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Ziziphi Spinosae Semen (ZSS) is a valued seed renowned for its sedative and sleep-enhancing properties. However, the price increase has been accompanied by adulteration. In this study, chromaticity analysis and Fourier transform near-infrared (FT-NIR) combined with multivariate algorithms were employed to identify the adulteration and quantitatively predict the adulteration ratio. The findings suggested that the utilization of chromaticity extractor was insufficient for identification of adulteration ratio. The raw spectrum of ZMS and HAS adulterants extracted by FT-NIR was processed by SNV + CARS and 1d + SG + ICO respectively, the average accuracy of machine learning classification model was improved from 77.06 % to 97.58 %. Furthermore, the R2 values of the calibration and prediction set of the two quantitative prediction regression models of adulteration ratio are greater than 0.99, demonstrating excellent linearity and predictive accuracy. Overall, this study demonstrated that FT-NIR combined with multivariate algorithms provided a significant approach to addressing the growing issue of ZSS adulteration.

AIM: To explore the expression levels of long non-coding RNA maternally expressed gene 3(lncRNA MEG3)and Micro RNA-138(miR-138)in serum of patients with wet age-related macular degeneration(ARMD)hemorrhage and their relationship with the prognosis of patients.METHODS: A prospective study. A total of 90 patients with wet ARMD hemorrhage admitted from January 2018 to June 2021 were selected as the observation group, and 78 people who underwent physical examination in our hospital during the same period were selected as the control group. Real-time fluorescent quantitative PCR(qRT-PCR)was used to detect the expression levels of serum lncRNA MEG3 and miR-138 in all subjects. Patients in the observation group were injected with Ranibizumab for treatment once a month, for a total of 3 times. The observation group was followed up for 3mo after treatment, and the patients were divided into good prognosis and poor prognosis group.The levels of lncRNA MEG3 and miR-138 were compared between the two groups. Pearson correlation was used to analyze the correlation between lncRNA MEG3 and miR-138. The receiver operating characteristic curve(ROC)was used to analyze the value of judging the poor prognosis of patients with wet ARMD hemorrhage. Multivariate Logistic regression was used to analyze the factors affecting the poor prognosis of patients with wet ARMD hemorrhage.RESULTS: Compared with the control group, the level of serum lncRNA MEG3 in the observation group decreased(1.13±0.37 vs 0.71±0.21), and the miR-138 level increased(1.05±0.29 vs 2.23±0.54; all P

AIM: To investigate the effects and safety of intravitreal injection of Conbercept combined with macular grid pattern photocoagulation for macular edema secondary to non-ischemic branch retinal vein occlusion(BRVO). METHODS: A total of 38 patients(38 eyes )with macular edema following non-ischemic BRVO were retrospectively analyzed. All patients were given best corrected visual acuity(BCVA), intraocular pressure, slit lamp with preset lens, optic coherent tomography(OCT)and fluorescence fundus angiography(FFA)examination. The BCVA and central macular thickness(CMT)were observed before and 1wk, 1, 2, 3, 6, 9 and 12mo after treatment. The change in pre-treatment and post-treatment were compared, and the related complications were recorded. RESULTS:BCVA before treatment and 1wk, 1, 2, 3, 6, 9 and 12mo after treatment were 0.89±0.62, 0.64±0.59, 0.55±0.62, 0.46±0.43, 0.53±0.67, 0.43±0.38, 0.42±0.29, 0.40±0.30, the difference was statistically significant between that before and after treatment(PP>0.05). CMT were 683.25±236.47, 298.65±116.12, 276.89±107.28, 281.55±99.62, 251.41±119.47, 240.58±88.62, 231.74±75.36, 209.51±84.68μm, the difference was statistically significant between that before and after treatment(PP>0.05). There were 11 eyes received one injection, 18 eyes with two injections, 2 eyes with 3, 3 eyes with 4, and 4 eyes more than 4, the average injection was 2.01±1.42. The CMT decreased after every injection. The intraocular hypertension and other complications were not seen after treatment. CONCLUSION: Intravitreal injection of conbercept combined with macular grid pattern photocoagulation for macular edema to non-ischemic BRVO can reduce the macular edema and improve BCVA, which is effective and safety.