chevrieri with chiggers varied in different latitudes, altitudes and landscapes and they showed some heterogeneity along different environmental gradients. The logistic regression analysis showed that the risk factors for chigger infestations on A. chevrieri were landscapes, ages and altitudes, which implied that the environmental factors and host ages could influence the infestations of the mice with the mites. A theoretical curve of the species abundance distribution of chigger mites on A. chevrieri was successfully fitted by Preston's lognormal model, suggesting that the species abundance distribution conforms to the lognormal distribution pattern. The expected total species of chigger mites on A. chevrieri was roughly estimated to be 136 species and about 29 rare chigger species were probably missed in the sampling field investigation.
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- 2022
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8. TIMP3 induces gene expression partly through PI3K and their association with vascularization and heart rate
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Zi-Meng Xia, Meng-Yu Song, Yan-Ling Chen, Guozhen Cui, and Dong Fan
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tissue inhibitor of metalloproteinase 3 ,angiogenesis ,heart rate ,cardiovascular system ,signal pathway ,gene expression ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
BackgroundTissue inhibitor of metalloproteinase 3 (TIMP3) was recently demonstrated capable to regulate some gene expression in a myocardial infarction model. Here we aim to explore the gene expression profile in TIMP3-treated cardiomyocytes and related potential cardiovascular functions.MethodsTotal RNA extracted from cultured neonatal rat ventricular myocytes (NRVMs) were used for RNA sequencing analysis and real-time PCR. KEGG pathway enrichment assay and Ingenuity Pathway Analysis (IPA) were performed to study the signaling pathways and downstream effects. Western blot was used to detect phosphorylation of protein kinase B (Akt). A Cell Counting Kit-8 assay was employed to evaluate the proliferation of human umbilical vein endothelial cells (HUVECs). Contraction rate of NRVMs was measured with microscopy.ResultsRNA sequencing data showed that expression of 2,526 genes were significantly modulated by recombinant TIMP3 (rTIMP3, 100 ng/ml) in NRVMs. Some differentially expressed genes (DEGs) were validated with real-time PCR. Several KEGG pathways including the phosphoinositide-3-kinase (PI3K)-Akt pathway were significantly regulated by rTIMP3. Phosphorylation of Akt was increased by rTIMP3 and a PI3K inhibitor LY294002 suppressed rTIMP3-induced up-regulation of some genes. Some DEGs were predicted by IPA to increase vascularization, and some to decrease heart rate. RTIMP3 could reduce the contraction rate of NRVMs and its conditioned media increased the proliferation of HUVECs.ConclusionTIMP3 can regulate expression of multiple genes partly through PI3K. Some DEGs were associated with activation of vascularization and some with heart rate reduction. This study suggests that TIMP3 can potentially modulate cardiovascular functions via DEGs.
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- 2023
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9. BRD4 inhibitor GNE987 exerts anti-cancer effects by targeting super-enhancers in neuroblastoma
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Yan-Ling Chen, Xiao-Lu Li, Gen Li, Yan-Fang Tao, Ran Zhuo, Hai-Bo Cao, Wan-yan Jiao, Zhi-Heng Li, Zhen-Hong Zhu, Fang Fang, Yi Xie, Xin-Mei Liao, Di Wu, Hai-Rong Wang, Juan-Juan Yu, Si-Qi Jia, Yang Yang, Chen-Xi Feng, Peng-Cheng Yang, Xiao-Dong Fei, Jian-Wei Wang, Yun-Yun Xu, Guang-Hui Qian, Zi-Mu Zhang, and Jian Pan
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BRD4 ,Neuroblastoma ,Broad H3K27ac domain ,Super-enhancer ,PROTAC ,Biotechnology ,TP248.13-248.65 ,Biology (General) ,QH301-705.5 ,Biochemistry ,QD415-436 - Abstract
Abstract Background Neuroblastoma (NB) is a common extracranial malignancy with high mortality in children. Recently, super-enhancers (SEs) have been reported to play a critical role in the tumorigenesis and development of NB via regulating a wide range of oncogenes Thus, the synthesis and identification of chemical inhibitors specifically targeting SEs are of great urgency for the clinical therapy of NB. This study aimed to characterize the activity of the SEs inhibitor GNE987, which targets BRD4, in NB. Results In this study, we found that nanomolar concentrations of GNE987 markedly diminished NB cell proliferation and survival via degrading BRD4. Meanwhile, GNE987 significantly induced NB cell apoptosis and cell cycle arrest. Consistent with in vitro results, GNE987 administration (0.25 mg/kg) markedly decreased the tumor size in the xenograft model, with less toxicity, and induced similar BRD4 protein degradation to that observed in vitro. Mechanically, GNE987 led to significant downregulation of hallmark genes associated with MYC and the global disruption of the SEs landscape in NB cells. Moreover, a novel candidate oncogenic transcript, FAM163A, was identified through analysis of the RNA-seq and ChIP-seq data. FAM163A is abnormally transcribed by SEs, playing an important role in NB occurrence and development. Conclusion GNE987 destroyed the abnormal transcriptional regulation of oncogenes in NB by downregulating BRD4, which could be a potential therapeutic candidate for NB.
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- 2022
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10. SAPCD2 promotes neuroblastoma progression by altering the subcellular distribution of E2F7
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Zi-Mu Zhang, Hai-Bo Cao, Zhi-Heng Li, Ran Zhuo, Yan-Fang Tao, Xiao-Lu Li, Gen Li, Xin-Mei Liao, Fang Fang, Yi Xie, Di Wu, Hai-Rong Wang, Jian-Wei Wang, Yan-Ling Chen, Juan-Juan Yu, Si-Qi Jia, Ran-Dong Yang, Xin-Yi Guo, Yang Yang, Chen-Xi Feng, Yun-Yun Xu, Guang-Hui Qian, and Jian Pan
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Cytology ,QH573-671 - Abstract
Abstract Recent studies uncovered the emerging roles of SAPCD2 (suppressor anaphase-promoting complex domain containing 2) in several types of human cancer. However, the functions and underlying mechanisms of SAPCD2 in the progression of neuroblastoma (NB) remain elusive. Herein, through integrative analysis of public datasets and regulatory network of GSK-J4, a small-molecule drug with anti-NB activity, we identified SAPCD2 as an appealing target with a high connection to poor prognosis in NB. SAPCD2 promoted NB progression in vitro and in vivo. Mechanistically, SAPCD2 could directly bind to cytoplasmic E2F7 but not E2F1, alter the subcellular distribution of E2F7 and regulate E2F activity. Among the E2F family members, the roles of E2F7 in NB are poorly understood. We found that an increasing level of nuclear E2F7 was induced by SAPCD2 knockdown, thereby affecting the expression of genes involved in the cell cycle and chromosome instability. In addition, Selinexor (KTP-330), a clinically available inhibitor of exportin 1 (XPO1), could induce nuclear accumulation of E2F7 and suppress the growth of NB. Overall, our studies suggested a previously unrecognized role of SAPCD2 in the E2F signaling pathway and a potential therapeutic approach for NB, as well as clues for understanding the differences in subcellular distribution of E2F1 and E2F7 during their nucleocytoplasmic shuttling.
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- 2022
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11. CEBPG promotes acute myeloid leukemia progression by enhancing EIF4EBP1
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You Jiang, Shui-Yan Wu, Yan-Ling Chen, Zi-Mu Zhang, Yan-Fang Tao, Yi Xie, Xin-Mei Liao, Xiao-Lu Li, Gen Li, Di Wu, Hai-Rong Wang, Ran Zuo, Hai-Bo Cao, Jing-Jing Pan, Juan-Juan Yu, Si-Qi Jia, Zheng Zhang, Xin-Ran Chu, Yong-Ping Zhang, Chen-xi Feng, Jian-Wei Wang, Shao-Yan Hu, Zhi-Heng Li, Jian Pan, Fang Fang, and Jun Lu
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CEBPG ,EIF4EBP1 ,Acute myeloid leukemia ,Proliferation ,Apoptosis ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 ,Cytology ,QH573-671 - Abstract
Abstract Background Acute myeloid leukemia (AML) is a myeloid neoplasm accounts for 7.6% of hematopoietic malignancies. AML is a complex disease, and understanding its pathophysiology is contributing to the improvement in the treatment and prognosis of AML. In this study, we assessed the expression profile and molecular functions of CCAAT enhancer binding protein gamma (CEBPG), a gene implicated in myeloid differentiation and AML progression. Methods shRNA mediated gene interference was used to down-regulate the expression of CEBPG in AML cell lines, and knockdown efficiency was detected by RT-qPCR and western blotting. The effect of knockdown on the growth of AML cell lines was evaluated by CCK-8. Western blotting was used to detect PARP cleavage, and flow cytometry were used to determine the effect of knockdown on apoptosis of AML cells. Genes and pathways affected by knockdown of CEBPG were identified by gene expression analysis using RNA-seq. One of the genes affected by knockdown of CEBPG was Eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1), a known repressor of translation. Knockdown of EIF4EBP1 was used to assess its potential role in AML progression downstream of CEBPG. Results We explored the ChIP-Seq data of AML cell lines and non-AML hematopoietic cells, and found CEBPG was activated through its distal enhancer in AML cell lines. Using the public transcriptomic dataset, the Cancer Cell Line Encyclopedia (CCLE) and western blotting, we also found CEBPG was overexpressed in AML. Moreover, we observed that CEBPG promotes AML cell proliferation by activating EIF4EBP1, thus contributing to the progression of AML. These findings indicate that CEBPG could act as a potential therapeutic target for AML patients. Conclusion In summary, we systematically explored the molecular characteristics of CEBPG in AML and identified CEBPG as a potential therapeutic target for AML patients. Our findings provide novel insights into the pathophysiology of AML and indicate a key role for CEBPG in promoting AML progression.
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- 2021
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12. The clinical significance of preoperative serum fibrinogen levels and platelet counts in patients with gallbladder carcinoma
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Peng Cao, Lei Jiang, Liang-Yi Zhou, and Yan-Ling Chen
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Gallbladder carcinoma ,Hyperfibrinogenemia ,Thrombocytosis ,Lymph nodes metastases ,Distant metastasis ,TNM stage ,Diseases of the digestive system. Gastroenterology ,RC799-869 - Abstract
Abstract Background Gallbladder carcinoma (GBC) was the most common malignancy of biliary tract. Patients with malignancies frequently present with activated coagulation pathways, which might potentially related to tumor progression and prognosis. The purpose of the study was to investigate the clinical significance of preoperative serum fibrinogen levels and platelet counts in GBC patients. Methods The preoperative fasting serum fibrinogen levels and platelet counts of 58 patients with GBC were measured by AUV2700 automatic biochemical analyzer, as well as 60 patients with cholesterol polyps and 60 healthy volunteers. Kaplan–Meier survival analysis was applied to show the correction between fibrinogen levels and outcome after surgery. Results The fibrinogen levels of patients with GBC were significantly higher than healthy gallbladder and cholesterol polyp of gallbladder (p 0.05) in multivariate analysis. Conclusions The preoperative serum fibrinogen levels and platelet counts might be reliable biomarkers for the occurance of disease, tumor depth, lymph node metastasis, distant metastasis and advanced TNM stage in patients with GBC. The serum fibrinogen levels might be a prognostic factor to predict outcome for GBC patients suffering from surgery treatment. Anticoagulation therapy might be considered to control cancer progression in future studies.
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- 2021
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13. Association Between Maternal Exposure to SO2 and Congenital Ear Malformations in Offspring: A Population-Based Case-Control Study in Liaoning Province, China
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Wei-Jun Yu, Na Li, Ting-Ting Gong, Jia-Yu Zhang, Yu-Ting Jiang, Yu-Hong Zhao, Yan-Hong Huang, Jing Li, Shu Liu, Yan-Ling Chen, Li-Li Li, Cheng-Zhi Jiang, Zong-Jiao Chen, and Qi-Jun Wu
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air pollution ,congenital ear malformations ,microtia ,risk ,sulfur dioxide ,Public aspects of medicine ,RA1-1270 - Abstract
Objectives: To examine associations between maternal sulfur dioxide (SO2) exposure and congenital ear malformations risk in offspring.Methods: We surveyed 1676 cases with congenital ear malformations and 7950 controls from the Maternal and Child Health Certificate Registry of Liaoning Province between 2010 and 2015. SO2 concentrations were obtained from the Municipal Environment Protection Bureau of Liaoning Province. Multivariable logistic regression models and Restricted cubic splines (RCS) model were used to assess the aforementioned association.Results: There were significant associations between maternal SO2 exposure and congenital ear malformations risk during the 3 months before conception (OR Q4 vs. Q1 = 1.93, 95% CI = 1.43–2.59) and the 3 months after conception (OR Q4 vs. Q1 = 1.63, 95% CI = 1.22–2.18). Similar results were obtained in the analysis of single-month exposure windows, except for the third month before conception and the third month after conception. Moreover, these findings were broadly consistent across subgroups and robust in sensitivity analyses. There were non-linear dose-response associations between SO2 exposure and congenital ear malformations based on restricted cubic spline model analysis.Conclusion: Maternal SO2 exposure is associated with increased congenital ear malformations risk in offspring.
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- 2022
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14. Deep Learning for Approaching Hepatocellular Carcinoma Ultrasound Screening Dilemma: Identification of α-Fetoprotein-Negative Hepatocellular Carcinoma From Focal Liver Lesion Found in High-Risk Patients
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Wei-bin Zhang, Si-ze Hou, Yan-ling Chen, Feng Mao, Yi Dong, Jian-gang Chen, and Wen-ping Wang
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deep learning ,ultrasound ,AFP negative ,hepatocellular carcinoma ,focal liver lesion ,focal nodular hyperplasia ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
BackgroundFirst-line surveillance on hepatitis B virus (HBV)-infected populations with B-mode ultrasound is relatively limited to identifying hepatocellular carcinoma (HCC) without elevated α-fetoprotein (AFP). To improve the present HCC surveillance strategy, the state of the art of artificial intelligence (AI), a deep learning (DL) approach, is proposed to assist in the diagnosis of a focal liver lesion (FLL) in HBV-infected liver background.MethodsOur proposed deep learning model was based on B-mode ultrasound images of surgery that proved 209 HCC and 198 focal nodular hyperplasia (FNH) cases with 413 lesions. The model cohort and test cohort were set at a ratio of 3:1, in which the test cohort was composed of AFP-negative HBV-infected cases. Four additional deep learning models (MobileNet, Resnet50, DenseNet121, and InceptionV3) were also constructed as comparative baselines. To evaluate the models in terms of diagnostic power, sensitivity, specificity, accuracy, confusion matrix, F1-score, and area under the receiver operating characteristic curve (AUC) were calculated in the test cohort.ResultsThe AUC of our model, Xception, achieved 93.68% in the test cohort, superior to other baselines (89.06%, 85.67%, 83.94%, and 78.13% respectively for MobileNet, Resnet50, DenseNet121, and InceptionV3). In terms of diagnostic power, our model showed sensitivity, specificity, accuracy, and F1-score of 96.08%, 76.92%, 86.41%, and 87.50%, respectively, and PPV, NPV, FPR, and FNR calculated from the confusion matrix were respectively 80.33%, 95.24%, 23.08%, and 3.92% in identifying AFP-negative HCC from HBV-infected FLL cases. Satisfactory robustness of our proposed model was shown based on 5-fold cross-validation performed among the models above.ConclusionsOur DL approach has great potential to assist B-mode ultrasound in identifying AFP-negative HCC from FLL found in surveillance of HBV-infected patients.
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- 2022
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15. Maternal Exposure to Sulfur Dioxide and Risk of Omphalocele in Liaoning Province, China: A Population-Based Case-Control Study
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Li-Li Li, Yan-Hong Huang, Jing Li, Shu Liu, Yan-Ling Chen, Cheng-Zhi Jiang, Zong-Jiao Chen, and Yan-Yan Zhuang
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air pollution ,case-control study ,omphalocele ,risk ,sulfur dioxide ,Public aspects of medicine ,RA1-1270 - Abstract
Evidence of the association between maternal sulfur dioxide (SO2) exposure and the risk of omphalocele is limited and equivocal. We aimed to assess the aforementioned topic during the first trimester of pregnancy. A population-based case-control study was carried out in infants consisting of 292 cases of omphalocele and 7,950 healthy infant controls. Exposure to SO2, particulate matter with aerodynamic diameters ≤ 10 μm, and nitrogen dioxide was assessed by averaging the concentration from all stations in the mother's residential city. SO2 exposure was categorized into three groups, with the lowest tertile defined as the reference category. Odds ratios (ORs) and 95% confidence intervals (CIs) were estimated using multivariable logistic regression models. Higher SO2 exposure during the first trimester was significantly associated with omphalocele risk [per standard deviation (42 ug/m3) increment: OR = 1.39, 95% CI = 1.22–1.65]. When focusing on shorter exposure windows, similar positive associations were observed for SO2 exposure in the first and third months of pregnancy. In addition, compared with the lowest tertile, high SO2 exposure in the second month of pregnancy increased the risk of omphalocele (OR = 2.80, 95% CI = 1.61–4.97). Maternal exposure to SO2 during the first trimester may increase the risk of omphalocele in offspring.
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- 2022
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16. Prognostic value of low microRNA-34a expression in human gastrointestinal cancer: a systematic review and meta-analysis
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Yan-Ling Chen, Xiao-Lin Liu, and Ling Li
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microRNA-34a ,Gastrointestinal cancer ,Prognosis ,Meta-analysis ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Mounting evidence shows that microRNA-34a (miR-34a) is involved in cancer prognosis. Therefore, we summarize the predictive role of miR-34a for survival in patients with gastrointestinal cancers (GICs). Methods All eligible studies were found by searching PubMed, Web of Science and EMBASE, and survival results were extracted. Then, the hazard ratio (HR) with the corresponding 95% confidence interval (CI) was calculated to evaluate the prognostic role of miR-34a in GICs. The association between miR-34a expression and clinicopathological characteristics was estimated by odds ratios (ORs) and 95% CIs. Results A total of 20 studies were included in this meta-analysis. For overall survival (OS), lower miR-34a expression could probably predict poorer outcome in GICs, with a pooled HR of 1.86 (95% CI: 1.52–2.28, P
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- 2021
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17. The BRD4 Inhibitor dBET57 Exerts Anticancer Effects by Targeting Superenhancer-Related Genes in Neuroblastoma
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Si-Qi Jia, Ran Zhuo, Zi-Mu Zhang, Yang Yang, Yan-Fang Tao, Jian-Wei Wang, Xiao-Lu Li, Yi Xie, Gen Li, Di Wu, Yan-Ling Chen, Juan-Juan Yu, Chen-xi Feng, Zhi-Heng Li, Rong-Fang Zhou, Ran-Dong Yang, Peng-Cheng Yang, Bi Zhou, Xiao-Mei Wan, Yu-Meng Wu, Wan-Yan Jiao, Ni-Na Zhou, Fang Fang, and Jian Pan
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Immunologic diseases. Allergy ,RC581-607 - Abstract
Neuroblastoma (NB) is the most common solid tumor of the neural crest cell origin in children and has a poor prognosis in high-risk patients. The oncogene MYCN was found to be amplified at extremely high levels in approximately 20% of neuroblastoma cases. In recent years, research on the targeted hydrolysis of BRD4 to indirectly inhibit the transcription of the MYCN created by proteolysis targeting chimaera (PROTAC) technology has become very popular. dBET57 (S0137, Selleck, TX, USA) is a novel and potent heterobifunctional small molecule degrader based on PROTAC technology. The purpose of this study was to investigate the therapeutic effect of dBET57 in NB and its potential mechanism. In this study, we found that dBET57 can target BRD4 ubiquitination and disrupt the proliferation ability of NB cells. At the same time, dBET57 can also induce apoptosis, cell cycle arrest, and decrease migration. Furthermore, dBET57 also has a strong antiproliferation function in xenograft tumor models in vivo. In terms of mechanism, dBET57 targets the BET protein family and the MYCN protein family by associating with CRBN and destroys the SE landscape of NB cells. Combined with RNA-seq and ChIP-seq public database analysis, we identified the superenhancer-related genes TBX3 and ZMYND8 in NB as potential downstream targets of dBET57 and experimentally verified that they play an important role in the occurrence and development of NB. In conclusion, these results suggest that dBET57 may be an effective new therapeutic drug for the treatment of NB.
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- 2022
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18. Development and validation of novel nomograms for predicting the survival of patients after surgical resection of pancreatic ductal adenocarcinoma
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Ge Li, Jiang‐Zhi Chen, Shi Chen, Sheng‐Zhe Lin, Wei Pan, Ze‐Wu Meng, Xin‐Ran Cai, and Yan‐Ling Chen
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cancer‐specific survival ,decision curve analysis ,nomogram ,overall survival ,pancreatic ductal adenocarcinoma ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background/Aims Pancreatic ductal adenocarcinoma (PDAC) is associated with high mortality, even after surgical resection. The existing predictive models for survival have limitations. This study aimed to develop better nomograms for predicting overall survival (OS) and cancer‐specific survival (CSS) in PDAC patients after surgery. Methods A total of 6323 PDAC patients were retrospectively recruited from the Surveillance, Epidemiology, and End Results (SEER) database and randomly allocated into training, validation, and test cohorts. Multivariate Cox regression analysis was conducted to identify significant independent factors for OS and CSS, which were used for construction of nomograms. The performance was evaluated, validated, and compared with that of the 8th edition AJCC staging system. Results Ten independent factors were significantly correlated with OS and CSS. The 1‐, 3‐, and 5‐year OS rates were 40%, 20%, and 15%, and 1‐, 3‐, and 5‐year CSS rates were 45%, 24%, and 19%, respectively. The nomograms were calibrated well, with c‐indexes of 0.640 for OS and 0.643 for CSS, respectively. Notably, relative to the 8th edition AJCC staging system, the nomograms were able to stratify each AJCC stage into three prognostic subgroups for more robust risk stratification. Furthermore, the nomograms achieved significant clinical validity, exhibiting wide threshold probabilities and high net benefit. Performance assessment also showed high predictive accuracy and reliability. Conclusions The predictive ability and reliability of the established nomograms have been validated, and therefore, these nomograms hold potential as novel approaches to predicting survival and assessing survival risks for PDAC patients after surgery.
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- 2020
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19. Effects and Mechanism of Salvianolic Acid B on the Injury of Human Renal Tubular Epithelial Cells Induced by Iopromide
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Shu-Jun Dong, Xin-Yue Gao, Ming-Xin Pei, Ting Luo, Dong Fan, Yan-Ling Chen, Jun-Feng Jin, and Xiao-Duo Zhao
- Subjects
salvianolic acid B ,iopromide ,contrast-induced nephropathy ,endoplasmic reticulum stress ,HK-2 cell ,Therapeutics. Pharmacology ,RM1-950 - Abstract
With the increasing application of medical imaging contrast materials, contrast-induced nephropathy (CIN) has become the third major cause of iatrogenic renal insufficiency. CIN is defined as an absolute increase in serum creatinine levels of at least 0.50 mg/dl or an increase >25% of serum creatinine from baseline after exposure to contrast. In this study, the protective effects of salvianolic acid B (Sal B) were detected in human renal tubular epithelial cells (HK-2) exposed to iopromide. The results showed that different concentrations of Sal B counteract the loss of cell viability induced by iopromide, and reduce cell apoptosis, the reactive oxygen species (ROS) levels, and the levels of endoplasmic reticulum stress (ERS)–related and apoptosis-related proteins such as p-IRE-1α, p-eIF-2α/eIF-2α, p-JNK, CHOP, Bax/Bcl-2, and cleaved caspase-3. In addition, Sal B at a concentration of 100 μmol/L inhibited ERS and reduced cell damage to a similar extent as the ERS inhibitor 4-PBA. Importantly, treatment with Sal B could abolish the injury induced by ERS agonist tunicamycin, increasing cell viability and the mitochondrial membrane potential, as well as significantly reducing ROS levels and the expression of Bax/Bcl-2, cleaved-caspase-3, GRP78, p-eIF2α, p-JNK, and CHOP. These results suggested that the protective effect of Sal B against HK-2 cell injury induced by iopromide may be related to the inhibition of ERS.
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- 2021
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20. Maternal PM10 Exposure Increases Risk for Spina Bifida: A Population-Based Case-Control Study
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Huan Li, Yan-Hong Huang, Jing Li, Shu Liu, Yan-Ling Chen, Li-Li Li, Cheng-Zhi Jiang, Zong-Jiao Chen, and Na Li
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PM10 ,spina bifida ,birth defects ,air pollution ,particulate matter ,case-control study ,Public aspects of medicine ,RA1-1270 - Abstract
Limited studies have focused on the impact of ambient air pollution on spina bifida. A population-based case-control study was conducted in Liaoning Province, China to assess the associations between maternal PM10 exposures in various exposure windows and spina bifida risk. Data on spina bifida cases born between 2010 and 2015 were available from the Maternal and Child Health Certificate Registry of Liaoning Province. Controls were a random sample of healthy livebirths without any birth defects delivered in the selected five cities during 2010–2015. Ambient air monitoring data for PM10 were obtained from 75 monitoring stations in Liaoning Province. The multivariable logistic regression models were established to calculate adjusted odds ratios (OR) and 95% confidence intervals (CI). We further performed sensitivity analyses by using three propensity score methods. A total of 749 spina bifida cases and 7,950 controls were included. After adjusting for potential confounders, spina bifida was associated with a 10 μg/m3 increment in PM10 during the first trimester of pregnancy (adjusted OR = 1.06, 95% CI: 1.00–1.12) and the 3 months before pregnancy (adjusted OR = 1.12, 95% CI: 1.06–1.19). The adjusted ORs in the final model for the highest vs. the lowest quartile were 1.51 (95% CI: 1.04–2.19) for PM10 during the first trimester of pregnancy and 2.01 (95% CI: 1.43–2.81) for PM10 during the 3 months before pregnancy. Positive associations were found between PM10 exposures during the single month exposure windows and spina bifida. Sensitivity analyses based on two propensity score methods largely reported similar positive associations. Our findings support the evidence that maternal PM10 exposure increases the risk of spina bifida in offspring. Further, validation with a prospective design and a more accurate exposure assessment is warranted.
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- 2021
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21. Construction and Validation of Novel Nomograms for Predicting Prognosis of Pancreatic Ductal Adenocarcinoma After Surgery According to Different Primary Cancer Locations
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Ge Li, Cheng-Yu Liao, Jiang-Zhi Chen, Long Huang, Can Yang, Yi-Feng Tian, Yi-Ting Wang, Qiang Du, Qian Zhan, Yan-Ling Chen, and Shi Chen
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pancreatic ductal adenocarcinoma ,nomogram ,cancer-specific survival (CSS) ,decision curve analysis ,AJCC 8 ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background/AimsPancreatic ductal adenocarcinoma (PDAC) can occur in different parts of the pancreas. This study aimed to identify clinicopathological characteristics independently correlated with the prognosis of PDAC of the pancreatic head/uncinate (PHC) or body-tail (PBTC), and to develop novel nomograms for predicting cancer-specific survival (CSS) according to different primary cancer locations.Methods1160 PDAC patients were retrospectively enrolled and assigned to training and test sets with each set divided into PHC and PBTC groups. Comparative analysis of clinicopathologic characteristics, survival analysis, and multivariate analysis were performed. Independent factors were identified and used for constructing nomograms. The performance of the nomograms was validated in the test set.ResultsPrimary tumor location was an independent risk factor for prognosis of PDAC after surgery. Specially, gender, fasting blood glucose, and preoperative cancer antigen 19-9 were significantly associated with prognosis of PHC, whereas age, body mass index, and lymph nodes were significantly correlated with the prognosis of PBTC. A significant difference in prognosis was found between PHC and PBTC in stage Ia and stage III. Three nomograms were established for predicting the prognosis for PDAC, PHC, and PBTC. Notably, these nomograms were calibrated modestly (c-indexes of 0.690 for PDAC, 0.669 for PHC, and 0.704 for PBTC), presented better accuracy and reliability than the 8th AJCC staging system, and achieved clinical validity.ConclusionsPHC and PBTC share the differential clinical-pathological characteristics and survival. The nomograms show good performance for predicting prognosis in PHC and PBTC. Therefore, these nomograms hold potential as novel approaches for predicting survival of PHC and PBTC patients after surgery.
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- 2021
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22. In situ preparation of well-dispersed CuO nanocatalysts in heavy oil for catalytic aquathermolysis
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Ming Chen, Chen Li, Guo-Rui Li, Yan-Ling Chen, and Cheng-Gang Zhou
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Cu(OH)2-contained microemulsion ,In situ preparation ,CuO nanocatalyst ,Catalytic viscosity reduction ,Heavy oil ,Science ,Petrology ,QE420-499 - Abstract
Abstract We developed an in situ synthesis strategy for preparing well-dispersed CuO nanoparticles as aquathermolysis catalyst for viscosity reduction in Shengli heavy oil (China). A Cu(OH)2-contained microemulsion was employed as a carrier to disperse the precursor Cu(OH)2 to the heavy oil phase. Under aquathermolysis condition (240 °C, 2.5 MPa of N2), the Cu(OH)2 precursors would first be converted in situ to well-crystallized and size-homogeneous CuO nanoparticles naturally, catalyzed by which the viscosity of Shengli heavy oil could be reduced as much as 94.6%; simultaneously, 22.4% of asphaltenes were converted to light components. The agglomeration of the in situ prepared monoclinic CuO nanoparticles could be negligible throughout the catalytic reaction. Based on the characterization results of 1H NMR, elemental analysis and GC–MS of oil samples before and after catalytic aquathermolysis, the mechanism for viscosity reduction of heavy oil in the catalytic system was investigated.
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- 2019
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23. The Application of a Bone Marrow Mesenchymal Stem Cell Membrane in the Vascularization of a Decellularized Tracheal Scaffold
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Zhi-Ye Yao, Bo-Wen Feng, Cai-Sheng Liu, Yu-Mei Liu, Hai-Yu Zhou, Xiao-Hui Zhang, Min-Qiao Jian, Jian-Ling Mo, Yi-Jing Liang, Liang Chen, Xiao-Qing Liu, Yan-Ling Chen, Zhan-Song Zhang, and Shao-Ru He
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Internal medicine ,RC31-1245 - Abstract
Airway stenosis is a common problem in the neonatal intensive care unit (NICU) and pediatric intensive care unit (PICU). A tissue-engineered trachea is a new therapeutic method and a research hotspot. Successful vascularization is the key to the application of a tissue-engineered trachea. However, successful vascularization studies lack a complete description. In this study, it was assumed that rabbit bone marrow mesenchymal stem cells were obtained and induced by ascorbic acid to detect the tissue structure, ultrastructure, and gene expression of the extracellular matrix. A vascular endothelial cell culture medium was added in vitro to induce the vascularization of the stem cell sheet (SCS), and the immunohistochemistry and gene expression of vascular endothelial cell markers were detected. At the same time, vascular growth-related factors were added and detected during SCS construction. After the SCS and decellularized tracheal (DT) were constructed, a tetrandrine allograft was performed to observe its vascularization potential. We established the architecture and identified rabbit bone marrow mesenchymal stem cell membranes by 14 days of ascorbic acid, studied the role of a vascularized membrane in inducing bone marrow mesenchymal stem cells by in vitro ascorbic acid, and assessed the role of combining the stem cell membranes and noncellular tracheal scaffolds in vivo. Fourteen experiments confirmed that cell membranes promote angiogenesis at gene level. The results of 21-day in vitro experiments showed that the composite tissue-engineered trachea had strong angiogenesis. In vivo experiments show that a composite tissue-engineered trachea has strong potential for angiogenesis. It promotes the understanding of diseases of airway stenosis and tissue-engineered tracheal regeneration in newborns and small infants.
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- 2021
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24. Assessment of the practicability of implementing the EULAR recommendations for the role of nurses in the management of chronic inflammatory arthritis in China
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Ling Ma, Yan Liang, Sheng-nan Yu, Ying Wang, Susan M Oliver, Yan-ling Chen, Yi Zhao, Yu-qiong Cao, Xue-mei Liu, Zi-yun Zhang, Li-hong Chen, and Yi Liu
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Medicine - Abstract
Chronic inflammatory arthritis (CIA) severely impacts quality of life in over 100 million people in China. In 2011, the European League Against Rheumatism (EULAR) developed recommendations for the role of nurses in the management of CIA. However, it remains unknown whether these recommendations could be fully implemented in China. Therefore, we aimed to solve the problem in this study. We conducted a nationwide cross-sectional study among 485 nurses in rheumatology and immunology departments based on an online questionnaire. The agreement of the recommendations by the subjects and the feasibility of the recommendations they believe were assessed by visual analogue scale (VAS). Our results showed that over 80% of the subjects agreed with each recommendation (VAS 5–10), and over 80% of the subjects considered the recommendations feasible (VAS 5–10). This study indicates that the EULAR recommendations can also be well implemented in China.
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- 2020
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25. A study on the epidemiological characteristics and infectious forecast model of malaria at Guangzhou Airport among Chinese returnees from Africa
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Hui-ming Wu, Zhi-qiang Fang, Dang Zhao, Yan-ling Chen, Chuan-ge Liu, and Xi Liang
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Chinese travellers ,Africa ,Malaria ,Epidemiological characteristics ,Forecast model ,Arctic medicine. Tropical medicine ,RC955-962 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Cross-border malaria transmission in China is a major component of Chinese imported malaria cases. Such cases mostly are travellers returning from malaria endemic countries in Africa. By investigating malaria infectious status among Chinese worker in Africa, this study analysed the malaria risk factors, in order to establish infectious forecast model. Methods Chinese returnees data from Africa were collected at Guangzhou Baiyun International Airport, Guangzhou, China between August 2015 and March 2016 and were included in the cross-sectional and retrospective survey. Results A total of 1492 respondents were included in the study with the majority consisting of junior middle school educated male. Most of them are manual and technical workers hired by companies, with average of 37.04 years of age. Overall malaria incidence rate of the population was 8.98% (134/1492), and there were no significant differences regarding age, gender, occupation, or team. Forecast model was developed on the basis of malaria risk factors including working country, local ecological environment type, work duration and intensity of mosquito bite prevention. Conclusions The survey suggested that malaria incidence was high among Chinese travellers who had worked in Africa countries of heavy malaria burden. Further research on the frequency and severity of clinical episodes among Chinese travellers having worked in Africa is needed.
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- 2017
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26. Clinical significance of elevated serum citrulline levels in patients with epilepsy
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Yan ZHANG, Yan-ling CHEN, Mei-mei HAO, Na QIN, and Yan-chun DENG
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Epilepsy ,Citrullinemia ,Anticonvulsants ,Mass spectrometry ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Objective To discuss the clinical significance of elevated serum citrulline levels in patients with epilepsy, and to provide new idea for improving the curative effect of antiepileptic drugs (AEDs). Methods Select 159 child and adolescent patients with cryptogenic epilepsy but without brain structural damage, screen serum concentration of citrulline in these patients by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and perform citrulline metabolism-related gene testing, including SLC25A13 gene and ASS1 gene, in part of those patients with a higher citrulline level, in order to identify the etiology of epilepsy. Results Among 159 patients, the citrulline levels of 18 cases were searched for 1.53-3.61 times higher than normal, and no causative mutation or novel mutation of SLC25A13 gene and ASS1 gene was found in 6 cases who were performed gene testing. AEDs were given to those 18 patients for at least one year. Seven cases (7/18) were free of clinical seizures, and 3 cases (3/18) had at least 75% reduction in seizure frequency, 3 cases (3/18) 50%-75% reduction in seizure frequency, 5 cases (5/18) seizure reduction below 50% . Compared with 30 cases with normal citrulline level (control group), the efficacy of AEDs in those 18 cases were significantly lower (Z = 1.759, P = 0.039). There was no significant difference between these 2 groups in basic features including sex, onset age, duration of seizure, seizure type, seizure frequency and kind of AEDs. Conclusions Higher citrulline level (1.53-3.61 times) may lead to a negative effect on the efficacy of AEDs of child and adolescent patients with cryptogenic epilepsy in this study. doi: 10.3969/j.issn.1672-6731.2014.11.013
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- 2014
27. Expression and prognostic value of soluble CD97 and its ligand CD55 in intrahepatic cholangiocarcinoma
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Ze-Wu Meng, Min-Chao Liu, Hai-Jie Hong, Qiang Du, and Yan-Ling Chen
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
The incidence rate of intrahepatic cholangiocarcinoma is rising, and treatment options are limited. Therefore, new biological markers of intrahepatic cholangiocarcinoma are needed. Immunohistochemistry and enzyme-linked immunosorbent assay were applied to analyze the expressions of CD97, CD55, and soluble CD97 in 71 patients with intrahepatic cholangiocarcinoma and 10 patients with hepatolithiasis. CD97 and CD55 were not expressed in hepatolithiatic tissues, but positive expression was observed in 76.1% (54/71) and 70.4% (50/71) of intrahepatic cholangiocarcinoma patients. The univariate analyses indicated that the positive expressions of CD97 and CD55 were related to short intrahepatic cholangiocarcinoma survival of patients (both p = 0.001). Furthermore, CD97 and CD55 expressions and biliary soluble CD97 levels were significantly associated with histological grade (p = 0.004, 0.002, and 0.012, respectively), lymph node metastases (p = 0.020, 0.038, and 0.001, respectively), and venous invasion (p = 0.003, 0.002, and 0.001, respectively). The multivariate analyses indicated that lymph node metastases (hazard ratio: 2.407, p = 0.003), positive CD55 expression (hazard ratio: 4.096, p = 0.003), and biliary soluble CD97 levels (hazard ratio: 2.434, p = 0.002) were independent risk factors for the intrahepatic cholangiocarcinoma survival. The receiver operating characteristic (ROC) curve analysis indicated that when the cutoff values of biliary soluble CD97 were 1.15 U/mL, the diagnostic value for predicting lymph node metastasis had a sensitivity of 87.5% and a specificity of 51.3%. For intrahepatic cholangiocarcinoma patient death within 60 months at a cutoff value of 0.940 U/mL, the diagnostic value sensitivity was 89.3% and the specificity was 93.3%. Biliary soluble CD97 may be a new biological marker for early diagnosis, prediction of lymph node metastasis and poor prognosis, and discovery of a therapeutic target.
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- 2017
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28. Pyruvate kinase M2 plays a dual role on regulation of the EGF/EGFR signaling via E-cadherin-dependent manner in gastric cancer cells.
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Le-Yi Wang, Yun-Peng Liu, Li-Gang Chen, Yan-Ling Chen, Li Tan, Jing-Jing Liu, Amarsanaa Jazag, Jian-Lin Ren, and Bayasi Guleng
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Medicine ,Science - Abstract
BACKGROUND AND AIMS: EGFR activation and PKM2 expression are instrumental in tumorigenesis. EGFR activation regulates PKM2 functions in a subcellular compartment-dependent manner and promotes gene transcription and tumor growth. In addition, PKM2 is upregulated in EGFR-induced pathways in glioma malignancies. However, we found that PKM2 could also regulate the activity of the EGF/EGFR signaling pathway in gastric cancer cells. We aimed to define the biological mechanisms for PKM2 in regulating the cell motility and invasion. METHODS: We employed stable transfection with short hairpin RNA to stably silence the expression of PKM2 in the BGC823, SGC7901 and AGS gastric cancer cell lines. The effects of PKM2 in vitro were determined by assessing cell migration and invasion. Immunohistochemical analysis was used to explore the relationship among PKM2 and other proteins. RESULTS: Our results indicate that the knockdown of PKM2 decreased the activity of E-cadherin and enhanced the EGF/EGFR signaling pathway in the gastric cell lines BGC823 and SGC7901 that were positive for E-cadherin expression. However, in the undifferentiated gastric carcinoma cell line AGS, which lacks E-cadherin expression, PKM2 promoted cell migration and invasion. Immunohistochemical analyses showed that the levels of E-cadherin expression, ERK1/2 phosphorylation, and cytoplasmic PKM2 expression were correlated with each other. CONCLUSION: PKM2 may play different roles in differently differentiated gastric cancer cell types, and this finding would be consistent with the previous clinical research. The results of our study reveal an important link between PKM2 and E-cadherin during EGFR-stimulated gastric cancer cell motility and invasion.
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- 2013
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29. Preliminary Modeling of Gender Identification in the Social Community.
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Kuo-Chung Chu, Peng-Hua Jiang, Huan-Yu Syu, and Yan-Ling Chen
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- 2018
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30. Value of red blood cell distribution width in prediction of diastolic dysfunction in cirrhotic cardiomyopathy
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Yan-Ling Chen, Zi-Wen Zhao, Shu-Mei Li, and Yong-Zhe Guo
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Gastroenterology ,General Medicine - Published
- 2023
31. Infestation and distribution of chigger mites on Confucian white-bellied rat (Niviventer confucianus) in Southwest China
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Yan-Ling Chen, Xian-Guo Guo, Wen-Yu Song, Tian-Guang Ren, Lei Zhang, Rong Fan, Cheng-Fu Zhao, Zhi-Wei Zhang, Wen-Ge Dong, and Xiao-Bin Huang
- Subjects
Genetics ,Animal Science and Zoology ,Cell Biology ,Plant Science ,Molecular Biology ,Biochemistry ,Ecology, Evolution, Behavior and Systematics - Published
- 2022
32. Pachymic acid modulates sirtuin 6 activity to alleviate lipid metabolism disorders
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Zhi-Sen Pan, Yan-Ling Chen, Kai-Jia Tang, Zhang-Zhou Liu, Jia-Li Liang, Yan-Hao Guan, Xiao-Yi Xin, Chang-Hui Liu, and Chuang-Peng Shen
- Subjects
Cancer Research ,Immunology and Microbiology (miscellaneous) ,General Medicine - Published
- 2023
33. Supplementary Figure Legend from CDC25B Mediates Rapamycin-Induced Oncogenic Responses in Cancer Cells
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Jiing-Dwan Lee, John R. Yates, Colleen Fearns, Yan-ling Chen, Greg Cantin, Wei Yi, Bing-wen Lu, Qing-kai Yang, and Run-qiang Chen
- Abstract
Supplementary Figure Legend from CDC25B Mediates Rapamycin-Induced Oncogenic Responses in Cancer Cells
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- 2023
34. Supplementary Figure 1 from CDC25B Mediates Rapamycin-Induced Oncogenic Responses in Cancer Cells
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Jiing-Dwan Lee, John R. Yates, Colleen Fearns, Yan-ling Chen, Greg Cantin, Wei Yi, Bing-wen Lu, Qing-kai Yang, and Run-qiang Chen
- Abstract
Supplementary Figure 1 from CDC25B Mediates Rapamycin-Induced Oncogenic Responses in Cancer Cells
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- 2023
35. Data from CDC25B Mediates Rapamycin-Induced Oncogenic Responses in Cancer Cells
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Jiing-Dwan Lee, John R. Yates, Colleen Fearns, Yan-ling Chen, Greg Cantin, Wei Yi, Bing-wen Lu, Qing-kai Yang, and Run-qiang Chen
- Abstract
Because the mammalian target of rapamycin (mTOR) pathway is commonly deregulated in human cancer, mTOR inhibitors, rapamycin and its derivatives, are being actively tested in cancer clinical trials. Clinical updates indicate that the anticancer effect of these drugs is limited, perhaps due to rapamycin-dependent induction of oncogenic cascades by an as yet unclear mechanism. As such, we investigated rapamycin-dependent phosphoproteomics and discovered that 250 phosphosites in 161 cellular proteins were sensitive to rapamycin. Among these, rapamycin regulated four kinases and four phosphatases. A siRNA-dependent screen of these proteins showed that AKT induction by rapamycin was attenuated by depleting cellular CDC25B phosphatase. Rapamycin induces the phosphorylation of CDC25B at Serine375, and mutating this site to Alanine substantially reduced CDC25B phosphatase activity. Additionally, expression of CDC25B (S375A) inhibited the AKT activation by rapamycin, indicating that phosphorylation of CDC25B is critical for CDC25B activity and its ability to transduce rapamycin-induced oncogenic AKT activity. Importantly, we also found that CDC25B depletion in various cancer cell lines enhanced the anticancer effect of rapamycin. Together, using rapamycin phosphoproteomics, we not only advance the global mechanistic understanding of the action of rapamycin but also show that CDC25B may serve as a drug target for improving mTOR-targeted cancer therapies. [Cancer Res 2009;69(6):OF2663–8]
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- 2023
36. Supplementary Table 1 from CDC25B Mediates Rapamycin-Induced Oncogenic Responses in Cancer Cells
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Jiing-Dwan Lee, John R. Yates, Colleen Fearns, Yan-ling Chen, Greg Cantin, Wei Yi, Bing-wen Lu, Qing-kai Yang, and Run-qiang Chen
- Abstract
Supplementary Table 1 from CDC25B Mediates Rapamycin-Induced Oncogenic Responses in Cancer Cells
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- 2023
37. Supplementary Table 2 from CDC25B Mediates Rapamycin-Induced Oncogenic Responses in Cancer Cells
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Jiing-Dwan Lee, John R. Yates, Colleen Fearns, Yan-ling Chen, Greg Cantin, Wei Yi, Bing-wen Lu, Qing-kai Yang, and Run-qiang Chen
- Abstract
Supplementary Table 2 from CDC25B Mediates Rapamycin-Induced Oncogenic Responses in Cancer Cells
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- 2023
38. Disease vector occurrence and ecological characteristics of chiggers on the chestnut white-bellied rat Niviventer fulvescens in Southwest China between 2001 and 2019.
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Yan-Ling Chen, Xian-Guo Guo, Wen-Yu Song, Tian-Guang Ren, Lei Zhang, Rong Fan, Cheng-Fu Zhao, Zhi-Wei Zhang, Wen-Ge Dong, Xiao-Bin Huang, and Dao-Chao Jin
- Subjects
TSUTSUGAMUSHI disease ,CHESTNUT ,MICROSCOPES ,LEPTOTROMBIDIUM - Abstract
Chigger mites are the vector of scrub typhus. This study estimates the infestation status and ecological characteristics of chiggers on the chestnut white-bellied rat Niviventer fulvescens in Southwest China between 2001 and 2019. Chiggers were identified under the microscope, and infestation indices were calculated. The Preston’s log-normal model was used to fit the curve of species abundance distribution. A total of 6,557 chiggers were collected in 136 of 342 N. fulvescens rats, showing high overall infestation indices (prevalence=39.8%, mean abundance=19.2, mean intensity=48.2) and high species diversity (S=100, H’=3.0). Leptotrombidium cangjiangense, Neotrombicula japonica, and Ascoschoengastia sifanga were the three dominant chigger species (constituent ratio=42.9%; 2,736/6,384) and exhibited an aggregated distribution among different rat individuals. We identified 100 chigger species, with 3 of them (Leptotrombidium scutellare, Leptotrombidium wenense, and Leptotrombidium deliense) as the main vectors of scrub typhus in China and nine species as potential vectors of this disease. Disease vector occurrence on N. fulvescens may increase the risk of spreading scrub typhus from rats to humans. Chigger infestation on N. fulvescens varied significantly in different environments. The species abundance distribution showed a log-normal distribution pattern. The estimated number of chigger species on N. fulvescens was 126 species. [ABSTRACT FROM AUTHOR]
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- 2023
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39. A Report of Chigger Mites on the Striped Field Mouse, Apodemus agrarius, in Southwest China
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Yan-Ling Chen, Xian-Guo Guo, Tian-Guang Ren, Lei Zhang, Rong Fan, Cheng-Fu Zhao, Zhi-Wei Zhang, Ke-Yu Mao, Xiao-Bin Huang, and Ti-Jun Qian
- Subjects
Male ,Apodemus agrarius ,China ,Mite Infestations ,Mites ,integumentary system ,southwest China ,chigger mite ,rodent ,virus diseases ,Trombiculidae ,ectoparasite ,Mice ,Infectious Diseases ,Scrub Typhus ,Animals ,Parasitology ,Original Article ,Female ,Murinae ,Acari - Abstract
Based on the field investigations in 91 investigation sites (counties) in southwest China between 2001 and 2019, the present paper reported the chigger mites on A. agrarius mice in southwest China for the first time by using a series of statistical methods. From 715 striped field mice captured in 28 of 91 investigated sites, only 255 chiggers were collected, and they were identified as 14 species, 6 genera in 3 subfamilies under 2 families. Of 715 A. agrarius mice, only 24 of them were infested with chigger mites with low overall prevalence (PM=3.4%), overall mean abundance (MA=0.36 mites/host) and overall mean intensity (MI=10.63 mites/host). The species diversity and infestation of chiggers on A. agrarius were much lower than those previously reported on some other rodents in southwest China. On a certain species of rodent, A. agrarius mouse in southwest China seems to have a very low susceptibility to chigger infestations than in other geographical regions. Of 14 chigger species, there were 3 dominant species, Leptotrombidium sialkotense, L. rupestre and Schoengastiella novoconfuciana, which were of aggregated distribution among different individuals of A. agrarius hosts. L. sialkotense, one of 6 main vectors of scrub typhus in China, was the first dominant on A. agrarius. The species similarity of chigger mites on male and female hosts was low with CSS=0.25, and this reflects the sex-bias of different genders of A. agrarius mice in harboring different chigger species.
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- 2021
40. CEBPG promotes acute myeloid leukemia progression by enhancing EIF4EBP1
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Si-Qi Jia, Ran Zuo, Xiao-Lu Li, Yi Xie, Shaoyan Hu, Gen Li, Zi-Mu Zhang, Jing-Jing Pan, Hai-Rong Wang, Xin-Mei Liao, You Jiang, Jian-Wei Wang, Hai-Bo Cao, Zheng Zhang, Juan-Juan Yu, Xinran Chu, Jian Pan, Jun Lu, Fang Fang, Chen-Xi Feng, Di Wu, Yan-Fang Tao, Yong-Ping Zhang, Yan-Ling Chen, Shuiyan Wu, and Zhi-Heng Li
- Subjects
Cancer Research ,Myeloid ,Proliferation ,Apoptosis ,Biology ,Myeloid Neoplasm ,Small hairpin RNA ,CEBPG ,hemic and lymphatic diseases ,Genetics ,medicine ,neoplasms ,RC254-282 ,EIF4EBP1 ,Gene knockdown ,Acute myeloid leukemia ,QH573-671 ,Cell growth ,Myeloid leukemia ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Haematopoiesis ,medicine.anatomical_structure ,Oncology ,Cancer research ,Primary Research ,Cytology - Abstract
Background Acute myeloid leukemia (AML) is a myeloid neoplasm accounts for 7.6% of hematopoietic malignancies. AML is a complex disease, and understanding its pathophysiology is contributing to the improvement in the treatment and prognosis of AML. In this study, we assessed the expression profile and molecular functions of CCAAT enhancer binding protein gamma (CEBPG), a gene implicated in myeloid differentiation and AML progression. Methods shRNA mediated gene interference was used to down-regulate the expression of CEBPG in AML cell lines, and knockdown efficiency was detected by RT-qPCR and western blotting. The effect of knockdown on the growth of AML cell lines was evaluated by CCK-8. Western blotting was used to detect PARP cleavage, and flow cytometry were used to determine the effect of knockdown on apoptosis of AML cells. Genes and pathways affected by knockdown of CEBPG were identified by gene expression analysis using RNA-seq. One of the genes affected by knockdown of CEBPG was Eukaryotic translation initiation factor 4E binding protein 1 (EIF4EBP1), a known repressor of translation. Knockdown of EIF4EBP1 was used to assess its potential role in AML progression downstream of CEBPG. Results We explored the ChIP-Seq data of AML cell lines and non-AML hematopoietic cells, and found CEBPG was activated through its distal enhancer in AML cell lines. Using the public transcriptomic dataset, the Cancer Cell Line Encyclopedia (CCLE) and western blotting, we also found CEBPG was overexpressed in AML. Moreover, we observed that CEBPG promotes AML cell proliferation by activating EIF4EBP1, thus contributing to the progression of AML. These findings indicate that CEBPG could act as a potential therapeutic target for AML patients. Conclusion In summary, we systematically explored the molecular characteristics of CEBPG in AML and identified CEBPG as a potential therapeutic target for AML patients. Our findings provide novel insights into the pathophysiology of AML and indicate a key role for CEBPG in promoting AML progression.
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- 2021
41. Two new dihydro
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Juan, Tang, Jia-Zhen, Hu, Yan-Ling, Chen, Ting-Ting, Zhao, Tao-Xiang, Chen, and Cheng-Gang, Hu
- Abstract
Two new dihydro
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- 2022
42. Using massMobile, a flexible, scalable, rapid prototyping audience participation framework, in large-scale live musical performances.
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Jason Freeman 0001, Shaoduo Xie, Takahiko Tsuchiya, Weibin Shen, Yan-Ling Chen, and Nathan Weitzner
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- 2015
- Full Text
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43. Hepatic abscess caused by esophageal foreign body misdiagnosed as cystadenocarcinoma by magnetic resonance imaging: A case report
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Ze-Wu Meng, Wei Pan, Xin-Ran Cai, Li-Jing Lin, and Yan-Ling Chen
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medicine.medical_specialty ,endocrine system diseases ,medicine.diagnostic_test ,business.industry ,Hepatic abscess ,Cystadenocarcinoma ,Esophageal foreign body ,Magnetic resonance imaging ,General Medicine ,equipment and supplies ,medicine.disease ,stomatognathic diseases ,Caudate lobe ,Case report ,medicine ,Radiology ,business ,human activities - Abstract
BACKGROUND Foreign bodies stuck in the throat and esophagus can be discharged through the digestive tract. Esophageal-lodged foreign bodies can cause secondary injury or detrimental response, with hepatic abscess being one such, albeit rare, outcome. Review and discussion of the few case reports on such instances will help to improve the overall understanding of such conditions and aid in differential diagnosis to improve patient outcome. CASE SUMMARY A 51-year-old female patient with pre-existing diabetes visited our hospital following a 15-d experience of chills and fever. Both plain and enhanced magnetic resonance imaging and color Doppler ultrasound examination of the liver and gallbladder revealed a space-occupying lesion in the caudate lobe of the liver (7.8 cm × 6.0 cm × 5.0 cm). Initially, a malignant tumor was suspected, but differential diagnosis was unable to exclude the possibility of hepatic abscess. Conservative anti-infection therapy produced a less than ideal outcome. Additional examination by hepatobiliary imaging with computed tomography suggested a foreign body present in the upper abdomen and hepatic abscess, and subsequent endoscopy revealed a sinus tract in the anterior wall of the duodenal bulb. Therefore, surgery was performed to remove the object (fishbone) and drain the abscess. After a 2-wk uneventful recovery, the patient was discharged. The final diagnosis was foreign body-induced hepatic abscess of the caudate lobe. CONCLUSION Differential diagnosis is important for hepatic masses, and systematic examination and physician awareness can aid in diagnosing and curing such rare conditions.
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- 2021
44. Textbook outcomes of hepatocellular carcinoma patients with sarcopenia: A multicenter analysis
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Di-Hang Wu, Cheng-Yu Liao, Dan-Feng Wang, Long Huang, Ge Li, Jiang-Zhi Chen, Liang Wang, Tian-Sheng Lin, Jian-Lin Lai, Song-Qiang Zhou, Fu-Nan Qiu, Zhi-Bo Zhang, Yan-Ling Chen, Yao-Dong Wang, Xiao-Chun Zheng, Yi-Feng Tian, and Shi Chen
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Oncology ,Surgery ,General Medicine - Abstract
The impact of sarcopenia on textbook outcome (TO) after hepatectomy in hepatocellular carcinoma (HCC) patients remains unclear. This study aimed to investigate the association between sarcopenia and TO, to clarify its long and short-term prognostic value, and to develop a nomogram model based on sarcopenia and TO for survival prediction.Patients who underwent HCC resection between January 2012 and March 2017 in three large hospitals in Fujian were retrospectively recruited and divided into sarcopenia and non-sarcopenia groups based on skeletal muscle index (SMI) values. TO was defined as no 30-day morality, no 30-day readmission, negative margins, no prolonged hospital stay, and no major complications. Multivariate regression was used to screen for clinical factors associated with TO. Nomograms of overall survival (OS) and recurrence-free survival (RFS) after hepatectomy for HCC were developed.A total of 1172 patients were included in the study. The TO rates were 28.74% (121/421 patients) in the sarcopenia group and 43.4% (326/751 patients) in the non-sarcopenia group. The results showed that sarcopenia was an independent predictor of TO (p 0.001), TO was an independent predictor of perioperative treatment-related sarcopenia (PTRS)(p = 0.002), and TO was an independent predictor of OS and RFS (p 0.001). Nomogram models based on sarcopenia and TO were generated and accurately predicted OS and RFS at 1, 3, and 5 years.Both sarcopenia and TO are independent predictors of OS and RFS after HCC resection. Sarcopenia was an independent predictor of TO. Sarcopenia influenced long-term survival by affecting short-term postoperative outcomes.
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- 2022
45. Adaptive Hierarchical Polling and Cost-Based Call Admission Control in IEEE 802.16 WiMAX Networks.
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Ben-Jye Chang, Yan-Ling Chen, and Chien-Ming Chou
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- 2007
- Full Text
- View/download PDF
46. Salvianolic Acid B Attenuates Iopromide-Induced Renal Tubular Epithelial Cell Injury by Inhibiting the TLR4/NF
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Ming Xin, Pei, Shu Jun, Dong, Xin Yue, Gao, Ting, Luo, Dong, Fan, Jun Feng, Jin, Xiao Duo, Zhao, and Yan Ling, Chen
- Abstract
Postcontrast acute kidney injury (PC-AKI) is directly caused by the use of contrast, indicating a clear causal relationship between the contrast and the injury. Salvianolic acid B (Sal B), a water-soluble compound of
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- 2022
47. Prognostic value of low microRNA-34a expression in human gastrointestinal cancer: a systematic review and meta-analysis
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Xiao-Lin Liu, Ling Li, and Yan-Ling Chen
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0301 basic medicine ,Oncology ,Cancer Research ,medicine.medical_specialty ,lcsh:RC254-282 ,03 medical and health sciences ,Gastrointestinal cancer ,0302 clinical medicine ,Surgical oncology ,Internal medicine ,microRNA-34a ,Genetics ,medicine ,Biomarkers, Tumor ,Humans ,Stage (cooking) ,Gastrointestinal Neoplasms ,business.industry ,Hazard ratio ,Odds ratio ,medicine.disease ,Prognosis ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Confidence interval ,Gene Expression Regulation, Neoplastic ,MicroRNAs ,Meta-analysis ,030104 developmental biology ,MicroRNA 34a ,030220 oncology & carcinogenesis ,business ,Research Article - Abstract
Background Mounting evidence shows that microRNA-34a (miR-34a) is involved in cancer prognosis. Therefore, we summarize the predictive role of miR-34a for survival in patients with gastrointestinal cancers (GICs). Methods All eligible studies were found by searching PubMed, Web of Science and EMBASE, and survival results were extracted. Then, the hazard ratio (HR) with the corresponding 95% confidence interval (CI) was calculated to evaluate the prognostic role of miR-34a in GICs. The association between miR-34a expression and clinicopathological characteristics was estimated by odds ratios (ORs) and 95% CIs. Results A total of 20 studies were included in this meta-analysis. For overall survival (OS), lower miR-34a expression could probably predict poorer outcome in GICs, with a pooled HR of 1.86 (95% CI: 1.52–2.28, P P Conclusion This meta-analysis revealed that lower miR-34a expression is significantly connected with worse OS and DFS/PFS/RFS in GIC patients. In addition, the miR-34a expression level is relatively lower in patients with lymph node metastasis than in patients without lymph node metastasis, and decreased miR-34a expression levels are linked to poor tumour differentiation and late TNM stage. MiR-34a may become a new factor for the prognosis prediction and progression of GICs.
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- 2021
48. Infestation of Oriental House Rat (Rattus tanezumi) with Chigger Mites Varies along Environmental Gradients across Five Provincial Regions of Southwest China
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Yan-Ling Chen, Xian-Guo Guo, Fan Ding, Yan Lv, Peng-Wu Yin, Wen-Yu Song, Cheng-Fu Zhao, Zhi-Wei Zhang, Rong Fan, Pei-Ying Peng, Bei Li, Ting Chen, and Dao-Chao Jin
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Health, Toxicology and Mutagenesis ,chigger mite ,rodent ,Public Health, Environmental and Occupational Health ,ectoparasite ,environmental heterogeneity ,Rattus tanezumi ,infestation - Abstract
Chigger mites are the exclusive vector of scrub typhus. Based on field investigations of 91 survey sites in 5 provincial regions of Southwest China, this paper reported variations of chigger infestation on the oriental house rat (Rattus tanezumi) along various environmental gradients. A total of 149 chigger species were identified from 2919 R. tanezumi in the 5 provincial regions, and Leptotrombidium deliense (a major vector of scrub typhus in China) was the first dominant chigger species, followed by Ascoschoengastia indica and Walchia ewingi. Rattus tanezumi had a stable overall prevalence (PM = 21.10%), mean abundance (MA = 7.01), and mean intensity (MI = 33.20) of chiggers with the same dominant mites in the whole Southwest China in comparison with a previous report in Yunnan Province, but chigger infestations on R. tanezumi varied along different environmental gradients. Rattus tanezumi in mountainous landscape had a higher infestation load of chiggers with higher species diversity than in flatland landscape. The infestation was higher at lower altitudes and latitudes. A high intensity of vegetation coverage was associated with high infestations. The results reflect the environmental heterogeneity of chiggers on the same host species. Warm climate and high relative humidity are beneficial to chigger infestation on R. tanezumi.
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- 2023
49. Maternal exposure to ambient levels of sulfur dioxide and risk of neural tube defects in 14 cities in Liaoning province, China: a population-based case–control study
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Jia-Yu Zhang, Jing Li, Yan-Hong Huang, Yan-Ling Chen, Shu Liu, Hui-Xu Dai, Cheng-Zhi Jiang, Zong-Jiao Chen, Qi-Jun Wu, and Li-Li Li
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China ,medicine.medical_specialty ,Epidemiology ,Population ,030501 epidemiology ,Toxicology ,Logistic regression ,03 medical and health sciences ,Air Pollution ,Humans ,Sulfur Dioxide ,Medicine ,Neural Tube Defects ,Cities ,education ,Air Pollutants ,education.field_of_study ,business.industry ,Confounding ,Public Health, Environmental and Occupational Health ,Case-control study ,Infant ,Odds ratio ,Pollution ,Confidence interval ,Quartile ,Maternal Exposure ,Case-Control Studies ,Female ,Particulate Matter ,0305 other medical science ,business ,Demography - Abstract
Epidemiological studies on the association of sulfur dioxide (SO2) with neural tube defects (NTDs) are lacking. The purpose of this study was to assess the aforementioned association through a population-based case–control study. This study involved 1457 NTDs cases and 7950 randomly selected healthy infants born in 14 cities in Liaoning province between 2010 and 2015. Ambient SO2 levels were acquired from 75 monitoring stations. The exposure assessment was based on the mean concentration of all stations in mother’s residential city. We used logistic regression models to assess the associations. In multivariable models adjusted for the confounding variables selected based on the 10 percent change-in-estimate method, we found that maternal SO2 exposure was positively associated with an increased risk of NTDs during the first month after conception (per 10 μg/m3 increase: adjusted odds ratio [aOR] = 1.02, 95% confidence interval [CI]: 1.00–1.04; highest versus lowest quartile: aOR = 2.55, 95% CI: 1.97–3.31) and the second month after conception (per 10 μg/m3 increase: aOR = 1.02, 95% CI: 1.00–1.04; highest versus lowest quartile: aOR=2.31, 95% CI: 1.77–3.00). For other exposure windows, positive associations also emerged in high- versus low-exposure analyses, except for the third month before conception; however, we could not further confirm significant findings from the continuous exposure analyses. Our study provides a new evidence that SO2 exposure may increase the risk of NTDs.
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- 2020
50. Chromosome-level genome assembly and resequencing of camphor tree (
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Xin-Dong, Wang, Chun-Yan, Xu, Yong-Jie, Zheng, Yan-Fang, Wu, Yue-Ting, Zhang, Ting, Zhang, Zhen-Yu, Xiong, Hai-Kuan, Yang, Jiang, Li, Chao, Fu, Feng-Ying, Qiu, Xiao-Ying, Dai, Xin-Liang, Liu, Xiao-San, He, Song-Song, Zhou, Sheng-Xing, Li, Tao, Fu, Han, Xie, Yan-Ling, Chen, Qian-Qian, Zhang, Hong-Qi, Wang, Yang-Dong, Wang, Cheng, Zhou, and Xiang-Mei, Jiang
- Published
- 2022
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