Search

This work evaluated the potential of Camellia pollen extracts as an antioxidant and lipase activity inhibitor from the pollen composition and the extraction solvent. The lipase inhibitory activity, the scavenging radical's ability (ABTS and DPPH) and the ferric-reducing ability of the seven extracts were examined. The fatty acid profile of the extracts with excellent antioxidant and lipase inhibitory activity was also determined by GC-MS. The ethyl acetate (IC50 = 2.07±0.20 mg/mL) and the acetone extracts (IC50 = 1.19±0.07 mg/mL) showed better lipase inhibitory activity. For the antioxidant activity, the methanol (IC50 = 2.55±0.34 mg/mL), ethanol (IC50 = 2.87±0.23 mg/mL) and the water (IC50 = 2.62±0.05 mg/mL) extracts had superior ABTS radical-scavenging ability. Greater DPPH radical-scavenging ability was seen in the ethanol (IC50 = 2.92±0.04 mg/mL) and acetone extracts (IC50 = 3.09±0.12 mg/mL). The ethyl acetate and petroleum ether extracts manifested better FRAP. The fatty acid composition of the methanol and ethyl acetate extracts belonged to the unsaturated fatty acids. This study indicated that the lipase inhibitory activity and the antioxidant activity found in Camellia pollen may exert dual benefits for preventing and treating obesity. [ABSTRACT FROM AUTHOR]

BACKGROUND Under the situation that the shortage of proteins and large quantity of corn gluten meal ( CGM), which is a superior protein resource, is under-exploited, the enzymatic hydrolysis of CGM was employed to improve its bioavailability because of its special amino acid composition. RESULTS The apparent faecal digestibility and true faecal digestibility of all corn gluten meal hydrolysates ( CGMHs) decreased in varied amounts compared with those of CGM. However, the protein efficiency ratio, the net protein ratio, the biological value, and the net protein utilization of the CGMHs with degree of hydrolysis ( DH) of 4.94% and with DH of 10.06% increased significantly ( P < 0.05). The results of in vitro gastro-intestinal digestion showed that the molecular weight distribution and amino acid composition among different DHs resulted in variances in bioavailability. CONCLUSION Partial hydrolysis of CGM can improve its bioavailability, providing a future protein supplement for protein resources.© 2014 Society of Chemical Industry [ABSTRACT FROM AUTHOR]