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Nucleus-encoded circular RNAs (ncircRNAs) have been widely detected in eukaryotes, and most circRNA identification algorithms are designed to identify them. However, using these algorithms, few mitochondrion-encoded circRNAs (mcircRNAs) have been identified in plants, and the role of plant mcircRNAs has not yet been addressed. Here, we developed a circRNA identification algorithm, mitochondrion-encoded circRNA identifier, based on common features of plant mitochondrial genomes. We identified 7,524, 9,819, 1,699, 1,821, 1,809, and 5,133 mcircRNAs in maize (Zea mays), Arabidopsis (Arabidopsis thaliana), rice (Oryza sativa), tomato (Solanum lycopersicum), cucumber (Cucumis sativus), and grape (Vitis vinifera), respectively. These mcircRNAs were experimentally validated. Plant mcircRNAs had distinct characteristics from ncircRNAs, and they were more likely to be derived from RNA degradation but not intron backsplicing. Alternative circularization was prevalent in plant mitochondria, and most parental genomic regions hosted multiple mcircRNA isoforms, which have homogenous 5′ termini but heterogeneous 3′ ends. By analysis of mitopolysome and mitoribosome profiling data, 1,463 mcircRNAs bound to ribosomes were detected in maize and Arabidopsis. Further analysis of mass spectrometry-based proteomics data identified 358 mcircRNA-derived polypeptides. Overall, we developed a computational pipeline that efficiently identifies plant mcircRNAs, and we demonstrated mcircRNAs are widespread and translated in plants.

The effects of rainfall intensity on stemflow production as described in the literature are quite inconsistent (increase, decrease, or no clear relationship) due to complex interactions between rai...

Dialysis patients with erythropoietin hypo-responsiveness suffered from refractory anemia. Roxadustat reversibly binds and inhibits hypoxia-inducible factor-prolyl hydroxylase (HIF-PHD), resulting in increased endogenous EPO which stimulates erythropoiesis, theoretically has an advantage over exogenous EPO in anti-anemia therapy. From September 2019 to October 2020, 32 dialysis patients with hypo-responsiveness to erythropoietin were evaluated. During the 24-week follow-up period, all patients were taken off erythropoietin and switched to roxadustat. Dosage adjustments were administrated according to the fluctuation of hemoglobin level during the treatment. Parameters about anemia, iron metabolism and biochemical indexes were collected, and adverse events were recorded. A total of 31 patients completed the clinical observation, with varying degrees of malnutrition-inflammation. Post treatment, the levels of transferrin and total iron-binding capacity were increased, while that of transferrin saturation and cholesterol decreased. 15 cases (accounting for 48.39%, designated as fulfilled group) met the target level of hemoglobin, while 16 cases (51.61%, non-fulfilled group) did not. The baseline conditions of the above two groups were compared. The levels of hypersensitive C-reactive protein, interleukin-6 and serum ferritin in the non-fulfilled group were higher than those in the fulfilled group, and the levels of residual renal function, serum albumin, iron, transferrin and total iron-binding capacity were lower than those in the fulfilled group. Linear regression analysis showed that increase of HsCRP had a negative effect on the improvement of Hb. One case of adverse reaction grade 3 and four cases of grade 2 occurred throughout the study, yet all were relieved after therapy. Significant anti-anemia effects could be achieved in most patients with erythropoietin hypo-responsiveness after treatment with roxadustat, accompanied by relatively mild and rare adverse reactions. The malnutrition-inflammation states of patients may interfere with the anti-anemia effect of roxadustat, and iron utilization is more important than iron storage in anemia improvement.

Cultural creative industries (CCIs) have become an important driver in motivating the modern economy around the world, and the sustainable development of CCI is calling for a proper profit mechanism. Using China as the research context, this article investigates how copyright is used in the development of CCIs. The cities of Shenzhen and Hangzhou are selected as cases, and I identify 98 representative CCI enterprises from Shenzhen and 127 representative CCI enterprises from Hangzhou to conduct the analysis. It is found that the development of CCIs in different cities shows different models with regional characteristics, and shares some characteristics in common; most CCI enterprises have experience with copyright registration; copyright is highly correlated with other types of intellectual property (e.g., patent, trademark), and there is an obvious integration of copyright and technology; judicial lawsuits have become a major tool for enterprises to use copyright to protect their benefits, with plaintiffs winning the majority of the lawsuits. This research has both significant theoretical and practical implications, and contributes to theory about the use of copyright in the development of CCI at a regional level.

The influence and its mechanism of ultrasound on acetaldehyde/glyoxylic acid competing bridged the polymerization coloration of flavan-3-ols in model wine solution were investigated by high-performance liquid chromatography-mass spectrometry (HPLC-MS) and kinetics and thermodynamic model. The results indicate that ultrasound could significantly accelerate the polymerization coloration and further modify wine color. In addition, the polymerization reaction conformed first-order reaction model, and the reaction rate constant (k) values were markedly increased by ultrasound, accelerating the coloration reaction, especially in the model wine containing glyoxylic acid. Besides, the polymerization processing was non-spontaneous and endothermic according to the thermodynamic analysis. In conclusion, ultrasound was indeed conducive to accelerate glyoxylic acid/acetaldehyde-bridged the polymerization of flavan-3-ols and further effect the wine color, which could provide a theoretical basis for the scientific analysis of the mechanism of ultrasound modifying wine color.

To solve the fatigue cracking problem in orthotropic steel decks (OSD) and to prevent the early damage of bridge pavements resulting from the low stiffness of the bridge deck system, epoxy-resin-perfused concrete (RPC) pavements were developed for improving the modulus and road performance of paving materials. The mechanical and road performance of RPC and the bonding performance of the waterproofing adhesive layer were studied via laboratory experiments. The mechanical property of RPC pavements and their protective function on OSD were analyzed by using a finite element model. Composite beam fatigue life and acceleration loading tests were performed on a scaled model to verify the performance of these pavements. Results show that RPC has excellent high-and low-temperature stability and water stability that can meet the requirements and has a relatively long fatigue life. The bonding strength of RPC pavements and steel plate is better than that of epoxy asphalt concrete and Gussasphalt concrete pavements. RPC pavements have a relatively low tensile stress on the surface and a relatively high shear stress below the surface, but these stresses are far lower than the material strength. When paved with RPC, the stress in the fatigue details of OSD is reduced by more than 20%. The maximum relative deflection between ribs, the minimum curvature radius, and the relative deflection to span ratio of RPC are also favorable, thereby providing additional protection for the bridge deck system. The fatigue life tested with a five-point bending composite beam is more than 1 million times, which meets the requirements. In the acceleration loading test, RPC pavements do not show any rut, whereas the OSD shows no cracking when loaded for 12 thousands times. The engineering application results demonstrate the feasibility of using RPC pavements and show that these pavements and OSD endure no damage.

Isotopic composition of gross rainfall has been extensively used as a conservative tracer to track water movement and other hydrological processes in vegetated ecosystems. Recent studies from forest ecosystems, however, demonstrated that vegetation canopies can alter the isotopic composition of rainwater during rainfall partitioning into throughfall and stemflow, likely leading to errors and biases in aforementioned studies. No known studies, to date, had investigated this topic in shrub-dominated arid and semi-arid ecosystems where water is typically the driving factor in ecological, hydrological and biogeochemical processes. In this study, event-based gross rainfall, the throughfall and stemflow induced by shrubs of Caragana korshinskii were measured and samples were collected within a water-limited arid desert ecosystem of northern China, and their water stable isotopes (18O and 2H) were also analyzed in the laboratory. We mainly aimed to investigate whether there is an isotopic enrichment or depletion in stemflow and throughfall in comparison to gross rainfall, and to evaluate the possible underlying mechanisms. Our results indicated an enrichment of both isotopes in stemflow, while a general more depletion in throughfall than in gross rainfall, which is presumably affected by a combinative effects of canopy evaporation, isotopic exchange, and selective canopy storage. Deuterium excess of stemflow were found to be significantly higher (P

The kinetics and mechanisms of the degradation of sulfamethoxypyridazine (SMP) by an ultraviolet-activated persulfate (UV/PS) advanced oxidation process (AOP) and the formation of disinfection byproducts (DBPs) during subsequent chlorination were investigated in this study. The UV/PS process can significantly remove SMP through pseudo-first-order reaction kinetics. In a competitive kinetic experiment, the second-order rate constant of SMP with sulfate radical was determined to be 2.73 × 1010 M−1 s−1, while that with hydroxyl radical was 2.22 × 1010 M−1 s−1. Six major transformation products in SMP degradation were recognized by LC/MS/MS analysis. It was assumed that the degradation pathway of SMP involves the hydroxylation of the aromatic ring, cleavage of the sulfonamide bond, oxidation of the aniline moiety and elimination of SO2. The impacts of persulfate dose, pH, anions (HCO3–, SO42−, and Br−) and humic acid (HA) concentration on SMP degradation efficiency and DBP formation during subsequent chlorination were also examined. SMP degradation was accelerated with increasing persulfate dose, HCO3– and Br- concentration as well as decreasing pH and HA concentration. However, the amount of chloroform (CF) formed was reduced under a higher persulfate dosage and lower pH. In contrast, the amount of dichloroacetonitrile (DCAN) formed was enhanced under a higher persulfate dose and lower pH. Adding HA also increased the formation of both CF and DCAN. While, both HCO3– and SO42– had little effects on the formation of CF and DCAN. The presence of Br– had no significant effects on the bromine incorporation factors (BIFs) of trihalomethanes (THMs) and dihaloacetonitriles (DHANs). Raw water experiments showed that the UV/PS process could destroy SMP in natural water as well as control the formation of DBPs.

Background: Percutaneous coronary intervention (PCI) has been an effective treatment for acute coronary syndrome (ACS) patients. Acute kidney injury (AKI) is one of the common complications after PCI, which seriously affects the living quality and survival time of patients. The approach followed for the patient with AKI after PCI depends on the clinical context and may vary by resource availability. Summary: This review focuses on the pathophysiologies, influencing factors, and preventive measures of AKI in patients with ACS after PCI. The knowledge may better serve the patients and improve their outcomes. Key Messages: Many studies have been carried out for the definition and standard of AKI in the past few years. Etiologies of AKI after PCI included renal damage of contrast medium and atherosclerotic embolism, cardiac insufficiency and surgical factors on renal function. Basic conditions, treatment modalities, and perioperative changes are major risk factors of AKI. Studies have reported that the prevention of contrast-induced nephropathy, modulating the volume overload, some pharmaceuticals and blood purification treatment are helpful to prevent the occurrence of AKI.

The most common odor substance found in surface water is 2-methylisoborneol (2-MIB), and this substance cannot be effectively removed by conventional water treatment processes. Persulfate (S2O82−, PS)-based oxidation has recently received wide attention due to its promising ability to remove refractory pollutants. In this research, the degradation of 2-MIB by PS activated with iron (II) (PS-Fe2+) or heat (PS-Heat) was investigated. Kinetic analysis indicated that 2-MIB degradation in both of the systems (PS-Heat and PS-Fe2+) followed a pseudo-first-order reaction. For the PS-Heat system, a high temperature, a high PS initial concentration and weakly acidic conditions benefit the degradation of 2-MIB. Coexisting anions inhibit 2-MIB removal in the following order: CO32− > HCO3− > Cl−. For the PS-Fe2+ system, the optimum molar ratio of PS to Fe2+ ([PS]: [Fe2+]) was 1:1. The 2-MIB degradation rate increased under acidic conditions and with the addition of citrate; however, the rate decreased with an excess Fe2+ dosage. In addition, this study was the first to examine the behavior pattern of algal photosynthesis activity as well as the total and extracellular 2-MIB of Pseudoanabaena sp. (a typical 2-MIB-producing cyanobacterium) in the PS-Fe2+ system. Moreover, a comparison study of the removal of 2-MIB in actual algae-containing water under the oxidation of PS-Fe2+, potassium permanganate (KMnO4), and sodium hypochlorite (NaClO) was firstly carried out. The results provide useful theoretical and engineering information for treating algae-loaded water.

Osteoporosis is a prevalent systemic skeletal disorder entailing bone fragility and increased fracture risk, often emerging in post-menopausal life. Emerging evidence implicates the dysregulation of microRNAs (miRNAs or miRs) in the progression of osteoporosis. This study investigated the effect of miR-199a-3p on osteoporosis and its underlying mechanism. We first examplished an ovariectomized (OVX)-induced rat osteoporosis model, and then isolated mesenchymal stem cells (MSCs) from bone marrow of the model rats. The overexpression and knock down of miR-199a-3p were conducted in OVX rats and MSCs to verify the role of miR-199a-3p on MSC differentiation. Calcium nodules were measured using alizarin red S (ARS) staining. RT-qPCR and Western blot assay were performed to measure the expression of miR-199a-3p, Kdm3a and osteogenic differentiation-related markers in rat tissues and cells. The correlation between miR-199a-3p and Kdm3a was confirmed using dual-luciferase reporter assay. The enrichment of Kdm3a at the Erk2 and Klf2 promoter was assessed using chromatin immunoprecipitation (ChIP) assay. Isolated MSCs were positive for CD29, CD44, CD90, and CD45, suggesting successful isolation of MSCs. There was increased expression of miR-199a-3p and inhibited osteogenic differentiation in OVX rats. Kdm3a was negatively targeted by miR-199a-3p. Our results also demonstrated that Kdm3a elevated the expression of Erk2 and Erk2 by promoting Erk2 and Klf2 demethylation, which further contributed to osteogenic differentiation. Overall, our results revealed a regulatory network of miR-199a-3p in osteogenic differentiation, highlighting miR-199a-3p as a potential target for therapeutic interventions in osteoporosis.

Background Ti-Ni shape-memory patella concentrator (TNSMPC) has been designed as an alternative approach for fixation of patella fracture, which has some advantages like higher hardness, higher tenacity, better wearing resistance, excellent corrosion resistance and desired histocompatibility. The present study was to investigate the efficiency of TNSMPC combined with cannulated compression screws in the treatment of comminuted patella fractures. Methods Between January 2014 and December 2017, 54 patients of C2 and C3 patella fractures underwent open reduction and internal fixation with TNSMPC combined with cannulated compression screws. All the patients got standard postoperative rehabilitation programs and were regularly followed up for at least 12 months after the operation. X-rays, knee functions and life quality were evaluated during the follow-up. Results All the patients achieved bone healing and recovery of knee function with low incidence of complications according to outcomes of X-rays and questionnaires. The average operation time and blood loss during surgery were 77.5 ± 25.12 min and 24.25 ± 4.70 ml respectively. The Knee Outcome Survey Activities of Daily Living Scale, the range of motion and the 36-item short-form heath survey after the operation were all improved. According to the Bostman’s criteria, the excellent to good rate was 92.6%. Conclusion TNSMPC combined with cannulated compression screws is an effective internal fixation method for C2 and C3 patella fracture with excellent clinical outcomes. In addition, the operation does not increase extra technique difficulty or tissue damage relatively, which is worth promotion.

Understanding the actual evapotranspiration (ET) variation of the sparsely distributed xerophytic shrubs is crucial to accurately upscale community ET to ecosystem scale. Here we quantified the actual ET of two dominant xerophytic shrubs of the Tengger Desert in northwestern China, i.e. Salsola passerina and Reaumuria soongorica, by using four large weighing lysimeters. The results showed that with the increase in precipitation from 140 to 171 mm in the year 2015/2016, the daily mean evaporation (E) of the bare area, and ET of the single shrub communities of S. passerina, R. soongorica, and the associated shrub community (S. passerina + R. soongorica) increased 50, 60, 44, and 47%, respectively; correspondingly, the total E and ET increased 49, 61, 44, and 47%, respectively. The variation of soil moisture within 0–40 cm depth plays a vital role in regulating the E and ET. The new shoot length, as one of important parameters of the xerophytic shrub, was significantly exponentially related to the cumulative ET. From the long- and short-term perspective, event-based precipitation and wind speed are the dominant driving factors behind changes in E and ET, respectively. Relative humidity is the main influencing factor for E and ET after a large rainfall event within 8 days.

Numerous field experiments had demonstrated great spatial variability and temporal stability of throughfall under tree canopies within forested ecosystems. Nonetheless, no known studies have investigated the intrastorm spatial-temporal variability of throughfall beneath xerophytic shrub canopies within arid desert ecosystems where water is typically the principal limiting factor determining the structure and dynamics of ecosystems. Here we investigated the spatial-temporal pattern of throughfall at intrastorm scale, and systematically examined the effects of meteorological variables on throughfall based on the principal components analysis (PCA) and a multiple regression model. Throughfall was monitored at 10-min intervals by placing tipping-bucket rain gauges at different radial directions beneath 3 shrubs of Caragana korshinskii during the growing season of 2016 within a water-limited arid desert ecosystem of northern China. We found the temporal heterogeneity of rainfall clearly affected the timing of throughfall beneath shrub canopies within discrete rainfall events. Throughfall also differed markedly among different radial directions beneath shrub canopies, which was found to be well associated with wind directions during rainfall events. PCA on meteorological variables indicated that three principal components accounted for 84.2% of the total variance, and we found that the second principal component (loaded strongly on rainfall amount and maximum 10-min rainfall intensity) was the dominant component controlling throughfall and its spatial variability after introducing three principal components into a multiple linear regression model. Our findings highlight the spatial-temporal variability of throughfall at the intrastorm scale, and are expected to be helpful for an improved process-based characterization and modelling of throughfall in vast arid desert ecosystems.

A new proton therapy facility (HUST-PTF) is under construction in the Huazhong University of Science and Technology. The integral field measurement system of dipole magnets was designed for the beamline of HUST-PTF. The design goal is to build a reliable, precise, and automatic measurement system, which is accurate enough to characterize the magnetic field quality of the dipole magnets. The system consists of long coils, precision motion stages, data acquisition, and control system. The design of the whole system and the measurement method is described in this paper. The design and fabrication of the long coil are also discussed, and the errors from the geometric frame of coil and electronic equipment are analyzed.

The WC particles reinforced iron matrix composites were prepared by utilizing energy ball milling powder mixed and vacuum powder sintering method in this paper. The effects of two kinds of matrix on the micro-structure, interface and fracture mechanism of the composites were studied emphatically, and it provided a theoretical guidance for the design and engineering application of particle reinforced metal matrix composites. The results show that: in the two kinds of matrix composites, WC particles and interface had different degree of melting, WC particles and the matrix were metallurgical combination; ferritic matrix composites had better compressibility than pearlite matrix composites (1089Mpa); the fracture mode of ferrite matrix composites was quasi-cleavage fracture and pearlite matrix composites was pure cleavage fracture; the compressive micro-cracks of the two matrix composites generated at the interface and expand at the interface to a broad macroscopic crack, which eventually the material fails.

Stemflow has long been recognized as an important ecohydrological process in drylands, and may influence dynamics of woody plants by altering rainfall redistribution. However, quantification of the effects of stemflow on woody plants dynamics remains largely unclear. It is also unknown whether the effects of stemflow are consistent for individual plants and ecosystems where woody plants grow. This study developed an ecohydrological model to explore how stemflow affected individual woody plants and ecosystems, and how the effects changed along a rainfall gradient (10–600 mm) in a dryland ecosystem. This study demonstrated the positive effects of stemflow on woody plants in both individual (by a degree of 16–48%) and ecosystem scales (by a degree of 8–45%). Stemflow started to promote woody plant growth in the individual scale even when annual rainfall was very low (

Xanthylium cations and its ethyl ester are important pigments derived from (+)-catechin and glyoxylic acid in wine. In this paper, ultrasound treatment was applied in model wine containing glutathione to study its effects on the formation of xanthylium cation pigments triggered by free radicals from the ultrasound cavitation. The changes in color parameters, concentration of pigment compounds and free radicals were investigated during wine storage by the means of ultraviolet visible (UV–vis) spectrophotometer, colorimeter, high performance liquid chromatography with mass spectrometry (HPLC-MS) and electron paramagnetic resonance (EPR), respectively. Results indicate that longer ultrasonic treatment in conjunction with glutathione can significantly enhance the formation of xanthylium cation pigments and accelerate the red wine color evolution, and no extra (+)-catechin was involved, which might be attributed to the stronger 1-hydroxyethyl radicals intensity induced by ultrasound irradiation. In summary, ultrasound treatment can accelerate the oxidative coloration of wine with GSH during ageing, which would contribute to well understand the changing mechanism of wine color properties modified by ultrasound.

In this study, we determined the relationship between stemflow amount and morphological metrics of plants. Previous studies on this issue generally neglected the influence of differential rainfall amounts on stemflow, which somehow bias the relationship between stemflow amount and morphological metrics. Here, field observation of stemflow in two xerophytic shrubs ( Caragana korshinskii and Artemisia ordosica ) of varying sizes were conducted during three growing seasons in 2011–2013 to evaluate this relationship by using a simple variable, i.e., stemflow volume per unit rainfall (SfVPR, mL mm −1 ). This stemflow index directly defines the influence of rainfall depth on the morphological metrics of plants, and it has the following advantages over the conventional stemflow coefficient that has been used to determine the influence of tree/shrub morphological metrics on the stemflow amount: (1) it avoids the bias caused by the influences of differential rainfall amount on stemflow yield and (2) it is suitable for use in hydrological models. Our results showed that SfVPR can well be used to determine the relationship between stemflow amount and shrub morphological metrics. Moreover, by using multiple regression model, we found that projected canopy area (or canopy volume, or basal area), plant area index, and stem diameter are the most influential factors for the stemflow amount of C. korshinskii, whereas no significant explanatory variables were found for that of A. ordosica .

Stemflow is known as a highly localized point input of rainwater and solutes around tree/shrub bases where roots are concentrated, thus having considerable effects on hydrology and biogeochemistry of vegetated ecosystems. Stemflow shows a pronounced inter-specific variation due to morphological differences among species, while the intra-specific variation of stemflow has been poorly explored. We systematically examined the effects of shrub morphological metrics on intra-specific funnelling efficiencies by quantifying the stemflow of nine shrubs of Caragana korshinskii within a water-limited arid desert ecosystem of northern China. Stemflow volume was used to compare the absolute amount of stemflow generated by shrubs of varying size, and funnelling ratio was used to assess their funnelling efficiencies. Both rainfall depth and shrub morphological metrics significantly affected stemflow volume, while funnelling ratio was more associated with shrub morphology. Under the same rainfall condition, smaller shrubs produced lower volumes of stemflow, while gaining access to rainfall via higher funnelling ratio than larger shrubs. Our findings highlight a large variation in funnelling efficiency among individual shrubs within the same species, and in particular, smaller shrubs might profit more from sporadic small rainfall events than larger shrubs.

Soil nitrogen (N) mineralization is a microbially-mediated biogeochemical process that is strongly influenced by changing climates. However, little information is available on the mechanisms behind the response of N mineralization to prolonged warming coupled with drought in soils covered by biocrusts. We used open top chambers to investigate the rate of soil N transformation (ammonification, nitrification and mineralization), enzyme activity and gene abundance in response to warming coupled with reduced precipitation over three years (2016-2018). Warming and drought significantly reduced the N transformation rate, extracellular enzyme activity, and gene abundance in moss-covered soil. For cyanobacteria-covered soil, however, it inhibited enzyme activity and increased the abundance of the nitrification-related genes and therefore nitrification rate. Our treatments had no obvious effects on N transformation and enzyme activity, but reduced gene abundance in bare soil. Biocrusts may facilitate N transformation while the degradation of moss crust caused by climate warming will dampen any regulating effect of biocrusts on the belowground microbial community. Furthermore, belowground microbial communities can mediate N transformation under ongoing warming and reduced precipitation by suppressing ammonification- and nitrification-related gene families, and by stimulating nitrification-related gene families involved in cyanobacteria-covered soil. This study provides a basis for identifying the functional genes involved in key processes in the N cycle in temperate desert ecosystems, and our results further highlight the importance of different biocrusts organisms in the N cycle in temperate deserts as Earth becomes hotter and drier.

In recent years, zinc based alloys as a new biodegradable metal material aroused intensive interests. However, the processing of Zn alloys micro-tubes (named slender-diameter and thin-walled tubes) is very difficult due to their HCP crystal structure and unfavorable mechanical properties. This study aimed to develop a novel technique to produce micro-tube of Zn alloy with good performance for biodegradable vascular stent application. In the present work, a processing method that combined drilling, cold rolling and optimized drawing was proposed to produce the novel Zn–5Mg–1Fe (wt%) alloy micro-tubes. The micro-tube with outer diameter of 2.5 mm and thickness of 130 µm was fabricated by this method and its dimension errors are within 10 µm. The micro-tube exhibits a fine and homogeneous microstructure, and the ultimate tensile strength and ductility are more than 220 MPa and 20% respectively. In addition, the micro-tube and stents of Zn alloy exhibit superior in vitro corrosion and expansion performance. It could be concluded that the novel Zn alloy micro-tube fabricated by above method might be a promising candidate material for biodegradable stent.

Summary Throughfall is known to be a critical component of the hydrological and biogeochemical cycles of forested ecosystems with inherently temporal and spatial variability. Yet little is understood concerning the throughfall variability of shrubs and the associated controlling factors in arid desert ecosystems. Here we systematically investigated the variability of throughfall of two morphological distinct xerophytic shrubs (Caragana korshinskii and Artemisia ordosica) within a re-vegetated arid desert ecosystem, and evaluated the effects of shrub structure and rainfall characteristics on throughfall based on heavily gauged throughfall measurements at the event scale. We found that morphological differences were not sufficient to generate significant difference (P

Characterizing the dynamics of plant water availability and the mechanisms through which plants access available water sources are key challenges in ecohydrology, especially in arid and semi-arid regions. The establishment of artificial revegetation of different ages has formed vegetation landscapes with different chronological sequences in the southeast margin of the Tengger Desert. As an important regulatory factor of the Soil Plant Atmosphere Continuum (SPAC) system, changes in vegetation characteristics will affect the water uptake of the entire ecosystem. In this study, the δ2H and δ18O isotope ratios in precipitation, plant water, soil moisture, and underground water, and the δ13C isotope ratio of plant leaves were measured using the stable isotope technique for artificial revegetation used to fix the sand dunes in the Tengger Desert. The purpose of this study was to establish the isotope content and characteristics of potential water sources for plants and leaf carbon isotopes, to clarify whether the soil moisture condition and duration of sand-fixing influenced the isotopic signal, and to identify the main water source for desert plants. The results indicated that air temperature and amounts of precipitation were two important influencing factors in precipitation isotope ratio change. Soil water isotopic ratios were different under different water conditions, and under the same water conditions, the vertical variation in soil water isotopes in a single area and spatial variation at any depth for different revegetation areas were all significant (p 70%) concentrated at a soil depth of 20–150 cm. For Caragana korshinskii plants, the 60–150 cm soil layer was the main water supply layer, while the water contribution layer of Artemisia ordosica plants focused on the 20–100 cm soil layer and the contribution rate of shallow soil water increased with the increase of sand fixation time in older revegetation areas, regardless of the species used for revegetation.

Effects of biological soil crusts (BSCs) on soil evaporation is quite controversial in literature, being either facilitative or inhibitive, and therein few studies have actually conducted direct evaporation measurements. Continuous field measurements of soil water evaporation were conducted on two microlysimeters, i.e., one with sand soil collected from bare sand dune area and the other with moss-crusted soil collected from an area that was revegetated in 1956, from field capacity to dry, at the southeastern edge of the Tengger Desert. We mainly aimed to quantify the diurnal variations of evaporation rate from two soils, and further comparatively discuss the effects of BSCs on soil evaporation after revegetation. Results showed that in clear days with high soil water content (Day 1 and 2), the diurnal variation of soil evaporation rate followed the typical convex upward parabolic curve, reaching its peak around mid-day. Diurnal evaporation rate and the accumulated evaporation amount of moss-crusted soil were lower (an average of 0.90 times) than that of sand soil in this stage. However, as soil water content decreased to a moderately low level (Day 3 and 4), the diurnal evaporation rate from moss-crusted soil was pronouncedly higher (an average of 3.91 times) than that of sand soil, prolonging the duration of this higher evaporation rate stage; it was slightly higher in the final stage (Day 5 and 6) when soil moisture was very low. We conclude that the effects of moss crusts on soil evaporation vary with different evaporation stages, which is closely related to soil water content, and the variation and transition of evaporation rate between bare soil and moss-crusted soil are expected to be predicted by soil water content.

Shrubs can induce spatial heterogeneity of soil nutrition and play a crucial role in dune stabilization in arid and semi-arid ecosystems. Soil enzyme activity can be used to investigate soil nutrient cycling, which further indicates soil recovery after sand stabilization. However, information regarding the effects of different shrubs on soil enzyme activity is scarce. To reveal and compare soil enzyme activities of two dominating sand-stabilizing shrubs, namely, Caragana korshinskii Kom. and Artemisia ordosica , we evaluated various hydrolase and oxidase enzymes involved in the carbon cycle (polyphenol oxidase, cellulose, and β-glucosidase) and nitrogen cycle (nitrate reductase and urease) at the shrub base (ASB), beneath the midcanopy (BMC), and in bare intershrub spaces (BIS). The results indicated that the sand-stabilizing shrubs improved soil nutrition and enzyme activity compared with bare soil. The effects varied with shrub type and position. Soil enzyme activities were greater in C. korshinskii than those in A. ordosica , and maximum enzyme activity was found at ASB, followed by BMC and BIS. All enzyme activities, except polyphenol oxidase, were higher at 0–5 cm depth. The enzyme activities exhibited similar seasonal patterns during the growing season, with relatively higher values in the summer rainy season than in the other seasons because of soil moisture and shrub growth. The results of this study suggest that sand-stabilizing shrubs, especially C. korshinskii , can increase soil nutrition and improve soil properties, thus playing a vital role in soil recovery in degraded areas of desert ecosystems.

Based on three different kinds of conductive paths in microstructure of soil and theory of electrochemical impedance spectroscopy (EIS), an integrated equivalent circuit model and impedance formula for soils were proposed, which contain 6 meaningful resistance and reactance parameters. Considering the conductive properties of soils and dispersion effects, mathematical equations for impedance under various circuit models were deduced and studied. The mathematical expression presents two semicircles for theoretical EIS Nyquist spectrum, in which the center of one semicircle is degraded to simply the equivalent model. Based on the measured parameters of EIS Nyquist spectrum, meaningful soil parameters can easily be determined. Additionally, EIS was used to investigate the soil properties with different water contents along with the mathematical relationships and mechanism between the physical parameters and water content. Magnitude of the impedance decreases with the increase of testing frequency and water content for Bode graphs. The proposed model would help us to better understand the soil microstructure and properties and offer more reasonable explanations for EIS spectra.

Soil moisture is a major component of the hydrologic cycle, being highly variable and nonlinear in space and time. Knowledge of soil moisture regime, especially at the root zone, is critical to the management of water resources and restoration of vegetation. As such, techniques that allow identifying and reducing the number of samples for soil moisture analysis are required. In this study, a spatial variability and temporal stability analysis were used to analyze the volumetric soil moisture content of root zone collected by neutron probe at 36 days during three years in Shapotou, China. The specific concern was to investigate the temporal stability of soil moisture at different depths in the soil profile, determine the effects of soil and vegetation characteristics on temporal stability, and to conduct such a study in an area larger than 1 km 2 . Additionally, we aimed to determine whether temporally stable sites are invariable at different depths, and compare with temporally stable shallow layer (0–6, 0–15, 0–30 cm) sites that are previously identified by Wang et al. (2013b) in the same study area. Results showed that the mean soil profile moisture demonstrated a moderate spatial variability which decreased with increasing soil moisture content at 0–60 cm depth; however, the variability of soil moisture and CV were both low and no significant correlations were found at 0–300 cm depth. A high temporal stability existed at two deeper soil layers compared with the soil surface observed by Wang et al. (2013b) . The sampling locations, representative of the dry conditions in the field, were always more temporally stable. Identified representative locations at two depths well-represented the mean soil moisture content in our study area larger than 1 km 2 . Furthermore, strong correlations at two soil layers revealed that spatial patterns of sampling points were preserved for all depths and that time stability of shallow measurements was a good indicator of deep soil layer time stability. Soil texture was the primary influence factor on soil profile moisture temporal stability and the dependence of soil moisture temporal stability on soil texture was consistent among different soil depths. Knowledge of the underlying stable soil moisture distribution could provide a useful basis for precise water management in arid areas.

Biological soil crust (BSC) is a key biotic factor in desert areas and can significantly alter nutrient cycling. The interaction between seasonal climatic change and BSC can further modify nutrient cycling. Thus far, limited information has been provided regarding the effect of BSC on net soil nitrogen (N) transformation and their seasonal pattern in temperate desert areas. Therefore, we assessed the seasonal patterns of net soil N mineralization and nitrification in three microhabitats (moss-covered, cyanobacteria–lichen-covered, and bare soils) from October 2011 to September 2012 by using an intact soil core in situ incubation method. Seasonal variations in inorganic N pools, net N mineralization, and nitrification rates were observed. The seasonal patterns of net N transformation rates were greatly modified by soil temperature and moisture (highest in August and lowest in January). During non-growing season, net N immobilization was observed in the three microsites; a higher inorganic N content was observed in the moss-covered soil than in other soils. BSC increased the net soil N transformation rates during the early growing season but reduced the inorganic N content during the peak growing season. On the basis of these findings, we concluded that seasonal variation in climate significantly affected N transformation. The colonization and the development of BSC stimulated N cycling and storage in arid desert systems.

The aim of this study was to evaluate canopy water storage (CWS) of the co-dominant shrubs in the revegetation of sand dunes in northwest China. Our results indicated that CWS differed among the xerophyte taxa studied. The average CWS increased exponentially with decreased raindrop size. The time course of CWS in terms of leaf area indicated that Artemisia ordosica attains its peak value of 0.48 mm within 170 min. The corresponding values for Caragana korshinskii and Hedysarum scoparium were 0.38 mm and 178 min, and 0.32 mm and 161 min, respectively, implying that A. ordosica had a higher CWS than C. korshinskii and H. scoparium. Dry biomass was a desirable predictor for estimation of CWS for C. korshinskii and H. scoparium, and shrub volume for A. ordosica. Our results show that the dependence of CWS on raindrop size varied in accordance with the shrub canopy structure. Editor Z.W. Kundzewicz

Background No gold standard currently exists for the diagnosis of general paresis (GP), thus often resulting in unnecessarily delayed therapeutic decision. Methods A retrospective chart review was performed for 85 inpatients with GP in Zhongshan Hospital, Medical College of Xiamen University, and the characteristics of their clinical profiles, serum and cerebrospinal fluid (CSF) examinations, neuroimaging examination, and electroencephalogram (EEG) data were analyzed. Results Among the 85 GP patients, the clinical symptoms that were frequently observed upon admission included a variety of psychiatric–behavioral symptoms and varying degrees of cognitive impairment. All of the patients had positive serum Treponema pallidum particle agglutination (TPPA) assays, 96.47% of the patients had positive CSF TPPA assays, and 41.18% of the patients had both CSF pleocytosis and elevated CSF protein levels. Focal atrophy in one cerebral region or in multiple regions was evident in neuroimages. The EEG data primarily showed slightly abnormal EEG activity. Conclusion These results demonstrate the complexity of the clinical characteristics of GP and highlight the importance of early diagnosis.

Dysregulation of microRNAs (miRs) can contribute to cancer development and progression. In the present study, the function and underlying molecular mechanisms of miR-320 in breast cancer tumorigenesis and progression were investigated. The results of a reverse transcription-quantitative polymerase chain reaction analysis demonstrated that miR-320 was frequently downregulated in breast cancer tissues compared with adjacent normal tissues. In addition, knockdown of miR-320 in breast cancer cell lines promoted cell proliferation and invasion in vitro, whereas miR-320 overexpression had the opposite effect. Furthermore, a Dual-Luciferase reporter assay indicated that SRY-box 4 (SOX4) is a direct target of miR-320, and the restoration of SOX4 in miR-320-overexpressing cells attenuated the tumor-suppressive effects of miR-320. Collectively, these results indicated that miR-320 acts as a tumor suppressor in breast cancer tumorigenesis and progression.

Pentatricopepetide repeat (PPR) proteins are a large family of RNA-binding proteins involved in RNA metabolism in plant organelles. Although many PPR proteins have been functionally studied, few of them are identified with a function in mitochondrial RNA stability. By using a reverse genetic approach, we characterized the role of the mitochondrion-targeted PPR78 protein in nad5 mature mRNA stability and maize (Zea mays) seed development. Loss of PPR78 function leads to a dramatic reduction in the steady-state level of mitochondrial nad5 mature mRNA, blocks the assembly of complex I in the electron transport chain, and causes an arrest in embryogenesis and endosperm development. Characterization of a second strong allele confirms the function of PPR78 in nad5 mRNA accumulation and maize seed development. The generation of mature nad5 requires the assembly of three distinct precursor RNAs via trans-splicing reactions, and the accumulation of nad5T1 precursor is reduced in the ppr78 mutants. However, it is the instability of mature nad5 rather than nad5T1 causing loss of the full-length nad5 transcript, and degradation of nad5 losing both translation start and stop codons is enriched in the mutant. Our data imply the assembly of mature nad5 mRNA precedes the protection of PPR78.

Change in electrical conductivity is considered apotential indicator for the on-line monitoring of wine aging accelerated by ultrasound, as determined inour previous study; however, the exact mechanism of change is currently unclear. In this study, the ion content and the total ionic strength were analyzed by ion-exchange chromatography to investigate the change mechanism of the electrical conductivity of wineunder ultrasound irradiation. The results indicate that the changes in wine electrical conductivity during ultrasound treatment correlate with the changes in the cations (Na+, K+, Ca2+, Mg2+, and NH4+) and in the anions from the organic acids (malic acid, citric acid, tartaric acid, oxalic acid, and formic acid) and inorganic acids (Cl−, SO42−, and PO43−), especially for the ionic strength of the wine. Overall, electrical conductivity may be used to reflect the chemical reactions related to wine aging to a certain extent becausethe reactions can be initiated by the conversion of cations and by the degradation or auxiliary functionof organic acids.