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The structure of the title compound, C17H20O4 [systematic name: (1aS,3aR,4aS,5aR)-15-(acetoxy)linden-7(11),8-trieno-12,8-lactone or (4aR,5S,5aR,6aS,6bR)-5-(acetoxymethyl)-4a,5,5a,6,6a,6b-hexahydro-3,6b-dimethylcyclopropa[2,3]indeno[5,6-b]furan-2(4H)-one, ent-chloranthalactone C], a natural product isolated from the whole plant Chloranthus japonicus Sieb., is a typical lindenane-type sesquiterpenoid. The molecule comprises a bicyclo[3.1.0]hexane ring (A/B system) bearing an acetoxymethyl (C-4) group, a bicyclo[4.3.0]nonane ring (B/C system) containing a double bond (C-8/9) and a chiral quaternary carbon (C-10), and a 7(11)-en-12,8-olide structural moiety on the cyclohexan-8-ene (C ring). In the tetracyclic skeleton, the 1,3-cyclopropane ring has a β-configuration, and atoms H-5 and H3-14 have α- and β-orientations, respectively. In the crystal, the molecules are assembled into a two-dimensional network by weak O...H/H...O interactions. Hirshfeld surface analysis illustrates that the greatest contributions are from H...H (55.2%), O...H/H...O (34.6%) and C...H/H...C (8.9%) contacts.

Hepatocellular carcinoma (HCC) is one of the deadliest malignant tumors that are harmful to human health. Increasing evidence has underscored the critical role of the competitive endogenous RNA (ceRNA) regulatory networks among various human cancers. However, the complexity and behavior characteristics of the ceRNA network in HCC were still unclear. In this study, we aimed to clarify a phosphatase and tensin homolog (PTEN)-related ceRNA regulatory network and identify potential prognostic markers associated with HCC. The expression profiles of three RNAs (long non-coding RNAs [lncRNAs], microRNAs [miRNAs], and mRNAs) were extracted from The Cancer Genome Atlas (TCGA) database. The DLEU2L-hsa-miR-100-5p/ hsa-miR-99a-5p-TAOK1 ceRNA network related to the prognosis of HCC was obtained by performing bioinformatics analysis. Importantly, we identified the DLEU2L/TAOK1 axis in the ceRNA by using correlation analysis, and it appeared to become a clinical prognostic model by Cox regression analysis. Furthermore, methylation analyses suggested that the abnormal upregulation of the DLEU2L/TAOK1 axis likely resulted from hypomethylation, and immune infiltration analysis showed that the DLEU2L/TAOK1 axis may have an impact on the changes in the tumor immune microenvironment and the development of HCC. In summary, the current study constructing a ceRNA-based DLEU2L/TAOK1 axis might be a novel important prognostic factor associated with the diagnosis and prognosis of HCC., Graphical Abstract, Shi et al. provide evidence that a ceRNA-based DLEU2L/TAOK1 axis has an impact on changes in the tumor immune microenvironment and might be a novel important prognostic factor associated with the diagnosis and prognosis of HCC.

The problem of low accuracy and low convenience in the existing state of health (SOH) estimation method for vehicle lithium-ion batteries has become one of the important problems in the electric vehicle field. This paper proposes an improved cuckoo search particle filter (ICS-PF) algorithm based on a charging time segment from equal voltage data to estimate battery health status. Appropriate voltage ranges of charging time segments are selected according to the battery charging law, and in the meantime, the charging time segments are collected as a health indicator to establish the corresponding relationship with battery capacity attenuation value. An improved cuckoo search particle filter algorithm based on the traditional particle filter (PF) and cuckoo search (CS) algorithm is proposed by enhancing the search step size and discovery probability to estimate the capacity attenuation. The estimation result shows that this method is superior to the traditional particle filter and cuckoo search particle filter (CS-PF) method, as the maximum estimation error is less than 2%.

Carex agglomerata C. B. Clarke is a sedge with excellent ornamental characters, it is an important ecosystem stabilizer. Here we report the complete chloroplast genome of C. agglomerata to provide a foundation for further phylogenetic studies on the Cyperaceae. The chloroplast (cp) genome is 184,157 bp in size and consists of a large single-copy (LSC) region 106,654 bp in length, a small single-copy (SSC) region of 36,099 bp, two inverted repeats (IR) regions each 20,702 bp. The total GC content of the cp genome is 33.9% with the LSC, SSC, and IR regions 32, 32.5, and 42.9%, respectively. The cp genome contains 128 genes, including 80 protein-coding, 40 tRNA, and eight rRNA genes. The phylogenetic analysis showed C. agglomerata is in a clade with Carex neurocarpa Maxim and Carex siderosticta Hance. This study provides a basis for further phylogenetic studies of Carex.

The complete chloroplast genome of Fargesia qinlingensis (Poaceae) has been reconstructed from the whole-genome Illumina sequencing data. The complete chloroplast genome sequence of F. qinlingensis obtained in this study was 139,640 bp in length, with a large single copy (LSC) region of 83,220 bp, a small single copy (SSC) region of 12,826 bp, separated by two inverted repeat (IR) regions of 21,797 bp each. The GC content was 38.9%, and in the LSC, SSC, and IR regions were 37.0%, 33.2%, and 44.2%, respectively. A total of 130 genes were annotated, including 83 protein-coding genes (PCGs), 39 transfer RNA (tRNA) genes, and eight ribosomal RNA (rRNA) genes. The result of the phylogenetic analysis showed that F. qinlingensis was more closely related to the species of F. denudata.

Xiphidiopsis (Xiphidiopsis) gurneyi belongs to Meconematinae. The complete mitochondrial genome of X. (X.) gurneyi was sequenced by the next-generation sequencing (NGS) technology. The total length of the mitogenome was 16,225 bp and contains the typical gene arrangement, base composition, codon usage found in Meconematinae species. Phylogenetic tree was constructed based on concatenated datasets of PCGs and rRNAs of X. (X.) gurneyi and 19 Tettigoniidae species to assess their phylogenic relationship. Phylogenetic analysis showed that X. (X.) gurneyi was more closely related to the genus of Xizicus.

The length of Alloxiphidiopsis emarginata complete mitogenome was 16,207 bp and contained the typical gene arrangement, base composition, codon usage found in other related species. The overall base composition exhibited obvious anti-G (10.6%) and AT bias (71.6%). The initiation codons of all PCGs were typical ATN (ATA/ATG/ATT), and the termination codons were TAA, TAG, or incomplete stop codon T. All tRNAs could be folded into typical cloverleaf secondary structures, except tRNASer (AGN). Phylogenetic analyses showed that A. emarginata was closer with Xizicus howardi.

The performance of hybrid electric vehicle motor directly affects its power performance and fuel economy. The high performance sintered NdFeB permanent magnet material is chosen as the core material of the hybrid vehicle motor for the design of the permanent magnet in this paper, and its size is also determined. A doubly salient permanent magnet motor is proposed here on the basis of the selected NdFeB permanent magnet material, and its structure size is designed. The finite element numerical calculation is carried out for the motor in particular rotor position angle on the basis of the field quantity to obtain the magnetic field distribution law in several typical rotor positions and the static characterise parameters of the motor are further obtained. Final, inspect the performance of the prototype objectively based on the bench experiment of the designed physical prototype, and the prototype experiment and finite element results both proved that the designed doubly salient permanent magnet moto...

Some species of Allium in Liliaceae have fistular leaves. The fistular lamina of Allium fistulosum undergoes a process from solid to hollow during development. The aims were to reveal the process of fistular leaf formation involved in programmed cell death (PCD) and to compare the cytological events in the execution of cell death to those in the unusual leaf perforations or plant aerenchyma formation. In this study, light and transmission electron microscopy were used to characterize the development of fistular leaves and cytological events. Terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assays and gel electrophoresis were used to determine nuclear DNA cleavage during the PCD. The cavity arises in the leaf blade by degradation of specialized cells, the designated pre-cavity cells, in the center of the leaves. Nuclei of cells within the pre-cavity site become TUNEL-positive, indicating that DNA cleavage is an early event. Gel electrophoresis revealed that DNA internucleosomal cleavage occurred resulting in a characteristic DNA ladder. Ultrastructural analysis of cells at the different stages showed disrupted vacuoles, misshapen nuclei with condensed chromatin, degraded cytoplasm and organelles and emergence of secondary vacuoles. The cell walls degraded last, and residue of degraded cell walls aggregated together. These results revealed that PCD plays a critical role in the development of A. fistulosum fistular leaves. The continuous cavity in A. fistulosum leaves resemble the aerenchyma in the pith of some gramineous plants to improve gas exchange.

The formation of secretory cavities in Rutaceae has been the subject of great interest. In this study, cytological events that are involved in the lysigenous formation of the secretory cavities in the leaves of Dictamnus dasycarpus are characterized by an interesting pattern of programmed cell death (PCD). During the developmental process, clusters of cells from a single protoepidermal cell embark on different trajectories and undergo different cell death fates: the cell walls of the secretory cells have characteristics of thinning or complete breakdown, while the sheath cells present a predominantly thick-walled feature. A DAPI assay shows deformed nuclei that are further confirmed to be TUNEL-positive. Gel electrophoresis indicates that DNA cleavage is random and does not result in ladder-like DNA fragmentation. Ultrastructurally, several remarkable features of PCD have been determined, such as misshapen nuclei with condensed chromatin and a significantly diffused membrane, degenerated mitochondria and plastids with disturbed membrane systems, multivesicular bodies, plastolysomes, vacuole disruption and lysis of the center secretory cell. Cytological evidence and Nile red stains exhibit abundant essential oils accumulated in degenerated outer secretory cells after the dissolution of the center secretory cell. In addition, explanations of taxonomic importance and the relationship between PCD and oil droplet accumulation in the secretory cavities are also discussed.

Chloroplast (cp) genome sequences became a widely used tool for evolutionary and phylogenetic studies in plants. Abies chensiensis (Pinaceae: Abietoideae) is an endangered species endemic to China. To understand its evolutionary characteristics, the complete chloroplast genome of the A. chensiensis has been reconstructed from the whole-genome Illumina sequencing data. The circular genome is 121,784 bp in length and without a typical quadripartite structure due to the loss of IR region. The total GC content of whole genome sequence is 38.3%. The chloroplast genome encodes 109 genes, including 75 protein-coding genes, 30 transfer RNA genes and four ribosomal RNA genes. Among them, 35 genes involved in photosynthesis, while 58 genes involved in self-replication. The Maximum-Likelihood phylogenetic analysis showed a strong sister relationship with A. nephrolepis and A. koreana in Abietoideae. Our findings provide fundamental information for further evolutionary and phylogenetic researches of Abietoideae.

The complete chloroplast genome sequence of Echinocodon lobophyllus was determined by Illumina pair-end sequencing. The results showed that the complete plastid genome was 169,419 bp in length, containing a large single copy (LSC) of 85,599 bp and a small single copy (SSC) of 8054 bp, which were separated by a pair of 37,883 bp inverted repeats (IRs). A total of 109 unique genes were annotated, including 75 protein coding genes, 30 tRNA genes, and 4 rRNA genes. Among these genes, 16 genes contained one or two introns. The overall GC contents of the plastid genome were 38.4%. A maximum likelihood phylogenetic analysis showed that E. lobophyllus and Codonopsis minima formed one clade at the base of the phylogenetic tree of Campanulaceae with a high support value.

The ontogeny of a special type of glandular hairs, namely the trichome-like cavities in Dictamnus dasycarpus , characterized by many morphological similarities to non-glandular hairs, capitate glandular hairs, and secretory cavities, was studied using light and electron microscopy. These trichome-like cavities originate from a single, initial epidermal cell that undergoes a periclinal division, with one cell developing into the internal cells and the other into the outer, epidermal cells. A beak-shaped apex is formed on the head of the trichome-like cavity. The histochemical test shows that the trichome-like cavities are important sites for lipid production. By ultrastructural analysis it becomes evident that formation of these trichome-like cavities starts with a disorganization of the cytoplasm that is accompanied by formation of odd shaped nuclei with condensed chromatin. The process continues bringing about plasmolytic processes, and disintegration of the plasma membrane system follows leading finally to autolysis, when mitochondria and degenerated plastids with disorganized membrane systems are engulfed by vacuoles, multivesicular bodies, and double-membranous autophagosomes within the vacuoles. A strong structural twist and swelling of the internal cell walls ultimately leads to complete breakdown of the structures. Nuclei of the inner internal cells within the trichome-like cavity become TUNEL-positive and DAPI-negative first; later this is detected also in the outer internal cells, indicating a centrifugal nuclear degradation process. On the basis of this work, it can be assumed that the lysigenous formation of the trichome-like cavity is a typical programmed cell death process.

It is important to detect the vehicle rear axle on-line during production process. To realize the quality detection of the rear axle, vibration diagnosis method is adopted to analyze the vibration characteristics of the rear axle and calculate characteristic frequency. The on-line detection control system of vehicle rear axle quality is designed by extracting characteristic parameters of correctly reflecting rear axle running state and applying PLC system. The system has been operated stably, reliably and efficiently through the test. It has a practical significance.

This paper presents a low-cost, simple and reliable solution for automotive electronics Hardware-In-Loop (HIL) simulation, taking Jetta AT1.6 car as object, designs and develops automotive electronics HIL simulation platform. Firstly, the overall structure of the platform is designed; secondly, system hardware platform is developed and built using Protel DXP; then, HIL simulation models of ABS (Anti-lock Braking System), engine and automatic transmission are built using Matlab/Simulink and develop automotive electronics HIL simulation platform; finally, carry on the experiment and the results show that the designed HIL simulation experimental platform is good. The platform has widespread applicability in the teaching experiments and the aspects of developing, debugging and testing of automotive electronics control systems.

Programmed cell death (PCD), a topic of abiding interest, remodels plants at the cell, tissue, and organ levels involving various developmental processes of plants. The aim of this study is to provide a morphological characterization of evidence of PCD involvement in the laticiferous canal formation in fruit of Decaisnea fargesii. Several ultrastructural features of PCD have been observed including disintegration of vacuole and plasma membranes, cell wall degeneration, degenerated cytoplasm, abundant membrane structures and flocculent material, mitochondria and misshapen nuclei coupled with degraded plastids in vacuoles, and nuclei enveloped by rubber granule. In D. fargesii, the nuclei of the secretory epidermal cells become TUNEL-positive from the sunken stage to the late expanding stage, then DAPI-negative during the mature stage, indicating an early event of deoxyribonucleic acid (DNA) cleavage and a late event of complete DNA degeneration. Gel electrophoresis indicates that DNA cleavage is random and does not result in the laddering pattern indicating multiples of internucleosomal units. During the PCD of secretory epidermal cells, the rubber granules continue to be synthesized and accumulated in the secretory epidermal cells despite nuclear degradation. The PCD’s role in laticiferous canal formation suggests that PCD may play important roles in gland development of plants.

The development of secretory canals in vegetative organs of Bupleurum chinense DC. and the accumulation of essential oils were investigated by transmission electron microscopy (TEM) analysis. The secretion mechanism of the essential oil was also discussed. The results indicate that the plastid, ground substances of cytoplasm and mitochondria took part in the biosynthesis of oil or oil precursor. The endoplasmic reticulum involved in the transport of essential oil to the secretory lumen. At latter stages of development of secretory cells, numerous different sized vesicles fused with the plasmalemma along the boundary between two neighbor secretory cells and secreted the substance into the wall. Thus, the wall between two neighbor secretory cells near the lumen became loosely structured. Then, the wall lined the lumen near two neighbor secretory cells extruded numerous grey vesicles with various sizes on the side facing the lumen, and released these vesicles into the lumen. As a result, the manner of secretion in secretory canals of Bupleurum chinense DC. appeared to be exocytosis.