1. A Novel Methodology for Detecting Variations in Cell Surface Antigens Using Cell-Tearing by Optical Tweezers
- Author
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Chih-Lang Lin, Shyang-Guang Wang, Meng-Tsung Tien, Chung-Han Chiang, Yi-Chieh Lee, Patrice L. Baldeck, Chow-Shing Shin, Laboratoire de Chimie - UMR5182 (LC), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Erythrocytes ,Optical Tweezers ,Clinical Biochemistry ,C.-S. A Novel Methodology for Detecting Variations in Cell Surface Antigens Using Cell-Tearing by Optical antigenic variation ,Biomedical Engineering ,C.-L ,Chiang ,C.-H ,Antibodies ,Analytical Chemistry ,Y.-C ,Wang ,agglutination test ,Shin ,Instrumentation ,Engineering (miscellaneous) ,[PHYS]Physics [physics] ,antigenic variation ,cell surface antigens ,whole-cell-based analysis ,optical tweezers ,cell-tearing ,S.-G ,Lasers ,Lee ,General Medicine ,Lin ,Antigens, Surface ,Baldeck ,M.-T ,Tien ,P.L ,Biotechnology - Abstract
The quantitative analysis of cell surface antigens has attracted increasing attention due to the antigenic variation recognition that can facilitate early diagnoses. This paper presents a novel methodology based on the optical “cell-tearing” and the especially proposed “dilution regulations” to detect variations in cell surface antigens. The cell attaches to the corresponding antibody-coated slide surface. Then, the cell-binding firmness between a single cell and the functionalized surface is assayed by optically tearing using gradually reduced laser powers incorporated with serial antibody dilutions. Groups B and B3 of red blood cells (RBCs) were selected as the experiment subject. The results indicate that a higher dilution called for lower power to tear off the cell binding. According to the proposed relative-quantitative analysis theory, antigenic variation can be intuitively estimated by comparing the maximum allowable dilution folds. The estimation result shows good consistency with the finding in the literature. This study suggests a novel methodology for examining the variation in cell surface antigens, expected to be widely capable with potential sensor applications not only in biochemistry and biophysics, but also in the micro-/nano- engineering field.
- Published
- 2022