Search

Your search keyword '"Y. Ookuma"' showing total 20 results
Start Over Author "Y. Ookuma"
20 results on '"Y. Ookuma"'

2. P1.14-03 Phase II Trial of Amrubicin and Cisplatin Chemotherapy for Invasive Thymoma: WJOG5509L

Author

Y. Shikraisi, Akira Ono, S. Mituoka, Kazuhiko Nakagawa, Koichi Azuma, Kiyonobu Ueno, Makoto Nishio, Katsuhiro Okuda, Yasutaka Chiba, Kenji Sugio, K. Kudo, Yasuo Iwamoto, K. Akiyoshi, T. Iriki, Yuichi Ishikawa, Y. Ookuma, Kazumi Nishino, Nobuyuki Yamamoto, and Isamu Okamoto

Subjects
Pulmonary and Respiratory Medicine, Cisplatin, Oncology, medicine.medical_specialty, Chemotherapy, business.industry, medicine.medical_treatment, Invasive thymoma, Internal medicine, medicine, business, Amrubicin, medicine.drug
Published
2018
Full Text
View/download PDF

3. Assessment of lectin binding for prognosis in endometrial carcinoma

Author

Y Ookuma, Hajime Sugimori, Tsuyoshi Iwasaka, and Hachisuga T

Subjects
Peanut agglutinin, Pathology, medicine.medical_specialty, Adenocarcinoma, Endometrium, Pathology and Forensic Medicine, Immunoenzyme Techniques, Peanut Agglutinin, Agglutinin, Lectins, medicine, Carcinoma, Humans, Neoplasm Staging, biology, Lectin, Prognosis, medicine.disease, Survival Analysis, Endometrial Neoplasms, Staining, medicine.anatomical_structure, cardiovascular system, biology.protein, Immunohistochemistry, Female, Plant Lectins, Neuraminidase
Abstract

Summary The binding of Ulex europeus agglutinin-I (UEA-I) and Peanut agglutinin (PNA) was studied in 56 endometrial carcinomas. The positive rates of UEA-I and PNA were 66%. Following neuraminidase treatment, the PNA positive rate increased from 66% to 98% and most tumors stained abu0dantly. UEA-I staining was unaffected. Using a combined grading system based on architectural and nuclear abnormalities, the PNA positive rates of grade 1, grade 2 and grade 3 tumors were 78%, 70% and 36%, respectively. There was no correlation between UEA-I binding and the grading systems. UEA-I binding correlated significantly with the presence of myometrial invasion and vessel permeation. Metastatic endometrial carcinomas showed a high affinity of tumor cells for UEA-I. Tissues that stained with UEA-I were those from patients with the worst prognosis. PNA and UEA-I reactivities relate to the biologic activity of endometrial carcinomas. UEA-I can serve as a prognostic marker for patients with endometrial carcinoma.

Published
1994
Full Text
View/download PDF

4. Proposal of using IH cooking heater technology for energy-saving home appliances

Author

S. Murakami, Y. Ookuma, and T. Noguchi

Subjects
Energy conservation, Engineering, Architectural engineering, Waste management, business.industry, Energy (esotericism), Mindset, Electricity, Electric power, business, Ecological awareness
Abstract

The Research Laboratory of Kyushu Electric Power promotes development of various technologies for a "comfortable life with Eco & Web". In order to propose new electricity usage valuing the mindset of customers and even new lifestyles for the era of energy saving and high ecological awareness, such technologies are adopted in our "Intelligent House". This paper deals with the development of an innovative energy-saving iron using the IH cooking heater technology.

Published
2010
Full Text
View/download PDF

5. A novel uwb impulse-radio transmitter with all-digitally-controlled pulse generator

Author

Masayuki Miyazaki, Y. Ookuma, Takayasu Norimatsu, Ken Sakamura, M. Hayakawa, Ryosuke Fujiwara, Masaru Kokubo, Noboru Koshizuka, and Satoru Kobayashi

Subjects
Engineering, business.industry, Pulse generator, Transmitter, Electrical engineering, Data_CODINGANDINFORMATIONTHEORY, Transmitter power output, Radio frequency power transmission, Power (physics), Pulse (physics), CMOS, Hardware_GENERAL, Low-power electronics, Hardware_INTEGRATEDCIRCUITS, Electronic engineering, business
Abstract

A novel transmitter for ultra-wideband (UWB) impulse radio was developed. The proposed architecture realizes low power, low complexity, and generation of a highly accurate pulse. The phase and amplitude of a pulse are controlled separately and digitally to generate a highly accurate pulse. This control method also contributes to the transmitter's low power and eliminates the need for a filter. The transmitter was fabricated by a 0.18-/spl mu/m bulk CMOS process. The core chip size is only 0.40 mm/sup 2/. Measurement of this transmitter found that the FCC spectrum mask is satisfied and average power dissipation is 29.7 mW under a supply voltage of 2.2 V. These results show that the developed UWB transmitter can generate an accurate pulse with low power and low complexity.

Published
2005
Full Text
View/download PDF

6. Proposal of transmitter architecture for mobile terminals employing EER power amplifier

Author

R. Fujiwara, Y. Ookuma, May Suzuki, Satoshi Tanaka, T. Yamawaki, and T. Tanoue

Subjects
Mobile radio, Reduction (complexity), Engineering, business.industry, Code division multiple access, Amplifier, Transmitter, Electrical engineering, Electronic engineering, business, Electrical efficiency, IEEE 802.11a-1999, Intermodulation
Abstract

New transmitter architecture employing EER (envelope elimination and restoration) method is proposed for mobile terminals. The effectiveness of the proposed architecture is evaluated by power efficiency estimation using theoretical models. The results showed its potential to gain three times higher average power efficiency as compared to that of conventional linear amplification architecture in WLAN (IEEE 802.11a) system. It accounts for 40% reduction in power dissipation. Intermodulation distortion of EER method is also evaluated. System parameters are fitted to the specifications of W-CDMA and WLAN.

Published
2004
Full Text
View/download PDF

7. A 1.2 Gbps SOI-BiCMOS write driver for hard disk drives

Author

M. Yoshinaga, H. Yoshizawa, K. Irikura, Y. Kobayashi, Y. Ookuma, and K. Maio

Subjects
Engineering, Fall time, Preamplifier, business.industry, Rise time, Impedance matching, Electronic engineering, BiCMOS, business, Data rate units, Voltage, Power (physics)
Abstract

This paper describes a voltage-type write driver for a +5/-5 V preamplifier. A good rise/fall time and smooth settling are achieved with full or partial impedance matching by maximizing the driving voltage up to the power source. Designed in 0.35 /spl mu/m SOI-BiCMOS, it has demonstrated a rise time as short as 0.34 ns with a peak-to-peak write current of more than 150 mA at a data transfer rate of 1.2 Gbps.

Published
2003
Full Text
View/download PDF

8. SRPX2 is a Novel Chondroitin Sulfate Proteoglycan that is Overexpressed in Gastrointestinal Cancer

Author

T. Hirashima, Y. Omuro, C. Kondo, T. Kanematsu, K. Muraki, Po-Chuan Wang, K. Ishiguro, Young-Ae Park, C.-Y. Lu, C.-C. Liao, H. Tei, H. Takeyama, M. Toishi, A. D. Abdullah, M. Terada, K. Yamamoto, N. Yamamoto, K. Fujii, M. Sugimoto, H. Kakizaki, K. Shinozaki, Y. Okada, Yoko Inaguma, S. Shimizu, Shigeki Ito, H. Y. Lim, N. Nogami, N. Awata, M. Nishioka, H. Ueoka, Tomoya Ishii, Y. Ahn, Kazumichi Kawakubo, Y. Aoyagi, C. Nishijima, R. Kameda, A. Okamoto, Y. Yamashita-Kashima, H. Suzuki, K. Yamao, A. Yonemori, H. Fukuda, H. Katayama, K. Honoki, T. Nomura, Y. Tono, T. Shimoyama, J. Nagano, H. Miyamoto, Y. Takeda, M. Fukutake, N. Katsumata, S. Fujita, K. Fujimoto-Ouchi, D. Tamura, H. Obaishi, S. Mitsunaga, J.-H. Baek, Yuichiro Tada, K. Uno, S. Oura, M. Nakamura, Y. Imanura, Atsushi Kumanogoh, M. Manabe, Kaoru Tanaka, T. Yokota, K. Saito, K. Tamura, Yukihiko Fujii, T. Lim, Toshihiko Tomita, C. Seki, Masafumi Taniwaki, Tomohide Sugiyama, N. Kunami, T. Yoshino, Y. Takeoka, T. Yoshikawa, Won-Suk Lee, M. Hattori, H. Yasui, T. Motoya, T. Nishizaki, N. Kouge, E. Sato, S.H. Park, J.H. Hong, N. Mori, M. Tajika, K. Yasuda, Mika Nakamae, Kazuya Fukuoka, T. Shimomura, A. Suzuki, M. Arima, Hideo Koh, S. Tokunaga, N. Miyamoto, Masao Nakata, T. Ueda, Hideharu Kimura, H. Nakano, Kimikazu Yakushijin, M. Hayashi, K. Ishitani, K. Yoshida, T. Takeuchi, Shohei Yokota, K. Hirano, N. Horikawa, S. Bandoh, G. Naka, Y. Seki, M. A. De Velasco, F. Tanikawa, S. Hirano, S. Ohkawa, S. Kadowaki, M. Sakurai, R. Kaji, J.-I. Lee, K. Kitahara, K. Nihei, T. Sumi, Meiki Fukuda, S. Park, K. Nosaka, T. Maeda, O. Morimura, G. Sano, H.-L. Wu, Haruhiko Hirata, Mizuki Aimoto, Y. Igeta, K. Itoh, Y. Ikari, Kentaro Iwanaga, K. Itatsu, Akira Ueda, C. Oabata, H. Fujiwara, T. W. Kim, K. Misu, H. Mikayama, K. Morise, K. Nagata, M. Sato, Takashi Kijima, Kazuo Kasahara, Takahiro Mori, N. Mizuno, Y. Fujitani, Abdul Aziz Baba, K. Takashima, Kazuhide Higuchi, J.-C. Jo, G. Tamaki, S. Magoshi, R. Watanabe, A. Abe, M. Iino, H. Goto, Junji Tsurutani, Y. Katashiba, K. Kato, K. Hosono, L. Y. Kwan, Y. Okabe, N. Takeuchi, Chih-Hsin Tang, I. Kawase, Takayuki Kii, D. Kishino, K. Matsuura, K. Isobe, K. Monden, H. Udagawa, K. Kim, M. Tada, Kazuyoshi Yanagihara, Cheryn Song, T. Terui, Yasuhito Fujisaka, I. Yamaguchi, Hirokazu Fukui, K. Naito, T. Suzumura, H. A. Jung, N. Ureshino, Wataru Okamoto, H. Miyawaki, N. Nakamura, T. Tsukazaki, K. Furuta, K. Matsuda, S. J. Lee, Y. Ishiura, J.-L. Lee, Y. Kato, Shinichiro Hayashi, Y. Horita, J. H. Kim, Y. Tsutsumi, M. Inaoki, K.-P. Kim, Y. Ishigatsubo, T. Mikawa, M. Yamane, A. Husin, Yasufumi Takeshita, S. Kobayashi, N. Kubo, N. Hosono, Yeong-Shiau Pu, M. Ando, Keita Kudo, Hitoshi Nishitani, M. Mori, H. Daga, T. Fukuda, A. Nakaya, N. Fuse, I. Miki, W. Yamamoto, M. Fukushima, T. Ikezoe, H. Ueno, J.-H. Ahn, T. Matsumoto, A. Kuwahara, T. Ogura, N. Hirai, S. Mizuta, A. Ochiai, N. Masumori, S. Kim, Y. Ohki, Yoshinori Imamura, T. Tamaki, K. Nishino, Y. Aoyama, T. Ogawa, T. Koyama, M. Morise, K. Kawada, T. Masaki, Keishi Yamashita, S. Yamamoto, K. Tanimoto, M. Hori, Atsuo Okamura, Masataka Ikeda, K. Oishi, H. Hashimoto, Y. Ohe, M. Yasui, Y. Akatsuka, F. Imamura, Y. Hirayama, Ho Young Kim, S. Kishi, M. Jung, Y. Inukai, K. Miwa, S.-H. Nam, T. Hishima, T. Okusaka, Y. Horiuchi, A. Ioka, W. Fukushima, M. Yamauchi, N. Hokamura, K. Hirata, Y. Katou, K. Tada, K. Suzuki, K. Teramoto, Syusai Yamada, M. Iikura, Takeo Shimasaki, Y. Inoue, K. Kawahara, T. Kitani, H. Sawai, T. Terashima, K. Honda, Hitomi Umeguchi, Masataka Okamoto, M. Kita, Y. Yatabe, Y.-M. Cho, Sojiro Kusumoto, K. Hokkoku, Takaaki Sasaki, Masayuki Hino, M. Omi, H. Tanaka, S. Kawazoe, M. Sakai, H. Tsuchihashi, Kazushi Endo, R. Mauchi, K. Ohashi, H. Takasaki, N. Naganobu, K. Aoe, S.Y. Oh, C. Honma, Takahiro Miyamoto, K. Yamazaki, M. Fujii, T. Fujisawa, S. Morikawa, T. Yamauchi, Masayoshi Kobune, K. Kuwano, T. Onikubo, M. Kuyama, M. Asayama, T. Kozuki, M. Kanie, Masahiko Shibuya, Y. Yamamoto, N. Morishita, Y. Yoshii, Toru Mukohara, K. Izumi, Y.S. Park, N.-R. Lee, Y. Horio, K. Nakamura, M. Matsuda, K. Sugino, S.H. Lee, S. Ueno, Tsutomu Sato, Y. Hasumi, H. Yamamoto, T. Karasuno, Yong Chan Ahn, M. Kitamura, Y. Namba, K. Karasawa, S. Hayasi, K. Hashimoto, Y. Ozaki, Takayuki Takahama, A. Todaka, M. Inoue, S. Boku, A. Ohtsu, Tadashi Matsunaga, K. Togitani, H.-H. Wu, Hirofumi Kogure, H. Kitamura, T. Matsuzaki, M. Gouchi, Hyun-Jin Kim, T. Shiroyama, K. Okada, Y. Terasaki, K. Park, H. Katou, N. Kobayashi, D. Mohri, Y. Hasegawa, T. Yoshimasu, Masahiro Tabata, S. Hijioka, Y.-Y. Chen, Shinji Nakao, M. Kodaira, Akihiko Gemma, T. Yoshida, Hiroya Takiuchi, Masaki Fujimura, A. Shimoda, Hiroyuki Isayama, K. Ohta, T.-L. Chen, T. Maruyama, K. Maruyama, K.-W. Lee, Takashi Hirose, Y. Fujita, H. Kato, Maya Watanabe, S. Iwasa, H. Okuyama, Cherry Wu, A. Hata, K. Myo, M. Takase, Y. Urasaki, K. Shingu, Shingo Nishikawa, M. Tsuzuki, I. Hoshi, T. Maruo, Hiroki Yoshita, Hirohisa Nakamae, Shigeru Hatabe, Hideko Ikeda, Hayato Koba, Y. Hata, S. Matsushima, M. Yunokawa, S. Tamaru, J. S. Ahn, T. Funakoshi, S.-J. Jang, S. Kageyama, K. Nakagawa, H. Nishimori, Eizaburo Sueoka, K. Hashidume, S. H. Hong, Atsushi Kawaguchi, Tomomi Nakamura, H. Kaneko, A. Seki, K.-L. Tan, T. Ichimura, Y. Matsuda, M. Nezu, M. Kudo, H. Fujii, K. Shibata, S. J. Sym, K. Takeuchi, Chiharu Tabata, M. Takeshita, Y. Ueda, A. Nakayama, N. Nishiyama, Sang We Kim, Y. S. Kim, H. Suzushima, S. Soma, K. Miura, H. Gonda, D. Gomi, A. Mogi, K. Ishizuka, T. Mizutani, Y. Yamada, A. Sato, G. Kaneko, T. Samejima, R. Shimabukuro, Masahide Fujita, K. Horie, R. Ohhashi, T. Wakasa, H. Nomura, K. Sato, T. Hamaguchi, S. Horiguchi, M. Ootsuka, S. Kawabata, Y. Okamoto, A. Yoshida, H. Takeda, M. Sugiyama, Y. S. Hong, Y. Yanagita, Yasushi Ichikawa, K. Tomii, T. Enokida, Tzyh-Chyuan Hour, Y. Takeyama, Y. Matsuura, Y. Kakehi, S. Kanazawa, S. Kimura, T. Yamada-Murano, D. Abe, Nagio Takigawa, T. Yana, A. Ogino, R. Sakai, S. Watanabe, K. A. Kwon, Y. Nakai, O. Watanabe, Naokatsu Nakada, Masanori Toyoda, H. Inomata, R. Sekine, J. S. Lee, T. Shukuya, O. Ishiko, Y. Ikeda, K. Nakase, S. Kuzu, H. Mukai, K. Ozaki, R. Koyama, Takashi Nakano, K. Hashizume, E. Noguchi, N. Hida, Y. Takamatsu, Tomoko Yamagishi, H. Agatsuma, S. Miyamoto, D.H. Lee, H. Kunimoto, H. Ogino, T. Miya, Naoki Sasahira, A. Yamane, T. Takami, N. Imai, Y. Fukui, Tae Min Kim, T. Kita, Jiro Watari, H. Kawabata, N. Motohashi, K. Aomatsu, T. Obayashi, H. Hayashi, S.-H. Li, S. Sakata, H. Okada, K. Masa, T. Iwata, H. Yoshida, Tokuzo Arao, R. Hassan, H. Imaoka, M. Kobayashi, H. Iwasaki, K. Nomura, H. Harada, T. Watanabe, K. Kaneko, H. Nakagawa, K. Sakamoto, A. Hiasa, Katsuyuki Hotta, Nobuhiko Emi, S. Maruyama, M. Yonemura, H. Tsurumi, Takuhiro Yamaguchi, M. Nagata, T. Nakai, Motoki Yoshida, S. Motomura, A. Sakai, H. Inoue, Toshimitsu Yamaoka, T. Morikita, S. Hirokawa, Hideaki Ijichi, Namiki Masayuki, Meiko Nishimura, Y. Ishii, A. Shimatani, Jong-Hyeok Kim, M. Ujihara, Yuko Kanbayashi, Y. Nakashima, T. Hosoda, K. Sanada, S. Kondo, Y. Honma, S. Sakamoto, H. Kubo, M. Kondo, F. Nomura, M. Hashizume, T. Shiraishi, B.-S. Kim, T. Kouno, T. Maki, H. Akaike, Z. Saito, Junya Fukuoka, T. Ohnishi, C. H. Maeng, M. Wada, Jong-Mu Sun, C. Morizane, Y. Matsumoto, K. Migita, Y. Okamura, Sun Young Rha, Hiroyoshi Ichihara, J. Kato, N. Yoshimura, W.-J. Wu, N. Wada, M. Yoshihara, K. Hamai, Kazuhiko Koike, Woo Kyun Bae, Y. Maeda, S. Mimura, Y. Sakai, H. Wakasugi, H. Nishimoto, M. Nagano, K. Taira, I. Park, T. Inokuma, Katsuhiko Shimizu, Y. Nakahara, S. Okamura, K. Ogawa, F. Saito, Y. Miura, Hyo Jin Lee, K. Fujita, K. Takagi, T. Shiina, Charny Park, Shin Kuwakado, N. Moto, Y.-C. Chiu, S. Saji, T. Araya, J. Takeshita, H. Iwase, Naoe Goto, H. Murakami, T. Hayashi, K. Otsuka, Rishu Takimoto, H. Nakahama, C.-C. Shih, Naoko Aragane, S. Hamauchi, H. K. Ahn, N. Tomita, N. Chyayahara, T. Hida, K. Watanabe, Y. Kokubo, N. Katusmata, L. K. Chi, M. Okumura, T. Kusakabe, S. Homma, H. Nakagomi, Hiroo Katsuya, D. B. Shin, Naoko Chayahara, F. Fukuta, Kazutoshi Shibuya, Ayumu Hosokawa, F. Ota, R. Yoshino, M. Goto, Y. Shibata, J. E. Kim, H. Watanabe, K. Mandai, T. Shimamura, S. Inoue, M. Fujimoto, S. Mitsuoka, Kunio Okamoto, M.-J. Kim, E. Chung, H. Moriwaki, Y. Misumi, S. Ogawa, K. C. Lee, J.-O. Lee, H. Hirosawa, Yoshiki Terada, A. Kinoshita, J. Hong, Y. J. Kim, A. Kido, M. Kijima, Y. Shiota, H. Hayase, A. Sekikawa, M. Ahn, K. Komuta, M. Sasaki, T. Murakami, M. Okuda, N. Matsubara, R. Saitou, R. Nakamura, K. Masuo, Kazuko Matsumoto, K. Mouri, Y. Ookuma, Kazutoshi Komiya, K. Sakai, N. Yogo, Takahiko Nakane, M. Mukai, Isao Tachibana, Shiro Kimbara, Kentaro Okuda, T. Fujisaki, S.-J. Chuang, Y. Niwa, H. Oda, Y. Nishida, T. Ando, Yuichi Ando, J. Tong, C. Shimizu, J. Choi, Satoshi Iyama, H. Imai, K. H. Park, T. Misao, Yohei Funakoshi, Chang-Sik Yu, Tadashi Kimura, J. Hori, M. Itoh, S. Ebihara, S.-H. Gan, T. Yano, H. Okamoto, E. Fukutani, U. Tateishi, T. Ishihara, Takuro Yoshimura, T. Shinkai, A. Yokoyama, T. Kikuchi, Y. Yamashita, K. Hagiwara, Y. Noda, Y. Oyama, K. Okuno, Naomi Kiyota, K. Yonemori, K. Kuramoto, T. Shimoi, H. Hong, Ryuya Yamanaka, E. Matsuki, O. Kondo, H. Gondou, Yusuke Nakamura, M.-J. Ahn, Yoshiki Hayashi, Shiro Koh, S. Kosaka, Masahiro Gotoh, S. Mizuno, H. Nakamura, S. Okazaki, E. Ichiki, M. Ishizu, K. Ishikawa, Hiroyasu Kaneda, R. Yamamura, Tomonobu Koizumi, R. Ankathil, T. Takahashi, S. Nakatsuka, A. Kamuro, M. Ueno, T. Eguchi, S. Hirai, G. Saito, S. Kudoh, Masanao Nakashima, N. Okamoto, K. Akiyoshi, Hironobu Minami, K. Kubota, K. Okafuji, M. Aoe, T. Ito, K. Nishimura, S. Ota, C. Wong, A. Ooki, Takao Shirai, Wen-Yi Chou, M. Tamiya, H. Tabuse, Y. Kaneko, Y. Shimizu, Y. Murata, A. Okada, S. Sasada, Y. Takagi, A. Naitou, N. Katayama, Kaori Ito, T. Araki, Y. Fujiwara, H. Yokota, Shinya Kajiura, M. Imano, T. Iwai, T. Kobayashi, T. Kubota, N. Kanaji, M. Ohdate, T. Tsukamoto, S. Zenda, A. Fukutomi, T. Kumura, R. Ogawa, K. Shintaku, Kazuto Nishio, T. Morimoto, W. Shioyama, E. K. Cho, H.-I. Lu, Y. Suginoshita, K. Yamaguchi, Y. Shindo, N. Hirokami, J. Shimizu, Chihiro Makimura, K. Araki, T. Taniyama, T. Tanaka, Y. Tanbo, Hiroto Miwa, Y. Hirai, J. Park, Asao Hirose, M. Doi, A. Goto, S. Nomura, S. Ikegaya, A. Yoshii, M. Akahane, T. Kakuma, K. Miyabayashi, S. Y. Kim, H. Kitade, B. Han, K. Yamada, Tadayuki Oshima, J. Ishizawa, M. Miyata, E. Sasak, R. Aibara, N. Takahara, S. Kanno, T. Kojima, I. Ohno, E. Sasaki, E. Tone, A. Morita, R. Suzuki, Yukio Hosomi, Hiroo Ishida, T. Akimoto, N. Hashimoto, T. Takakuwa, K. Umekawa, A. Toyoshima, K. Hara, J. Kitagawa, H. Taniguchi, T. Kamiya, M. Takai, Y. Watanabe, Yasuhito Tanaka, A. Sawada, T. Yasui, Y. Onozawa, Akihiro Hirakawa, S. Okamoto, K. K. Kim, Y.-M. Wang, Y. Takai, T. Tsumura, H. Hirama, Shigeo Horiike, K. Kawasumi, N. Shimeno, Junya Kuroda, C.-Y. Huang, Y.-H. Chen, H. Ogata, S. Matsumoto, I. Takahashi, Hideo Tomioka, I. Okamoto, Itaru Endo, T. M. S. Kam, K. Sekihara, C.-T. Liu, K. Chikamori, N. Hirota, K. Hiramatsu, D. Hamaguchi, T. Nishii, N. Ohmiya, T. Shimizu, T. Sakaizawa, Hiromichi Matsuoka, K. Kawa, J. H. Ji, S. Izumi, T. Hara, Y. Tsuyumu, T. Oguri, T. Akiyama, Y. Ichida, A. Simoyama, T. Hirakata, Y. Yoshimitsu, Y. Sasaki, T. Yamazaki, T. Tsushima, R. Okamoto, Y. Tsukioka, Nobuhiko Seki, S.-M. Bang, Y. Kubota, N. Harada, C.-H. Huang, J. Y. Hong, T. Andou, T. Shimada, T. Doi, Yoshihiro Ono, S. Nanjo, H. Hara, Y. Kikukawa, M. K. Choi, K.-M. Rau, Y. Tomizawa, O. Maeda, K. Ishida, Y. Naito, N. Machida, T. Otsuka, T. Hase, H. Morishita, K. Fukuhara, M. Yoshino, M. Takahashi, H. Takahashi, Heui June Ahn, M. Nisimoto, Y. Sunakawa, Y. Miyakawa, Choung Soo Kim, S.-W. Wang, Takashi Sone, M. Iguchi, T. Shimokawa, Tomoyuki Nagai, K. Morioka, A. Numata, R. Toyozawa, R. Miyahara, Y. M. Ahn, Hyo Song Kim, D. W. Hwang, H. Takamori, Shin-Hee Lee, Narikazu Boku, T. Mizuno, N. Katakami, J. H. Lee, Y. Okuma, Koji Kurokawa, K. Takeda, N. Sakiyama, R. Tachikawa, Satoshi Morita, T. K. Fai, K. H. Seong, K. Yorozu, T. Okamura, Ryo Takahashi, T. Kotake, Y. Arai, T. Kawamura, K. Yakushijinn, Y. Shimada, H. Sugiyama, S. Kamachi, A. Mugitani, T. Yasue, Y. Sugihara, S. Shu, Y. Osaki, Kazuhisa Takahashi, Y. Hashiguchi, K. Funasaka, Y. S. Koo, Tohru Ohmori, S. J. Koh, N. Kanemura, H. Kotani, M. Hsin, T. Kagoo, and A. Inoue

Subjects
biology, Molecular mass, business.industry, Angiogenesis, Hematology, Cell biology, chemistry.chemical_compound, Oncology, chemistry, Proteoglycan, Chondroitin sulfate proteoglycan, Cancer cell, medicine, biology.protein, Hepatocyte growth factor, Antibody, Cell adhesion, business, medicine.drug
Abstract

SRPX2 (Sushi repeat-containing protein, X-linked 2) has recently emerged as a multifunctional protein that is involved in seizure disorders, angiogenesis and cellular adhesion. Here, we analyzed this protein biochemically. SRPX2 protein was secreted with a highly post-translational modification. Chondroitinase ABC treatment completely decreased the molecular mass of purified SRPX2 protein to its predicted size, whereas heparitinase, keratanase and hyaluroinidase did not. Secreted SRPX2 protein was also detected using an anti-chondroitin sulfate antibody. These results indicate that SRPX2 is a novel chondroitin sulfate proteoglycan (CSPG). Furthermore, a binding assay revealed that hepatocyte growth factor dose-dependently binds to SRPX2 protein, and a ligand–glycosaminoglycans interaction was speculated to be likely in proteoglycans. Regarding its molecular architecture, SRPX2 has sushi repeat modules similar to four other CSPGs/lecticans; however, the molecular architecture of SRPX2 seems to be quite different from that of the lecticans. Taken together, we found that SRPX2 is a novel CSPG that is overexpressed in gastrointestinal cancer cells. Our findings provide key glycobiological insight into SRPX2 in cancer cells and demonstrate that SRPX2 is a new member of the cancer-related proteoglycan family.

Published
2012
Full Text
View/download PDF

10. Lectinhistochemistry of the cervical malignancies

Author

H, Sugimori, Y, Ookuma, T, Iwasaka, and Y, Kidera

Subjects
Histocytochemistry, Lectins, Carcinoma, Squamous Cell, Humans, Uterine Cervical Neoplasms, Female, Adenocarcinoma
Published
1986

11. Oxidative stress caused by a low concentration of hydrogen peroxide induces senescence-like changes in mouse gingival fibroblasts.

Author

Kiyoshima T, Enoki N, Kobayashi I, Sakai T, Nagata K, Wada H, Fujiwara H, Ookuma Y, and Sakai H

Subjects
Animals, Cell Proliferation, Cell Shape, Cell Survival, Cells, Cultured, Cyclin-Dependent Kinase Inhibitor p21 genetics, Cyclin-Dependent Kinase Inhibitor p21 metabolism, Fibroblasts drug effects, Fibroblasts metabolism, Gene Expression, Mice, Mice, Inbred BALB C, Phosphorylation, Protein Processing, Post-Translational, Proto-Oncogene Proteins c-mdm2 genetics, Proto-Oncogene Proteins c-mdm2 metabolism, Tumor Suppressor Protein p53 genetics, Tumor Suppressor Protein p53 metabolism, beta-Galactosidase metabolism, Cellular Senescence, Fibroblasts physiology, Gingiva cytology, Hydrogen Peroxide pharmacology, Oxidants pharmacology, Oxidative Stress
Abstract

Periodontal tissue deteriorates under persistent oxidative stress induced by inflammatory reactions in the microflora of the oral cavity. This study aimed to evaluate the cellular properties of mouse gingival fibroblasts (MGFs) in the presence of oxidative stress. MGFs from 10-, 30- and 52-week-old mice were used to evaluate the changes in the cellular properties with aging. The study investigated the effects of oxidative stress on the cellular properties of MGFs from 10-week-old mice. The expression of p53, p21 and murine double minute 2 (Mdm2) in the MGFs in response to oxidative stress was also examined. By day 8, the number of MGFs increased in culture. However, the increase was markedly lower in MGFs derived from aged mice. Oxidative stress due to hydrogen peroxide (H2O2)-induced morphological changes characterized by a round shape with enlarged nuclei and expanded cytoplasm. The cell number of MGFs was decreased subsequent to treatment with 50 µM or a higher concentration of H₂O₂. MGFs treated with H₂O₂ at 20 µM showed a similar cell growth curve as the one seen in 52-week-old mice. Phosphorylated p53 protein was increased in MGFs subsequent to treatment with 20 µM H2O2, along with an upregulated transcription of p21 and Mdm2 mRNAs. These results suggest that treatment with a lower concentration of H₂O₂ in MGFs induces cell cycle arrest, resulting in stress-induced premature senescence, possibly correlated with the development of periodontal diseases.

Published
2012
Full Text
View/download PDF

12. In situ expression of the mitochondrial ATPase6 gene in the developing tooth germ of the mouse lower first molar.

Author

Honda JY, Kobayashi I, Kiyoshima T, Nagata K, Wada H, Ookuma Y, Fujiwara H, Shiotsuka M, Takahashi I, and Sakai H

Subjects
Adenosine Triphosphatases genetics, Animals, Female, Genes, Mitochondrial genetics, In Situ Hybridization, Male, Mice, Mice, Inbred BALB C, Odontogenesis genetics, RNA, Messenger metabolism, Tooth Germ cytology, Adenosine Triphosphatases metabolism, Gene Expression Regulation, Developmental, Molar embryology, Tooth Germ embryology
Abstract

We previously performed cDNA subtraction between the mouse mandibles on embryonic day 10.5 (E10.5) in the pre-initiation stage of the odontogenesis and E12.0 in the late initiation stage to identify genes expressed at its beginning. Adenosine triphosphate synthase subunit a (Atpase6) is one of the highly expressed genes in the E12.0 mandible including tooth germs. In situ hybridization was conducted using the mouse mandibular first molar from E10.5 to E18.0 to determine the precise expression patterns of Atpase6 mRNA in the developing tooth germ. Atpase6 mRNA was strongly expressed in the presumptive dental epithelium and the underlying mesenchyme at E10.5, and in the thickened dental epithelium at E12.0 and E13.0. Strong in situ signals were observed in the epithelium at E14.0, and in the enamel organ excluded the area of the primary enamel knot at E15.0. Atpase6 was strongly expressed in the inner enamel epithelium, the adjacent stratum intermedium, and the outer enamel epithelium in the cervical loops from E16.0 to E18.0. In addition, strong Atpase6 signals were coincidently demonstrated in various developing cranio-facial organs. These results suggest that Atpase6 participates in the high energy-utilizing functions of the cells related to the initiation and the development of the tooth germ as well as those of the other cranio-facial organs.

Published
2011
Full Text
View/download PDF

13. Protogenin, a new member of the immunoglobulin superfamily, is implicated in the development of the mouse lower first molar.

Author

Takahashi KF, Kiyoshima T, Kobayashi I, Xie M, Yamaza H, Fujiwara H, Ookuma Y, Nagata K, Wada H, Sakai T, Terada Y, and Sakai H

Subjects
Animals, Bone Morphogenetic Protein 4 metabolism, Down-Regulation, Embryo, Mammalian metabolism, Gene Expression Regulation, Developmental, Mandible metabolism, Membrane Proteins chemistry, Membrane Proteins genetics, Mice, Mandible embryology, Membrane Proteins metabolism, Molar growth & development, Odontogenesis
Abstract

Background: Protogenin (Prtg) has been identified as a gene which is highly expressed in the mouse mandible at embryonic day 10.5 (E10.5) by a cDNA subtraction method between mandibles at E10.5 and E12.0. Prtg is a new member of the deleted in colorectal carcinoma (DCC) family, which is composed of DCC, Neogenin, Punc and Nope. Although these members play an important role in the development of the embryonic central nervous system, recent research has also shed on the non-neuronal organization. However, very little is known regarding the fetal requirement of the non-neuronal organization for Prtg and how this may be associated with the tooth germ development. This study examined the functional implications of Prtg in the developing tooth germ of the mouse lower first molar., Results: Ptrg is preferentially expressed in the early stage of organogenesis. Prtg mRNA and protein were widely expressed in the mesenchymal cells in the mandible at E10.5. The oral epithelial cells were also positive for Prtg. The expression intensity of Prtg after E12.0 was markedly reduced in the mesenchymal cells of the mandible, and was restricted to the area where the tooth bud was likely to be formed. Signals were also observed in the epithelial cells of the tooth germ. Weak signals were observed in the inner enamel epithelial cells at E16.0 and E18.0. An inhibition assay using a hemagglutinating virus of Japan-liposome containing Prtg antisense-phosphorothioated-oligodeoxynucleotide (AS-S-ODN) in cultured mandibles at E10.5 showed a significant growth inhibition in the tooth germ. The relationship between Prtg and the odontogenesis-related genes was examined in mouse E10.5 mandible, and we verified that the Bmp-4 expression had significantly been decreased in the mouse E10.5 mandible 24 hr after treatment with Prtg AS-S-ODN., Conclusion: These results indicated that the Prtg might be related to the initial morphogenesis of the tooth germ leading to the differentiation of the inner enamel epithelial cells in the mouse lower first molar. A better understanding of the Prtg function might thus play a critical role in revealing a precious mechanism in tooth germ development.

Published
2010
Full Text
View/download PDF

14. In situ expression of 15 kDa interferon alpha responsive gene in the developing tooth germ of the mouse lower first molar.

Author

Akhter M, Kobayashi I, Kiyoshima T, Nagata K, Wada H, Ookuma Y, Fujiwara H, Honda JY, and Sakai H

Subjects
Animals, Face, Interferon-alpha metabolism, Mice, Mice, Inbred BALB C, Molar cytology, Molecular Weight, RNA, Messenger genetics, RNA, Messenger metabolism, Tooth Germ cytology, Gene Expression Regulation, Developmental, In Situ Hybridization, Interferon-alpha genetics, Molar embryology, Molar metabolism, Tooth Germ embryology, Tooth Germ metabolism
Abstract

We previously performed cDNA subtraction between the mouse mandibles at embryonic day 10.5 (E10.5) and E12.0 to make a profile of the regulator genes for odontogenesis. Fifteen kDa interferon alpha responsive gene (Ifrg15) is one of several highly-expressed genes in the E12.0 mandible. The current study examined the precise expression patterns of Ifrg15 mRNA in the mouse mandibular first molar by in situ hybridization to evaluate the possible functional roles of this gene in odontogenesis. Ifrg15 mRNA was expressed in the epithelial and mesenchymal tissues of the mandible at E10.5 and E12.0. The Ifrg15 in situ signal was detected in the epithelial bud and the surrounding mesenchyme at E14.0, and was present in the enamel organ including the primary enamel knot, and in the underlying mesenchyme at E15.0. The in situ signal was restricted in the inner and outer enamel epithelia and the stratum intermedium at E16.0. The signal of Ifrg15 mRNA was further restricted to the inner enamel epithelium and the adjacent stratum intermedium at E17.0 and E18.0. Consequently, the expression of Ifrg15 mRNA was localized in the ameloblasts and odontoblasts at postnatal days 1.0 to 3.0. However, the in situ signal was markedly weaker than at the embryonic period. The expression of Ifrg15 mRNA was coincidently observed in various craniofacial organs as well as in the tooth germ. These results suggest that Ifrg15 is closely related to odontogenesis, especially the differentiation of the ameloblasts and odontoblasts, and to the morphogenesis of the craniofacial organs.

Published
2010
Full Text
View/download PDF

15. In situ expression of ribosomal protein L21 in developing tooth germ of the mouse lower first molar.

Author

Xie M, Kobayashi I, Kiyoshima T, Nagata K, Ookuma Y, Fujiwara H, and Sakai H

Subjects
Animals, Female, Gene Expression Regulation, Developmental, In Situ Hybridization, Male, Mice, Mice, Inbred BALB C, Molar cytology, Organ Specificity, RNA, Messenger genetics, RNA, Messenger metabolism, Ribosomal Proteins metabolism, Tooth Germ cytology, Molar embryology, Molar metabolism, Ribosomal Proteins genetics, Tooth Germ embryology, Tooth Germ metabolism
Abstract

We previously performed cDNA subtraction between the mouse mandibles at embryonic day 10.5 (E10.5) in the pre-initiation stage of the odontogenesis and E12.0 in the late initiation stage to investigate the key regulator genes in odontogenesis. Ribosomal protein L21 (Rpl21) is one of differentially expressed genes in the E12.0 mandible. This study examined the precise expression pattern of Rpl21 mRNA in the mouse mandibular first molar by in situ hybridization. Rpl21 mRNA was expressed in the presumptive dental epithelium and the underlying mesenchyme at E10.5, and in the thickened dental epithelium at E12.0. Strong in situ signals were observed in the epithelial bud at E14.0, and in the enamel organ at E15.0. However, either no (E14.0) or only a weak (E15.0) in situ signal was found in the primary enamel knot at these gestational days. Rpl21 was strongly expressed in the inner enamel epithelium, cervical loop and dental lamina from E16.0 to E18.0. In addition, Rpl21 mRNA was also demonstrated in various developing cranio-facial organs. These results suggest that Rpl21 participates in the synthesis of various polypeptides which might be related to the initiation and the development of such tooth germ, and also in the synthesis of enamel components in the presecretory stage of the ameloblast. Rpl21 for protein synthesis might also be related to the morphogenesis of the developing cranio-facial organs.

Published
2009
Full Text
View/download PDF

16. Assessment of lectin binding for prognosis in endometrial carcinoma.

Author

Ookuma Y, Hachisuga T, Iwasaka T, and Sugimori H

Subjects
Adenocarcinoma mortality, Endometrial Neoplasms mortality, Female, Humans, Immunoenzyme Techniques, Neoplasm Staging, Peanut Agglutinin, Prognosis, Survival Analysis, Adenocarcinoma chemistry, Endometrial Neoplasms chemistry, Lectins metabolism, Plant Lectins
Abstract

The binding of Ulex europeus agglutinin-I (UEA-I) and Peanut agglutinin (PNA) was studied in 56 endometrial carcinomas. The positive rates of UEA-I and PNA were 66%. Following neuraminidase treatment, the PNA positive rate increased from 66% to 98% and most tumors stained abundantly. UEA-I staining was unaffected. Using a combined grading system based on architectural and nuclear abnormalities, the PNA positive rates of grade 1, grade 2 and grade 3 tumors were 78%, 70% and 36%, respectively. There was no correlation between UEA-I binding and the grading systems. UEA-I binding correlated significantly with the presence of myometrial invasion and vessel permeation. Metastatic endometrial carcinomas showed a high affinity of tumor cells for UEA-I. Tissues that stained with UEA-I were those from patients with the worst prognosis. PNA and UEA-I reactivities relate to the biologic activity of endometrial carcinomas. UEA-I can serve as a prognostic marker for patients with endometrial carcinoma.

Published
1994
Full Text
View/download PDF

17. Detection of Epstein-Barr virus DNA from a lymphoma-like lesion of the uterine cervix.

Author

Hachisuga T, Ookuma Y, Fukuda K, Iwasaka T, Sugimori H, and Watanabe T

Subjects
Cervix Uteri microbiology, Diagnosis, Differential, Female, Herpesvirus 4, Human genetics, Humans, Hyperplasia diagnosis, Lymphoma diagnosis, Middle Aged, Uterine Cervical Diseases microbiology, Uterine Cervical Neoplasms diagnosis, Cervix Uteri pathology, DNA, Viral analysis, Herpesvirus 4, Human isolation & purification, Lymphoid Tissue pathology, Tumor Virus Infections pathology, Uterine Cervical Diseases pathology
Abstract

The case of a 60-year-old woman in whom a lymphoma-like lesion of the cervix was found during an episode of silent Epstein-Barr virus (EBV) infection is presented. Fractional curettage was performed because of abnormal endometrial smear. The endocervical curettage specimens were diagnosed as highly suggestive of malignant lymphoma, but microscopic examination of a subsequent hysterectomy specimen revealed a benign lymphoid hyperplasia. Those were retrospectively interpreted as a lymphoma-like lesion of the cervix. In the absence of clinical symptoms of infectious mononucleosis, the results of serologic tests for EBV revealed an active EBV infection. EBV DNA was demonstrated in nuclei of large lymphoid cells in endocervical curettage specimens by in situ hybridization. She is alive and well 32 months postoperatively. When female patients with lymphoma-like lesions of the lower genital tract are encountered, examinations for EBV are recommended.

Published
1992
Full Text
View/download PDF

19. Lectinhistochemistry of the cervical malignancies.

Author

Sugimori H, Ookuma Y, Iwasaka T, and Kidera Y

Subjects
Adenocarcinoma analysis, Carcinoma, Squamous Cell analysis, Female, Histocytochemistry, Humans, Lectins, Uterine Cervical Neoplasms analysis, Adenocarcinoma pathology, Carcinoma, Squamous Cell pathology, Uterine Cervical Neoplasms pathology
Published
1986

20. Immunohistochemical properties of the endometrial and cervical adenocarcinoma.

Author

Sugimori H, Ookuma Y, and Iwasaka T

Subjects
Female, Histocytochemistry, Humans, Lectins analysis, Staining and Labeling, Adenocarcinoma immunology, Carcinoembryonic Antigen analysis, Plant Lectins, Soybean Proteins, Uterine Cervical Neoplasms immunology, Uterine Neoplasms immunology
Published
1986

Catalog

Books, media, physical & digital resources
See catalog results