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Yuhan Qin, Yong Qiao, Dong Wang, Gaoliang Yan, Chengchun Tang, Genshan Ma Department of Cardiology, Zhongda Hospital, Medical School, Southeast University, Nanjing, Jiangsu, People’s Republic of ChinaCorrespondence: Gaoliang YanDepartment of Cardiology, Zhongda Hospital, Medical School, Southeast University, Nanjing, Jiangsu, People’s Republic of ChinaEmail yan_seu@hotmail.comObjective: Soluble urokinase-type plasminogen activator receptor (SuPAR) is a circulating protein and a novel identified promising biomarker for various renal diseases and kidney injury. However, it remains unknown on the predictive value of suPAR in contrast induced acute kidney injury (CI-AKI) in patients undergoing percutaneous coronary intervention (PCI).Methods: A total of 399 patients undergoing PCI were enrolled in the research from June 2020 to June 2021 in Zhongda Hospital. Patients were divided into CI-AKI and non-CI-AKI groups according to the preoperative and postoperative serum creatinine levels. Plasma suPAR level was detected through enzyme-linked immunosorbent assay on admission. Demographic data, hematological parameters, coronary angiography data and medications were recorded and compared between CI-AKI and non-CI-AKI groups. Logistic regression analysis and receiver operator characteristic (ROC) curve analysis were performed for identifying the independent risk factors of CI-AKI and assessment of the predictive value of suPAR for CI-AKI.Results: CI-AKI occurred in 65 (16.3%) patients undergoing PCI. The level of suPAR in CI-AKI group was significantly higher than that in the non-CI-AKI group. Multivariate logistic regression indicated diabetes, lower LVEF, lower hydration rate, lower baseline eGFR, higher plasma suPAR (OR = 2.875, 95% CI = 2.038– 3.672, P < 0.001) and volume of contrast media were all independent risk factors for CI-AKI after adjustment of the confounding factors. ROC analysis illustrated that the optimal area under the curve was 0.765, indicating pl

Gaoliang Yan, Renhua Sun, Zhongpu Chen, Xiaodong Pan, Zulong Sheng, Chengchun Tang Department of Cardiology, Zhongda Hospital of Southeast University Medical School, Nanjing, 210009, People’s Republic of ChinaCorrespondence: Gaoliang YanDepartment of Cardiology, Zhongda Hospital of Southeast University Medical School, Nanjing, 210009, People’s Republic of ChinaTel +86 18761890380Email yangaol@hotmail.comPurpose: Pulmonary hypertension (PH) is a pathological process mainly characterized by the progressive increase in pulmonary vascular resistance. The degradation of pulmonary artery smooth muscle cells (PASMCs) from contractile/differentiated phenotype to synthetic/dedifferentiated phenotype is a key factor for hypoxic pulmonary hypertension.Materials and Methods: In this study, qPCR was performed to evaluate the gene expression of mRNAs. Western blot, immunofluorescence and RNA pull down were used to detect gene expression levels.Results: We found that the gene expression of polypyrimidine tract-binding protein1 (PTBP1) was increased significantly in a time-dependent manner in rats PA tissues and PASMCs after hypoxia. PTBP1 knockdown can inhibit the phenotypic transition of PASMCs. PTBP1 inhibits the phenotypic transition of PASMCs. In addition, PTBP1 inhibits the integrin-linked kinase (ILK) expression under hypoxic conditions, thereby down-regulating the expression of downstream proteins. It inhibits the phenotypic transition of PASMCs and alleviates pulmonary hypertension.Conclusion: In conclusion, PTBP1/ILK axis promotes the development of PH via inducing phenotypic transition of PASMCs. This may provide a novel therapy for PH.Keywords: pulmonary hypertension, polypyrimidine tract-binding protein 1, integrin-linked kinase, pulmonary artery smooth muscle cells

Gaoliang Yan, Chengchun Tang, Genshan Ma Department of Cardiology, Zhongda Hospital of Southeast University Medical School, Nanjing, 210009, People’s Republic of ChinaCorrespondence: Gaoliang Yan; Genshan MaDepartment of Cardiology, Zhongda Hospital of Southeast University Medical School, No. 87 Dingjiaqiao, Gulou District, Nanjing, 210009, People’s Republic of ChinaTel/Fax +86 25 83262413; +86 25 83262410Email yan_seu@hotmail.com; magenshan@hotmail.comBackground: Higher serum myeloperoxidase (MPO) in patients with acute coronary syndrome is associated with adverse cardiovascular outcomes. Contrast-induced nephropathy (CIN) is associated with worse prognosis in patients with coronary artery disease following angiography. We have no idea whether patients with higher serum myeloperoxidase have a higher risk of CIN in acute myocardial infarction (AMI) after percutaneous coronary intervention (PCI).Methods: This study involved 436 consecutive patients with AMI who had received PCI. Serum MPO levels were determined using enzyme-linked immunosorbent assay before administration of contrast media. Multivariate logistic regression analysis was used to analyze the independent risk factors for CIN after univariate analysis. The receiver operator characteristic (ROC) analysis was performed to evaluate the predictive value of MPO for CIN.Results: Among the 436 patients, 79 individuals (18.1%) suffered CIN after the PCI procedure. Patients who developed CIN had significantly higher MPO levels compared to those who did not ([203.8 (150.6– 276.2)] versus [138.5 (129.9– 149.2)]; p< 0.001). Multivariate logistic regression analysis revealed that MPO level (OR 1.023, 95% CI: 1.017– 1.029, p< 0.001) was an independent risk factor for the incidence of CIN after adjusting for the baseline information, blood indicators and angiography procedural parameters. The area under the ROC curve for predicting CIN of MPO was 0.848, and

Gaoliang Yan, Dong Wang, Chengchun Tang, Genshan Ma Department of Cardiology, Zhongda Hospital of Southeast University Medical School, Nanjing, Jiangsu, People’s Republic of ChinaCorrespondence: Genshan MaDepartment of Cardiology, Zhongda Hospital of Southeast University Medical School, No. 87 Dingjiaqiao, Gulou District, Nanjing, Jiangsu, 210009, People’s Republic of ChinaTel/Fax +86 25 83262410Email Dr_magenshan@hotmail.comObjective: The association of lactate and contrast-induced acute kidney injury (CI-AKI) has not been well established. This prospective study was planned to identify the effects of lactate level on the occurrence of CI-AKI and long-term prognosis with acute myocardial infarction (AMI) patients undergoing emergency percutaneous coronary intervention (PCI).Methods: A total of 280 patients with AMI who underwent emergency PCI were selected from March 2018 to March 2019. A receiver operating characteristic (ROC) curve was used to analyze the optimal cut-off value of lactate on predicting CI-AKI after PCI. A multivariable logistic regression model was used to explore the significant predictors that might affect the occurrence of CI-AKI after univariate analysis. The primary endpoints were clinical outcomes including events: a combined endpoint of major adverse cardiovascular events, re-hospitalization due to heart failure, and worsening renal function. The Cox regression model was further used to analyze the predictors of the long-term prognosis after PCI.Results: Among the 280 patients, 64 patients (22.9%) developed CI-AKI after emergency PCI procedure. Multivariable logistic regression analysis revealed that baseline lactate level was the independent risk factor for the development of CI-AKI (OR, 3.657; 95% CI, 2.237– 5.978; p< 0.001). The area under the ROC curve for predicting CI-AKI of lactate was 0.786, and the optimum cut-off point of lactate was 3.02 mmol/L, with sensitivity of 65.6% and specificity of 85.2%. The incidence of primary

Xianjing Wei,1 Ying Zhang,1 Gaoliang Yan,2 Xiaoqing Wang1 1Department of Cardiology, The Affiliated Zhongshan Hospital of Dalian University, Dalian, Liaoning, 110016, People’s Republic of China; 2Department of Cardiology, Zhongda Hospital of Southeast University Medical School, Nanjing, Jiangsu, People’s Republic of ChinaCorrespondence: Xianjing WeiDepartment of Cardiology, The Affiliated Zhongshan Hospital of Dalian University, No. 6 Jiefang Street, Zhongshan District, Dalian City, Liaoning Province, 110016, People’s Republic of ChinaTel +86 18642668608Email weixian_jing@163.comObjective: To investigate the relationship between chronic kidney disease (CKD) and the severity and long-term prognosis of patients with coronary artery disease (CAD) after drug-eluting stent (DES) implantation.Methods: There were 814 patients, who consecutively received a DES implantation, selected for this study. They were divided into two groups, according to whether or not they suffered CKD. There were 254 cases in the CKD group (31.2%), while there were 560 cases (68.8%) in the control group. The clinical characteristics, coronary artery lesions, and major adverse cardiac and cerebrovascular events (MACCE) of the two groups were compared, and the relationship between risk factors and MACCEs was analyzed by multivariate logistic regression.Results: Compared with the control group, the CKD group had more severe coronary artery stenosis, expressed as the more diseased arteries (2.15 ± 0.82 vs 1.87 ± 0.83, p = 0.001), a high incidence of three diseased arteries (42.0% vs 28.3%, p = 0.001), and a higher Gensini score [37 (18.6, 66) vs 27.5 (12, 52.5), p = 0.009]. The one-year post-implant incidence of MACCE was higher in the CKD group compared with the control group (17.6% vs 9.9%, p = 0.006).Conclusion: CKD appears to be an important predictor for the prognosis of CAD.Keywords: chronic kidney disease, coronary artery disease, Gensini scor