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胃朗格汉斯细胞组织细胞增生症临床少见。本文对1例经病理形态学、免疫组织化学检测确诊的胃朗格汉斯细胞组织细胞增生症的病例进行报道，并复习相关文献，探讨其病理形态、临床特征、诊断及鉴别诊断要点。.

日本红菇属非致死性胃肠炎型毒蕈，毒性较轻，患者预后良好。本文对2019年7月温州医科大学附属第一医院收治的一例日本红菇中毒致消化道出血患者的临床资料进行回顾性分析，患者中毒表现为典型的胃肠症状，出现严重消化道出血，最终经积极止血、护胃、补液、抗炎治疗后好转出院。.

The human health risk (HHR) assessment to dense non-aqueous phase liquids (DNAPLs) exposure has become an important part of groundwater environment management. Usually, DNAPL transport models are applied to simulate the concentration distribution of contaminant for HHR assessment. The present paper studied the influences of model uncertainties on the HHR assessment, and the metric of Incremental Lifetime Cancer Risk (ILCR) was used to quantify HHR. The impacts of permeability’s heterogeneity and the structure of DNAPL transport model (e.g., the constitutive model) on HHR assessment were evaluated based on a synthetical DNAPL transport model. The results demonstrate that, compared with the low heterogeneity, the high heterogeneity leads to lower average ILCR value at the control planes near the source zone, and higher average ILCR value at the control planes far away from the source zone. In addition, the HHR assessments would be inconsistent for the two constitutive models, i.e., Stone-Parker (S-P) and Coreyvan Genuchten (C-v) models. Compared with the HHR assessment depending on C-v model, the mean of ILCR’s probability distribution produced by S-P model is larger at the control planes near the source zone, and smaller at the control planes far away from the source zone. Moreover, based on a sandbox experiment, the impact of parameter uncertainty of DNAPL transport model on HHR assessment was evaluated by Markov chain Monte Carlo (MCMC) simulation. The results show that it is infeasible and risky to assess HHR by the specific parameters of contaminant transport model and ignoring parameter uncertainty. The HHR assessment by incorporating Bayesian uncertainty analysis could provide more flexible information. In addition, the sparse grid (SG) surrogate is an effective way to reduce computation burden caused by the larger number of model executions in the MCMC based HHR assessment.

Scientists demystify stress changes within tens of days before a mainshock and often utilize its foreshocks as an indicator. Typically, foreshocks are detected near fault zones, which may be due to the distribution of seismometers. This study investigates changes in seismicity far from mainshocks by examining tens of thousands of M≥2 quakes that were monitored by dense seismic arrays for more than 10 years in Taiwan and Japan. The quakes occurred within epicentral distances ranging from 0 to 400 km during a period of 60 d before and after the mainshocks that are utilized to exhibit common behaviors of seismicity in the spatiotemporal domain. The superimposition results show that wide areas exhibit increased seismicity associated with mainshocks occurring more than several times to areas of the fault rupture. The seismicity increase initially concentrates in the fault zones and gradually expands outward to over 50 km away from the epicenters approximately 40 d before the mainshocks. The seismicity increases more rapidly around the fault zones approximately 20 d before the mainshocks. The stressed crust triggers ground vibrations at frequencies varying from ∼5×10-4 to ∼10-3 Hz (i.e., variable frequency) along with earthquake-related stress that migrates from exterior areas to approach the fault zones. The variable frequency is determined by the observation of continuous seismic waveforms through the superimposition processes and is further supported by the resonant frequency model. These results suggest that the variable frequency of ground vibrations is a function of areas with increased seismicity leading to earthquakes.

本文对2019年2月温州医科大学附属第一医院收治的2例急性重症氨基甲酸酯类农药中毒患者的临床资料进行分析。患者病情较重，出现呼吸衰竭及昏迷，最终经治疗好转出院。对于氨基甲酸酯类农药中毒，主要予抗胆碱能作用及脏器支持治疗。.

It is reported that circular RNA plays an important role in various cancers in recent years. However, there is less investigation reported in lung adenocarcinoma (LUAD) about circRNA. This study aims to explore the role and molecular mechanism of circle RNA FOXP1 in LUAD procession.The levels of circFOXP1 and miR-185-5p in LUAD cell lines and LUAD cancer samples were examined by RT-PCR. The functions of circFOXP1 and miR-185-5p at LUAD cells were detected by cell transfection of the overexpression or repression. The A549 and H1299 cell proliferation were detected by MTT assay and colony formation assay. And the cell apoptosis was detected by TUNEL assay. The expression levels WNT1 were measured by Western blot in A549 and H1299 cells. Furthermore, the luciferase assay detected the direct interaction between circFOXP1 and miR-185-5p or miR-185-5p and WNT1.The circFOXP1 expression was increased in LUAD patients and LUAD cell lines. The downregulation of circFOXP1 significantly repressed LUAD cell proliferation and promoted cell apoptosis. Moreover, the luciferase assay results confirmed that circFOXP1 directly interacted with miR-185-5p. Overexpression of miR-185-5p could reverse the effect of circFOXP1 in LUAD cell. Besides, the luciferase results showed that miR-185-5p directly interacted with WNT1. miR-185-5p overexpression inhibited the WNT1 expression, while circFOXP1 repression decreased the WNT1 level in LUAD cell lines. The downregulating WNT1 could reverse the effects of miR-185-5p inhibition in LUAD cell lines. Furthermore, WNT1 was significantly upregulated in LUAD cancer tissues. In addition, circFOXP1 level was negatively correlated with miR-185-5p expression and positively correlated with WNT1 expression in LUAD cancer tissues.These data suggested that circFOXP1 promoted cell proliferation and repressed cell apoptosis in LUAD by regulating miR-185-5p/WNT1 signaling pathway. It provides a novel potential therapeutic agent for the treatment of LUAD.

Heart failure (HF) is the loss of myocardial structure and function caused by various congenital or acquired heart diseases. This study explored the new target of treatment of HF by investigating the effect of Kallistatin (KS) on inflammation and apoptosis of myocardial tissue in HF rats.We used doxorubicin to induce rat HF, and determined the success rate of modeling by detecting changes in rat heart weight and body weight, cardiac function and histology. We used two different doses (1 mg/kg, 2 mg/kg) of KS intraperitoneally injected rats and detected changes in inflammation and apoptosis of rat myocardial tissue by enzyme-linked immunosorbent assay (ELISA), Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and immunohistochemical staining. Changes in the expression of sirt1 were also detected. In addition, we cultured rat myocardial cell line, H9c2 cells, and used siRNA-sirt1 to inhibit sirt1 in H9c2 cells to clarify the mechanism of KS regulating myocardial cells.The body weight of HF rats treated with KS decreased while the heart weight increased. KS has also been found to reduce the concentration of brain natriuretic polypeptide (BNP) in rat serum. The results of echocardiography showed that KS effectively relieved the cardiac function of HF rats. Inflammatory factors (interleukin (IL)-1β, IL-6, IL-8 and tumor necrosis factor (TNF)-α) and pro-apoptotic molecules (caspase3/9 and Bax) in the serum and myocardial tissue of rats treated with KS were also significantly reduced. The inhibition of sirt1 in H9c2 cells significantly reduced the anti-apoptotic effect of KS on H9c2 cells.KS reduces the inflammation and apoptosis of myocardial tissue in HF rats by promoting the expression of sirt1, thereby alleviating HF-induced myocardial injury.

To explore the effects of micro ribonucleic acid (miR)-32 on the proliferation and apoptosis of myeloma cells, and to verify whether it exerts its function by targeting phosphatase and tensin homolog deleted on chromosome ten (PTEN).The differentially expressed miRNAs were screened in healthy people and myeloma patients. The myeloma U266 cells transfected with negative control (NC) were used as control group, those transfected with miR-32 inhibitor as transfection group, and those transfected with miR-32 inhibitor and treated with PTEN inhibitor SF1670 as the transfection + inhibitor group. Then, the cell proliferation and apoptosis in each group were detected using the 5-Ethynyl-2'-deoxyuridine (EdU) kit and terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay, respectively. Finally, the expressions of apoptosis-related proteins B-cell lymphoma-2 (Bcl-2), Bcl-2 homologous antagonist/killer (Bak), caspase-9, and survivin were detected.The expressions of some miRNAs and genes in myeloma patients were significantly different from those in healthy people. In myeloma patients, miR-32, miR-126, miR-123, and miR-183 were significantly highly expressed, while miR-5, miR-76, and miR-50 were remarkably lowly expressed. After myeloma U266 cells were transfected with the miR-32 inhibitor, the expression of miR-32 markedly declined. In addition, the mRNA expression of PTEN in myeloma cells rose after transfection with the miR-32 inhibitor, and declined after addition of the PTEN inhibitor SF1670, which were consistent with the results of Western blotting. Besides, the proliferation ability of myeloma cells was evidently weakened after transfection with the miR-32 inhibitor, while it was restored to a certain extent after addition of the PTEN inhibitor SF1670. Moreover, the number of apoptotic myeloma cells was remarkably larger after transfection with the miR-32 inhibitor, while it was remarkably smaller after addition of the PTEN inhibitor SF1670. The expressions of pro-apoptotic proteins Bak and caspase-9 in myeloma cells were significantly increased after transfection with the miR-32 inhibitor (p0.05), and significantly decreased after addition of the PTEN inhibitor SF1670, while the expressions of anti-apoptotic proteins Bcl-2 and survivin were opposite to those of Bak and caspase-9.MiR-32 targeting PTEN will have certain effects on the proliferation and apoptosis of myeloma cells.

Selective electron beam melting (SEBM) is an established layer additive manufacturing or production process for small-to-medium–sized components of Ti-6Al-4V. Current literature data on SEBM of Ti-6Al-4V are, however, based principally on thin-section (

Diabetic retinopathy (DR) is one of the most important etiologies of diabetic blindness. The vascular endothelial growth factor (VEGF) is thought to mediate pathophysiological changes of DR. Anti-VEGF therapy for DRdevelops rapidly. However, there are some misunderstandings about its clinical application, and the strategy is not clear. Sometimes its therapeutic effects are even exaggerated. In this article, by discussing whether anti-VEGF therapy can reverse the course of DR disease, promote the absorption of vitreous hemorrhage, and replace fundus laser therapy, the application strategy of anti-VEGF therapy for DR is proposed. At present, anti-VEGF therapy cannot completely replace retinal photocoagulation therapy, and its clinical application value should not be exaggerated. More innovative drugs and therapies are expected to provide new ways forthe treatment of DR while preserving visual function.糖尿病性视网膜病变（DR）是糖尿病致盲最重要的病因，血管内皮生长因子（VEGF）被认为是介导DR病理生理改变的重要因素，由此衍生出的抗VEGF疗法在DR治疗领域迅速发展，但同时临床应用也出现一些误区，对其应用策略不清晰，甚至夸大了其治疗作用。本文通过探讨抗VEGF疗法能否逆转DR的病程、能否促进DR玻璃体积血吸收、能否取代眼底激光治疗，提出抗VEGF疗法在DR治疗中的应用策略，指出现阶段在抗VEGF疗法不能完全取代眼底激光治疗的前提下，尚不应夸大其临床应用价值。在未来，期待有更多创新药物和疗法为治疗DR并同时保护视功能提供新的途径。

The millions of tons of textile waste were produced every year. Most of them were made from polyester/cotton blended fabrics. The key technologies for recycling the waste blended fabrics were how to separate the blended components and then use the materials form the waste. Based on this, this research aimed to develop the technology of sustainable recycling of cotton fiber and polyester. The waste polyester/cotton blended fabric was first to cut into small pieces, then treated it with acid, and then used a grinder to crush it to separate the polyester from the cotton fiber. After the separation process, the cotton fiber was powder and the polyester fiber was fibrous mass. The components of the separated cotton samples were studied by Fourier transform infrared spectroscopy. The crystallinity of separated cotton fiber was analyzed by X-ray diffraction. The morphology of the separated cotton fiber and polyester were analyzed by scanning electron microscope. The particle size and potential of the cotton fiber after final treatment were analyzed by using the nanoparticle size and zeta potential analyzer. The results showed that the waste polyester/cotton blended fabric was successfully separated and the separated cotton fiber was successfully prepared into cotton nanocellulose for future use.

In order to study the interaction mechanism of the pipe network, the compressor and the control system in the process of the anti-surge regulation, the simulation model of the overall Closed Cycle System (CCS) is established in this paper. By comparing the simulation results with the target values at the Design Operation and the Maximum Mach Operation, the calculation deviation is less than 1%, which verifies the accuracy of the model. At the Maximum Mach Operation, the analysis of the CCS during the quasi steady process and the dynamic process is carried out respectively. According to the result of quasi steady state analysis, the effectiveness of the designed anti-surge circuit during the normal operating process of the CCS is verified. By opening the Anti-Surge Valve, additional 38.5% surge margin is provided. And the surge margin at the simulation process would be slightly higher than the design value for the variation of the inlet condition from the different operations. Through the dynamic analysis, the changes of the different performance parameters are studied in the process of sudden blockage in the pipe network, and during this process the compressor surge could be induced easily due to the sharp decline of the inlet pressure. To ensure the safety of the compressor during the acceleration and deceleration process, the moving trajectory of the operation point on the Compressor Characteristic Map should be studied by considering the effect of the resistance characteristics of the CCS pipe network. In addition, the anti-surge system should have a high response frequency to deal with the sudden blockage.

Achieving a high surface finish is a major challenge for most current metal additive manufacturing processes. We report the first quantitative study of the influence of as-built surface conditions on the tensile properties of Ti-6Al-4V produced by selective electron beam melting (SEBM) in order to better understand the SEBM process. Tensile ductility was doubled along with noticeable improvements in tensile strengths after surface modification of the SEBM-fabricated Ti-6Al-4V by chemical etching. The fracture surfaces of tensile specimens with different surface conditions were characterised and correlated with the tensile properties obtained. The removal of a 650-μm-thick surface layer by chemical etching was shown to be necessary to eliminate the detrimental influence of surface defects on mechanical properties. The experimental results and analyses underline the necessity to modify the surfaces of SEBM-fabricated components for structural applications, particularly for those components which contain complex internal concave and convex surfaces and channels.

To detect the change in miRNA-210 expression of cardiomyocytes under hypoxia/reoxygenation status. Also, the effect of miR-210 on the apoptosis of cardiomyocytes induced by oxygen-glucose deprivation/reperfusion (OGD/R) and its mechanism through establishing the OGD/R injury model of primary cardiomyocytes in this experiment were investigated.The cell model of OGD/R injury was established. The cell apoptosis in each group was detected by methyl thiazolyl tetrazolium (MTT) assay and detection of Caspase-3 activity. The change in miR-210 expression in each group was detected by Real-time fluorescence quantitative polymerase chain reaction (PCR). The high-expression and low-expression miR-210 models were established through the transient transfection of miR-210 mimic and inhibitor to detect the relevant indexes of cell apoptosis. At the same time, changes in mRNA and protein expressions of E2F3 were detected by RT-PCR and Western blotting, respectively. The E2F3 overexpression vector was constructed, and the overexpression vector plasmid and miR-210 mimic were jointly transfected into the cells to detect the relevant indexes of cell apoptosis.After OGD/R treatment, the activity of Caspase-3 was increased, the survival of cardiomyocytes was significantly inhibited and the expression level of miR-210 was up-regulated in OGD/R injury. Transfection of miR-210 mimic for miR-210 overexpression could alleviate the OGD/R-induced cardiomyocyte injury, while the decrease of miR-210 expression could aggravate the apoptosis of cardiomyocytes. In addition, the high expression of miR-210 could inhibit the protein expression of E2F3, and co-transfection of E2F3 plasmid and miR-210 mimic could reverse the inhibiting effect of miR-210 on the apoptosis of cardiomyocytes.We confirmed that miR-210 can inhibit the OGD/R-induced apoptosis of cardiomyocytes, and miR-210, as an upstream factor, plays a protective role in cardiomyocytes through directly inhibiting the protein expression of its target gene E2F3.

Lowering the cost of feedstock powder has been a major issue for wider applications of additive manufacturing (AM) of titanium (Ti) and its alloys. A novel and inexpensive Ti sponge material was selected as a precursor and processed using a CSIRO proprietary powder manipulation technology (PMT). The manipulated powder was characterized in terms of the particle size distribution (PSD), roundness, flowability in the Hall Funnel flowmeter, static angle of repose (AOR), apparent density and tap density. In addition, a universal powder bed (UPB) system was used to characterize the manipulated powder behavior after raking. Two benchmark powders, virgin Arcam Ti-6Al-4V powder and used Arcam Ti-6Al-4V powder, were assessed for a comparison. PMT processing of the Ti powder precursor produced near spherically shaped Ti powder in the size range of 75–106 µm, which performed very similarly to the used Arcam powder in the UPB system. The CSIRO PMT offers a cost-effective manipulation process to produce Ti powder promising for AM applications, while the UPB system allows a quick assessment of the powder spreading behavior in AM processes.

Multiferroic materials, like MnWO4, exhibit improper ferroelectricity driven by an inversion symmetry breaking magnetic order and strong spin lattice interactions. We show that the three magnetic phases in MnWO4, including one ferroelectric phase, can be controlled by substitution of copper for manganese and by application of external magnetic fields. Upon increasing Cu substitution, the paraelectric low-temperature phase is replaced by the ferroelectric phase which becomes the ground state at higher doping levels. We conclude that a mixed (para-/ferroelectric) ground state exists below 10% of Cu substitution.

Sexual dimorphism in size or shape is widespread in the animal world. Many studies have explored this topic, but few have focused on Asian salamanders. In this study, we analyzed morphometric data of the Wushan salamander Liua shihi, an endemic Chinese salamander, to examine sexual dimorphism in size and shape. We evaluated data sets that included 17 morphometric characteristics of 61 females and 55 males using univariate and multivariate methods. Results show that sexual dimorphism in this species includes not only body size (males have a longer snout-vent length than females), but also shape (females have a longer space between axilla and groin and head size than males, while males have greater limb size). This article discusses the evolution of intersexual dimorphism according to models of ecology, fecundity and sexual selection. We propose that sexual dimorphism of body size can be attributed to sexual selection and local climates, that AGS may contribute to fecundity selection, that head size...