Search

Your search keyword '"Yamashiro, Chiemi"' showing total 22 results
Start Over Author "Yamashiro, Chiemi"
22 results on '"Yamashiro, Chiemi"'

4. Early manifestations and differential gene expression associated with photoreceptor degeneration in Prom1-deficient retina

Author

Kobayashi, Yuka, Watanabe, Shizuka, Ong, Agnes Lee Chen, Shirai, Manabu, Yamashiro, Chiemi, Ogata, Tadahiko, Higashijima, Fumiaki, Yoshimoto, Takuya, Hayano, Takahide, Asai, Yoshiyuki, Sasai, Noriaki, Kimura, Kazuhiro, Kobayashi, Yuka, Watanabe, Shizuka, Ong, Agnes Lee Chen, Shirai, Manabu, Yamashiro, Chiemi, Ogata, Tadahiko, Higashijima, Fumiaki, Yoshimoto, Takuya, Hayano, Takahide, Asai, Yoshiyuki, Sasai, Noriaki, and Kimura, Kazuhiro

Abstract

Retinitis pigmentosa (RP) and macular dystrophy (MD) are characterized by gradual photoreceptor death in the retina and are often associated with genetic mutations, including those in the prominin-1 (Prom1) gene. Prom1-knockout (KO) mice recapitulate key features of these diseases including light-dependent retinal degeneration and constriction of retinal blood vessels. The mechanisms underlying such degeneration have remained unclear, however. We here analysed early events associated with retinal degeneration in Prom1-KO mice. We found that photoreceptor cell death and glial cell activation occur between 2 and 3 weeks after birth. Whereas gene expression was not affected at 2 weeks, the expression of several genes was altered at 3 weeks in the Prom1-KO retina, with the expression of that for endothelin-2 (Edn2) being markedly upregulated. Expression of Edn2 was also induced by light stimulation in Prom1-KO mice reared in the dark. Treatment with endothelin receptor antagonists attenuated photoreceptor cell death, gliosis and retinal vessel stenosis in Prom1-KO mice. Our findings thus reveal early manifestations of retinal degeneration in a model of RP/MD and suggest potential therapeutic agents for these diseases.This article has an associated First Person interview with the first author of the paper.

Published
2023

5. Role of CRP2-MRTF interaction in functions of myofibroblasts

Author

Hayashi, Ken’ichiro, primary, Horoiwa, Shinri, additional, Mori, Kotaro, additional, Miyata, Hiroshi, additional, Labios, Reuben Jacob, additional, Morita, Tsuyoshi, additional, Kobayashi, Yuka, additional, Yamashiro, Chiemi, additional, Higashijima, Fumiaki, additional, Yoshimoto, Takuya, additional, Kimura, Kazuhiro, additional, and Nakagawa, Yoshiaki, additional

Published
2023
Full Text
View/download PDF

6. Early manifestations and differential gene expression associated with photoreceptor degeneration in Prom1-deficient retina

Author

Kobayashi, Yuka, primary, Watanabe, Shizuka, additional, Ong, Agnes Lee Chen, additional, Shirai, Manabu, additional, Yamashiro, Chiemi, additional, Ogata, Tadahiko, additional, Higashijima, Fumiaki, additional, Yoshimoto, Takuya, additional, Hayano, Takahide, additional, Asai, Yoshiyuki, additional, Sasai, Noriaki, additional, and Kimura, Kazuhiro, additional

Published
2021
Full Text
View/download PDF

8. Study protocol for a multicentre, open-label, single-arm phase I/II trial to evaluate the safety and efficacy of ripasudil 0.4% eye drops for retinopathy of prematurity

Author

Arima, Mitsuru, primary, Inoue, Hirosuke, additional, Nakao, Shintaro, additional, Misumi, Akiko, additional, Suzuki, Maya, additional, Matsushita, Itsuka, additional, Araki, Shunsuke, additional, Yamashiro, Chiemi, additional, Takahashi, Kazumasa, additional, Ochiai, Masayuki, additional, Yoshida, Noriko, additional, Hirose, Masayuki, additional, Kishimoto, Junji, additional, Todaka, Koji, additional, Hasegawa, Shunji, additional, Kimura, Kazuhiro, additional, Kusuhara, Koichi, additional, Kondo, Hiroyuki, additional, Ohga, Shouichi, additional, and Sonoda, Koh-Hei, additional

Published
2021
Full Text
View/download PDF

9. Early manifestations and differential gene expression associated with photoreceptor degeneration in Prom1-deficient retina

Author

Kobayashi, Yuka, Watanabe, Shizuka, Ong, Agnes Lee Chen, Shirai, Manabu, Yamashiro, Chiemi, Ogata, Tadahiko, Higashijima, Fumiaki, Yoshimoto, Takuya, Hayano, Takahide, Asai, Yoshiyuki, Sasai, Noriaki, Kimura, Kazuhiro, Kobayashi, Yuka, Watanabe, Shizuka, Ong, Agnes Lee Chen, Shirai, Manabu, Yamashiro, Chiemi, Ogata, Tadahiko, Higashijima, Fumiaki, Yoshimoto, Takuya, Hayano, Takahide, Asai, Yoshiyuki, Sasai, Noriaki, and Kimura, Kazuhiro

Abstract

Retinitis pigmentosa (RP) and macular dystrophy (MD) are characterized by gradual photoreceptor death in the retina and are often associated with genetic mutations, including those in the prominin-1 (Prom1) gene. Prom1-knockout (KO) mice recapitulate key features of these diseases including light-dependent retinal degeneration and constriction of retinal blood vessels. The mechanisms underlying such degeneration have remained unclear, however. We here analysed early events associated with retinal degeneration in Prom1-KO mice. We found that photoreceptor cell death and glial cell activation occur between 2 and 3 weeks after birth. Whereas gene expression was not affected at 2 weeks, the expression of several genes was altered at 3 weeks in the Prom1-KO retina, with the expression of that for endothelin-2 (Edn2) being markedly upregulated. Expression of Edn2 was also induced by light stimulation in Prom1-KO mice reared in the dark. Treatment with endothelin receptor antagonists attenuated photoreceptor cell death, gliosis and retinal vessel stenosis in Prom1-KO mice. Our findings thus reveal early manifestations of retinal degeneration in a model of RP/MD and suggest potential therapeutic agents for these diseases. This article has an associated First Person interview with the first author of the paper., identifier:1754-8403

Published
2021

10. Light-dependent induction of Edn2 expression and attenuation of retinal pathology by endothelin receptor antagonists in Prominin-1- deficient mice

Author

Kobayashi Yuka, Higashijima Fumiaki, Sasai Noriaki, Watanabe Shizuka, Shirai Manabu, Kimura Kazuhiro, Ogata Tadahiko, Hayano Takahide, Yoshimoto Takuya, Asai Yoshiyuki, and Yamashiro Chiemi

Subjects
Retinal degeneration, Pathology, medicine.medical_specialty, Retina, business.industry, Retinal, medicine.disease, Photoreceptor cell, chemistry.chemical_compound, medicine.anatomical_structure, chemistry, Gliosis, Retinitis pigmentosa, medicine, medicine.symptom, Endothelin receptor, business, Cell activation
Abstract

Retinitis pigmentosa (RP) and macular dystrophy (MD) are prevalent retinal degenerative diseases associated with gradual photoreceptor death. These diseases are often caused by genetic mutations that result in degeneration of the retina postnatally after it has fully developed. The Prominin-1 gene (Prom1) is a causative gene for RP and MD, and Prom1- knockout (KO) mice recapitulate key features of these diseases including light-dependent retinal degeneration and stenosis of retinal blood vessels. The mechanisms underlying progression of such degeneration have remained unknown, however. We here analysed early events associated with retinal degeneration in Prom1-KO mice. We found that photoreceptor cell death and glial cell activation occur between 2 and 3 weeks after birth. High-throughput analysis revealed that expression of the endothelin-2 gene (Edn2) was markedly up-regulated in the Prom1-deficient retina during this period. Expression of Edn2 was also induced by light stimulation in Prom1-KO mice that had been reared in the dark. Finally, treatment with endothelin receptor antagonists attenuated photoreceptor cell death, gliosis, and retinal vessel stenosis in Prom1-KO mice. Our findings suggest that inhibitors of endothelin signalling may delay the progression of RP and MD and therefore warrant further study as potential therapeutic agents for these diseases.

Published
2020
Full Text
View/download PDF

11. Light-dependent induction of Edn2 expression and attenuation of retinal pathology by endothelin receptor antagonists in Prominin-1- deficient mice

Author

Kobayashi, Yuka, primary, Watanabe, Shizuka, additional, Shirai, Manabu, additional, Yamashiro, Chiemi, additional, Ogata, Tadahiko, additional, Higashijima, Fumiaki, additional, Yoshimoto, Takuya, additional, Hayano, Takahide, additional, Asai, Yoshiyuki, additional, Sasai, Noriaki, additional, and Kimura, Kazuhiro, additional

Published
2020
Full Text
View/download PDF

12. Influence of extended depth of focus intraocular lenses on visual field sensitivity

Author

Takahashi, Makiko, primary, Yamashiro, Chiemi, additional, Yoshimoto, Takuya, additional, Kobayashi, Yuka, additional, Higashijima, Fumiaki, additional, Kobayashi, Masaaki, additional, Hatano, Makoto, additional, Ohta, Manami, additional, Nagai, Tomohiko, additional, Teranishi, Shinichiro, additional, Suzuki, Katsuyoshi, additional, Takabatake, Ryu, additional, and Kimura, Kazuhiro, additional

Published
2020
Full Text
View/download PDF

13. Correction: Dendritic cells mediate the anti-inflammatory action of omega-3 long-chain polyunsaturated fatty acids in experimental autoimmune uveitis

Author

Uchi, Sho-Hei, primary, Yanai, Ryoji, additional, Kobayashi, Masaaki, additional, Hatano, Makoto, additional, Kobayashi, Yuka, additional, Yamashiro, Chiemi, additional, Nagai, Tomohiko, additional, Tokuda, Kazuhiro, additional, Connor, Kip M., additional, Sonoda, Koh-Hei, additional, and Kimura, Kazuhiro, additional

Published
2020
Full Text
View/download PDF

15. Quantitative Evaluation of Vitreous Opacities Using Motion Video Through Slit-Lamp Examination.

Author

Furukawa, Shota, Matsui, Wakana, Suetake, Noriaki, Uchino, Eiji, Yamashiro, Chiemi, Takabatake, Ryu, Takahashi, Makiko, and Kimura, Kazuhiro

Subjects
EYE, INTRAOCULAR lenses, TURBIDITY, SLIT lamp microscopy, LENGTH measurement
Abstract

This study proposed a quantitative evaluation method for vitreous opacities using motion video. The proposed method focused on moving turbidity in the vitreous. The moving turbidity appeared as an inter-frame difference, which was calculated from two consecutive frames. Therefore, the degree of vitreous opacity was estimated using this inter-frame difference. The proposed method was applied in the experiments to actual motion videos obtained using slit-lamp examination. The effectiveness of method was confirmed using the t-test and linear discriminant method. [ABSTRACT FROM AUTHOR]

Published
2022
Full Text
View/download PDF

18. Dendritic cells mediate the anti-inflammatory action of omega-3 long-chain polyunsaturated fatty acids in experimental autoimmune uveitis

Author

Uchi, Sho-Hei, primary, Yanai, Ryoji, additional, Kobayashi, Masaaki, additional, Hatano, Makoto, additional, Kobayashi, Yuka, additional, Yamashiro, Chiemi, additional, Nagai, Tomohiko, additional, Tokuda, Kazuo, additional, Connor, Kip M., additional, Sonoda, Koh-Hei, additional, and Kimura, Kazuhiro, additional

Published
2019
Full Text
View/download PDF

20. Up‐regulation of the pentose phosphate pathway and HIF‐1α expression during neural progenitor cell induction following glutamate treatment in rat ex vivo retina.

Author

Tokuda, Kazuhiro, Baron, Byron, Yamashiro, Chiemi, Kuramitsu, Yasuhiro, Kitagawa, Takao, Kobayashi, Masaaki, Sonoda, Koh‐Hei, and Kimura, Kazuhiro

Subjects
PENTOSE phosphate pathway, NICOTINAMIDE adenine dinucleotide phosphate, HYPOXIA-inducible factor 1, RETINA, GLUCOSE-6-phosphate dehydrogenase, DNA synthesis, PYRUVATE kinase, PROGENITOR cells
Abstract

The metabolic state influences the regulation of neural stem/progenitor cells. The pentose phosphate pathway (PPP), an alternative metabolic pathway that operates parallel to glycolysis, not only provides key intermediates for biosynthetic reactions but also controls the fate of neural stem/progenitor cells. We have previously shown that glutamate application leads to the induction of neural progenitor cells in mature ex vivo rat retina. In this study, we investigated whether regulation of the PPP might be changed following glutamate treatment of the retina. Immunoblot analysis revealed that the amount of glucose‐6‐phosphate dehydrogenase (G6PD), the rate‐limiting enzyme of the PPP as well as that of 6‐phosphogluconate dehydrogenase (6PGD), another enzyme in this pathway, increased in the glutamate‐treated retina. Consistent with the fact that both these enzymes generate reduced nicotinamide adenine dinucleotide phosphate (NADPH), the amount of NAPDH in the treated retina was significantly higher compared with that in the untreated retina. We also found that both DNA synthesis as well as the expression of fatty acid synthase (FASN) increased significantly in the glutamate‐treated retina. Furthermore, hypoxia‐inducible factor 1‐α (HIF‐1α), a positive transcriptional regulator of PPP enzymes, was up‐regulated at both messenger RNA (mRNA) and protein levels. Finally, we found the interaction of HIF‐1α with the M2 isozyme of pyruvate kinase (PKM2), with this interaction having been shown to contribute to a positive feedback loop in the control of glycolysis. Our results thus show that specific metabolic change in the PPP occurs in the process of neural progenitor cell induction in the mature rat retina. [ABSTRACT FROM AUTHOR]

Published
2020
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results