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In the process of oil and gas extraction, N80 steel is used as a common tubing material. CO2 corrosion has become one of the most dangerous problems in its entire life cycle. In this paper, the conditions of different temperatures (90-220 °C) and CO2 partial pressure (0.2-3 MPa) were selected, and the dynamic rotating high-temperature autoclave was used to simulate N80 steel corrosion in formation water environments. The results showed that the corrosion rate of N80 steel gradually decreased with the increase of temperature, and the corrosion rate was the lowest at 150 °C. In addition to this, with the increase of CO2 partial pressure, the corrosion rate first increased and then decreased. The corrosion rate was the highest when the CO2 partial pressure was 0.8 MPa. Through surface analysis techniques (SEM and XRD) and electrochemical tests, it was found that the corrosion resistance of N80 under high temperature and high pressure is closely related to the corrosion product film (FeCO3). The compactness of FeCO3 product film determines the corrosion characteristics of the matrix.

We aim to establish a simple and easy high-performance liquid chromatography system coupled with an ultraviolet detector suitable for simultaneous determination of 24 antiepileptic drugs in human plasma. Optimized chromatographic separation was performed on a ZORBAX Eclipse Plus-C18 (4.6 × 150 mm

Gut microbiota plays a key role in the pathophysiology of type 2 diabetes mellitus (T2D). Mulberry leaf has a hypoglycemic effect, but the potential mechanism is not fully understood. This study aimed to explore the influences and potential mechanisms of mulberry leaf water extract (MLWE) intervention on mice with T2D induced through a high-fat and high-sucrose diet combined with streptozotocin by the combination of fecal metabolomics and gut microbiota analysis. Results showed that MLWE could decrease fasting blood glucose and body weight while ameliorating lipid profiles, insulin resistance, liver inflammation, and the accumulation of lipid droplets in T2D mice. MLWE could reverse the abundances of the phyla Actinobacteria and Bacteroidetes and the ratio of Firmicutes/Bacteroidetes, and increase the abundances of the phyla Cyanobacteria and Epsilonbacteraeota in the feces of T2D mice. The abundances of genera Alloprevotella, Parabacteroides, Muribaculaceae, and Romboutsia in the feces of T2D mice could be reversed, while Oscillatoriales_cyanobacterium and Gastranaerophilales could be reinforced by MLWE supplementation. The levels of nine metabolites in the feces of T2D mice were improved, among which glycine, Phe-Pro, urocanic acid, phylloquinone, and lactate were correlated with Romboutsia and Gastranaerophilales. Taken together, we conclude that MLWE can effectively alleviate T2D by mediating the host-microbial metabolic axis.

A dual-template magnetic molecularly imprinted polymer (Dt-MMIP) with a specific recognition capability for carbamazepine (CBZ) and lamotrigine (LTG) was synthesized using methacrylic acid as a functional monomer, and ethylene glycol dimethylmethacrylate as a cross-linking agent. A magnetic non-molecularly imprinted polymer without templates (MNIP) was also prepared using the same procedure. The prepared polymers were characterized using scanning electron microscopy, Fourier-transform infrared spectroscopy and adsorption experiments. Results indicated that both Dt-MMIPs and MNIPs were microspherical nanoparticles, and the surface of the Dt-MMIP was rougher than that of the MNIP. In addition, the prepared Dt-MMIPs possessed a higher adsorption capacity and better selectivity for CBZ and LTG than the MNIPs. The maximum static adsorption capacities of Dt-MMIP for CBZ and LTG were 249.5 and 647.9 μg g

Mulberry leaves exhibit anti-lipogenic and lipid-lowering effects. However, the lipid biomarkers and underlying mechanisms for the improvement of the action of mulberry leaves on obesity and lipid metabolism disorders have not been sufficiently investigated yet. Herein, biochemical analysis combined with metabolomics targeting serum lipid mediators (oxylipins) were used to explore the efficacy and underlying mechanisms of mulberry leaf water extract (MLWE) in high-fat and high-sucrose diet (HFHSD)-fed mice. Our results showed that MLWE supplementation not only decreased body weight gain, serum total triglycerides, low-density lipoprotein cholesterol, alanine transaminase and aspartate transaminase levels, but also increased the serum level of high-density lipoprotein cholesterol. In addition, MLWE supplementation also ameliorated hepatic steatosis and lipid accumulation. These beneficial effects were associated with down-regulating genes involved in oxidative stress, inflammation, and lipogenesis such as acetyl-CoA carboxylase and fatty acid synthase, and up-regulating genes related to lipolysis that encoded peroxisome proliferator-activated receptor α, adiponectin (ADPN), adiponectin receptor (AdipoR) 1, AdipoR2, adenosine monophosphate-activated protein kinase (AMPK) and hormone-sensitive lipase. Moreover, a total of 54 serum lipid mediators were differentially changed in HFHSD-fed mice, among which 11 lipid mediators from n-3 polyunsaturated fatty acids (PUFAs) were apparently reversed by MLWE. These findings indicated that the ADPN/AMPK pathway, anti-inflammation, anti-oxidation, and n-3 PUFA metabolism played important roles in anti-obesity and improvement of lipid metabolism disorders modulated by MLWE supplementation.

With a pot experiment, the Biolog microplate and phospholipid fatty acid (PLFA) technology were used to explore whether the application of bacteria,将解淀粉芽孢杆菌(YB706)和伯克氏菌(BK8)外源添加至木麻黄盆栽苗中,运用Biolog生态板和磷脂脂肪酸(PLFA)技术,探究外施细菌能否改善连栽木麻黄土壤养分和微生物群落及植株生长。结果表明:与空白处理(CK)相比,YB706和BK8处理的木麻黄盆栽苗土壤碱解氮、速效磷显著增加,土壤全氮、全磷、全钾和速效钾无明显变化,幼苗株高分别增加59.1%和63.9%,BK8处理叶绿素含量提高81.9%。各处理平均颜色变化率(AWCD)呈现YB706CKBK8;除氨基酸类外,土壤微生物对不同碳源的利用率也表现为YB706CKBK8;经YB706和BK8处理的土壤微生物种类和数量均显著增加,除放线菌外,各类微生物PLFA总含量均为BK8YB706CK,与CK相比,土壤真菌/细菌有所提高。YB706和BK8处理的土壤微生物群落Simpson、Shannon、Brillouin和McIntosh指数均显著提高。表明外施YB706和BK8可促进木麻黄幼苗生长,且显著增加土壤速效养分含量,提高土壤微生物群落结构多样性,改善土壤微生物环境。.

Most people are dwelling in cities, which contain a high density of buildings constructed of concrete or similar materials. Conventional grey-colored concrete is absorptive to solar radiation and discharges the absorbed heat to the air as sensible heat, contributing to the development of urban heat island. While existing studies have attempted to find high-reflective concrete by varying the components of the concrete mix, the influence of cured conditions on the albedo of concrete samples is still unknown and has not been reported. Here we first prepared 120 cement pastes with different water-to-cement ratios and different cementitious materials, and then cured the pastes in an indoor environment and a moist chamber, respectively. After cured in a moist chamber for 28 days and then cured in the air for 365 days, the spectral reflectance of all the pastes was tested. Experimental results indicated that the pastes cured in a moist chamber have an albedo that is 0.01–0.10 greater than those of the pastes cured in an indoor environment, with a greater albedo difference at relatively higher water-to-cement ratios. The results also revealed that when concrete productions, such as concrete roof tiles and concrete paver blocks, are prepared, it is practical to cure such productions in a moist environment to get a high albedo.

Landslides distribute extensively in Rongxian county, the southeast of Guangxi province, China and pose great threats to this county. At present, hazard management strategy is facing an unprecedented challenge due to lack of a landslide susceptibility map. Therefore, the purpose of this paper was to construct a landslide susceptibility map by adopting three widely used models based on an integrated understanding of landslide’s characteristics. These models include a semi-quantitative method (SQM), information value model (IVM) and logistical regression model (LRM).The primary results show that (1) the county is classified into four susceptive regions, named as very low, low, moderate and high, which covered an area of 13.43%, 32.40%, 31.19% and 22.99% in SQM, 0.86%, 26.82%, 44.11%, and 28.21% in IVM, 9.88%, 17.73%, 46.36% and 26.03% in LRM; (2) landslides are likely to occur within the areas characterized by following obvious aspects: high intensity of human activities, slope angles of 25°~35°, the thickness of weathered soil is larger than 15 m; the lithology is granite, shale and mud rock; (3) the area under the curve of SQM, IVM and LRM is 0.7151, 0.7688 and 0.7362 respectively, and the corresponding success rate is 71.51%, 76.88% and 73.62%. It is concluded that these three models are acceptable because they have an effective capability of susceptibility assessment and can achieve an expected accuracy. In addition, the susceptibility outcome obtained from IVM provides a slightly higher quality than that from SQM, LRM.

Obesity is a worldwide problem that resulted from the excessive fat accumulation in adipose tissue, leading to the impairment of individual health. Mulberry leaf is an important traditional Chinese medicine and has been used to alleviate obesity for a long term. However, its underlying molecular mechanisms have not been fully elucidated yet.In this study, we aimed to investigate the inhibition effects of mulberry leaf water extract (MLWE) on lipid accumulation during the process of differentiation of 3T3-L1 preadipocytes and development of mature adipocytes through the combination of molecular biology assays and metabolomic analysis.The quality consistency and main chemical ingredients of MLWE were analyzed by high performance liquid chromatography and liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), respectively. Oil red O staining was used to mirror lipid accumulation. Lipogenesis-, lipolysis- and inflammation-related genes were evaluated by real-time PCR and western blot, respectively. Untargeted metabolomics were performed by LC-MS/MS.Prepared method and quality of MLWE were stable and reliable. A total of 34 compounds were identified and 14 of them were undoubtedly confirmed. MLWE supplementation could dose-dependently inhibit the aggregation of lipid droplets, and the expressions of sterol regulatory element-binding protein (SREBP)-1c, peroxisome proliferator-activated receptor (PPAR) γ, acetyl-CoA carboxylase (ACC), fatty acid synthase (FAS), tumor necrosis factor (TNF)-α and interleukin (IL)-6, and increase the expressions of adenosine monophosphate-activated protein kinase (AMPK), hormone-sensitive lipase (HSL) and IL-10 in the differentiation of preadipocytes. Furthermore, MLWE treatment could dose-dependently decrease the level of triglycerides and the expressions of ACC, FAS, TNF-α, and IL-6, and up-regulate the level of glycerol and the expressions of PPARα, adiponectin (ADPN), adiponectin receptor (AdipoR) 1, AdipoR2, AMPK, HSL, and IL-10 in the development of mature adipocytes. Untargeted metabolomics showed that a total of 5 and 18 differential metabolites were reversed by MLWE intervention in the differentiation of preadipocytes and the development of mature adipocytes, respectively, which involved in the biosynthesis of unsaturated fatty acids, arachidonic acid metabolism and glycerophospholipids metabolism.Taken together, this study firstly verified that MLWE could effectively alleviate lipid accumulation and inflammation by regulating ADPN/AMPK-mediated signaling pathways and relevant metabolic disturbances including biosynthesis of unsaturated fatty acids, arachidonic acid metabolism and glycerophospholipids metabolism.

To evaluate multidrug resistant loop-mediated isothermal amplification (MDR-LAMP) assay for the early diagnosis of multidrug-resistant tuberculosis and to compare the mutation patterns associated with theMDR-LAMP assay was evaluated using 100The sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of MDR-LAMP were 85.5%, 93.6%, 96.7%, and 74.4% for the detection of resistance to isoniazid and rifampicin, respectively, and 80.5%, 92.3%, 98.6%, and 41.4% for the detection ofMDR-LAMP is a rapid and accessible assay for the laboratory identification of rifampicin and isoniazid resistance of

The clastic rock slopes with mudstone interlayer are widely distributed in Guangxi, China. The mudstone interlayer is less permeable than the clastic rock, that may cause slope instability during the rainfall condition. In this study, a numerical analysis was conducted to study the effect of mudstone interlayer on clastic rock slope stability. A two-dimensional slope seepage model based on fluid–solid coupling was developed for the stability analysis of clastic rock slope with mudstone interlayer under different rainfall conditions. Rainfall-induced change of soil matrix suction in slope was analyzed by unstable seepage analysis and the effects of matrix suction on the slope stability and slip surface were then considered. Results indicated that the mudstone interlayer in clastic rock slope could develop to be the slip surface easily and made the clastic rock slope easy to fail under rainfall condition. When the soil matrix suction near the mudstone interlayer surface reaches a certain threshold value caused by rainfall intensity and duration, the most dangerous slip surface moves to the superficial layer, and the slope stability has a sudden drawdown.

Corrosion of steel reinforcement usually causes damage and deterioration of the reinforced concrete. Conventional corrosion experiments or analysis typically focus on the overall corrosion of the entire reinforcing steel bar while the distribution of the corrosion along the rebar has not yet been extensively investigated. In the present study the distribution of corrosive damage was investigated in a series of controlled, accelerated corrosion tests. The acoustic emission (AE) technique was employed to correlate the acoustic emission characteristics to the corrosion along the corroded rebar. A model for the corrosion damage distribution is established based on a Gaussian distribution function. The numerical modeling results match reasonably well with experimental results in almost all corrosion tests.

One dimensional Wiener degradation process is often used to describe the degradation of product performance. However one dimensional Wiener degradation process doesn’t sufficiently consider the relevance of multiple degradation factors and wear process, which lead to inaccurate results. To overcome these problems, a new two dimensional Wiener stochastic degradation model is proposed, which applys to the products on wear process and stochastic degradation process. Combining wear model and two dimensional Wiener stochastic degradation model, a new reliability analytical form is obtained by constructing the Fokker-Planck equation. Then using the relation among wear volume, degradation characteristic lifetime and drift parameter, parameters of two dimensional Wiener degradation model on the basic of wear model can be estimated. Compared with the existing approaches, the proposed method can effectively improve accuracy. Finally, a case study is illustrated the application and advantages of the proposed method.

We determined the genetic diversity of Mycobacterium tuberculosis (MTB) in a remote mountainous area of southwest China and evaluated the resolving ability of single nucleotide polymorphism (SNP) genotyping combined with variable number tandem repeat (VNTR) genotyping for Beijing family strains in association with drug resistance status.Three hundred thirty-one MTB strains were isolated from patients living in mountainous regions of southwest China, and 8-loci SNP, VNTR-15 genotyping assays, and drug susceptibility testing of 9 drugs were performed.A total of 183 [55.29% (183/331)] strains were classified into the Beijing family. Of the 183 strains, 111 (60.66%) were defined as modern Beijing strains. The most predominant modern Beijing sub-lineage and ancient Beijing sub-lineage were Bmyc10 [39.34% (72/183)] and Bmyc25 [20.77% (38/183)], respectively. Of the isolates, 19.64% (65/331) were resistant to at least 1 of the 9 anti-TB drugs and 17 [4.98% (17/331)] MTB isolates were multi-drug resistant tuberculosis (MDR-TB). Two hundred sixty-one isolates showed a clustering rate of 14.18% (37/261) and a discriminatory index of 0.9990. The Beijing lineage exhibited a significantly higher prevalence of MDR-TB, as well as resistance to isoniazid (INH), rifampin (RIF), and para-aminosalicylic acid (PAS) when analyzed independently (P = 0.005, P = 0.017, P = 0.014, and P = 0.006 respectively). The Beijing lineage was not associated with genetic clustering or resistance to any drug. In addition, genetic clustering was not associated with drug resistance.MTB strains demonstrate high genetic diversity in remote mountainous areas of southwest China. Beijing strains, especially modern Beijing strains, are predominant in remote mountainous area of China. The combination of 8-loci SNPs and VNTR-15 genotyping is a useful tool to study the molecular epidemiology of MTB strains in this area.

Mycobacterium avium (M. avium) and Mycobacterium intracellulare (M. intracellulare) are the major causative agents of nontuberculous mycobacteria (NTM)-related pulmonary infections. However, little is known about the differences in drug susceptibility profiles between these two species.A total of 393 NTM isolates were collected from Shanghai Pulmonary Disease Hospital. Sequencing of partial genes was performed to identify the strains at species level. The minimum inhibitory concentration (MIC) was used to evaluate the drug susceptibility against 20 antimicrobial agents. Variable number of tandem repeat (VNTR) typing was conducted to genotype these two species.A total of 173 (44.0%) M. avium complex (MAC) isolates were identified, including 41 (10.4%) M. avium isolates and 132 (33.6%) M. intracellulare isolates. Clarithromycin and amikacin were the two most effective agents against MAC isolates. The Hunter-Gaston Discriminatory Index (HGDI) values for VNTR typing of M. avium and M. intracellulare isolates were 0.993 and 0.995, respectively. Levofloxacin resistance was more common among the unclustered strains than among the clustered strains of M. intracellulare.M. intracellulare was the most common NTM species in China. Clarithromycin and amikacin had high antimicrobial activities against MAC. VNTR typing of MAC isolates revealed a high discriminatory power. Levofloxacin resistance was associated with unclustered strains of M. intracellulare.

China has a double burden of diabetes mellitus and tuberculosis, and many studies have been carried out on the mutual impact of these two diseases. This paper systematically reviewed studies conducted in China covering the mutual impact of epidemics of diabetes and tuberculosis, the impact of diabetes on multi-drug resistant tuberculosis and on the tuberculosis clinical manifestation and treatment outcome, the yields of bi-directional screening, and economic evaluation for tuberculosis screening among diabetes patients.

We performed molecular identification of clinical isolates of Mycobacterium fortuitum (M. fortuitum) and conducted drug susceptibility testing to analyze the in vitro susceptibility of clinical M. fortuitum isolates and potential molecular mechanism conferring resistance to fluoroquinolone and macrolide drugs. The results showed that moxifloxacin had the highest in vitro activity against M. fortuitum, and most M. fortuitum isolates were resistant to clarithromycin and linezolid in China. The loss of genetic mutation in clarithromycin- and amikacin-resistant isolates indicates that some other intrinsic mechanism conferring clarithromycin and amikacin resistance plays an essential role in M. fortuitum infection.

Objective To identify the proteins related to ofloxacin (OFX) resistance of Mycobacterium tuberculosis (MTB). Methods Standard MTB H37Rv strain, clinical isolates of OFX resistant strain (OFXR) and sensitive strain (OFXS) were obtained from the Chinese Center for Disease Control and Prevention, and they were cultured in Sauton's medium, and then inactivated by 60Co. Whole cellular proteins were extracted from OFXR, OFXS and H37Rv strain of MTB, respectively. The peptides were labeled, separated and identified by isobaric tags of relative and absolute quantitation (iTRAQ) combined with Nano LCMS/MS technology. Results One hundred and seventy-five and 134 differential expression proteins were identified in MTB OFXR compared with MTB OFXS and H37Rv, respectively. One hundred and four common differential expression proteins were identified in MTB OFXR compared with both MTB OFXS and H37Rv. The isoelectric point and theoretic relative molecular mass of differential expression proteins were widely distributed. The majority of the common differential expression proteins were involved in intermediary metabolism, respiration, and lipid metabolism. Twelve common differential expression proteins showed significant differences (the ratio>1.2 or

Numerical model of thermo-hydro-mechanical coupling processes of radioactive waste during the long-time disposal is established with the multi-fields coupling theory, based on the theory of continuum mechanics, thermo elastic material mechanics, and considered the influence of the variation of water viscosity due to temperature variation on permeability of pore water. The whole process of excavation,heating phase and cooling phase have been simulated in the calculation;and the calculated results are analyzed. It indicates that the anisotropy of thermo-hydro-mechanical behavior of the argillite rock is the main factor affecting temperature distribution,pore pressure and stress fields.The results make clear that the differences of coupling action and uncoupling action are obvious and the effects of coupling cannot be ignored.

For studying the mechanism of the composite soil nailing, this paper applies FLAC-3D to simulate pure soil nailing, soil nailing-soil anchor, soil nailing-micro pile-soil anchor. The analytical results as follows: (1)Because of the anchoring of the anchor, the axial tension of soil nailing in each row is much smaller than the one in the pure soil nailing supporting, and the tension of soil nailing which are closed to soil anchor decreases obviously. (2)The extrusion action of prestressed anchor makes the potential slip surface backward shifts and the sliding radius increases, which is better for the stability of foundation pit side (3)Micro-pile increases the strength of the soil in a certain range and improves the initial stress field before the excavation. (4)The inner force of the whole soil nailing and the prestress anchor declines but the drop is not large after the establishment of the micro-pile support in advance. (5)Prestressed anchor and micropile play a very important role in controlling the deformation of foundation pit.

For investigating the stiffness changes on reinforced concrete beam at different level of corrosion after fatigue, a group of RC beams were designed with different corrosion ratio, and fatigue test was carried on these specimens. The deflection, strain and crack of these specimens were analyzed in fatigue loading. The results indicate that the effect of corrosion ratio on fatigue performance of RC beams is very obviously.

To compare the performance of MTBDRplus V2 and Xpert MTB/RIF for detecting smear negative pulmonary tuberculosis (PTB).Clinical PTB suspects were enrolled consecutively in Anhui Chest Hospital and Xi'an Chest Hospital from January to December in 2014. The sputum samples of smear negative PTB suspects were collected and decontaminated. The sediment was used to conduct MTBDRplus V2, Xpert MTB/RIF and drug susceptibility test (DST). All the samples with discrepant drug susceptibility result between molecular methods and phenotypic method were confirmed by DNA sequencing.A total of 1973 cases were enrolled in this study. The detection rates of Mycobacterium tuberculosis complex (MTBC) by MTBDRplus V2 and Xpert MTB/RIF were 27.67% and 27.98%, respectively. When setting MGIT culture result as a gold standard, the sensitivity and specificity of MTBDRplus V2 were 86.74% and 93.84%, and the sensitivity and specificity of Xpert MTB/RIF were 86.55% and 93.43%, respectively. For the detection of the resistance to rifampin, the sensitivity and specificity of MTBDRplus V2 were 94.34% and 96.62%, and the sensitivity and specificity of Xpert MTB/RIF were 88.68% and 95.96%, respectively. For the detection of the resistance to isoniazid, the sensitivity and specificity of MTBDRplus V2 were 77.38% and 98.02%, respectively.MTBDRplus V2 and Xpert MTB/RIF can be used to detect MTBC in smear negative samples with satisfactory performance.

Effects of two sample preparation methods on tensile strength of red clay are studied. The first method adopts standard layered-bumping to make sample whose layer interfaces are vertical to tensile force. The second method uses compaction apparatus to prepare big sample and the big sample is cut to prepare triaxial sample whose layer interface is parallel to tensile force. Test results indicate that the layer interfaces of soil sample have large influence on the single axle tension test, because the results of the sample prepared by 1st method have large dispersion and low strength, while the results of the sample prepared by 2nd method are just the opposite.Moreover,the layer interfaces of soil sample have little influence on the unconfined compressive strength test, and the results of the tensile test and unconfined compressive strength test of the samples prepared by 2nd method meet with the Griffith Criterion, so the samples of uniaxial tension test should be prepared by 2nd method.

The shear strength of expansive soil has the obvious varying property with the changing of seasonal climatic. Based on Palmgrem-Miner linear accumulative damage theory, laboratory experiments on the strength of expansive soil combining with different wetting-drying cycle amplitudes were performed to discuss on the fatigue accumulative damage of expansive soil in the alternation of wetting-drying environment. The results show that：(1)The applying sequence of high (low) cycle amplitudes have significant effect upon the fatigue accumulative damage of expansive soil, and the strength attenuation of expansive soil is mainly controlled by the high circulate amplitude. (2) The Palmgrem-Miner linear accumulative damage criterion can basically reflect the fatigue accumulative damage process of expansive soil in the alternation wetting-drying environment, and the value of damage score “D” is close to 1 when the strength of expansive soil is stabled.

The wetting-drying effect of expansive soils plays an important role in slope stability analysis. According to the depth of atmospheric effect and the field observation data of moisture content, laboratory experiments on the strength of expansive soil with two wetting-drying cycle amplitudes were performed to discuss on the wetting-drying effect of expansive soil. The results show that the shear strength of expansive soil decreases with increasing number of cycles and finally reaches to a constant state. The value and cycle number of the constant state reduce with increase of amplitude of variation of water content. Furthermore, the stability analysis of expansive soil slope is studied with considering the wetting-drying effect. The results may provide the reference for the parameter selection in slope design.

The concrete which take coral fragments as aggregate can solve the shortage of building materials in the ocean reef island effectively, The coral concrete shall be classified as light weight aggregate concrete from the angle of the apparent density of building materials, its basic mechanical properties are similar to ordinary light weight aggregate, but there are also significantly different. The different between coral concrete and pumecrete、haydite concrete and the basic mechanics characteristics of coral concrete were study in this paper. The intensity、development of intensity with age、stress-strain relations、failure mode under load were discussed.