118 results on '"Yamaga, K."'
Search Results
2. Cytokine mRNA repertoire of articular chondrocytes from arthritic patients, infants, and neonatal mice
- Author
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Tanabe, B. K., Abe, L. M., Kimura, L. H., Reinker, K. A., and Yamaga, K. M.
- Published
- 1996
- Full Text
- View/download PDF
3. 1319 Claudin-1 and -3 maintain proper hair follicle structure and regulate telogen effluvium
- Author
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Yamaga, K., primary, Tokumasu, R., additional, Yamamoto, A., additional, Tamura, A., additional, Katayama, I., additional, Murota, H., additional, and Tsukita, S., additional
- Published
- 2018
- Full Text
- View/download PDF
4. Isolation and Partial Characterization of the Major Histocompati-Bility Antigen in the Chicken
- Author
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Kubo, R. T., Yamaga, K., Abplanalp, H. A., and Benedict, Albert A., editor
- Published
- 1977
- Full Text
- View/download PDF
5. Report from the fourth international consensus meeting to harmonize core outcome measures for atopic eczema/dermatitis clinical trials (HOME initiative): British Journal of Dermatology
- Author
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Chalmers, J. R., Simpson, E., Apfelbacher, C. J., Thomas, S.K., von Kobyletzki, L., Schmitt, J., Singh, J.A., Svensson, A., Williams, H.C., Abuabara, K., Aoki, V., Ardeleanu, M., Awici-Rasmussen, M., Barbarot, S., Berents, T. L., Block, J., Bragg, A., Burton, T., Clemmensen, K. K. B., Creswell-Melville, A., Dinesen, M., Drucker, A., Eckert, R.L., Flohr, C., Garg, M., Gerbens, L. A. A., Graff, A. L. B., Hanifin, J., Heinl, D., Humphreys, R., Ishii, H.A., Kataoka, Y., Leshem, Y. A., Marquort, B., Massuel, M. A., Merhand, S., Mizutani, H., Murota, H., Murrell, D.F., Nakahara, T., Nasr, I., Nograles, K., Ohya, Y., Osterloh, I., Pander, J., Prinsen, C., Purkins, L., Ridd, M.J., Sach, T., Schuttelaar, M. L. A., Shindo, S., Smirnova, J., Sulzer, A., Gjerde, E. S., Takaoka, R., Talmo, H. V., Tauber, M., Torchet, F., Volke, A., Wahlgren, C.F., Weidinger, S., Weisshaar, E., Wollenberg, A., Yamaga, K., Zhao, C. Y., Spuls, P.I., Epidemiology and Data Science, and EMGO - Quality of care
- Abstract
This article is a report of the fourth meeting of the Harmonising Outcome Measures for Eczema (HOME) initiative held in Malmo, Sweden on 23-24 April 2015 (HOME IV). The aim of the meeting was to achieve consensus over the preferred outcome instruments for measuring patient-reported symptoms and quality of life for the HOME core outcome set for atopic eczema (AE). Following presentations, which included data from systematic reviews, consensus discussions were held in a mixture of whole group and small group discussions. Small groups were allocated a priori to ensure representation of different stakeholders and countries. Decisions were voted on using electronic keypads. For the patient-reported symptoms, the group agreed by vote that itch, sleep loss, dryness, redness/inflamed skin and irritated skin were all considered essential aspects of AE symptoms. Many instruments for capturing patient-reported symptoms were discussed [ including the Patient-Oriented SCOring Atopic Dermatitis index, Patient-Oriented Eczema Measure (POEM), Self-Administered Eczema Area and Severity Index, Itch Severity Scale, Atopic Dermatitis Quickscore and the Nottingham Eczema Severity Score] and, by consensus, POEM was selected as the preferred instrument to measure patient-reported symptoms. Further work is needed to determine the reliability and measurement error of POEM. Further work is also required to establish the importance of pain/soreness and the importance of collecting information regarding the intensity of symptoms in addition to their frequency. Much of the discussion on quality of life concerned the Dermatology Life Quality Index and Quality of Life Index for Atopic Dermatitis; however, consensus on a preferred instrument for measuring this domain could not be reached. In summary, POEM is recommended as the HOME core outcome instrument for measuring AE symptoms.
- Published
- 2016
6. Report from the fourth international consensus meeting to harmonize core outcome measures for atopic eczema/dermatitis clinical trials (HOME initiative)
- Author
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Chalmers, J. R., Simpson, Elizabeth M, Apfelbacher, C. J., Thomas, K. S., von Kobyletzki, L., Schmitt, J., Singh, Jasvinder A, Svensson, Staffan, Williams, H. C., Abuabara, K., Aoki, V., Ardeleanu, M., Awici-Rasmussen, M., Barbarot, S., Berents, T. L., Block, J, Bragg, A., Burton, T., Clemmensen, K. K. Bjerring, Creswell-Melville, A., Dinesen, M., Drucker, A., Eckert, L., Flohr, Carsten, Garg, M., Gerbens, L. A A, Graff, A. L B, Hanifin, Jon M, Heinl, D., Humphreys, R. D., Ishii, H. A., Kataoka, Y., Leshem, Y. A., Marquort, B., Massuel, M. A., Merhand, S., Mizutani, H., Murota, H., Murrell, D. F., Nakahara, T., Nasr, I., Nograles, K., Ohya, Y., Osterloh, I., Pander, J., Prinsen, C., Purkins, L., Ridd, M., Sach, T., Schuttelaar, Marie-Louise A, Shindo, S., Smirnova, J., Sulzer, A., Synnøve Gjerde, E., Takaoka, R., Vestby Talmo, H., Tauber, M, Torchet, F., Volke, A., Wahlgren, C. F., Weidinger, S, Weisshaar, Elke, Wollenberg, A., Yamaga, K., Zhao, Z. Y., Spuls, P. I., Chalmers, J. R., Simpson, Elizabeth M, Apfelbacher, C. J., Thomas, K. S., von Kobyletzki, L., Schmitt, J., Singh, Jasvinder A, Svensson, Staffan, Williams, H. C., Abuabara, K., Aoki, V., Ardeleanu, M., Awici-Rasmussen, M., Barbarot, S., Berents, T. L., Block, J, Bragg, A., Burton, T., Clemmensen, K. K. Bjerring, Creswell-Melville, A., Dinesen, M., Drucker, A., Eckert, L., Flohr, Carsten, Garg, M., Gerbens, L. A A, Graff, A. L B, Hanifin, Jon M, Heinl, D., Humphreys, R. D., Ishii, H. A., Kataoka, Y., Leshem, Y. A., Marquort, B., Massuel, M. A., Merhand, S., Mizutani, H., Murota, H., Murrell, D. F., Nakahara, T., Nasr, I., Nograles, K., Ohya, Y., Osterloh, I., Pander, J., Prinsen, C., Purkins, L., Ridd, M., Sach, T., Schuttelaar, Marie-Louise A, Shindo, S., Smirnova, J., Sulzer, A., Synnøve Gjerde, E., Takaoka, R., Vestby Talmo, H., Tauber, M, Torchet, F., Volke, A., Wahlgren, C. F., Weidinger, S, Weisshaar, Elke, Wollenberg, A., Yamaga, K., Zhao, Z. Y., and Spuls, P. I.
- Abstract
This article is a report of the fourth meeting of the Harmonising Outcome Measures for Eczema (HOME) initiative held in Malmö, Sweden on 23–24 April 2015 (HOME IV). The aim of the meeting was to achieve consensus over the preferred outcome instruments for measuring patient-reported symptoms and quality of life for the HOME core outcome set for atopic eczema (AE). Following presentations, which included data from systematic reviews, consensus discussions were held in a mixture of whole group and small group discussions. Small groups were allocated a priori to ensure representation of different stakeholders and countries. Decisions were voted on using electronic keypads. For the patient-reported symptoms, the group agreed by vote that itch, sleep loss, dryness, redness/inflamed skin and irritated skin were all considered essential aspects of AE symptoms. Many instruments for capturing patient-reported symptoms were discussed [including the Patient-Oriented SCOring Atopic Dermatitis index, Patient-Oriented Eczema Measure (POEM), Self-Administered Eczema Area and Severity Index, Itch Severity Scale, Atopic Dermatitis Quickscore and the Nottingham Eczema Severity Score] and, by consensus, POEM was selected as the preferred instrument to measure patient-reported symptoms. Further work is needed to determine the reliability and measurement error of POEM. Further work is also required to establish the importance of pain/soreness and the importance of collecting information regarding the intensity of symptoms in addition to their frequency. Much of the discussion on quality of life concerned the Dermatology Life Quality Index and Quality of Life Index for Atopic Dermatitis; however, consensus on a preferred instrument for measuring this domain could not be reached. In summary, POEM is recommended as the HOME core outcome instrument for measuring AE symptoms.
- Published
- 2016
7. 678 Claudin-3 is expressed in sweat glands and prevents the leakage of sweat
- Author
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Yamaga, K., primary, Murota, H., additional, Tamura, A., additional, Tsukita, S., additional, and Katayama, I., additional
- Published
- 2016
- Full Text
- View/download PDF
8. Group A Streptococcal Isolates Temporally Associated with Acute Rheumatic Fever in Hawaii: Differences from the Continental United States
- Author
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Erdem, G., primary, Mizumoto, C., additional, Esaki, D., additional, Reddy, V., additional, Kurahara, D., additional, Yamaga, K., additional, Abe, L., additional, Johnson, D., additional, Yamamoto, K., additional, and Kaplan, E. L., additional
- Published
- 2007
- Full Text
- View/download PDF
9. Molecular Epidemiologic Comparison of 2 Unusual Clusters of Group A Streptococcal Necrotizing Fasciitis in Hawaii
- Author
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Erdem, G., primary, Ford, J. M., additional, Kanenaka, R. Y., additional, Abe, L., additional, Yamaga, K., additional, and Effler, P. V., additional
- Published
- 2005
- Full Text
- View/download PDF
10. Research on the Discoloration of Nori (Porphyra) and Characteristics of Water Quality Using the Numerical Model.
- Author
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Suenaga, Y., Tsunezawa, Y., Matsuoka, S., Yoshimatsu, S., Ichimi, K., Fujiwara, M., Yamaga, K., and Tada, K.
- Published
- 2006
- Full Text
- View/download PDF
11. LAMINAR-TURBULENT TRANSITION OF FLOW IN A HEATED HORIZONTAL TUBE
- Author
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Koizumi, Hiroyoshi, primary and Yamaga, K., additional
- Published
- 1998
- Full Text
- View/download PDF
12. BIOCHEMISTRY OF THE GENE PRODUCTS FROM MURINE MHC MUTANTS.
- Author
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Nairn, R., Yamaga, K., and Nathenson, S. G.
- Subjects
- *
H-2 locus , *BIOCHEMISTRY , *GENETIC mutation , *GENES , *IMMUNOLOGY - Abstract
Assesses the biochemistry of the gene products of the H-2 mutants. Methods for biochemical analysis of the H-2 mutants; Functional and structural properties of the gene products; Changes in the reactivity in various immunological test systems.
- Published
- 1980
- Full Text
- View/download PDF
13. Peptides from the Site of Chicken Anti-DNP Antibodies.
- Author
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Benedict, A. A., Roholt, O. A., Yamaga, K., and Pressman, D.
- Published
- 1972
- Full Text
- View/download PDF
14. Merozoite surface coat precursor protein completely protects Aotus monkeys against Plasmodium falciparum malaria.
- Author
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Siddiqui, W A, Tam, L Q, Kramer, K J, Hui, G S, Case, S E, Yamaga, K M, Chang, S P, Chan, E B, and Kan, S C
- Abstract
Groups of Aotus (owl) monkeys were immunized with either the Plasmodium falciparum merozoite surface-coat precursor protein and its processing fragments or a complex of high molecular mass rhoptry proteins and challenged with a lethal infection of the homologous P. falciparum Uganda Palo Alto (FUP) strain. No patent parasitemia could be detected on thick blood films of monkeys immunized with the merozoite surface antigens; however, only one of three monkeys immunized with the rhoptry proteins was partially protected, while two required drug therapy. The experiment clearly demonstrates that the merozoite surface-coat precursor protein can completely protect Aotus monkeys against a lethal infection of the human malaria parasite.
- Published
- 1987
- Full Text
- View/download PDF
15. Surgical treatment strategy for patients with stage IV hepatocellular carcinoma
- Author
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Shimada, M., Takenaka, K., Kawahara, N., Kajiyama, K., Yamamoto, K., Shirabe, K., Nishizaki, T., Yamaga, K., and Sugimachi, K.
- Abstract
Background. This study was conducted to identify the prognostic indicators for patients with stage IV hepatocellular carcinoma (HCC), as well as to clarify the strategy of surgical treatment for those patients. Methods. Forty-six patients with stage IV HCC were included in this study. Prognostic factors were univariately and multivariately analyzed. Furthermore, the significance of intraoperative treatment for residual tumors was investigated in patients with an absolute noncurative operation. Results. The poor prognostic factors were as follows: host factors, Child's classification of B and C and immunosuppressive acidic protein level of greater than 400 @mg/ml; tumor factors, tumor diameter of greater than 5 cm, poorly differentiated histologic features, positive portal vein invasion, and intrahepatic metastases involving more than three segments; others, an absolute noncurative operation and no preoperative treatment. Tumor diameter of more than 5 cm was then suggested to be an independent prognostic indicator. Survival of patients with stage IV-A HCC who underwent a curative operation was similar to that of those with stages I to III HCC. Furthermore, the survival of patients with Stage IV-A who had an absolute noncurative operation but underwent either intraoperative microwave coagulation or ethanol injection to the residual HCCs was not statistically different from that of those with a curative operation. Conclusions. Therefore for stage IV-A HCC surgical treatment is considered to be both useful and the first choice of treatment when all the tumors in the liver can be removed or when the residual tumors can be treated during operation by either microwave coagulation or ethanol injection as a result of an incomplete removal of the tumors.
- Published
- 1996
- Full Text
- View/download PDF
16. Induction of protective immunity to monoclonal-antibody-defined Plasmodium falciparum antigens requires strong adjuvant in Aotus monkeys
- Author
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Siddiqui, W A, Tam, L Q, Kan, S C, Kramer, K J, Case, S E, Palmer, K L, Yamaga, K M, and Hui, G S
- Abstract
Monoclonal antibodies to the major Plasmodium falciparum merozoite surface coat and rhoptry antigens were produced. A combination of the affinity-purified polypeptides with Freund complete adjuvant which was given three times completely protected an Aotus lemurinus azure (karotype VI) monkey against homologous challenge; however, immunization with the same polypeptides with a muramyl dipeptide derivative [MDP-Lys(L18)] did not protect a second Aotus monkey, even though comparable high antibody titers were induced.
- Published
- 1986
- Full Text
- View/download PDF
17. Plasmodium falciparum: protein antigens identified by analysis of serum samples from vaccinated Aotus monkeys
- Author
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Kan, S C, Yamaga, K M, Kramer, K J, Case, S E, and Siddiqui, W A
- Abstract
Serum samples from Aotus trivirgatus subsp. griseimembra monkeys obtained at different stages of a vaccination experiment were analyzed for total antibody titer to Plasmodium falciparum and were used for identifying protective antigens of the human malaria parasite. Total malarial antibody titers were higher in serum samples from protected monkeys (vaccinated with antigen in an adjuvant) than in those from unprotected monkeys (vaccinated with either antigen or adjuvant only). Parasite proteins were labeled with [3H]isoleucine, solubilized with nonionic detergent, and reacted with immune Aotus sera. Immunoprecipitates obtained were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and fluorography. Thirteen protein antigen bands in the molecular weight range 73,000 to 180,000 were resolved. Serum samples obtained from protected Aotus monkeys reacted more intensely with these proteins than samples from unprotected monkeys did. Evidence is presented that the protective antigen is not a single, normally nonimmunogenic, protein that is recognized only in protected monkeys. Rather, the present data indicate that a heightened immune response to multiple proteins correlated with in vivo protection to P. falciparum in Aotus monkeys. This finding may have a significant bearing on strategies for the development of a human P. falciparum vaccine.
- Published
- 1984
- Full Text
- View/download PDF
18. Microbial degradation of a soap-based fire-fighting agent in activated sludge
- Author
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Mizuki, H., Toyomura, M., Uezu, K., Hidenari Yasui, Kawano, T., Akiba, I., Kawahara, T., Hatae, S., Sakamoto, N., Akiyama, M., Mizota, C., Umeki, H., and Yamaga, K.
19. Flux growth of α-Fe2O3 by fast cooling from low soaking temperatures
- Author
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Ito, S., primary, Yamaga, K., additional, and Yoneda, N., additional
- Published
- 1982
- Full Text
- View/download PDF
20. A Tester for Measuring the Eveness of Lap in Widthwise Direction
- Author
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Ozawa, G., primary and Yamaga, K., additional
- Published
- 1962
- Full Text
- View/download PDF
21. Winding Tension on Fly -frame
- Author
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Osawa, G., primary and Yamaga, K., additional
- Published
- 1965
- Full Text
- View/download PDF
22. The Damage of Viscose Staple Fibers in Cotton Spinning Process
- Author
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Yamaga, K., primary
- Published
- 1957
- Full Text
- View/download PDF
23. 104. On the Target Points of Stereotaxic Operation for Globus Pallidus Some Thalamic Nuclei
- Author
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HORI, Y., primary, UTSUMI, S., additional, KANAZAWA, K., additional, YAMAGA, K., additional, KAMATA, K., additional, KYOI, K., additional, MIYATAKA, S., additional, MIYAMOTO, S., additional, and ENOKI, M., additional
- Published
- 1962
- Full Text
- View/download PDF
24. Spontaneous Hepatic Hydrothorax-genesis of pleural effusion complicating liver cirrhosis, Report 2.
- Author
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Okuda, K, primary, Tanikawa, K, additional, Shimokawa, Y, additional, Watanabe, H, additional, Yoshimura, K, additional, Urakawa, S, additional, and Yamaga, K, additional
- Published
- 1968
- Full Text
- View/download PDF
25. Flux growth of α-Fe 2O 3 by fast cooling from low soaking temperatures
- Author
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Ito, S., Yamaga, K., and Yoneda, N.
- Published
- 1982
- Full Text
- View/download PDF
26. In vivo fertilization improved the cryotolerance and developmental ability of vitrified-warmed rat fertilized oocytes.
- Author
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Ishizuka Y, Nakao S, Kamisako T, Yamaga K, Nakagata N, Ishizaki H, and Takeo T
- Subjects
- Animals, Rats, Female, Male, Rats, Sprague-Dawley, Embryo Transfer methods, Embryonic Development, Fertilization, Cryopreservation methods, Oocytes cytology, Oocytes physiology, Fertilization in Vitro methods, Vitrification, Rats, Inbred F344
- Abstract
The cryopreservation of rat embryos is useful for efficiently archiving rat resources in bioresource repositories. The cryopreserved fertilized oocytes can be quickly reanimated to rats with homozygous mutations using embryo transfer. In addition, cryopreserved rat fertilized oocytes are easier to transport than live animals. Before cryopreservation, fertilized oocytes are obtained by mating or in vitro fertilization. However, it is not clear which fertilized oocytes are most suited to cryopreservation. In this study, we performed a systematic comparison of the fertilizing ability, cryotolerance, and developmental ability of cryopreserved fertilized oocytes at the pronuclear stage produced either by mating (in vivo) or in vitro fertilization (in vitro) in SD and F344 rats. In vivo-fertilized oocytes had higher cryotolerance and developmental ability than in vitro-fertilized oocytes in SD and F344 rats. Furthermore, the fertilization ability, cryotolerance, and developmental ability of vitrified-warmed fertilized oocytes differed between SD and F344 rats. In conclusion, our study suggests that in vivo-fertilized rat oocytes were more suitable for cryopreservation. Our protocol provides an optimized system for the management of rat colonies using fertilized oocytes cryopreservation and contributes to the 3Rs principle by reducing the number of animals used for research., (© 2024. The Author(s).)
- Published
- 2024
- Full Text
- View/download PDF
27. Sclerotherapy for Aggressive Vertebral Hemangioma with Severe Bone Destruction: A 5-Year Analysis.
- Author
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Takeda C, Tanishima S, Ohuchi Y, Mihara T, Yamaga K, Yoshida M, and Nagashima H
- Abstract
Competing Interests: Conflicts of Interest: The authors declare that there are no relevant conflicts of interest.
- Published
- 2024
- Full Text
- View/download PDF
28. High-concentration bovine serum albumin enhances fertilization ability of cold-stored rat sperm.
- Author
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Yamaga K, Nakao S, Mikoda N, Sztein JM, Nakagata N, and Takeo T
- Subjects
- Animals, Rats, Male, Semen, Spermatozoa, Sperm Capacitation, Serum Albumin, Bovine pharmacology, Fertilization in Vitro methods
- Abstract
Cold transport of the cauda epididymides is a useful technique for shipping laboratory rat sperm. Cold transport of rat sperm avoids potential risks of microbiological infection, animal escape or death, and animal welfare issues. Previously, we reported that a cold-storage solution containing dimethyl sulfoxide and quercetin maintained the fertility of cold-stored rat sperm. However, cold-stored rat sperm exhibited a decreased fertilization rate after 24-h storage. To recover the fertility of cold-stored sperm, we focused on the effects of bovine serum albumin (BSA), a cholesterol acceptor that induces sperm capacitation. We sought to determine the optimal concentration of BSA in fertilization medium based on the fertility of cold-stored rat sperm. High concentrations of BSA (40 mg/ml) enhanced the fertilization rate of cold-stored rat sperm and maintained sperm fertility for 144 h. Embryos derived from cold-stored and BSA-treated sperm normally developed into pups after embryo transfer. In summary, high BSA concentrations enhanced the fertility of cold-stored rat sperm and prolonged the storage period to 144 h, thereby expanding the transportable region for genetically engineered rats.
- Published
- 2024
- Full Text
- View/download PDF
29. Time elapsed between ovulation and insemination determines the quality of fertilized rat oocytes.
- Author
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Nakagata N, Nakao S, Mikoda N, Yamaga K, and Takeo T
- Subjects
- Female, Male, Rats, Humans, Animals, Oocytes, Cryopreservation methods, Ovulation, Insemination, Semen, Fertilization in Vitro methods
- Abstract
Genetically modified rats are valuable models in human disease research. We recently developed an improved system for rat sperm cryopreservation and in vitro fertilization (IVF) that facilitates the efficient production and preservation of genetically modified rats. In the IVF procedure performed using frozen-thawed rat sperm, the IVF schedule is fixed to ensure timely hormone administration and oocyte collection. To enhance the flexibility of the IVF schedule, possible periods of postovulated rat oocytes with normal fertility and developmental abilities should be determined. Therefore, in this study, we examined the fertilization and developmental ability of incubated oocytes 1-13 h after oocyte collection at 9:00 AM. The fertilization rate decreased 7 h after oocyte collection, and abnormally fertilized oocytes appeared 10 h after oocyte collection. The developmental rate also decreased 7 h after oocyte collection; however, live pups were obtained from oocytes 12 h after oocyte collection. In summary, ovulated rat oocytes exhibited a high developmental ability after IVF for up to 4 h after oocyte collection.
- Published
- 2024
- Full Text
- View/download PDF
30. An unresectable osteoblastoma of the axis controlled with denosumab.
- Author
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Yamaga K, Kuwamoto S, Tanishima S, Yamashita H, Asano N, Matsushita M, Akahori K, Osaki M, Hisaoka M, and Nagashima H
- Subjects
- Humans, Denosumab therapeutic use, Osteoblastoma diagnostic imaging, Osteoblastoma drug therapy, Osteoblastoma surgery, Bone Density Conservation Agents therapeutic use, Bone Neoplasms diagnostic imaging, Bone Neoplasms drug therapy
- Abstract
Competing Interests: Declaration of competing interest The authors declare that they have no conflict of interest.
- Published
- 2024
- Full Text
- View/download PDF
31. Signalling by senescent melanocytes hyperactivates hair growth.
- Author
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Wang X, Ramos R, Phan AQ, Yamaga K, Flesher JL, Jiang S, Oh JW, Jin S, Jahid S, Kuan CH, Nguyen TK, Liang HY, Shettigar NU, Hou R, Tran KH, Nguyen A, Vu KN, Phung JL, Ingal JP, Levitt KM, Cao X, Liu Y, Deng Z, Taguchi N, Scarfone VM, Wang G, Paolilli KN, Wang X, Guerrero-Juarez CF, Davis RT, Greenberg EN, Ruiz-Vega R, Vasudeva P, Murad R, Widyastuti LHP, Lee HL, McElwee KJ, Gadeau AP, Lawson DA, Andersen B, Mortazavi A, Yu Z, Nie Q, Kunisada T, Karin M, Tuckermann J, Esko JD, Ganesan AK, Li J, and Plikus MV
- Subjects
- Animals, Mice, Hair Follicle cytology, Hair Follicle physiology, Hyaluronan Receptors metabolism, Nevus metabolism, Nevus pathology, Osteopontin metabolism, Stem Cells cytology, Hair cytology, Hair growth & development, Melanocytes cytology, Melanocytes metabolism, Signal Transduction
- Abstract
Niche signals maintain stem cells in a prolonged quiescence or transiently activate them for proper regeneration
1 . Altering balanced niche signalling can lead to regenerative disorders. Melanocytic skin nevi in human often display excessive hair growth, suggesting hair stem cell hyperactivity. Here, using genetic mouse models of nevi2,3 , we show that dermal clusters of senescent melanocytes drive epithelial hair stem cells to exit quiescence and change their transcriptome and composition, potently enhancing hair renewal. Nevus melanocytes activate a distinct secretome, enriched for signalling factors. Osteopontin, the leading nevus signalling factor, is both necessary and sufficient to induce hair growth. Injection of osteopontin or its genetic overexpression is sufficient to induce robust hair growth in mice, whereas germline and conditional deletions of either osteopontin or CD44, its cognate receptor on epithelial hair cells, rescue enhanced hair growth induced by dermal nevus melanocytes. Osteopontin is overexpressed in human hairy nevi, and it stimulates new growth of human hair follicles. Although broad accumulation of senescent cells, such as upon ageing or genotoxic stress, is detrimental for the regenerative capacity of tissue4 , we show that signalling by senescent cell clusters can potently enhance the activity of adjacent intact stem cells and stimulate tissue renewal. This finding identifies senescent cells and their secretome as an attractive therapeutic target in regenerative disorders., (© 2023. The Author(s).)- Published
- 2023
- Full Text
- View/download PDF
32. Double mutation of claudin-1 and claudin-3 causes alopecia in infant mice.
- Author
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Suzuki K, Yamaga K, Tokumasu R, Katsuno T, Tanaka H, Chiba S, Yagi T, Katayama I, Tamura A, Murota H, and Tsukita S
- Subjects
- Animals, Mice, Claudin-1 genetics, Claudin-1 metabolism, Claudin-3 genetics, Claudin-3 metabolism, Claudin-4 metabolism, Mutation, Aging, Alopecia genetics
- Abstract
Hair follicles (HFs) undergo cyclic phases of growth, regression, and rest in association with hair shafts to maintain the hair coat. Nonsense mutations in the tight junction protein claudin (CLDN)-1 cause hair loss in humans. Therefore, we evaluated the roles of CLDNs in hair retention. Among the 27 CLDN family members, CLDN1, CLDN3, CLDN4, CLDN6, and CLDN7 were expressed in the inner bulge layer, isthmus, and sebaceous gland of murine HFs. Hair phenotypes were observed in Cldn1 weaker knockdown and Cldn3-knockout (Cldn1
Δ/Δ Cldn3-/- ) mice. Although hair growth was normal, Cldn1Δ/Δ Cldn3-/- mice showed striking hair loss in the first telogen. Simultaneous deficiencies in CLDN1 and CLDN3 caused abnormalities in telogen HFs, such as an aberrantly layered architecture of epithelial cell sheets in bulges with multiple cell layers, mislocalization of bulges adjacent to sebaceous glands, and dilated hair canals. Along with the telogen HF abnormalities, which shortened the hair retention period, there was an enhanced proliferation of the epithelium surrounding HFs in Cldn1Δ/Δ Cldn3-/- mice, causing accelerated hair regrowth in adults. Our findings suggested that CLDN1 and CLDN3 may regulate hair retention in infant mice by maintaining the appropriate layered architecture of HFs, a deficiency of which can lead to alopecia., (© 2023 New York Academy of Sciences.)- Published
- 2023
- Full Text
- View/download PDF
33. Observation of the in vitro fertilization process in living oocytes using frozen-thawed sperm in rats.
- Author
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Nakagata N, Nakao S, Mikoda N, Yamaga K, and Takeo T
- Subjects
- Male, Rats, Animals, Rats, Inbred F344, Rats, Wistar, Fertilization in Vitro veterinary, Oocytes, Spermatozoa, Sperm-Ovum Interactions, Cryopreservation veterinary, Sperm Motility, Semen
- Abstract
Previous studies have observed the fertilization process in rats using whole-mount preparation at different time-points after insemination. However, very few reports have described the various events during the fertilization process using an inverted microscope without whole-mount. Moreover, to the best of our knowledge, no reports have described the observation of changes in sperm motility associated with sperm penetration into oocytes. In this study, in vitro fertilization was performed using frozen-thawed sperm in various rat strains (SD, Wistar, LE, F344, and BN) and oocytes from the SD strain, and the process of sperm penetration into the oocytes and the subsequent development were observed. The sperm motility was assessed, and the correlation between the process of sperm penetration into the oocytes and sperm motility over time was examined. The motility of frozen sperm from the SD, Wistar, LE, and F344 increased at 2-3 h after thawing, at which time the sperm attached themselves to the zona pellucida. Sperm penetration into the zona pellucida occurred after 3-5 h, and pronuclei were formed in the cytoplasm of oocytes 5-9 h after insemination. The fertilities of frozen-thawed sperm from the SD, Wistar, LE, and F344 were 92.7%, 90.0%, 90.7%, and 68.7%, respectively. However, no increase in motility was observed after thawing of frozen sperm from the BN, and the fertility was only 21%. In addition, very few polyspermic oocytes were observed with use of frozen-thawed sperm of all strains. In summary, rats are suitable animals for the observation of sperm penetration into the oocytes, and we determined the timing of fertilization events in IVF using frozen-thawed rat sperm., Competing Interests: Declaration of competing interest The authors declare competing financial interests. The present study was partially supported by a grant of Kyudo Co. Ltd. N.M. is a member of Kyudo Co. Ltd. K.Y. received a grant from Kyudo Co. Ltd. N.N. is a member of collaboration laboratory between Kyudo Co. Ltd. and Kumamoto University. S.N. and T.T. report no financial interests., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2023
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34. Optimized protocols for sperm cryopreservation and in vitro fertilization in the rat.
- Author
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Takeo T, Nakao S, Mikoda N, Yamaga K, Maeda R, Tsuchiyama S, Nakatsukasa E, and Nakagata N
- Subjects
- Animals, Cryopreservation methods, Cryopreservation veterinary, Fertilization in Vitro methods, Fertilization in Vitro veterinary, Male, Rats, Spermatozoa, Semen, Serum Albumin, Bovine
- Abstract
Laboratory rats have been used in biomedical research for over 170 years. Recently, genome editing technology has facilitated the generation of genetically modified rats worldwide. This development has increased the demand for efficient preservation and production of rat resources. Sperm cryopreservation is the most efficient and robust means to archive genetic resources, and this technique reduces the number of animals required for colony management. Previously, we have reported a protocol for rat sperm cryopreservation and in vitro fertilization using frozen-thawed sperm. Here we describe an improved in vitro fertilization protocol to enhance the fertilization rate of cryopreserved sperm in major strains of rats. In this optimized protocol, treatment of frozen-thawed rat sperm with a high concentration of bovine serum albumin (40 mg/ml) results in a high in vitro fertilization rate. This protocol consists of three main steps: preparation of cryopreserved sperm, in vitro fertilization using cryopreserved sperm and embryo transfer. This process takes approximately 1 month to produce live pups from cryopreserved sperm. This protocol can be easily implemented by researchers and technicians with experience in reproductive engineering technology; it can also be used, albeit with some practice, by researchers and technicians who have no experience in reproductive techniques. This sperm cryopreservation and in vitro fertilization protocol for rats will provide an efficient system for the archiving and production of genetically modified rats for the transgenic community., (© 2022. Springer Nature America, Inc.)
- Published
- 2022
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35. Quercetin-treated rat sperm enables refrigerated transport with motility and fertility for five days.
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Yamaga K, Nakao S, Mikoda N, Yoshimoto H, Nakatsukasa E, Nakagata N, and Takeo T
- Subjects
- Animals, Embryo Transfer, Fertility, Fertilization in Vitro, Male, Rats, Time Factors, Cryopreservation methods, Dimethyl Sulfoxide pharmacology, Quercetin pharmacology, Semen Preservation methods, Sperm Motility drug effects, Spermatozoa drug effects, Spermatozoa physiology
- Abstract
Shipment of laboratory rats between animal facilities is frequently performed using special containers. However, the shipment of live animals is associated with potential risks of infectious diseases, escape and death during shipment and animal welfare issues. The transport of cold-stored sperm avoids such risks; however, there have been no reports on cold storage of rat sperm. We previously reported that dimethyl sulfoxide (DMSO) and quercetin maintained the motility and fertilising abilities of cold-stored mouse sperm stored for 10 days. The present study investigated the efficacy of DMSO and quercetin in the cold storage of rat sperm. Quercetin maintained motility and fertility of cold-stored rat sperm stored for 5 days. After in vitro fertilisation using cold-stored sperm, pronuclear and two-cell embryos developed normally to pups following embryo transfer. Therefore, we demonstrated that live pups could be obtained from sperm transported using the cold-storage system. We conclude that cold storage of rat sperm may provide an efficient system for transporting rat resources as an alternative to shipping live animals., (© 2021. The Author(s).)
- Published
- 2021
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36. YAP-mediated mechanotransduction tunes the macrophage inflammatory response.
- Author
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Meli VS, Atcha H, Veerasubramanian PK, Nagalla RR, Luu TU, Chen EY, Guerrero-Juarez CF, Yamaga K, Pandori W, Hsieh JY, Downing TL, Fruman DA, Lodoen MB, Plikus MV, Wang W, and Liu WF
- Subjects
- Extracellular Matrix metabolism, Humans, Inflammation metabolism, Macrophages, Mechanotransduction, Cellular physiology, YAP-Signaling Proteins
- Abstract
Macrophages are innate immune cells that adhere to the extracellular matrix within tissues. However, how matrix properties regulate their function remains poorly understood. Here, we report that the adhesive microenvironment tunes the macrophage inflammatory response through the transcriptional coactivator YAP. We find that adhesion to soft hydrogels reduces inflammation when compared to adhesion on stiff materials and is associated with reduced YAP expression and nuclear localization. Substrate stiffness and cytoskeletal polymerization, but not adhesive confinement nor contractility, regulate YAP localization. Furthermore, depletion of YAP inhibits macrophage inflammation, whereas overexpression of active YAP increases inflammation. Last, we show in vivo that soft materials reduce expression of inflammatory markers and YAP in surrounding macrophages when compared to stiff materials. Together, our studies identify YAP as a key molecule for controlling inflammation and sensing stiffness in macrophages and may have broad implications in the regulation of macrophages in health and disease., (Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).)
- Published
- 2020
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37. Successful Treatment with Denosumab of a Giant Cell Tumor of Bone in the Iliac Bone of an 84-Year-Old Man.
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Yamaga K, Kuwamoto S, Mukunoki D, Osaki M, and Nagashima H
- Abstract
We report a case of GCTB in an 84-year-old Japanese man who had a tumor in his left iliac bone and was treated safely with denosumab. The patient noticed a painful mass, with gradual enlargement, in his left low back next to the iliac region. Magnetic resonance imaging revealed that the tumor measured 94 × 66 × 90 mm and was located in the left iliac bone. Histologically, the tumor was composed of proliferative oval-shaped mononuclear cells, admixed with large number of osteoclast-like giant cells. Immunohistochemically, a strong positivity for histone 3.3 G34W mutant protein was observed in the nuclei of the mononuclear cells, confirming the diagnosis of GCTB. Because it was considered as unresectable tumor, the patient was treated with denosumab without any side effects., Competing Interests: The authors declare no conflict of interest., (©2020 Tottori University Medical Press.)
- Published
- 2020
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38. Considering the Better Donor-side in Reconstruction of Composite Radius After Resection of Spindle Cell Sarcoma Using Free Fibular Osteocutaneous Flap: A Case Report.
- Author
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Yagi S, Suyama Y, Fukuoka K, Morita M, Yamaga K, and Nagashima H
- Abstract
Reconstruction of bone and soft-tissue defects in the forearm is a surgery that often proves unsuccessful. Free fibular osteocutaneous flaps are a useful material for reconstruction that enable simultaneous reconstruction of bone, skin, and soft tissues. However, in free fibular osteocutaneous flaps, the fibula, skin, and vascular pedicle are tightly bound together by the posterior intermuscular septum and the perforators that pass through the septum, giving the disadvantage of a low degree of freedom when setting these structures in place. We take into account the 3-dimensional structure of the free skin flap when selecting which lower leg to use as the donor. We report here the case of a 61-year-old man with defects in the radius, skin, and soft tissues after resection of spindle cell carcinoma of the right forearm, which was reconstructed using a free fibular osteocutaneous flap harvested from the left lower leg. Two years postoperatively, recovery has been uneventful with no complications. Donor-side selection of free fibular osteocutaneous flap is an important factor for safely completing composite radius reconstruction., Competing Interests: The authors declare no conflict of interest., (©2020 Tottori University Medical Press.)
- Published
- 2020
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39. Female child with hematidrosis of the palm: Case report and published work review.
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Murota H, Kotobuki Y, Yamaga K, and Yoshioka Y
- Subjects
- Biopsy, Child, Female, Hand pathology, Hemorrhage etiology, Humans, Hyperhidrosis etiology, Rare Diseases etiology, Skin pathology, Water administration & dosage, Hemorrhage diagnosis, Hyperhidrosis diagnosis, Iontophoresis methods, Rare Diseases diagnosis, Sweat chemistry, Sweat cytology
- Abstract
Hematidrosis is a rare disorder involving spontaneous excretion of sweat contaminated by blood cells. We report the case of a 6-year-old girl with hematidrosis from her palms with no underlying disease or psychotic disorder. Before the onset of this symptom, the patient was given an indoor horizontal exercise bar with which she had been frequently playing. This symptom appeared without apparent triggers and was not associated with subjective symptoms. To examine her hematidrosis, metabolites in the red bodily fluid were analyzed using nuclear magnetic resonance analysis. We found the fluid had a metabolome profile similar to that of eccrine sweat. Pathological analysis revealed no abnormal findings, including expression of the tight junction protein claudin 3. Her symptom decreased after treatment with tap-water iontophoresis. Here, we describe our case and discuss its etiology by reviewing previous reports., (© 2019 Japanese Dermatological Association.)
- Published
- 2020
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40. Why does sweat lead to the development of itch in atopic dermatitis?
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Murota H, Yamaga K, Ono E, Murayama N, Yokozeki H, and Katayama I
- Subjects
- Humans, Dermatitis, Atopic, Pruritus etiology, Sweat physiology
- Abstract
Sweating plays an important role in maintaining temperature homeostasis in humans. However, under certain circumstances, sweat can cause itching. For example, when excessive sweat accumulates on the skin surface for a long period, miliaria can develop and cause itching. Subjects with dermatoses, such as atopic dermatitis (AD), suffer from itch when exposed to heat or psychological stresses, which are also known perspiration stimuli. Recently, some mechanisms of sweat-induced itch have been revealed. For instance, attenuated sweating ability is observed in subjects with AD, causing heat retention, skin dryness, and high susceptibility to itch. Furthermore, the decreased tight junction of the sweat gland in AD leads to sweat leakage in the dermis, which could be designated as a "sweat endocrine response" and may be the cause of tingling itch during sweating. Additionally, metabolomic analysis of sweat from patients with AD revealed that glucose concentration in sweat increases according to disease severity. Sweat with elevated glucose concentration retards the recovery of the damaged skin barrier and may promote itching. This viewpoint essay outlines the relationship between sweat and itch based on recent evidence., (© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2019
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41. Single-cell analysis reveals fibroblast heterogeneity and myeloid-derived adipocyte progenitors in murine skin wounds.
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Guerrero-Juarez CF, Dedhia PH, Jin S, Ruiz-Vega R, Ma D, Liu Y, Yamaga K, Shestova O, Gay DL, Yang Z, Kessenbrock K, Nie Q, Pear WS, Cotsarelis G, and Plikus MV
- Subjects
- Animals, Blotting, Western, Cells, Cultured, Female, Male, Mice, Sequence Analysis, RNA, Stem Cells metabolism, Wound Healing physiology, Adipocytes cytology, Adipocytes metabolism, Fibroblasts cytology, Fibroblasts metabolism, Single-Cell Analysis methods, Skin cytology, Stem Cells cytology
- Abstract
During wound healing in adult mouse skin, hair follicles and then adipocytes regenerate. Adipocytes regenerate from myofibroblasts, a specialized contractile wound fibroblast. Here we study wound fibroblast diversity using single-cell RNA-sequencing. On analysis, wound fibroblasts group into twelve clusters. Pseudotime and RNA velocity analyses reveal that some clusters likely represent consecutive differentiation states toward a contractile phenotype, while others appear to represent distinct fibroblast lineages. One subset of fibroblasts expresses hematopoietic markers, suggesting their myeloid origin. We validate this finding using single-cell western blot and single-cell RNA-sequencing on genetically labeled myofibroblasts. Using bone marrow transplantation and Cre recombinase-based lineage tracing experiments, we rule out cell fusion events and confirm that hematopoietic lineage cells give rise to a subset of myofibroblasts and rare regenerated adipocytes. In conclusion, our study reveals that wounding induces a high degree of heterogeneity among fibroblasts and recruits highly plastic myeloid cells that contribute to adipocyte regeneration.
- Published
- 2019
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42. Sweat in the pathogenesis of atopic dermatitis.
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Murota H, Yamaga K, Ono E, and Katayama I
- Subjects
- Humans, Dermatitis, Atopic immunology, Dermatitis, Atopic physiopathology, Sweat chemistry, Sweat immunology, Sweat physiology
- Abstract
Sweat is a transparent hypotonic body fluid made from eccrine sweat glands. Various ingredients contained in sweat are involved in a broad sense in skin homeostasis including temperature regulation, skin moisture, and immune functions. Thus, sweat plays a major role in maintaining skin homeostasis. Therefore, abnormal sweating easily compromises human health. For example, in atopic dermatitis (AD), perspiration stagnation accompanying sweat tube or sweat pore blockage, leakage of perspiration from the sweat gland to the outside tissue, and impaired secretion of sweat from the sweat gland are confirmed. In recent years, the hypothesis that atopic dermatitis is a sweat stasis syndrome has been clarified by the establishment of a sweat and sweat gland dynamic analysis technique. Secretion of sweat and leakage into tissues is caused by dermatitis and is thought to promote itching. Furthermore, from the metabolomic analysis of sweat of patients with atopic dermatitis, it was confirmed that the glucose concentration in AD sweat increased according to severity and skin phenotype, suggesting that elevated glucose affected the homeostasis of the skin. Multifaceted analyses of sweat from subjects with AD have revealed new aspects of the pathology, and appropriate measures to treat sweat can be expected to contribute to long-term control of AD., (Copyright © 2018 Japanese Society of Allergology. Production and hosting by Elsevier B.V. All rights reserved.)
- Published
- 2018
- Full Text
- View/download PDF
43. Use of Fat Grafts for Stabilizing Microvascular Pedicle Geometry in Lower Limb Reconstruction.
- Author
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Yagi S, Suyama Y, Yamaga K, Morita M, and Fukuoka K
- Published
- 2018
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- View/download PDF
44. Claudin-3 Loss Causes Leakage of Sweat from the Sweat Gland to Contribute to the Pathogenesis of Atopic Dermatitis.
- Author
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Yamaga K, Murota H, Tamura A, Miyata H, Ohmi M, Kikuta J, Ishii M, Tsukita S, and Katayama I
- Subjects
- Acetylcholine pharmacology, Adult, Animals, Claudin-3 genetics, Female, Healthy Volunteers, Humans, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Sweat metabolism, Sweat Glands cytology, Sweat Glands drug effects, Sweating drug effects, Sweating physiology, Tight Junctions metabolism, Water Loss, Insensible physiology, Young Adult, Claudin-3 metabolism, Dermatitis, Atopic pathology, Sweat Glands pathology, Tight Junctions pathology
- Abstract
The transfer of sweat to the skin surface without leakage is important for the homeostatic regulation of skin and is impaired in atopic dermatitis. Although the precise composition of the leakage barrier remains obscure, there is a large contribution from claudins, the major components of tight junctions. In humans, claudin-1, -3, and -15 are expressed on sweat ducts, and claudin-3 and -10 are expressed on secretory coils. Although only two claudins are expressed in murine sweat glands, we found that the expression of claudin-3 is conserved. Atopic dermatitis lesional skin had decreased claudin-3 expression in sweat glands, which was accompanied by sweat leakage. This critical role in water barrier function was confirmed in Cldn3
-/- and Cldn3+/- mice and those with experimentally decreased claudin-3. Our results show the crucial role of claudin-3 in preventing sweat gland leakage and suggest that the pathogenesis of dermatoses accompanied by hypohidrosis involves abnormally decreased claudin-3., (Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.)- Published
- 2018
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- View/download PDF
45. [SWEAT PLAYS A ROLE IN IMMUNE PROTECTION].
- Author
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Murota H, Tahara M, Shindo S, Ono E, Yamaga K, and Katayama I
- Subjects
- Dermatitis, Atopic, Humans, Sweating, Sweat
- Published
- 2018
- Full Text
- View/download PDF
46. Detailed analysis of a superficial CD34-positive fibroblastic tumor: A case report and review of the literature.
- Author
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Yamaga K, Fujita A, Osaki M, Kuwamoto S, Ishiguro N, Yamamoto T, and Nagashima H
- Abstract
Superficial cluster of differentiation (CD)34-positive fibroblastic tumor (SCPFT) is a rare mesenchymal neoplasm of borderline malignancy. It is characterized by a superficial location, marked cellular pleomorphism, an extremely low incidence of mitotic figures, and strong CD34 immunohistochemical positivity. As SCPFT is a recently described neoplasm, its characteristics are yet to be fully elucidated. To the best of our knowledge, no detailed studies regarding the imaging findings and cytogenetic analyses of SCPFTs exist. The present study describes a typical case of an 18-year-old man who developed an SCPFT measuring 87×70×80 mm in the subcutaneous adipose tissue of his right thigh. Computed tomography (CT) revealed a well-marginated tumor without calcification, and the enhancement on CT was weak. The tumor demonstrated abnormal uptake on 2-(18F) fluoro-2-deoxy-D-glucose positron emission tomography (PET), with a maximum standardized uptake value of 2.57. Magnetic resonance imaging (MRI) revealed a clearly defined tumor that exhibited homogeneous low signal intensity on T1-weighted imaging and high signal intensity on T2-weighted imaging, with small lobulated structures. Histopathologically, the tumor was composed of irregular spindle-to-oval-shaped cells with eosinophilic glassy cytoplasm and hyperchromatic, bizarre and pleomorphic nuclei that frequently exhibited intranuclear pseudoinclusions. Immunohistochemically, the tumor cells were diffusely and strongly positive for CD34. The Mindbomb E3 ubiquitin protein ligase 1 labeling index was 8.6%. Ultrastructurally, the tumor cells exhibited irregular or convoluted nuclei with abundant euchromatin-prominent nucleoli. The cytoplasmic organelles consisted of scattered, abundant rough endoplasmic reticulum, mitochondria, lysosomes, ribosomal rosettes and aggregated lipid globules. Of 18 metaphase cells identified, 2 demonstrated translocation between chromosomes 2 and 5 in cytogenetic studies. To the best of our knowledge, this is the first study describing imaging data (CT, MRI and PET-CT) and chromosomal aberrations for SCPFT.
- Published
- 2017
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47. Health-related behaviors associated with subjective sleep insufficiency in Japanese workers: A cross-sectional study.
- Author
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Kageyama M, Odagiri K, Mizuta I, Yamamoto M, Yamaga K, Hirano T, Onoue K, and Uehara A
- Subjects
- Adolescent, Adult, Aged, Body Weight, Cross-Sectional Studies, Employment, Exercise psychology, Feeding Behavior, Female, Humans, Japan epidemiology, Logistic Models, Male, Middle Aged, Risk Factors, Sex Distribution, Smoking epidemiology, Smoking psychology, Surveys and Questionnaires, Young Adult, Health Behavior, Sleep Deprivation epidemiology, Sleep Deprivation etiology
- Abstract
Objectives: Sleep disturbances are related to somatic and mental disorders, industrial accidents, absenteeism, and retirement because of disability. We aimed to identify health-related behaviors associated with subjective sleep insufficiency in Japanese workers., Methods: This cross-sectional study included 5,297 employees (mean age: 43.6±11.3 years; 4,039 men). Multiple logistic regression analysis was used to identify health-related behaviors associated with subjective sleep insufficiency., Results: Overall, 28.2% of participants experienced subjective sleep insufficiency. There was a significant difference between the genders in the proportion of participants with subjective sleep insufficiency (male: 26.4%; female: 34.3%; p<0.001). Multiple logistic regression analysis revealed that being a female or ≥40 years, experiencing a weight change of ≥3 kg during the preceding year, not exercising regularly, not walking quickly, and eating a late-evening or fourth meal were associated with subjective sleep insufficiency. After stratifying by gender, age ≥40 years, not exercising regularly, and eating a late-evening or fourth meal were significantly associated with subjective sleep insufficiency in both genders. Not walking quickly, experiencing a weight change, and eating quickly were positively associated with subjective sleep insufficiency only for males. Females who did not engage in physical activity were more likely to have experienced subjective sleep insufficiency, but this relationship was not observed in males., Conclusions: The results indicated that certain health-related behaviors, specifically not exercising regularly and nocturnal eating habits, were associated with subjective sleep insufficiency in a group of Japanese workers.
- Published
- 2017
- Full Text
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48. Dose-dependent role of claudin-1 in vivo in orchestrating features of atopic dermatitis.
- Author
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Tokumasu R, Yamaga K, Yamazaki Y, Murota H, Suzuki K, Tamura A, Bando K, Furuta Y, Katayama I, and Tsukita S
- Subjects
- Adolescent, Adult, Age Factors, Aged, Animals, Claudin-1 genetics, Dermatitis, Atopic metabolism, Dermatitis, Atopic pathology, Epidermis pathology, Female, Humans, Male, Mice, Middle Aged, Permeability, Phenotype, Young Adult, Claudin-1 metabolism, Dermatitis, Atopic etiology, Disease Models, Animal, Epidermis immunology
- Abstract
Atopic dermatitis (AD) is a chronic inflammatory skin disease in humans. It was recently noted that the characteristics of epidermal barrier functions critically influence the pathological features of AD. Evidence suggests that claudin-1 (CLDN1), a major component of tight junctions (TJs) in the epidermis, plays a key role in human AD, but the mechanism underlying this role is poorly understood. One of the main challenges in studying CLDN1's effects is that Cldn1 knock-out mice cannot survive beyond 1 d after birth, due to lethal dehydration. Here, we established a series of mouse lines that express Cldn1 at various levels and used these mice to study Cldn1's effects in vivo. Notably, we discovered a dose-dependent effect of Cldn1's expression in orchestrating features of AD. In our experimental model, epithelial barrier functions and morphological changes in the skin varied exponentially with the decrease in Cldn1 expression level. At low Cldn1 expression levels, mice exhibited morphological features of AD and an innate immune response that included neutrophil and macrophage recruitment to the skin. These phenotypes were especially apparent in the infant stages and lessened as the mice became adults, depending on the expression level of Cldn1 Still, these adult mice with improved phenotypes showed an enhanced hapten-induced contact hypersensitivity response compared with WT mice. Furthermore, we revealed a relationship between macrophage recruitment and CLDN1 levels in human AD patients. Our findings collectively suggest that CLDN1 regulates the pathogenesis, severity, and natural course of human AD.
- Published
- 2016
- Full Text
- View/download PDF
49. Report from the fourth international consensus meeting to harmonize core outcome measures for atopic eczema/dermatitis clinical trials (HOME initiative).
- Author
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Chalmers JR, Simpson E, Apfelbacher CJ, Thomas KS, von Kobyletzki L, Schmitt J, Singh JA, Svensson Å, Williams HC, Abuabara K, Aoki V, Ardeleanu M, Awici-Rasmussen M, Barbarot S, Berents TL, Block J, Bragg A, Burton T, Bjerring Clemmensen KK, Creswell-Melville A, Dinesen M, Drucker A, Eckert L, Flohr C, Garg M, Gerbens LA, Graff AL, Hanifin J, Heinl D, Humphreys R, Ishii HA, Kataoka Y, Leshem YA, Marquort B, Massuel MA, Merhand S, Mizutani H, Murota H, Murrell DF, Nakahara T, Nasr I, Nograles K, Ohya Y, Osterloh I, Pander J, Prinsen C, Purkins L, Ridd M, Sach T, Schuttelaar ML, Shindo S, Smirnova J, Sulzer A, Synnøve Gjerde E, Takaoka R, Vestby Talmo H, Tauber M, Torchet F, Volke A, Wahlgren CF, Weidinger S, Weisshaar E, Wollenberg A, Yamaga K, Zhao CY, and Spuls PI
- Subjects
- Checklist, Clinical Trials as Topic, Dermatologic Agents therapeutic use, Global Health, Humans, Long-Term Care, Patient Reported Outcome Measures, Quality of Life, Review Literature as Topic, Treatment Outcome, Dermatitis, Atopic therapy
- Abstract
This article is a report of the fourth meeting of the Harmonising Outcome Measures for Eczema (HOME) initiative held in Malmö, Sweden on 23-24 April 2015 (HOME IV). The aim of the meeting was to achieve consensus over the preferred outcome instruments for measuring patient-reported symptoms and quality of life for the HOME core outcome set for atopic eczema (AE). Following presentations, which included data from systematic reviews, consensus discussions were held in a mixture of whole group and small group discussions. Small groups were allocated a priori to ensure representation of different stakeholders and countries. Decisions were voted on using electronic keypads. For the patient-reported symptoms, the group agreed by vote that itch, sleep loss, dryness, redness/inflamed skin and irritated skin were all considered essential aspects of AE symptoms. Many instruments for capturing patient-reported symptoms were discussed [including the Patient-Oriented SCOring Atopic Dermatitis index, Patient-Oriented Eczema Measure (POEM), Self-Administered Eczema Area and Severity Index, Itch Severity Scale, Atopic Dermatitis Quickscore and the Nottingham Eczema Severity Score] and, by consensus, POEM was selected as the preferred instrument to measure patient-reported symptoms. Further work is needed to determine the reliability and measurement error of POEM. Further work is also required to establish the importance of pain/soreness and the importance of collecting information regarding the intensity of symptoms in addition to their frequency. Much of the discussion on quality of life concerned the Dermatology Life Quality Index and Quality of Life Index for Atopic Dermatitis; however, consensus on a preferred instrument for measuring this domain could not be reached. In summary, POEM is recommended as the HOME core outcome instrument for measuring AE symptoms., (© 2016 The Authors. British Journal of Dermatology published by John Wiley & Sons Ltd on behalf of British Association of Dermatologists.)
- Published
- 2016
- Full Text
- View/download PDF
50. Pediatric myositis ossificans mimicking osteosarcoma.
- Author
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Yamaga K, Kobayashi E, Kubota D, Setsu N, Tanaka Y, Minami Y, Tanzawa Y, Nakatani F, Kawai A, and Chuman H
- Subjects
- Biopsy, Child, Diagnosis, Differential, Female, Humans, Magnetic Resonance Imaging, Tomography, X-Ray Computed, Bone Neoplasms diagnosis, Diagnostic Errors, Myositis Ossificans diagnosis, Osteosarcoma diagnosis
- Abstract
Myositis ossificans (MO) is a rare benign cause of heterotopic bone formation in soft tissue that most commonly affects young adults, typically following trauma. We report the case of an 11-year-old girl who developed MO mimicking osteosarcoma in her right shoulder. Plain radiography and computed tomography showed poorly defined flocculated densities in the soft tissue and a periosteal reaction along the proximal humerus. On magnetic resonance imaging, the mass displayed an ill-defined margin and inhomogeneous signal change. Histologically, the mass had a pseudosarcomatous appearance. Based on these findings, the patient was initially misdiagnosed with osteosarcoma at another hospital. The diagnosis was difficult because the patient was 11 years old and had no trauma history, with atypical radiographic changes and a predilection for the site of origin for osteosarcomas. We finally made the correct diagnosis of MO by carefully reviewing and reflecting on the pathological differences between stages., (© 2015 Japan Pediatric Society.)
- Published
- 2015
- Full Text
- View/download PDF
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