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5. Platform for in situ real-time measurement of protein-induced conformational changes of DNA.

Author

Spuhier, Philipp S., Kneževuc, Jelena, Yalçin, Ayca, Bao, Qiuye, Pringsheim, Erika, Dröge, Peter, Rant, Ulrich, and ÜnIü, M. Selim

Subjects
CONFORMATIONAL analysis, ESCHERICHIA coli, PROTEIN binding, DNA microarrays, LITHOGRAPHY, BIOSENSORS
Abstract

A platform for in situ and real-time measurement of proteininduced conformational changes in dsDNA is presented. We combine electrical orientation of surface-bound dsDNA probes with an optical technique to measure the kinetics of DNA conformational changes. The sequence-specific Escherichia co/i integration host factor is utilized to demonstrate protein-induced bending upon binding of integration host factor to dsDNA probes. The effects of probe surface density on binding/bending kinetics are investigated. The platform can accommodate individual spots of microarrayed dsDNA on individually controlled, lithographically designed electrodes, making it amenable for use as a high throughput assay. [ABSTRACT FROM AUTHOR]

Published
2010

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