Search

Objective·To examine the expression level of protein arginine methyltransferase 6 (PRMT6) in breast carcinoma tissues and to assess its impact on the proliferative and migratory behaviors of breast cancer cells.Methods·The PRMT6 transcriptome sequencing data between 33 tumor tissues and normal tissues from The Cancer Genome Atlas (TCGA) database was analyzed through the R language. The gene expression profile interactive analysis (GEPIA2) online database was used to analyze the difference of PRMT6 expression in normal breast tissues and breast cancer tissues. By using the immunohistochemistry (IHC) data of human normal breast tissues and breast cancer tissues from Human Protein Atlas (HPA) database to analyze the protein expression of PRMT6. IHC was used to detect the expression of PRMT6 in breast cancer tissues and paired para-tumor tissues from 27 clinical samples. After PRMT6 was knocked down with small interfering RNA (siRNA) in MDA-MB-231 and MCF-7 cells , the expression of PRMT6 was detected by qRT-PCR and Western blotting. The proliferation ability of breast cancer cells was measured with cell counting kit-8 (CCK-8) assay and colony formation assay. The effect of PRMT6 on the migration ability of breast cancer cells was detected by wound healing assay and Transwell assay. By using the RNA-sequence data from GSE210948 of Gene Expression Omnibus (GEO) database, differentially expressed genes were analyzed in control and low expression groups of PRMT6. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis was performed to reveal the signaling pathways associated with PRMT6. Cell cycle analysis was detected by flow cytometry. The expressions of cyclin D1 and EMT-related proteins (E-cadherin, N-cadherin and Vimentin) were detected by Western blotting after knocking down PRMT6.Results·Bioinformatics analysis and IHC results showed that PRMT6 was highly expressed in breast cancer tissues compared with normal tissues (P=0.000) and para-tumor tissues (P=0.001). qRT-PCR and Western blotting results verified that the siRNA significantly reduced the expression level of PRMT6 in MDA-MB-231 and MCF-7 cell lines compared with the control group (mRNA: P=0.006, P=0.004; P=0.001, P=0.043. Protein: P=0.035, P=0.001; P=0.003, P=0.002). After knocking down PRMT6, the proliferation (P=0.014, P=0.000; P=0.003, P=0.003) and migration (P=0.000, P=0.000; P=0.000, P=0.002) ability of breast cancer cells were inhibited significantly . The KEGG pathway enrichment analysis showed that the expression of PRMT6 affected the cell cycle pathway. After knocking down PRMT6, the expression of cyclin D1 decreased in protein level (P=0.021, P=0.000; P=0.034, P=0.014) and transcription level (P=0.036, P=0.001; P=0.044, P=0.000). Knock down of PRMT6 increased the number of cells in G0/G1 phase (P=0.000; P=0.003) and decreased the number of cells in G2/M phase of the cell cycle . The expression level of E-cadherin increased (P=0.002, P=0.012; P=0.043, P=0.003), while the expression levels of N-cadherin (P=0.004, P=0.041; P=0.032, P=0.034) and Vimentin (P=0.028, P=0.005; P=0.024, P=0.001) decreased in PRMT6 knockdown cells .Conclusion·PRMT6 is highly expressed in breast cancer, which can promote the proliferation and migration of breast cancer cells.

AIM: To investigate the effects of placement of the absorbable packing material Nasopore around the anastomosis site on postoperative re-bleeding, discomfort, and on the success rate of endoscopic dacryocystorhinostomy(En-DCR).METHODS: Prospective randomized controlled study. A total of 101 patients(101 eyes)diagnosed with chronic dacryocystitis that underwent En-DCR in the ophthalmology department, Renmin Hospital, Hubei University of Medicine from November 2020 to October 2021 were collected. The patients were randomly divided into two groups according to whether they were packed with Nasopore at the end of operation, namely, the packed group(49 eyes)and the non-packed group(52 eyes). The postoperative follow-up was 9 mo, the degree of re-bleeding, discomfort, and postoperative success rate(including anatomical success rate and functional success rate)were compared between the two groups of patients.RESULTS: This study included 94 patients, including 45(45 eyes)and 49(49 eyes)in packed group and non-packed group, respectively. The En-DCR was performed successfully in all patients. Postoperative re-bleeding occurred in 1 eye(2%)in the packed group, and 9 eyes(18%)in the non-packed group(P0.05). The postoperative functional success rate was 89%(40/45)in the packed group and 86%(42/49)in the non-packed group(P>0.05). Other complications such as orbital fat prolapse, cerebrospinal fluid leakage, sinusitis, visual impairment and double vision were not observed in all patients during the follow-up.CONCLUSION: Nasal packing absorbable material Nasopore around the anastomosis at the end of En-DCR operation can reduce postoperative re-bleeding and postoperative discomfort of patients, and it has no obvious effect on the postoperative success rate.

Cold stimulation has been shown to regulate glucose, lipid, and amino acid metabolism, while also increasing heat production and energy expenditure in the body. Disordered energy metabolism is a key factor in the onset and progression of chronic metabolic conditiones such as diabetes, obesity, and cardiovascular disease. Recent research has unveiled the myriad pathways through which cold stimulation affects human energy metabolism. This article provides an overview of how cold stimulation affects energy metabolism across the three major metabolic pathways. Furthermore, it explores the implications and potential therapeutic applications of cold stimulation in the prevention and treatment of various metabolic diseases.

The application of low-condensation diesel in cold regions with extremely low ambient temperatures (−14 to −29 °C) has enabled the operation of diesel vehicles. Still, it may contribute to heavy haze pollution in cold regions during winter. Here we examine pollutant emissions from low-condensation diesel in China. We measure the emissions of elemental carbon (EC), organic carbon (OC), and elements, including heavy metals such as arsenic (As). Our results show that low-condensation diesel increased EC and OC emissions by 2.5 and 2.6 times compared to normal diesel fuel, respectively. Indicators of vehicular sources, including EC, As, lead (Pb), cadmium (Cd), chromium (Cr), nickel (Ni), and manganese (Mn), increased by approximately 20.2–162.5% when using low-condensation diesel. Seasonal variation of vehicular source indicators, observed at road site ambient environments revealed the enhancement of PM2.5 pollution by the application of low-condensation diesel in winter. These findings suggest that −35# diesel, a low-cetane index diesel, may enhance air pollution in winter, according to a dynamometer test conducted in laboratory. It raises questions about whether higher emissions are released if −35# diesel is applied to running vehicles in real-world cold ambient environments.

Geometries, stabilities, bonding nature, and vibrational properties of bimetallic KMgn0/– (n = 2–12) clusters have been comprehensively studied by CALYPSO code within DFT calculations. The results reveal the structural transition from 2D to 3D at n = 3. From n ≥ 8, the geometry of KMgn0/– transfers a hollow framework with a six-atom triangular prism unit. By comparing the geometry of neutral and anionic KMgn0/– clusters, most of them are structurally different. In all doped clusters, the K atoms tend to localize on the convex positions of the skeleton and play the role of electron donors. Two highly stable clusters KMg9 and KMg9– have been found, and studies showed that the prominent stability benefits from their compact magnesium structural motifs and quasi-full/full shell electronic configurations. Additionally, bonding nature analysis reflects there is much weak K-Mg interaction than Mg-Mg interaction in the KMg90/– clusters. The spectroscopic properties based on the PES, IR and Raman spectra have also been discussed.

The energy and environmental crisis pose a great challenge to human development in the 21st century. The design and development of clean and renewable energy and the solution for environmental pollution have become a hotspot in the current research. Based on the preparation of transition metal phosphates, transition metals were used as raw materials, Prussian blue-like NiFe(CN)6 as a precursor, which was in situ grown on nickel foam (NF) substrate. After low temperature phosphating treatment, a bimetallic phosphide electrocatalyst (NiFe)2P/NF was prepared on NF substrate. Using 1 mol·L−1 KOH solution as a basic electrolyte, based on the electrochemical workstation of a three-electrode system, the electrochemical catalytic oxygen evolution performance of the material was tested and evaluated. Experiments show that (NiFe)2P/NF catalyst has excellent oxygen evolution performance. In an alkaline medium, the overpotential required to obtain the catalytic current density of 10 mA·cm−2 is only 220 mV, and the Tafel slope is 67 mV·dec−1. This is largely due to: (1) (NiFe)2p/NF nanocatalysts were well dispersed on NF substrates, which increased the number of active sites exposed; (2) the hollow heterostructure of bimetallic phosphates promotes the electron interaction between (NiFe)2P and NF, increased the rate of charge transfer, and the electrical conductivity of the material is improved; and (3) theoretical calculations show that (NiFe)2P/NF hollow heterostructure can effectively reduce the dissociation barrier of water, promote the dissociation of water; furthermore, the kinetic reaction rate of electrocatalytic oxygen evolution is accelerated. Meanwhile, the catalyst still has high activity and high stability in 30 wt% concentrated alkali solution. Therefore, the construction of (NiFe)2P/NF electrocatalysts enriches the application of non-noble metal nanomaterials in the field of oxygen production from electrolytic water.

[Objective] Liquid hydrogen has garnered significant interest for its high volumetric density and hydrogen purity. However, it easily evaporates during transportation due to its low boiling point, thus causing hydrogen loss. In this case, cryogenic insulation technology becomes essential for facilitating liquid hydrogen pipeline transportation. [Methods] The analysis of heat leakage in the pipeline's annulus considered three modes of heat transfer: solid heat conduction, gas heat conduction, and radiant heat exchange. Since the solid heat conduction primarily arises from the thermal insulation support structure within the annulus, an overview was provided on the heatconducting properties of various support structures. In addition, maintaining the vacuum level of annulus interlayer can effectively weaken the heat leakage caused by gas heat conduction. The impact of vacuum multilayer insulation on cryogenic insulation is a current focus of research. The prediction model and arrangement mode of vacuum multilayer insulation, as well as uniform density multilayer insulation(UD-MLI) and variable density multilayer insulation(VD-MLI) were examined and analyzed in this study. [Results] By optimizing support shape and minimizing heat transfer paths while reducing contact points and areas between the support and inner/outer pipe, heat leakage can be effectively decreased while ensuring stress intensity. Further study on cost-effective and high-efficiency getters is the most direct and effective approach to maintaining vacuum levels. Vacuum multilayer insulation technology has been extensively utilized in cryogenic equipment to significantly diminish solid and gas heat conduction as well as radiant heat exchange. Both UD-MLI and VD-MLI feature the optimal layer density configuration to minimize heat leakage, with VD-MLI offering superior insulation performance and a weight advantage. [Conclusion] Optimizing annulus geometry and heat transfer modes, along with developing high-efficiency hydrogen getters, are vital for advancing annulus insulation technology in cryogenic liquid hydrogen transportation pipelines and minimizing hydrogen loss during transportation.

[Objective] Metal hydride hydrogen storage reactors serve as core components in solid-state hydrogen storage techniques.During the reversible hydrogen absorption and desorption reactions of metal hydrides, significant heat exchange occurs, leading to uneven temperature distribution within the reactors and resulting in adverse effects on the hydrogen storage performance and service life of the reactors. Therefore, effective thermal management is recognized as one of the key technologies to improve the efficiency and stability of metal hydride hydrogen storage reactors. [Methods] This paper aims to review the research progress in the field of thermal management for metal hydride hydrogen storage reactors both in China and abroad. To this end, an extensive investigation of available literature was conducted to summarize the principle of hydrogen absorption-desorption reactions in metal hydrides. The heat transfer process within hydrogen storage reactors was examined, leading to the division of the heat exchange process into three stages: heat transfer in the hydrogen storage materials; heat exchange between the hydrogen storage materials and the wall surface of the hydrogen storage reactors; heat exchange between the wall surface of the hydrogen storage reactors and the ambient environment. Furthermore, thermal management methods that enhance heat exchange efficiency in each stage were categorized. [Results] Through an analysis of the heat exchange performance in various thermal management methods, this review presents an overview of the research progress in these approaches, including the use of heat exchange tubes, fins, and phase-change materials. The evaluation of hydrogen absorption and desorption rates as crucial metrics allows for a comparison of their advantages and disadvantages. Additionally, the review summarizes the existing challenges in this field and envisions the prospects for future trends in thermal management for metal hydride hydrogen storage reactors. [Conclusion] Different thermal management methods have their pros and cons. Therefore, it is essential to select and optimize specific thermal management methods based on the scale and intentions of metal hydride hydrogen storage reactors, to design efficient and compact thermal management systems. The outcomes of this research lay a foundation for the large-scale and engineering applications of solid-state hydrogen storage techniques.

An experimental study on the recovery of phosphorus resource was carried out for the resource application of sludge residue. The effects of acid species and different leaching conditions on the leaching of phosphorus from sludge residue were systematically analyzed，and the effects of pH and n（Mg）∶n（N）∶n（P） on the synthesis of struvite from the solution containing phosphorus were investigated. The results showed that the leaching rate of phosphorus from sludge residue could reach 96.9% under the conditions of liquid/solid ratio of 20 mL/g，hydrochloric acid concentration of 0.4 mol/L and leaching time of 4 h. Moreover，the crystallization rate of the phosphorus purification solution purified by cation exchange resin could reach 98.1% at pH=10，n（Mg）∶n（N）∶n（P）=1.2∶1.2∶1，and the heavy metal content in the obtained struvite met the standard limit of Organic and Inorganic Compound Fertilizer （GB/T 18877-2020）. In summary，79% of phosphorus in sludge residue could be recovered after phosphorus extraction，purification and crystallization.

In order to obtain the static strength of small modulus plastic gears, a small modulus plastic gear static strength test bench is developed, which can accurately measure the static strength of plastic gears under different modulus, speeds and materials. The working principle, constitution and the design of tooling parts of the test bed are mainly introduced. The test bench is composed of the mechanical system, the electric control system and the measuring software. The electric control system and the measuring software mainly adopt the horizontal structure, which is of the compact structure, with small space occupation, uses simple fixture design and is easy to measure. The static strength test is carried out, and the static strength using different materials is briefly tested. The experimental equipment can realize the measurement of the static strength of the small module plastic gear, which solves the problems of inaccurate static strength test of the small module gear and the difficulty of test clamping, and provides theoretical value and practical application value for the design of the plastic gear in the future.

The Cloud-based Liquid Pipeline Simulator(CloudLPS) is a universal liquid pipeline simulation software with completely independent intellectual property rights in China. Herein, its applications are introduced. Its operation processes from pipeline network modeling and simulation calculation to result viewing are elaborated based on a specific case. Then, calculations are performed with CloudLPS for various operating conditions, including steady-state transportation, transient water hammer, and alternating transportation of cold and hot oil, after which the results are compared with those obtained from the foreign commercial software SPS and TLNET, making the discovery that the CloudLPS has comparable accuracy in terms of hydrodynamic and thermal simulations. Subsequently, CloudLPS is applied to solve practical pipeline problems such as normal-temperature transportation, heated transportation, and alternating transportation of cold and hot oil. By comparing the results of these practical applications with the field data, it is proven that the CloudLPS can meet the requirements of the actual engineering calculations. Finally, the two special features of CloudLPS, including visual control logic construction and energy consumption optimization, are presented. The results of this paper indicate that CloudLPS is of great competence in hydrodynamic and thermal simulations, logic control, and energy consumption optimization.

Ceramic matrix composites are desirable materials for the hot end components of high performance generation aeroengines due to their high temperature resistance, low density and excellent high temperature mechanical properties. However, when exposed to combustion environment, the ceramic matrix composites are subjected to serious water vapor corrosion. Thus, environmental barrier coatings are indispensable to apply to their surfaces to extend their service life.The rare earth silicate has become the primary candidate material for the new generation of environmental barrier coating materials because of its suitable thermal expansion coefficient with the substrate, outstanding water vapor corrosion resistance and high temperature stability. The characteristics, preparation techniques and typical service performance of rare earth silicates were reviewed in this paper, with focus on their classification, thermal/physical properties, as well as the damage and failure mechanisms during high-temperature corrosion processes. Finally, the research directions of high entropy design of multi-component rare-earth silicates and the design of new thermal/environmental barrier coating systems were proposed. This paper aims to provide useful references for the further application of rare earth silicate materials.

Cold stimulation has been linked to acute myocardial infarction and other cardiovascular diseases. Residents in the frigid zones, such Heilongjiang Province, experience a higher incidence of adverse cardiovascular events during winter, posing a significant health threat and increasing the overall medical burden. Cold stimulation serves as an detrimental stressor, inducing inflammation in the body. Therefore, understanding the role of inflammatory responses induced by cold stimulation in the occurrence and development of cardiovascular diseases is of paramount importance. Given the impact of cold on inflammation in cardiovascular diseases and the expanding array of anti-inflammatory methods for the treatment of cardiovascular diseases, delving into the inflammatory responses mediated by can significantly complement cardiovascular disease management. This review explorest the synergistic relationship between cold stimulation and inflammation induction, elucidating how this interplay influences the occurrence and progression of cardiovascular diseases.

A domestic cloud simulation software platform named CloudLPS(Cloud-Based Liquid Pipeline Simulator) with complete independent intellectual property rights is developed, aiming to eliminate the long-term reliance on foreign commercial simulation softwares for liquid pipeline simulation. The platform is built on a B/S cloud architecture and is designed with the goals of accuracy, speed, simple interfaces, and convenient interaction. In terms of models, in addition to conventional flow and heat transfer models, CloudLPS also introduces the advanced mathematical models such as the non-Newtonian fluid model and the cross-dimensional thermal model, which enables the software to have the capability of hydraulic and thermal simulations for condensable and high-viscosity crude oil pipelines. Furthermore, the software also introduces some efficient simulation methods,such as adaptive mesh, dynamic mesh, divide and conquer, and heterogeneous parallelization, to facilitate the rapid calculation of large and complex pipe network system. In terms of functionality, it primarily offers users a variety of business and composite business function support to crude oil and refined oil pipeline networks, such as steady-state and unsteady-state simulations of pipeline network system, logic control, water hammer analysis,batch tracking, shutdown and restart, energy consumption optimization, and more. Additionally, the software provides a user-friendly visual modeling and result data display and analysis environment. CloudLPS serves as a fundamental software tool that plays a supportive role in various aspects of pipeline network system, such as planning and designing, operation scheduling, risk decision-making, and other business scenarios. Its application holds significant importance in ensuring the safe and economic operation of liquid pipelines in China.

China has identified the synergistic reduction of pollution and carbon emissions as a crit ical component of its environmental protection and climate mitigation efforts. An assessment of this synergy can provide clarity on the strategic management of both air pollution and carbon emissions. Due to the extensive regional differences in China, the spatial effects of influencing factors on this synergy exhibit variation across different provinces. In this study, the reduction indexes of PM2.5 and CO2 were calculated based on their reduction bases, reduction efforts, and reduction stabilities across provinces. Then, the synergistic reduction effect was assessed using an exponential function with the PM2.5 reduction index as the base and the CO2 reduction index as the exponent. Next, the MGWR model was applied in order to analyze the influencing factors of the synergistic reduction effect, considering natural settings, socioeconomic conditions, and external emission impacts. Finally, the k-means clustering method was utilized to classify provinces into different categories based on the degree of impact of each influencing factor. The results indicated that air circulation, vegetation, tertiary industry ratio, and emission reduction efficiency are major impact indicators that have a positive effect. The topography and emissions from neighboring provinces have a statistically significant negative impact. The spatial influences of different factors exhibit a distribution trend characterized by a high-high cluster and a low-low cluster. A total of 31 provinces are divided into three categories, and suggestions on the corresponding category are proposed, to provide a scientific reference to the synergistic reduction of PM2.5 and CO2.

[Purpose/Significance] In the ever-evolving realm of higher education, the pronounced significance of methodically evaluating talented people in scientific and technological (S&T) fields within academic institutions has risen to paramount importance. This research endeavors to intricately dissect the manifold factors of shaping the evaluation of talented people in Chinese colleges and universities. Simultaneously, it seeks to meticulously design a comprehensive indicator system custom-tailored to this unique milieu. The dual objective encompasses unraveling the intricate dynamics underpinning the evaluation of talented people in S&T fields and invigorating talent cultivation and igniting innovation in scientific research across the academic landscape. [Method/Process] Guided by a meticulous review of both domestic and international advancements in the domain of the evaluation of talented people in S&T fields in higher education, this paper diligently dissects the intricate interplay of systems, theories, methodologies, and evaluation frameworks within Chinese academic institutions. By meticulously addressing associated challenges, conducting extensive discourse on influential variables, and meticulously examining facets of S&T prowess assessment within universities, this study culminates in the construction of an evaluation indicator system tailored to the unique nature of institutions of Chinese higher education. [Results/Conclusions] The findings echo the existence of critical gaps in the ongoing evaluation of talented people in S&T fields within Chinese universities. These noteworthy limitations encapsulate the intricate equilibrium sought between objectivity and subjectivity, the imperative for indicators to be comprehensive yet operationally feasible, the mandate for assessment to be dynamic and timely, and the essentiality of embracing multi-dimensional evaluation. This compels us to chart a trajectory towards the future where the development of an evaluation system of talented people in S&T fields rests on the bedrock of data-driven and intelligent assessment paradigms. This envisioned system must incorporate multi-dimensional evaluation approaches, integrate behavioral assessment modalities, deploy dynamic evaluation mechanisms, and, equally, factor in dimensions of social influence, sustainability, and internationalization. As we navigate this academic journey, further empirical research and meticulous indicator validation exercises must be undertaken within the realm of the evaluation of talented people in S&T fields in Chinese higher education institutions. These empirical undertakings, by virtue of their in-depth insights, are poised to illuminate our path forward, serving as compasses guiding the development of talented people in S&T fields in China's academic sphere. The journey towards comprehensive evaluation necessitates data-driven intelligence, multifaceted assessment perspectives, and a clear alignment with the pulse of societal dynamics and global imperatives. The culmination of these efforts will invariably shape the trajectory of the progression of talented people in S&T fields, ensuring that they remain at the vanguard of innovation and advancement. In essence, the evaluation of talented people within Chinese higher education institutions is primed for an evolution characterized by acumen, innovation, and internationalization. Through the harmonious amalgamation of rigorous research methodologies and adaptive frameworks, the academic community is poised to craft a transformative path that enriches talent development, propels research breakthroughs, and fuels the advancement of knowledge-based societies.

This paper presents a hybrid physics-data-driven optimization model for grassland grazing management. It comprehensively assesses essential factors in the Abaga Banner grassland ecosystem, including soil moisture, vegetation biomass, desertification degree index, and soil compaction. Through a thorough analysis, the impacts of grazing patterns and intensity on the grassland’s physical characteristics and biomass are studied. Employing genetic algorithms, an optimal grazing model is formulated to minimize soil desertification throughout the year. The paper aims to contribute to grassland ecology restoration and ensure sustainable livelihoods for local herdsmen, offering a scientific foundation for promoting the sustainable growth of grassland husbandry.

High temperature solid oxide electrolysis cell (SOEC) is a new type of high-efficiency electrochemical energy transfer device with high energy transfer efficiency, high reaction rate and wide application scenarios. It has enormous potential in the fields including production of low-cost green hydrogen and carbonaceous product with high added values. Nitrogen oxide treatment and ammonia synthesis may also be a promising direction of application. SOEC is expected to play an important role in the low-carbon transformation of energy, chemical industry, transportation and other fields. Based on the latest progress of SOEC in the fields of hydrogen production, oil production, nitride treatment and ammonia production, the development status of SOEC was systematically summarized, and the key directions for future development were prospected.

Traditional Chinese medicine (TCM) equipment is the industry representative possessing independent intellectual property rights and unique Chinese characteristics. By integrating TCM and information technology, TCM equipment is experiencing an unprecedented period of opportunity. Here, based on the practical significance, we reviewed the recent series of policies to promote the TCM equipment development. In accordance, we analyzed the current main problems and causes, and finally put forward some policy demand and suggestions to boost TCM equipment industry.

Introduction: Plaque rupture in atherosclerosis contributes to various acute cardiovascular events. As a new sulfide-containing donor, S-propargyl-cysteine (SPRC) has been reported to play a beneficial role in cardioprotection, potentially through its anti-inflammatory, anti-oxidative and anti-atherogenic activities. Our previous study observed an increase in eNOS phosphorylation in endothelial cells. However, it remains unclear whether SPRC influences vascular smooth muscle cells (VSMCs) within the plaque and if this effect contributes to plaque stabilization.Methods: An atherosclerotic unstable plaque mouse model was established by subjecting ApoE−/− mice to tandem stenosis of the right carotid artery along with a Western diet. Daily SPRC administration was conducted for 13 weeks. Plaque morphology and stability were assessed using MRI scanning and histopathological staining. In our in vitro studies, we stimulated human artery vascular smooth muscle cells (HAVSMCs) with platelet-derived growth factor-BB (PDGF-BB), both with and without 100 μM SPRC treatment. Cell phenotype was assessed using both Western blot and Real-time PCR. Cell proliferation was assessed using the BrdU cell proliferation kit and immunofluorescence of Ki-67, while cell migration was measured using scratch wound healing and transwell assay. MiR-143-3p overexpression and knockdown experiments were used to investigate whether it mediates the effect of SPRC on VSMC phenotype.Results and Discussion: SPRC treatment reduced plasma lipid levels, increased collagen content and decreased cell apoptosis in atherosclerotic plaques, indicating improved plaque stability. Both in vivo and in vitro studies elucidated the role of SPRC in preserving the contractile phenotype of VSMCs through up-regulation of miR-143-3p expression. Furthermore, SPRC suppressed the pro-proliferation and pro-migration effects of PDGF-BB on HAVSMCs. Overall, these findings suggest that the inhibitory effect of SPRC on phenotype switch from contractile to synthetic VSMCs may contribute to its beneficial role in enhancing plaque stability.

This paper takes Taigemiao Mining Area in the middle and upper reaches of the Yellow River Basin as the research object, analyzes the relationship between the spatial structure of water-bearing（ｉｓｏｌａｔｅｄ） layer and the development height of water flowing fractured zone, excavates the main control factors affecting the water loss of aquifer in the study area, and introduces the effective thickness index of residual bedrock to construct the water loss risk evaluation system of shallow Cretaceous aquifer. Based on the analytic hierarchy process-entropy weight comprehensive discrimination method, the weight of each main control factor is calculated, and the quantitative evaluation model of the disturbance of deep coal seam mining to shallow aquifer is established. By overlaying the control factor diagrams with different weights, the zoning map of water loss risk in deep coal seam mining is obtained. The zoning results are verified by the measured water inflow of surrounding mines. The results show that the aquifer thickness, water pressure, unit water inflow, and the thickness of residual water-resisting protective layer under the influence of mining are the main controlling factors for the water loss of aquifer in the main coal seam. The northwest and northeast corners of the mining area have good water abundance, and the thickness of residual water-resisting bedrock is thin, resulting in a certain water loss risk in the region. The research results are basically consistent with the actual situation in the field.

Objective To profile the genome-wide distribution of H3K27ac-marked enhancers in gastric intestinal metaplasia (IM) tissues and to investigate the potential regulatory mechanism of enhancers on IM. Methods IM tissues from 41 patients and normal gastric mucosa from 21 healthy individuals were collected for analysis using Cleavage Under Targets and Tagmentation (CUT&Tag) sequencing and RNA sequencing. The number and signal intensity of H3K27ac modifications in the 2 tissues were compared to reveal the reprogramming characteristics of the enhancers in the IM tissues. Meanwhile, the underlying regulatory mechanism of enhancer-associated transcription factors on the expression of IM-related genes were investigated. Results As compared with normal gastric mucosa, the number and signal intensity of H3K27ac modifications were significantly elevated in the IM tissues, and the reprogramming areas were mainly located in the enhancers. The enhancers with greatly increased activity accounted for 92.4% of all variant enhancer loci in IM, which improved the expression of many intestinal epithelium-related genes, leading to altered phenotypes and biological properties of metaplastic mucous cells. The binding motifs of 14 transcription factors including CDX2 were identified by enrichment analysis in the enhancer loci. These transcription factors were up-regulated in the IM tissues and thus constituted a transcriptional regulatory network, and may play an important role in enhancer activating IM-related genes. Conclusion Through integration analysis of epigenetic and transcriptomic sequencing, enhancer reprogramming is identified as a critical epigenetic modification feature in gastric IM. Multiple transcription factors like CDX2 might be recruited to the enhancer loci, and then generate a regulatory network to synergistically up-regulate the expression of IM-related genes.

Objective To systemically characterize the genome-wide distribution of H3K27me3-marked silencers in gastric intestinal metaplasia (IM) tissues and to elucidate a novel epigenetic regulatory mechanism of IM. Methods Gastric antrum mucosa samples were collected from 22 healthy individuals and 39 patients with IM. The modification of H3K27me3 and the transcriptome characteristics were detected by Cleavage Under Targets and Tagmentation (CUT&Tag) sequencing and RNA sequencing, respectively. Various bioinformatics tools, such as Ngsplot, ChIPseeker, MAnorm2, GGBiPlot, edgeR, Homer and others were applied for analyzing the silencer signal and its regulation on gene expression of IM tissues. Results CUT&Tag sequencing and RNA sequencing data were of great quality, and there was no significance in the whole-genome distribution of H3K27me3 modification between normal gastric mucosa and IM tissues. Genome-scale distribution of H3K27me3-marked silencers was also portrayed, which revealed weakened silencer signal intensity in IM tissues (P < 0.05). Principal component analysis (PCA) showed that the silencer characteristics in IM were greatly different from those in normal tissues, indicating extensive silencer remodeling in the IM tissues. Combined with RNA sequencing analysis, silencer remodeling was closely associated with the increased expression of CDX1 and other IM-related genes, as well as with the possible activation of IM-related metabolic pathways. In addition, loss of silencer signal recruited various transcription factors such as ATOH1 (P < 0.01) and ONECUT2 (P < 0.01) to form a regulatory network, which were highly expressed in IM tissues, regulating the expression of CDX1 and other key genes of IM, and affecting cell biological processes like coll fate commitment. Conclusion Integration analysis of epigenetic and transcriptomic sequencing data discovers silencer remodeling as a hallmark epigenetic feature in gastric IM tissues. Loss-of-function in silencer loci may recruit IM-regulatory transcription factors such as ATOH1 and ONECUT2, which may generate a network to impact on the expression of key genes and the metabolism of IM cells.

Objective: A Colon Cleanliness Rating Algorithm (CCRA) based on colonoscopy image analysis is proposed in this paper, in order to solve the problem that the results of Colon Cleanliness (or Bowel Preparation Quality) rating caused by manual inspection are inconsistent. Methods: Firstly, CCRA intercepts images from the colonoscopy video. Secondly, each colonoscopy image's stool area is segmented by U-Net to obtain the 2-classification segmentation results. Finally, the colon cleanliness is obtained by comparing the average area of the stool area with the standard proportion. Results: After testing, the pixel accuracy of the U-Net model is 97.02 %, IoU is 83.67 %, accuracy is 92.17 %, recall is 90.21 %, F1-Score is 90.95 %. The accuracy of CCRA is 92.45 %–99.275 % Conclusion: The experimental results show that the CCRA proposed in this paper can quickly and accurately output the colon cleanliness rating of patients without manpower.

In coal mine safety evaluation, the determination of index weight is particularly critical, which plays a decisive role in the evaluation results. At present, there are some shortcomings in the evaluation of internal fire in coal mine, such as few index selections, difficulty in determining index weight, unclear definition of evaluation grade and so on, which affects the accuracy of the evaluation results. On the basis of these, the evaluation system of coal mine internal fire is constructed based on four factors: coal seam geological occurrence, development and mining technology, coal seam spontaneous combustion tendency, ventilation condition and goaf management, and coal seam thickness, coal seam inclination and other 24 factors as the third-level index. By using the decision model and the Euclidean distance function, the subjective weight of the analytic hierarchy process and the objective weight of the entropy method are effectively integrated to obtain a more reasonable combined weight. The combined weighting-matter-element extension model is used to evaluate the internal fire in a coal mine. The result is “safer” grade, which is consistent with the engineering practice. The model is compared with the traditional evaluation method, the model is not only accurate, but also can reflect the degree of deviation within the evaluation grade, and can effectively improve the accuracy of the evaluation results.

Objective To investigate the effect of the intervention of Hufu Tongbian scraping combined with Traditional Chinese Medicine(TCM) iontophoresis on relieving symptoms of Fontaine's stage II arteriosclerosis obliteration(ASO). Methods Totally 130 cases of hospitalized patients with Fontaine's stage II ASO were randomly divided into 4 groups: group A (control group, n=31) and group B (Hufu Tongbian Scraping group, n=31), group C (TCM Iontophoresis group, n=34), group D (combination group, n=34). Group A was given specialist nursing, group B was given specialist nursing combined with Hufu Tongbian scraping intervention, group C was given specialist nursing combined with TCM iontophoresis intervention, group D was given specialist nursing combined with Hufu Tongbian scraping and TCM iontophoresis intervention. Symptom quantitative score and Visual Analogue Score (VAS)of pain were measured before and 15 days. after intervention. Results After intervention, there were significant differences in each dimension of symptom score and pain score among the four groups (P＜0. 05). the scores of each dimension of symptom score and pain score in groups B, C and D were decreased after intervention compared with those before intervention(P＜0. 05), and the scores in the three groups were lower than those in group A after intervention (P＜0. 05). After intervention, the scores of each dimension of symptom score and pain score in group D were lower than those in groups B and C (P＜0. 05), but there was no significant difference between groups B and C after intervention (P＞0. 05). Conclusion The intervention Hufu Tongbian scraping combined with TCM iontophoresis can significantly reduce the symptom score of ASO patients, relieve the symptoms of acid swelling and numbness, relieve the pain of patients, promote comfort, and provide better nursing for ASO patients. (目的 探讨虎符铜砭刮痧联合中药离子导入干预缓解FontaineⅡ期下肢动脉硬化闭塞症(ASO)患者临床症状的影响。方法 选取河北省中医院周围血管科2020年11月—2021年10月收治的符合诊断的FontaineⅡ期ASO住院患者130例, 随机分为4组: A组(对照组)、B组(虎符铜砭刮痧组)、C组(中药离子导入组)、D组(联合组)。A、B组各31例, C、D组各34例。A组给予专科护理, B组专科护理联合虎符铜砭刮痧干预, C组专科护理联合中药离子导入干预, D组专科护理来讷河虎符铜砭刮痧和中药离子导入干预。共干预15 d, 分别于干预前和干预后测定症状量化评分及疼痛视觉模拟评分。结果 干预后4组症状评分各维度评分和疼痛评分差异有统计学意义(P＜0. 05); 与干预前相比, B、C、D组干预后症状评分各维度评分和疼痛评分均下降(P＜0. 05), 且干预后三组各评分均低于A组(P＜0. 05); 干预后D组症状评分各维度评分和疼痛评分均低于B、C组(P＜0. 05), 但B、C组干预后各评分差异无统计学意义(P＞0. 05)。结论 虎符铜砭刮痧联合中药离子导入干预可更显著地降低ASO患者症状评分, 缓解患者酸胀、麻木等症状, 并可减轻患者疼痛, 促进舒适, 为ASO患者提供更优质护理。)

Purpose – Global supply chains experienced unprecedented changes in 2020 and the relationship between domestic and global markets needed adjustments considering the long-term impacts of the changes that are unfolding around these markets. China has become the first country to announce a formal strategy – “Dual Circulation” Strategy (DCS) – to guide its self-reliant economic development in the post-COVID era. However, what exactly is the DCS and what drove China to publicize this strategy is not yet clear. This study aims to answer these questions. Design/methodology/approach – Based on an extensive review of literature and media reports, a background has been constructed that justifies the DCS as a long-overdue historic necessity. Findings – A novel definition of “Dual Circulation” is introduced. A novel construct to visualize the domestic circulation in light of international and domestic markets and international circulation has been presented. The study argues that maintaining optimum levels of consumption and saving rates is crucial to the DCS’s success. Originality/value – The study pioneers the first scientific definition of the “Dual Circulation” that will pave way for future debate on the topic. Also, it is the first time an academic study on the DCS has been executed.

To accelerate the pace of realizing carbon peaking and carbon neutrality goals, it is increasingly urgent to optimize the energy consumption of crude oil pipeline system, which is an important part of energy system. Therefore,a universal optimization model of energy consumption for swarm intelligence optimization was proposed based on the combination of pipeline simulation and penalty factor. In this way, the intelligent optimization process was realized to make the unreasonable schemes become reasonable, and the reasonable schemes to be optimal. Specifically, comparison was made for several swarm intelligence optimization algorithms that were widely applied based on RizhaoYizheng Crude Oil Pipeline System. In terms of the energy consumption optimization of crude oil pipeline system,the differential evolution algorithm was found to be capable of providing more stable and better energy consumption,thus having a better effect of energy consumption. Further, good optimization effect was obtained by applying the new optimization model of energy consumption and the differential evolution algorithm to several actual crude oil pipelines with different characteristics, including Yizheng-Changling Pipeline, Yizheng-Changling Double Pipeline and Dongying-Linyi Double Pipeline. It is indicated that the proposed optimization method is of great universality.Thus, the research results could provide technical support for the energy saving and consumption reduction of crude oil pipeline systems.

Vehicle emissions have become a significant contributor to urban air pollution. However, studies specific to city-level vehicle emission inventories are still scarce and tend to be outdated. This study introduces a methodology for developing high-resolution monthly vehicle emission inventories. We applied this methodology to Changzhou in 2022 to analyze emission characteristics and generate gridded emission data with a resolution of 0.01° × 0.01°. The results show that the total vehicle emissions of carbon monoxide (CO), volatile organic compounds (VOCs), nitrogen oxides (NOx), and fine particulate matters (PM2.5) in Changzhou are 39.69, 8.68, 18.6, and 0.56 Gg, respectively. Light-duty passenger vehicles are the main contributors to CO (74.3%) and VOCs (86.1%) emissions, while heavy-duty trucks play a significant role in NOx (50.7%) and PM2.5 (34.7%) emissions. Gasoline vehicles are mainly responsible for CO (78.6%) and VOCs (91.4%) emissions, while diesel vehicles are the primary source of NOx (81.1%) and PM2.5 (70.6%) emissions. Notably, China IV vehicles have the highest emission contribution rates (ranging from 32.5% to 44.9%). Seasonally, emissions peak in winter and are lowest in April. Spatially, emission intensity is higher in the northeast of Changzhou compared to the west and south. The methodology presented in this study offers a valuable tool for developing comprehensive city-level emission inventories, and the results provide critical insights that can inform the formulation of effective environmental policies.

In this article, we develop a compact finite difference scheme for a variable-order-time fractional-sub-diffusion equation of a fourth-order derivative term via order reduction. The proposed scheme exhibits fourth-order convergence in space and second-order convergence in time. Additionally, we provide a detailed proof for the existence and uniqueness, as well as the stability of scheme, along with a priori error estimates. Finally, we validate our theoretical results through various numerical computations.

To deeply explore the development path of intelligent construction of bridge engineering safety management under the background of intelligent construction in China’s construction industry, based on the technical characteristics of bridge engineering and the difficulties of safety management, this paper analyzes the current situation and implementation path of intelligent development of bridge engineering in China, discusses the supporting role of modern information technology on intelligent safety management of bridge engineering, and finally analyzes the future development challenges of intelligent safety management of bridge engineering. To provide a reference for the safety management of future bridge engineering projects and rapidly improve the overall safety production level of the industry.

Individual differences of learners will affect foreign language learners’ use of learning strategies. This study focuses on Chinese learners in Sri Lanka, and analyzes the correlation between their age, gender, length of time and oral language learning strategies. The results showed that there was no significant difference in the use of learning strategies among learners of different genders; with the increase of age, the frequency of overall use of strategies showed a downward trend; the frequency of use of cognitive strategies, compensation strategies and affective strategies increased with learning length while increasing, the frequency of use of meta-cognitive strategies, memory strategies and social strategies decreased with the increase of learning length.

In order to analyze the overburden failure characteristics and surface water infiltration law of coal seam mining through gully, the typical working face of coal seam mining through gully is selected as the research object. Based on the engineering geological conditions of typical working face and the physical and mechanical properties of strata, the numerical model of actual strata is established by UDEC, and the overburden failure characteristics of coal seam mining through gully are studied. The results show that the height of water-conductive fractured zone is 230-250 m by numerical calculation and 225-245 m by field measurement. By extracting mining fissures of overburden and introducing them into COMSOL, the numerical calculation model of surface water infiltration after coal seam mining is constructed, and the law of surface water infiltration to the working face along the mining fissures is analyzed. The calculation results show that when the working face is advanced to 200, 250 and 300 m, the water-conductive fractured zone connects with the surface, and the surface water infiltrates into the working face along the fracture, and the stable water quantity is 10.4, 14.3 and 19.7 m3/h respectively. Finally, taking the working face advancing to 300 m as an example, the stable water quantity of working face obtained by numerical calculation and field measurement is compared and analyzed, and the difference between the two results is small.

Present study aimed to explore the effects of intermittent cold-exposure (ICE) on culprit plaque morphology in patients with ST-segment elevation myocardial infarction (STEMI) in frigid zone.

As the most widely used gear measuring instrument, the gear measuring center can measure the individual deviations of a gear tooth flank other than the comprehensive deviations of the gear. However, gear transmission error is an important transmission performance indicator in the gear meshing process. It is an important trend of gear measuring to obtain the transmission error from individual deviations. In this study, a calculation method of gear transmission error is proposed based on the point clouds of the gear obtained by optical sensors. According to the gear meshing principle, a method is introduced to determine the contact status between the tooth flanks formed by the point clouds. According to this introduced method, the single tooth pair meshing process and the meshing process of multiple tooth pairs are analyzed to determine the gear transmission error curve. The comparison results of tooth contact analysis and gear measurement experiments verify the proposed virtual measurement method.

The Western Pacific Coasts (WPC) are the outlets of many large Asian rivers. In recent years, the interplay of climate changes and human activities has persistently altered the suspended sediment concentrations (SSC) in the WPC, triggering substantial shifts in coastal ecosystems. However, the scarcity of coastal observation stations hampered comprehensive investigations at large scales. This study employed three representative SSC retrieval models and utilized Landsat images acquired from 1990 to 2020 to estimate the SSC in the WPC with a focused endeavor to dissect the intricate spatial and temporal variability of SSC in the region. The findings revealed the following insights: (1) The outcomes derived from the three distinct SSC models consistently manifested a discernible decreasing pattern in SSC changes over the past three decades across all six major estuaries (Liao River Estuary, Yellow River Estuary, Yangtze River Estuary, Hangzhou Bay, Pearl River Estuary, and Mekong River Estuary). (2) The seasonal attributes of the six major estuaries differed, primarily due to distinct dominant influencing factors like precipitation, upstream sediment load, wind, and tides. (3) Collectively, SSC tends to be relatively higher in the Yangtze River Estuary, Hangzhou Bay, and Yellow River Estuary, while the Pearl River and Mekong River Estuaries exhibit relatively lower levels. Notably, the SSC exhibited distinct spatial traits along the coastlines of different estuaries. (4) SSC in the non-estuarine regions along the WPC, a similar significant declining trend in SSC is observed as in the estuaries, albeit the rate of decline generally appeared to be less pronounced. Furthermore, regions with faster rates of SSC reduction are typically concentrated near major estuaries in the northern part of the Coasts. The decline in estuarine SSC plays an important role in the overall decrease in SSC across the WPC. These study outcomes held substantial significance for advancing the stability and sustainable evolution of the WPC.

An enclosed karst depression, a typical natural negative terrain, has the advantage of less engineering excavation when constructing a reservoir. In this study, the enclosed karst depression and its range identification technique have been developed. What is more, the geometric parameters and spatial distribution of enclosed karst depressions in Anlong County, Guizhou Province of China, have also been analyzed. Results show that (1) the focus statistic method and local terrain contour tree model were developed to identify enclosed karst depression and its range using regular grid DEM data with 12.5 m spatial resolution, which has been applied to enclosed karst depression identification in Anlong County. (2) 7262 independent and nested depressions with an average density of 3.7/km2 were identified by using the proposed method. The effectiveness and reliability of the proposed model have been verified through comparative analysis and visual recognition comparison. (3) High-density depression areas (5.6 depressions/km2), medium-density depression areas (2.9 depressions/km2), and low-density depression areas (1.1 depressions/km2) were well classified through kernel density analysis. (4) The geometric parameters of enclosed karst depressions (area, perimeter, circularity, depth, elevation, slope, and volume) were all analyzed in the study area. In addition, an indicator called DCK (depression is caused by karstification) was proposed to evaluate the dissolution degree and karstification stage of the enclosed karst depression. Based on the DCK, we determined that around 2.7% of depressions were identified as middle-stage and suitable for reservoir construction with enough volume and good slope stability. The idea and method in this research could provide a technological support for the engineering utilization of enclosed karst depressions.

The mining of Jurassic shallow coal seam in northern Shaanxi coal base is faced with a variety of water hazards, and the traditional coal mine water hazard assessment has some limitations, so it is necessary to carry out more detailed water hazard model and zoning research in this area. Taking 3-1 coal seam of Hongliulin Coal Mine as the research object, we calculate the development height of water flowing fractured zone in coal mining, draw the contour map of development height of water flowing fractured zone, and propose the water disaster mode division method based on coal mine water disaster types by using the mutual position relationship between each aquifer and water flowing height, and divide different water disaster modes in the study area. The results show that: according to the different water filled aquifers(bodies) in the study area, the water damage types can be divided into three main water damage modes: single aquifer water damage mode, composite aquifer water damage mode and ancient Zhiluo river water damage mode. According to different water filling sources, the single aquifer water damage model can be divided into roof burnt rock water damage type and roof weathered bedrock water damage type; the composite aquifer water damage model can be divided into roof burnt rock direct-loose layer indirect water damage type, roof weathered bedrock direct-loose layer direct water damage type, roof burnt rock direct-loose layer direct water damage type and roof weathered bedrock direct-loose layer direct water damage type water damage type. The single aquifer water damage model is mainly distributed in the water flowing fracture zone, roof weathered bedrock aquifer and the area with hidden burnt rock aquifer above the mining coal seam; the composite aquifer water damage model is distributed in the water flowing fracture zone breaking through weathered bedrock aquifer, directly or indirectly affecting the loose aquifer area; the ancient Zhiluo river water damage model is distributed in the ancient Zhiluo river and the ancient river terrace. According to the analysis of the risk of water inrush under different water damage modes, the type of burning rock water damage generally does not pose a great threat to coal mining, and the risk of water inrush is small. Roof weathered bedrock water damage model, roof composite water damage model and ancient Zhiluo river water damage model are highly water-rich in some areas, and may enter the underground through the water conduction fault zone, so there is a certain risk of water inrush.

ObjectiveTo analyze the product information, food label and nutrients composition characteristics of approved foods for special medical purposes (FSMP).MethodsThe product and label information of approved FSMP products were collected from China's State Administration for Market Regulation by March 5, 2021, and the classification, ingredients characteristics and nutrients content of each product as well as the comparison with standard requirements were analyzed.ResultsThere are 58 approved FSMP products in China at present.Most labeled nutrient contents were slightly higher than the minimum and lower than the middle line of content requirements of national standard. More than 50% of the products added choline, inositol, taurine and L-carnitine, and 72.2% of complete-nutrition formula added dietary fiber.ConclusionThe approved FSMP products had basically covered all categories in China. Nevertheless, the specific complete-nutrition food and incomplete-nutrition food were inadequate which were urgent in need of research and development. The nutrients content of FSMP products were within the ideal range of GB standard.

Aged hematopoietic stem cells (HSC) are characterised by reduced regenerative potential and a loss of quiescence. Here, the authors show nicotinamide riboside treatment shrinks the age-enlarged stem cell pool and shifts aged HSC functionally, metabolically and molecularly towards the young state.

In compressed sensing theory, the most critical issue is the construction of the observation matrix. The factors that affect the image reconstruction quality include the independence between the observation matrix column vectors and the cross-correlation between the observation matrix and the sparse basis. Based on this, an optimization algorithm is proposed. The algorithm uses QR decomposition to increase the independence of the observation matrix columns, and at the same time optimizes the Gram matrix contracted using an equiangular tight frame(ETF). By updating the direction of each gradient descent, the convergence rate is accelerated to reduce The cross-correlation between the small observation matrix and the sparse basis. Simulation experiment results show that the method of optimizing the observation matrix in this paper has certain advantages in improving the quality and stability of image reconstruction under the same signal sparsity or observation times.