Search

Gamete collection is feasible in Banteng (Bos javanicus) and is essential for conserving this species. The common practice of semen collection in wild animals is using the electroejaculation technique which is considered invasive with lower-quality results. This study aims to develop sperm collection further using an artificial vagina and to investigate the sperm motility of Banteng after being frozen-thawed. A Banteng bull kept in Taman Safari Indonesia was used for this study. Before semen collection, the bull is acclimatized to collection conditions. Semen collection is done once a month for six months. The semen was evaluated according to a method for bovine semen. Cryopreservation of semen was performed using an Andromed® extender, in 0.25 mL Ministraw®, and equilibrated at 4°C for 3 hours, followed by freezing in liquid nitrogen vapor. Quality testing was performed using the CASA IVOS II™. The result showed variations between batches, with the number of mountings 9.83±2.86(7-15) times, semen volume 8.42±1.42 (6-9) mL, straw produced 152±15.23 (130-172) straws, and total motility after thawing 44.28 ±12.14, (26.83-55.96). The quality of frozen semen of the later batch showed better results p

Anoa, which is the endemic animal in Indonesia, its population is unevitably decreasing, therefore anoa included in the list of endangered species by International Union for Conservation of Nature (IUCN). The experiment was aimed to apply the artificial insemination (AI) technique for anoa and to examine their parturition behavior. The experiment involved 2 males and 5 females anoa at Taman Safari Indonesia Bogor. Semen was collected by electroejaculator, then evaluated and diluted in Tris-egg yolk extender. The anesthetized estrous females were inseminated intracervically with extended semen (100x106 sperm/1.0 mL). Females which did not show estrous signs during 2-3 cycles after AI were predicted pregnant, and therefore confirmed by ultrasonography. As a comparison, another predicted-pregnant female after natural mating was scanned. The results showed that intracervical AI resulted pregnancy in one female, and the gestation period of anoa was ranged from 313 d (AI) to 324 d (natural mating). There were three stages of parturition process was observed: Stage 1 was characterized by the abdominal contraction and amniotic membrane rupture, Stage 2 was characterized by the fetal expulsions, and Stage 3 was characterized by the placental expulsions and ingestion of placenta by the dam. The duration of each stage of parturition was 6-8 h (stage 1), 30-60 min (stage 2), and 15-180 min (stage 3). It is concluded that AI technique was applicable to anoa in captivity, and the parturation behavior of anoa was comparable to buffalo and cattle. However, the fetal delivery occurred when the dam was in standing position and the dam ingested the placenta.